LOCUS       DAFFFQ010000001       155379 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000001
VERSION     DAFFFQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 155379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 155379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..155379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.001"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(3..2189)
                     /locus_tag="LQB69_000001"
     CDS             complement(3..2189)
                     /locus_tag="LQB69_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL3524202.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            2407..3090
                     /locus_tag="LQB69_000002"
     CDS             2407..3090
                     /locus_tag="LQB69_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL3524203.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3171..3890)
                     /locus_tag="LQB69_000003"
     CDS             complement(3171..3890)
                     /locus_tag="LQB69_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3524204.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4202..4408)
                     /locus_tag="LQB69_000004"
     CDS             complement(4202..4408)
                     /locus_tag="LQB69_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524205.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4653..5255
                     /locus_tag="LQB69_000005"
     CDS             4653..5255
                     /locus_tag="LQB69_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL3524206.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5396..6676
                     /locus_tag="LQB69_000006"
     CDS             5396..6676
                     /locus_tag="LQB69_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL3524207.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6689..7417
                     /locus_tag="LQB69_000007"
     CDS             6689..7417
                     /locus_tag="LQB69_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3524208.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7486..9009)
                     /locus_tag="LQB69_000008"
     CDS             complement(7486..9009)
                     /locus_tag="LQB69_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL3524209.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9261..10442
                     /locus_tag="LQB69_000009"
     CDS             9261..10442
                     /locus_tag="LQB69_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3524210.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10502..12130)
                     /gene="treC"
                     /locus_tag="LQB69_000010"
     CDS             complement(10502..12130)
                     /gene="treC"
                     /locus_tag="LQB69_000010"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL3524211.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12581..15292)
                     /locus_tag="LQB69_000011"
     CDS             complement(12581..15292)
                     /locus_tag="LQB69_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524212.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15302..16003)
                     /locus_tag="LQB69_000012"
     CDS             complement(15302..16003)
                     /locus_tag="LQB69_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524213.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16189..16788
                     /locus_tag="LQB69_000013"
     CDS             16189..16788
                     /locus_tag="LQB69_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3524214.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16869..17882
                     /locus_tag="LQB69_000014"
     CDS             16869..17882
                     /locus_tag="LQB69_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL3524215.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            17990..18907
                     /gene="rbsK"
                     /locus_tag="LQB69_000015"
     CDS             17990..18907
                     /gene="rbsK"
                     /locus_tag="LQB69_000015"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL3524216.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            18935..19330
                     /gene="rbsD"
                     /locus_tag="LQB69_000016"
     CDS             18935..19330
                     /gene="rbsD"
                     /locus_tag="LQB69_000016"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL3524217.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19330..20703
                     /locus_tag="LQB69_000017"
     CDS             19330..20703
                     /locus_tag="LQB69_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3524218.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21060..23204
                     /locus_tag="LQB69_000018"
     CDS             21060..23204
                     /locus_tag="LQB69_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL3524219.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(23256..24296)
                     /locus_tag="LQB69_000019"
     CDS             complement(23256..24296)
                     /locus_tag="LQB69_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3524220.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(24573..26375)
                     /locus_tag="LQB69_000020"
     CDS             complement(24573..26375)
                     /locus_tag="LQB69_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524221.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(26389..27141)
                     /locus_tag="LQB69_000021"
     CDS             complement(26389..27141)
                     /locus_tag="LQB69_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524222.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(27150..27512)
                     /locus_tag="LQB69_000022"
     CDS             complement(27150..27512)
                     /locus_tag="LQB69_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3524223.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(27688..28560)
                     /locus_tag="LQB69_000023"
     CDS             complement(27688..28560)
                     /locus_tag="LQB69_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL3524224.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(28611..29099)
                     /locus_tag="LQB69_000024"
     CDS             complement(28611..29099)
                     /locus_tag="LQB69_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL3524225.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(29237..30028)
                     /locus_tag="LQB69_000025"
     CDS             complement(29237..30028)
                     /locus_tag="LQB69_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL3524226.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(30083..30691)
                     /locus_tag="LQB69_000026"
     CDS             complement(30083..30691)
                     /locus_tag="LQB69_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL3524227.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLVWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(30684..31001)
                     /locus_tag="LQB69_000027"
     CDS             complement(30684..31001)
                     /locus_tag="LQB69_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3524228.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(31157..31885)
                     /locus_tag="LQB69_000028"
     CDS             complement(31157..31885)
                     /locus_tag="LQB69_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL3524229.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            32044..32925
                     /locus_tag="LQB69_000029"
     CDS             32044..32925
                     /locus_tag="LQB69_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3524230.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(33162..34205)
                     /locus_tag="LQB69_000030"
     CDS             complement(33162..34205)
                     /locus_tag="LQB69_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3524231.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(34208..34876)
                     /locus_tag="LQB69_000031"
     CDS             complement(34208..34876)
                     /locus_tag="LQB69_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3524232.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            35018..37354
                     /locus_tag="LQB69_000032"
     CDS             35018..37354
                     /locus_tag="LQB69_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3524233.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(37469..37780)
                     /locus_tag="LQB69_000033"
     CDS             complement(37469..37780)
                     /locus_tag="LQB69_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524234.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            37961..39436
                     /locus_tag="LQB69_000034"
     CDS             37961..39436
                     /locus_tag="LQB69_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL3524235.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            39483..40067
                     /locus_tag="LQB69_000035"
     CDS             39483..40067
                     /locus_tag="LQB69_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL3524236.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            40069..40488
                     /locus_tag="LQB69_000036"
     CDS             40069..40488
                     /locus_tag="LQB69_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL3524237.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(40769..41053)
                     /locus_tag="LQB69_000037"
     CDS             complement(40769..41053)
                     /locus_tag="LQB69_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL3524238.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(41405..42115)
                     /locus_tag="LQB69_000038"
     CDS             complement(41405..42115)
                     /locus_tag="LQB69_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL3524239.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(42112..43584)
                     /locus_tag="LQB69_000039"
     CDS             complement(42112..43584)
                     /locus_tag="LQB69_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3524240.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            43722..44195
                     /locus_tag="LQB69_000040"
     CDS             43722..44195
                     /locus_tag="LQB69_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3524241.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(44348..44899)
                     /locus_tag="LQB69_000041"
     CDS             complement(44348..44899)
                     /locus_tag="LQB69_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3524242.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            45004..45534
                     /locus_tag="LQB69_000042"
     CDS             45004..45534
                     /locus_tag="LQB69_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL3524243.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(46017..46460)
                     /locus_tag="LQB69_000043"
     CDS             complement(46017..46460)
                     /locus_tag="LQB69_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524244.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(46476..47636)
                     /locus_tag="LQB69_000044"
     CDS             complement(46476..47636)
                     /locus_tag="LQB69_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3524245.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            48143..49843
                     /locus_tag="LQB69_000045"
     CDS             48143..49843
                     /locus_tag="LQB69_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524246.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(49945..50778)
                     /locus_tag="LQB69_000046"
     CDS             complement(49945..50778)
                     /locus_tag="LQB69_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL3524247.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(51082..53247)
                     /locus_tag="LQB69_000047"
     CDS             complement(51082..53247)
                     /locus_tag="LQB69_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3524248.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            53507..55801
                     /locus_tag="LQB69_000048"
     CDS             53507..55801
                     /locus_tag="LQB69_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL3524249.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFKLLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            55794..56459
                     /gene="pgmB"
                     /locus_tag="LQB69_000049"
     CDS             55794..56459
                     /gene="pgmB"
                     /locus_tag="LQB69_000049"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL3524250.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFTKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            56452..57459
                     /locus_tag="LQB69_000050"
     CDS             56452..57459
                     /locus_tag="LQB69_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3524251.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            57481..58500
                     /locus_tag="LQB69_000051"
     CDS             57481..58500
                     /locus_tag="LQB69_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524252.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(58781..60149)
                     /gene="nhaC"
                     /locus_tag="LQB69_000052"
                     /pseudo
     CDS             complement(58781..60149)
                     /gene="nhaC"
                     /locus_tag="LQB69_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
     gene            complement(60258..61679)
                     /locus_tag="LQB69_000053"
     CDS             complement(60258..61679)
                     /locus_tag="LQB69_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3524253.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(61922..63799)
                     /gene="tdc"
                     /locus_tag="LQB69_000054"
     CDS             complement(61922..63799)
                     /gene="tdc"
                     /locus_tag="LQB69_000054"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL3524254.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(64090..65346)
                     /locus_tag="LQB69_000055"
     CDS             complement(64090..65346)
                     /locus_tag="LQB69_000055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL3524255.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(65458..65663)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            65926..67227
                     /locus_tag="LQB69_000056"
     CDS             65926..67227
                     /locus_tag="LQB69_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL3524256.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(67296..67895)
                     /locus_tag="LQB69_000057"
     CDS             complement(67296..67895)
                     /locus_tag="LQB69_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3524257.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            68149..69030
                     /locus_tag="LQB69_000058"
     CDS             68149..69030
                     /locus_tag="LQB69_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524258.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            69043..70653
                     /locus_tag="LQB69_000059"
     CDS             69043..70653
                     /locus_tag="LQB69_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL3524259.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(70730..71389)
                     /locus_tag="LQB69_000060"
     CDS             complement(70730..71389)
                     /locus_tag="LQB69_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3524260.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(71672..72337)
                     /locus_tag="LQB69_000061"
     CDS             complement(71672..72337)
                     /locus_tag="LQB69_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL3524261.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(72484..73089)
                     /gene="dhaL"
                     /locus_tag="LQB69_000062"
     CDS             complement(72484..73089)
                     /gene="dhaL"
                     /locus_tag="LQB69_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL3524262.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(73117..74127)
                     /gene="dhaK"
                     /locus_tag="LQB69_000063"
     CDS             complement(73117..74127)
                     /gene="dhaK"
                     /locus_tag="LQB69_000063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL3524263.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(74102..74548)
                     /gene="dhaM"
                     /locus_tag="LQB69_000064"
     CDS             complement(74102..74548)
                     /gene="dhaM"
                     /locus_tag="LQB69_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL3524264.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            74651..74971
                     /locus_tag="LQB69_000065"
     CDS             74651..74971
                     /locus_tag="LQB69_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL3524265.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(75083..75952)
                     /locus_tag="LQB69_000066"
     CDS             complement(75083..75952)
                     /locus_tag="LQB69_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3524266.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(76048..77352)
                     /locus_tag="LQB69_000067"
     CDS             complement(76048..77352)
                     /locus_tag="LQB69_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3524267.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(77349..77858)
                     /locus_tag="LQB69_000068"
     CDS             complement(77349..77858)
                     /locus_tag="LQB69_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524268.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(78033..80168)
                     /locus_tag="LQB69_000069"
     CDS             complement(78033..80168)
                     /locus_tag="LQB69_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3524269.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(80251..81906)
                     /locus_tag="LQB69_000070"
     CDS             complement(80251..81906)
                     /locus_tag="LQB69_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3524270.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(81908..82222)
                     /locus_tag="LQB69_000071"
     CDS             complement(81908..82222)
                     /locus_tag="LQB69_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL3524271.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            82392..82652
                     /locus_tag="LQB69_000072"
     CDS             82392..82652
                     /locus_tag="LQB69_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL3524272.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            82666..82980
                     /locus_tag="LQB69_000073"
     CDS             82666..82980
                     /locus_tag="LQB69_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL3524273.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(83710..84264)
                     /locus_tag="LQB69_000074"
     CDS             complement(83710..84264)
                     /locus_tag="LQB69_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL3524274.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(84390..86319)
                     /locus_tag="LQB69_000075"
                     /pseudo
     CDS             complement(84390..86319)
                     /locus_tag="LQB69_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(86625..87341)
                     /locus_tag="LQB69_000076"
     CDS             complement(86625..87341)
                     /locus_tag="LQB69_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL3524275.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(87342..89165)
                     /gene="glpO"
                     /locus_tag="LQB69_000077"
     CDS             complement(87342..89165)
                     /gene="glpO"
                     /locus_tag="LQB69_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL3524276.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(89179..90675)
                     /gene="glpK"
                     /locus_tag="LQB69_000078"
     CDS             complement(89179..90675)
                     /gene="glpK"
                     /locus_tag="LQB69_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL3524277.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(90811..92271)
                     /locus_tag="LQB69_000079"
     CDS             complement(90811..92271)
                     /locus_tag="LQB69_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524278.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(92261..93556)
                     /locus_tag="LQB69_000080"
     CDS             complement(92261..93556)
                     /locus_tag="LQB69_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3524279.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(93888..94565)
                     /locus_tag="LQB69_000081"
     CDS             complement(93888..94565)
                     /locus_tag="LQB69_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL3524280.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(94595..95686)
                     /locus_tag="LQB69_000082"
     CDS             complement(94595..95686)
                     /locus_tag="LQB69_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL3524281.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(95787..96221)
                     /locus_tag="LQB69_000083"
     CDS             complement(95787..96221)
                     /locus_tag="LQB69_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3524282.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(96805..98262)
                     /gene="ascB"
                     /locus_tag="LQB69_000084"
     CDS             complement(96805..98262)
                     /gene="ascB"
                     /locus_tag="LQB69_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL3524283.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(98259..100154)
                     /locus_tag="LQB69_000085"
     CDS             complement(98259..100154)
                     /locus_tag="LQB69_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3524284.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(100212..101987)
                     /locus_tag="LQB69_000086"
     CDS             complement(100212..101987)
                     /locus_tag="LQB69_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3524285.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(102037..103533)
                     /locus_tag="LQB69_000087"
     CDS             complement(102037..103533)
                     /locus_tag="LQB69_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3524286.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(103727..105349)
                     /locus_tag="LQB69_000088"
     CDS             complement(103727..105349)
                     /locus_tag="LQB69_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL3524287.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGGDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(105677..106990)
                     /locus_tag="LQB69_000089"
     CDS             complement(105677..106990)
                     /locus_tag="LQB69_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL3524288.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(107235..107459)
                     /locus_tag="LQB69_000090"
     CDS             complement(107235..107459)
                     /locus_tag="LQB69_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524289.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(107479..107901)
                     /locus_tag="LQB69_000091"
     CDS             complement(107479..107901)
                     /locus_tag="LQB69_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL3524290.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(108044..108754)
                     /locus_tag="LQB69_000092"
     CDS             complement(108044..108754)
                     /locus_tag="LQB69_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524291.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(108772..109269)
                     /locus_tag="LQB69_000093"
     CDS             complement(108772..109269)
                     /locus_tag="LQB69_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524292.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(109306..109758)
                     /locus_tag="LQB69_000094"
     CDS             complement(109306..109758)
                     /locus_tag="LQB69_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3524293.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(109893..110498)
                     /locus_tag="LQB69_000095"
     CDS             complement(109893..110498)
                     /locus_tag="LQB69_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3524294.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(110948..111568)
                     /locus_tag="LQB69_000096"
     CDS             complement(110948..111568)
                     /locus_tag="LQB69_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL3524295.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(111570..112439)
                     /locus_tag="LQB69_000097"
     CDS             complement(111570..112439)
                     /locus_tag="LQB69_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL3524296.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(112462..112869)
                     /locus_tag="LQB69_000098"
     CDS             complement(112462..112869)
                     /locus_tag="LQB69_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL3524297.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(112888..114177)
                     /locus_tag="LQB69_000099"
     CDS             complement(112888..114177)
                     /locus_tag="LQB69_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3524298.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(114204..114506)
                     /locus_tag="LQB69_000100"
     CDS             complement(114204..114506)
                     /locus_tag="LQB69_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3524299.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(114531..116624)
                     /locus_tag="LQB69_000101"
     CDS             complement(114531..116624)
                     /locus_tag="LQB69_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3524300.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(117113..117877)
                     /locus_tag="LQB69_000102"
     CDS             complement(117113..117877)
                     /locus_tag="LQB69_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL3524301.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(117988..118857)
                     /gene="mdcB"
                     /locus_tag="LQB69_000103"
     CDS             complement(117988..118857)
                     /gene="mdcB"
                     /locus_tag="LQB69_000103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL3524302.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(118882..119958)
                     /locus_tag="LQB69_000104"
     CDS             complement(118882..119958)
                     /locus_tag="LQB69_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL3524303.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(119982..120575)
                     /locus_tag="LQB69_000105"
     CDS             complement(119982..120575)
                     /locus_tag="LQB69_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL3524304.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(120579..122255)
                     /gene="mdcD"
                     /locus_tag="LQB69_000106"
     CDS             complement(120579..122255)
                     /gene="mdcD"
                     /locus_tag="LQB69_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL3524305.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(122245..122544)
                     /locus_tag="LQB69_000107"
     CDS             complement(122245..122544)
                     /locus_tag="LQB69_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL3524306.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(122646..124292)
                     /gene="mdcA"
                     /locus_tag="LQB69_000108"
     CDS             complement(122646..124292)
                     /gene="mdcA"
                     /locus_tag="LQB69_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL3524307.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(124299..125210)
                     /locus_tag="LQB69_000109"
     CDS             complement(124299..125210)
                     /locus_tag="LQB69_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL3524308.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            125330..126187
                     /locus_tag="LQB69_000110"
     CDS             125330..126187
                     /locus_tag="LQB69_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3524309.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(126374..126736)
                     /locus_tag="LQB69_000111"
     CDS             complement(126374..126736)
                     /locus_tag="LQB69_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL3524310.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            127082..128521
                     /locus_tag="LQB69_000112"
     CDS             127082..128521
                     /locus_tag="LQB69_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524311.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(128528..131080)
                     /locus_tag="LQB69_000113"
     CDS             complement(128528..131080)
                     /locus_tag="LQB69_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL3524312.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(131194..132525)
                     /locus_tag="LQB69_000114"
     CDS             complement(131194..132525)
                     /locus_tag="LQB69_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3524313.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(132491..133969)
                     /locus_tag="LQB69_000115"
     CDS             complement(132491..133969)
                     /locus_tag="LQB69_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3524314.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(134274..136109)
                     /gene="tdc"
                     /locus_tag="LQB69_000116"
     CDS             complement(134274..136109)
                     /gene="tdc"
                     /locus_tag="LQB69_000116"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL3524315.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            136579..138006
                     /locus_tag="LQB69_000117"
     CDS             136579..138006
                     /locus_tag="LQB69_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524316.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(138098..138463)
                     /locus_tag="LQB69_000118"
     CDS             complement(138098..138463)
                     /locus_tag="LQB69_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL3524317.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(138460..138825)
                     /locus_tag="LQB69_000119"
     CDS             complement(138460..138825)
                     /locus_tag="LQB69_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL3524318.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(139017..139265)
                     /locus_tag="LQB69_000120"
     CDS             complement(139017..139265)
                     /locus_tag="LQB69_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524319.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            139264..139392
                     /locus_tag="LQB69_000121"
     CDS             139264..139392
                     /locus_tag="LQB69_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL3524320.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            139449..141143
                     /locus_tag="LQB69_000122"
                     /pseudo
     CDS             139449..141143
                     /locus_tag="LQB69_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
     gene            141147..141266
                     /locus_tag="LQB69_000123"
                     /pseudo
     CDS             141147..141266
                     /locus_tag="LQB69_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            141917..143050
                     /locus_tag="LQB69_000124"
     CDS             141917..143050
                     /locus_tag="LQB69_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL3524321.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            143081..143401
                     /locus_tag="LQB69_000125"
     CDS             143081..143401
                     /locus_tag="LQB69_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL3524322.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(143430..143894)
                     /locus_tag="LQB69_000126"
     CDS             complement(143430..143894)
                     /locus_tag="LQB69_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524323.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(144026..145280)
                     /locus_tag="LQB69_000127"
                     /pseudo
     CDS             complement(144026..145280)
                     /locus_tag="LQB69_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(145309..145626)
                     /locus_tag="LQB69_000128"
     CDS             complement(145309..145626)
                     /locus_tag="LQB69_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3524324.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(145652..145972)
                     /locus_tag="LQB69_000129"
     CDS             complement(145652..145972)
                     /locus_tag="LQB69_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3524325.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(146436..147851)
                     /locus_tag="LQB69_000130"
     CDS             complement(146436..147851)
                     /locus_tag="LQB69_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3524326.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(147868..149184)
                     /locus_tag="LQB69_000131"
     CDS             complement(147868..149184)
                     /locus_tag="LQB69_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3524327.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(149334..150032)
                     /locus_tag="LQB69_000132"
     CDS             complement(149334..150032)
                     /locus_tag="LQB69_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL3524328.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(150321..151277)
                     /locus_tag="LQB69_000133"
     CDS             complement(150321..151277)
                     /locus_tag="LQB69_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3524329.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(151390..152223)
                     /gene="msrA"
                     /locus_tag="LQB69_000134"
     CDS             complement(151390..152223)
                     /gene="msrA"
                     /locus_tag="LQB69_000134"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3524330.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(152398..154026)
                     /locus_tag="LQB69_000135"
     CDS             complement(152398..154026)
                     /locus_tag="LQB69_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524331.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIRLVSKGFKNADFIAI
                     FKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDL
                     TTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAKLL
                     QFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQK
                     IISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPY
                     KTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGVAD
                     IKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(154013..155347)
                     /locus_tag="LQB69_000136"
     CDS             complement(154013..155347)
                     /locus_tag="LQB69_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524332.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        45872 a        32102 c        26968 g        50437 t
ORIGIN      
        1 tatcactttc cttttttcac acgactcccg gtttctttga taaatctcga tactttcaat
       61 tttcttgaat ttttcatttg aggtacacat agatacagac gatttcttgc tctagtcatc
      121 gcaacataaa atagtcttcg ttcttcttct atcgtcgtta ctggttctgc ttttatttca
      181 ttaaagtcga ttactttctt ttctgtcgct ccgttataag gcaaacttcc ttcgttacaa
      241 ccaatcaaaa agacatttcg gtaactaaga cctttacttt tgtgaatgct ctggatacat
      301 agtgcatcaa gttttccttg tctgtaagca atcaagcggg tttttaattc tttagctgtc
      361 gcttgggttt cttgcgataa ggcttggagt cttctgaaag tagcatgctg ctttacttct
      421 tctcgtatcc attcttgatc actctggtcg atttccttag ctatacgtgg cagatgaaga
      481 tagctttgaa caagcgaata gatttcttga tgctggtctg ttttacgcaa cgcttcctca
      541 aactctcgga tttctgcaga tttcgatttt aaattggggc ttatcgacaa aatataatct
      601 gcaacggata tattttgata tttaggagaa aagtattgat cgcattcact ttttttcagg
      661 tagcagtctg ccatcagctg ccggagcaat tgctggtagg cctgattttt cttttgcaga
      721 ttcagtgctg tttgccaatg aacaattttt tgatagaaat gttggaaaga cttgatacca
      781 aagatacctt catatggact atctgcttcc agtacaaaag gaatattcgt tttcagcaga
      841 gattggacaa gctgctgcgt ttgtgaagct gatcggtata atatgattga ttccttatat
      901 ttcccatttt tttgaataaa tgtctgaatt tgtttgatta gccactgact ttctgcttta
      961 gtatcagaaa acaaataggc ttccataaca cctgcttctt tttgtgctgg aatcatcgcc
     1021 tttggtatcc gatcattttt attatagctg atcaatctat ttgctccttg gacaatagct
     1081 gcccgagaac gataattagt taataagaaa agagttttgg taggatattc tttttcaaag
     1141 ttcttgatgt atgctggctc tgatcctctg aatccataga tactctgatc atcatctcca
     1201 acaacaatca acttgatcct gttttccttt gtcatccgtt tcacgatttc ccattgaagt
     1261 ggattagtat cttgaaattc atcaatgaat agataatcat atttttggcc gatataatgc
     1321 tggatctcct cattctccaa agcgcttttc agattttgga gtacatcatt gaactctagc
     1381 aattgctggc ttcttttttg ttcttggagt tccgtgagca atccatacag tcgttctgat
     1441 atcggctgtc gtgcattctt atcaatggaa tcatattttt ctttaactaa ctgaaccatt
     1501 tcttctactg aaaggttcat attctgccaa tagtctaacc gagaagcaat cgttttttct
     1561 ccatattgat caaatatata aagattatat ttacgtatca gctgttgcaa aacagcattc
     1621 actttttgtg cagtatcctt tgaaaagtct ccttgtattc cttgcattgc tacttcagga
     1681 aattttcgac aaatttcatt gtatatccta aaaaagatac tatggaatgt tccgatatgg
     1741 acagcggttt ttctctggct gaaattatct actcgttttt ttagttcatt tgcagcgttc
     1801 cttgtaaaag tgatcattag tatgcgttct tcgtctactt gtttttgcgt catcagataa
     1861 acagcttttg cacagattgt ttgcgttttg ccgctcccag ctcctgctat gattcgaaga
     1921 tgtttcttca tatcatatgt gatagctgct ttttgttctt gattgaagcg attataggaa
     1981 cgtttctcca tcattttaaa gaaagagctg tttccttcac tttcttttac ttgtacttct
     2041 tcctcaataa gtccgggtgc ttcgccataa gccgcttcat aggtagcaat gatttcttct
     2101 tgtagccgaa agacttgttg aagcttttta tacttgttct cccagcgttt caattctttt
     2161 tgtaatcgga gaatttcttt ttcattcaac tgattgattt ccttcccatc tttcttcttt
     2221 gaatttgttt atttcaagtt ggttatttaa agtttcgttg tctatttttt ctttatacta
     2281 acagaccttt ctaaaaatga aaatgggaag cgatatgatt ttagttttct gaaaattttt
     2341 cctttttttc aatcgtttat gctaagataa ggaagacaaa aatattcata aaaggtgatg
     2401 aaaaaaatgc agacttcctt tcacgagggg cgtacaatag gacttttgct gacatttatc
     2461 ggaggagcca tggacagtta tacttatatc caatatggtg cttttgcctc tgctcaaact
     2521 ggcaatatca ttctatcgat catccaagca tttgacggag aatggctaag tgttggtaaa
     2581 aaagtattgt ctaccctttt ctttttctta gggattcttc taacaaagta tctaattgat
     2641 tattttagaa aaaaagaaaa acatttctgg cgcttgtttg ttctatatta tgaagcagtg
     2701 atttttttct ttgttagttt gacacccgtc catgagcatc ccgcattagt aacgattttg
     2761 attgctttta ctgcatcgat ccagtgggta gcttttgata aaatcaacgg gcgtgcctac
     2821 actaatttat ttacaactgg aaatttaaaa ggggtagcaa cgaacttata tgattattgt
     2881 atttctaaag aaaaagcaga ttttgatcga tttttccact atctaagtgt cgttcttgcc
     2941 tttatcgcag gagctattgt ccttgttttc tgtcatcatc tgtttggcgc ccattcgatt
     3001 ttgatcgttt ccattttatt tttgatcatg gccctcactc agacatttca tttatggcaa
     3061 ttttatcata cgaatcggtt cactatttga tctcaatgaa tcaaaaaaac taagcaagat
     3121 gctgctcaca gagaaatatc tgcgagtgag caatttgctt agtttttata ttatttagtt
     3181 gtaaagattc cctttgcttt cgggttattt gtagtgtagc ggatcgactg ggcatctgcc
     3241 tcttctgtca ttttctttgc ccgcatcttg atttcttgta cgatttgttc tccacatgcc
     3301 ggatcggcta cgaaatcata tttgtcccca tcgatcacta ttttgggaca tatatcaaac
     3361 tgttcatacc actcttcgta tcgtcgattg agttctttat agtaggaata gagttcagga
     3421 tcttgttcta gctgttcata ttcccggcct ctttttttga ttctcgctaa catagtttca
     3481 aaggatacac gaatataaac cagtagatct ggtttctttt gtggcgaaat ttcttctaac
     3541 tccttcagca tcgtttctaa aagaccttca tactgcttta cttctacttc agaaacacgc
     3601 cctaaatctg cattcaaatg aaacagcata ctatcttcgt agatggagcg gtctagtacg
     3661 ttgtcatcat ggctgaaagc atctttgatt gccaaaaagc gtttgttcaa aaagaaaatc
     3721 tgtaataaaa aagtatactt ttctggatta gaataaaata gcggcaatac ttcattatct
     3781 tccacgttct cataaaaagg ttttgtttct aaatcttggg caagcatttc agttagtgtg
     3841 cttttccctg caccgatcgt tcctgcaaca atgatcacaa ttatcggcat ccttcctgta
     3901 ataaaatctg ttcttcattt tactacttca tttatagaaa taaaagcctt gactttatct
     3961 gctccttaaa agctacttct ctaaaagtag ccaaaagtaa ctcctattca tgaaaataaa
     4021 ggctgtgaca aaagtcttgt cgcagccctt ttttcaaata aacagtattc tcctgccaac
     4081 acctcggtca caaggcaata ttcataaaac atgaaaagct gttttttaac attattcctt
     4141 gtaactcaga gctaaacggg agactcacaa cctctttatg ctctcaacat tttatctgtt
     4201 tttattcttc tattgtactt gatttgcgta caactaactg ccagtgcaga ttatgagcgg
     4261 tatcattgtc ttctactgcg catttcatga cacgtatcat cacactattt tcatagattt
     4321 tttctatttt tccttttatt ggtgattcaa ataaatcaga tttaaatgca tatgtttcgc
     4381 cgacttcaaa caattgttca ttttccataa gaatccctcc atcacctttt ataaggtcag
     4441 taatttttta acataaatca ctcgaaaaag cacaaacttg tcataaaaag tacttttcgt
     4501 atcaaaactg tcactttttt tggtaaggtt cttggaaaac gaagattttt ttaggagatc
     4561 atgacggttt tgatgtcaaa accacttttt atgaaacaaa agacgttttt tatttttttt
     4621 agcataaaat ttgtggtgga ggtacgtatg agatgaaaga taaagttgtg aacaaattat
     4681 tagaagcctt attcagcgga gacaaatcgg aagaagatat ggtgtatgtc caagtaaaat
     4741 ttgtcttaga agttttattg aataatcttg gaaaactagc ggtcgttcta gtcttttctc
     4801 tggtgacagg ctcatgggcg gaaacaggaa tcacctttct ttcttatatc tgcatccgca
     4861 gatatgctta tggattgcat tccgactctg aattcgtttg tctgctgtgg acactacttt
     4921 atttatgggg cgttcccttg gtgatgaaac atctgcaatt gactatctcc ttcccaatga
     4981 tggtcttttt gttaatcagc tgctttttgt tactgcttcg ctatggatca agaggtacag
     5041 caatcaatcc cattgaaccc gagaaacgtc ccccattatt aaagaaggcg atcagcatgt
     5101 ttctaatttt ttctctcatt acgttatttt tctcagcttc ttacttttct acctatttgc
     5161 tacttgggat tgttttagaa atagctacgt tgcttcctat tacaaactac ttgatgaatt
     5221 ttggaggaat ctttcatgaa aaaaacaatc attaaacaat taactaactt atctgacttt
     5281 tttgtatctg gctgctgctt tggtatgagt tctgaattca aaatccctga aaaattaaaa
     5341 aaataatacg ccaattgtgc tataatttgg taaaaatcaa tgggagctgt actttatgtt
     5401 tttatggtcc atagcgttgc aatatttgat ttacacaatt tgtatcttat tgattgacca
     5461 tcacatccga tacaaatatc tgtttgcggg agtcattctg atttcttgta ttttgattgg
     5521 atgggtctat tcgattttag ggatttttac tgctctaatt gctataggaa tgctgatgct
     5581 tacttttttc ttcttttcga cttcgcgtca acaattggtt tacgcgcttc cttttgggtt
     5641 attgacgtcg cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc
     5701 ttcggttatt gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt
     5761 atctgcttta ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga
     5821 ccgccgtatg atcggtacga tcgggacatt gatattattt acatactata tcattatttt
     5881 ttatacccgt ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat
     5941 tctatatctg tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa
     6001 attagctgat cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct
     6061 agaaaaaaac tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc
     6121 tcttcacagc tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac
     6181 gatattacct acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa
     6241 tatgaaagtt cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa
     6301 agggttgatc gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt
     6361 aaaccttgta cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc
     6421 aaagaagaaa aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt
     6481 gatcaataca acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac
     6541 aaaaacaaat ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac
     6601 caatccttat cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta
     6661 tatacaaata aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc
     6721 aaaacaatta gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga
     6781 cagttatgtc catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag
     6841 aacttcttct tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg
     6901 tgtagcgatc ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac
     6961 ttcccatacg gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat
     7021 cgtcaaagaa gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt
     7081 cgaagatttt caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga
     7141 gatcaaattt ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca
     7201 taaactggaa gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat
     7261 cgagaaaatg catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa
     7321 tgtgcggtcg attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc
     7381 agcctctatc cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg
     7441 actaaatgac cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat
     7501 ttcctgtgca atttcacgtc catccattgt atctggataa aatgttggcc aattctccat
     7561 ttctttcagc aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc
     7621 agccggtgca atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc
     7681 ttctgcttcg aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt
     7741 ccctacataa cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc
     7801 tttctcactg atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt
     7861 atattcttgt gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa
     7921 agtcccatct tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga
     7981 gcgatcttgt acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg
     8041 ggtcaaagta tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt
     8101 tgcttccatg atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc
     8161 caaaggattc aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact
     8221 atctttggcg ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt
     8281 cagttcagca attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac
     8341 ttgattcaat ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga
     8401 tctttgtttt gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc
     8461 ggtataggct gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag
     8521 ctgatcacgt atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg
     8581 gtctagctca tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc
     8641 acttcccgga agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg
     8701 taatgttttt tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat
     8761 atctgtatct tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt
     8821 ccctgctgga atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc
     8881 atacatggga taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc
     8941 acttgccccg caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa
     9001 tcgtttcatt tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt
     9061 ttcgcaaacc gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag
     9121 aactgtcaag cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata
     9181 tttttgcaat caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta
     9241 tagataaagg aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct
     9301 ttttaggagg aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca
     9361 tttatttttt ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta
     9421 tcttgccccc attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac
     9481 gtttgagcaa gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat
     9541 ttttattggg gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat
     9601 tgatcagtag tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg
     9661 agaaaacacc ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt
     9721 ctgatatagc tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca
     9781 ttttttcaac tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt
     9841 tccttttgag agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat
     9901 ttcgtacaga ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa
     9961 aggggcaaat cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga
    10021 tcggattaga atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat
    10081 atattgggcc gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt
    10141 ttatggctgt caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg
    10201 tgattcctcg agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt
    10261 tacttgtcat gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata
    10321 cgttagtcaa attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat
    10381 tttatggcga taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat
    10441 aaaacacaaa acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt
    10501 tttaattttt cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa
    10561 cagtatcgga ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga
    10621 tccttgtatc tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc
    10681 gaagaaagcc ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa
    10741 cgattggata tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt
    10801 cagaagatag ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag
    10861 gtttccctgt agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt
    10921 tatcgcgaga ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa
    10981 ggagtaactt ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt
    11041 ctgggtcgat catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat
    11101 ggatcatggt tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc
    11161 gattgagcgc acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct
    11221 cactcatctt ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag
    11281 gagtagacca tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca
    11341 ttgataattc ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc
    11401 tcatttcacc gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt
    11461 ttagatacgt atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat
    11521 tatttttcaa ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta
    11581 cccctttttc catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc
    11641 gccagttcaa gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc
    11701 caaatggcga ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttctctta
    11761 aaatgaagaa gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt
    11821 ctgtcgaaac gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt
    11881 cggctattag ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat
    11941 cagagatatc ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca
    12001 tgtcgatgcc tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa
    12061 ttccatcacc attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt
    12121 taaatgtcat ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat
    12181 acaagttatc atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac
    12241 attctaagag gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct
    12301 cttgtttaaa atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat
    12361 ctacggcaat cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct
    12421 ttttgctcga tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt
    12481 ccttcggtat tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc
    12541 taatactcag gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg
    12601 catcaatcat atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa
    12661 atacgatagt taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga
    12721 tgacattctc catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa
    12781 atcctccgat gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga
    12841 cttctttatc aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa
    12901 tattgatatt agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa
    12961 agattaagac aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg
    13021 acatgaaaga cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt
    13081 cttcttttgt tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat
    13141 cagtaactga agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca
    13201 cgatttcttt tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat
    13261 ctcctgtaga taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag
    13321 gcaaggagcg tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg
    13381 ttttacttgg tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta
    13441 cggtttgagt atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg
    13501 ctgctttttg acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa
    13561 tgatcgttcc aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg
    13621 caaccatcag cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat
    13681 atcggaagag atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag
    13741 tgatgtattc aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag
    13801 caggccgttc tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga
    13861 agaatgcggc agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa
    13921 tatcataccg ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata
    13981 tcgttcctaa aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg
    14041 cgatttcaac tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt
    14101 ctaccatacg tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag
    14161 gaaaaacatt ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga
    14221 cattttctga acgttcattg tataggtatg atggtttagt taaattttca attttttctg
    14281 catttcgatt caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc
    14341 tttcttgttc agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt
    14401 gcgcttcagg aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt
    14461 ctaaccgtgt tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg
    14521 gttctaattc ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt
    14581 gtaaatactt ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt
    14641 acgtattcaa attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt
    14701 taaaaacgga ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt
    14761 atcctctctc caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact
    14821 ctttttcttt caattcatct gaatcaatct gataaatatc accgatttta tacccttcat
    14881 cttccgcttt ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta
    14941 attgtagctt gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt
    15001 tgccagtatt ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt
    15061 ctgcttgagc aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa
    15121 gtcctgctgt accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga
    15181 cacctacaaa taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt
    15241 tagattgccg aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc
    15301 cctaccatac aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt
    15361 tagcatcatt aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa
    15421 tgatcaaaac ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct
    15481 tgcccgtttc aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt
    15541 tagctagtgc acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa
    15601 agttattcaa tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat
    15661 caggagaaac ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat
    15721 tgtagaattg gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg
    15781 aaaattgcgc aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc
    15841 cacctaaaat attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta
    15901 attcgccttt ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca
    15961 ttttgtatcg tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt
    16021 cacgatattt ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac
    16081 cactgaattt ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt
    16141 tatgatttac ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta
    16201 aaggaaaata tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa
    16261 gagatcgtaa ataacggtac actctctcca cacactgcag catctattct gacaaacaat
    16321 gatttgttag taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac
    16381 atctccactt tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta
    16441 cctccagaat tcactgattt acttttgaag gattctttga aagatacaga taatatgttg
    16501 actatcacaa aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc
    16561 cggcgtggaa tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct
    16621 actgcattag atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt
    16681 gatcgtgaca aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga
    16741 gtacgctcaa cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacac
    16801 gtgattttca tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga
    16861 gtatgaacat ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt
    16921 caatcacaac tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg
    16981 ttcagaaagt tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac
    17041 aaatgattac caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat
    17101 tcttcagtac actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga
    17161 tattatgtaa tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac
    17221 gtcgaggtgt ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg
    17281 aaaatttgaa aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat
    17341 ttagtgatgc cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc
    17401 ttagtctagg acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc
    17461 acgctcggat tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac
    17521 tgatcctctt acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc
    17581 ttgattctgc tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc
    17641 gcggattaga ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata
    17701 atatagatat gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg
    17761 aattgggaca aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat
    17821 gggaagaaaa aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat
    17881 agctttcaag gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta
    17941 aaaaataatt ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt
    18001 tacagtaatt ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc
    18061 aggcgaaacg atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca
    18121 agcagtggca gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga
    18181 cagcgaagga caacagttat tagatctact gacaaaagaa aacatcgata catctgggat
    18241 agctgaaatc caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga
    18301 aaatagtatc ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa
    18361 aaacaagcaa ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga
    18421 tgcgacgatc ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc
    18481 tgcaccagct cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc
    18541 aaatgaaaca gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt
    18601 cgcagcagcc gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag
    18661 tgccggcgct ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga
    18721 tgccgttgac acaacagccg caggtgatac atttattggc gcattaagca gcacacttca
    18781 gccagacctt agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc
    18841 tgtgcagtct tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc
    18901 agtctaaaat taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc
    18961 agatatttca cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc
    19021 aggcatgcct gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc
    19081 aagtttttta tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt
    19141 ggcggaagaa ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc
    19201 agatacaccg attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca
    19261 tgcatttatt cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt
    19321 ggtcttctaa tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat
    19381 atgctgattg aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact
    19441 cggcttcgta tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa
    19501 atcccttcca ttaagtattt cattttactc gatagatatg cagaaataat catggatcag
    19561 ggattggctc ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa
    19621 aagctaaatc cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt
    19681 ccgctgtttg ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc
    19741 tggcttagtg aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt
    19801 tattttctgc caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat
    19861 attgcggctg tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac
    19921 agtggcaaag cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct
    19981 actgtcatgc cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa
    20041 aaatatattc gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg
    20101 gttcctttga cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct
    20161 gccgcaatta cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt
    20221 ggttggaata ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt
    20281 aatatctctg cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga
    20341 atggctggtg cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc
    20401 tccggttctg cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga
    20461 attgcgatcc cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga
    20521 gctgtaatgg gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca
    20581 ttgcctgcat tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta
    20641 gtcgtagcaa tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa
    20701 tagttaatga aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt
    20761 taggatcatt atttttataa atttttgtga tactttccaa actttggtat aatttacata
    20821 tgcataacca tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa
    20881 atgaacgtca aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag
    20941 aatacacaga cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa
    21001 atgtctaatg aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta
    21061 tgaaaaataa aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt
    21121 tgtcgaccaa taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag
    21181 atacgactac atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa
    21241 ctggaacaac taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc
    21301 catcagaaaa tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg
    21361 gtaagatcca actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta
    21421 cggaaagtgg aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc
    21481 cagctgaagg tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg
    21541 atgcaaagac ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg
    21601 gaccggatac ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag
    21661 atccggaaac gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata
    21721 attcaaacaa taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat
    21781 ctggatcgaa taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat
    21841 cgaatggcgg aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag
    21901 agaatccatc ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc
    21961 ctacaaatac aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa
    22021 aaccatatgt atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt
    22081 acgtatatat gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg
    22141 caggtacgca gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt
    22201 cttccggtgt tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc
    22261 ctcaaccagg agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg
    22321 tccgagtaaa cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta
    22381 ttttagatgg attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct
    22441 tgaaaaagat tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag
    22501 tcatgatggc tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac
    22561 caaatcataa cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata
    22621 cacttgaaca agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc
    22681 cttcttacaa ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca
    22741 atcctgattt ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca
    22801 aatatttgga aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca
    22861 tcgaagcgta cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag
    22921 aagcaaacgc tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa
    22981 cagatggcgt gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt
    23041 cttccatctt ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga
    23101 taccagaaac aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa
    23161 gtttagacaa atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg
    23221 gatgtgacac tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat
    23281 tccgttacaa actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt
    23341 cgctccgctc ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta
    23401 ttcccgcgca tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa
    23461 gcatctgttt ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt
    23521 agtaaaccag attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat
    23581 tggcgaaaat cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct
    23641 gtgccactta ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat
    23701 aatcggctat tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa
    23761 ttgaaataga catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc
    23821 gtcactcgcc atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca
    23881 ggaaatccgt tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca
    23941 ccattgatga ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa
    24001 caatgaccag tgtccgggtc tgtttttact tcgaacgttt catcatctaa tttgaatgtg
    24061 ccattcgtaa tccgtccagc catacgtccg attgaagcac cgatataagg gtctttttct
    24121 agatattctt ttgcttcatc aaaacctaag atcaactctc gtttttcatt atcgattatt
    24181 gtttgaaaat gaacaacccg tgccccaaga tcactgacag acaaagatgt cccattttga
    24241 ttcgtgatcg taatcaagtg atagttttcg ccaaaatttt gttcattgat ttccatgttc
    24301 cttgcaccct cttttctttc atttgcactg attccagcat acatggaaac cgttttctta
    24361 acagcttatt ccgtgagtaa cgagaaaaaa tttactatat tctaataaaa gaccgtgaaa
    24421 aaaaggttgt gacaaaaata ttgtcacaac ctctttttcc gaacaaacgg tgttcaaaag
    24481 cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gaggagctat ttttgcttgg
    24541 ctgttcttat tacttgatgc aggacagctt tttcacaatc tctttttcaa tacgattcca
    24601 gtatataacc atgtcgtcag tacatagtag atgaaataca atacgaagaa tatcgtaaac
    24661 ggaacccaaa tatctttgta tggattttgc atgaatagac caaacatttg taatccgaat
    24721 aagacatgga ctacacctaa gattcctggt gctaagaata acacgcctac ttctctacgg
    24781 atcgattgtt taagcaaccc gcgtcgtgtt cctatctttt caagcatttc ataacgagca
    24841 atgtcactct tgcttcctga caagatcttg aacatcaaac agctagcaag catggtcaag
    24901 aaagctaaac ctaggaagaa tcccataaat tcaaaaccag agaatagacc attatacact
    24961 tcatacattt catatttctg gttgctgtac tctacttttt gcaaagatgg attgttcatt
    25021 ttattttgag caacaagtgt ttcgattggt ttcaggtttg cttggaagtc ttttacctga
    25081 actagctgta aagtggattg tggcaagttc agttgatcaa attctgattg actgactacc
    25141 ttgatttctt tttcttgctg ttccggcaat tcatagcttc gaagttcctc ttgtacagtc
    25201 agttcattca tttttttagc aggaactttt tctttcgtga tcttcggcgg tgttccatct
    25261 tcatgtttta tcatgatcaa tggttgagcc gcaaattcac ttgcattata gtaaatcgtt
    25321 cccgcatctt ctttttgagt ataagaagca gaaagtgttg gtgaaagttc tttgattttt
    25381 tgctgatctt ctgcccgagg attattcatc actagatcat atccagaatt gttttctgtc
    25441 aagtctgcaa tttcattttt aaaacctaat cctaccgtca aagcgcccag tgccaaggca
    25501 aacagcatag cgaccattga gagcatttgt gtatattcac gaatacgaaa gtttaactgt
    25561 gacaatgtga aattgttcaa ttttttcaat gaaatctgtt ctgatttctt tagtaatgat
    25621 aaaaagaaaa tgatcacaga atggaaaatg aaataagttc ctaatacgat cgtaactaag
    25681 gctaccccta taccaaagac ttgataggtc cccacgtgtt tcatcatata gtaaccgcag
    25741 cctagaaaag caattcccag tactacttca aggaaaaaca agaatttatg ctgcttgatt
    25801 cttgttggtg tctgatccgc acgaatcaat gctaaaatcg gttttttgac aatcgaaaaa
    25861 gcattgacaa cagcagctaa taaaaacaaa acagcaaaaa acaaaacagt gatcaacaac
    25921 ccgtgaatac taaaaggtgt aaaatgcgaa atattgagat ctaactgatc cactaataat
    25981 cggtggacga tacttgtcaa tcctacaccg acagctgaac ccactaaagt agctgcgatc
    26041 cctactaaga atgtttcaga aaaaatcatc tgtgcgattt ttctgccttt tgctcccagc
    26101 atcataaaca ttgcatagtc tttttgccgc atcgtcatca aaaaggaatt tgcatataaa
    26161 atataaacga atgtgatgat ccctagtaaa acagaaccga atcggaaaat gataactgta
    26221 gccgcaacga tcgtattact tttcaaaaaa gcttcgttgc ttgccattga ttcaaacatg
    26281 taaaaaatag cagatgccat aactaatcct gagaataaca ccaagtagtc tcttaaacgg
    26341 ctcttgatcc cagtaagtga taatttccaa agcatagtca atctccccct actgttccaa
    26401 gtttccaaga atagatagta tttctcgata gaaggaatca cggctttgat ctccacgttt
    26461 cacttcttga tggataccgc catccttgat gaataaaata cgttggcaat aacttgcaga
    26521 aaacggatca tgcgtcacaa gaagaatcga tacatgatct tttgtattca attcgtccat
    26581 cgtatctaaa agatcccggg cacttttgga atccagcgca cctgtaggct catcccctaa
    26641 tagaatcgtt ggttgtgtga tcaatgcacg tgcggcggcg acccgttgtt tttgtccacc
    26701 ggaaatctca gaaggatagc tttccaaaat atgagagatg gataaacgtt ctgcaatttt
    26761 ttggacacgt tgcttgattt cttttggtct gacgccttgt aaagaaagag gaacagcgat
    26821 attttcttgt gctgtcaagc tttctaataa attgaaatct tggaaaataa agccaatctc
    26881 attcgcacga aaatctgcta gttgatttcc ttttaatgtg gtcacatctt tttgattgat
    26941 tcggatatgc ccatctgttg gtttatctaa tgtggataaa atattcaaaa gtgtcgactt
    27001 accagaacca gaagcgccca tgatcccaat aaattcgcct ttttctacat cgaagctgat
    27061 gccgcttaat gcttgcgtct ttttctcatt gttttttccg tatacttttg ttacattctg
    27121 tacttcgact attttgttca tttctttccc taccctttaa ttagtttttc cagtgctgtt
    27181 ttcggcacgt cttttttttc gacttcttca tacgaagtga cacctttatt tttgttccag
    27241 gtgattttta aatatgctcc tttacgaagc ggtcgagtca attgtccctt gaagtccagt
    27301 tctttttcct taccctcttt cgtatagcca ggcaggatat atctatagtt gactgtttct
    27361 tgtccagagt ctgattttga aactgttttt gtaccatctg tcgtaatttg tacatagtat
    27421 tcttctccac ccataactac gtaatctgcg attttcaccc ctgcaagtac cacaacgatg
    27481 aaaacaagta gaccgatcag aagttttttc actttttccc cttctctctt actttatata
    27541 ctgattatag aaaaaagcga acaattctgc cttcgaatca actatcattc ttctttcaat
    27601 tttgtcattt tctttaaaac aactaaaaaa agagttcaag acaataatcc gtcctaaact
    27661 ccttcttcct atcttacaaa aagtatgtca cttacggtct ttcatgtcgc ctgtattgat
    27721 aacaggttgc gttcctttgt ctgtaatgat tgagacaagt aaattattga tcaattggac
    27781 tttccgatca tcggtgaaat tgatttcttg tccatcttcg atttgctcta aggccatttg
    27841 agtgattgtg acagctcctt caacaatggt ttgtcgagca gacaaaatgg cttttgcttg
    27901 ttgacgttgg agcatggcac tagcgatttc tgttgcatag gctaaatggt tcaagcgtgt
    27961 ttcgattact tcgacacctg ctactgctaa acgctcttgc agctcttttt gcaactcgtc
    28021 agacactgcc gtcgtgtttc ctcgaagcgt caaatcattc tcattgaatg tatcatacgg
    28081 gtattggcta gcgatatgcc gaatggctgt ctcactttgg atttctacaa aatcttgata
    28141 atacgctaca tcgaataatg cctttgctgt atcaacgact ttaaagacta ctactgctga
    28201 gatttcgatg gggtttccat ccaaatcatt gacttttaaa acagaactat tgaaattccg
    28261 aactttcaac gaaacagtca ttttttgtgc caaaggtatc gtaaggaaaa agccattttc
    28321 tcgaatcgta cctaaatatt gtccaaaaaa caaaattact tttgcttgat taggactgac
    28381 tacagttgcc gaacttaaca acaataacgt gatcaaccaa agaaaaatac ttaaaaaaag
    28441 tgcccaaact ttcgcttgcc acatacctaa atagaagaaa aacaatccaa ctaaagctaa
    28501 tacgaccaat ccgacaatcc ctgcataacc attgatataa aatgctcttt tttctctcat
    28561 gctacttcct gcgctccttt atccttcaat aaatatctaa tgttactttt ttaatttttt
    28621 tttcgaatac gcgccattac accttctagt tctaactctt tgtcttcagt gatgtaccaa
    28681 ccgcttgcct gtgtccctcc ggctgcctgc ggtaaataat ccaattttgt tcgaacccag
    28741 tgcttttctt ctgtaaaata gtcttctacc aagacctcta gatccatccc tgctgaaaga
    28801 ggcaaatctt cccgaacaga tttaaagata aatcgttcct tcttcccatc atacttcaat
    28861 attcctgtat cgataatcgg taaagaataa taatccagct ctttcttcac cgtcaataaa
    28921 tgtttgaaaa cttcatggta cacttgctct aattggcgac cttctgaatt gttcgggtaa
    28981 ttaaccatca ctttttcttt gtctggatca tagaaggttt ggcttagttg agtgatataa
    29041 gtatgtaatc tttccatttc tgtttttaac ttttctcttt gaccttcttc taccttcata
    29101 tactctttca ccctgttttc tttttagata ttttctttct tattttacta tagttatgtg
    29161 cgttaagcga tgaaaaacag caaagattgg aaggatcttt gctgtttttt cttaaaatat
    29221 ccagttgagt acaggatcaa tctgtttcat tgtcctcttg atccgtcgta atattgatcg
    29281 atgtgtcttt agcagtgatc tttagctttt tctgtccttg ttctaacaaa gtatttgtta
    29341 ttggctggta gtctttttca atataagatt caccagacgt ttggatagta tagccactgt
    29401 tttccatctc atgcaaagat aaattgccac cgtttaggat gacttttcct tctttgctga
    29461 tcaaactgtc ttctagatgg aagttgctgt tttcacctgt gacgatcaag ttatccacat
    29521 tggcctcatc taatcgaagt gagcctaatt catctttcgc agttattgtt cctcctgtaa
    29581 attcattgaa cgatacccag ccatcatgtg tcttagtatt cacttttttg attgatactt
    29641 cgttgatatc aattgcacta ttattccctt cgattgtcaa ttcagataat tcatgatctg
    29701 cagggacaac aatagtgatt tgtgcacttg tgtcttctaa actaatgatt gcacctgttg
    29761 attttttgac tgcattcaca gtcaacacgc catttttctc ctttatttct ggcttttctg
    29821 tcttatcata agtcgttttt atctcaaaat ccaatccgcg tatgaccgaa actgtttgat
    29881 tttctgcatg gatgacgatt ttgtttgcat ccttgatttt ttttgtctca gtaaccatat
    29941 cgatcaaccg aggaccatta tcccaagtca gactcttttg agcgcctaat gtataagttg
    30001 cacctgctag gaggccacca gtaagcatca gaccaatacc ggtaatcaaa aaatgtttca
    30061 atctcaattt atgtgtcctc cttcaaacgc ccgctgaatg cagatctctt tttgtaataa
    30121 aacgtcgtcc catccattta acaaagttca tcatgacata aaaaagccct tttgttcctt
    30181 tcagcaacag tggcgaaata aatactgcgc ttcctgtggc gaacagaccg acaccgataa
    30241 agaataatcc actcagaatc gattgtccaa acacactgat tcctgagatg atcatgaata
    30301 ggccaacacc taagatactg atacataaaa catagatact aaaaatgagc gctagtaaag
    30361 caaccaatag tcccccaata accaatataa accccagtgc tagtggaatg aggacaggag
    30421 aagcaaataa accgataatg attaaccaaa ccaggcgaat ttgtcttttt ggatttttca
    30481 ctaactcgtt ttctgcttgt tcttcttctg aaaagtaact gatttttaat tttaaggcaa
    30541 actgctttgg tgtaccatat tcatcaatga tctgatctaa tgtttttcca gaatctaaaa
    30601 aatattcctc ataatactgg atcaaatcaa aacgatcgtc ttcaggaatt tcttgaagat
    30661 gatccacaac ttcattcaaa tattcattca tttgtgctct cctccaataa ccggtctaaa
    30721 gaatctcgaa aaacaatcca ttcatcaata atttcttcta aatgacgact tcccattggt
    30781 gtcaacgagt aatatcgacg gtttctgcct tcaaaaggtt catcataggt ctcaagatat
    30841 ccttcttttt tcaatcttct caaaaccgga tatagtgtag attcactgac agagagactt
    30901 tcttgtacga tttttgttaa ggcataccca tacatatctt ccttgcttaa tgctgctagc
    30961 acagtaccat caagaagaac agttggaatc tggatgtgca ttacgtcacc ttctattctc
    31021 aagtgtaata ttttatttca atctatatta tacaacatat aatatagttt caaaatcagt
    31081 ttgtcaaata caaaaaaact gactaaaaga tttgatcctt tttagtcagc aaaaaatggc
    31141 ttctctttga tttttattat aacagtggca taatccaatt tgtccacatc cagctcagtg
    31201 gcatgataac gatcattgca ggtatcatgt cagctactgg gaacattttc acgttgatca
    31261 ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc tttaaaatct aagatcatgt
    31321 ctggtgtagt caatggataa ataaattttg ccaataagaa caaaatataa aaaataatga
    31381 attggggaat tgctatcaca gaaacgacat accctaaatt acacgcaaaa atagctgctg
    31441 tgaaaaaatc aaggacagat ttagaaatca aaatcgtact gtctcctgtc atcccagaat
    31501 ctaaacttcc atagatcccc gtaccacttg cacaaaatag aacaatgacc gtcactaatg
    31561 tgctgataaa ttcatcgtgg gtcataccaa gcttttcact agggaagatt ttagaaatag
    31621 gtacttgcat cagcgcagcc cccttattta tcccattacc taaatgaaca atcaaaccaa
    31681 tcgctgttcc tatcactaaa gcaaaaataa cagccggcat atttttcatc ggcgcaatgg
    31741 aatagattcc catccccatc gaacacacac caaaaaccat cgtcagctgt gctttaaaat
    31801 gttcacttaa tttatttcct actaatccgc caattagtcc gccaaacaaa acggaaccta
    31861 cattgatcaa aactccagtt ggcatcaaaa aactcctctc tttttctacg aagcgaaaaa
    31921 aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga aagattgtaa cagacagaga
    31981 agattacacc aattcatttt ttgtgttaaa gtattcttta ttggaataag taggaggaat
    32041 atgatggata tccataaatt cgaagttttt cttgatttag ccgaaacaat gaatttcacc
    32101 aaaactgctg atcgacaatt caccacccaa ggaaatattt ctaaacagat tctttcgtta
    32161 gaaacagaat tagacgtcaa attattcgaa cgcgcccata gaaaaatcga attgaccgaa
    32221 gctggaagct tattgctccc atatgtaaaa aatgtcgtag aacaatacca tgctatgcaa
    32281 gaagtgcttg aaacctatac gaaagacaac aatctttcat taaatatctt aacgataccg
    32341 acaatgatca attataaagg tttttccaaa atcaccgagt ttttgaaacg ccatcctgaa
    32401 tttacagtcc agttgaaaga agtggaaagt gtcgaattaa cttacaatga cgccaatgaa
    32461 catagtgaca cgatccactt tgctagaagt ttccaggccc caccaagttc gattgagtgg
    32521 ttgcctactg aaacagacga ttttgtagca gttctaccta aaaatcatcc tttagctagt
    32581 aaaaagaaat tggatctaag cgaactgaaa aaggagaact ttttgctcct tggccccact
    32641 acgaacttgt atcaacctgt actagatctg tgtcgcgaag ctggatttga accaaaaatc
    32701 tcttataaag gagcaagaat cgacttgatc atcaatatga ttgcaaatga catggggatt
    32761 gctattgtta tggaaaaaac agtcaagaat ctattaaaag aaaatactgt gattctccct
    32821 atttctccaa caacagagag ttatcttgca tttactcgta aagtcggtga gcattctttg
    32881 gcttcagata cattttggga gtatttaaat caagaagaaa actaagccct acattttctg
    32941 tccatctatc cacccaaaaa aagagagcga cgcatgactt tcgctctctt tcttgcatca
    33001 tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt caataagaaa gcagcgattt
    33061 ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa aaaaacgact aaaaacccct
    33121 aaaaaccttc aatagaattt ttaggggttt tattcttttt cctattgttt acttaaccgt
    33181 atcattgctg tcgttcctac tccttcgata ctgctataat caatacgtcc atcatgcagt
    33241 tctagaattt ttttaaccag tgaaagtcct agcccattcc cagaaatttg acgacttgta
    33301 tctccttgat aaaatttttc aaacattcga tctctggtct cttcattcat tcctacacct
    33361 gaatcggtca cttccagtac aatctctgtg gctgtctcaa atagtttgat atgaatctgt
    33421 ccattgattt gattgtactt tatggcgtta tccatgatat tcagccacac ttggtacaaa
    33481 aactcttcat tgcctgtgta atttactgca gcaagttcga tatccaattc tagctgttct
    33541 ttctcccatt taggctgaaa aaaaacgatt acttctctga tttgttcatc taatcgatac
    33601 tctttctttt ccagttggat acgctggttt tccagtttgt tcaattttaa gatgttctct
    33661 gttaattgtg aaagctgcgt gatactttcg ataatccgat ataaaaatat ttggcgttct
    33721 tcgtcagaga gatttggggc ttgtaacaat tgaacatatc cttggattgt tgctagtggt
    33781 gttttgaatt catgggatac actggacaca aagtcattcc tcagtgtctc gatactattg
    33841 agttcttgga ccataacgtt gaaatcttta tacagttgct gtacttctgc tactttctgc
    33901 tgttcatcca ttcgaatcga aaaatctccc gttgctacca gactcatatt tgtccctagc
    33961 gcactgattg gatgcaggat acgccgccca acaaaaatcg atattcctgt acccacgacc
    34021 atactgaacc ccgcaaagac gaataacggg tataacgttg gttttccagc cgattcagca
    34081 ggcgagatag aatgtcgaga caataagaaa ataaaaccga gaaaacacag cagtgtcact
    34141 aagatcgaaa tgaagacaat actagcaaaa tagatccata attgtgaaaa tagctttttt
    34201 ttcatcttta aataaccgcc ttatagccta gtccccgtac tgtgatgatt tgaaaatctt
    34261 ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac atcgatcgtt cgttcttctg
    34321 tattcgtatc taatccccaa atatcatcca tcaactgttg gcgagtgaag atcttgttgg
    34381 gataagacag taatttatac agaagcagaa attctttttg aggcagaatc aacgaatgct
    34441 gatcttgtaa cactgtataa gcatctagct ctaattttgt ttgtccgatt atcaagctac
    34501 tgctgtaatt gatctttgaa cgtcgaagca atgcttccac acgtaggatc atctcattga
    34561 catcgatggg ttttgtcata taatcatctg ctcctaattt gaacccgcgt ttcttatcct
    34621 caaaatcggc ctttgccgta atcattagaa caggtgtgtc aaatctggtg ctacgtaatg
    34681 agtctagcaa gtcatagcca tccattttcg gcatcatgat atctgtaatg attaattcta
    34741 tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt caatgcggtg atgactgaaa
    34801 agcccgctcg ttccaacaca gaacggtaaa gcaactgtaa ttgcggatca tcttctacta
    34861 ctaaaatacg attcattgat ctccctctgt tttttgaaaa atttctagat tttttcttgt
    34921 agttcatctt cagttaacat ttatttcgta tactacgatc attgttctga tatacttatt
    34981 agtataccga atatttcaat acgggagaag ggtgattatg cttcaattaa aagatataaa
    35041 aaaatattat aaagtcggcg aaaccaccac caaagcattg gatggtgtgt ctgtcgcttt
    35101 cagacaaaaa gaattcgtag cgattttagg tcctagtggt tctggtaaaa cgactatgct
    35161 gaatgtgatt ggtggactag ataattatga ttccggtgat atggtaatca acggaaagtc
    35221 tactaaagac tttaaggaca gtgactggga tgcctaccga aacaactcta tcggtttcat
    35281 ttttcaaagt tacaacttga ttggtcatct aggcattatt gaaaacgttg aacttgggat
    35341 gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg gcagaagatg cattgcaccg
    35401 agtcggcttg acaaatcata tgcataaaaa gccaaatcag ttatctggtg gacaaatgca
    35461 gcgagtggcc atcgctcgag cactagccaa tgacccagat atccttttgt gcgatgaacc
    35521 aaccggagcg ttagatacag aaacaagtat ccagatcatg gaattgatcc aagagttatc
    35581 gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa ctggcccatc aatacgcaga
    35641 tcgaatcatt gaattttctg atggtaagat cttaacagat tcccatcctc acatcgaacg
    35701 accaaaagat gatcaattta acttacgcag aacaaagatg agtttttgga cagcgttaaa
    35761 gctttccttc aacaatatcc gaacaaaaaa aggacgtact ttcttgacct cctttgcttc
    35821 cagtattggg atcatcggaa tcgcgatcgt tctgtccctt tcttctggat tccaaaaaca
    35881 aatcgataac acgcaagctg aaacgatggc gaaatttccg attactattt ctaaggtaac
    35941 gactaaccag actcgtgacg atgccggatt gggtgcaagc aaagcggatt atccagacag
    36001 caaaacgatt actgcaaaag tcagcgaaga agataaagca cagcacacga ataagattga
    36061 tcaaacatac gtcgattacg taacagacat cgatcctaac ttaagtaata atatcggatt
    36121 tacccgtact acaggaatca atcttttacg tgatgtaaat ggcaaagtac agcctgtcag
    36181 cttctcgaat caaaatcctg atacggaatc tctttcttta tctagtacga tgtcagcaat
    36241 gacaggtgtg ggagtgtcaa gtttccctac acagcttgac actagcaaag aaaacttttt
    36301 aaaagacaat tattctttac ttgccggaag ttatccagcc tcagcaacag atgtcgtgct
    36361 gatcgtagac ggcaataata atactaatat caacgcattg aagaatcttg gatttgatgt
    36421 gaaagaagac gaaaaacttg attttgatga tatcgttggt acaacattca aattagtaaa
    36481 caacaatact tactatacaa aacttccaac tgggaatttt atccctaata cagattatga
    36541 tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg gggatcctac gcgtgaaatc
    36601 ttcttctaca atgaatctct tatctccagg aatcgcttat agcgatcaat taacgactca
    36661 aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct caaagagaca gtggtgtaaa
    36721 tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa acattgctta gttatttagg
    36781 tggagacagc ttgccttcaa gtatcatgat ttatccgaac aattttgaag acaaagaaaa
    36841 gatcttggat tatctagatg attacaataa aggcaaatca gatgaagata aaatcattta
    36901 tactgattta gctggaacga tgactgaact gactggcgga ttgatggatg ctatcactta
    36961 tgtccttatt gcttttgcag ggatctcttt agtaacaagt atgatcatga tcagtatcat
    37021 tacttataca tctgtgattg aacggacaaa agaaatcggt gtactaaaag ctttaggtgc
    37081 acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg tgtattttag gtatttcttc
    37141 tgggatatta ggtgtgttta ttgcatggct agctacgttc cctatcaata gcatcttata
    37201 caacatgacg gatctaaaaa atgttgctca actaaatcct gtccatgcga ttattctagt
    37261 gatcgtctct actatattga cgatgcttgg cggtcatctt cctgcacgaa tggcagcgaa
    37321 aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt gacaaccaca tttagatttg
    37381 agtcataaga gcgctgaaat agtagaaaac agcaagaaac gaggaagtaa tatgttcctt
    37441 gtttcgcgct gtttttttta attatttttt ataattctgg tatacttttc tttcctttgt
    37501 accacaactc ttctggcgta tacctgtctt ttccatttat gaatactgtt ccatctgctt
    37561 caatcgttac ttcagtccca atccacatat tacgagaaag tgcgagaaga ttttttcttt
    37621 ttcctcttcc tttttttcca aattgcaagg catcggttat ctcttctgtc caatgagatt
    37681 tcacgtattt tacttctccg ttttcaaatg taactttcaa aatattcgct atattttcca
    37741 cttttactgc tttaggaaat gactcctcca tttttttcac tttccttcct ccaacactaa
    37801 gtatgcgctt tcttttagtg ataagtatat catagtttat tctattatca aaaaaacgca
    37861 tccattccat tgtagaatac tttaccgaac aagttgaatt gttttcctct ttttcacctt
    37921 ttactataga taggtaaaga caactaagga ggaatctctc atgacagatc aaccgtatga
    37981 tttaagaaaa gtcatcgaag aactaaaaac acttccaggt caatatcacg aaacagatat
    38041 tgaaatcgat cctgaagcag aattatctgg tgtttaccgt tatatcggtg ctggcggtac
    38101 agtaaaacgt ccgactcaag aaggaccaac tatgactttc aacaatatca aaggtttccc
    38161 cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg aaattttgca
    38221 ccatgattat cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc caatcaaacc
    38281 tgtgaaagta gacaaagcag acgctccagc acaagaagtc gttcatctag ctaccgatgc
    38341 agacttcgat attcgcaaca ttttgcctgc acctacaaat actcctgaag atgctggtcc
    38401 atacatcaca atgggtgtag tcttaggatc agatccagaa aaaacaatga gcgatgtaac
    38461 gatccatcgt atggttctag aagataaaga tacgatcggt atgtacatca tgcctggcgg
    38521 acgccacatt ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa tgcctatcac
    38581 tatcaatatt ggtttagatc cagcaattgc cattggcacg actttcgaac cacctaccac
    38641 tccacttggc tacaatgaac tatggtgtgc aggagctttg cgtaacgaac ctattcaaat
    38701 cgttgatggt atagcagtcg atgaagtggg tattgctcgt gctgaattca tcattgaagc
    38761 agaaatcatg ccaaatgaaa caatgcaaga agatatcaac acgaactcag gaaaagcaat
    38821 gccagaattt cctggctata ctggggatgc taatccagct gtacaagtag tgaaagtcaa
    38881 agcagttact caccgaaaag acaatccaat catgcaaaca acgattggtc ccagtgaaga
    38941 acacgtatct atggccggta ttcctacaga agcaagtatt cttgccttgg tcgataaagc
    39001 tattccagga aaagtagtaa atgtctacaa tcctcctgca ggtggtggta aattgatgac
    39061 catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc aagcagctat
    39121 tcttgcttta tcagccttca aagaattgaa gacagtcatt ttagtcgatg aagatgttga
    39181 tattttcgac atgaacgatg tagtttggac tttcaacact cgtttccaag gagataaaga
    39241 tatcatcgtg atcccaggaa tgaggaacca tccattggac ccatcagaat taccagaata
    39301 taatcctggc tctattcgtt tccgtgggat aagtgcaaaa actatctttg acggtacagt
    39361 tcctttcgat atgaaagaac attttgtacg tgcaaaattc caagaagtag aagattggga
    39421 aaaatatttg aaataatgaa tgatctgcaa ctgttttgac atcataaaaa ggagtgcgga
    39481 aaatggaaca attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt ggcgcttcag
    39541 gcaccattta tgccatcaac ctagtgaaga aactaaaaga atatccgatg atagaaacac
    39601 acggaatcat cagtccatgg gcaaaacaga acttaaagct ggaatccgac ttatcactaa
    39661 ctgaattcga aagcctgttt gattaccatt attcaaacaa agatttaggt gctgctgtcg
    39721 ccagcggatc atttttgacc gatggaatga tcatcgtacc ggcaagcatg aaaacagtcg
    39781 caggtatctc tgttggttta ggcgacaacc tgattgcacg tgctgctgat gtaacaatga
    39841 aggagcaacg aaagttagtc attgttcctc gtgaaacgcc attaaatgcc attcacttgg
    39901 aaaacttgac taaattagca cgactaggtg tgcaaatcat accaccaatt cctgcttttt
    39961 ataatcagcc tcaaacgatc caagatcttg ttaatcacca tacaatgaaa cttttggatc
    40021 aatttgaaat tgtaaatgag cattcaaaac gttggcaggg tgattaatat ggaaaaagaa
    40081 acgatagttg aagtaaccaa actaggtttc acgatccaag ctgctgttaa agtaatcggt
    40141 aaagacttat tgatcacttt aacaggcgga gatacaccac atatcggaac agtcactaca
    40201 ttttctcgtg atacagagat gcaaactgta cgtttcccta gccacgatgg tcggttccat
    40261 aaagacgatg tcttagccga aaaaatagct gtgatcatca aaactgccgt tccgggaagt
    40321 tgtacgatca cttccggtat ccatgttgac catatctcca atgcacaaat caacgcctct
    40381 tttccgatgt cagaagagtt aggttacaag attttagatt ggttggaaaa aatagatttc
    40441 catatcaaag aaccagttta ttataaagat ggtgaaaaac cattatgaaa gaagaggttg
    40501 tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc ttctcatatt
    40561 ttgccgtttt tgagctaata ccgagggaca gctgattgaa cgccgtttat caagaggttg
    40621 tgataaagcc gcttcgtttc gagtagtaag aaaaattttg taaacatggg tttccacatt
    40681 tttgcataaa tttgacttac taccgagaaa ctggcttttg aacaccgttt atcaagaggt
    40741 tgtgacacgt cttttgtcac aacctctttt atttttgctt cactgtaact gctacaatat
    40801 caccgatttc ttttccgatg tttttacgaa tatcttttcg tagtccaagg atgtagcaga
    40861 ctgaaccatc tggatttttc acgcccatat tcacgatact gccttcatat ggcacattgt
    40921 caaaagttgc ttggacttta actcttccag caccgaattc ttttcttata tcatacggaa
    40981 aaataatata tgccccgccc ttgccgactt cggaagcata gatttttgat tcaaaagaat
    41041 aaatttccct cattttattt ctcctatttt ttacatctag tatagcatag caaaaaaaag
    41101 aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg tgttctcctg
    41161 ccaactcctc ggtcacaagt cacaatagtc aacaaacatg agaagctgtt ttttgaaatt
    41221 gctccttgtg actcagagtt aaacggcagg ctcacaacct cttccttaaa cggtgttcaa
    41281 aagcttgcat ctacggcact aagcccaatc agatgaaaaa tatggagtgc tatttttatc
    41341 tgattgttct tactgcttga tgcagagcag ctttttcaca acctcctaca tggtgttcaa
    41401 aatattattc gccttttgct tctttcacta attttgcgaa tgcttgttgt tggaattcag
    41461 cagctaaggc tttcgtacgt tgttcttgct ctgctttgct catttttgga ttttcagtca
    41521 ttgcttttac cataccgtct tgaacataag cttttgcttc actttccatc gtactttgct
    41581 gtttcttcac taattcagct aacttggctg cttggtccgt acttaattct acccatgtct
    41641 ctgggatttt gaatgtattc actcggctgt catattcgtg attgttatca gcaatattga
    41701 accagaattt atacatgtcg tttttgtatt cccaataagt agttttcgtc actttttcag
    41761 catcgccgtc tgtcttttct actttatttg tttcgttatc cgtaccagtt gcttttggtt
    41821 tttcttgttt ttcatttgtt cggtaaagat acactttttc ttttccattc cctaaagctt
    41881 tatacaaaag catatctgct ccttgggaat cagcactgga aacaattttt tctgtttttg
    41941 tttccgttac tttttccatt ccaaagtgat tggacaaatt agcgacgatc aaacttagtg
    42001 aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt tttttagcga
    42061 acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa aactccaata atcatgcatt
    42121 atcccccgct ttcattagaa caggttcttt tttcttcaat aaaaaggcaa taataaagcc
    42181 aagaatccca aagatgatcg ctacgataaa cgcggcatgg taacctgaca gtgtggcatt
    42241 gattgcctga tctttataag ctaatggcgt attatccaat acagatttgc ttgggatatt
    42301 tgcttttgtc acattcgtca acacactgat caaaatagct gtaccaatcg agcttgcgat
    42361 ttgacggaat gtattattta ccgctgtccc atgactgatc aagttatttg gcaatgcatt
    42421 cataccagaa gtcgttaccg gcatcatgac catggagatc ccaaacatac ggatcgcata
    42481 aaggatcacg atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg gtgcagttgc
    42541 cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt tatcgaaaat
    42601 cgcacccgtc acaggcatca tagcccccat gatcaatgca cccggcaaca acatcaagcc
    42661 agaatggaat gctgattctc cacgaatatt ttgaatatac aatggtacga ccatttctgc
    42721 ccctaccatc gccatatttg ttacaccagc taaaacagct gcaatcgtga atgtagatga
    42781 tttgaataca cgaagttcca agaatggatg ttccaaatgt aattgacgcc atgagaacaa
    42841 ggcgatgaaa atcacaccaa caactaagaa accaattact ttcaagcttc cccagccgtc
    42901 atctcctaca ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg ataaaatagc
    42961 ggataaaata tcaatgctag gatttgacaa tggaataaca cttttcatta ggaagaatgc
    43021 taaaaccaag acaagaacaa cgattggcag taccatacca aacaagtcac gccaatggta
    43081 gttatcaatg atataaccag ataatgtagg gcctaaagca ggagcaagtc caataacgat
    43141 ccctgacatc cccattgctg atcctctttt ttctggcggg aaaatagata gcatgatatt
    43201 ttgcaataat ggcatcgata ctcccacacc gcaagcttgg atcaaacgac cgattagtaa
    43261 tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga tcgtcatggc
    43321 agaaagatat aaatttcttg aactgaattt attgatcagc catgctgtta ttgggatcat
    43381 gatcccgttg actagcaaga aaccagtcgt taaccattgc acatcagacg ctgaaatatc
    43441 gaaagctttc atcaatgtag gaaaagctgt cgtcaataag gtctgattca aaacggtaca
    43501 aaatgtacca attaataaga caacaactaa aagtgtccga ttatatggtt ttccgtaaat
    43561 atctacgggt tggttgttac tcatatttct ccttctttct gaattttttc tgaaactact
    43621 agaatacttt agtcctaaat tcgctatttg tcaacctact tgacacataa gttaatacgg
    43681 atataattaa gggaagcaag aggacaaaag gagtgacaaa aatggttggg gcttccctta
    43741 aacgtttgtt ggaaaatgat caattggttg ttggcatcag tgaattaagt gatatcgtag
    43801 gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg attcattcgt tcgatcgaag
    43861 ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt cataaaagta gaactgatca
    43921 aaaaatattt agatgacgga tttactttgt caaaagctgc agaaaaggca gaaatccgcc
    43981 aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat atcgaattga
    44041 tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa agaagagaat gctaagatgt
    44101 acatcattca tgacaacgag acagacgaat taacttacaa gctccttcct gtcgatgaaa
    44161 aagctgacta cgaacagttg ttaaaagaac tctaaacgcc actaatctcc taagtaccaa
    44221 gctttttaat aagtagatgc tacataaata cttgaaaaaa ggcctgtttg aacataagaa
    44281 tatccgaacg gtgatctgcg tttctttcat atagaaagga attctccatc actgttcgga
    44341 tattttttta tttttttcgg ttactttctt ctaatacatc tgttagccaa tttgcatatt
    44401 ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt tcatgttttt
    44461 ctttttcagt aggaaagatc gttttttttc gctctaatag atgaagtcgt ttttcttcat
    44521 gaaggtcacg ctgctgctga ataatttcta ctaatgccgg atcattcacg tctttgacga
    44581 aatacaattt cagaacaaat tcgtctcgat taaccggtag ttcattggtg ggtgtatgaa
    44641 tccactctgc caattgttcc ttcccttctt ctgttaattc ataaactttc ttttccaatt
    44701 tttcacctgt gatctctact tgatgctgaa tgattccttg ctcttccatt tttgcaagtt
    44761 caggataaat ctgactatgt tttgcttgcc aaaattctcc tatttcattt ttgaacgatt
    44821 gattcaattc atatcctgtc atcttcttct tacttaataa ccctaacaaa atatatcgaa
    44881 gtgtattttt tcttgccatc agttttccct ccttgacaaa tctttaaatc cattctatca
    44941 taaatgagta atcgaaaaca tgtagtttat tacatgtttt aatttataag gagatgctta
    45001 aacatgaaag aatttaaaga attggaagat tttattggaa ctcactttat ctatacgtat
    45061 gacaatggtt gggaatatga atggtatgcc aaaaatgacc atactgttga ttaccgtatc
    45121 catggcggaa tggtcaaagg ccgttgggta aaagaccaag aagctgatat cgttaaattg
    45181 acggacggcg tattcaaaat cacttggaca gagccaacag ggacagacgt ggcattggac
    45241 tttatgccaa atgaaaagaa acttcacgga atgatctttt tccctaaatg ggttgaagaa
    45301 catccagaaa tcactgttac tttccaaaac gaacatatcg cagaaatgga agcagctcgt
    45361 gaaaaatatg aaacttatcc aaaattactt gttcctgaat ttgctacgat cacttatatg
    45421 ggtgatgcag gtatagacaa tgaagatgtt attagcgaag aaccatacga tggaatgcca
    45481 gatgatatcc gtagcggaaa atacttcgat gaaaattata aacgagtaaa ataaggttgt
    45541 gaaagagagg ttgtgaaatc agcgttctga cctgagtatt agagaaagaa acggaaaaat
    45601 agttcctcat gttttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt
    45661 ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa taagaacgct agttagaaaa
    45721 tagcttttca tattttctag gctagtgggc ttattaccga gaagcagccg tttgaacacc
    45781 gtttatcaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaatggaaa
    45841 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
    45901 cgtttatcaa agattgtaac aactgcatat tcacacagtt aaattagtga cttcaaaatg
    45961 aaataatgct cccagcaaaa aattaggctg ggagcattat ttcgttattc tgttcatcaa
    46021 aaatcagcgt tttgattcca tttcatcaca agttcctcct catttactgc ttcatctttt
    46081 tgctgtgttt gaattgtaaa accttgtgaa agatagaaat gaatagctga ttgatttttt
    46141 tgatagactg tcaaagtcag tgttttattg gtttctttta tttttttcat tagttgcgta
    46201 ccgattcttt gttgccggta ttcccttttc acaaagattc ctgcgataaa ctgatcaatc
    46261 accccagcaa aaccaataat ttcttgatct atttctgcta agtaaatcac tgcatctggt
    46321 aaagctttcc taacagattc tacttgattc tcccaataag ttgatggaat gaattcatga
    46381 gaatccaagt tagcatgcag ccaaatcgat agaacagatt ccagttcttt ggcagacaat
    46441 tttgttgttg tgcggatcat tttttctctc cttacttatt cattgatttt aaaataaaaa
    46501 ccattttttt gaagctcttt tacaagtttt cgcccctcaa aaaagtatat cttctcttgt
    46561 tttacttttc taataccaaa agaaacagca actttaggat catcagctga aacgattcga
    46621 taagttccac caggcaattg atccagcttg actttcccat gatggtcgct ttgtactgaa
    46681 aacggcacac catttctagt aagtgttttt ttcttatgtt tcaaaatgaa tgaactacca
    46741 tttaccggtt tccctgacga atcactaata ataaatgaaa aatcaagcac ttttttactt
    46801 aatctatact gatagattag tcgtccgata aacactaaca gtatgactac agctattatc
    46861 gttcctgcaa gaataaacca ttgccgcatg gttctttcct gatttccttt tccttcttct
    46921 ttttttaccg attcgttata aggaatccga tgtcctgtca ctagcaaacg atgcgtgttg
    46981 atcatatacg gcgtacatgt aagcaaggta actaagtctt ctccttctga aatcgttaga
    47041 acagacgtgt catctggcag taccacttgt atatttttaa cttgataagc caatttctcc
    47101 cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac gatctaattc ggtaaataac
    47161 cgtcgattag gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt tccaccaagc
    47221 gggaaagagg ttccttgcaa gacgcctgct ccattttcaa ggatcctacc attgagccga
    47281 tcataaagtg aaatatttaa ttggatctta ggtatattga tacttccgat cagccattcc
    47341 tctaattggc tggatgttgt actttcattt ctgttctttt cattgaaagg atcagtacca
    47401 ggattgaatc cttggtcagt taatcgttgg tttttctcct ccattttctt tcgctgcact
    47461 tcaatttcag ctgcggtccg tttttgtact tgttcgatac gtttttgatc aataaaacta
    47521 tttaaagaat ccacataaaa tggatataaa gcgatcagta accctacgac gatacatatt
    47581 aataaaaaca aacgaaacaa aagtcgacga gactgttttt gcttactctg cttcacatgt
    47641 gtcctccctt gcaagttttt ttgttctttc ttttattata tgggttcagg aaaaaaatag
    47701 aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata agtacagccc
    47761 aaaaaaatag ctactcatat tttttgttgg gttgggctta ttaccgcaga tgcaagcttt
    47821 tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat gctttgagta ataagagcgc
    47881 tagtcagaaa atagcttctc atattttcta ggctagtgag cttattaccg agaagcagct
    47941 gattgaacac cgtttaccga gaggtcatga aaaagctact ttgtttcgag tattaagaaa
    48001 aattctgttt caaaacaagg agatgcaaca aaacttatgt cgcatcctct ctccttagaa
    48061 ctttcactta tgtttttctt gtaaaattca taaatatttt catgaatttt ctaatttctt
    48121 gcctacctct cgcataggat atatgatgaa tttatctaaa gtttgcaaag gagttcgaca
    48181 attgaaaaaa tatggcccat ttttagcaat ttttttcatt gctttggcat ttctcatgcc
    48241 tcatttattc acacataaac taattttggg tgctgatgcc ccattccatt acaatcggtt
    48301 ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat tattttcctt ccctttatgg
    48361 attccagcaa agtggcagga tcgtgaatgc cgtttacggc ccatttttcg cttatttaca
    48421 aggattgatc gtgttgcttg caggtagctg gtttcattat cagattcttt caaatttcat
    48481 tctttatctt atttcaggat tttctatgct gattttactg agatatgtca atgttcgaaa
    48541 ttgggtaagt gtaccagtag ccgttttatt catgagtacg tattctattc aatactggtc
    48601 tatcaaccaa ggattcacta gctgggggac ttgtttttat cctttatgta tgatccctat
    48661 tgctgatttc gtccttcata aaaagttccc tgttatcaaa gtcgctgtct cagtgggact
    48721 tatgacacag atccatttac tgtcttcgat catgttagct ttgatgtata tcccttttta
    48781 tctttattac ttttttaacc agactgaaaa aggaaaagca ctgcttcaac tgattaagtc
    48841 ggtcttgctt tattttgttc tgacagcaaa tatttgggca ggattagcta ttgtttacag
    48901 tgggaataag attcttcctc cctttgttaa cagagaaatg agtcaaaagg cgatagatct
    48961 ggatggcagt tattggctcc attaccctaa ggcctttcct atattgatat tatcaggttt
    49021 tttgcttttt attttctttc aacaaaagaa caccttattc caaaaaatcg tgttgtacac
    49081 aagtcttgtt ttcttttttc tttctactag cttgattcca tggacgacat tgatcgaaaa
    49141 aaatgtgcct tttattagtt tgatccagtt tccatttcga tttttcgtgc catttacgct
    49201 attgcttcta ctttaccttg ccctttcact gaaccagtgg tctgaacaaa agtggttccg
    49261 tcttttaagt atatttggca ttcttatttt ttcagcacag acgatccaga acttatacca
    49321 gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga catacttatc tattcgatac
    49381 gccggaagaa gcaaggaaaa cattcttcag caaagatatg tctaccagct tgttcgtttt
    49441 ccaaaaaacc acacctgact atctgccaat ctataaggag acaaaagaca ataaatacga
    49501 ccggtataac gaagaaatac tagaaaacga aacaactttt ataaaaacac atccaggcgg
    49561 tcttttgaca atcaagtgga aaaacactgg ggacaaaact gttcatatcc ctgttatcgt
    49621 ctacgatcgg actgtgctac agcaaaatgg aaaaacattg actgattatg aagtaacaga
    49681 tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc ttcattatca
    49741 aacgccaatc tatttctatt tcattctttc tcttactttg attggctggt ttaccttgct
    49801 atgtttattt atttatcata gatacaagct tttacgtgct taggcaaaag ggataaatac
    49861 atccgatcaa aaaactctcc aaacggtata atcgtttgga gagttttatt ttaatcagtt
    49921 tttttatgtt atatgttata ttttttattc gttcacatct aattcgattt ccaccccgta
    49981 gtgatcactg atgattggac tatcgatccc atcaaaaatc cgttgatatt ttttaacctg
    50041 cattccttca gggacgaaaa taaaatctat acgcaatttt tctgtatttt gttcccaacc
    50101 atctattttc ttttccacag tagctgcttt tcctttttca ttcgcgacaa catacgaatc
    50161 ttgaagaaga aggcaacttt ctcttacagt ataataacct tgagagtcta ctggattatt
    50221 gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt ccaagttttt
    50281 ccactcaaaa taaaagccac tttttttatc tgtccaccaa gaaaagtggc agctcgagac
    50341 aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag atcttgcgtg tctgataatc
    50401 ttctttcttt tgaaattggg agacggtaag gacctgactt tccaatgttt ttttcgtaag
    50461 cagtgcattt ccttcttcat atttatcata accgatgtgg ctcatttccc aactccaaaa
    50521 atactctttt ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat tatctttacg
    50581 gataggtact tgattcttta ccggacagaa cttcattagc ttcgcaggtt ctacttcttc
    50641 agattccagt aattggttga tttcttgtaa agcaatcact tcataatctt ctaacaggat
    50701 cttatctgct aattcttgca attttttctc tggttctggc tcaagccagc tatgcgtgtt
    50761 gagtgtcatg aatttcattg gttttctcct tgtcttcatt tcgaaacaaa agggttgtga
    50821 caaaattttt gtcacaacct ctccagcaaa tagtgttctc ctgctaactc ctcagtaata
    50881 agctcaatgg accaggagct attttttgtt tggctgtttt tatcacttgg tgcagggcag
    50941 ctttttcacg acctctccac taaacagtat tcaaactgct gcttctcggt aataagccca
    51001 accaaacgaa aaataggagg agctattttt gtttggctgt tcttatgact tgatgcagga
    51061 cagctttttc acaacctctc cttacatttt aatggagccg atcactttac cagctgtcac
    51121 tgtacctaat tgcttgatca ctacttcttc tactttatcg tttgtaaata cgatcatgat
    51181 cgttggatct ttttcttcgt tttggatcac atcaagatcc atctcagcta atttcgttcc
    51241 tgttcctact tcttggcctt ctttgacagc aatctcaaaa gctggctgat tcatttggac
    51301 tgtatcgatt cccatatgga tcaaaacttc gataccatct ttcgtttcca gcccaattgc
    51361 atgtttgcta gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa cttctcttga
    51421 tgttggaata acagcaaagc cgtcacccat cattttttgt gagaataccg gatcttttac
    51481 ttcagataaa ggaatcactt ctccagaagc tgcggaaact agttcttgtt ctatacctaa
    51541 gtattgctta ctacctgctt gtccatttcc tgtagaaacc atttctggct ctggaatctc
    51601 tgctcctgat tgcagcagat cttcgatatc tgatttcaag acatctgctt ttggtccata
    51661 aactgcttgg actccattgt ctttgacgat caatcccatc gcccctgctc gtttccaggc
    51721 ttcttcagtc cctacttttt ctcgatcatt tacacttacg cgtaaccgag tcatacaagc
    51781 atcgacatct ttgatatttt cttttcctcc cagcaaatga atgatctgga ccacttgctg
    51841 atctgctgtt cctgttccgc tgttagatac aacggtttct tcactgtcat tgtcgtaatt
    51901 tccgttacgt cccggtgtag caaagttgaa ttttttgatc aagaaatttg ctaagaaata
    51961 cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg ataagatctc cacctagacc
    52021 cgctttgata gctaacggtg tccgagtcag caattcgata ttcccaaatg aatggacccg
    52081 caatggcaag atatctgcca tcgcaaatgc agccccttgg atcactgaat aaatgacata
    52141 taatggcaca gcagcaaaca taaacatgaa ctccaatggt tctgttacac ctgttagaaa
    52201 gacagccaac gctgctgaaa aatacattga tttatatctt gctttcttat caggatcgac
    52261 attacgatac atcgcaaaag ccattcccat caagatacct gaagaaccaa tcatttgtcc
    52321 tactttgaaa cgagccggcg tccagttatg caacacaaac tcatattgcg acatatcccc
    52381 tgcaccttta agattcacta aatcagtggc ccaagccaac cataacggat cttgcccaaa
    52441 tacttgtgtc cccgcctgtg cgcctgataa aatctcatac gatccgccaa gctgtgtata
    52501 gttgatagga atcgtcagca tatgatgcaa tccaaatggc aatagtaaac gttctaatgt
    52561 tccaaataaa aacggtgcta gaatcggtgc gctttcttgt gattctgcaa tccataaacc
    52621 aaaattattg attccagcct gtacgtttgg ccacaccaca gcaagaatca atgccacgat
    52681 tgttgaccat aagataacaa caaacggaac aaagcgcttt ccattgaaaa atgacaaagc
    52741 atccggcaat tttctataat tatagtactt attataagcc attgccccga caaatccagc
    52801 gatgatccct acgaatactc ccatattcaa agctggtgct tccaatacac ttgtaaaaaa
    52861 tcctttaacc atgatcttcg taccgaacag agtgtgggta aatgcatctt cattcgcaag
    52921 catgtcactt gtgacaccaa agatagcccc agtgatccga ttgattaaaa taaatgagat
    52981 cccagctgca aatgctccgc ctgctctttc ttttgcccaa cttcctccta ttgctaaagc
    53041 aaataaaata tgaagattac caataattgc ccaaccaata ttttcaatta ctccgcccgt
    53101 tgtgacaagc ggcgtccaat caggattgat caagggaatt gatttcccta tactgatcat
    53161 caacccagct gccggcataa ctgctaccac aaccattaaa gcctttccaa atttttgcca
    53221 aaactcaaaa ctaaatatct ttttcattcc gtttagctcc tttacgcaat cggtttcgtt
    53281 aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt tattttttat ttttctgtct
    53341 ataccattct ataaaaccta taatacgtgt gttcccttct ataattttct cctaaaaatt
    53401 tattttttta agcttttttc tatgaaatat tttcttttgt ctattgactt gtgcaatcgg
    53461 ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga aacagatcaa
    53521 acgactattt gctattgatc catggaaaat cagcacgaca caattagata aaaaaaatct
    53581 tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc gcgggaactt
    53641 tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg tctggtatcc
    53701 cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg gaaaagtgat
    53761 caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga tcgacttggc
    53821 ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg tattatctcg
    53881 tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac gttttctgag
    53941 cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg aaggtagcgg
    54001 aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca gcaactatga
    54061 tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct atttgacgac
    54121 taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag caatgcgcca
    54181 tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag cattacatgg
    54241 ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag aaattatcgt
    54301 tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct cagaattaac
    54361 gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt gggcagaacg
    54421 atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag gtattcgatt
    54481 caatttgttc caactattct ctacttacta cggcgaagat gaacgattga atatcggccc
    54541 caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag aagcgtatgc
    54601 tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc tgaaatatcg
    54661 gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa aaggcgcgct
    54721 ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa tcacattcga
    54781 ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga attacaccgg
    54841 cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg cacgcttctg
    54901 ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg gtgtgaccgg
    54961 cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga tcgcagcttg
    55021 ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg ctatcactat
    55081 cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact tcccagttga
    55141 cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc tcatgcctgt
    55201 atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg acaaaatcct
    55261 tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct tcaatgatac
    55321 ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga ctgtccatga
    55381 gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa tggaaagcaa
    55441 agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca ataacgacac
    55501 agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg tccaaggatt
    55561 tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc caaaaacttg
    55621 gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct cagtcacaga
    55681 tgaacaagtt actttcaagt tattagaagg ttctcctcta gaaatttacg tctatgaaca
    55741 gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag caaatgttta
    55801 aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc cattatcgtg
    55861 cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc aacgaacagc
    55921 taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc ggaaaagaac
    55981 actcttttac aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat tattatttag
    56041 agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct ttactggatt
    56101 ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac gggccattcc
    56161 tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg gcagaagcaa
    56221 caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc ggactaaaag
    56281 cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc ctctcaagtg
    56341 gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc ccgcttgtcc
    56401 ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac catggataat
    56461 ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac attgaagaac
    56521 gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat cttcgcacca
    56581 gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga agatgttcta
    56641 acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat ccgggatgct
    56701 tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca cggtggcacg
    56761 aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc atcaataggt
    56821 gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac tgctacgatc
    56881 acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag ccttgttgaa
    56941 accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg aacaagagac
    57001 atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga tcaagaaaaa
    57061 ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag aaaatcccca
    57121 acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt tcttttgcat
    57181 catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg tcgccaaatg
    57241 actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt cgaagctgat
    57301 ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga accgcaagaa
    57361 atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc tggtcaagaa
    57421 cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg tgtttttatc
    57481 atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc gaccgtctct
    57541 cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt ccgaaaaatt
    57601 atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa acgcatttcc
    57661 agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg taacccattt
    57721 tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat gactacagca
    57781 atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat gcatttacaa
    57841 aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt cattgattat
    57901 ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca tgagaacgaa
    57961 atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga atttttgatc
    58021 cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag tttgtatttc
    58081 gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc gttatccagt
    58141 gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa agaaaacgaa
    58201 gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca gttggcgcaa
    58261 atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa ctcgatcttc
    58321 tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga attaggacgt
    58381 gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg cgtacgtttg
    58441 gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa cccatactaa
    58501 atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt atgtaaaaga
    58561 agatactact tctaacacat tgtttatctg taaaaaagag cgtgggacaa aagtaaagaa
    58621 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa ctccttcgga
    58681 aataagctga aattcacaaa aatttaaaaa gcaattttct tgagtttctt cttatttctt
    58741 ggagttaaac gcttctgtcc cagcctcttt tttccttcct tcaatttttt acacgtcgac
    58801 gattcaataa aaaaccgcct aatattgtaa agacaggtgc aagaatcgag aaaaaggcga
    58861 atggcaagta atgcaacgcg ctaactttca tcgttcccat aatgaatgtg ccgctgactc
    58921 cccacggtac gagagaattg acggtcgcac ctgaatccgc tagtgttcgt gtcaaatagg
    58981 tttttgataa gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga agaatgatgg
    59041 acagatactg ttctccaacg atcagattga ttccgataca gctcaaggca gtaaacccga
    59101 ctaaacgaga aggtcggtcc atcttttctt ttaattcttg aacgatggta tcaacgatca
    59161 gatatttgat cagtaaccca cctaatgcca atgcaagtaa aatcaaacta gcagaaccta
    59221 acatactcat gatcccgcct cgagaaagca gttcatctag ctttggatca ggtgtatttg
    59281 cgacataacc attcatcaag atgtctgctg cttttccgac cgtcaaactt ttctcatgga
    59341 taaaagaaag aacgagagca gctgtcgatc ccgctagtaa tgtagggata gccggaattt
    59401 ttttgattgc cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa
    59461 aagctcgctt tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg
    59521 ttatgtccta atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa
    59581 cgcataccag cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc
    59641 gtatctgata aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta
    59701 gtcattgcat cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc
    59761 gaaatcgttg taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct
    59821 gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg
    59881 gttccagata aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga
    59941 atgatccctg gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt
    60001 ttaaatctgc cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag
    60061 agctggaatc cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata
    60121 ctttctttta aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc
    60181 tgccatgaaa aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg
    60241 ctgaaagact tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa
    60301 tctttcttga agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct
    60361 actaataatg catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata
    60421 gagaagatca gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg
    60481 taaggacgtt cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa
    60541 atgtacacga tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt
    60601 acgtcatcca ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga
    60661 acgacatttc tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg
    60721 aatccttcac gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat
    60781 tgtaaaagca caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag
    60841 cctaggattt gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca
    60901 tctggtacga catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc
    60961 aaagcgataa atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa
    61021 acagaactca tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa
    61081 tcaatatgtt ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt
    61141 gagactagtc cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg
    61201 atatataccc ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag
    61261 ttcaagatcg tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta
    61321 ttgccaagtt ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc
    61381 attaccatac ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg
    61441 ctgcccagtg ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa
    61501 agctcaccgg aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa
    61561 aaaatcaatg tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt
    61621 gccattgtca gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc
    61681 gttgacactc tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa
    61741 taaacgaaac aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt
    61801 tcttcaaaga caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca
    61861 gatccaaaca tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg
    61921 cttattttgc ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa
    61981 gagctgcttt gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg
    62041 tcattacgct ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga
    62101 atcctagttg gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag
    62161 tatgtgaagt taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat
    62221 atacgtcatg gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata
    62281 catagtctac catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc
    62341 ctactttaaa tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg
    62401 cacccattaa tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa
    62461 cgctggctgc tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg
    62521 ggatatctgc gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc
    62581 ggatatcttt gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt
    62641 caatcgttac agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga
    62701 tataatcttt gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa
    62761 atggaatgaa gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag
    62821 catctacgtg taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga
    62881 ttttatcgat accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta
    62941 ggattggtgt tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca
    63001 tgcgatagtt gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt
    63061 cggcagcttt tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt
    63121 ctaaatgttt tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc
    63181 tgtctaacag attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc
    63241 cagcaactag ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg
    63301 cataccaaag tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat
    63361 ctttatagct cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg
    63421 caggagaaga ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta
    63481 gtaaagatgg catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc
    63541 aaggaacgga atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat
    63601 tcacagtgtg ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt
    63661 gaggcatgta gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt
    63721 tatagatttg gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt
    63781 tcgacaatga ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc
    63841 ctacattaca cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat
    63901 tttaatatgt atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg
    63961 gaatttacaa atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt
    64021 taggatgggt ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact
    64081 tactaaattc taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac
    64141 cacaaactta ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg
    64201 gtcaccgtta atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg
    64261 ttcaattttt gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac
    64321 gttcggcatt ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc
    64381 agagaacaat gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa
    64441 tcttgtaact tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac
    64501 ttttttcgct aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt
    64561 cactacatcg cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt
    64621 cttcggatca agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt
    64681 cagcatcaat gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat
    64741 taggtctaat ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac
    64801 atcgtatgtt ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc
    64861 tgtaaatgag attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt
    64921 gatgttgaag tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca
    64981 gtcgtagttg ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt
    65041 ggaaagtgcg tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt
    65101 gcggccgctt gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg
    65161 gcctgccaat tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga
    65221 atcaccagtt ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg
    65281 aagtctttct tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat
    65341 attcatttta ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt
    65401 aattaattat ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca
    65461 agggacgaaa tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg
    65521 aacatttaac gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt
    65581 aaagcatgta ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac
    65641 ttcgtttcga tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt
    65701 ctttgtcaac agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg
    65761 ctctaattcc gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt
    65821 tttctgcaat ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata
    65881 aggaatggga caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa
    65941 aaaattattt taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt
    66001 tttgtcatcc gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa
    66061 aaagcaaaag aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct
    66121 caaagctacg attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct
    66181 actgaaacac aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg
    66241 tctgatccta ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa
    66301 gccttccact ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc
    66361 aaccgttcga tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg
    66421 atccaaaaag acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa
    66481 aaaccattgg ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt
    66541 tttcctagcc gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa
    66601 gataaagaag aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa
    66661 catcctgatt tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga
    66721 gttttaggtt atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag
    66781 attaaagaag ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc
    66841 catacatggg gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat
    66901 gaattgtggc agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca
    66961 tttggtgcag acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac
    67021 ttgaagagtc aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga
    67081 caattaggta cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa
    67141 tctgatttag acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat
    67201 gacaaagaag cacgcgaatc tgcttgattt tacgtcattt atgaaccaaa aaagccgaac
    67261 tgacatttta tcgtcagttt cggctttttt atgctttatt tcttcttcgg tgcgattccc
    67321 ttgatcagga cttctgtcca ttctgctgta tatggaagca gttttgcgta tatctccggc
    67381 ttagaaattc cttctaccat tgcttgaaaa tagaggatca agaactcatc cgaatatttc
    67441 aaatccactt ttccttcttt ccgccctcga tggaaaagat caagcatgat actgaaactt
    67501 tcttgcgtat acgcatccat cgcgtcagaa aattcttttt tttctttttt tgtatagtat
    67561 gtcatcaaat cgatataaaa ttgtttgttc actcgattga gataacggat tttgttttca
    67621 ctcatggcaa tcagtgtttc ttcaaatgga agattttccg caatcaacgc tttcgcttct
    67681 tgccctaaat cattcataaa tgcaaaaaat acttcataaa tcagcccttc cttgctgcca
    67741 aaatatttaa aaatcgtcgc tttacttacc tgagcgttct cagctacctt atccattgtc
    67801 actccagcgg ttcctatgtc tgtgttcatc aagtcaaaag ctgcttgtaa aatctctttc
    67861 tttttctctt gtgctctctt ttcaaaaccg ttcatttgta gagcctcccg taattttaaa
    67921 cttttataat atatatagtt caaatttatt ctgaggttta ttataactat ctattgagaa
    67981 agtatcaagc tagcaattcc ttgtttatga attcattgac agaaatttat aaaaagaata
    68041 ttatgtacta aaataataaa aaaagtttta taaaaaggta taaaaccttt ttattaaact
    68101 atttaatctt gtttagtata tatttttaga aaaaggagtg gggtttttat ggcagaaatc
    68161 gtcaaagtca cacatttgaa aaagagcttt ggtcgtttcg aagctttaaa agatgtctct
    68221 ttcaccgtac attcaggtga agtcgttgga tttatcggac caaatggttc tggaaaatcc
    68281 acgacgattc ggacactttt agggattata aaaaaagacg gcggaacggc aacgattttt
    68341 gggcaagatg tatgggaaga tagtttatct atccatgaac gtctttctta tgttccaggc
    68401 gatatctctt tgtggggaaa tttgactgga ggagcgatca tcgatctctt catgaagctt
    68461 catggaaaag ggagtgctca aaaaaaagat gaactgattc aacgtttctc tcttgatcct
    68521 aagaaaaaag caaaaaacta ttcaaaagga aatcgccaaa aagtgggctt gattgctgct
    68581 ttggctgttg attcggatct ctatttattt gatgagccta cttctggttt agatcctttg
    68641 atggaaacga ttttccaaga agaagttgaa aaattgaaag aacagggcaa atccatccta
    68701 ctttcttctc atattctcag tgaagtcgag cgactagcgg ataaagttgt gatcatacgc
    68761 gaaggaacac tcgatgaaga aggaacactc gatgaattgc gtcatttgac tcgttcgacc
    68821 atcactatag aaacagaagc agagaatagt cacttagcac aattgaacgg tgtccatgac
    68881 tttgtgcaaa aaggtcacct cgctcaattt gctgctgata acgaagcttt cgatgtgatt
    68941 ttagctgaag cgtccaaatt aggtgtgaaa aaatttgaat ctattccacc gactcttgag
    69001 gatttattca tgagacatta tgaaggatga ggaggaagcg aaatgactga taaattttca
    69061 cgctggtcca cccttttttc tcaaaacctt agccgtgact ggaaaaagat cctgatatgg
    69121 ctagtcggtg tcactgcttt ttctggtggt tttgtccctg cgtttgaaga attgacttcc
    69181 ggacaaggat tagctggatt atacgaaacc ttgcaaaacc cggcaatgat tgcaatggtc
    69241 ggaccaactc cagcttcgaa tgctgctgag tatactttag gagcgatgta tgcaaatgaa
    69301 atgttgcttt tttgcagttt gtttgctgct gtgatctctg ttttacacgt ggtgagccaa
    69361 acgagaaaaa aagaagaact tggtctgatt gaacttattc gttcctttcg agtcggacgt
    69421 caagcaaact ctttagctgt catcgctgaa agtattctga tcaatttgct tttagcaata
    69481 tcgatcagtt gtacgatgat ttttttccaa gcggattcga tcactgcaga aggtgcttgg
    69541 ctttttgggc tatctgtagg tagtatgggg attttgggga gtgccgttgc actgtttgct
    69601 gcacaactga tgccaagttc agccagttcg aatggtctgt ctttagcgtt tttaggtgga
    69661 ctttaccttc tacgagcaat aacagatgta gctgattcct ccctttcgat gtggaatcct
    69721 ttaggctggt cttatttgac ctatccattt acaactaaca actggattcc tttgctttat
    69781 ttgtttttgt tcagtctaat cttgataatc agttcatttg ttttagagga acatcgagat
    69841 ttgaacaccg gctatatccc cgaacgtgaa ggaaaaccgt atgctaaacg ttctctttta
    69901 tccttgcgtg gtttattatt ctatctaaat aaagggatga caatcagttg gcttatcact
    69961 tttggtttga tgggggcggc atacggctct atctacggga atatgcaaca gtttttagaa
    70021 agtaacgact taatgaaaca gatgtttatc caatctggcg tctcaattga aaaaaacttt
    70081 acaagcacga tcatggtcgt cttgatttgt ctagctgcta ttttaccaat tgctagcatc
    70141 aatcgtttat ttacagaaga aacccacgga cgtatgaatc agctgttcgc aacaaaaatt
    70201 tctaggaacc aactttattg gaccaatatg ttactagcta ttctatttgc tatattggca
    70261 atcctgatta gttctggtgc actagggctt tctgctttgg cttcgatgaa ggatgcgaaa
    70321 gacatggatc tattgatgtt tcttaaagcc gggttcaatt tcctgcctgc cgttttagtg
    70381 tttatcggaa ttagtggctt tctattcgga tggcttcctc gaatcggtaa acttgcttat
    70441 atctacttag cttactcctt tgcactcaat tactttggcg gaattttgga tttaccagaa
    70501 tggttttcca aaacagctgt ccctagctgg gttccacgga tgccaaacga ttcttttgat
    70561 ctttccgtgt tccttacgtt atcatgtatc agccttgcac tgttcgtcct tggttctatt
    70621 ggttataggc gaagagactt actagaagga taataaaaaa gcatgtacaa tttcatgcta
    70681 gcctccaaat ctgggtcaaa aatccaacat ttggaggtca tttttttatt tacaagtctt
    70741 ctaactgctc tttatcaatg atccagactt ctcgattctt tctttttaca taaccattct
    70801 cctctaataa tttaaatttt ctagataaag tttcgggggt cgtaccgata aatgcagcaa
    70861 gttctttcat tttcattggc aggaaaaagt gatccttccc tgtcgcttta gctaagtcaa
    70921 gcagatagac agcgactcgt tcttctactt tttccatcat caaaaaagtt gtctgctgct
    70981 gtacttttgt taatttctca acgttgattg ctaaaagttt cagactaagg gcgggataat
    71041 gcagtaagag ttccgagaaa tctttttgtc tcaatatgca aatttccgtt ttttctagtg
    71101 cttcgccaaa taaagactca tttggaacgc ctagcaactg gctctctccc tcatatccgc
    71161 ccggctctat tactcgtaaa agctgttcct ttccgctagc agataattga tacactttca
    71221 tactgccgga ggccacgatc actaattgag gttctccatt cggcgcaaga atctgttctc
    71281 ctttttccac atagtgatgt ttgacaagtg cgtgtacttt ttcttgatct tgctgtacta
    71341 agtgattgaa caaaggaaca aatgaaacac ataaatgatc atgactcata atctctcctc
    71401 cttctaaaca gctatttcta ataaagaaac ataacttccc ttattccaac tgataatttt
    71461 cttttatcat agcataaacg tcaagcgaac cagataccag caaatagagt ggatttcttt
    71521 ttgtcccttg ctttattttc cttcttttga gaccttaagt aattgattcc tatcaatttt
    71581 ttaaatattt actttttgaa taatcaaaaa caaaccttca ctcgcaagcc gagagtaagg
    71641 tttgtttttc gcattgctta tttcgtgttg tctattgaaa tttttccgca cctagctttt
    71701 gatatagtgg tgctagttct tgtaaggttt cagcggttat ttgctcaacg tgttcgattt
    71761 cttgcggata gaagaatagt ttacccattt gaaaggttcg gtctctcatc gcttttttca
    71821 actcttcttg tttctttgta tcggacaaat agtcagacaa agctaagtgg tcgtcaagtt
    71881 catgttctgg ttgcggccat atgtaataat ccttgatatc gactgttttg ctccactctt
    71941 tcagtatcgc aagcaactgg ttatacgctg caatgctacc atatcgttct tcgcttttat
    72001 aatgttggta catcaaatag atttggtatt gtttttgatt caacaaaact gctagacagg
    72061 cattctcgtc ttgacgagat tcgcttcggt acgcgctcca aaaatgactg cgtaaattcc
    72121 agccattcgt ccagctttcg attttaggtt tgctcatgcc gtacatatca ggcaaaagtg
    72181 cagctgtctg gagctgaatc tctttccatt tttgccagtg ttctttatat tctttcttga
    72241 tttgttcaat cttatctgtt ggatggctct ctttgagctg tttgaatgcg tagattggtc
    72301 gatcaaacaa ctgataatct gattttccaa gttccattgt ttcgcctctt tccgttatct
    72361 tttgtattct acctgaattt tgcgatatgg acaaatagag gttgtgaaaa agctgttctg
    72421 catcaagtca taagaacagc caaacaaaaa actgcgatga gcatcaccgc agttttattg
    72481 aacttaaggt tgattgatca catctaaaag cgttgaaaat aaaatggcac tagaagcagc
    72541 tcctgggtcg atatgtccga ttgcacgctc tcctagatag gaagctcgtc cttttttagc
    72601 taccaagact ttggtgtgtt cttttgcctg gtcaatgatt tcttttgtta aattccctgc
    72661 cttcaatgcc tctgtgactg gcagccaaac atctaacatc gttttatcgt cagcttctgc
    72721 ctttcctcgg ctttgaatac ccgctgttcc ctgctcgatc acttcgccta atttttcttg
    72781 agaatcaata gaatcgacct cttttgtcgc tttcatcatg ttcatgaatg cagatccgta
    72841 taacggacca gaagccccac cgactttcga tatcatcgcc atagagaata ctttgaatgt
    72901 atcgctgatt gtttcaggat gatcggcttg aaaagcttct tcataagctg aaacccctct
    72961 tcccatgtta tttccatggt ctccatcgcc aatcggggta tctagatcac tgagatattg
    73021 tttgttctga ttgattgcct ctgcaaattg cgtgagccat tcttggattt cttgaacggt
    73081 tagtttcata ttatcaccct tttatcttga gattttttac catgaaatcg tctgtacatt
    73141 gctttctaaa gaatctttcc attgtgtctc cgtcaagtcg atcagtgtta gagaaagacc
    73201 ttgcatatct agtgaggtca taaaattacc gacttttcgg aaaactacat tgacgcctct
    73261 ttcttccaac agagctaaaa catcattcat gaacacgaac tgttccatca aaggcgtccc
    73321 gcccattccg ttgactagta cacctgcttc ttttggtaaa ttcgagtaat catctaaaat
    73381 cttagtaaca agttcctttg ccagtacttt agaaggctgc atcttttctc gacgatagcc
    73441 tggctcaccg tggataccaa caccatactc gatctcatct tctcctagtt cgaatcctgg
    73501 tttccctact tctggtacag ttgctgctcg aagcgctaga ccgatcgtct tagttgcctg
    73561 tacgaccttt tctcccagtt ctttcaactc agctagggaa gcaccgtttc ttgctgcatc
    73621 gcccacgatt ttgtgtacaa ggacagtacc tgcaactccg cgttttccag cagtatacgt
    73681 actattttca acggcgatat catcatctac gatcacactt tctacttcaa tatcgtccat
    73741 agcagctaat tcctttgcca tatcgaagtt caatgcatcc cctgtataat tcttaacgat
    73801 cagcaaaact ccttttccac tatcggcttc cttaatagcc gtctgaatct gatctggagt
    73861 tggagaagta aacacatcac caagcaccgc tgcacttaac atcccttccc ctacaaaccc
    73921 ggcatgggat ggttcatgtc cgcttcctcc accagaaacc aaaccaactt gttccgcttt
    73981 ctgattcttg gctacaacac gggaagattc tacacgatga acaagttctg gatagctttt
    74041 tactaatcct tccagcattt cttcgacaat attgcttgga tcattcatga tttttttcaa
    74101 tttattccca tcctttcttt ccggcatttt gtgcttctgt taagatttgc tgagtatcat
    74161 ctgttatacc ggcagtgatg gctgctgcaa aagctccttc cactaaaggc gcatccacca
    74221 gatgataacg ttgctgctgc tcttcttcca acatgtcttt tgccagttcc gagttcaata
    74281 ccgcgctacc gatatccgca aaaacaaaat aactagctgc atcgcttgat tcattgactg
    74341 cttctacgat tttcatcggg tctgatccga tctcgccatt tattccgccg agagaaaaaa
    74401 tttgcacctc ttcggatttt tgcatctgct caatcatttc tttaatacca tctgttactt
    74461 tttgactatg agaaataagc aaaatacttt ttttcacaag aatcttcctt tcttttacat
    74521 agttgctaac gattggtttc gctttcatta atgaacaatt caagtatata ataaatttct
    74581 aattttggga aaatatgtgt agtgcttctt gaagtttgtt atactttaat taaaagagag
    74641 gagtgatcga atgattacaa caacaacgaa cagtgtagag ggaaaagccg tagtagctta
    74701 taaagggatc gtttttggtg aggtcattac tgggatccat gcatttaaag acttggaggc
    74761 ggggttgcga aatatctttg gcggccgctc aaaaagctat gaaaacgaac taatgggtgc
    74821 aagagaagaa gcacttgccg aaatggccga acgtgcaaaa gcattaggag ctgatgcgat
    74881 cattggcgta aaaatggact atgaagtatt aggcagcgac aatggcatgc tgatggtcac
    74941 atgtagtgga acagctgtct ccacgagata agtaggggac atctgtttta ataactaaag
    75001 agagatggga catgtagtgg cactcaagaa ttagattttt cagtctaact tcgaggtact
    75061 ctaccttccc atcctctctt ttttatttct ttttgcggat gattccgtaa gctgctgcga
    75121 ctaacacacc cgcccctgct aaggaataac tgacagaagc tgttttttca tttgtttttg
    75181 gcaaagtctg acttgttgat ttggatggct ctgtcgtttg ctgaactgtt tctgtcgtgc
    75241 ttccagaggt cactgatttt tctcctgccg aatgcaaaat atcttttatt cgttggaaac
    75301 tagctgctaa gtcagctttt tttgcttcaa aatcattagc aactgtttgt gcttcgctga
    75361 tctttccgtg gattgcttga ctggccgctg tgatatccgc ttgagtttga cttagctttc
    75421 cagaagctgt ttgagcaacc tgatttaatt gctgattgaa ttcttttgct tgttggatct
    75481 tcccgtcaag atctgtttgg acagcttccg cttttgcttt cgcttcggca agctttttct
    75541 caagttgtgc acggtcattt cccactttat ttttaagatc agctaaatct tgatcgattg
    75601 ctgcttggtc ttctttcaat ttgttttttg cagcttctaa tgcttcttcg agttcttttc
    75661 ttttattttc caagtcttgc aaatgatcga tttggtcaga agaggtagaa ctatttattg
    75721 atgtatcttc tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca ttggaagtca
    75781 tttctgtttc cgaggcaccg ttttcagcac cagaagagct tatggttgtt gaaggttctg
    75841 tttgagcagg ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa atactggaaa
    75901 cagcaattgg ccagattaac ggcttcattc tttttttcat cacttttccc atccttacta
    75961 ctttttaact aatttcttat tataagcaaa aatgtttgga agaaccataa acacaacagg
    76021 ctgtttgtct aaaaaacggt gcatctttta tttttttgta cgttccgggc ttttccatgt
    76081 cccgcttcca taacctaaaa tcgttttgac tgctggaatg aaagtcgtca cgatcgttat
    76141 cgcgttcact atccagaaca aaagcatata tagtggtgcg aaaagaaggt atttcaattt
    76201 tcttcgtcga tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg cgaccatttc
    76261 aaagcagatg aacacaaaac tgaatgtaaa agtaatataa atctgttcgt agtttccttg
    76321 atatacataa tgcccgatta atccagcaaa aagaactacg gaaatacaaa agaatattga
    76381 ccagacaata ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat gtttaaaagg
    76441 atggagcatc acttttttga aattcgtcag ccagacttct gttccacctt ttgcccaacg
    76501 ttttctgtgt cgatatagca tcttcaacgt cacaggtact tccatgaaaa agataatctc
    76561 aggagcaaaa acagataacc agtcatttaa ctgatgatcc caagcaatac tgatgtcttc
    76621 tgtcgctcga tcttgtctga atccgcccac atcgatcaag gcttcttttc gatacatcgt
    76681 atttgctcca ctgtacgcat aaagaccacc gaaaacagct gactgtgttc gtttaatgat
    76741 gcccacgata cttgagaatt caaccgtttg agattttgca atcaatttgg tacgattttg
    76801 gacatccata tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa tgaaataatt
    76861 cacataccta atcagtgcat ctggctccgg taccgtgtct gcatcattgc ttaaaatcaa
    76921 tttccctttt gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt tatttttttc
    76981 gatccgaacc acacgaagat tagcgtattt tttcatcagt ctagctaata tttctggtgt
    77041 ttggtccgtt gaaccatcgt ctgtcactaa tacctcatag ttgtgataat tcaatttcgt
    77101 catcaaatac tcgattgtat attcgatcac aatttcttca ttatgggctg gcaccatgat
    77161 cgtgatgaac ggttcgacac tcaagggaac atctacccat tctttttgct tatgtttgta
    77221 taaaaacaca taacaccaga caccaataaa ccatacaaat ccgcctaaaa tcggataaga
    77281 aacgagaaaa aaatacataa tagacaaaca agtatctaag aataaattac ctgtatgggt
    77341 gattgagatc atttcagttc caccccattt tctttataca aatctgcaat gaatgtcgta
    77401 tctagatttt tctcttcgga gacagaataa aaacggaccg tttctctctc ttcttttgtt
    77461 ccaaaacgtt ccgtatacac ttcttcaagt aattcttgtc tttttttcaa tttttcatct
    77521 tcatattcaa aagatttatt tacttttttg tatagatttc gattgttcca cctagtcaat
    77581 acaaatgagg taatcaggaa actgaaaaaa atcaatacaa aaaagccgat taagaaatac
    77641 agcattttct tttctgataa aaaagcgtac aaggaaaaat ccttggctct ttctggtctc
    77701 gttaatggga aaaagaccca tataaaagga agaacaacga tcatccatcc taaaagagcc
    77761 actaacgttt gccaaatctt taaccagtaa ttaccttttg aaaaatactt atctttcacc
    77821 aaaattgatg caacttcttc tcttgttact tttttcatat cctcacctca cgtttttttg
    77881 ttacgacgta ggagtttacc atacatttca tttcttttca cataaagtgt tcctaaattt
    77941 ttcctaagaa aaagaacaaa acatttctca tcagcaaatg aaaaatattt tgttcttttt
    78001 atcagttgtt ctttttattt tttatctctt aattatgttg ctctttccaa tacgacaatg
    78061 cttttcattc ctctagatat cgccgtgtac atttgtctgc gtcttctttg ctcatctcca
    78121 tcgttttcaa atggatataa gattacatga tcaaattcca gtccttttgc tagactcaaa
    78181 ggaatgattt ggcagttttc taatccatat gaattttttt taatcgattc ttgtagcgtt
    78241 tgggtttcct cttctgtttg ggtgataact gccgcttttt ctccttttag agaaaccaaa
    78301 atctcagcta gtattttcaa caattcttca gaggatcgaa aagagcggta ttcgggttct
    78361 tttccttgcg gtcggatcga tacgatgtcg atcttctctt gatccacagc ataggttttg
    78421 aaaagctctg tgattgctcc gctagaacgg taactatttc taagatcata ccgatggatc
    78481 ggcagatggt agtcgtcgaa acaacgcata atatcagcaa aagtagtgga agaattaaag
    78541 attgcctgat tctcgtcacc tactaaagta aagtgagttt ttggaaataa ttcgatcaat
    78601 agacctaatt gtgccagggt ataatcttgt acttcatcaa tcaatagata gcgaaatgct
    78661 ggtacattta ccttttcaac aagtaaatga cgaatagcta atataatgac tgcttcatca
    78721 acagtacgtg tgggttgcca agcataagct gagccatatc gtttttcata aattctttca
    78781 aacaactgat gctcatccac ccatgccatt tcttcgattt gtctggttat ttttcgatat
    78841 tttttttgta aaagtttcct tgcataagct acaatacttt gttccgaatc atcagaaatc
    78901 aatttaccga ataattccaa ctgcctatct tctgtcaacg agagtacctg attctgcaca
    78961 gcaggacttt ttgcttgttt caaaagatga ttcttccatt cgctcatcag tgcctttttc
    79021 gttgcttgaa gcctatcgat caatttagga tgagcaggaa ctttctcata tattttttcg
    79081 atcgtttttt tagaaaggac tgtttttcca tttttagtaa gtccacggat aaaattcgga
    79141 tgatctaaaa acaaaggatc tgattgtttt agattatcga taaacttttt gctacgcaaa
    79201 cgttctgtct gctccgatac attttctccc gtgatacgtt tgaaataagc ctcttcacct
    79261 tctatttcct ctgctgaaag ctgtgaaacc agctgtatga tggttagatt cagtggattt
    79321 ctttcaccta atgatggtag gacattcgaa atatagtcga taaaccgatg attaggtgat
    79381 agaatcagca agtcatcgga tgtcatcgta tctcgatata aatagagcag atacgcaata
    79441 cgctgcatga tagcagaggt tttaccactt cctgctacgc catttaccaa gatattttga
    79501 cttttcacat cccgaatgat tgtgttttgt tccgattgga tcgtagcagt gatagccttc
    79561 atttcattgg tctcatcttg gcctaagact tctaataata cgtcatcttg aatagctaca
    79621 gacgtatcaa aaaacttgac caaacgatct ctttccaaaa gaaattgtcg tctttcttta
    79681 ataaccgtat cgattttatt ttgacgaacc gtataagaag atggaccaat catgttattg
    79741 taaaaaagtt cggcaattgg tgaacgccaa tcataaacaa gattttcatc tgcatcatct
    79801 gtgaaatttg ctgtaccgat ataaaaggat tcttttggct cattatccag aaagtctaca
    79861 acgacttttc caaagtaggg acttttcaat aaacgttcga tctttgctag ctgattttct
    79921 gcgtttttct gtttgatatt catctgatcg atttctctat ttttcatttc aatcatcgaa
    79981 aaagtatcca ggttatctaa gtaactgtca aaattcaatc gaagctcgcc agccatttct
    80041 ttcaacgact ggtttccttc tatttttgcc gtatccatcg attcccgcag ttcgtttttt
    80101 ttatcaatta gcttttgata cacgttcttc agatatgctt cttctaactg tttttgtgtg
    80161 ttgaccatac acattctcct ctttttttaa agtggtggaa ccgattagtt tacctctttt
    80221 aagagtcgaa tgcaagcttt ttaacgagta tcatttgtta ggatatatca gttcttcagg
    80281 tcggacagtt tgatataaag aaaatgcacc atcaaggttt tttacgtggt agccagactg
    80341 cttcaagatt ctttccgcta gatagctacg taatccgcta tgacagctga cgatatattc
    80401 ttgttggctg tctaattcat ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc
    80461 ttccttgaat ttgccctttt ccaattcttc tgctgttcgc acatctaata aaatttttcc
    80521 ttttgctagt tctgtcggca attcatgcca ttgtacggtt tcactaagcc cttctgcaat
    80581 gttcattgct gcatatccta gcatgtttac tggatctttg gctgagccaa atggcggtgc
    80641 atacgtcaat tctaattccg gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc
    80701 taaaatatcg atccgtttat ccacaccttt tgccccaaca ccttgtgcac cataaatctc
    80761 gccagtctct ggatggaaga tcaacttcag taaaatatct gttgcttctg gataatagct
    80821 tgcatgatct tttcctgata catgaatgac ttcaaaagac agttcgtttt ctttggctgt
    80881 tctttcactt aaaccggtgg atgcggcagt cattccaaac gttcgtataa ttgctgttcc
    80941 aataccgcct tgatttcttc gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg
    81001 acgattggct ggtgaagcaa gagaaataag tgcatctttt ccagtgattt gctgttttac
    81061 aacgatagca tcccccaccg cataaatatc aggaatattc gtttgataac gttcgtctac
    81121 taggataccc ccgcgtatgc ctaattcgat tccagcggtc actgctagct ggttatcagg
    81181 acgaacacca atggataaaa taactaaatc agcagggatg gtacgcccat catccagtcg
    81241 caatcgtttc ccgtggtctt caaatccagc gacagcatga gaagtaatca cttctactcc
    81301 ttgatgagac aattcttttt ctataaaagc tgccatttct tcatccagtg ttggaagaat
    81361 atgtggtgct tgttcgatca ccatgacttc taaacctctt ctttttaggt tttctgccat
    81421 ttccaatccg atgaatcctg caccaacgat cacggcacgc tttggctgtt tttctaatgc
    81481 atgcatcact ttatcgatat ctggtacatt tctaatagaa aaaacattgt cggcttctgt
    81541 tatccctgga atcgatggaa caactggttt tgcaccagga gataaaacca atgcatcgta
    81601 ctgttcaatg tgtttttgac cctcatgtac gacttctacc gttttgtttt cgggaaagat
    81661 ggctgtcact tcatgatttg gacgcacatc taaacaaaaa cgagcggata atgattctgg
    81721 cgtttggaca agaagatttt cccgttcact gatttctccc gaaagataat aaggcaagcc
    81781 gcaatttgca aaagaaacat acggtccttt ttcaaaaaca acgatttctg catcttccat
    81841 taatcggcga agccgtgtag cagcagacat tccacctgct acacctccga caataacgat
    81901 tttcatctta tttccctcct actgttcgat caaaccattg attcatgcca ccacgtacat
    81961 tcactacatc ataaccagat ttctttaatt ctttagctgc ttgtttactc ctcatccctg
    82021 attgacaaat cacatagacg gtcttttctt tgtctccatc atatctgttt atactttgta
    82081 atggaacatt tttagctcct tttatatgcc cgccccggta ttctgcaggt gttctaacat
    82141 caagtaacgc tggattttcc aaaagctttt cctttaattc tgggactgtt atcgatttta
    82201 ttgattgaaa tagcaaattc attttctgca cctcatttac atttttataa atacctcatg
    82261 gggtatatta aacaggagct ttattaatat gtcaaccaga atgactcttc atttttttca
    82321 ctacttttta tatttgcata aaataccgat aaggggtatt atctaattat aaaaatagag
    82381 gagaatgtgt tatggagatt gataataaaa atatcctgaa ccgtttgaaa cgtacagagg
    82441 gacaaattcg tggtatccaa aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca
    82501 ctcaattaag cgctgttcgt tctagtattg atcgcgtaat gggcatgatc gttgcagata
    82561 atctgaaaaa ttgctttgag aaacctgata gtaatccaga agaacaagcg gctaaactag
    82621 aacaagcgat caagatgatt attaaaaagt aaaggaatga tttcaatgaa agtcgttttc
    82681 catattgatg aattagaaaa atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa
    82741 gcttctccag aaacgacgat tgtcgtatct gtaaatggca tcgcgattac cggttatcta
    82801 gactctgcca atgcagaatt cttagatttg caaggagtcg tttttcacgc ttgcgccaat
    82861 gctatgcgtg ctaatcatat ttcagaaagt tctttacctg aacaagtaat cgttgtacca
    82921 gcaggtgtcc ttgatttagt tgaactgcaa tcccaaggtt atgcttatat caagccttag
    82981 tcagtagagg ttgcgacaga agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga
    83041 agctacttct ggaaaaccgt gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat
    83101 aagaacagcc aaacaaaaat agcttctcat atttttcgtt tggttgggct tattaccgca
    83161 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga aatcagcggt ttgacctaag
    83221 tattagagaa aaaaacggga aaatagctcc tcatattttg ccgttttttg agctaatacc
    83281 gaaggtcagc tgattgaaca ccgtttaagg aagagattgt gaaaaagtcg cttagtttcg
    83341 agtattaaga aaaattttgt aaaaatggat ttctacattt ttgcataaat ttggcttaat
    83401 accgagaaac tggcttttga ataccgttta aggaagaggt tgtaaaatca gcggtttgac
    83461 ctgagtatta gagaaacaaa cggaaaaata gctgctcata ttttgccgtt ttttgagcta
    83521 ataccgaagg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac
    83581 tccgaatcac aagaaataat ttcaaaaaca acttctcctg tttttggttc ggttggctta
    83641 ttatcgagaa gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac
    83701 accccttttt tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga
    83761 ttgtaaaaga ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc
    83821 tgggaaaagc agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata
    83881 ccacttcgct ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat
    83941 aaagccttct tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc
    84001 catagacaaa atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc
    84061 taagtctgtt acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc
    84121 tgagacaaca atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata
    84181 aatcggcagc aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt
    84241 acgccagtct gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt
    84301 attttagctt acacggggaa taaaaccaat gatttcactg tttttatttt tctttacaga
    84361 cactgatatt atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt
    84421 tcttcctgtc gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc
    84481 tctaggtcac gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa
    84541 cctgtcgggt acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg
    84601 attatttttt tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac
    84661 gcttcttttt ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctgacgttct
    84721 tttttattct ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc
    84781 cgataggctt tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc
    84841 attttatgag caatccacaa agcttggctt tctcctgttt cttccaaccc gcaactgata
    84901 tttaggacga agtgcttccc gatcgaaagc catagcagcc ggcacaaaat cttcatgatc
    84961 tccggcgaat ttcttgattt ctccataatg ggtcgtggca atgatcaatg ccccttgctc
    85021 atacatagat tccatgattg cgatcgctag tgcagcccct tcatttggct ctgttccact
    85081 gccaatctca tctaaaagta ccaacgtatt cctttttatc tttttcaaaa tagcagcgat
    85141 gttttgcata tgaccagaaa atgtactcaa cgcattttct aaattttggt ggtcaccgac
    85201 atcgacgaaa atctcatcaa agacagcaag ctctgtccct tcttttgccg gaacttgcat
    85261 accgaacatc gccatcaaag tcaataaacc gactgtcttc aacacgactg ttttgcctcc
    85321 ggcatttgct ccggtgataa tcaagcctcg atagtcttta ccgatttcaa catccaatgg
    85381 taccgcatgc tcaggtaaaa atggatgtct gccttgcttg ataatgatat gttccgattt
    85441 attgattcta ggtgtgcttc cattgatttc tcggctaaat tttgcccgag caaagatgat
    85501 atctaacacc gtgatcgttt cgatcaataa atcgatcact tcttcgtttt ctgcgatagc
    85561 accagtcaat gccgccagga cttgatattc ttcactgatt tcttcagctt ttaacaactg
    85621 atagtgttca ttcaatttag aaatcacagt tggttcaata aaaaccgtcg ttcttttatt
    85681 tgattcatcg ataatcgttc catctatttt atttttatag ctggctttga taggaatcgt
    85741 atagtattcg cctttttgaa caatcattgc ttcttggatc atttctttgt ttttcggatg
    85801 acgcaagaat ttcagtaatt ttgactggat ttctttttcg attagttgta attgtttgcg
    85861 tactttacgc aaattacgtg acgcgtcgtc actgacttta ttatttttta ttttctggtc
    85921 aatcaactct tctacattta tcaaatcagg aagatgtttg ctataagcaa acaacaaggg
    85981 tgtctggtat tgattcttat caaaaaactt tgtgatcatc cgactgcttc taagaaaatc
    86041 cgcatattcg atcaaatcgg ttggctgcag aatcaaccct tttttgactt gttctgtcag
    86101 tgcatcgatc ctcggcaacc ccataaatgg aacatgctga ctgctttcta agatcaagcg
    86161 tgcttctctt gtttcttctt gacgatccaa gacagtttgt aggtttgtcg agaccgtcat
    86221 atcgctgatt cgttttttgc tatagtttcc aattgctctt gcttggattt cctttttgat
    86281 ccgatcaaat tgtaattgac taatcatttc gttgtccata ttctctcctt ttcttgaaga
    86341 gatacctgtc ccaaaataaa taagggccta gagataaata aaaggataga acacagctta
    86401 aaaaagtcat gtcctatcct tattagaata caaagatatt gttttcctga caggtaccgt
    86461 ttcataagca caccaaataa actttgttta ttggctattc gacttactga ttagatacca
    86521 gctacactca caaaacatac cccattcttc taggtcttat ttattttcca cttaactata
    86581 gcaagacaca ttttggttgt caaactttag tatgttttat agatttacac cattaatgaa
    86641 tagacaccta ccgcgataat tgctccgatc attggacctg cgattggtac ccatgaatat
    86701 gcccaatctg aagttccttt attagctatt ggcaataccg catgtgctaa tcgaggacca
    86761 aagtcccgag cagggttgat tgcatatcct gttggccctc ctaatgaaag accgatggct
    86821 aagatcaatc cacctacagc gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa
    86881 gctagcaacc caagaactaa tacaaatgta ccaattaatt ctgtgatcac atttgctgga
    86941 taattccgta tagcaggacc tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt
    87001 gcattccaat gtggcaaata agaaagccaa acaagaatgg cccctgcaaa agctcctaaa
    87061 acttgcgcaa tgatgaaagg aacgacaaga ttccaactaa ttgcccctgt catcgccata
    87121 gctagagaga ctgcaggatt caaatgagct gggcccatga atccagaaat atagacagcc
    87181 attgttacag cgagtcccca accaaatgcg atgacaatcc agccagaagc ctgtgcttta
    87241 cttttgttta aattcacagc cgcacacaca ccatctccaa gcaaaactag gatcagtgtc
    87301 ccaataaact cccccatgat ttgagtcata tcactgttca tttatttctc cccctttagt
    87361 tcccgcagat ctgattctgc aatcaatgct tctaaacgtt tagtctgttg cgccttctcc
    87421 tcttctgtcc attcaaaata tccagcaatt gcatcgatca caggttgctt gatctcatca
    87481 agctgatctc gttcaaacaa cagatgattt gtacgacgaa tgagataatc gcctggggcc
    87541 aaaaccattt cttcttctaa accataacgt aaccgagctg actcagcgag actcaaacca
    87601 ggataaggtg ccatttcttt tgctaattca aagatacgtc gggcattagt cccgtaaaaa
    87661 tcagcgatat aagtagcatc ttcttcctct aaaccggctg ctactccttc ttttgctaat
    87721 tctgtcactg tttcttctaa tttagttgga tcgaagtttc cacctgaaat ctgatatttt
    87781 ttagagtcga tttctttcgt ctctattccg tattcttctt ttaataattg gcgtatcaat
    87841 cgcaaagctc cttctgccat cttacggtaa tcggtgattt tcccaccaga taaagtcagc
    87901 aaaccatctg gttctctttc taaagaacta cctctggaaa tcgtagaagg tgctttttca
    87961 tcacggctgt tttccaaatg gttcaacacg tcttctactt cagcacgaga aactttgttt
    88021 tccttatatt cacttactgt atcgacaact ttattgaagc tcttgtctga aatcgatcca
    88081 ttatctccac cattgtaatc agaaccagaa ttaccaatca acagtggacg aagtcctgcc
    88141 cagcttgctt cgatatcatt caatgtgata tttgcttctg gatagcgatg attgatcaca
    88201 tccaatagat aatctacatc ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa
    88261 tccgtatctg tcgtaccaaa gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc
    88321 ccatcttgct ttcctgtatc gaagtatgtg ggttgcggta caggcagttt tttcgcatct
    88381 acaactaaat ggatcccttt agttggacgc attttaggag agactggacg cgtaaaattc
    88441 aagtttcgta ctttatctac ccaaggacca ctcgtattga tcactaattt tgctttgatt
    88501 tcgatcactt catctgtcag caggtcacga gctttgacgc caacgatttg atcgccatca
    88561 tataaaaagc caaccgcttt cattttactt actagataag ccccatcttc ggcagccttt
    88621 ttgatattat caatcactaa acgggcatca ttgttgcgga aatctagata gacacccgcg
    88681 ccttttagcc cttctttttt caaaaacggt tctctttcca atacttcttc tggtgtcagc
    88741 gtataattct catatttggt tcctgtcaca tttgccagtt tgtcataaag gtccattgct
    88801 actttgaccg agaacatatt gaaggttgtt gctccttcgc cctcatagat tggcaaaagc
    88861 attggatctg gttttgggat gtgtggggca atactttgta cgactgcacg ttcaccaact
    88921 gtgtcagcta ctacttccac atcaaatgtc ttcagataac gaataccgcc atgaacaagt
    88981 ttggtcgagc gagaggaagt cccttctgca aaatcctgca tttcgatcaa tcctgttttg
    89041 attcctgatg ctgctgactg gactgccaca ccggcaccag tgattccgcc accgatgatc
    89101 aacagatcca attcttcttg ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa
    89161 aacatatgga tttcctcctt attcaaatgc ttgggtagct tttactgctt ttttccaacc
    89221 gttgtaaagt ttttctcggc gctcttcctc catttgaact tcaaatagct tgccagcttc
    89281 ataaaattcg cggatctcgt ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa
    89341 tgctgcccct agcgccgttg tttccaagtt ttctgctcgt tggatcggta cattcaagat
    89401 atctgtttgg aattgcatca gatattcatt gtttgctgca ccgccatcta ctttcaatac
    89461 aggaatcttg attccagtgt catcttgcat cgtatcgatg atatctctca cttgataagc
    89521 aatcgactgt aaagtagctt taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa
    89581 catagcccct cgtgctgcct gatcccaata aggagcacct agcccaacga atgcaggaac
    89641 aacataaacc tcatcttcgc ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt
    89701 ttgcaacatt tgaagtccat ctctcagcca ttgaatcgaa gaaccagcca caaaaatact
    89761 tccttctaaa gcatagtaaa cctttccatt tatgccgtaa ccaattgtcg tcaataaatt
    89821 atttttggat aattgcggtt tttcaccggt attcatgaca ataaatgaac ctgtaccata
    89881 agtattcttg accatgcctg gttcgaatgc catttgtccg aataatgcgg cttgctggtc
    89941 tcctgccatc cctgaaattg gtgcttcgct tccataaaaa tgataacctt gcgtataacc
    90001 ataaacttct gagttagaag tgacttttgg taacatcact cgaggaatat ttagtaaatc
    90061 taaaatctcc tgatcccaat ctagctgatg gatattgaac aacatcgtcc gactggcatt
    90121 cgagtaatca gtaacatgtg cttgtccatc ggtcagtttc catactaacc aagaatcgat
    90181 cgttccaaac atcaattcgc ctttttcagc tcgttcttgc gcgccttcta catgatcaag
    90241 aatccagcgt atcttcgttg ctgagaaata ggcatcgatc actaaccctg ttttttcgtg
    90301 gaaaaattct tgatagcctt cattttttaa gttatctgca attccagctg actgtctgga
    90361 ttgccaaaca actgcatgat agattggacg tcctgtatct ttttcccata ttactgttgt
    90421 ttcacgctgg ttcgttatgc caatcccagc aatctgattt ggtttgatcc ctgattcgat
    90481 gaacgctccg gcaataactg actgtacaga attccagatt tcatttgcat catgttccac
    90541 ccaactttct tgcgggaaat attgcgtgaa ctctttttga gaactgccga tgtgtcttcc
    90601 tttcttgtca aagataattg ctcgtgagct agttgtacct tggtcaatcg ccatgatata
    90661 cgttgattct gtcattgtga ttccctccat tatatgcaaa cgctttcttt aactactctt
    90721 atcatactac tgttttaaaa aaagaaactg tcaaaatttt attctttttt tctttttttg
    90781 acagtttttt attatgaagc tattttttaa tcaattcttt tcttttttca gcgtctctat
    90841 tcttcttttc aatcgaataa tgtcgtaagg tgacgcaaat tctgacagat aatattcaca
    90901 ctgttttttg tttctctggc aaaatgtgat caccaaatca tattctttac cttcttcata
    90961 acttccaatc tcaataccgc cgattcccct cagttctctg atcagatatt gttgaaaagc
    91021 aatacgataa atcggcagac tttctaaatc gattccaatc actactggtt ttgcaatata
    91081 aaaatcgatc atcaaaagaa cattggcata ctggatcatc aggtaactaa gctcactatc
    91141 ttcagctctt ttcctcggca attgctgcac cgctgtttcc tgtaatttcg ccagtaactg
    91201 tgttaaggaa tgcccgagca ttttctgctg ctgttccaaa agccgatcac gctcatagac
    91261 ttcgatcttt ccaacaaaga aaaaccattc attgttgatc tgggaaattt ggtagcctac
    91321 tgccttttct tctttgattt tcaaacgacg aggacgataa tgccataaca tcgtttcccg
    91381 aatgtaagta tctaaaacag cggttggtaa ctttttgctc cgtgtcaaat catactgata
    91441 aaatatttct tcgtctacta tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc
    91501 gtatacattc agttctgctg ctgttcgact aagatgcatc acgatgattg gatcgattgc
    91561 ttgataaagc cggtccgact gatagagtaa tttcttttct ttcaaagttg cgtacgtact
    91621 tttttcattc aatagtcttt ttttcgtaat cccgagccag caagcaatct gtgcttctgc
    91681 tgatgccgta aacgttgttt ctaatacacg gtccaatcct agaatgaacg gtcttttttc
    91741 tggtgtattt tgcaaaaaaa gcggtcgctg atcttccggg atatattgga atagttcata
    91801 ataaaaatat cgtatctgta gctcttctcc gtataattga ccgtttttga tttgtaatcc
    91861 aaattcctct aagagctggt tcaattcttt gatctttcga aaaacagaag actcactgat
    91921 attaaacgct gtcgctaatc gaacgatgga tacttgctga tgttctaata aatagacgag
    91981 catcttaaac ttcaagcttt cctgcaataa aaacaataga attttttcca aacttacatc
    92041 ttcgtccaac acaagcagcg cctgatactc atttcgctgt acttctactt ttttttccat
    92101 cattcggcca atcaattcaa tatcttccaa atattgatca aacgaggctt ttgacagttg
    92161 gacatgctct ctcaaaccat tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa
    92221 aatcaggatc tgtaactgct cttttctttc taataatgct tcaatcttca taggcagtat
    92281 tcgccacctt tgaccacacc atatctggca cacggtattc tggctggaaa aatagctcat
    92341 gttcttctaa ctgttcaaac gtccatcctt cttctactgc aattgtcagt tggtcaagaa
    92401 ggtggaacat ctcttttttg ctttggagct gtgctccaag aatctttttt tcagaatcaa
    92461 atagaaattt gagtttaact ttgctttgat ttattgctgt aagcttgtag tctacgtatc
    92521 ctgtttcaat tttttgggga taaaaaatgc tctcttcttc tgtcaaacct acgcttccta
    92581 agaaatagcc aaaccactga ttgccgactg gacgataggt tccctgatcc tttgtctgtg
    92641 agccactaat tgtcttgctg acatttcgtg ctgtacggac agcgttattt actagagaaa
    92701 catacatttt ttcatttgtt ggacgaaaag ttacttggat cgcatcgcca atcgcataaa
    92761 cgtctttttg agacgtttgc aagtagtcat ctacccagat cgttccatca tcattcaatg
    92821 ttaattgttc ttcccacatc tgattgtcgg gacgagaatg attagccagc aaaacaagat
    92881 catagttctc ctttcttttt tccgtctcta caatagcggt ttcattttcg gtcaaagaca
    92941 caacttgttc ttctaaaaaa agtgaaagcc cttgattttt catttccttt actaacggtt
    93001 ctgtcatttc aggatctaga taacgaaaaa gaattgactt tcggctttcg taaagatgga
    93061 tttgtttttt tcttgatgcc aacccttcag ctatttcgat tcctgcttgt cctgcaccaa
    93121 taattgcaat tttctttgca ttcatgatct tttgctgaag agccgttacg ttttgtatct
    93181 ttttaactgg gtgaataagc gttgattctg cagaaatatt tcgaaacttt tgatttgaac
    93241 cagtcgctag aatcaaacga tcaaaatcca atttttttcc tcctgctaaa acgacttttt
    93301 tttctttcaa tcctattact gtcttttctg tgtaaacatg gattcgatag cggtcagtca
    93361 gttcttcttt cgtgatccaa gaaagttgtt tttcactggt aacctttcct tttaatatca
    93421 aattgaaccc gctgggaatc catcctaact tcgattgctt ttcaatcaaa tatatttcac
    93481 ttgttggcat caattggcga agataaatag ccgcatgaat ccctgcaaac gacccgccga
    93541 caattacaat gttcatctgc attcctcttt cttataaggt gttctactgc taacttctca
    93601 atcagaaaac acagtattca taactcgctt tcaaccttat gatacatagc gatgcagcag
    93661 aatcaaccct ctccatcata ccgtgttcta ggtctgcttc tcaataatgt actagtggac
    93721 ctaattgaag agtatcattt ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa
    93781 aaaactcgcc ttttacgtac ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag
    93841 ttttagcgtg tgtaatgtaa tagttagaat agacacatca tttattttta attctttaat
    93901 gcttcaacaa ctgcttcgcc taattgatga gcagattgag gattttgccc tgtaagaagc
    93961 cgcccatcaa tttgaaccac ttctgaaaaa gctgctccag actgatagtt tgctcctttt
    94021 tctttcaaag catcttctgc taagaatggt acttcattcg ttgtaccatt tgcttcctct
    94081 tcttcattac tgaacccagt cactgtacgt ccagctaaaa tagattttcc agactcatct
    94141 gtaactggca acaatcctac aacaccgtga caaacagctg agaccagccc ttcatttcga
    94201 tagatttgtg aagcgacttt cccaagatct attgcttctg gaaaatccca cattgttccg
    94261 tggccaccag cataataaac cgctgcataa tcatttgcat ccacatcatc aggacgtaag
    94321 gtatttccca aggcccgatt tctgaaatct tcatcttcat aataggccca atcgatttca
    94381 tccatattca aaacactttg cggttctaaa ggtacatatc cgcctttagg actgacaaaa
    94441 tctgcatcga tcccattttt tactaataca tcataaaaat gagtcagttc acttaaccac
    94501 aaacctgtag gacgctcgat cttttcgtac ttttctacat ttgttaatac gatcaatact
    94561 ttcattttcc catcctcttt ctataaaaat tgcgttattg ataacgtgct ttcaagaaat
    94621 tatataatgg gcttggcaaa taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa
    94681 attcatcctc acggttttcc aataatttct ctgcccagtc aggatcgacg atcatttgct
    94741 gtccaattgc tgcaagttct gcatatttca atacctgctc tacatcttcg cgattgcgga
    94801 tcccaccgac accaactaaa gggatctttc cttgaatcgt gttatggacc acttcaagaa
    94861 tcgatttatc gttgtattta tcagaacgag ctattcgatc atacatattt aatgaaacat
    94921 gcagataatc tagttttgac tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac
    94981 ggatacctgg tgtttcatat tcttctggtg agaaacgata ccctacgatg aatggttttt
    95041 tagcatattg ttcaaccgct gagaaaaccg ttgttaataa ttcttcaata aaacgataac
    95101 gattttccaa gctaccgccc cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt
    95161 gttggatcaa atacgtattt gctccatgca actcgattcc atcaaatcct gattcaatcg
    95221 ctctacgtgt ggctttgcca aatgcttgga ttgtttcaac gatttcttct tcactcattg
    95281 ctcgtggtgt ttccgcatca ggacgttttg ctgcaactgc acttgcagat acaggctgtt
    95341 ctccagataa agttgctcga cttgtcattc gtccaccgtg gaaaatctga ataatcgctt
    95401 ttgcgccttc tccttggatt gctttcgcta gctcacttaa tcgaggaatc atttcatcat
    95461 gagcgatgct gagttcacct ggccagcctt ttccgatatc ttggacattt gcagcaccag
    95521 taataacagc accaagacct ttagaacgtt gtttataata agtgatctca tcttcggtga
    95581 tcactccatt gaaaaagctg agttgtgtcg tcataggcga cagcatcaat cggttcttca
    95641 attgaactcc tgaaggtaaa gtaattgatt gatcaaattg tgacattgtc attcctccca
    95701 taattcgctc tgattaacct atcgagaatc atctatcatt aaaccactcg attagttgta
    95761 catgcaacta atttgcttaa aaaattttat tttttttgta acttatctgt tgcatcattc
    95821 attgtctggg ctaattcttt acttgcttca tttcccaaaa ctgctttgta tgactgatga
    95881 agttcttccc agctaccatc gacttcctta gcaaattggc gtccttgttc tgtaagataa
    95941 actaatgaca agcgaccttc gctaactgtt ttgacgtatc ctttatattc taatttttca
    96001 acaaaacgag caatcgttga tggggcggaa tagagcattt gtgcaatatc tttttgtggt
    96061 attccatctt tttcttttaa tatcaataaa ataaaaccat aagttggtgc cagacctgtc
    96121 cttctaaacg cttcatcagc taatttattc aacatgcgat ctagcttttt cacgctaaag
    96181 aaaagacatt catctaacgc tttttcaatt ggcttttcca tttcatcccc tccattggcg
    96241 tacatatcat agtatgattt ttattttttg tcaaaaaaca cggaataatg tccgcctcct
    96301 ctgtctagat ttgaaaaaaa gaagtagcca tcttcaaaga atttcggctt gttttcgaca
    96361 aaactccttc tggaacaccg tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat
    96421 gaagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttggc ttaataccga
    96481 aaaactggct tttgaacacc gtttatatca gaggttgtga aaaagccgtc ctgcatcaag
    96541 taataagaac agccaaacaa aaatagttcc tcatattttg tcgttttttg agctaatgcc
    96601 gatggtcagc tgattgtaca ccgtttattg agaggtcgtg agcctgccgt tgaactccga
    96661 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttt atgactatgg tgacttgtga
    96721 tcgaggagtt ggcaggagaa tactgtttat tcgtaaagaa ggggctgtga caagactttt
    96781 gtcacagtcc cttatttcct tttattatag gatttcgcca tttgattgga tgacttgtct
    96841 gtaccaatca aatgattttt tctttaaccg tttctttgtg ccattccctt cgtcatcttg
    96901 atcaacatag atatatccat aacgttttga catttgtgaa gtagaagcgc tgacgatatc
    96961 aatgcagccc cacgacgtat aaccaaataa atctactcca tctttgatag cttccttcat
    97021 ctgctcaata tgctgcttca aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc
    97081 ttctactttg tctatagctc cgagtccgtt ttcagctatg aacagtggtt tttgatacct
    97141 atcatataat tgattcaaag caattctcaa acccttagga tcaatctgcc agccccattc
    97201 acttgatgga agatacggat ttttaattcc tccagttatt aagtttcctt ggaccccttc
    97261 ccaattttct ggatgagcac tgactgcact agacatgtag tagctaaaag aaacaaaatc
    97321 aacagggtag gctgctaaga tttcttcatc acctacttct atttttaagt tgacaccttt
    97381 ttcttcaaac attcgttgcg tatacgctgg ataatatcct cttgcttgaa catcactaaa
    97441 gaacaaagaa gttctttgct gctcaattgt tttttgaata tcatccggat cacaagaata
    97501 cggaacaaga agctgatagc tgatcataca accgacttgt gaacctttga taatttcatg
    97561 acaagctttt gtagctaaag cacttgctat aaactgatgg tgtgcacctt ggtatttcgc
    97621 ttgttccaaa tggggatggt tttcttcaat tactcctacg ctaacaaaag ggtgatgttt
    97681 tacacagttg atctcattga aagtcatcca atattttact ttatttttat agcgtttgaa
    97741 tactgtttca gcgaaacgaa cgtaaaactg aataagcttt cgattaggcc atccaccata
    97801 ttctgtaacc aaatataacg gcatttcata atgggaaaga gaaacgactg gctcaatccc
    97861 atattttaga cattcgtcaa acacttgatc ataaaaaaat aatcccgctt cattgggtgt
    97921 ttcttcatca ccatgaggaa aaattcttgt ccatgcgata gacatccgat agcatttaaa
    97981 acccatttca gcaaataaac ggatgtcttc tttaaagtga tgatagaaat catttccgtg
    98041 tcttttagga taataatact tgctgttggg atcctgcgcc gcttttactt gctgatgcgt
    98101 catcatatgc cattgatctg tccatttttc tttaggtaaa tcgctgtcga atgtatacac
    98161 atcagacaca gataatcctt tttcatctac atcccatgca ccttcacatt gattggccgc
    98221 tgtcgctccg ccccataaaa atccttctgg aaattttttc atatcgatcc ccctaaactt
    98281 gctagttttc ttgtttgatt atttttctta tgactagtaa tgatattttt gaatctacta
    98341 ctatttgtaa caataaccat tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa
    98401 tctacttcca tcaataaatc gcctttacgt actttttttc catcagatac tgcactttga
    98461 aagtatttgc catttaaatt cactgtatca ataccgatat ggatcagtat ctctgctcct
    98521 tcttcactga ctaccccaat tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt
    98581 acagaactgt agatttttcc gtccgttgga atgatcgcaa acccttctcc taaagatcca
    98641 ctagcaaatg ttggatcgtt tacttcagat aaatcaatta tttcacctgc tgcaggagta
    98701 cttaacacta tatcctttga ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa
    98761 gttagcatac aggaactgat catcgctaag aaataactag caactagcca aacaaatcct
    98821 tcaccaatat aaatcgggaa agacaaaata cttcttgtag caactgccaa agcaccggct
    98881 cctgcggcac cagctactcc accaaatata gctccacata tacaagcaat gtaaaatggt
    98941 cgtttatatt taagagtcac accataaaca gcaggttcag tgattccaaa taatgctgtg
    99001 ataaatgcag ataaagataa actttttact ttcttatttt tcgttttcaa aaatacccca
    99061 agtactgctc ctgcttgcgc agctacggca gtcatacatg ctggacctag cgtatcacga
    99121 ccgtagaaag aaagattgtt ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc
    99181 actaacactt gccaaaatcc gccgataaca gcaccagata aaacagggct aaagttataa
    99241 acagctaaat atccagctgc aatcagctga cttgcataat ctgcaattgg acctacaact
    99301 aataaaataa ttgggaccat cacgatgata caaatgaatg gtgtcaataa atttcgaata
    99361 ctttcgtgca agcttttgcg aatcttaggc tcaatataag aaagaaccca tacagctaaa
    99421 attgctggaa taaccgtact tgtatacctt gctagaatca cgggcatccc tagaaatcgc
    99481 aaggttatgc tatcactata tgcactgaca atcgtaggat aaacgagagc tgctgcaata
    99541 accatagcga tgaaacgatc tgtattgaac ttttttgcag cagtatatgc caaaaatata
    99601 ggcaagaaat agaaaaatgc atcagctgct gcattcaaaa tgatataagt tcctgattca
    99661 gtagttaata aattcaaact cgtacataag gcgagtactc ctttgatcaa accacttcct
    99721 gccaagacgc ctaaagtcgg ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa
    99781 atgccttttt tctcagttcc tctctttcca ctagatgcat catcttctag tttcatttgt
    99841 ttggataata cgtcatacac ttcatttact gcatttccta ctacgacttg aaacattcct
    99901 ccagcttcga ctacagtgat cacaccttct agttgctcta ccttctcctt atctgccttc
    99961 ttattatctt ttagggtaaa gcgtaacctt gttgcacaat ggtaaacatt gctgacattt
   100021 tcttcgcctc cgacaaatcg gacaatatct ttcgctaatt gtgaatagtc catttttact
   100081 tcccccttgt taattaatgc cgtagcaatc cttcttacaa gataagtata gttcttagaa
   100141 aacggtttct ccattcaatt agtacccctt ctaatagcga acgaaactgt acaatagtgt
   100201 gtatggttta cttagccgag caaatcactc actgcttgaa agaaaacaag tatatcgttt
   100261 ctcttatagt caaaggagac aaaaaaaaca cgcttgatct tcctttgctg atgtataagt
   100321 ggtatctcaa gctgatactc tgtgattttt gtttctactt ggcttttgat aagacagcac
   100381 aacaaagtct cggcatttaa cggaagcata actaggtcag aactaggaga gattagttgc
   100441 tgaatggaat ctatcttttt atggattttt atttgatttt cttctttgta gtaatgatca
   100501 agcagttttg ttttcttttt ttcttcaact atggtcagct tttcacgcaa aatctgataa
   100561 atctcccaag attcaaactc atctagaaca ctaggtagat aaatcaaatc atcccactca
   100621 aaaataagct cttgctcagt agtcaaaatt gcatcatatt ccgttatttt gtttgattga
   100681 tttttgaaaa gatacactgg ttctatccgg cattctttga tccacatgtt catactttgt
   100741 tgaatctgtt tttttagtgt attcaaaata ctaattggct ggttactaac aatcagtatc
   100801 tccccttgtt ttcgaaaagg attcaacgct tcggcaagat acaaaacaaa ataagcgctt
   100861 tcagctaaat tgggacgtaa ttgaaaatgt attgggaaga aacgccgaat taaataaatt
   100921 tctaaaggat aacgggagat gatcttttgt acaaaatgat tcatggtatt atgtccggca
   100981 tccatcctcg ttttcatttg tttgatatgg gttagcaaaa attctgtttc atggaacaat
   101041 aacgaaacag gaagatctaa aaaacgaata atcgtttgct caaatgctct tagattcttc
   101101 tctatttctt tgtttttttc atcattaatc atcaaataat ttaactctaa tagtcgattt
   101161 tcaattgctt gtttttcttg attggaaaaa gaatagggta gttctgatga taactttttt
   101221 gtgagggtct ctaccatcgg aataaataat gtatacctgt ttgttttttc taaaagttct
   101281 tctcttcgaa gaatggtcaa aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa
   101341 acaatataat tatatgatat aagaacctct tttaatgtct tttggacagt cggcatgata
   101401 ttggaaaaaa ttgtactgat ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt
   101461 ccaacaagag ataagaaaat tctttttgaa ttttcttcag caatgatttt tagtcctttt
   101521 gtcgacgaaa cttccagttc taatttagga ttgcgctcga tccaacgcaa aatcgttttt
   101581 atttgtaatg atacggctgt ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg
   101641 acataccctt tttgaaacaa taagtacgtc acaatagttg aagctcggaa atccatgctg
   101701 gaatagattt tatgtttcgt cacctcttgt atgatttgct gtttagtttc agattcaatt
   101761 gattctaaaa aataacctga acgtttattt gactggataa tgattttctt ctcgtagata
   101821 gcaagcatat ttttgatttc agtttggatc gttttcttgt tggtacctaa agcttcagct
   101881 aattcttcgc ttgttatcca atgattctta tttcttaata ctacaagaat ttggagttgt
   101941 cgtttattta agtccatttg cttacacctc ctctttgtta taaacataca ggaaaaacct
   102001 ctcaaatgac aatagtcact tgcagaggtt taaaaatcat ttgaagaaag atctatattc
   102061 aaggttttta ccattagact ggataatttg tttataatat tcaaaactat cttttttcaa
   102121 tctcctaaag cttccgctcc catcattttg tctatccaca taaataaagc catatcgctt
   102181 cctcatttcc cctgttcctg ccgatacgat atcgattggt ccccaccata agtaaccaat
   102241 gatttggcaa ccatctttta ttgcctcagc aatttcctga agatgtattt ttagatactc
   102301 acaacgtttt ggatcgtgga ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc
   102361 attttcaact atgaataatg gtaattcata acggtctgtc aattcattca tgacataacg
   102421 aaaccctaga ggatcaattg gccaactcca aggttcggga gatttttcct tcaaaaaggg
   102481 attatcgaaa ccatttgctc cgccagtatc tacgcgcatt tctgcttctt ttttatagac
   102541 agcgctacga taataactaa atgcaatata atctgccgtg ttagcttgca agcactgttc
   102601 gtctccatct aacattactg gtgcacagtc ttcttccttc catgtcctgt aaatataagc
   102661 tggataatgc cctcggcaca tgacatcgga gaaaaaccat gaattgcgtt taaattgcag
   102721 tgaaccaaat acattctctg gatcacagtc atatgcataa gtcgctaatg cagaaaagct
   102781 acacataata cccatcttag catttggatc gattttcttt aataattgag tagcacgggc
   102841 gttcgctaga aacatatgat gacaggcttg ataaatcact ttttttgtta gtgtagcaat
   102901 gggttcaagt ttatctctag cagggaatgt aaccaatcca gcagctgcat aaggcatacg
   102961 aaaagcatta ttgatctcat taaaggtcat ccaatatttc actttccctt ggtagcgttc
   103021 aaagacggtc tgcgcataaa attcaaaaaa gtcgattaat tttcgatttt gccacccccc
   103081 atactctcct accaatgcta agggggtttc ataatgacta agagtaatta ctggctcgat
   103141 atcatatcga tttaatgtat tgattagtct gtcataaaat acgagaccag cctcatttgg
   103201 cttctgttcg tctcctcttg gaaatattcg tgcccatgag atgcttgttc gaaaagcttt
   103261 taaacctaac tccttcagta aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc
   103321 ttcatgagaa agataatgga gtgattcgtc taaagcaatc tcccaaatct ttttttcttc
   103381 attccactga atactaggat gctggttcat gatcccgata agcgtatctg tcacattagg
   103441 taatttgccg tcttccaacc atgcaccttc acattgatta gcagctactg ctccgcccca
   103501 taaaaaattt tctggaaata tttcctgatc cataactatc tctcctcgct atcaacaatt
   103561 tcttacttga agtatagctc gattcatcaa aacaagatat tctaactatt tcggcagaag
   103621 agaagaaaaa aattgaatgc tactcttcat ttttaatatt tatatataac aaatacataa
   103681 gacgatcccc gaaagaaaac actcgttaac catctaagca aacgatttat tttacttcca
   103741 acacaaatgc ttcataaggt cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat
   103801 aatttgacac aataaccctt aaagcttcac gtggaatagt gaacgtctgc ttttcactgg
   103861 acatattaca aataacgagc cattgctggt cttcatatat gcgagaatag gcaaagacat
   103921 gctcaggcgt cttttccact aattcatagt ctccccagac gatgatgtca tgttttctac
   103981 gcaaatcgat caatttcttg tacatataaa aaacagaatc ttgatctgct aacgcttctt
   104041 ctgcattgat ttctttataa tttggattga ctcgaagcca tggaattcca gtagtaaacc
   104101 cagcatgttc acgggcgttc cattgcatag gcgtcctagc attgtctcct cctttcgcct
   104161 tgattgattc caagatttcc tcttttgtaa atccagaaga aatcctttcg ttatacatat
   104221 tgattgtctc aatatctttt gcttccgcga tatcatcgat tgggcaattc gtcatgccta
   104281 actcttctcc ttgatagata taaggggtgc ctttcatcat atggagcaag atagcaaaca
   104341 tcttggcact ttcttttcga tacacatcgt catttcccca acgagaaaca attcgcggaa
   104401 gatcgtgatt attccaaaat aaactattcc atccttcatc acctaaagat gtttgccatt
   104461 tggataatac ttttttcagt tcagagatac tcaagggttt caaatcccat ttgtctttgc
   104521 cttcttgctg atctaatcct atgtgttcga attggaagac catggataat tcatttcttt
   104581 taggattgga ataaagttta gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca
   104641 tcagattttt gtcaccaaaa gttgcttgat tcatttcctg aagatactca tgcagtttag
   104701 gtccattact tgtgatttct ttatccggtt ctttgccaat catatcgatc acgtccatac
   104761 gaaagccccc aataccttta tcgatccaga aattcatcat ttgataaatc tcttggcgaa
   104821 cctgtttatt tttccaattc aaatcaggtt gacgcttgct atacaagtgc agaaaatatt
   104881 gccccgtttt ttcatccaac tgccaagcag aaccactgaa tattgagcgc aatctattag
   104941 gctcttttcc attgaccgga tcacgccaaa tataatagtc ccgataagga ttttctttat
   105001 ttttacgggc ctcaatgaac cacggatgtt catcagaagt atgattcacc accagatcca
   105061 tgatgatacg gatatttctt ttctttgctt cttcaatcag ttcttccatt tcttccatcg
   105121 taccgaattc tgccatgatt gcttgataat tactgatatc atacccattg tcatcattcg
   105181 gagactcgta tactggagaa agccaaattg catcaatacc taaatacgcc agataatcca
   105241 acctggaaat aatcccccgc aaatctccaa taccatcttg atttgaatct tgaaaacttc
   105301 ttggatagat ttgataaaca actgaatggt gccaccattt ttgttccatg attttcctcc
   105361 tataaatgga tgtttctatt ttttgcttaa tcatttattt tagtctctct cactaaaaat
   105421 tgtaacgctt tcaaatagct attgcacctt gttatcggta acagaataaa acaaaaaaac
   105481 agctgaggaa attccctcaa ctgttttttg aaaggattga tttatgcagg gaagaatgct
   105541 gatttcacaa cttctttata aacggtgttc actcagctgt cccttggtat tagctcaaaa
   105601 aacggcaaaa tataagaaac tattttttcg tttttttctc taatactcag gccagaacgc
   105661 tgatttcaca acctctttat ttcacattca atatccacat gaatcccaca aatacaaaga
   105721 acagaccata catgattgga gatacttctt ttcctctttt agctgctaac atggtaattg
   105781 gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa ggcatgccga
   105841 tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt tgtcgcaaag
   105901 attgtgccat caatactcct accataatca atgctggtgc agttacttgt gaagtaacaa
   105961 ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta gtaatagctg
   106021 tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat gcaccaacag
   106081 gagaagtacc aaataaggaa cccgcaagca tagctgttga atcagaagct aaagctttcc
   106141 ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat ccaactaaag
   106201 ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc cacatttgaa
   106261 gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc aaacttggtg
   106321 ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct aaaatcgttg
   106381 tagctgccat cccgatgaag atgccacctg gtacacgccg aacaagcaag atagcagtta
   106441 cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct aatcctacta
   106501 atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa gcgataaata
   106561 gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca atgatcattt
   106621 cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat acgccggcta
   106681 atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa aaagcattga
   106741 tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt aaaacacagc
   106801 cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt ccagaagcac
   106861 ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt gtaaatcctg
   106921 ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag gaaagtattt
   106981 tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg ttaaaatttc
   107041 gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa gtaaacaaaa
   107101 aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt atctcaaagt
   107161 tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat
   107221 tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttcttctttg
   107281 atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtatcgacc
   107341 atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc
   107401 tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact
   107461 gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc
   107521 ttcagggtca gaaaaattga aagtcaagat gccattgtta tctgtgattt ctcctggttc
   107581 ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag aaggcggtgg
   107641 tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc ctaattctaa
   107701 ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga
   107761 aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata
   107821 aagcatcaca cgtgtttttt gtatcatcat ttgacccttc tttctatttg attttctttc
   107881 ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat
   107941 tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa
   108001 aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg
   108061 attcatcttt ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt
   108121 ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa
   108181 tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt tctttgggct tcacgccagt
   108241 tgagacagcg acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg
   108301 gagcatcgga atctgcacct aaaggcagga atgattcgcc accttgggct tttcctaaat
   108361 gcagatttcc tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg
   108421 aaatactatt atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg
   108481 cacgtttcag taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa
   108541 atgcctgacg ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat
   108601 ttcttgcttc ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg
   108661 ttacgtggtt atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt
   108721 cccagaaatt ggtatccact attacttttt tcatctgttc acacctcttg attagaataa
   108781 ttgcttgatg aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct
   108841 gtatttgata aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac
   108901 accgagccaa atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa
   108961 aaatgtaccg ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa
   109021 aaaatgatca gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc
   109081 aggctcttga tcgatactca ctaggaaaaa atcggtttct ttatctttta gctcttctgt
   109141 aagtttttct agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtgaattatc
   109201 ttttccaaat gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga
   109261 aggcgtcatt tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt
   109321 attgcctttt ttgacaaaat tccaatcttc gctaacattc ttgcatactt tatttaaaag
   109381 atattccaat tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta
   109441 gttgttttct cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt
   109501 cttgattttt ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt
   109561 cttgattgct cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg
   109621 gatgattcca ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc
   109681 gacttctttg aattcgatat tgctaataga atctagcgct cgggcaatca ttccgatgtc
   109741 tcgaaggatt tcagccattt catcccacct ttattttgtt tgtaagacta gttcacctct
   109801 ttttgttgca aatgcaacta ttttcaataa aaaaatcaag ttctttttta ataactagta
   109861 aagtatattc ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt
   109921 tctaaaataa gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct
   109981 atactaaaaa atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg
   110041 tggatcgcac aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt
   110101 ttattcgtat caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca
   110161 tagacttctt tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga
   110221 agtgaccatt ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata
   110281 taggcacgaa catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt
   110341 atttcttcga aagtttcttt tcctatttca ctgattagtt ccaatgaaat cgtccggatc
   110401 tcctcaaaac gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt
   110461 gcgagtagtc ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca
   110521 aatgtatgac tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat
   110581 gaaatctgca ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct
   110641 ctggggctgt gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccaccaaa
   110701 cgaaaaatat gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt
   110761 tttcacaacc tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat
   110821 attcatcaaa catgagaagc tgtttttttg aaattgctcc ttgtgactcg gagctgaacg
   110881 acaggctcac gacctctcgg taaacagcgt tcaaaagcca atttttcgat attaagccat
   110941 ttctttgtta cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat
   111001 ccacttgagt atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat
   111061 attttcggtc ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg
   111121 ttcgatcttc caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat
   111181 ctgattcacg atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc
   111241 gaatcgtgat cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct
   111301 tatcactaaa aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa
   111361 gtgctttgta atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat
   111421 tgttaaaccc gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca
   111481 tatctggaaa tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac
   111541 cagccagaag aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc
   111601 aagttcataa acagtccaat cttatgcttg acaatttctt ttactgcttc atttgctgga
   111661 atcacattat ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact
   111721 ttcgtccagt tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc
   111781 aattcaggat aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca
   111841 actactgcat cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt
   111901 ccatgattcg taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc
   111961 actgcgtctt ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct
   112021 gtaccgccga ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct
   112081 actactgctt ttgtcttcag gatattttct ttgaaaggca gatgggaccc atcatacatg
   112141 acagaagtat atccgctgtc gattgcttct ttgatatcat caaagtctcg ggcgtggtct
   112201 aagtgcaaga ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac
   112261 acatgcatgc cgatatattt agcggtatcg acacttgttt gaataatgat cggcgaaccc
   112321 atttcttttg ctgcgcctag catagcagga agcatttcta ggttatgcgt attaaaagct
   112381 ccaactgtaa aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc
   112441 ttttattgcc accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg
   112501 tcgcatctca tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc
   112561 agcatctgca cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt
   112621 ctgtttattt tccaaactct tttctaaata ttggatggct tgatcgttca catacaattc
   112681 agaaaattta agtccaactt gttcataaag tttcgctaat ttatgcttat cttgggtagt
   112741 ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga
   112801 tagggctttt tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa
   112861 tccaaacatc attttcccta ctttcaatta aaatgctttc aaaactgggc ttaagatcca
   112921 ggcatataag agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgatgaatcc
   112981 catcgaatta aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc
   113041 aataccgcca atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt
   113101 ccctccggca aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg
   113161 caaagtaaac atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacagc
   113221 atttgggcta taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa
   113281 tttcatagct atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc
   113341 tagcaataca tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata
   113401 gttttgatct cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat
   113461 atatagcggg accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg
   113521 tgtcggcaac ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt
   113581 agcaacaccg gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt
   113641 tgatccagtg attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca
   113701 agctccgata aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc
   113761 aaagactgtc gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt
   113821 ccattttgtg aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc
   113881 aatatctcta cctaaaccca aatcattcat tgcttgacca ttgatagatt caatagaagg
   113941 gatgatccct tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc
   114001 aacgatgatg ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct ttaacgtgcc
   114061 agagatgact tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc
   114121 aatggtgatc gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgcccatgtt
   114181 tttaccctct tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt
   114241 ttttcttcaa tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga
   114301 tcaactgcga acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt
   114361 ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg
   114421 ttcaaaacat cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcaccgcat
   114481 acgaataaaa tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca
   114541 actactgaaa aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc
   114601 aacttctctt cgttgttgat caattcaatt agttctttca tgatgttcag atgcgagtag
   114661 gagtctactg ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa
   114721 tcgattggtt gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt
   114781 cgggcatggg ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact
   114841 gcacgaatca tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta
   114901 gcagctagtt caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg
   114961 tccgtcaaaa tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt
   115021 gtttcgaata atttttctaa cttgccatag atgattcgat ctactcggcc accactttct
   115081 ttcatgacat ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac
   115141 cgctgtagtt cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt
   115201 aaaatcggat cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa
   115261 accagattga catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta
   115321 atctcgatct gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc
   115381 ccatggttac agacaacgac tgatttataa acataattca agtctcgctt gattacactg
   115441 gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca
   115501 attacttctt cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt
   115561 gttcggatgt tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc
   115621 agtgcagcca tgtgcttata gagattttcg aataaagctt cttcctttag atggaaaggt
   115681 acgtgtgtct cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc
   115741 cactcgattg aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa
   115801 tttctttcta cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat
   115861 gcagaaaagc tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca
   115921 tgtttcctga ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata
   115981 tcatcttctt gtttcaacaa cttattggaa tagatctcct gtattttttt gatttccacc
   116041 aaaggaaaat aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata
   116101 aagaagtcgg tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat
   116161 cgttctgaac ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa
   116221 ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga
   116281 aattcttctt cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact
   116341 agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt
   116401 tctaatcgag cttgcttttc cttttccagc ttcaacaaaa aacctttgtt gcgaattgta
   116461 cggatctccg gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacgg
   116521 atcgtccgct gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg
   116581 gctgtcatga ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc
   116641 tgatttcaag tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag
   116701 tggaaagaaa gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac
   116761 gaaaaagaca tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt
   116821 gaaaaagctg tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt
   116881 ttcgtttggt tgttcttatt atcgcagatg caagctttta aacaccattt agtggagagg
   116941 ttgtgacaga gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat
   117001 attttacaaa atcgtgcctt attgccgata ggctatctct ggcacaccgt ttattcagaa
   117061 aaaggagctg cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca
   117121 gcataatcgg ctgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat
   117181 accatatctg agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt
   117241 ttcgatcgac tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt
   117301 ggatctgtcg ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac
   117361 cgtctgcttt gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac
   117421 agcttctggc tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct
   117481 tttggatttc cagaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata
   117541 tctccgcttt cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc
   117601 ctcctactga caaaacagct aatgcaagaa atcccgtttg ctcataacta gaaggacctg
   117661 tacctggacc ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc
   117721 ccccatatga acgaccacca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc
   117781 caccatgacc acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca
   117841 ctaacaaaat gattgctaaa taattgattt ttttcattgt cttcctcgtt atcttttatt
   117901 acaaaaaact atttttatga tagccttgct aaaaggaaga gtcaataacc agatgcataa
   117961 actgtttttt tagcaatttt gtcttattta gtcattgagt aacatcggac aaatgtatgt
   118021 gtcggcagtc cataattcct ctatcttcag catgaacaag cttgcagaca agagatctgc
   118081 gcttcctcca ggagagattt ggttttcctg acaaaagtgc aacagtgctg taaatggacg
   118141 atttggtaaa gcactttgac tagcagcatg agcaagtttt tgcatttcca ctaacgtttt
   118201 ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa ctaacaacat
   118261 atgcatttgt ttctgtacat atgaatcatt atctagctcc ttagctgctt gaattgcaag
   118321 cctgatatgc ggatacccta atatcgcttc ttcatatgca ccatttacgc catatttttg
   118381 cttcacccta tgaccatgag tggttttttt atgacgatat tttgtatcag gaaaagcagc
   118441 aagcttgctg acatcatcaa gtattttcga taaagaaaaa ggctgttcca tactgatttt
   118501 ttgtgcaatg acactgatca atagccccat tgcccaaatc gctcctttat gggtattgat
   118561 gccatttgta gcaatcagca ttgccttttc cgcttttcgc ccaatctcag caattctttc
   118621 tcttaactcc tgatcaatag catgatgcca tgctatttga gccatttctt caaaaaaagg
   118681 agttagcgtc ttagcagact taagaaataa agcatagtcc atatcggaat gtgcacccgc
   118741 atccagtgca tccactaatc caggttttgg acttaatgtt acttctgcga tcaatgcttc
   118801 ctctaccaac gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat ttatcatgac
   118861 actcactcct tcttcctact attacttgct gaaacctttt ttgatagcta cccaaatgat
   118921 atgaagtaac cagcttaaag caaatgcaca acttgccgtc attagataag ctacaagaat
   118981 catgactcct gaaggtaact gcagccaact atctttaaac aataataaca gtacttggaa
   119041 aacaaataca ttggctaaat acgtacgata agcataaaca gacaaccatt tgaacaatgg
   119101 caattttggc gaatcgttta caatcatttt ggaagcgcct atgaagacag caaagatgat
   119161 gactaaactg tataaacttt ggatcgtatt caagtaaggt gcattcgcaa agctcaagtc
   119221 tcctggatgt gcgagcaatg aacggacaga aaagaacgcg ataactaagg caacaggtaa
   119281 gaaaagataa cgtactctgt ccaaatattg atagactttt tcgtgataaa caaatgcggc
   119341 taccccataa atcatgaaac tagcaacaaa ccgatctaat aagtaccagc tgtcatgata
   119401 aggcccagtc aatacttgtg tttgatagaa cagataccat gcgacatata aaatcgttga
   119461 accaatcaat actggccata catatttttt attagcaaat actttgtaac cgagccaaac
   119521 aaaaaacggc atgaacaatt ggatctgcag catcataacg gtataccata aatgaggcgc
   119581 tccatcacct gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg gagtttgttg
   119641 ttggactgct ggaaatagcc aaagataagc cgtcgtccat agaagatacg gaacgactag
   119701 ttcaaaaaac ttttctttca aaaattccca ataagacaaa tgctgacttg tttttaccat
   119761 attataaaca attgcaaaaa taaacattgg tgctgtgtat ttaacgatga cgtaaaaggt
   119821 accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc tcaatatcgt
   119881 ttgcatcata acagccgtta cagctgatac tttctggaag tcacttacgt tgattttgat
   119941 ttttttgttt tcaaacatct ctttctctct ctttttcttt attaccaaag ttctttggtt
   120001 aatttaggtc cttcgactgt tttcaataga atttttttct ctggctctct caaatactct
   120061 tttagtgaaa aaccgccttg ccgagtcact acttgggtat cgaattgctc aaaaaaagca
   120121 ggatgcgatt gaattacctg aattggcaat tcaatcggtg aatttgcgta cagcagcaaa
   120181 tcaaaatcac tggtttcttt tactgccgga atcccagtcg ataattcaaa tcctaagctg
   120241 ccgccgattc cccatttaca tcctgataat atgtttctag cagcatgaaa ttgctgataa
   120301 acaggataat ccatgatttt ctcttgtcta tacgattctt gttcgatgat gtcccacgga
   120361 tgaatgactt gtttgacggc atgttttgag atttctgttg accatctctg attgcgggca
   120421 ggtccacgta aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg acgaactgtt
   120481 ccgaacatct tttcagttag aacgacccat tcaggcaagg tgaacccttc tatttcatgt
   120541 ttatcaaaaa ggacaatatc atgtgcttga atcatttttt atgcatccca ttcttcgtcc
   120601 attttggcac ggactttatt tgtttcgaca cgtccgaatg ttttggctgt ttcgtttgtg
   120661 tatcgatttg tcagcatcgt gtcatttgta ttgcggacat cttgaatggc ttcattgatt
   120721 gcttcgatga ctttgctgac attttgttct gaagcagctt ggtcggtgat atcttctagg
   120781 aaatgataga tcgctcctag ttttgtataa ttattgatat cgtaagcgat agctggaact
   120841 ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc agcactttct
   120901 ttagacatcg cttggacggt aatttcttta ttgtttaaag caatcaagcg gcttgattgt
   120961 aatccatgtg ctaaaaatgc acctgaaata gcatttcctg caatcaatcc aataactgga
   121021 tgtccagctt gacggagttt tgcataagca gcagcactat tagccaaagc cacatggata
   121081 ccgatcaatt cttcgttata gccgtatgct tggcttggta catcgatcac aacgatgatt
   121141 ggccgtttca cttttttgtc tttatcttct tcgtaaacgt gatccaaaat ttgtcccatg
   121201 cggaaacctt caactaaacc aacttcaccg tttcgtacac ggtacatcgg gtttttagga
   121261 tcaggtacga ttgatgtcac gatggcttct tctatgaatt tacttgattt accataatac
   121321 acagtactaa tagaagaagt tggatttttc atgccagtta gtgccgcaaa ccaatcgtaa
   121381 ccaacacttg ctgcagctgg ttcatttcct tctgctactt ctggtaagtc aattgatttt
   121441 gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt tgaaagatct
   121501 aaagaatcaa gtaatgataa ataaaatgct gagctttcac tgcgatgcgt gtcttttcgt
   121561 tcgtccattg ctgcgatgac agcagctttg attgcttcta cactatcagt cactaattca
   121621 tcaatgattt ttgattctac ccgttgacga gatcctaatg tattccaaat caagtctttg
   121681 tcactagaat caaattctcg aacgcctgct tcttgttcaa taacttctgg tccattcaaa
   121741 ccgactctcg ctttttttgt tgcgattaaa taactcatta atgctgaagt aatcgacata
   121801 ccgccaaatg atcctacacg tcctggaatc aaaccgatga ctggtacata ttgttttaga
   121861 gcaatgatta catttccgat ttctgaaata gataacagtc cgtagtttgc ttcttgcaaa
   121921 cgaacaccac ctgtatctag tacaatgact ggaaagatct catttccttt tttattttct
   121981 tctaacacat gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc gataccgcca
   122041 ccttggaatt ttccttccat agcgattact actgcttgtt tgccattgat cgtgccacgg
   122101 ctgacaacga tcccgtcatc actttcaggg acgattcctt gtgctggtaa acttggcgcg
   122161 atgatttgat cgaatggatc aatcaattca cgtccattgt caagtagagc gttcactcgt
   122221 tctctggcgt ttaattctac aaaactattt ttcatctgaa agttcctcca ttgcttgcgt
   122281 caatcgtagg tacacaactc ctggtgtagc accgaagtca ttgatcacga tatctgctga
   122341 aattggataa cgatcaaaaa atctcgttaa tacattgttc catacttcgc cgaagccatc
   122401 gcttcctgta caaattttta cagtagattc tgttttttct gttggaaaca tcaagatttc
   122461 caaatctcct gaaccgacca ctcctacatg gattttctta ttgatcggct gtttcgttgg
   122521 atatttataa gctaattgtt ccatggatct ctcccctttt tgtaaaacag agcatacacg
   122581 ttctgcttat tcgtctttct tcttccatct gggtaaatgg cgacaaaagg tttttctcgc
   122641 caattttatg accaagttct gaatttcatt ggtggttcat acaggccatc tgaccattcg
   122701 atcaaatcat cgatggtttg tgcagctaat aatgaacgtt tcgcttcact acgtttgatg
   122761 cctagatctt ctggtaattt cacgatgccg gctgcacgca agctatctag tgattctttc
   122821 gtgctagtca ttccaagagg tgtaacaccg ccaattgctg caattgctgc ttgtcgttct
   122881 tcttttgttt ttgctttgta caaataagca attccttctt ctgtcacgat atgtgtagta
   122941 tcttcgctat aaatcatcac tggtgcattg gctaagcctg cttgtttttg aacagctagt
   123001 gcatctaatt cttcgacaaa gactggtttt ttatttgaac catatgtttc aaccatttga
   123061 acaactaatt tttggccttt ccctaatgga tcatcattgt cgcgaagtga ttgccatgct
   123121 ggtgtagaat gacgacgacc gccaggatta tgtcccatgt taggcgcacc gccaaaacca
   123181 gataaacgtc cttttgttac ggtagaagag tttccttcgt aatctaattg caaggttgaa
   123241 ccgataaaca tatcgatggc atattgtcct gcagcttgtg ataatgttcg gttactgcgc
   123301 atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag cagcaatata tttttccata
   123361 ccaacttcgc caccaaaact atggatgctt tcaacccaac ctgattcgat ggctggaatc
   123421 atcgttggat gtgggttcaa tacccagttc ttcgcgattt tcccttttaa ccctagtgat
   123481 tcaccataag ttggcagtaa caattcgata gctgctgtat tgaatccaat accgtgattc
   123541 aaagattgta cgccgtgttc tgcgtagatt cctttgattg ccatcattgc cattagaatt
   123601 tgcaattctg tgatgttttg aggatctcgt gtgaataaag cttctaattg ataaggttta
   123661 tctgctttca cgacaacgtc tacccagtca ccagggatat ctacacgtgg taaagaatcg
   123721 acaacttcgt tgacttgaac gatcacgata ccatctttga aagctgcagc ttcgatgatt
   123781 gttggtgttt cttccgtatt gaaacctgtg tacaagttgc cgtctttatc tgctttatct
   123841 gcagctacta aaacaacatt tggaatcaaa tcgatgaata agcgtccata aagttccaaa
   123901 taagtatgga tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc gacacgcaag
   123961 ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga ttcctttttc aaaaatattc
   124021 aaatgttctg gtcgtgagat actagacatg atcaaatgca gatcattcac tttttctgca
   124081 tctacttttt caagactttc tgaaagaaaa cttgcttgtt tttgattgtc gccttctaat
   124141 acaactttgt cgccgggagc aatcagtttt tccattactt ctacgatttt ttcagtttct
   124201 acatacttgc cattgagcaa gttgctgatt ttttccaatc gctgcgcttt tttttcgcgt
   124261 ttagaagccc agttgcggtt tggtttttcc ataattagtc atctccattt ttgatataga
   124321 aacgtggcgt catcaatacc atgttggtct agattgatcg tacggatccc tttatctaca
   124381 ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc aatcgtcaga
   124441 tccattccta attcttttgc caagtccatc atttggttcc actgcacagg gaaagctagg
   124501 ttttccaaca aatctttacg aatctcttct gcgtcctgag tggctcttcc atccgtattt
   124561 tttatataca aacatctagc atcagataga tggacagact gcatatacgg cttgaatttc
   124621 ttgaccgtat cattcatcaa gagacagtga gatggcacag ggactttcag cagcttcgct
   124681 gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc gatactccca
   124741 gataacgtat gttgcatcgg acaattttca tttgacagat aaactggatg gtcaggatca
   124801 aatgtttttg ctacaatttc ctcagcttcg cttctcgtca cacctactac tacgcccatt
   124861 gcataaccgc ttggataagc ttctttcatt aagactgcgc ggtgatagac tagttccgct
   124921 gcattctcaa atctcaaaga tcgacaagca acagctgcac aaaaagcacc aatcgaatgt
   124981 cctgctgcaa tatcaggctg aataccgttt ttttccattt catctaaata aaatactgct
   125041 tttgtcagta atgctagctg gataaaaacc gtatcttgat agttttcttc tttatcaact
   125101 aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca tttcagggat atccgctaac
   125161 atccctaact tctgactgcc ttgtccagga aataagaaac ctgtactcat gagcctcgcc
   125221 tcctcagcac ttactttttc cactgctatc ttagtcaatt tctgctataa taaaaaatgc
   125281 caaaaaaaga aaaaaccata acaaaaaggt atggatggga tggataatta tgaaattgct
   125341 tcatctgcag tattttaaaa aagtattcga aacaaaaaat gttacccaag cagccaaaga
   125401 attatttatt tctcaacctg ccttgagccg tgcgatcaaa catttggaaa acgaattagg
   125461 tgtccctttg ttttaccata gcggcagaaa cattgagacg accgtttatg cagaagaatt
   125521 ttatccatat gcagtcaaga cattagacac attagaagaa ggaatcaaat tattgtcttc
   125581 ggtcaatgag aagatcgtta cttccgtcat attgtattta gaggtagcct ctgtttctat
   125641 tccgaacttg gtccgaattt ttatggaaaa gcatcctgat atccaattaa cgatcatgca
   125701 acatcattta ccagatgata ttaccgatcc tgtactgtac atcacttcgg agatgaagcc
   125761 tggcatgacc aatattccta tcatgaaaga acctgtttat gtcgccattc ctaagaaaca
   125821 tcctcttgct aaaaaagaac atctgcaatt agaagatatc caacgcaccc ccatcttgat
   125881 gctgtcaaag agcaacgcct tccgtcatac actggatgca gcacttgaag cgaaaagtat
   125941 cgaattgacg atcagttcga cgacagatga tcctgcgaca ttacgttcta ttgtcaagca
   126001 aggattagct gttagctttt tcccaaaagt ttcttggtct tacgataaaa atgatccttt
   126061 cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca atttatttat cttctagctt
   126121 cacagaagat catcctttga tcaaaatgat tgcaaaaacg atgcgggaat tttatttagg
   126181 aaaataaaaa aaaaagagct gggacaaaat tcttgtcgca gctctttttt cgaacaaacg
   126241 gtgttccaga agtaatttct tagaaaataa gccgaaattt cccaaaaatt tgagaagcaa
   126301 ttttcgtaaa ttccttctta tttcttgaag ctaaacattt ctgtcacaac ctcttctcta
   126361 ttgtcctaga cattcattgc gttgatttaa aacttccatc aatcaaatct gctagttcat
   126421 ctagcttttc tattttacgt agatcaatag agatccaatg ttctttattc atgtgatagg
   126481 ctggaaaata cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg accttcaaat
   126541 caagaatatt gatgatccta tggtcacttg catcatcata gattttttct tctggaacag
   126601 acatcaggac cccaaaccat tttttgcttt tttgatgtct gaatacgcaa taatcaggaa
   126661 attttttaaa aatatattct ggagacaaat tgtatgtttc attgatgtga tcgattagtt
   126721 cttttttcgt tagcatctgc ttccatctct ttcttattga ttcgctttat tcaaagatac
   126781 cacgtaagaa tagaaaaatg aatacaagta gttgctaatc aaatacttcg atatttttct
   126841 tttttatcaa ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca tctactatta
   126901 aaatgtttat tattgtttgc ccacctattt tttgatttta gtggtatcat ttttcaagtt
   126961 catgatgaaa ttcttatcat cagtaaatgc tccagaaaaa taaacaatga ttgaaaaatc
   127021 agttgaaact tgaccaccgt ttattttctc cgtattctta gaatagaagg gagtctgatt
   127081 gatgtattca ctaatgaaaa aaatcattac cgaaaaagat attcgacgtc atgtttcttt
   127141 agtagaacaa ttgctgaatc atacaaagat aaccgtaaat gaattagctg aaatcatagg
   127201 gacaaccgaa cgaacgattt tttctgacct tcaatctatt cgctctcagc tgcccgaagg
   127261 ctgggacatc atcagcgatc aagcaggaat cagtttgcaa aaccagcaaa atttattaac
   127321 aaatgacctt tgggagattt ttttcaaaca atcagtcagt gtcgaactgc tgaaaaatct
   127381 gctttttaca aaaaaagttg ctgtccctga ttttctagcc gaccatgggc tttcttatgg
   127441 gacattgaaa agacatgtga ctaaaatcaa tcagcgttta gcaagttacg acttgcagat
   127501 cgatttgacg aaatatacag cttgtattct tggaaaagag cgtgcgattc gtacttttta
   127561 tcatcgattg ctgattcctt tcactcataa caactatttt ttcgaggatt attcgatcca
   127621 tgaatcccat tattttcaat ttttaagaaa cctcagtcaa actgaactag cggttgaaac
   127681 agaagagatt ttcggaacat gttggttctt tatcaatacg atccgtataa aagcgaattg
   127741 ccgattagat tcttctatcc ataccaacag cacactctcc tccctgtatg actctgcatt
   127801 gaaaaaattg tatttaacgg aaggtattta tcttaaagac actgagcttt cttttgcttc
   127861 tttctgcttt ctggaaagtt ggaattacaa caataactat ggacaagaaa ccgctcgctg
   127921 cctgcatcat tctccatttt tagaggtcct cgaaacgttt gtagaggaac tagcttcaga
   127981 gctgtctctt gatcaactaa aaaaaacatc tctgactgat aacttggctc tcctcatatt
   128041 aaaatatcat gaatcgccga tattgattga agcattggac cgtcaatacc atcgtttttt
   128101 agaaacatat gatcagcaat atgcacactt gtatcaacag aaagagaatt tactgaacga
   128161 tctaaaaaaa gaaatagaaa tcaatgaacc aaattacttt ctccaactct tgtctctact
   128221 gatccaacaa acgatttttt ctatcaaacc gaatttgttc aatgtctatt ttattttcca
   128281 aggagaacct tcatggaagg cgttcttgca gcaggaatta aaagattact taggaaaacg
   128341 tgtctgcttc ttacctatcg aactaaccga tctttccact atttcttttg aaaaaacaga
   128401 tatcttgatt tccaatttcc ctctagattc tctagatatc cctattgtat acatttcttc
   128461 tattccaacg aaaaatgagc tcaatcgttt gactgaactc actttcaaaa cttttttata
   128521 gtaacggcta gcgactgtat tctctagctg ttttttcttt tttgcgaaac agtttgttca
   128581 tgatcccttt ttctatttct acaactaaga atagaagaat cacatttgct ccaatcaata
   128641 actgttgtgt catcgttaaa cttactgtac cgatcagctg ctggccgact ggtagaaaaa
   128701 taaccatccc ttgcaataaa gcaaggatac ctaatgaaag aaaaagcgct ttgttttgca
   128761 aaagaccttg gttcaatgac ggcttggaca actctctgca attgatcatg taaacagctt
   128821 gagctaagac gatattttgc agaagaatgg tttgctgtaa gctttgccct tcaaactgca
   128881 tagccaataa ataggcagga accatgatca gtagggagac atagatgatc cgaaacaaat
   128941 gataaccaga taggattcct tgctttggat ctcttggtgg gcgcttcatg atttctggat
   129001 ctggcgactc gaaccctagt gcataagata aagtgattgt cgttaccata ttgacccata
   129061 aaatctgtac tggggacaaa ggtagcgggc gattcagcat aagtgcccaa atcaagatca
   129121 atccttgagc taatgcagtt ggcaagaaga aattgatggt tttttgcaaa ttatccaaaa
   129181 tccgccggcc ctcttttacg gcttttgcaa tcgtatggaa attatcatct cctaaaacca
   129241 tatcagctgc ctgtttgctt acttcactac ctttgattcc catagcaatt cctacatcgg
   129301 atcttttcaa agcaggtgca tcatttacgc cgtctccggt cattccgacg atttctccat
   129361 ttttctgcaa agctttaaca attcgcagtt tatgttctgg tgtcgttcga gcaaagacat
   129421 cgactattgg cacttgctga atcaactctt catcagacat aagatccaaa tcgatccctt
   129481 ctaaaacttt ggcagtatgt tttaagccaa tttgttccgc aatcgctttt gctgtgtctt
   129541 tgtgatcccc tgtaatcatt ttcactgaga tccctgcttg ttggcattcc tttacagctt
   129601 tgactgcact ttcttttggc ggatcgatca atccggcgat ccctgcaaaa gttaattggg
   129661 ttaaagtctc atgcgtgagt tcctgtttcg aatctacttt tttataagca aatcctaaaa
   129721 cacgctgacc ttttctcgca aattctgcag ctgtctgact ccatgcttct tgttctgcag
   129781 aagaaagagt tgatttagca aataatactt ctggtgcacc tttgacgtaa atcaccgctc
   129841 ctttttcctc cgcatgcatc gttgccatat atttgtattc agaactgaac ggaatctttg
   129901 cttctatttt tttcaaaggt agattggctg tttctgtgtg tcgaatcagc gcaacttctg
   129961 tcggattgcc acttaaattt tctgttttct gttcttcttt tgtttttaat tcctgacagt
   130021 ttttcataat ggacaacacg caggcttctt cttttgcagc tacatccaca actgtcattt
   130081 cattttttgt caatgttcct gtcttatctg aacagatcac tgtcatagaa cctaatgttt
   130141 ctaccgaagg aagtcctttg atgatcgcct tttcttttgc catttcatgt actccaacgg
   130201 atagaatcat ggtcaaaaca gctggcaatc cttcgggtac cattgacacc accaaagcaa
   130261 tcgtagccga aagaaggata tgccaatcca taccatatcg aaatgtcgta aaaataacta
   130321 agaataggct caaacaaaga atgccttgaa aaatttgttt gtttaatcgg ttgatttttt
   130381 taatcagagg cgtcgtttgt tgttcgaccg attgcaatgc ttgattgatt ttaccgattt
   130441 ctgtcgtgtt tcctgtttct acaacgacac cgactgcaga accagattgt acaagcgttc
   130501 ctgaaaaacc catatttttc tggtcgccga tcgattcttc ttctgtcaaa acggcatgac
   130561 ttttttcaga tgcttctgat tctcccgtca aaatcgattc ttccactaat aaatcatgtg
   130621 cttcgattaa tcggatatct gctggaacga catctccggc acgtaagaca atgacatctc
   130681 cttttaccag ttctttcgca gggatttttt cttgcatccc gtttgatagt actatcgctt
   130741 cttgtcccat cagctgcttc aaaccattca atgattcttc cgcttttctt tcttgccagt
   130801 aactaacgag tccattgacg atcactacga ataaaataat acttccttca atatattcac
   130861 tagaagcaaa tttcaaaaaa gaggcaatga tcaacacgat catcagcaga tcagtaaaat
   130921 gctttgctat ttttttccat cgttttgtct gctgccttgc ttttatttca ttatatcctt
   130981 gatcagtcag gcgtttctgt cgttcttctt gcgacaagcc ttctgttgat gtttgatagt
   131041 ttttcaatac atcttctacc gtcaatctgt accagttcat cctatcccct cctgtttaat
   131101 tgatactgtt attatacgca gggaagtttt ttcaggcgta tcgattattt caggggggtc
   131161 tgaaataatt cgttttgcaa aatgtatctg ggcctaactg atatttctat tgcttattct
   131221 tttatataca aaatttaaga atacaaacca gataaccaat aaaaaaggaa taaagcgtgt
   131281 cgtgctttcg aataacaagc tgaaagcaat tgaagtaaaa aacaccaaca aagcaagatc
   131341 tttatatctt tgtgtagtgt tccgtttctt ttctttaata aattgaacat gtgcacacaa
   131401 gatcgaagac cacacaaata gaaaacaact cgttgtcatg cctgccaaaa gctgaaaaaa
   131461 caatcgacta tcagctacaa aaaaatgtag acaaagaccg gcaaaaaata acattcctgt
   131521 aattaaaata ccgtgtaaag gtacgccttt cttagaaagc gcacaaaacc aatggggcaa
   131581 acttttttct tttgctgttt gatataaaat cctgctagta ctgtagactg cactatttgc
   131641 tgaggacaaa gaagcaacca aaatcattag attgacaatt gctgctccgc cttggcaatg
   131701 gatcacttgc agcatagtca ctaaaggact ttcttttaaa tctaaacaat gccatggaac
   131761 aacaaataaa atcgacaaaa tcgttcctat atagaaaaag agaatacgcc aaggcagaca
   131821 gttgattgct tttttcaaat tcttttgcgg atcacttgtc tcgccaacgg ttaagccgat
   131881 catttcaatt ccagcaaaac tgaataaaac cataggaaat gagactacca ttccagcaaa
   131941 gccaaacggt agtaattcat cgatctgtcc aaaattttct ttgattccga tagactgggt
   132001 atgaaaatct gagacactca atcctatacc caataagata aaaaggaaaa tccccaagat
   132061 cttgatgatt gagaaaaaag attccaattc accaaaccac ttgactgcaa caaggttgac
   132121 tgccattaag atcattagtg cgctacctcc gcttatcaaa gctggtacat gtacaaacca
   132181 gaaatggata tacaatccta ttgctgttaa ttccgcaagt ccagctccaa gccagcaaag
   132241 ccaataagtc caattgacaa aaaatgccca ctttttgcct aaataatagt caacgaaatc
   132301 actgatagag ttaaatgtac tatttgctaa aaataaatca cccatggccc gcatcaaacc
   132361 ataaatagcc aaaccgcata tcaaataaac gagaataaca gctggccctg ctgatgcaat
   132421 tgcctttgct gaacctaaaa ataatcctgt tccaattgcg ccacctaatg cgattaactg
   132481 gatatttcga ttagttagag tccgtttcat tgccggtttt tccatcaagt tcaccttttt
   132541 ctttttcaag cagcatggcg acttcctgtt cccaagaagg tttctttatt ttttcaatac
   132601 agagtggtac tacaaacaaa atgacactga tggctgtgac tgcaattaga ttgatccatg
   132661 ttccagcttt catcgtcagt ccgacagaaa atccgattgc cgcaaataaa acaataacga
   132721 cagtccaaac tccaagattg ctctttttct ctttttttcc aattctaaat ggtcgattca
   132781 actttgcatg tgtataacgc aatctcaaaa ctgcgatccc catgatgaca tagacgatac
   132841 agtagataac agtagttgaa tttacgagca tcaaaaatgc ttgattgacg ccaggaatca
   132901 gcaaataaaa aagtgcaaag attgagataa tgacagactg agtcaaaata acggatttgg
   132961 atacgtcaaa ttgattcgtc ttccaaaaat tgaattttgg tggatacgca ccttttctag
   133021 cactttctgt cactgtcttt gctggtcctg aggcccaagc agataattgg actgctacac
   133081 caatgaagac aagtaaacta aacagattca caataagatg tggcaaacca agaatttggc
   133141 aataaatcga aatagattgc gcgatattat tcaattccat ttgtcctttt ggtacgacat
   133201 ttgctacaac taaagcattc aaaatattga cgaaaaatat aaaaattaac gcggcaaata
   133261 ctccgcgaat atacgtttta actggatttt ccaatcgagt gatatacaca gaggacattt
   133321 cgataccagt aaaaataaac atgatcggcg caaaataaac aaatgaggat gcagtcgtag
   133381 tatctggtat taatttgtcc catgaaaaac tgcctaatgt tccggttggt acgatcccaa
   133441 ctttgatcca cgcagcaaac cctaataaga gcatcatcgt caaaggcacg tacaatccta
   133501 accaaacacc gacttttccg ccgatttttg ccatatcaaa tttcaaattc aaaaaagtga
   133561 tgatccaata aacgactaaa atgacaacta atgtaaattt gctgttcaaa ccaagcggca
   133621 cattatcgat aatattacca aacaacggag cgagtgctga agcgaccatc accatcccag
   133681 gaaacatttg gacccataac agccatgaga caacgaaccc ccattttttt cctagagcat
   133741 tcgataccca aagttctgga ccaccttttt ggggaaacat tccagcaaat tcaccagaga
   133801 tcaatgagat tggaaaagcg tacaataata ctgcaaccag catgtaaaaa atcatcgtcc
   133861 agctcgttgc tgctacatct ggaatatttc gtacacttgc tacaagggcg caggtcattc
   133921 cgataaaggt catggtcgtg atttgctttg atgaaccagt atctttcatc tttattcctt
   133981 ctttcaatag cgacatatgt ttttgttttc ttttattttt gttcaagaac agaaatgaca
   134041 aggtttcatc gattcagtac aaaacccagt atcaccgaaa catccattga acgatcttag
   134101 tgaactgggt ttgatacatg aaacgaacga tccaacttat agaacgtttt ataaaaaaga
   134161 atatcttggc gaggaatata tgaaaaacag ggggaaatac aattgttttg atgaagcaaa
   134221 taattgtctg ttctccatat gtctcttgtt attctttcac acaaaaatca gttctattgg
   134281 atgatttcgt ttagtttctt ttccattgct ttggcgattt tttttgtata aaaattgaaa
   134341 attcgatcat cattcaaata aggagtcatg atagccgcac gtagcagagt gattttctgt
   134401 tcttttttcc attcttcttt cgttagaccc attctttcaa caaaacgaat cggagaatca
   134461 ccgtaatctt cttgtgtaaa tgtagtatga gaagtaataa accgttcacc ataaacatcg
   134521 ccatccatat aagaagaccg atcgaacatt ttttcattta attcattgat agcgttcaaa
   134581 tctgtacagc cttgttcttt gaatacccaa ttgaccatat taaaatcagg atgattcaat
   134641 ggatagactt cgattgtttt tcctttaaca gtaaaagtca gatgatcgag aaattctctg
   134701 aatctttgag ctgcctcgat tgaagcccca atcaattgac catatccggt cacattcaat
   134761 ggcaatacac gatgtgctgt ccagactgcg gcggcggttg ctccagcttt tgatccttcc
   134821 aaaatataag ctcctaacat atctggagcc tttactgatt tttcaaacac atatggcgcg
   134881 aaataagaaa tgatatttct catattttta tgtttgatta cgattcctcc agcagcataa
   134941 gggacatagc ccatcttatg aggatctatc gtgacagaat ccgcttctga aatagctctg
   135001 aacccttcat atacctcttt gtcaatcgtt acgcagtggt ggaaaacatg atgttcttca
   135061 aagaattcag ctaaggaagc atacggcaca aattctcctg cttcgtttag aaacagtgaa
   135121 cgcgcatagc cgccatatgc ggcatcaaca tgtaaataga aatagatccc ttcatctttt
   135181 aacctttctc ttaattggac gattttatct acactgtcta cttgaccttc ttctgtcgtt
   135241 cctacgacag caaccacacc tagaattggg attttttgtc cagctaattc acgaattgtt
   135301 ttttccaaag cattaatatc cattcgataa tcctcttgaa ctgggatagc aaccatctga
   135361 tctaagccga ctccgcaaat atctaacgcc ttcatccaag aatagtgttt tgtttgaggg
   135421 accagccatt tccctaatct ttggatattt tttccgctac gggaagaagc tgctttgacc
   135481 tcatccattt cttcgtccgt aaagctttcg gtcatttcca aaatttcttc aacagataaa
   135541 ttcaaaagtt cccactcaga catttttttc actttttcag gcagcacttc cttgacagct
   135601 aaaggaatcg atttgatgca acgggcgtac cataatcctt caagattagc aatagaacca
   135661 tctgctgcaa tgtgtcccca tccgtctgtc atattgaata atgaagcaaa atcctgtcct
   135721 acttctgctt ccatttgcga agttgccata gaagattcca atgcaacatt atttgggttc
   135781 cacagcatcg catagttgta agctaataaa gcaggcatca atgtttcagc attcatttgt
   135841 ccccagtaac ggcctgctga atgccatggg atcgatcctg cgcgtaatcg ctgactgact
   135901 tcatccaaga cctctatcat atggtcgcgg gtggctagat aatctggagt gagcttatct
   135961 ccttcgctaa ttgcaggcat atctgaagga atataatttt ctctccatcc aagatgttca
   136021 tctaccatct tgttcaacaa cattttataa actggtccat tttcagcttt gtcgcctata
   136081 aagacggcct tgatatccat atctttcatt tttcttccct ccatattttg ttggtcaatg
   136141 tcttgcagat acatgtgttt atctgtaaca cctcttcact atagcgccaa aaatgagaat
   136201 ttttttttta taacatcaaa tccacgtaga tttttacaaa caaaaataaa taaagtcgga
   136261 tttttctgat aattcaacgc aaaaaagcga acaatttttc aataaatcag tcttctaaaa
   136321 tagataacct aaaaaaagcg aacaaacaaa taaaatgact gtccttcatt cactaaacat
   136381 atttttattt ttttatattg ttaaaaattc gcaatttatt ttttaataga attatctgtt
   136441 ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt tttcagaaat
   136501 taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata
   136561 caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg
   136621 tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc
   136681 ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa
   136741 gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat
   136801 atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt
   136861 ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc
   136921 tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg
   136981 atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt
   137041 aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa
   137101 cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa
   137161 aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct
   137221 gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc
   137281 gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt
   137341 attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa
   137401 gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt
   137461 gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg
   137521 ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt
   137581 tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc
   137641 tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg
   137701 atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca
   137761 aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat
   137821 tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag
   137881 tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa
   137941 aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac
   138001 aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt
   138061 ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg
   138121 gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta
   138181 gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa
   138241 gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc
   138301 tgactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg
   138361 atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt
   138421 tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct
   138481 ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat
   138541 aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat
   138601 atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt
   138661 atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag
   138721 tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat
   138781 agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt
   138841 tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa
   138901 aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc
   138961 cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag
   139021 tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta
   139081 aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca
   139141 ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt
   139201 tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt
   139261 ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct
   139321 gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc
   139381 gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa gacattggag
   139441 gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag
   139501 cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat
   139561 tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat taatattgga
   139621 ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta
   139681 attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc
   139741 cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca
   139801 aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata
   139861 ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac
   139921 tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact
   139981 tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc
   140041 gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca
   140101 atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag
   140161 aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac
   140221 cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag
   140281 aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag
   140341 attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag
   140401 cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat
   140461 tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt
   140521 tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat
   140581 ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctatcgtcg
   140641 tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt
   140701 tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa
   140761 aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata
   140821 ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc
   140881 cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta
   140941 aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct
   141001 ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc
   141061 ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg
   141121 ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa
   141181 taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc
   141241 agatgcaagc ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc ctgcatcaag
   141301 taataagaac acccaagcta aaactgcttc ttatgttttt gacttggttg gacttattac
   141361 cgcagatgca agcttttgaa caccatttac caagaggtta tgaaaaagct gccctgcatc
   141421 aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat
   141481 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt
   141541 ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact
   141601 taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt
   141661 tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata
   141721 caactatttc aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa
   141781 atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta
   141841 agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct
   141901 gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag
   141961 aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac
   142021 agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg
   142081 gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac
   142141 attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt
   142201 ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg
   142261 gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag
   142321 agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa
   142381 agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt
   142441 aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa
   142501 attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct
   142561 agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc
   142621 tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg
   142681 aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg
   142741 cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt
   142801 aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga
   142861 tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat
   142921 gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga
   142981 agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt
   143041 gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa
   143101 tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg
   143161 cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat
   143221 tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta
   143281 tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa
   143341 catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg
   143401 agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga
   143461 gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa
   143521 aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt
   143581 ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac
   143641 agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc
   143701 gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc
   143761 ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt
   143821 gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag
   143881 ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa
   143941 gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat
   144001 cacttgttct ccgttttttg attgatcaag catctaaagc ttcttttctt ctttcttgga
   144061 tcgtagctac cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg
   144121 ctagtacacc acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta
   144181 cccatggagg cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa
   144241 cactaactaa gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga
   144301 taggtaaccc aaacatcgtt ggttcattaa tattgaagga cacctggcgc aatcgacaag
   144361 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
   144421 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
   144481 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
   144541 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
   144601 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
   144661 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
   144721 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
   144781 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
   144841 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
   144901 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
   144961 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
   145021 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
   145081 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
   145141 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
   145201 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
   145261 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
   145321 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
   145381 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
   145441 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
   145501 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
   145561 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
   145621 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
   145681 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
   145741 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
   145801 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
   145861 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
   145921 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
   145981 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
   146041 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
   146101 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
   146161 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
   146221 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
   146281 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
   146341 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
   146401 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
   146461 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
   146521 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
   146581 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
   146641 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
   146701 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
   146761 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
   146821 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
   146881 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
   146941 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
   147001 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
   147061 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
   147121 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
   147181 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
   147241 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
   147301 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
   147361 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
   147421 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
   147481 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
   147541 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
   147601 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
   147661 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
   147721 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
   147781 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
   147841 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
   147901 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
   147961 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
   148021 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
   148081 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
   148141 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
   148201 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
   148261 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
   148321 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
   148381 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
   148441 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
   148501 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
   148561 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
   148621 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
   148681 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
   148741 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
   148801 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
   148861 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
   148921 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
   148981 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
   149041 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
   149101 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
   149161 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
   149221 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
   149281 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
   149341 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
   149401 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
   149461 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
   149521 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
   149581 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
   149641 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
   149701 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
   149761 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
   149821 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
   149881 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
   149941 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
   150001 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
   150061 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
   150121 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
   150181 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
   150241 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
   150301 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
   150361 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
   150421 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
   150481 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
   150541 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
   150601 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
   150661 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
   150721 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
   150781 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
   150841 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
   150901 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
   150961 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
   151021 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
   151081 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
   151141 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
   151201 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
   151261 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
   151321 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
   151381 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
   151441 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
   151501 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
   151561 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
   151621 taattctttt tgtcgttcgc cttgataaaa aatcactgga cgatattgct tccctctatc
   151681 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
   151741 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
   151801 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
   151861 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
   151921 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
   151981 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
   152041 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
   152101 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
   152161 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
   152221 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
   152281 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
   152341 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
   152401 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
   152461 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
   152521 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
   152581 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
   152641 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
   152701 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
   152761 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
   152821 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
   152881 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
   152941 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
   153001 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
   153061 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
   153121 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
   153181 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
   153241 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
   153301 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
   153361 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
   153421 aaaaatgata attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa
   153481 actcatactc aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg
   153541 ataagcataa aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga
   153601 acgatagtag atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa
   153661 tgccgctttt ttttgtttta aaaaccgctc gccataatga taagctacga gtgttgtaca
   153721 caagttcaat aaccaaacgt aaataatata gcttacaatc aaattttttg tcatccaata
   153781 taacattaca gctggaaaaa aagtcagaaa gggataaaaa taatttacac catatccagt
   153841 ataattgaat gtctcataat taatcggact ggtaaaaata gtcgataacc ccttgattcg
   153901 agataagtga aaagaaagat ctccagtggt ataaatactc tgtgtatttt tgaaaaagat
   153961 gaaagaatat atactactaa ctataattaa tccactcaat attactactt ttttatttat
   154021 tttcattctt cttgtttatc tcctttttag tcacatatcg agaatctttc aacttattag
   154081 gtgcaaatcg tttaaaactt gaatacacca ctaaacaata agtcagaata ctgatccata
   154141 aagccatgag agcagttgga gtatattttg ttgtaatttc tatcttattt tctccagcat
   154201 ttacatccac attcactaag ccatgttttt tataagagtc atttacgact ttaccgttta
   154261 cttttacctg tgtacctaaa taagcatata ctggtactgt cactttttgc tttttgttgc
   154321 ttgccactgt gtaagtaatg ttattatctg cagctttata cttccctttt tcccacgatt
   154381 ctcctacttt tacttcatgt ttttctaagt gaggcctttc ttttatcgct tctgttgggg
   154441 cgtaatcgta taatcctccc atatctgatg ttgtataacg tgggagtgtt tcttcattga
   154501 taacaatatg accgtgtttt gattggataa cttttgcgta attttctttt gctgctatag
   154561 acgtgacgag tagaaacacc acagtagcgc caatgattaa ccatgaatat tttttattca
   154621 tagtcttttt cataaaaaca agcgttatga tcattgaaga aaaaaagatt tggataccta
   154681 agatacgcca aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa aaccatggaa
   154741 ataaattagt tacgcatacc catgtagaaa ctacgccaac atagatgatt ttttcttttt
   154801 tctccatttc tttcagtatg aataaaggaa aaatcaacgc aaccaatcct aataacccca
   154861 tagtgtatgt tgtaatatcg ttatttaacg cttgaatgat actatctgaa atagaaagtg
   154921 cttctagctg caaatctgtt ttgaagggac tgtgtatttc ttgataattc atctgctgca
   154981 acataggata ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc tttactaaat
   155041 tcacgaccac aaattttata tcgtttttta tttgcttgct taaaaacaaa cgaactataa
   155101 tcatacaagc aatcaatgca ctacacaata gtgtagttaa gacatgagta tacacaagta
   155161 gacttatact gacagataaa gcgatgactt ttttttctct attgaatatc aatccataag
   155221 cataatataa aacagctggg ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat
   155281 aatagatatc aactgttcga taagttgcaa aagtatataa acaactaaat aaaaaagcag
   155341 catctatctt tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DAFFFQ010000002        92612 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000002
VERSION     DAFFFQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.002"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            160..840
                     /locus_tag="LQB69_000137"
     CDS             160..840
                     /locus_tag="LQB69_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3524333.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="LQB69_000138"
     CDS             930..1964
                     /locus_tag="LQB69_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL3524334.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="LQB69_000139"
     CDS             1978..2313
                     /locus_tag="LQB69_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524335.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="LQB69_000140"
     CDS             2297..4777
                     /locus_tag="LQB69_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524336.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="LQB69_000141"
     CDS             4833..6392
                     /locus_tag="LQB69_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524337.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="LQB69_000142"
     CDS             6389..7753
                     /locus_tag="LQB69_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3524338.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="LQB69_000143"
     CDS             7924..8994
                     /locus_tag="LQB69_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524339.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="LQB69_000144"
     CDS             9009..9677
                     /locus_tag="LQB69_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524340.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="LQB69_000145"
     CDS             9697..10275
                     /locus_tag="LQB69_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3524341.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="LQB69_000146"
     CDS             10676..11587
                     /locus_tag="LQB69_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3524342.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="LQB69_000147"
     CDS             complement(11965..12882)
                     /locus_tag="LQB69_000147"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL3524343.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="LQB69_000148"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="LQB69_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL3524344.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="LQB69_000149"
     CDS             13579..14127
                     /locus_tag="LQB69_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL3524345.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="LQB69_000150"
     CDS             14242..16602
                     /locus_tag="LQB69_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL3524346.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="LQB69_000151"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="LQB69_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL3524347.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="LQB69_000152"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="LQB69_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL3524348.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="LQB69_000153"
     CDS             19108..20007
                     /locus_tag="LQB69_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524349.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="LQB69_000154"
     CDS             20000..21262
                     /locus_tag="LQB69_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524350.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="LQB69_000155"
     CDS             21423..23321
                     /locus_tag="LQB69_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL3524351.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="LQB69_000156"
     CDS             complement(23651..24370)
                     /locus_tag="LQB69_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL3524352.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24706..25038
                     /locus_tag="LQB69_000157"
     CDS             24706..25038
                     /locus_tag="LQB69_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524353.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25025..27028
                     /locus_tag="LQB69_000158"
     CDS             25025..27028
                     /locus_tag="LQB69_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL3524354.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27047..27517
                     /locus_tag="LQB69_000159"
     CDS             27047..27517
                     /locus_tag="LQB69_000159"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL3524355.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27538..28122
                     /locus_tag="LQB69_000160"
     CDS             27538..28122
                     /locus_tag="LQB69_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL3524356.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28137..29123
                     /locus_tag="LQB69_000161"
     CDS             28137..29123
                     /locus_tag="LQB69_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL3524357.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29113..29424
                     /locus_tag="LQB69_000162"
     CDS             29113..29424
                     /locus_tag="LQB69_000162"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL3524358.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29446..31227
                     /locus_tag="LQB69_000163"
     CDS             29446..31227
                     /locus_tag="LQB69_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL3524359.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31220..32596
                     /locus_tag="LQB69_000164"
     CDS             31220..32596
                     /locus_tag="LQB69_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL3524360.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32603..33238
                     /locus_tag="LQB69_000165"
     CDS             32603..33238
                     /locus_tag="LQB69_000165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL3524361.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33268..34623
                     /locus_tag="LQB69_000166"
     CDS             33268..34623
                     /locus_tag="LQB69_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL3524362.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34815..36209)
                     /locus_tag="LQB69_000167"
     CDS             complement(34815..36209)
                     /locus_tag="LQB69_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL3524363.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHFQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36301..37272
                     /locus_tag="LQB69_000168"
     CDS             36301..37272
                     /locus_tag="LQB69_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL3524364.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37269..38051
                     /locus_tag="LQB69_000169"
     CDS             37269..38051
                     /locus_tag="LQB69_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL3524365.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38220..38591)
                     /locus_tag="LQB69_000170"
     CDS             complement(38220..38591)
                     /locus_tag="LQB69_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3524366.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38725..39168
                     /locus_tag="LQB69_000171"
     CDS             38725..39168
                     /locus_tag="LQB69_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL3524367.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LQB69_000172"
     CDS             complement(39211..40020)
                     /gene="yidA"
                     /locus_tag="LQB69_000172"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL3524368.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40039..41406)
                     /locus_tag="LQB69_000173"
     CDS             complement(40039..41406)
                     /locus_tag="LQB69_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3524369.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEYIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41390..42220)
                     /locus_tag="LQB69_000174"
     CDS             complement(41390..42220)
                     /locus_tag="LQB69_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL3524370.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFTLTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42383..42820
                     /locus_tag="LQB69_000175"
     CDS             42383..42820
                     /locus_tag="LQB69_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL3524371.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42896..43516
                     /locus_tag="LQB69_000176"
     CDS             42896..43516
                     /locus_tag="LQB69_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL3524372.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43705..44211
                     /locus_tag="LQB69_000177"
     CDS             43705..44211
                     /locus_tag="LQB69_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL3524373.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44228..45127
                     /locus_tag="LQB69_000178"
     CDS             44228..45127
                     /locus_tag="LQB69_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL3524374.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45214..46098
                     /locus_tag="LQB69_000179"
     CDS             45214..46098
                     /locus_tag="LQB69_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL3524375.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46290..46361)
                     /locus_tag="LQB69_000180"
     tRNA            complement(46290..46361)
                     /locus_tag="LQB69_000180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46326..46328),aa:Arg,seq:ccg)
     gene            46421..47755
                     /gene="rpoN"
                     /locus_tag="LQB69_000181"
     CDS             46421..47755
                     /gene="rpoN"
                     /locus_tag="LQB69_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL3524376.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47904..48995
                     /locus_tag="LQB69_000182"
     CDS             47904..48995
                     /locus_tag="LQB69_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL3524377.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49036..50037
                     /gene="gap"
                     /locus_tag="LQB69_000183"
     CDS             49036..50037
                     /gene="gap"
                     /locus_tag="LQB69_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL3524378.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50147..51337
                     /locus_tag="LQB69_000184"
     CDS             50147..51337
                     /locus_tag="LQB69_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL3524379.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51441..52196
                     /locus_tag="LQB69_000185"
     CDS             51441..52196
                     /locus_tag="LQB69_000185"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL3524380.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52312..53610
                     /gene="eno"
                     /locus_tag="LQB69_000186"
     CDS             52312..53610
                     /gene="eno"
                     /locus_tag="LQB69_000186"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL3524381.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            54010..55149
                     /locus_tag="LQB69_000187"
     CDS             54010..55149
                     /locus_tag="LQB69_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL3524382.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEELTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(55216..55791)
                     /locus_tag="LQB69_000188"
     CDS             complement(55216..55791)
                     /locus_tag="LQB69_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3524383.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(55796..56770)
                     /locus_tag="LQB69_000189"
     CDS             complement(55796..56770)
                     /locus_tag="LQB69_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL3524384.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(56794..56838)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            57053..58020
                     /locus_tag="LQB69_000190"
                     /pseudo
     CDS             57053..58020
                     /locus_tag="LQB69_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            58133..58411
                     /locus_tag="LQB69_000191"
     CDS             58133..58411
                     /locus_tag="LQB69_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL3524385.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCTYAKKEFEKKPEYQDVLRQS"
     gene            58529..58765
                     /gene="secG"
                     /locus_tag="LQB69_000192"
     CDS             58529..58765
                     /gene="secG"
                     /locus_tag="LQB69_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL3524386.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            58868..59623
                     /locus_tag="LQB69_000193"
     CDS             58868..59623
                     /locus_tag="LQB69_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3524387.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            59626..61983
                     /gene="rnr"
                     /locus_tag="LQB69_000194"
     CDS             59626..61983
                     /gene="rnr"
                     /locus_tag="LQB69_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL3524388.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            62005..62469
                     /gene="smpB"
                     /locus_tag="LQB69_000195"
     CDS             62005..62469
                     /gene="smpB"
                     /locus_tag="LQB69_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL3524389.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            62771..63490
                     /gene="atpB"
                     /locus_tag="LQB69_000196"
     CDS             62771..63490
                     /gene="atpB"
                     /locus_tag="LQB69_000196"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL3524390.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            63543..63758
                     /gene="atpE"
                     /locus_tag="LQB69_000197"
     CDS             63543..63758
                     /gene="atpE"
                     /locus_tag="LQB69_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL3524391.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            63849..64373
                     /gene="atpF"
                     /locus_tag="LQB69_000198"
     CDS             63849..64373
                     /gene="atpF"
                     /locus_tag="LQB69_000198"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL3524392.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            64360..64902
                     /locus_tag="LQB69_000199"
     CDS             64360..64902
                     /locus_tag="LQB69_000199"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL3524393.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            64925..66481
                     /locus_tag="LQB69_000200"
     CDS             64925..66481
                     /locus_tag="LQB69_000200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL3524394.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            66495..67397
                     /locus_tag="LQB69_000201"
     CDS             66495..67397
                     /locus_tag="LQB69_000201"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL3524395.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            67420..68826
                     /gene="atpD"
                     /locus_tag="LQB69_000202"
     CDS             67420..68826
                     /gene="atpD"
                     /locus_tag="LQB69_000202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL3524396.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            68840..69262
                     /locus_tag="LQB69_000203"
     CDS             68840..69262
                     /locus_tag="LQB69_000203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL3524397.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            69731..69961
                     /locus_tag="LQB69_000204"
     CDS             69731..69961
                     /locus_tag="LQB69_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL3524398.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            70058..71359
                     /gene="murA"
                     /locus_tag="LQB69_000205"
     CDS             70058..71359
                     /gene="murA"
                     /locus_tag="LQB69_000205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL3524399.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            71372..71548
                     /locus_tag="LQB69_000206"
     CDS             71372..71548
                     /locus_tag="LQB69_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL3524400.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            71683..71979
                     /gene="yidD"
                     /locus_tag="LQB69_000207"
     CDS             71683..71979
                     /gene="yidD"
                     /locus_tag="LQB69_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL3524401.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            72163..72630
                     /locus_tag="LQB69_000208"
     CDS             72163..72630
                     /locus_tag="LQB69_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524402.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            72715..73599
                     /locus_tag="LQB69_000209"
     CDS             72715..73599
                     /locus_tag="LQB69_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3524403.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            73744..73950
                     /locus_tag="LQB69_000210"
     CDS             73744..73950
                     /locus_tag="LQB69_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL3524404.1"
                     /translation="MRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGPKEYEIFFGE
                     EDFGKEKSLENAIETVIRHWNLHE"
     gene            74038..74118
                     /locus_tag="LQB69_000211"
     tRNA            74038..74118
                     /locus_tag="LQB69_000211"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74072..74074,aa:Tyr,seq:gta)
     gene            74125..74196
                     /locus_tag="LQB69_000212"
     tRNA            74125..74196
                     /locus_tag="LQB69_000212"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74157..74159,aa:Gln,seq:ttg)
     gene            74321..74491
                     /locus_tag="LQB69_000213"
     CDS             74321..74491
                     /locus_tag="LQB69_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524405.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            74711..75418
                     /locus_tag="LQB69_000214"
     CDS             74711..75418
                     /locus_tag="LQB69_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3524406.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(75478..76122)
                     /locus_tag="LQB69_000215"
     CDS             complement(75478..76122)
                     /locus_tag="LQB69_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL3524407.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            76249..76536
                     /locus_tag="LQB69_000216"
     CDS             76249..76536
                     /locus_tag="LQB69_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524408.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            76644..76964
                     /locus_tag="LQB69_000217"
     CDS             76644..76964
                     /locus_tag="LQB69_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3524409.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            77045..78358
                     /locus_tag="LQB69_000218"
     CDS             77045..78358
                     /locus_tag="LQB69_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL3524410.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            78355..79074
                     /locus_tag="LQB69_000219"
     CDS             78355..79074
                     /locus_tag="LQB69_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL3524411.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            79202..79753
                     /gene="raiA"
                     /locus_tag="LQB69_000220"
     CDS             79202..79753
                     /gene="raiA"
                     /locus_tag="LQB69_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL3524412.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            79975..82509
                     /gene="secA"
                     /locus_tag="LQB69_000221"
     CDS             79975..82509
                     /gene="secA"
                     /locus_tag="LQB69_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL3524413.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            82635..83736
                     /gene="prfB"
                     /locus_tag="LQB69_000222"
     CDS             join(82635..82706,82708..83736)
                     /gene="prfB"
                     /locus_tag="LQB69_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL3524414.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            83841..84527
                     /gene="ftsE"
                     /locus_tag="LQB69_000223"
     CDS             83841..84527
                     /gene="ftsE"
                     /locus_tag="LQB69_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL3524415.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            84511..85404
                     /locus_tag="LQB69_000224"
     CDS             84511..85404
                     /locus_tag="LQB69_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524416.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            85556..87004
                     /gene="cls"
                     /locus_tag="LQB69_000225"
     CDS             85556..87004
                     /gene="cls"
                     /locus_tag="LQB69_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL3524417.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            87341..88198
                     /locus_tag="LQB69_000226"
     CDS             87341..88198
                     /locus_tag="LQB69_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL3524418.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            88313..89233
                     /gene="pstC"
                     /locus_tag="LQB69_000227"
     CDS             88313..89233
                     /gene="pstC"
                     /locus_tag="LQB69_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL3524419.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            89233..90117
                     /gene="pstA"
                     /locus_tag="LQB69_000228"
     CDS             89233..90117
                     /gene="pstA"
                     /locus_tag="LQB69_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL3524420.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            90132..90938
                     /gene="pstB"
                     /locus_tag="LQB69_000229"
     CDS             90132..90938
                     /gene="pstB"
                     /locus_tag="LQB69_000229"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL3524421.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            90954..91712
                     /locus_tag="LQB69_000230"
     CDS             90954..91712
                     /locus_tag="LQB69_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL3524422.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            91725..92402
                     /gene="phoU"
                     /locus_tag="LQB69_000231"
     CDS             91725..92402
                     /gene="phoU"
                     /locus_tag="LQB69_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL3524423.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT        30685 a        16126 c        19804 g        25997 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaatta
    24421 aactatgtaa gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag
    24481 aaatttcgtg tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa
    24541 agtaagcaga aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga
    24601 gaaggacatt ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt
    24661 caatgaaaaa agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta
    24721 gaagctttgg aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg
    24781 cggcagcagc tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca
    24841 aactatcgac aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa
    24901 ttagaaaaag aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa
    24961 gatcaatatc aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa
    25021 acttatggcc gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga
    25081 gaggatttta caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa
    25141 tagaacaaac aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga
    25201 agcaaaatta agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat
    25261 acaacaccac ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt
    25321 acaagatttg gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct
    25381 tgatttaaaa gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga
    25441 agaatggctt cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac
    25501 gaccattttt gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact
    25561 gagtaagctt gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct
    25621 ttcgtatgtt gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg
    25681 gttcaataaa gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa
    25741 gcagcaatta tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg
    25801 cagtggctat atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg
    25861 agaaaaaata aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt
    25921 agatgcagaa gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga
    25981 tatatacgtc agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa
    26041 attgaaaaac catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc
    26101 aaaatatgaa gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg
    26161 gatgatggtc gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca
    26221 aaaactactt gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc
    26281 gataccatcg atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa
    26341 agaactgttt ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac
    26401 gattttgatt ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc
    26461 agcaaaggaa aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg
    26521 gcaagggatc ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac
    26581 agcatttgtt taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt
    26641 tccgattatc caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta
    26701 cggactgacc ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat
    26761 ttcaggcgga agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc
    26821 tcgattcagt gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac
    26881 actgctaagt gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt
    26941 ttatacagga ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat
    27001 caatcacaag aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt
    27061 aattagtcaa aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt
    27121 ttcagggatc ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac
    27181 aacaagtcaa cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca
    27241 aggtttgtat ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc
    27301 agttgtacaa ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt
    27361 ttcaggtatt gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc
    27421 tgaacatgct acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg
    27481 tttcgttatt tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg
    27541 aacgccattg aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg
    27601 ttagaacaag aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt
    27661 gaaacagaac atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt
    27721 cagctaagga atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa
    27781 tttttaagac gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa
    27841 caaatccagt ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc
    27901 attgctggag aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat
    27961 gaacttccct ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg
    28021 ataaacgatc aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa
    28081 ggaacgatga gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg
    28141 aataccacga actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga
    28201 catttgaacg gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca
    28261 cgatttacag tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa
    28321 aggagcagag ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt
    28381 ggatctatac gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga
    28441 caaatcaaaa tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga
    28501 aggatgccat tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac
    28561 gagaagtacg tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg
    28621 atcgacagtt tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc
    28681 tgacagaagt cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat
    28741 tgcagaaaag aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa
    28801 aagaagtatt tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag
    28861 agacagatta tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa
    28921 agcttgaaca gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg
    28981 ctttcggacc actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc
    29041 ttcgtttact ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg
    29101 tgagacaagt ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca
    29161 ttcaaattgt ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca
    29221 attgaagaaa tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca
    29281 ttgagcccag aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt
    29341 cccaatcatc aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa
    29401 gcagtaggtc aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa
    29461 atcataaaag tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa
    29521 gacatgtgtc tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat
    29581 gtggcgtcta ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc
    29641 tctactggtg aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac
    29701 ggaatccaac ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc
    29761 ggagttcaat tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa
    29821 gccggagaaa cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata
    29881 aaacataaaa tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt
    29941 tcattcacta ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg
    30001 acgatgcttc aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg
    30061 gatgcaccca tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga
    30121 ggagcagcag ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt
    30181 gcaaaatggg ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag
    30241 atgacggatg ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg
    30301 atggaacgta cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct
    30361 tctatctata cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct
    30421 atcatggcag actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg
    30481 gaagaaatgc ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac
    30541 tatgaacgat ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc
    30601 gcaatcagtg ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca
    30661 ttgcgggtcg taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt
    30721 ccttcgatcg attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg
    30781 gatcatatcc ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag
    30841 gaagaacagc agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat
    30901 gaccgtctga cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct
    30961 tttgatgatg tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc
    31021 ttgacctttg gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc
    31081 atggacggta ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca
    31141 gaattagaaa aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt
    31201 tcagaaggag gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc
    31261 attgatggcg gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat
    31321 gcaaaatggc gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt
    31381 ccaaatattc gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg
    31441 ccatccttta gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg
    31501 acgaccgaaa gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga
    31561 agtaatcaat ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc
    31621 aatcgatcat ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg
    31681 tcttcctcat aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc
    31741 agatgatttc gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt
    31801 catggaagat tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc
    31861 aaacgaccct gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata
    31921 tcttgcttat gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc
    31981 agaggcattg cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc
    32041 tgggtatctt tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact
    32101 aaaaggatcg gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc
    32161 aatccctgac ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta
    32221 caagagtggg attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa
    32281 gggaactgga cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc
    32341 agcctatgca caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt
    32401 atctgatgta gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa
    32461 tcaaggattc tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct
    32521 atcgatgttg ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct
    32581 gccagaaggg aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt
    32641 gactcgattg aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa
    32701 gcaagatgaa ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca
    32761 agaaatggaa gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat
    32821 cacggaacct ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat
    32881 cgtagacaaa aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca
    32941 gctgacagac acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc
    33001 aatcgatcgg tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac
    33061 ttgtcaactg atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata
    33121 tatgacgatc cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa
    33181 cgagcgcgca gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt
    33241 ggtatctttt tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt
    33301 ttgccggctg ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc
    33361 ctacttaccc taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt
    33421 ttcactgcaa cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat
    33481 tggaacagtg ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt
    33541 atgatggttc cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt
    33601 attatattaa aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt
    33661 tacatcctaa aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta
    33721 tttgtgcctg aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct
    33781 agtttttgta atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat
    33841 gtttatttga tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta
    33901 tggcgtgatt tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca
    33961 ttaaccgtta caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc
    34021 aatggggcaa cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg
    34081 agtgtgacac ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca
    34141 ggtctgattt taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc
    34201 ggattgaaaa caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga
    34261 cgtacgagag cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt
    34321 actttatttt ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact
    34381 gaaacgatac ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt
    34441 ggtacagtag gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc
    34501 attatagcgc ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg
    34561 acaaaagcac aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga
    34621 taatttcatc ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt
    34681 tcttgcttct tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt
    34741 tgttgaacct cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
    34801 tatttatcaa taggttataa ttcatatttt tttatttttt cttttacgat acttagtcca
    34861 gaagctaaaa tttcaaggtt attagaggat agggaaattc gaacgaatgc atctggttgc
    34921 gcacgtgtag taaagcgtct ggaatgataa agtcggatac cgttagcaag aaagtcattc
    34981 tcaattatct tagggtcaat ctcaggatca actggaagaa ttcgatagta agggacacga
    35041 ttcgttggtt tttctacttt gaaaagagaa tcaaaaagct gattggctct ttttgtaaga
    35101 cttaatttag tttctaagat cctatttgct tcatttgaat ggaggatttg agtgatgact
    35161 tccacatcta atccagaagt tttcacattt atattaaaaa ttgcttgttc tatcttgctt
    35221 tggaaatgta ctggaactac aagataagca actcgcaatc cagaacaaat aaattttgtc
    35281 attccagcta agtagattgt ttggttaggt aataaatttt ggaaaggagg aatgatcgta
    35341 tgttgatagt aagttgttaa aaatgaatgg atatcatctt caataaccca tagaccttct
    35401 ttttcaatca cttcggctaa ttgctctcgt cttttgtgag agatttgaaa ccctatagga
    35461 ttattacaag aaggcataag aaagacacca tgaatcttct tttttctaca ttcctgaatc
    35521 aatatatcag gtcgcattcc ttgctcgtca aaatcaattg gaacgatctc taaatgatat
    35581 aactgagcca attcaatgaa attagcgtat gtatatcggt ctgttgctat tctatcacct
    35641 ggagagaaaa gtgcagaaag tgtgatagcc aatccatttt gaacaccgga aacaatagat
    35701 agagattcta aggacggaat gactccttga gtttttaacc aatttgctgc agtagtcaat
    35761 tgaaaatttg tgcctttggg atcttggtaa ttaagcaaac caacaagatt tgggttatta
    35821 ctagttgatt gaataaacgg gacaacgaat ttattacatt cttcaaaaga actaaccaga
    35881 ccaaaatcaa tgatatgttc ggttacttca ttggtagaga tggtgatgga ctcaaagaca
    35941 ttgggagaaa taaatgtgcc acttccaata ttcgtataca gcaaaccttt ctcaattcca
    36001 tatttataag ccttgccaac ggtagtaaaa tttaaatcta aataatcagc tagctctctt
    36061 tgggagggta actgagtgtt tttctgtaat ttattcttta aaatatcttg ttctaattgt
    36121 tgaatcaacg atcgataata aggacgcttc aatttttgtt tgtctggtcg ccaagttaac
    36181 gggtaattgt cataagaatt aataggcatt ttctccactc cttaaaattg tataccatac
    36241 aattttacca ttgattgtat tgattttaga agttaatctt ttgtgaggag ggaattgtct
    36301 atgaaaatta ttaattttga ggaagtagta gcttctttta gttttgaaga agcgattcaa
    36361 gtaatgaaaa aatgtttttt tgattatgaa agtggtaaaa tcagccaaaa tcctcgattg
    36421 gtggaaattt tacctgacgg aaaaggcaaa aatatgtttg cgatgatgcc tgcttatttg
    36481 ggggagaaca gatattttgg cacaaaagtc attaccgctt ttccggataa tcatcagtat
    36541 catttacctt ctcatttagg agagattttc ttgtttgatg ctcaaaacgg actacccaaa
    36601 gcattgataa acgcaaatgc agtcacctgg attcgaacag ctgctgtatc tgcgctggca
    36661 acagattact tagcaaatcc gcatgcagaa acacttgctt taattggagc tggtcaacaa
    36721 gcattgtcgc atttgactgc gatttctacc attcgtccca tcactacagt gtttgtttac
    36781 gatattttag aagaaagacg aaatactttt atcgctaaag ctaaaaaaca gtatcctaaa
    36841 ataacgttta taaatgcaat atcagtagaa gatgcagtac gtccagcgga aatcatttgt
    36901 acgttgacct ctagtaaaga ggcatttctt caaaaagagt gggtaaggga agacgcacat
    36961 atcaatgctg tgggcacatt tactcctgat acaagagaga taagtacatc gttgatggaa
    37021 acttctcaaa tctatgtaga tgattactca gctgccttaa cagaaagcgg ggacctgctt
    37081 atagccatgg cagaaggaga tttagaggaa tcttgtattg ttggtacatt aggcgaatta
    37141 gtttctgaga aaaaaacatt ttataaagga gaatcgaggc cgacgatttt cgacgcagtt
    37201 ggtctagcta ttgaagatct ttgttgtgca gaatatattt ataacaaatt agagaaaggg
    37261 gctttaaaat gaacttagat tattacgtag ttgatgcatt tgcagatgag gtgttcaaag
    37321 gaaatccagc agcggtttat gtgctagaag aatggctgcc agaaggtacg atgcaaaaaa
    37381 ttgccattga aaacaattta tctgaaacag catttacagt gaagaaaaac caagaatttg
    37441 agcttagatg gtttacgcca gatcgagaaa tcgatctttg tggtcacgcg acattagcaa
    37501 cagcatttgt tttatttaac tattacaaaa tacctgatga aacgattaaa ttttctactc
    37561 aaagcggtaa tctatttgtg acaagaatta aagattatta ttacatggat tttcctagta
    37621 ttatgccgaa aaaagtgccg atattgtctg aatatgaaga agcgattggg gctaagatta
    37681 aagaagccta cttagcacgt gatttatttt tcgtattaga agatgaaaaa acggtggcta
    37741 aattgcagcc agattttaca gctatcaaaa attttgaatt aggaatagga gtcatcgtga
    37801 cagcagaaag tgtgcaaaaa gactttgttt ctagaacctt ctttccaaaa ttaacaatca
    37861 atgaagatcc agtatgtgga tcagcacatt ctaatcttat tccttattgg gcagaaagaa
    37921 tgaataaaac aaaattgtca gcttatcaaa tgtcatcaag gggcggggat ctagaatgtg
    37981 aattaaaaga agatcgtgtg ataataggag gaaaagcagt tttgttttct aaaggtacct
    38041 cgtttatttg atttctttca aatgaattaa aaataaattg gtggagagtg gcaaccttat
    38101 agctacaatg gttgttaaaa aaagacgcaa actttttcaa tttaatgaaa agtttgcgtc
    38161 tcattttaat tattggtgtt tgttttttat tcaatcaaga gtaaaatgcc ccatactaac
    38221 tatcctcgga tgacttcgat tttataacca tcagggtcaa tgacaaagta gtaagaaggc
    38281 gcagtgccag gtaatccttt taagtcagta atctcaagac cagcagcttt atgttgctcg
    38341 tgtaattttt ctagatcatc agctgctata gcgatatgac catagccgtc tcctagatca
    38401 tacgcaccat gatcatagtt gtaagtcaac tctaattcat agtcatcccc aggaagtgtc
    38461 aaataaacaa gtgtaaattt gttttctgga aaatcacggc ggcggctttc ttcaaaacca
    38521 aatgcctttt gataaaattc gattgatgct tcaagatctt tcactcgaac acatgtgtga
    38581 gccattttca tagattcatc tctccttttc cttttactca atcataacaa aaagaagtaa
    38641 ggaaatagtt gatttgtgct tgttcatgag aaatttctca tgtaaaatgg atagaaaata
    38701 aaactgttaa ggtggaaact attcatggat aaaccaattt ttggtgaaaa aaagcaattt
    38761 gctgattatc aaacccgtta tgcagcatat attgtcatag aaagagatca gcagattgca
    38821 gtaatcgagg ctccaaatgg tgcttttttt cttcctggcg gggaaattga aggagaagag
    38881 acgaaagaag aagcgattgt ccgtgagctg atagaagaaa tgggaattgt ggcagaaatt
    38941 tcttcttatc tgggagaagc tgatgagtat ttttactcga attatcgtgc aacttattat
    39001 tataatccag gatatttctt tgttgctgat tcttggaagc gtataggaga accaactgaa
    39061 aagaccaata aaatttggtg ggtgagccct caagaagcgc ttgtgaaatt gaaacgtggt
    39121 agccaccgat gggcagtcga gagatggatc gaaaaaaata aaaactgaaa gaaagcgagt
    39181 agatcagctt tctctcagtt tttttattta ttacagtaca tattttttga ttgcttcagc
    39241 tacaccgtgc tcgtcgtttg atgaagtgat gacatttgct gcttctttga caagagaaac
    39301 ggcatttccc attgctactc ctagaccagc atattcaatc atggataagt cgttctcgtt
    39361 atctccgata gccatgattt catcttgatc aataccaagg tgctgtgcga gattcgcaac
    39421 tgccgcacct ttacttgctt ctttatttag tacttcgaag taatagggcg cacttttgac
    39481 cgttgtgtat ttatcacgga aagtttgcgg taaacgcgca attgctgcat ctaaaatttc
    39541 tggttcgtcg atcatcatca ttttgacgat gttcatgtct ggtgtcattt cttcaggtgt
    39601 tcgatatttt agcggcatct tgactaaact tgcttcgtgg atcgtataag cgctgatatc
    39661 tcgattaggc gtgtagatcg ctttgtcatc gatcgaatgg agatgactgc cgacacggcg
    39721 tgccattact tcgatctcta ggaaatcttc atgtgtcagt ccgtgacggg cgatgatttt
    39781 acctgatttc gtttgttgta cgagtgcccc gttataagta ataacgtaat cattatcttg
    39841 aaaaaggttc aactcgttta actgttcctg gacacctggt aagggacggc cagtgcataa
    39901 aacgatcgaa acaccttttg caacagcagc ggcaatcgct tcttttactt cttttgttac
    39961 ttctcttttc tcatttaata aagtaccatc gatgtcgata gcgactagtt tgatagacat
    40021 aatttcctcc aatatatttt aatttatttc aatcaatgcg ccattacgga tatggctggc
    40081 aaattcttga taagtttcgt caaataaatc ataatgatca cgcaaggacg gatcaaccat
    40141 ttcttttgga aagaaaaatc gctcatctcc ttgttcttgt cccgctaatg cagcaacaag
    40201 ctgactaacc tgtgataatt ctatcaaagt tccgtcattt cgcaaaatct cgatttgtgt
    40261 gcgatgtctg ccttgttctg ggcgataaaa atcataaggt agatcgtagc tagaattcac
    40321 agcagtgtaa taaacaggat tatatccgac cttttctact aacaaagtga gttcttggac
    40381 taattttgaa tcccggttgc cagaaaaagt agcagatttc agtggtttac gattcaaaaa
    40441 acgtttagct aaatcattta aaataggatc agcagattca gaccattgag taaagtaagt
    40501 gcccaagacc ccatcatcaa gtttgaggta ttcttctaaa gtaaagcttc cgtcaagaaa
    40561 tggcagaagt aattgagaat ggagttcaaa actgctggca tcttcttgat acaactcgtg
    40621 tgcgcgatga agcagatgct ccaaaattac ttccatacca cgggaaacag gatggaagta
    40681 cacttgcaca tacatttgat atcggctaac gatataatct tccactgcat gcatgccact
    40741 catggaaaaa gcgatcccgc cttcataagg acgaatcaca cgtaatattc gggtaagatc
    40801 aaatgttcca tattctgttc cagtgaaata agcatcccga agcaaatagt ccattcgatc
    40861 tgcatcgatc tgactagaaa tcatttggac aacttgtggg ttggggtaag ttttttggat
    40921 cacactagct accttttcag gaaaaccttc ttctactcgg ttaagaatct gatacacttc
    40981 tgtttctgga gaagtgatga tctctacagt gatggcttca tgattcgtat ggaagatata
    41041 ttcaaatgta tgagagtaag gaccatgacc gacatcatgc aacagtgccg cacataaagt
    41101 caccagccgt tcctgatcat cccagcctcc gttgcctatt ttttcttttg aaaaatttcg
    41161 agaaaagata tcgcagattc gtcttgaaat ctcataaacg cctaatgaat gagtaaaacg
    41221 gctatgttct gctccgtgaa aagtgaaaga agaagtgcct aattgtttaa tgcgtcgtag
    41281 acgttggact tctttcgaat tgatcaaatc taagattact tgatgctgga catggatata
    41341 gttatggact ggatcacgaa aaactttttc cataggcagc atttgatatt tataaggggt
    41401 tgtcataatg tagctccttt atgttattac gttgttccat tctctggatt tggttgttcc
    41461 attgctccag ctggatttct ttttttagcc agtcggtaga atcctgtaaa tcacttgcag
    41521 aatacagctt ttcaaaagct tttgcaaatg cttctttgac ttcttttaca aaaaggggtt
    41581 ggtcaagcag ttcttcaagt gttgccattg tagcaggatc cactgcagga tagccatttg
    41641 taccaaagtc ttcttttaaa gcagattgat aaaatcgctt catgacttgc ccacgttttt
    41701 cctgattacc gttgacactt aagtacatca taacagaaat cccatttttg atccgacgct
    41761 gagcgatacc ggcaaatttt ttgccatgga tgcttaaatc ataagttcca ggacaataag
    41821 aatgggtgat ttcgaatgct tgtatcggtg cctttggaaa agcgtattta gtcaatagga
    41881 gcatttgctc gtaagcgtca tctatggtta ttttttttgt gtcactttgg gggaaaatca
    41941 aagaaatatt caaaattcct tcatctgata cgatccctaa tccacctgaa tttctgatca
    42001 ctggagcgta acctgcatct tccagtgttt ggatcccgtc acttagaaaa ggagttcgaa
    42061 catccttcat acctaagatg actgtgcagt catattgcca gaaatgtaaa aagatttgct
    42121 ggtgttcttt agaatattcc gttaatatat ctgttaatgt aaaaggggaa aagtcgtttt
    42181 tttgataaat gtgctgatct agtcgaatcg cagggttcat tcaaccggct cctttgtata
    42241 aattcatatt ttctacaata ttatactcta aaatcaaaga gttgttttgg aaaaaaggtt
    42301 ttctgtaaaa atggacaagt tggttgacaa aacaaaagaa actaggaaca atgtagctat
    42361 gagaagtaaa ggagaatgaa taatggaatt atcacaagga acaccgattt caatcaaatt
    42421 gcgtacaaaa gtaaaacagc aaggggaagt gcaagatttt tattttgatt tgaaagggca
    42481 aatggtgaca attggcgata cgctgtacat acgttataaa gagattcagg aggagacggg
    42541 cgatgagatt cctgtaacga tcaaattagt tccagatggt catgtacagc tgatccgtgc
    42601 aggagaaatg cggatgcgtt tgaaatttgg ctataaagaa cggttagaaa catcctatcg
    42661 aacaccatac ggtatgatcc agattgctac ttttacgaaa gaactgcatg ttagtttgaa
    42721 agaccgtccc acagcaggga aggttcgaat cgattatgat ctgttcatgg gaccggaaaa
    42781 aatcggcgaa tattatttga ccttggattt tacagcgtaa atataaaaga aaagtttgat
    42841 tttttgtgtc tgattttgta tgataaggag tagactgtga aaggacgtgt cctgattgga
    42901 aattagtgta tttgaaggtg caaacaaaag cgaattatca atgatcgaag tagcacacgc
    42961 gatcttagaa caacgtggcg atgtcatgga tttttctgat ttagctaacc agatccaaaa
    43021 ctatcttggg aaatcagaca gtgaaattcg tgattcactc gcacaatttt atactgactt
    43081 gaatattgac ggcagcttta tctcattagg ggataatcgt tggggcttgc gttcatggta
    43141 tgctatcgat tcgatcgatg aagaagtaaa ccatggaatg gacgaagagg atgaagatac
    43201 tccacgtcgc cgtaaacgca agaaagtcaa cgcctttatc aatgatgatg aagacgcgat
    43261 cgattacaat gacgatgacc cagaagatac agatctgaca gaagaagatg acgatgatct
    43321 gtttgatgac gatgatgatg aagatgaaga aatcgctgct tataattctg acttgcaaga
    43381 aatcggagca gatgacccga cagatgatga agaagacttg ccaggaaata tcgaagaaga
    43441 tttaagtatc atagacgaag acgatgatga tgaagattat gaagaggaag aattttctga
    43501 ctctgaaaaa gaataaacat tattttctta cgagaggttg tgacagagac gatcagccta
    43561 tcagaataag gaggtgtgac acgatttttg tcacaccttc ttttttgtat ataaaaatcc
    43621 caagtaaata cataaaaaat aaaaaaattg aaacttttag cgcaccttgt acgtcatatt
    43681 aagtgaggca gaaaggagtg gagaatgatt tttactggat ataagaaaaa acggcagatc
    43741 gaaaaatacc gtcacctgct gaatggactc atcgaaactg attatcaaaa aatgttccgt
    43801 atcacgatga attacgtaca tgataaagaa gaggccttag atgtgatgca agatagtttt
    43861 cataaagccc tgaactattt tgaaaaagaa aaagagatcg agcattttag tgcatggttt
    43921 tatcggatat tgatccgcac ggcattagat agttggcgca gacaaaaaag gacgcctgtg
    43981 gacttatttg aaattcaaga tatcccccaa cagttacctt tagaaacttc cgttgcagag
    44041 ctccatatga ttttagataa aatagacagt ccggaaaaag agattctgat cctctacttt
    44101 tttgaaggat ttcgtttgaa agaaattgcg actatcttgg atatgaatga aaacacagtt
    44161 aaaacgaaaa tgtaccgctc attgaatgct ttacgtcaaa tcctagaatg aaggagagaa
    44221 gagtgccatg tattcccaaa aagaaaattt aaaaaaacta aagaaaactt ataaagaaca
    44281 agcaattccc aaagggctta aagaggagat ccgccagcgt tttattatcg aagagcaatc
    44341 atttataaaa gcaaagcgga gaaagagacg tctccaaggg ttcggagtat ctttggctat
    44401 cgtaatgatt acaacaagta gccttctttt taatagtcaa gttcgaagct ttgcagaaga
    44461 tttacccatc ttaggatcag tcattgaatt gatcttaggc gaacgcttta ctgatcgatc
    44521 tgaaaaaatc gatattcagg taccgaagat caatacacaa aatgagaaag aaaataaaac
    44581 aattcatgga ttaaatcaaa aatactttcg cgaaggacaa gcagattttg aaaaagctga
    44641 aaaagaatat ggaaattttg aaacggatca ttaccaagtt ttgggagatt atcagaaaat
    44701 cgtagacgat aaccggtttc tcgtgattga aaggcagatc acccaaacag cagcagatag
    44761 ccatgttgaa aaaagatacg acacaatcga caagaaaaac agtgtccagc tctcacttcc
    44821 acttttattc aaagatgaca cctatctatc tgttttgaca aaagaagtca aacggcagat
    44881 ggccgaacaa gtcaaggaag atccatctaa gtactattgg actgagcaag atattcaaga
    44941 agggacgata gaaaaaccaa cacttgttac gcctacacga agtttttatt tgaacaaaga
    45001 gcatcaatta gttttgactt tttctcaata cgaaattgct cctggttata tgggtacacc
    45061 agaatttgtc attcctaagt ccgtgacaaa aacgattttg gcatcggaag attatttgga
    45121 tcattagacg agaagtagtt gcgactagca gtttcaattt tcaagtaagt acaagtaaat
    45181 aataagaaga gaaaaagaga aagaagagat atagtgaaaa gaaattattt agttatcagt
    45241 attattgctt tatccgtgct tttgtttttc gaaattactt cggtggcctc cttgatcacc
    45301 gaagtaaaag gacaaacaca ctcttcggag cattcgtcac aatctttcaa acaatcagaa
    45361 caatcaacca ttaaaagcca atcaactgaa acagctaacg tcaaagaagc cattcaagaa
    45421 tcagcggaaa aggtacaaag tcagtcatca caatcaacac aagacagtac aaaaacgagc
    45481 cgagatccgg ctgtcaaaag cgtcgcaatc agctttgatg atggaccagg agcgaccaca
    45541 acaccgcagc ttctgcgaat cttaaaagaa aaaaacgtgc atgccacttt tttcgtctta
    45601 ggagaaaata cggcgcagca tcctgaaatc gtgaagcaga cagctgaagc agggcacgag
    45661 attggcaatc acacatacga tcatcagaat ctggccactc tttctgccca gtcgatgacg
    45721 gaggaagtga cgaaagcaga cactgagatc aaaaaagtca ctggcaaaac ccccactttt
    45781 gttcgtccgc cctatggttc tgtcacgaat gtaggagcaa cgatcatcca gcgaccgatc
    45841 atcgaatggt ctgttgattc agaagattgg aagacaagaa atcctgattt gattttgcag
    45901 aagatccaag caacagtata cgatggggca attattttat tccatgatat ctatcctgaa
    45961 acgattcgtg ccgttcctca agtgatcgat tatctgaaag agcaagggta tcgcatcacg
    46021 acagttgggg atttattagg gcatccaacg gctgtcgaga actattatgg cagaaacgat
    46081 catcggccag ttcagtaagt gagacaagta tctttaataa ataacacatt ttaataagta
    46141 gttgaacaca taactacctt acttgcaaag gcttaacaat atctatacag gtacaacaaa
    46201 aaactgtcca ccaattttct ttggcggaca gttttcactt aaaaataaag aagcaactat
    46261 tcgtaaatta tgctaaaata gcagtagaac gcgtcctgag ggaatcgaac ccccgtctca
    46321 agaaccggaa tcttacgtga tatccactac actaaggacg caagcactgg gataattgta
    46381 actaaacaga taaaaaatta caaggggatt ggacaaaaaa atgaaatttg agcaaaattt
    46441 ttcacaaaaa cagcagcaga cacagaaact agccatgacg caacagttgc aacagtccat
    46501 ccaagtttta caatataaca gtgaggagct gttggcattc gttgagaatc aagcaatgga
    46561 aaatccattg gtggaagttg ttgagccgga atggcagcca gattacataa aagcttcttc
    46621 atcttcttat gaaggagaag agacaaatta cctaaatcag attcctgata caaaagggtc
    46681 tttatttgat tcattgatcg aacaagtcca tttgaattac cgagatactt ttttgaggaa
    46741 aatcgttctt tatcttgtag aatacatcga tttgaacgga tttttgacga ttagtttaga
    46801 agaagccgga aaagagattg gagctacgcc tatccaaatg ctcgatgcgt tgactctgat
    46861 tcagcaatta gaaccagctg gagtaggtgc acgtagtttg caagaatgtc tcatgcttca
    46921 aacagaacga gatgattatg cgccggaatt agcttatatc gtgttggaag aatgttttga
    46981 ggaactggtg gagcgaaaat ggaaagaaat cgcacaacgt ttttctgttg atctacatgc
    47041 tgttcagcag atattcgact atttacaaac gttgagtcca agccctggcc ggattttcga
    47101 ccgtagcagc gaattgtaca ttattcctga tgtccgggta ttagtggacg acgataaaaa
    47161 tgtacaagtt atctcaaacc gaaaaaatca gccaaatata cgctttcaag agagttactt
    47221 caaacaaatg tcacaacaag cagataaaga aactgaaaat tatttaaaag aacggaaaca
    47281 ggaattcgaa tggttgaaga aaacgatcct acaacgtggg gatacaattt tgcgggttgc
    47341 tcaagtcatt gtttcccggc aaaaagcttt ctttatcgac aaggaacgtc caatcaagcc
    47401 tcttacgtta aaggaagttg caactgagat agatgtgcat gaatccacgg tcagtcgtgc
    47461 agtcaacggg aaatatttgg aaacagactt cggtgtattt gaattaaaaa aatttttcac
    47521 tacacgaatt cctacgaata gtacagaaca gacagaagac ctttcagcag atacagcgaa
    47581 gaaaaagctg caggaattag tcgatcaaga agacaaaaac aagccgttgt ctgaccagaa
    47641 attggttgaa ttgctgaaaa aagatgagat agcgatctca agacgaacag tagcaaaata
    47701 tcgtgattta ttaggaatcc cgagttcatc aaaaagaaaa cgttatgata actaatcagt
    47761 caaaagacta gaaaaagtat ttcacttttt ctagtctttt tagttgaaaa acgagagcaa
    47821 aactgttaga cttacagtcg tgaggttgag atttcccttt attgatgtga aattcgaata
    47881 gttaatttct ttttttatcg tatatgggtc gttttaagtc tacattggac gtaaagtgtc
    47941 caactaagga gatcgctatg ctggatgaaa taaaaatgat tgaatctgtt gcaccagata
    48001 ttatcgaagt gttgcaagaa cgctataaaa ttttacgaaa tatctattgg atgcagccga
    48061 ttggacgtcg aagtttatcg gaaagtatgg ggatcacaga acgagtattg cgaacagaaa
    48121 cagatctttt gaaaaatctg aatttgatcg acacctcaaa aagcggtatg acgctgacaa
    48181 ctaagggcga agaagtttat caaagccttg aaaacttcat ggatcagctt ttaggtacgc
    48241 atcaaaccga acaacagttg gcagaatatt ttggtaacca acgctgtatc gttgtttcag
    48301 gaaacagcga agagcagacc aaagtggctg atgcattcgg cgaagcgctg agcgaagccc
    48361 tagaccgact acttccagaa ggcgagaata tcatcgctgt gatgggagga actactatgg
    48421 ctgtggtagc cgaacaattg tctaatctgg aaaataagaa acggcataac ctgtttgtcc
    48481 ctgcaagagg cgggattggt gaagcgataa ctgtacaggc aaattctgtc agtgcaagaa
    48541 tggctgctaa agcaaacggt aaccaccgtg cactttatgt tccggaacaa ttaagtctgg
    48601 caacttacaa ttctttactc aatgagccat ctatccaaga agtcttaaac ttgattagtg
    48661 aagcgaattg tgttattcat agtattgggc gtgcattaca catggcagca cgtcgtaaga
    48721 tgactgaaaa agaattagtc atgctgaaac aagcaaatgc tgttgcggaa tcttttggct
    48781 actttttcaa tgaaaaaggt gaagtcgttt ataaggtccc tagaatcggc atagaattgc
    48841 aagatttgga aaaagtcccg atcatcatgg caatcgcggg cggaaaatca aaagccaaag
    48901 caattcgagc ttatatgaaa aatgcaccaa aacaaacgtg gctcatcact gatgaagctg
    48961 ctgcaaatga gattttaaaa ggggtaaccc tttaaaataa atattttttt gattttcata
    49021 aggaggaaat cttaaatgac agttaaagta ggtattaatg gttttggacg tattggacgt
    49081 ctagcattcc gtcgtatcca agatgtagat ggaatcgaag tagtagcaat caacgacttg
    49141 acagatgcta aaatgttggc acacttgtta aaatacgaca caactcaagg acgtttcaac
    49201 ggaacagttg aagttcatga aggttcattc aacgtaaacg gaaaagaagt taaagtatta
    49261 gctaaccgca acccagaaga attaccatgg ggcgaactag gcgtagatat cgttctagaa
    49321 tgtactggtt tcttcacttc taaatcagca gctgaaaaac atttaacagc tggtgctaaa
    49381 cgcgttgtta tctctgctcc tggcggaaac gatgttccaa caatcgttta taacacaaac
    49441 cacgaaacat taactggtaa agaaacagtt atctcaggtg cttcatgtac tactaactgt
    49501 ttagctccta tggctaaaac attgaacgac aaatttggtg ttgttgaagg attaatgaca
    49561 actatccacg cttacacagg tgaccaaatg actctagacg gacctcatcc taaaggtgac
    49621 ttccgccgtg cacgcgctgc tgctgcaaac atcgttccta actcaacagg tgctgctaaa
    49681 gctatcggtt tagtaattcc tgaattgaac ggtaaattag acggagctgc tcaacgtgtt
    49741 cctgtaccaa caggttcatt aacagaatta gtaacagttc ttgaaaaaga agtaactgtt
    49801 gacgaaatca atgcagcaat gaaagaagct tcaaacgaat cttacggata caacacagac
    49861 gaaatcgttt cttcagatat cgttggtatg acttacggtt cattatttga tgctacacaa
    49921 actaaagtaa tgacagttgg cgacaaacaa ttagttaaaa ctgttgcttg gtacgacaac
    49981 gaaatgtcat acactgcaca attagtacgt actttagaat acttcgctaa cttataagat
    50041 agacaaacgt acaagtgtaa attatagaca tgtacattta aaagcgggga agcaatcgcg
    50101 cttcttccgc tttttttgat aataagttta ttcaggagga aataaaatgg ctaagaaaac
    50161 agtgaaagat atcgatttaa aagacaaaaa agttcttgtc cgtgttgact tcaacgttcc
    50221 tttgaaagac ggcgtgatca ctgacgacac tcgtatcaaa gcagcattgc caacaatcaa
    50281 ctacgttcta gaacaaggcg gaaaagcaat ccttttctct caccttggac gtgtaaaaac
    50341 agaagaagat aaagaaggaa aatctttagc tccagttgca aaacgtctag gcgaattatt
    50401 aggtaaagaa gtaacattcg tacctgaaac tcgcggagaa caattagaag aagcaatccg
    50461 caacatgaat gacggcgacg ttgtcgtatt cgaaaataca cgatttgaag atattgacgg
    50521 taaaaaagaa agcaaaaatg atacagaact aggtaaatac tgggcttcat taggcgatgt
    50581 atttgttaac gatgctttcg gtacagctca ccgtgcacat gcttctaacg taggtatcgc
    50641 ttcaactggt atcccaacag ttgctggatt cttgatggaa aaagaaatca agttcatcgg
    50701 tgaagcagta gaagaaccaa aacgaccaat gattgcaatc cttggtggcg caaaagtttc
    50761 tgataaaatc ggcgtaatcg aaaacttgat tcctaaagca gataaaatct tgatcggtgg
    50821 cggaatgact tacacattct acgaagcaaa aggaatcaag atcggtaact ctctagtaga
    50881 agctgataaa gtggaactag caaaagaatt aatcgaaaaa gcaggcgaca aacttgtctt
    50941 gccaatcgac aatgtctgtg caccagaatt ttcaaatgat gttgaaacac aagtaatcga
    51001 aggagacatt cctgatggtt tgatggcttt agacatcggc ccagcatcag taaaattatt
    51061 tgctgataca ttacaaggtg caaaaacagt tgtttggaac ggacctatgg gtgtatttga
    51121 aatgagtaac tttgctaaag gtacaatcgg tgtttgtgaa gcaatcgcta acttagaagg
    51181 cgcaacaaca atcatcggtg gcggagactc agccgcagca gcagaacaat taggatttgc
    51241 tgacaaattc acacacatct caacaggtgg cggcgcaagc ttggaattgc ttgaaggtaa
    51301 agaattacca ggactagcag caatcaacga taaataaacg accaagtttg ctcaattctg
    51361 acgagtaatc gatcaaaagg tgaagggagt tttcatgact ccctagccat ttttcaataa
    51421 attaaggagt gtgttcctca atgcgtaaac ctattatcgc aggtaactgg aaaatgaaca
    51481 aaactgcatc agaagcaaaa gaatttgctg aagcagtaaa aaacaaaatc ccttcaaatg
    51541 acaaagttga ttcagtcatc ggatctcctg cattattctt acaagaatta atgacatcag
    51601 ctgaaggtac agaactaaaa atctcagcac aaaactgcta ctgggaaaac tcaggtgcgt
    51661 tcactggtga aacttcacca gcagctcttg ctgatctagg tgttcattat gtgatcatcg
    51721 gacattcaga acgtcgtgaa tatttccatg aaacagacga agacatcaat aaaaaagcga
    51781 aagctatctt tgcaaatggt atgacaccaa tcctatgctg tggcgaatct ttagaaacct
    51841 atgaagcagg caaaactgct gaatggatcg aaggacaaat tacagctggt ttagctgatc
    51901 tttcagctga acaagtgtca aacatggtta ttgcttatga accaatctgg gcaatcggta
    51961 ctggtaaatc agctgacgca caaatcgctg acgaaatttg tggagttgta cgtcaaacag
    52021 ttgaaaaact ttatggaaaa gaagtatcag aagcagttcg tatccaatac ggcggttctg
    52081 taaaaccaga aaatattgct gaatacatgg caaaagaaaa tgttgacggt gctttagtcg
    52141 gtggcgcaag cttgcaagca gattcattct tgtcattgtt agacgctgta aaataattgt
    52201 tgaaaatttg cgaaaaatgc aagaaatgtt tgcactaatg agcaaaatta actaaaatcg
    52261 tatatgaggg aaatccctta ggaaaaaaca aactcaaagg agagacaaaa catgtcaatt
    52321 attactgatg tttacgctcg cgagatctta gactcacgtg gtaacccaac aatcgaagta
    52381 gaagtataca cagaaagcgg agcttttggc cgcggaatgg ttccttcagg tgcttcaact
    52441 ggtgaatacg aagcagttga attacgtgac ggagacaaag ctcgttatgg cggtaaaggt
    52501 gtaactaaag ctgtcgacaa cgtaaacaac atcattgctg aagcaatcat tggctacgat
    52561 gttcgtgacc aaatggctat cgacaaagct atgatcgctt tagacggaac tcctaacaaa
    52621 ggtaaattag gtgcgaacgc tattttaggt gtttctatcg ctgttgcacg tgcagctgct
    52681 gactacctag aagtaccttt ataccactac ctaggcggat tcaacacaaa agtattgcca
    52741 acaccaatga tgaacatcat caacggcgga tcacatgctg ataactcaat cgacttccaa
    52801 gaattcatga tcatgcctgt tggcgctcca acattcaaag aagctctacg tatgggtgct
    52861 gaagtattcc acgcattagc ttcaatcttg aaagctcgcg gattagcaac ttctgttggt
    52921 gacgaaggtg gatttgctcc aaaccttggt tcaaacgaag aaggttttga agtaatcatc
    52981 gaagcaatcg aaaaagctgg ctatgtacct ggtaaagacg ttgttcttgc tatggatgct
    53041 gcttcttcag aattctacga caaagaaaaa ggtgtttacg tactagctga ttcaggcgaa
    53101 ggcgaaaaaa caactgacga aatgatcaaa ttctacgaag aattagtttc taaatatcca
    53161 atcatctcaa tcgaagacgg attagacgaa aacgactggg acggattcaa gaaattaact
    53221 gacgtattag gcgacaaagt tcaattagtt ggtgacgact tgttcgtaac aaacactcaa
    53281 aaattatctg aaggtatcga aaaaggaatt gctaactcaa tccttatcaa agtgaaccaa
    53341 atcggtacat taacagaaac tttcgaagct atcgaaatgg ctaaagaagc tggctacaca
    53401 gcagttgtat ctcaccgttc tggtgaaaca gaagattcaa caatctctga tatcgctgtt
    53461 gcaacaaacg ctggccaaat caaaactggt tctctatcac gtactgaccg tattgctaaa
    53521 tacaaccaat tactacgtat cgaagaccaa cttggtgaag ttgcagaata caaaggtttg
    53581 aaatctttct acaacttaaa aaacaaataa ttcttttcta atgaagatac atgatcgact
    53641 attgaaagat agtcgagtat gatcacttag ttaagtaatc gaactctcta cttgctgaaa
    53701 tatcagcagg tagagagttt tttttgcaac tagcttctgt atatgatgtt tacaaagaat
    53761 ggtacaagaa aagatcatcc ttgaaaaagg aaagctaaca ataactagca tcatataaga
    53821 gcttttaagg atagaacagt agaataggaa tctatgagca agtcctctcg tataaataaa
    53881 ataggaggga agtagcagca tatttttcat aaagtggctc tctttcttca tttttacttt
    53941 ggttgctcct atttgaaaat tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga
    54001 gaggattaga tgaaaaaaga agaaaaaatc gcgattttac aagagatcat ccgaattaaa
    54061 tcagtgaatg gaaacgaagg tgaagtagca gcctatttga ataagctttt ggcaaagcat
    54121 gacataacag gagagattgt ttcttacaga gatggtagag ataatttgat tgctcgttac
    54181 caaaagggcc aatcaggaaa agtgttaggc ctatctgggc atatggatgt cgtggcggct
    54241 ggtgatgaat cgtcttggac ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg
    54301 tacggacgtg gtgctacaga tatgaagagt ggcttggcag caatggtgat tgccatgatc
    54361 gaactgaaag agtcaggaaa accatttaac ggaacagtca aattattagc cactgtcgga
    54421 gaagaagtgg gtgaactagg tggggaagag ctcacgaaag caggctatgt agatgactta
    54481 gatgccttga tcatcggaga gccaactaat tacagtttga tgtatacgca catgggatca
    54541 atcaactata cagtcacctc gcacggaaaa gaagcgcata gctctatgcc agaccaaggt
    54601 tataatgcaa tcaatcacct aaatgaattc attacgaaag caaatgctga aatgaatcac
    54661 ctcgctgaaa caatcgagaa tcctgtccta ggaaaaacaa tccataatgt tacattgatc
    54721 agtggcggga atcaagtcaa cagcatccca agccatgcgc agctccaagg gaacattcga
    54781 tcgattccag aatacccaaa tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa
    54841 ttgaatcaag agacagacta tcatttggaa ttgatgatcg attacaacaa aatacctgtc
    54901 aaagctgatc cagattctcc tttgattcat tgcattcagc aacaattcag ccagccatta
    54961 cctttagttg gagcggcagc aacaacggat gcagccgaat ttacgaaagc taaccattcc
    55021 ttcgactttg tcgtctttgg gcctggtgtc gttactttgc ctcatcaagt agatgagtat
    55081 gttgaaatcg ataattattt ggatatgatt gaaaaatatc aaggaattat tttatcctac
    55141 ttagcgtaag atttttttaa taaatgaaaa aacagagcca gattagcatt attttgctaa
    55201 tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt gtggcaacat
    55261 agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt ctgtacctgc
    55321 ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata gctgaacgat
    55381 catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga tcgaagaaac
    55441 gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc tgtttttatc
    55501 tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa tttggattgg
    55561 cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc tggacaatcc
    55621 tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc cgatagaatc
    55681 gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat tgatcccaat
    55741 acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca ttttgttagc
    55801 ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt aaaagaacgg
    55861 ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta ctgttagcag
    55921 gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt ccttgagttt
    55981 ccacagcagt gaagcttttg aaaccttcac agatggcccc ctcgataaaa taagcaacag
    56041 ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg aagtcaaaat
    56101 aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga cagcagtatt
    56161 cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc atttctatcg
    56221 tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag taattgtatt
    56281 cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag acaaaacgat
    56341 ccatatgttt cccaatattt gggttgactt tcaaagcaaa agcgatattg gtcaaaggac
    56401 ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa aaatcaacgg
    56461 catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc cctaaaccat
    56521 ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt tccattcctt
    56581 gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca ttctgcgtac
    56641 ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg atatcagggg
    56701 atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa tcaataatta
    56761 cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta ttctgggaag
    56821 tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag tatactggct
    56881 tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac tttaagtaaa
    56941 tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc ctatggatcg
    57001 gtacattgga taatagttag attatagttt ggcagaaagc ggcgggtgta caatggttat
    57061 tttttatttc attcttattc tgataattat agggatcagt taccattttc tttcttattt
    57121 ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt atgaagacac
    57181 tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaacg acaaaaatta
    57241 gaagaagagt gtgaaagctt ttgggagcag ggaacaccca aaacaatcaa aagttatgac
    57301 gggttgaaac tagctggtca gatgttcatc catccaaata agcagaataa atgggtcatt
    57361 tgcgtacatg attaccgaag cacagggaaa agagacatgt ctcatatagg taaaagatat
    57421 gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga aagtgaagga
    57481 gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat ccaactcatc
    57541 ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg agcttctacg
    57601 atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat tttagacagc
    57661 ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac tgtctttcct
    57721 aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg ttactcctta
    57781 aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt ggtcattcat
    57841 ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa tgctacggca
    57901 ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc gatgaaagat
    57961 ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac gatgaattaa
    58021 ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga tcaggagact
    58081 gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc atatggaaat
    58141 caaagaagaa aaaaatcgtc ttgctttatt caatgatgaa caacaagaga tcggagaaat
    58201 gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg tagatccagc
    58261 atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat tagcaagacg
    58321 agacgggaaa aagatcattc ctttatgcac ttatgcaaaa aaagagtttg aaaagaaacc
    58381 agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt tgaactttca
    58441 gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa gttaaagaga
    58501 cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa tcgtgatcgt
    58561 tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa atagtgcagc
    58621 gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc gcggctttga
    58681 agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct tattatttgt
    58741 cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc gggcggcttt
    58801 ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt aaaatgaggc
    58861 gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac atggaaagcg
    58921 agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa tgctcgcacg
    58981 atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac acggaacaat
    59041 ggagccatat gatattttgc aagaaaaagc agaaagctgg tgggcagaca ctaaaaaagc
    59101 catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat tatcaatggg
    59161 cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg gttttttctg
    59221 ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat acgtacgtca
    59281 agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag ttgcttaccg
    59341 ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg tagaaagtag
    59401 gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg aaatgatcga
    59461 ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga cacttcgatg
    59521 gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat tagaaaaaga
    59581 cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac aaaagaaacg
    59641 ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt ttctatggaa
    59701 gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact agtacaaacg
    59761 attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa agtgaaactt
    59821 cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg atttggattt
    59881 gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac tggttttgca
    59941 atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga tatggatcgc
    60001 ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt agcaggggaa
    60061 ttcgttgctt attcagaaga agaaatacaa gaaacggatc tttacggcgt tgttatcccg
    60121 aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt tcgtccagtt
    60181 gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta tgcgactagt
    60241 ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat ggatatcctc
    60301 tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt gtctgaagca
    60361 gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga tttaagaaat
    60421 cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc agttacggtt
    60481 aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt ttcttactat
    60541 gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag tgtatatttg
    60601 actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg ttcactgaat
    60661 ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa tggacaagta
    60721 gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac ctacacagct
    60781 gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc tcagctcgta
    60841 cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg aatcaaacgt
    60901 ggcgcgattt cttttgagga tcgcgaagca aaaatccttg ttgatgctga aggacaccca
    60961 aatgatatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc tttcatgctt
    61021 gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt tatttaccga
    61081 atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc ttcagctttg
    61141 ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca gcatgtgatc
    61201 gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct aagaagcatg
    61261 caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc agattactac
    61321 acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg tctgatccgt
    61381 tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctacgga acttcctgaa
    61441 attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg tgaagtagat
    61501 tccatgaaga aagcagaatt catgatggac aaagtggggg aagaattcga tgggatcatc
    61561 agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga gggattgatc
    61621 catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt agctcttgta
    61681 ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa agtagtcaaa
    61741 gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga ggttacagcg
    61801 ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa acaagcagga
    61861 aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa gcaaacgttc
    61921 ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa aaagaaaaaa
    61981 taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat tgcacaaaac
    62041 aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat ggtcttgcaa
    62101 ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg atttattcgc
    62161 gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga acaaggaaat
    62221 atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa acaaatcatc
    62281 cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa agtctatatt
    62341 cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc ttatgataaa
    62401 cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt aaaaaatttc
    62461 tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta cttaatgata
    62521 aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc ttagatcagg
    62581 agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa gtgtctaatt
    62641 ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt ggttagaaac
    62701 cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg gggaaaagag
    62761 gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt ggtttgatgg
    62821 tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag cttacttttt
    62881 tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat gggtcattga
    62941 ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca atttccattt
    63001 gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt tagtaacaaa
    63061 aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc cgtttgttac
    63121 tttaactttg gcatttataa tgatcttact tactcactta tttggggtga aaaagttagg
    63181 cttcaaagga tatttcgtaa attcgttctt gaaaccatat agttttatgt ttccgatgaa
    63241 actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg ggaatattta
    63301 tgcgggggaa gttttgctaa ccttgattgc aaaaatggta ataagcttag gctggttctc
    63361 attgccgtta gcaattccat tagagatggt ctggatcgca ttctccttgt ttatcggaag
    63421 catccaagca tttgtcttcg ttactttatc tatggtttat atgagccata agattgaagt
    63481 ggaagaataa tattcgtttt aaaaacaaac tactatttat ctaatttagg aggaaaacaa
    63541 ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga gccggttatg
    63601 gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct gaaatgtctg
    63661 gtcaattccg tacaacaatg tttatcgggg tagctctagt cgaagctgtt cctatcttag
    63721 gggtagttgt cgccttgatt ctagtttttg gtgtttaatt gaatcagtat taaggcagaa
    63781 acaatttggt ttgttactgc ttttttacat gaaaactaga agtacttcaa agaaaggaag
    63841 gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg ttaggaaatg
    63901 tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcat tttgcttggg
    63961 gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat ttggattctg
    64021 cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa caattgttag
    64081 cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga gaattaagcc
    64141 gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagt aaagcccaag
    64201 ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgat gttgcagaac
    64261 tatctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa atgcacgaat
    64321 cattaatcaa tcaatatatc gaaggtctag gttcttcaaa tgaaactaga taaatataca
    64381 gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa taaagcagaa
    64441 gaaatttatc aagatctcgt aagcttacgt caaatctatg aacaagtacc tggattaggt
    64501 aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat ggacaagctt
    64561 gttagtggct atgatggaat cgttaaaaac tttttagaag ttgtttatgc gtataaccga
    64621 atggatgatc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga acatcaaggt
    64681 ttgctgttgg gaagtgtcac aacagcagtt ccactttcag acgaacaacg aatacaatta
    64741 gaaaagaatg ttgcaaaaac aatgaactat caatcagtcg aattaaaaca aatcgttgat
    64801 tcttctatta ttggtggcgc gattgttgaa gctaaccatc gagtaatcga cggaagcatt
    64861 cgcacgcaat tagaaaatat gcgtaatcaa ttaaatagat agtacaaaga ggtaggtgaa
    64921 tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa ttgaaaatta
    64981 tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg acgggatcgc
    65041 tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt caaatggctc
    65101 atacggaatg gcgcaaaact tagaatcaaa tgatgtaggt atcattatcc ttggcgattt
    65161 cgagtccatt cgtgaaggag ataaagtaaa acgaacagga aagatcatgg aagttccagt
    65221 tggagaggcc ttgattggac gggtagttaa tccgctaggt cagccaattg acggactagg
    65281 tgaaatcgtt actgacaaag ctcgtccagt agaagcaatg gcaccaggcg ttatgcaacg
    65341 taaatctgtt aacgaaccaa tgcaaacagg tctaaaagca atcgacgctc ttgtaccaat
    65401 cggacgcggc caacgtgaat tagttatcgg tgaccgtaaa acagggaaaa cttccattgc
    65461 gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg tagcaatcgg
    65521 acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg gcgcaatgga
    65581 ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagcg ccgttgcttt atatcgcacc
    65641 atatgctggt actgcaatgg gtgaagagtt catgtacaac gggaaacatg tcttgatcat
    65701 ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc tgcttcgccg
    65761 tccaccaggt cgtgaagctt atccagggga tgttttctac ttacactcac gcttattgga
    65821 acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct taccatttgt
    65881 tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt cgatcactga
    65941 tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag cggttgatgc
    66001 cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga aaaaagttgc
    66061 tgggacttta cgtctagact tagcaagtta ccgtgaatta gaagcattta cacaattcgg
    66121 ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta cagtagaaat
    66181 cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga ttttgtatgc
    66241 tttgacacat ggattcttag atagtatccc tgtagacagt attttagact tcgaacatga
    66301 attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc gtacaacaaa
    66361 agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag acatgttctt
    66421 agctacaaaa ggaaataatt cctctacaga agataaattg aaatccattc aaaatgcata
    66481 gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc gcttcaacga
    66541 aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag ttgacaaaat
    66601 ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa atcgttacgc
    66661 atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg gatatcaatt
    66721 acaacagcat gttgctcagc cgtcctgtta agaaaacggg ttatatcgtc atcactgcag
    66781 atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg tctattttag
    66841 aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga actggtgcag
    66901 acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta tctgatcagc
    66961 caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgtac caaaacgaag
    67021 tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact agtcagtttc
    67081 gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaact actttcgaac
    67141 aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg cctcagtatg
    67201 ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat gctgctggta
    67261 tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag ctgacggttt
    67321 cttacaaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt gttgccggag
    67381 cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg caagattgtt
    67441 caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt accagatatc
    67501 aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt tcttgaagca
    67561 gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac agatggttta
    67621 caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt aggtaaagaa
    67681 acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac acctttccca
    67741 gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga attaagtaca
    67801 agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc atatttaaaa
    67861 ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact gatccaagaa
    67921 ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg tgtaggtgaa
    67981 cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt tatcgaaaaa
    68041 acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg tgttgctttg
    68101 actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt actgttgttt
    68161 atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt gttaggacgg
    68221 atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca attgcaagaa
    68281 cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta tgtgccagcc
    68341 gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc gacaaccaac
    68401 ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt ggcttcttct
    68461 tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc aacagaagtc
    68521 caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt aggtatggat
    68581 gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca attcttcttg
    68641 tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata tgtccctgta
    68701 gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga tcttcctgaa
    68761 gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa aacactaggc
    68821 tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa ctcctgccgg
    68881 tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg gtgagcttgg
    68941 gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag ttcgtgtaaa
    69001 acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa tcatggaagt
    69061 tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg atatcgatgt
    69121 atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag caaaagcaaa
    69181 acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa tcaatcgtat
    69241 caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt aagaacagta
    69301 aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt acgaaaattg
    69361 cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc tggaacaccg
    69421 tatatcggag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aatggaaaat
    69481 agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat tgaacaccgt
    69541 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
    69601 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
    69661 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
    69721 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
    69781 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
    69841 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
    69901 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
    69961 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
    70021 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
    70081 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
    70141 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
    70201 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
    70261 tgaacaaaag aatcaagtaa caatcgatgc ttcaagacaa ttagaaatcg aagcgcctta
    70321 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
    70381 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
    70441 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
    70501 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
    70561 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
    70621 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
    70681 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
    70741 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
    70801 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
    70861 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
    70921 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
    70981 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
    71041 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
    71101 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
    71161 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
    71221 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
    71281 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
    71341 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
    71401 tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
    71461 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc caagtttgtg
    71521 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
    71581 gaagtgagcc atgaagcaga acatgacaaa agaaaacaga agggaacgtt gacgttagtc
    71641 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
    71701 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
    71761 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc actaaaaggg
    71821 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
    71881 tatgtaccat tgacattcca attaacgcga aataagaaag ataaaaaaga accagattat
    71941 aaaaaggttg tgaaaaatac gagtagcact gagcgataaa gaaacaattt ggaaaatagc
    72001 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
    72061 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg aaaattattg
    72121 tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga agaactaaac
    72181 aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga aaaagttcaa
    72241 gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac aataggtgta
    72301 ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt agccccaacg
    72361 tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa gctttcacaa
    72421 ctcgcttcca gtcaaactca gatcatcata cgaaccggca gtactagcag tgcggcgtta
    72481 catctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta ttttatccat
    72541 cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt aattttagca
    72601 attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa atgctttcaa
    72661 atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat ttttatggga
    72721 atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga tggcaaagag
    72781 attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa agcgcaatta
    72841 tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg aatcagctct
    72901 cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat tccttatatt
    72961 catggattta atattttccg agatttggaa actctgtttc aactcccagt aacgattgaa
    73021 aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg tgattatcaa
    73081 gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac gaaaggacaa
    73141 atcaataaag gggcacatct ttatggtgga gaatttgggt tgatgttctt aaatggcgat
    73201 aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg tgaacgcaaa
    73261 ggtctcgata aaaattctta taccggaaaa gatgtctttg aattagcaga aactggagat
    73321 gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg attattcagt
    73381 attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggtgtttc tgcaaaagaa
    73441 gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt cgatctgcat
    73501 gatttcgaac cgcagatttt attatgtgaa tacagaaacg atgctaatct tgtaggtgca
    73561 gcagcaaact atctcgctgt acgtcaaggg gagaattaag agctttagag aaggtgcagc
    73621 ttgcataaaa gaagtgaatg aaatgaatag gagattttta cctaaggaat gttttatttg
    73681 ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaaa taaagatgtt
    73741 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
    73801 ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa agaatatgaa
    73861 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
    73921 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
    73981 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
    74041 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
    74101 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
    74161 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
    74221 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
    74281 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
    74341 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
    74401 gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc caacgaatca
    74461 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
    74521 ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa aaattgtttc
    74581 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
    74641 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
    74701 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
    74761 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
    74821 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
    74881 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcgcgagaaa aagtacaaga
    74941 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcgcaaaatc gagtaccgtc
    75001 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
    75061 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
    75121 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
    75181 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
    75241 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
    75301 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
    75361 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
    75421 aacaggtgat ccctgattcc ttacttagaa ataaaagaga atcagaatca cctgttttta
    75481 tatatgatca gttaccagtt gctcgtgata agaaacagga ccctcttcaa attggacttg
    75541 tccgttcagc agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc
    75601 acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc
    75661 aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg
    75721 caaggtacct tctacgattc caatgtcaga caatgcttga gagacagcat gagtgtatgc
    75781 tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc
    75841 tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgccag ccgttccact
    75901 tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct
    75961 gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttg
    76021 gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga tctcaatctc
    76081 actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttt
    76141 cattttcttt attcagtata aataaagttc taggttgttt caacactgta acggttgcgc
    76201 aatcgctgta gtatgcgata ataaagacaa aggtaggtgg atctggggat gcaggaacga
    76261 atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg
    76321 ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca
    76381 agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc
    76441 tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc
    76501 aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg
    76561 acaaatttct ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcgtagtt
    76621 tgttttaaaa aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg
    76681 gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg
    76741 actcggatat tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagatg
    76801 tagatgtact gttattagga cctcaagttc gctttatgaa agctgatttt gaaaaacgtc
    76861 tggaaccaaa aggcattcct ttagacgtca tcaatatggc tgattatggt atgatgaatg
    76921 gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact
    76981 atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa
    77041 taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag caatagactg
    77101 tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgggcaactg
    77161 gattctttgt catcgctgtg gaatgaggac gtccgtcatt gatggacagc ttgcagatta
    77221 cggttatttt tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata
    77281 tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct
    77341 tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa aaagacatca
    77401 tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga
    77461 aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga
    77521 attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa
    77581 tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg
    77641 tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg
    77701 ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta
    77761 cttaagtgcc acacctgata aaaagttact aaaggaaatc aagcagacgt ttgacatcca
    77821 caaacttgct ttgcgctttc atcggcgttt acttcctgaa cccgtcttgg ttttcatgaa
    77881 taattggcga caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca
    77941 tcaattaatt aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg
    78001 tttgaaagcc acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag
    78061 agacaatcag cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt
    78121 aaccacgatg atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg
    78181 agcggatcat ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg
    78241 taaagggcct tatacgaaca gtcaagtata ctttttctat gaagaaaaaa ctgcagctat
    78301 tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tgctgtgagg
    78361 tgcagttgct gcagtaaaga tatcgtccaa aatttaacat tcagcgagat tattggtgtt
    78421 tcgaaataca gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa
    78481 acaagaggct gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag
    78541 aaagtaaagt ctattcccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg
    78601 acctatgatt ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc
    78661 aatcaataca agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg
    78721 cagataaaaa aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg
    78781 cgaacagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt
    78841 cagatgctac tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa
    78901 agattggaga tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa
    78961 aaaatattga ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat
    79021 tgtttgcagg tcgcacagcc gaaaatcatc cgaaccattt ctttagcaag atgaattaga
    79081 tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta
    79141 agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt
    79201 tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta
    79261 cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc
    79321 gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt
    79381 accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt
    79441 ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc
    79501 ccgtgaaacg ggcatgaata ctgcagatgc tactgcttta ttcaatgaag aggacgaaaa
    79561 cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga
    79621 tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta tttttgaaga
    79681 tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat
    79741 tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag ctactgacag
    79801 ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata aattatgaag
    79861 ttcttacttt aatacgctta agatatttca attatctata actaatgata aaatgaaaag
    79921 taaagtctac atagtctgga taatacacag aaggaaaagg agattacaca acgcatggcc
    79981 aatttgattc gttcacttat cgaaaacgat aagaaagaac taaaacgttt agatagcatt
    80041 gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat taactgatga acaattacag
    80101 gcaaaaactg ctgaattcaa gggcagatac caaaaaggag aaacattaga tcaattactt
    80161 ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg tattggggtt atatccacac
    80221 cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa atatccctga aatgcgtact
    80281 ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc taaatgctct gactggcgaa
    80341 ggggtacacg tagttaccgt caatgaatat ttagcaaccc gtgactcgac ggaaatgggc
    80401 gaactttata atttcttagg attaacagtg gggttgaata tcaactcaaa atcttcggat
    80461 gaaaaacgcg aggcttataa ttgtgatatc acttatagta cgaataatga attaggattt
    80521 gactacttga gagacaatat ggtcgtttac cgccatcaaa tggtacaacg tccgctgaat
    80581 tatgccattg tcgatgaagt tgactcaatc ttgattgatg aagcgagaac gccgctgatc
    80641 atttcagggc aagcagaaaa atccacagct ttatatacaa gagcagacaa cttcgtgaaa
    80701 cgtttgaaag aagaagaaga ttataaaatc gatgtacaat caaaaacaat cagtttaacc
    80761 gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg ataacttata cgacattgaa
    80821 aatactgcat tgacccatca cttggatcaa gcattgagag ccaactatat catgctttta
    80881 gatatcgatt atgttgtcca agacggaaaa gtactgattg ttgaccagtt cactggtcgg
    80941 atcatggatg gacgccgtta ttcagatgga cttcaccaag caatcgaagc taaagaaggc
    81001 gtagagatcg aagacgaaac gaaaaccatg gcgacgatca ctttccagaa ctatttccgt
    81061 atgtacaaga aacttgcggg aatgactggt acagctaaaa cagaagaaga agaattcaga
    81121 gaaatttata acatccaagt ataccagatt ccgacaaatc gtccggttat ccgtgatgat
    81181 cgtccagatt tgctttaccc aacattacaa agcaagttcc acgctgtcgt gcaagatatc
    81241 aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa ctgttgcggt tgaaacatct
    81301 gagttattat cagatatgct gaatcaagaa cgtattcctc atgaagtgct gaatgcgaaa
    81361 aatcacttta aagaagcaga gatcatcatg aatgctggtc aaaaaggtgc tgtgacgatt
    81421 gctaccaaca tggctggtcg gggaacagat atcaagttag gacttggggt aagagaactt
    81481 ggtggtctag cagttatcgg gacagaacga catgaatctc gtcgtatcga taaccagctt
    81541 cgaggacgtg caggacgtca aggagaccct ggtatgtcac agttctactt gtcattggaa
    81601 gatgatttga tgaaacgatt cggttcagaa cgaatcaaat ctttcttaga tcggatgaag
    81661 atcggtgagg aagatgctgt tatccaaagc aagatgctga cgaaacaagt agaatctgca
    81721 caaaaacgtg tagaaggaaa taactatgac acacgtaaaa acgtcttaca atacgatgat
    81781 gtgatgcgtg aacaacgtga agtaatctat aaacagcgac aagaagtaat catggaagaa
    81841 aaaagcttat ccaagccact aatgaacatg gtcaaacgga caatcagtcg tgtagtagat
    81901 gcgcatacac aattagagaa agaaaattgg aatttggaaa gtatcatcga ttttgccggc
    81961 agtactcttg tccatgaaga tagtattact cttgcagata tcgaaggaaa aacagctcag
    82021 gagatcaaag agtatctaaa cgaacgagca caagaagtat ttaataccaa agcggctcaa
    82081 ctaaatggtc cagagcaact gttggaattt gaaaaagtcg tgatcttgcg tgttgtggac
    82141 tctaaatgga cagaccatat tgatgcaatg gatcaattac gtcagtcaat cggcttacgt
    82201 gcttatggac agaacaatcc attagtcgaa tatcaaacag aaggctataa gatgtttgaa
    82261 gatatggtag gcgccatcga gtatgaagtc actcgaatct ttatgaaagc tgagatccgt
    82321 caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt ctcactcaga agacggagat
    82381 gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa tccatgtgaa caaggtcgga
    82441 agaaatgatc cttgtccttg tggaagcggg aaaaaatata agaactgtca tggaaaaaat
    82501 gcaaactaat ttctgacagc aggattggtg agacaaattc tttttttaag aattcgtctc
    82561 accttctttc tgttatttgc tgattctgtt agactagata taatgaaaaa agtatagtga
    82621 aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct cctgacagaa atgcaggaaa
    82681 aggtcacaag cttcaggagg tctctttgac ttagaccagc tggaagaaga cattgcggaa
    82741 gcagaagcaa aaatggctga acctggtttt tgggatgata gcgaagctgc tcaaaaagtg
    82801 atcagtgaaa acaatgcaaa taaagaaact tatgatcaat tccattctct tgctgaagaa
    82861 ctggaagaaa tcgaattgat ggtggagatg ttgcaggaag aacctgatgc agccatgcaa
    82921 gaagaagcag aaacacgtat caatgcgcta gatgaaaaaa tgaagacata tgaattatct
    82981 atgttgttag atggtcctta tgatcgaaac aacgccatcg ttgaattgca tccaggggct
    83041 ggcggaacgg agtcccaaga ttggggcagt atgcttttac gtatgtatac acgctgggca
    83101 gagcagcatg ggtatcaagt agagacacta gattatcaag caggtgatga agcaggaatc
    83161 aagagtgtga ccctactgat caaaggacac aacgcatttg gttatctgaa atcagaaaaa
    83221 ggtgttcatc gccttgtacg tatctcgcct tttgactcag cgaaacgacg tcatacttct
    83281 ttttgttcgg tagatgtgat gccagagctt gatgaaacga tcgatataga aatcaatcca
    83341 gatgatttga aaatcgatac gtaccgagca agtggtgcag gtggtcagca catcaataaa
    83401 acagaatctg ctgtacggat cactcacttg cctacaggaa cagtagtagc tagtcaggca
    83461 caacgttcac aattgaaaaa ccgtgagcaa gcgatgggga tgttaaaagc taagctttat
    83521 caattagaag tggaaaagaa agaacaagaa gcagctgcac ttcgaggaga acaaaaagaa
    83581 atcggctggg gttcacagat ccgttcttat gttttccatc catattctat ggtaaaagac
    83641 catcggacaa acgtagagac aggaaatgtc caagcagtta tggacggtga ccttgattta
    83701 tttattgacg gctatttaaa aatgcatcta caataagaac ccattgttaa attgaaataa
    83761 aattgtaaga ttttgcaagc atgttgaaac attgccatgc tataatagct aaagactgaa
    83821 aaagatatgg agagaacgcc atgattgaaa tgaaggatgt aatgaagaag tactcaaatg
    83881 gtacgaccgc tatccgcaat ctctcaattg tcatcgaaca aggagagttt gtctatgtgg
    83941 ttggtccgtc tggagccggt aaatctacat tcatcaaatt gatgtatcga gaagaaaaag
    84001 cttcaaaagg aactttgaac gtcgcaggtc atgatctaat agcaatcaaa aaccgcgaag
    84061 ttccttatct acgcagagaa atcggcgtgg tgttccaaga ctataaatta ttaccaagaa
    84121 aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat cggcagaaaa cctcgggata
    84181 tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt gaaacacaaa gttcgagtct
    84241 ttcctagcga attatccggt ggggaacagc aacgtgtctc tattgctcgg gcaatagtga
    84301 atacgcctaa agtacttatc gcggatgaac cgacaggaaa tctggatcca gaaaactcat
    84361 gggaaatcat gaaattatta gatcggatca atgcccaagg aactactgtc gtgatggcta
    84421 cacataacag tacgatcgta aatacgattc ggcaccgagt aattgctatc gaaaacggcc
    84481 gcattattcg agatcaagcg gaaggggaat atggatacga tgattagaac ctttttccgc
    84541 catttattag aaagtatcaa gagtttgaaa cgtaacggct ggatgacagt tgcttcagtc
    84601 agtgcggtaa cgatcacatt gactttagtc ggtgtattca tggcagttat catgaacgca
    84661 actaaattag ctcaagatat agaaggaaac gttgatgtct ctgtattcgt agatatcggt
    84721 acaaaagacg aagagatgaa aaagctagaa aaagaattga atgacttaga tcatgttaaa
    84781 aaagttgaat tttcaagcaa agaacaggaa ttgaaaaaaa tccaaaacga aatgggtgat
    84841 tcctggaaat tgtttgaagg tgacaataac ccgttatatg atgtgtatgt agtaagtgct
    84901 actgatcctt cttatacaaa acaagtagca gaagaagcag cagatttgaa gaatgtttct
    84961 cgttctgatt acggtggagc ttcctcagat cggattttcc aaatcgctgg tgcagttcgg
    85021 acatggggat tagttgcagc ggcattgctg ctgttcgtag cgatgtttct catttcgaac
    85081 acgattcgga tcacgatttt gtctagacag cgtgaaattc aaatcatgcg tttagtcggt
    85141 gcaaaaaatg gatatattcg ctggccgttt ttccttgaag gtggctggat cggtctgatc
    85201 ggtgcgattc ttccgatttt gatcatcaca tttggttatg catggttttt ccaattgatc
    85261 aatccatcat tattacgttc ccattattca ttgattcatc cacaaaatat cgtttggaaa
    85321 atcaatcttt taatggtcgg cattggtgtg atcattggtt cacttggctc gatcatttct
    85381 atgagacgtt tcttgaaatt ctaataccac aatataaata aaatgagaaa gttggacagt
    85441 ttcgttccag ctttcttttt ttattggcta ggagaataga aggaagcggc ttttttccct
    85501 ttactttaaa aaacaacttg ggtattctta agttggtaga agaagaggtg aagccgtgaa
    85561 aattatcgtt gacaattttt ttacgatcct tttgattatg aatattttgc tttcctttat
    85621 catcgttttt cgagaaagaa aggaaactgc tcaaacatgg gcatgggtgc tcgttttgat
    85681 gttcattcca gttgtaggat ttattttata tattttttta ggaagaggga tttccaaaga
    85741 gaaaatcttt gatttgaaga tccaagggaa aatcggtaag aacctggaaa ttgaagaaga
    85801 caaacaggca ttgatgcgag ggctttatcc tcatccgcct actggacaag tagatgtgaa
    85861 acaattgatc tatatgctca cggtatttga aagtacgctc tatacgacta aaaatgaaat
    85921 cactttattt acagatggtc gtgaaaaatt cgatgcgctg attgaagata tcaagcaggc
    85981 agaagaccat atccacttcc agtattatat ttatcgcagt gatgcattag gtgaagaagt
    86041 tcgagatgca ttgacagagg cggcaagacg aggggtaaaa gtcagagtcc ttcttgatgc
    86101 atggggttcc acacaagttt cttccagctt ttttcagaat ttgaaaaaag caggcggaga
    86161 agtcgcattt ttctttcctc tatttgttcc gtatattaat cctcggatca attatcgcaa
    86221 tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat acaggtgggt tcaatgttgg
    86281 aaatgaatat ctaggattgg taaagaaatt tggttattgg cgagacaatc atttgcgtat
    86341 ttacggagaa gcggtgtata ccttgcaaaa ccgttttttg atggactgga attctcagca
    86401 tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc attcactcta ctggtgatat
    86461 agctgttcaa atcgtgacaa gcggaccaga taccgaacat gaacaaatca aaatgactta
    86521 tttgaaaatg atttcgttag ccaaacggga gatattgatc caaacgcctt attacatacc
    86581 ggatggatcg attcatgaag cactgaagtt ggctcttctt tcaggcgtaa aagttcatat
    86641 ccaaatacct aacaaaccag atcatcttct cgtttactgg gcaacgtatt ctttcgctgc
    86701 agagctaatc gagtatggag caaggatcga aacatatgag aatggattta ttcatgcaaa
    86761 gacgatgatt atcgatggtg gcattgtttc agttggctca gcaaatatcg atgttcgttc
    86821 atttcgcttg gactttgagg tcaatacact ggtttatgat gaacgaatgg cttcacgtgt
    86881 aagaaaagct ttttttgaag actcgaaaat ttctacgcat ctcacaaaag aaatgtatga
    86941 aaaccgagga ataatcatca agatgaaaga aggattagca agattgattt cgccgctcct
    87001 ttaaaacata aaaaggacat gagaaccatc aacagatgga gatctcgtgt ccttttctaa
    87061 cggggccatt tctgagagta cgtcagaaat ggagaaagcg tctcgcatga gcgaaattaa
    87121 ctttggggta tctcttgttt tttggggaaa gcaagaaata agctaatata tgctattaaa
    87181 agagacacat ttcaaagcca gtatagcata gaaactttta atgagatagt tttttttaca
    87241 attatgagaa tttattttgt tatttaatga ttttgattcg ttttcatcaa gtaatgaaaa
    87301 tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt
    87361 cttaccaata ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg
    87421 agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga
    87481 aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac
    87541 tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga
    87601 agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat
    87661 cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa
    87721 actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt
    87781 tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga
    87841 cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt
    87901 tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga
    87961 tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac
    88021 gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt
    88081 tttagactat atcttatctg atgaagtaca agataacatc gttggagaac taggttatat
    88141 ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc
    88201 tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt
    88261 tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga
    88321 tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt
    88381 catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt
    88441 agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt
    88501 tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt
    88561 gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt
    88621 tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt
    88681 acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt
    88741 atcagttatc gtgccttttg tcagaagtat ttttggcggg actgggtttg gtatcttagc
    88801 tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc
    88861 attaaaggca gtgccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg
    88921 gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt
    88981 ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc
    89041 agcattgatg ccaacaggtc ttactactcc tgcttctaca ttgacgtcca tcttaacaat
    89101 gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct
    89161 gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg
    89221 agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata
    89281 gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga
    89341 ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggcggt
    89401 ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt
    89461 tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta
    89521 acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt
    89581 ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt
    89641 gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta
    89701 caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa
    89761 acagtgataa aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta
    89821 agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca
    89881 cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc
    89941 cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct
    90001 gatggtgttt ctgtttcggc tggcgcttct gccgtgttga ttattgctgt tttactgttc
    90061 aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga
    90121 gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag
    90181 agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac aatgaagcaa
    90241 tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg
    90301 gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata
    90361 cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg
    90421 tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt
    90481 cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat
    90541 tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca
    90601 gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgtattgctc
    90661 gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc
    90721 ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca
    90781 tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct
    90841 atttaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc
    90901 aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa
    90961 aagagattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag
    91021 ggatcgatct aagcttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg
    91081 gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga
    91141 tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg
    91201 aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct
    91261 atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc
    91321 aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc
    91381 ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt
    91441 tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt
    91501 ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg
    91561 tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg
    91621 gtcacttgat cgaatacaat gatacgaaga agatattcat gaatccagaa aaacaagaga
    91681 cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat
    91741 ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg atggtgagca
    91801 gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag
    91861 ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg
    91921 aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga
    91981 aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga
    92041 ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg
    92101 attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag
    92161 acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata
    92221 atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact
    92281 tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga
    92341 tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct
    92401 aaactgaagc tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt
    92461 gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaaatagc tcctcatatt
    92521 tttcgtttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgggaa
    92581 agggtttgtg acaaaacatg tcacaaaccc tt
//
LOCUS       DAFFFQ010000003        78935 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000003
VERSION     DAFFFQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.003"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            374..3181
                     /locus_tag="LQB69_000232"
     CDS             374..3181
                     /locus_tag="LQB69_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3524424.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            3372..3863
                     /locus_tag="LQB69_000233"
     CDS             3372..3863
                     /locus_tag="LQB69_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3524425.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3966..4955
                     /gene="manX"
                     /locus_tag="LQB69_000234"
     CDS             3966..4955
                     /gene="manX"
                     /locus_tag="LQB69_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL3524426.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4988..5791
                     /locus_tag="LQB69_000235"
     CDS             4988..5791
                     /locus_tag="LQB69_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL3524427.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5812..6726
                     /locus_tag="LQB69_000236"
     CDS             5812..6726
                     /locus_tag="LQB69_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL3524428.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6809..7183
                     /locus_tag="LQB69_000237"
     CDS             6809..7183
                     /locus_tag="LQB69_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL3524429.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7322..7567
                     /locus_tag="LQB69_000238"
     CDS             7322..7567
                     /locus_tag="LQB69_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3524430.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7739..8803
                     /locus_tag="LQB69_000239"
     CDS             7739..8803
                     /locus_tag="LQB69_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL3524431.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9049..9474
                     /locus_tag="LQB69_000240"
     CDS             9049..9474
                     /locus_tag="LQB69_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3524432.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9467..9916
                     /gene="nusB"
                     /locus_tag="LQB69_000241"
     CDS             9467..9916
                     /gene="nusB"
                     /locus_tag="LQB69_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL3524433.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            10013..10867
                     /locus_tag="LQB69_000242"
     CDS             10013..10867
                     /locus_tag="LQB69_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL3524434.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10868..12205
                     /locus_tag="LQB69_000243"
     CDS             10868..12205
                     /locus_tag="LQB69_000243"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL3524435.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12210..12434
                     /locus_tag="LQB69_000244"
     CDS             12210..12434
                     /locus_tag="LQB69_000244"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL3524436.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12436..13326
                     /locus_tag="LQB69_000245"
     CDS             12436..13326
                     /locus_tag="LQB69_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL3524437.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13335..14153
                     /locus_tag="LQB69_000246"
     CDS             13335..14153
                     /locus_tag="LQB69_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL3524438.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14260..14709
                     /locus_tag="LQB69_000247"
     CDS             14260..14709
                     /locus_tag="LQB69_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL3524439.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14727..16409
                     /gene="recN"
                     /locus_tag="LQB69_000248"
     CDS             14727..16409
                     /gene="recN"
                     /locus_tag="LQB69_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL3524440.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16464..16580)
                     /locus_tag="LQB69_000249"
     CDS             complement(16464..16580)
                     /locus_tag="LQB69_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL3524441.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16653..17609)
                     /locus_tag="LQB69_000250"
     CDS             complement(16653..17609)
                     /locus_tag="LQB69_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3524442.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17780..18148
                     /locus_tag="LQB69_000251"
     CDS             17780..18148
                     /locus_tag="LQB69_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL3524443.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18319..18750
                     /gene="mraZ"
                     /locus_tag="LQB69_000252"
     CDS             18319..18750
                     /gene="mraZ"
                     /locus_tag="LQB69_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL3524444.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18771..19730
                     /gene="rsmH"
                     /locus_tag="LQB69_000253"
     CDS             18771..19730
                     /gene="rsmH"
                     /locus_tag="LQB69_000253"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL3524445.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19735..20136
                     /gene="ftsL"
                     /locus_tag="LQB69_000254"
     CDS             19735..20136
                     /gene="ftsL"
                     /locus_tag="LQB69_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL3524446.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20137..22329
                     /locus_tag="LQB69_000255"
     CDS             20137..22329
                     /locus_tag="LQB69_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL3524447.1"
                     /translation="MSLKNKIRRFMEKKNLNPINNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22355..23317
                     /locus_tag="LQB69_000256"
     CDS             22355..23317
                     /locus_tag="LQB69_000256"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL3524448.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23331..24692
                     /locus_tag="LQB69_000257"
     CDS             23331..24692
                     /locus_tag="LQB69_000257"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL3524449.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24718..25806
                     /gene="murG"
                     /locus_tag="LQB69_000258"
     CDS             24718..25806
                     /gene="murG"
                     /locus_tag="LQB69_000258"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL3524450.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25821..27041
                     /locus_tag="LQB69_000259"
     CDS             25821..27041
                     /locus_tag="LQB69_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL3524451.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27197..28522
                     /gene="ftsA"
                     /locus_tag="LQB69_000260"
     CDS             27197..28522
                     /gene="ftsA"
                     /locus_tag="LQB69_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL3524452.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28545..29786
                     /gene="ftsZ"
                     /locus_tag="LQB69_000261"
     CDS             28545..29786
                     /gene="ftsZ"
                     /locus_tag="LQB69_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL3524453.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29803..30480
                     /locus_tag="LQB69_000262"
     CDS             29803..30480
                     /locus_tag="LQB69_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL3524454.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30494..31093
                     /locus_tag="LQB69_000263"
     CDS             30494..31093
                     /locus_tag="LQB69_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL3524455.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31108..31395
                     /locus_tag="LQB69_000264"
     CDS             31108..31395
                     /locus_tag="LQB69_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL3524456.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31444..32226
                     /locus_tag="LQB69_000265"
     CDS             31444..32226
                     /locus_tag="LQB69_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL3524457.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32247..32954
                     /locus_tag="LQB69_000266"
     CDS             32247..32954
                     /locus_tag="LQB69_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL3524458.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33216..36014
                     /gene="ileS"
                     /locus_tag="LQB69_000267"
     CDS             33216..36014
                     /gene="ileS"
                     /locus_tag="LQB69_000267"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL3524459.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36063..37589)
                     /locus_tag="LQB69_000268"
     CDS             complement(36063..37589)
                     /locus_tag="LQB69_000268"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL3524460.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37604..38251)
                     /locus_tag="LQB69_000269"
     CDS             complement(37604..38251)
                     /locus_tag="LQB69_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL3524461.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38435..38764)
                     /locus_tag="LQB69_000270"
     CDS             complement(38435..38764)
                     /locus_tag="LQB69_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL3524462.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38941..39669
                     /gene="sfsA"
                     /locus_tag="LQB69_000271"
     CDS             38941..39669
                     /gene="sfsA"
                     /locus_tag="LQB69_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL3524463.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39685..40698
                     /locus_tag="LQB69_000272"
     CDS             39685..40698
                     /locus_tag="LQB69_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3524464.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40698..41975
                     /locus_tag="LQB69_000273"
     CDS             40698..41975
                     /locus_tag="LQB69_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3524465.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            42038..44740
                     /locus_tag="LQB69_000274"
     CDS             42038..44740
                     /locus_tag="LQB69_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3524466.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44892..45209
                     /locus_tag="LQB69_000275"
     CDS             44892..45209
                     /locus_tag="LQB69_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3524467.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45239..45559
                     /locus_tag="LQB69_000276"
     CDS             45239..45559
                     /locus_tag="LQB69_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3524468.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45667..47127
                     /locus_tag="LQB69_000277"
     CDS             45667..47127
                     /locus_tag="LQB69_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3524469.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47195..47416
                     /locus_tag="LQB69_000278"
     CDS             47195..47416
                     /locus_tag="LQB69_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524470.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47447..47629
                     /locus_tag="LQB69_000279"
     CDS             47447..47629
                     /locus_tag="LQB69_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL3524471.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47629..48042
                     /locus_tag="LQB69_000280"
     CDS             47629..48042
                     /locus_tag="LQB69_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL3524472.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48165..49346
                     /locus_tag="LQB69_000281"
     CDS             48165..49346
                     /locus_tag="LQB69_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524473.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(49417..49581)
                     /locus_tag="LQB69_000282"
     CDS             complement(49417..49581)
                     /locus_tag="LQB69_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524474.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            49735..49808
                     /locus_tag="LQB69_000283"
     tRNA            49735..49808
                     /locus_tag="LQB69_000283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49769..49771,aa:Arg,seq:cct)
     gene            complement(49877..51016)
                     /locus_tag="LQB69_000284"
     CDS             complement(49877..51016)
                     /locus_tag="LQB69_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3524475.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51315..51950)
                     /locus_tag="LQB69_000285"
     CDS             complement(51315..51950)
                     /locus_tag="LQB69_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524476.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52063..52698)
                     /locus_tag="LQB69_000286"
     CDS             complement(52063..52698)
                     /locus_tag="LQB69_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL3524477.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52732..53193)
                     /locus_tag="LQB69_000287"
     CDS             complement(52732..53193)
                     /locus_tag="LQB69_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL3524478.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53323..53754)
                     /locus_tag="LQB69_000288"
     CDS             complement(53323..53754)
                     /locus_tag="LQB69_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL3524479.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53772..54092)
                     /locus_tag="LQB69_000289"
     CDS             complement(53772..54092)
                     /locus_tag="LQB69_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3524480.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54391..55167
                     /locus_tag="LQB69_000290"
     CDS             54391..55167
                     /locus_tag="LQB69_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL3524481.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55182..55385
                     /locus_tag="LQB69_000291"
     CDS             55182..55385
                     /locus_tag="LQB69_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524482.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55401..55739
                     /locus_tag="LQB69_000292"
     CDS             55401..55739
                     /locus_tag="LQB69_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL3524483.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55726..55905
                     /locus_tag="LQB69_000293"
     CDS             55726..55905
                     /locus_tag="LQB69_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524484.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(55948..56418)
                     /locus_tag="LQB69_000294"
     CDS             complement(55948..56418)
                     /locus_tag="LQB69_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524485.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56505..57203
                     /locus_tag="LQB69_000295"
     CDS             56505..57203
                     /locus_tag="LQB69_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL3524486.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57381..57722
                     /locus_tag="LQB69_000296"
     CDS             57381..57722
                     /locus_tag="LQB69_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524487.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57715..58386
                     /locus_tag="LQB69_000297"
     CDS             57715..58386
                     /locus_tag="LQB69_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL3524488.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58392..59078
                     /locus_tag="LQB69_000298"
     CDS             58392..59078
                     /locus_tag="LQB69_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524489.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            59081..59830
                     /locus_tag="LQB69_000299"
     CDS             59081..59830
                     /locus_tag="LQB69_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524490.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59842..60111
                     /locus_tag="LQB69_000300"
     CDS             59842..60111
                     /locus_tag="LQB69_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524491.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            60117..60281
                     /locus_tag="LQB69_000301"
     CDS             60117..60281
                     /locus_tag="LQB69_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524492.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            60274..60573
                     /locus_tag="LQB69_000302"
     CDS             60274..60573
                     /locus_tag="LQB69_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524493.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            60573..60875
                     /locus_tag="LQB69_000303"
     CDS             60573..60875
                     /locus_tag="LQB69_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL3524494.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            60915..61280
                     /locus_tag="LQB69_000304"
     CDS             60915..61280
                     /locus_tag="LQB69_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524495.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            61770..61910
                     /locus_tag="LQB69_000305"
     CDS             61770..61910
                     /locus_tag="LQB69_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524496.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            61907..62095
                     /locus_tag="LQB69_000306"
     CDS             61907..62095
                     /locus_tag="LQB69_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524497.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            62103..62297
                     /locus_tag="LQB69_000307"
     CDS             62103..62297
                     /locus_tag="LQB69_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524498.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(62330..62542)
                     /locus_tag="LQB69_000308"
     CDS             complement(62330..62542)
                     /locus_tag="LQB69_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524499.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            62667..62870
                     /locus_tag="LQB69_000309"
     CDS             62667..62870
                     /locus_tag="LQB69_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524500.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            62867..63139
                     /locus_tag="LQB69_000310"
     CDS             62867..63139
                     /locus_tag="LQB69_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL3524501.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            63140..63325
                     /locus_tag="LQB69_000311"
     CDS             63140..63325
                     /locus_tag="LQB69_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524502.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            63312..63659
                     /locus_tag="LQB69_000312"
     CDS             63312..63659
                     /locus_tag="LQB69_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524503.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            63619..64095
                     /locus_tag="LQB69_000313"
     CDS             63619..64095
                     /locus_tag="LQB69_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL3524504.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(64241..64969)
                     /locus_tag="LQB69_000314"
     CDS             complement(64241..64969)
                     /locus_tag="LQB69_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL3524505.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            65017..65295
                     /locus_tag="LQB69_000315"
     CDS             65017..65295
                     /locus_tag="LQB69_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524506.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            65297..65683
                     /locus_tag="LQB69_000316"
     CDS             65297..65683
                     /locus_tag="LQB69_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524507.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            65680..66060
                     /locus_tag="LQB69_000317"
     CDS             65680..66060
                     /locus_tag="LQB69_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL3524508.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            66172..66624
                     /locus_tag="LQB69_000318"
     CDS             66172..66624
                     /locus_tag="LQB69_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL3524509.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            66621..68348
                     /locus_tag="LQB69_000319"
     CDS             66621..68348
                     /locus_tag="LQB69_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL3524510.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            68412..69599
                     /locus_tag="LQB69_000320"
     CDS             68412..69599
                     /locus_tag="LQB69_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL3524511.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            69571..70140
                     /locus_tag="LQB69_000321"
     CDS             69571..70140
                     /locus_tag="LQB69_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL3524512.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            70153..71517
                     /locus_tag="LQB69_000322"
     CDS             70153..71517
                     /locus_tag="LQB69_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL3524513.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            71519..71800
                     /locus_tag="LQB69_000323"
     CDS             71519..71800
                     /locus_tag="LQB69_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL3524514.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            71778..72104
                     /locus_tag="LQB69_000324"
     CDS             71778..72104
                     /locus_tag="LQB69_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL3524515.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            72094..72432
                     /locus_tag="LQB69_000325"
     CDS             72094..72432
                     /locus_tag="LQB69_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524516.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            72422..72787
                     /locus_tag="LQB69_000326"
     CDS             72422..72787
                     /locus_tag="LQB69_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL3524517.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            72794..73420
                     /locus_tag="LQB69_000327"
     CDS             72794..73420
                     /locus_tag="LQB69_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3524518.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            73420..73884
                     /locus_tag="LQB69_000328"
     CDS             73420..73884
                     /locus_tag="LQB69_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524519.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            74079..76388
                     /locus_tag="LQB69_000329"
     CDS             74079..76388
                     /locus_tag="LQB69_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL3524520.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            76400..77104
                     /locus_tag="LQB69_000330"
     CDS             76400..77104
                     /locus_tag="LQB69_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3524521.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            77101..>78935
                     /locus_tag="LQB69_000331"
     CDS             77101..>78935
                     /locus_tag="LQB69_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3524522.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        26753 a        12984 c        16567 g        22631 t
ORIGIN      
        1 aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac ttaatatttt
       61 tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat ggaagctata
      121 ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt gtttccatta
      181 taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct atccgcgtgt
      241 tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac atagtattat
      301 gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg caaaacgcat
      361 tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga
      421 tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa caaatagcag
      481 taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg gacgaccagt
      541 acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac atgtagtcag
      601 ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata ctaaggatat
      661 ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac aggcaaaagc
      721 tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta ccggatcagg
      781 aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg taatagatga
      841 ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg agcttttgat
      901 gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg
      961 gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc ggttgccgcc
     1021 tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc gtcttgggga
     1081 aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag aagacccagg
     1141 ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac ttccggcttt
     1201 tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg cccacgaagc
     1261 aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat tgatcggcag
     1321 tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat gtgcacgagg
     1381 atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt tgacagaagg
     1441 gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag agctttctaa
     1501 aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga
     1561 ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag ctttactgaa
     1621 gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata tcaacatcca
     1681 tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca aattagcaga
     1741 atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg tcaaaagatc
     1801 tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata tcagttcttt
     1861 tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc gtgagatggc
     1921 ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata tcgaaacgta
     1981 ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac tcgtctcgtt
     2041 acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac
     2101 agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg tacgagcagt
     2161 agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac gcaatgtaca
     2221 agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc ttgcgtcgtt
     2281 taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata tggtaacaac
     2341 ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc tagatatgtt
     2401 gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag aatctgttca
     2461 ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat ctggcgaagg
     2521 tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc aagaaacaga
     2581 attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag ttatccaaaa
     2641 agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag ccccatttat
     2701 tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt tgctgatgga
     2761 atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta aaaatacgtg
     2821 tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa tcgatccaat
     2881 gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag aatatggatt
     2941 caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc ggaatgaacc
     3001 attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt atagccaatt
     3061 ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga tagaagaaat
     3121 gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca ctgtgccatg
     3181 aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt taagaatcct
     3241 ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg
     3301 ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt gaggaagaag
     3361 gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc cacgggcaag
     3421 tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc agcgatgtag
     3481 tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg gggatcacag
     3541 caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta ttatcacgag
     3601 caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc cgctgcggtg
     3661 tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa tcaatgatta
     3721 ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg aacagtcaag
     3781 aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg atggaactac
     3841 tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa taattctgtt
     3901 gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa ataggaggta
     3961 taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga atcttacaat
     4021 ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg
     4081 aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt gataatcaag
     4141 acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa tcaaacacat
     4201 tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga
     4261 ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca gcacacatca
     4321 ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg gcagaagcac
     4381 cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga actgtggtcg
     4441 gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta catggacaag
     4501 ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag
     4561 tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag
     4621 caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt tttggaaata
     4681 ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg
     4741 tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa
     4801 gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag
     4861 gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg gatgatattt
     4921 tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa tttataggag
     4981 gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc tagctggtat
     5041 ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta cattgatcgg
     5101 tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc agatgatcgc
     5161 tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag catcagtagc
     5221 atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc
     5281 gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc
     5341 tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga
     5401 aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc cagcagcagc
     5461 gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc cagcatggtt
     5521 aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg ctttggtaat
     5581 caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg ttgtagctgc
     5641 aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag ctcttattta
     5701 cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca actctggtga
     5761 ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa aatggcagaa
     5821 gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc taccttcatt
     5881 caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc tatgattcca
     5941 gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa acgtcacttg
     6001 gaattcttta atacacaccc atacattgct tctccaattt taggggttac attggctctg
     6061 gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta
     6121 ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac agtacgtcca
     6181 atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg accaatcatc
     6241 ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca agaatttggc
     6301 tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca agacgtaaca
     6361 aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag atgggtatca
     6421 atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta catcgaatgg
     6481 gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt taataatggc
     6541 ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca attgattcct
     6601 ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa aaaggtttct
     6661 ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt catcggactt
     6721 ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata tagtatactg
     6781 ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa aagtagactt
     6841 ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca tgataggtga
     6901 taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa ttccatggga
     6961 agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc ctcgatatgc
     7021 cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta aaaaagtgct
     7081 tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga gtttctttga
     7141 tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa agaaaacttc
     7201 taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat agcaaaacca
     7261 attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag gaggttatat
     7321 tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg caggtgcgat
     7381 caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac tagtcggttc
     7441 aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta tggcacttat
     7501 tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct ttggaaaacg
     7561 atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg tgactccttt
     7621 ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg
     7681 tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat
     7741 gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt
     7801 ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc
     7861 tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc
     7921 agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc
     7981 aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt
     8041 tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat
     8101 gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg
     8161 tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga
     8221 gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc
     8281 ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa
     8341 aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta
     8401 cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat
     8461 ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac
     8521 tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc
     8581 atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc
     8641 ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta
     8701 cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa
     8761 tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa
     8821 gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata
     8881 cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca
     8941 tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat
     9001 agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa
     9061 aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc
     9121 atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga
     9181 acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac
     9241 ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt
     9301 tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg
     9361 acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta
     9421 gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt
     9481 atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc
     9541 agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat
     9601 caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg
     9661 tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat
     9721 caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta
     9781 tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt
     9841 tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt
     9901 agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc
     9961 gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga
    10021 attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca
    10081 aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc
    10141 agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc
    10201 attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa
    10261 atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat
    10321 agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc
    10381 gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg
    10441 catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat
    10501 cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac
    10561 tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat
    10621 tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc
    10681 tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa
    10741 atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc
    10801 aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg
    10861 tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa
    10921 atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg
    10981 tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc
    11041 tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt
    11101 ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga
    11161 gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa
    11221 gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg
    11281 aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa
    11341 aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc
    11401 agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat
    11461 gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt
    11521 tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga
    11581 taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga
    11641 attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt
    11701 gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg
    11761 ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca
    11821 gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac
    11881 gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg
    11941 ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca
    12001 acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat
    12061 catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga
    12121 gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt
    12181 acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc
    12241 aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc
    12301 tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag
    12361 cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa
    12421 acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa
    12481 aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg
    12541 agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt
    12601 gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg
    12661 gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt
    12721 agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat
    12781 ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag
    12841 cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa
    12901 gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat
    12961 gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag
    13021 caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt
    13081 cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct
    13141 ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca
    13201 cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag
    13261 gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag
    13321 aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat
    13381 ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa
    13441 acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag
    13501 gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat
    13561 ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta
    13621 ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc
    13681 agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc
    13741 gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt
    13801 cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag
    13861 aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg
    13921 gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg
    13981 cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa
    14041 atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta
    14101 ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa
    14161 ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta
    14221 tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat
    14281 cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa
    14341 atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa
    14401 ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa
    14461 acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt
    14521 gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca
    14581 aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat
    14641 gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat
    14701 tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga
    14761 ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg
    14821 gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa
    14881 gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa
    14941 aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc
    15001 tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata
    15061 ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa
    15121 acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag
    15181 atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc
    15241 tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata
    15301 tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga
    15361 aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg
    15421 caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg
    15481 tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata
    15541 tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt
    15601 tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac
    15661 agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca
    15721 ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac
    15781 tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa
    15841 aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa
    15901 acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat
    15961 ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag cctttggtga
    16021 aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt
    16081 ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag
    16141 ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca
    16201 tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag
    16261 tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag
    16321 aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag
    16381 aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag
    16441 cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat
    16501 ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt
    16561 gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac
    16621 aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa
    16681 taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc
    16741 ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata
    16801 gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg
    16861 attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc
    16921 attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct
    16981 tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct
    17041 ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg
    17101 gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg
    17161 ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata
    17221 tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta
    17281 attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga
    17341 aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt
    17401 gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa
    17461 gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta
    17521 ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc
    17581 tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt
    17641 ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca
    17701 taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat
    17761 aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta
    17821 cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg
    17881 ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt
    17941 gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat
    18001 gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta
    18061 tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat
    18121 cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa
    18181 aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg
    18241 atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga
    18301 ggaatggggg ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga
    18361 cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc
    18421 ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa
    18481 ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg
    18541 gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga
    18601 aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt
    18661 tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt
    18721 gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa
    18781 catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg
    18841 atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc
    18901 aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg
    18961 ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact
    19021 tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg
    19081 atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag
    19141 atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta
    19201 atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt
    19261 ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg
    19321 gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc
    19381 atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg
    19441 aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga atcagcgtaa
    19501 tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg
    19561 tacaagatct accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag
    19621 tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac
    19681 gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca
    19741 gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa
    19801 caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa
    19861 cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt
    19921 gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa
    19981 atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa
    20041 gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg
    20101 tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg
    20161 cggtttatgg aaaagaaaaa tctaaatccg ataaacaatc gcaaaaaagt aggtatcatt
    20221 ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc gtatatcgtt
    20281 attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga
    20341 agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca
    20401 gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat
    20461 aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat
    20521 ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg
    20581 tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc
    20641 gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg
    20701 atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat
    20761 gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt
    20821 aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg
    20881 gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta
    20941 caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg
    21001 acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact
    21061 ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg
    21121 gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc
    21181 gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat
    21241 gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt
    21301 tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat
    21361 aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta
    21421 aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg
    21481 gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca
    21541 atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca
    21601 actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa
    21661 tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta
    21721 cctggataca atgtatcggc aaaaaccgga acagcccaaa tcgcttctga tacaggggga
    21781 tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat
    21841 ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg
    21901 gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat
    21961 tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg
    22021 gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt
    22081 aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg
    22141 atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat
    22201 atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt
    22261 gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta
    22321 gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc
    22381 cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca
    22441 aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc
    22501 cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg
    22561 ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc
    22621 gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat
    22681 gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct
    22741 tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt
    22801 aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa
    22861 tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta
    22921 cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt
    22981 aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga
    23041 tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga
    23101 atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca
    23161 agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca
    23221 ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat
    23281 tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa
    23341 tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg
    23401 cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg
    23461 acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc
    23521 atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct
    23581 acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg
    23641 agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa
    23701 caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac
    23761 gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag
    23821 ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc
    23881 atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag
    23941 aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc
    24001 ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc
    24061 ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact
    24121 ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag
    24181 aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac
    24241 gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc
    24301 aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc
    24361 taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata
    24421 catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa
    24481 caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg
    24541 attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt
    24601 tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca
    24661 gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg
    24721 aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc
    24781 aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt
    24841 ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga
    24901 tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc
    24961 cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga
    25021 tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat
    25081 gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc
    25141 gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt
    25201 aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat
    25261 ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc
    25321 aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat
    25381 gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat
    25441 attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg
    25501 gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg
    25561 ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt
    25621 cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag
    25681 acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa
    25741 gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa
    25801 aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt
    25861 cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac
    25921 aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag
    25981 attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa
    26041 aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt
    26101 ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag
    26161 ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt
    26221 atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca
    26281 tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta
    26341 cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca
    26401 aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa
    26461 aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa
    26521 atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat
    26581 taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt
    26641 acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag
    26701 ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag
    26761 atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta
    26821 gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg
    26881 cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat
    26941 cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag
    27001 ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt
    27061 aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct
    27121 attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta
    27181 ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac
    27241 atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg
    27301 aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca
    27361 agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt
    27421 aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt
    27481 taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct
    27541 tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt
    27601 cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta
    27661 cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa
    27721 tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct
    27781 ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac
    27841 aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga
    27901 atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt
    27961 gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag aatttccagt
    28021 agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat
    28081 ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc
    28141 attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt
    28201 ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg
    28261 gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag
    28321 tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat
    28381 ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat
    28441 ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt
    28501 ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg
    28561 ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa
    28621 atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca
    28681 cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat
    28741 atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa
    28801 gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa
    28861 tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg
    28921 gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc
    28981 gttttgcagc agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct
    29041 caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg
    29101 aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag
    29161 gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat
    29221 tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa
    29281 tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt cttaacatca
    29341 caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg
    29401 cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg
    29461 aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta
    29521 gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg
    29581 atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac
    29641 gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag
    29701 aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc
    29761 caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg
    29821 cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa
    29881 gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga
    29941 gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat
    30001 ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta
    30061 aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa
    30121 gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt
    30181 gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct
    30241 gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat
    30301 gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa
    30361 ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt
    30421 gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa
    30481 aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc
    30541 tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc
    30601 aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca
    30661 tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga
    30721 aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac
    30781 agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga
    30841 accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac
    30901 cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac
    30961 aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg
    31021 tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt
    31081 gtttgatctg taagaggagg aactacgatt tatactattg cactattact tcatttactt
    31141 caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt
    31201 ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc
    31261 agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca
    31321 atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc
    31381 catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa
    31441 attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg
    31501 gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt
    31561 gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc
    31621 atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat
    31681 tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa
    31741 tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc
    31801 tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag
    31861 gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa
    31921 ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca
    31981 gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg
    32041 tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct
    32101 gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc
    32161 caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt
    32221 aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga
    32281 cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag
    32341 ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac
    32401 attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca
    32461 tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga
    32521 tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac
    32581 atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa
    32641 aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc
    32701 aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg
    32761 aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt
    32821 tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag
    32881 aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa
    32941 atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg
    33001 aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat
    33061 tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat
    33121 gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt
    33181 gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat
    33241 taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa
    33301 aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta
    33361 gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat
    33421 tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac
    33481 catatgttcc aggatgggat acacatggat tgccgattga acaagtattg gctaataaag
    33541 gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac
    33601 tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg
    33661 aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta
    33721 aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa
    33781 gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt
    33841 ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca
    33901 tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat
    33961 ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag
    34021 aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac
    34081 aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt
    34141 taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg
    34201 gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact
    34261 caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa
    34321 atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta
    34381 ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac
    34441 aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag
    34501 agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt
    34561 gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg
    34621 gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg
    34681 gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc
    34741 caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt
    34801 ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata
    34861 tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg
    34921 tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg
    34981 gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca
    35041 aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg
    35101 ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata
    35161 cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt
    35221 acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag
    35281 aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga
    35341 acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata
    35401 ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg
    35461 tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat
    35521 tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg
    35581 caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct
    35641 taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac
    35701 taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg
    35761 cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc
    35821 cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa
    35881 cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc
    35941 tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat
    36001 tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt
    36061 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
    36121 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
    36181 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
    36241 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
    36301 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
    36361 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
    36421 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
    36481 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
    36541 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
    36601 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
    36661 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
    36721 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
    36781 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
    36841 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
    36901 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
    36961 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
    37021 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
    37081 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
    37141 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
    37201 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
    37261 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
    37321 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
    37381 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
    37441 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
    37501 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
    37561 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
    37621 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
    37681 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
    37741 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
    37801 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
    37861 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
    37921 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
    37981 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
    38041 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
    38101 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
    38161 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
    38221 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
    38281 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
    38341 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
    38401 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
    38461 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
    38521 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
    38581 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
    38641 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
    38701 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
    38761 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
    38821 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
    38881 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
    38941 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
    39001 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
    39061 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
    39121 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
    39181 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
    39241 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
    39301 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
    39361 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
    39421 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
    39481 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
    39541 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
    39601 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
    39661 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
    39721 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
    39781 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
    39841 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
    39901 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
    39961 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
    40021 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
    40081 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
    40141 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
    40201 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
    40261 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
    40321 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
    40381 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
    40441 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
    40501 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
    40561 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
    40621 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
    40681 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
    40741 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
    40801 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
    40861 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
    40921 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
    40981 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
    41041 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
    41101 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
    41161 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
    41221 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
    41281 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
    41341 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
    41401 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
    41461 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
    41521 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
    41581 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
    41641 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
    41701 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
    41761 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
    41821 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
    41881 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
    41941 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
    42001 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
    42061 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
    42121 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
    42181 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
    42241 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
    42301 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
    42361 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
    42421 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
    42481 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
    42541 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
    42601 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
    42661 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
    42721 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
    42781 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
    42841 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
    42901 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
    42961 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
    43021 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
    43081 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
    43141 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
    43201 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
    43261 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
    43321 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
    43381 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
    43441 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
    43501 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
    43561 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
    43621 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
    43681 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
    43741 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
    43801 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
    43861 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
    43921 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
    43981 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
    44041 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
    44101 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
    44161 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
    44221 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
    44281 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
    44341 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
    44401 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
    44461 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
    44521 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
    44581 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
    44641 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
    44701 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
    44761 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
    44821 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
    44881 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
    44941 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
    45001 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
    45061 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
    45121 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
    45181 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
    45241 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
    45301 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
    45361 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
    45421 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
    45481 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
    45541 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
    45601 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
    45661 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
    45721 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
    45781 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
    45841 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
    45901 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
    45961 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
    46021 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    46081 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    46141 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    46201 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    46261 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    46321 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    46381 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    46441 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    46501 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    46561 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    46621 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    46681 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    46741 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    46801 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    46861 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    46921 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    46981 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    47041 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    47101 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    47161 gttcctcata atgaattttc taggaggaac tgaaatgatt acaattgcca gtgcaatgca
    47221 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    47281 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    47341 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    47401 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    47461 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    47521 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    47581 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    47641 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    47701 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    47761 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac
    47821 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    47881 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    47941 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    48001 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    48061 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    48121 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    48181 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    48241 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    48301 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    48361 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    48421 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    48481 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    48541 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    48601 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    48661 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    48721 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    48781 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    48841 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    48901 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    48961 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    49021 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    49081 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    49141 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    49201 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    49261 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    49321 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    49381 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    49441 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    49501 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    49561 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    49621 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    49681 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    49741 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    49801 caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg
    49861 ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt
    49921 tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt
    49981 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
    50041 aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca atccctaatt
    50101 cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat ccatttttta
    50161 agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat aattcttttt
    50221 gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa attttcctat
    50281 ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat gttttattaa
    50341 tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga agttcagcaa
    50401 atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac tgcgcagaaa
    50461 ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa tatttatctt
    50521 cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga tcaaacggaa
    50581 ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta aacttaataa
    50641 cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat cccactctat
    50701 ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac gtatcttcgt
    50761 attttctata agtgatagga gatactgttc cttctttata aagttgcatc caatcctcaa
    50821 agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc aagttatatt
    50881 ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat tttcttatct
    50941 tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta tttccacgtt
    51001 tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg aactataaag
    51061 aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc ggcgcaagga
    51121 tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc aatcagaggt
    51181 gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt ttagattatt
    51241 tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc tagatgtcat
    51301 attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa atgagatatt
    51361 ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat cgttttttct
    51421 aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg tgaatagcca
    51481 gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca tgctaacaat
    51541 tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc gttatcccat
    51601 gaagcgctgt taattcgaag taaacaattc ttataaagat ttttatacat atctatttta
    51661 ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc aatgagttgg
    51721 tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt ttctaatgat
    51781 tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt tttagaccca
    51841 aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc gccttcaact
    51901 attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat agttacctcc
    51961 tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt gtataggttt
    52021 tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt tacttacttt
    52081 actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc ttgaactaat
    52141 ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt ttaagtcctt
    52201 cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac ttttatcatc
    52261 gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa cagttccttc
    52321 aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct cagtagctgg
    52381 agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg tctctaaaaa
    52441 ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt gagtatcaga
    52501 tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa gcgagaaacc
    52561 tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct tatttgtttt
    52621 tttgataaat cttacaatta acatgattaa acctataata aataaaacaa taccagtaaa
    52681 accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat attataaacc
    52741 aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt catctaacaa
    52801 ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg aattattatc
    52861 taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat tccaatattt
    52921 ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc cgcgactttc
    52981 gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt tttttaattg
    53041 tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt gattaactaa
    53101 atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa tcaaggtttt
    53161 tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc tgtgatatga
    53221 ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg ggctttttta
    53281 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    53341 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    53401 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    53461 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    53521 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa aatgagaggc
    53581 gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt
    53641 ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat
    53701 ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac
    53761 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    53821 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
    53881 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa
    53941 tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga
    54001 taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc
    54061 gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac
    54121 aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac
    54181 tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa
    54241 atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct
    54301 tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt
    54361 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
    54421 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
    54481 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
    54541 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
    54601 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
    54661 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
    54721 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
    54781 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
    54841 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
    54901 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
    54961 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
    55021 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
    55081 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
    55141 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
    55201 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
    55261 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
    55321 gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat
    55381 tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct
    55441 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
    55501 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
    55561 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat
    55621 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
    55681 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
    55741 agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg gggaagcagg
    55801 caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct tgatgaagct
    55861 gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg aatagaaaag
    55921 agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt tctctattgg
    55981 gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta gagtaaatat
    56041 catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt caaatgcaag
    56101 gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag agtatattct
    56161 attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag ctgaccatct
    56221 gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt ctgttgaata
    56281 gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag cattaagttc
    56341 aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg tactcaatat
    56401 aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca aaataaacac
    56461 caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa gaattagtaa
    56521 ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa
    56581 aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc aattttggac
    56641 agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga gttttcttca
    56701 ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa gctatctact
    56761 tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc aagaatactg
    56821 ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa gatgttaaaa
    56881 aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta
    56941 agcaaaaagg aaatatgaaa gttatgggag ttctaggggg aaaagaaagc cgagcttatg
    57001 aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt accttttcaa
    57061 tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca tttattgaaa
    57121 tgtggttgcc agaaactgcg atccgtatgg aaattgatca actgaacaga caacagagac
    57181 tttttggtga tgacaatgaa tagagctgaa gcgctaagaa tagggacggc aattgctaat
    57241 tgctggtgga aatactataa accaatcatc ctaagccaac aacatattga caagcaaaaa
    57301 gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa agaaaacaca
    57361 tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa tatttgattg
    57421 atcctgattg gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc
    57481 ttttgacgaa tgaagaacta gcttgggatc aagatgattt aggcaaccat gtaactattt
    57541 gggatcatgt ttatcttatc catctatcga agcattcgaa taaacctgaa tatatttacg
    57601 tcatcgaaga tggcttgatt gatgcgctag aagagtacga cagagataac ttgattgata
    57661 tctcttatta cggaccaggt aagaaataca ttgctgaaat ggaggcagaa tttgatgagt
    57721 gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc
    57781 aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat tatgaaaaag
    57841 aatgatccac aggcaacggt aactattaat gagtacaaac actacagggt tgtttctgga
    57901 actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga aactgggact
    57961 tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt tcctgtttta
    58021 gattatcgaa accaaccagt tgttaaacca aatgcaatgc aaatcaataa ctcattgaag
    58081 cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt tcaaggggaa
    58141 gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga gacgattcta
    58201 gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga atatgttaat
    58261 gagcagacag ataaattagg gctcttagct gataacgttg aaactattga acagttaagc
    58321 tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga attagataag
    58381 aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg aaaaagttca
    58441 aaggaaaaga cataagcgca accatcaatg aggaagtgaa cattgatcga ctaaagacga
    58501 tgtatgacgg ctacgatggt gatcgagtca ttgaaattcg ttttattgat cctagacgtt
    58561 tcaccgtaca gcaacgaaac ttcatctatg cgctgatagg cgatattttt atcgatacag
    58621 gcatgccaac ggacttctgg aaggaattct tctactttcg ttttgaaggt gtcacagggc
    58681 gcaaaataag cctgaaagac gaatcgaata caactgtgag tgatgccaat gtcttagcaa
    58741 atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc tatgagattt
    58801 tacctgcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc tgctgcatct
    58861 gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga cgaaagcgca
    58921 aagaagttga tcatgcagag tacacatttg cagcactctg cagaatccat cacacagaga
    58981 agcacaaaat aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg atcaaattaa
    59041 accagaaaac aatcaaaaag ttaaatattg gagggtaaaa atgacagaac atcgaagtta
    59101 ttatgcgatt ataccagcca acgtaaggta cgacaaaaga cttaaaccaa atactaagtt
    59161 gttatacgga gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga
    59221 gtactttgca gatttatatg gtgtgaataa agagaccata tcgcgatggg taagtgattt
    59281 gattaagttt ggatacttga atcgggaaat catttacaaa gagggtacca atcaaataat
    59341 caataggtac ctacgaatta atcaataccc tattgacgaa aaacgcaata cccctattga
    59401 cgaaaaagtc aaagataata atacatctat taataataca tttaataata caaaagaata
    59461 tataagagag ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg
    59521 agagtataaa aatgttcttt tgtcagatga gcagatggag aaactcaaaa cagaattccc
    59581 taatgattac caagagcgaa tcgaacgact gtcagagtat tgtgaatcat ctggtaagac
    59641 ttataaaaac tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa
    59701 gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg aatgggcaat
    59761 cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac aatcaaaagc
    59821 accattctaa gaggtggaaa aatgaagatc gattatctag aactaattaa tgaaatagcg
    59881 aattataaaa agggcgagga attagacgtc ctgagagacg tatatgatca actcgaagaa
    59941 gctggaattg aaggaattaa gaatgatcgt tcgagttgga gtaaactcag atactatttc
    60001 gcactctata tcgatggaac gcaattaaga aatttagcat ataccaaatt actattcatt
    60061 gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta aacaagatgg
    60121 acctaaagac atttacagca cagatcgaat taatgcatca agaagcttta agacaaagtg
    60181 tgtcgtacga agacaagtgg ctcaacacat ttcatggcgg acgtgagagc gcacttgatc
    60241 aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc aatgcagcga
    60301 attatcgaat tgacttattc agaagattgg caggaagata aaaaaatagt tgcagaagtc
    60361 caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc gagaaagatt
    60421 gcaatctggc atggtgatcg aattctagta acgggtaccg ctaaacagtt atctgaaatt
    60481 actggtctga gcaaaaatat tatttgggat agagctagga gcttatggat tgattcaaaa
    60541 ggaagacagt ttaggtatgt ggaggagaaa taatggacga actaatcgcg aaagtagagc
    60601 agtgggcaaa agataaggga ttggatcaag cggatttcag caagcaaatg ttaaaaacgg
    60661 tcgaagaaat cggggaagtt gccgcttctc tagctagaaa agatgaacat ggtttaagag
    60721 acggaattgg cgatgtagta gtaaccttga tcatcttggc aatgcaaaat gatatggatt
    60781 tatacgagtg cttggatttt gcttatgacg aaatcaaagg tcggactgga aagatggtca
    60841 atggtgtttt cgttaagtcc agtgacctga aataattcca ttaatggaag aaataactga
    60901 ataggaggat agacatgatt gatgcaaaac aagtgcaaaa acagaaagat ggaatgttga
    60961 tgtttgaagc atatgttcta ccatttctta atcaatttga agttttggaa tgctctgcca
    61021 gcggagaaga attggagtat gttgtaattc gtgaaaccaa agaaaacgtt cagaagctaa
    61081 acgaatttct ttgcactatt aactgttggg atatgatagc tccaggattt ttatgtccag
    61141 ctatgggaga atttttggaa tattgtcgtc ttgaagatgc aggcactttg gatttagctt
    61201 atctggtata caactaccta aacatcaaca cagatcattt gtggtttggc actgccgaaa
    61261 gaaaatgggt agtccgctaa ccaccaatat caatgaaaaa aggcggtttc ctgaaagtgg
    61321 aattgccgcc attgctgtaa ggttctttac gcgactcaag gtcacagaaa tataaatgca
    61381 aatctctttt gaagtgatcc aaagctctcg gaaaatggaa gttttttgtt tcaaagaata
    61441 aatgagattt gcgtcaagaa cctgagagtt atttccaaaa aagcttccaa agaaccgaat
    61501 gagctaagga catcatgtgt aaacacataa tatagctctc ctttcacggc taatcataac
    61561 atgaaaaata ttcgatgtca gtaggaaaaa aggtgaatta tgaaaagaat attaaattat
    61621 cccggcagta acagattctg caattaaact ccgaagctga tgtatcagta tcaactgtag
    61681 aatattatga gcgtggagaa agtcaagcag tcacaaatgt aaaaatcgat aacgtcaacg
    61741 gtcttatctg tttagatgaa ggcgatagta tgaatgaaat tgctgcagac catgaattat
    61801 cagaaactgc tttaattgat caaatcgaat tagctagaca atactttgtt ccatatgcag
    61861 atacctggtc caagcataag aatgacatca tttttccaga ggaacaatga gagcaactga
    61921 tccagtaatt atccttgagg aagccaaatt tatttggact cacgaagaga tagagcaagc
    61981 acgcttgctc ttttctcaag gagttaagcc gagcaaagta gctgaaataa tgggtcaaaa
    62041 gattcttgat gtcggattgc ttttgctcca tctagcagaa aaaaatttga tttgaggtga
    62101 aaatgatgat tcaacttgca ggcatccaaa cagggaaaat ttatttttct ggagaaagca
    62161 aaagcgaagc caaccaatgg ctattgaaaa cttacacgaa caataagaag ctaaggaaaa
    62221 aatatcctga tttgttgcta aaggatgatc agattatgcc ggaaccgatg attttgaccc
    62281 gtaaggaggg aagttgaaac taaaaaacca gccgtgtggg ctggagttgc tatttctttt
    62341 taataatgag tttttctaaa tttaattcat tctcacgagt ttgcgtccaa taatcagaat
    62401 aatattttaa ttgtttaaga gtaggaatgt cgtcttcggt aaaattagaa aataattctg
    62461 gagaattatc tttttttaat tgttctaatc ctgattcttt aaaacgttgt tcaacgagtt
    62521 ttactaaatt gttgatattc atgataaatc acctttttaa atttaatttc gatttgatta
    62581 atcgatacaa gtattatatc aaataaaaaa gctgatttag ggggtcttac aaaaacacta
    62641 aaattttttc aaaaagagga ggaacggtgg tgaaagtcag agaacaattc aagatagtga
    62701 gcgacgaaga taatgtttac aaaataaaga tcgttaatat taatgaatat cgagaagcag
    62761 atcacaaata tttggttgac ttatacatga acgataaata tttgggagaa ggattttgtg
    62821 gagattcact ttttgatgat gagcgaattt tgaaggagga aacagaatga tccacgaact
    62881 aaaaatacta ccagaatatt ttgaagcagt gacgagcgga cgcaaacaat tcgagatccg
    62941 caagaatgat cgtgacttca aagttggtga tcagttaata ctacgtgaat ataaaaaata
    63001 cattaaacat gaattacctg gactaacgag accaacattt agttacactg gtgactcata
    63061 caaagcagag atcacttata ttactgatta catgcagaag tccggatatg tagtgttggg
    63121 gattaaaggg gtgaattaaa tgaaagtata catcttagct atcacagagg gcacatggat
    63181 gttcccagtc ggttccggaa aaatctataa atcaaaaaca gctgcttata aagcatttga
    63241 aaaatacaaa aaagaaaacg gtggcggcac taacgcaaag attctagtgg ctgataactg
    63301 gcatgaagaa ggtgaacgta attgatttac ctgttcattt tcgtggtact aactattttt
    63361 atgctagtaa tcttattcaa aaaaagatga gtaaagacga acgaaaaatt ctggccaact
    63421 tttatttagt attaaaaatc gaacaaaatg tgttttcgga aaggagaatt gtatgggaaa
    63481 aacaaaatca aagattaaga agaaaaaacg acgtttggaa caaaaggcaa tccagaatgg
    63541 aacggctaag aaaaaataaa aaagctaccc ctttcgagat agcagcgcaa tactattcta
    63601 ccataagggg tggcgtttgt gagatttcat tggttaaaag attaccaaga actagatgag
    63661 cagattcttt acttaaagtg gaatcttaat aaaagtaagc ttgaattgaa tcgatgggtc
    63721 tgtggtgatt tagcaaacgt ccgcatcgaa aagaattcga gaacttcttc tttagaagag
    63781 aatatccaaa agatagaaaa tgaattggaa ttgttgatcg agcaaaaaga agaaatgtta
    63841 ttgctgatag atagcttttc tggtatcgat aatcaaattg ttaagatgaa atatgttgat
    63901 caaatgagct tagaagatat tgctgaatct gttgggtata gctcatcgta tgtcagacag
    63961 cgacatgcag aaattagaaa gacattgaac tttttagatg agtatgaaca cagacaagct
    64021 gaccgattga aaaaagaaaa cgaaattgat ttttacaata gcgagaagta caaagaacaa
    64081 ctctctttat tctaaaatta cactgttcac taaatgttcg gtcattgtag ctatgtaaac
    64141 attgttagca gcatgatatt ctattagtgt caaaaaaata tgaaagagcc aagatatccc
    64201 aaccgtttta ttaattggta tctgtggctc ttttattctt ttaattagta gttggcataa
    64261 agttatttcc atttataaat tgatcagcta aatcagcatc ataatcagcc gcgataaatt
    64321 gcgctgctga tttaataaat gcatccatat catgaacatc tttattagta tgtctacgaa
    64381 catagtgcgt ttcatcatta ccaatccaag tagcagcttt ggctaatctt tggagtttag
    64441 gaaaatcatt caaataattt tcaattgttt tacctaataa catttttttt attttatctt
    64501 catcctctga atttttagat attgcataat ctttaattag aaattctaga gcttttctat
    64561 aaccaactcc agcaatttgt tccaattgct cagatttagc ttgtgtggct tgagtataaa
    64621 tatcaacaaa tgtaggagaa actttttcaa tattttctgg taaatctatt ttaatcggcg
    64681 gacggtaata atctttaatt aaaatatttt cagaatatcc ttcatcttca ttaaacgtat
    64741 ttataaattg taaactataa aatttgttgc actcatcatt tatacactgt actaatacgc
    64801 caaagattct atcattatcg aacgcactat atgatgaaac cccaaaatac acatacggag
    64861 tcattatatt tccgcaatgc ggacaactat ttggcaaata aattgtctct gtattttgac
    64921 gatcacgata cttaatatta acttcttgaa gcattaaaac acctcgcatc ttttttataa
    64981 atcataccac aaaatatctg ataggagagt aaacaaatga atgattttca tgaagcggta
    65041 cttagtattg aagtagaatc aagtttggct aaagtttata agaaagcaat tgaagcagag
    65101 aatagtccgt acagagaaaa ttggaatggt aatcatgcac atgtgcaagt ggagaatgat
    65161 gattacagaa ctggaatgaa tacgttagtt atttctttat tgtcacacac tctacccaat
    65221 ctgcaggaaa cgattgaatg gtatgagcga atgggcgcaa aagtaatccg tacaaattac
    65281 aaaggagaga attaaaatgg caatgatcaa aattagaaca tccattagtg gaacagagta
    65341 ttgggattca gaaaagaaaa ggacagtagt tgttccgaaa gatcaagaac caaattttga
    65401 agtaaaagaa gaaaaaggaa ttttgagtga tggcaaatca tttgttgctg tcaatggcga
    65461 actcatagct catagcagtg aaattcttga tagcgatgga aacactgctg ctaattttga
    65521 tggagacgaa gctactaatg atcagtccac tgaagatacg gatgaactgg acaatatgac
    65581 tgcaaaagaa ttgcgtgcat atgctaagaa acatggtatt gatattcctg gtgctatccg
    65641 tgcaaaaggc gatatcataa atcttattcg tgaagtagaa tgaaatactg tcagtttgac
    65701 ggatgcacga acaaaatagc aaagggtatc tactgtactg aacacaagag atcaagcaga
    65761 tcacgcaaga agaagcagca agcaaagtct gtttatcatc atgagaacaa accattctat
    65821 cgaacgcaag catggaaaga tatgcgtcaa tttatttatg aaagagaagg tggtcattgt
    65881 cagcggtgtg gtcagttcat atttggaaag agagcacacg tccatcacat tgtaccaatc
    65941 aaagacaacg aactgcttaa gcttgatcca aacaatctca tgctattatg ttcaaaatgt
    66001 catccaattg ttgaaaacga aacggaagac aaaaaagttt ttccttcgta tttcaattga
    66061 agccccccta ttcattttca aaattttttc gcgtggggag atagggtagc ggggagtcac
    66121 gcgcatcgtt aggtcaaatt tttcaaaaaa caaagggggg tgtatacaaa aatgacgact
    66181 aaagcgcaac gtaaagcgat tattgatgaa aaagtaagtg ctgaaaaagc tcgtatctta
    66241 gaaataatga atttgtctga tttgtacacc atcactcttg atccattaat cgaatcatac
    66301 ttggatattt ttgaaattta ccaacacaaa tatttattgt ggaaggaaaa aggatttccg
    66361 gagacgcaaa aattcacgaa taaatcagga gctactaatc aatcaaaaca tccattggca
    66421 caacaagtag aaacttgggc agataagaag atgaaagctc tagatttatt aggattaaca
    66481 aacaaagcaa aaactggtag actaattgct ggaggatcga ccgcaagaaa agatgaagaa
    66541 attacacgcc cagaagtaaa gccagtagat gaactagcag cgcacagaaa taaatggcgt
    66601 aagaaggcag gtgctgaaaa atgattgaac ctggtgtaaa ttatgctgat ttatttgcaa
    66661 aagaagtaag aaagaaacct aagaagtatc ctaagaccgt tcgtttagca atagatcgct
    66721 ggtatcagtg gaaaaaaaga aaagatattt ggtttgatgt agatcgcgcg aatgaaatga
    66781 tggattgggt agaatcattt attgttcaca caaaaggaga aatggtggga aaaccgtttc
    66841 ttttagaacc ctgggaaaaa tttatttatt cttggattta tggttgggtt aaagaaaatg
    66901 aaaaaggcca aattgtacgc gttactcgtg aggcttatgt ccagattcca aaaaagaatg
    66961 gtaaaacatt gattgctgtc ggttcattag gatatgcaat gtacggagaa ggggcgttat
    67021 cagttgattg ctatgcttgc gcatctgatt ttgctcaagc gcaatatgct gccaaacctt
    67081 ttgcagttac aatcttaaac aatccaatcc tacttgaagg aactaaaata tttagggggc
    67141 caaagggaac tgtttctagt attacttatg actatattca tgaagatatg gcttactcaa
    67201 ataaatttat tgttcaaacc aagaatattg ataatatcga gggatccaat ccatattttg
    67261 tattgaacga tgaattgcat aaacaggaga aaatggaaca gtatgacaat tttaagtctg
    67321 ctcagatatc tttaccacag ccattgatgt ttaatatctc aactgctggg aaaggttcgt
    67381 cgtctgttgg aatgcgtgta tatcgcgaag caaaagaagt gttgaaacgt gatgataatg
    67441 attcaaactt tgttctaatc tatgagccaa ataaaaatta tgattggacg gatagaaaag
    67501 tctgggaaat gtgcaatcct aactggggaa tatcagtcga tttatctgct ttagaatcgg
    67561 catttaaaac agcgcaacgg tcagctcatt ccaaagctga atttctaacg aaacacttgg
    67621 atgtatttgt gaacggcgcg gataatttct ttgaacagga tcaagtagag ccatgtttag
    67681 ttaccacaca agaacttggt gatttaagtg gcgaaccatg ttatatcggt ttagatttat
    67741 cacgtacacg agatttgacc tgtgtatctt taaacttccc aacatgggat gaagatggta
    67801 aagcggttct taaagtaaag caactttatt ttattccgaa tgaagattta gagtttcgag
    67861 aaaaagaaga taatgttcct tacagtgatt tggctgaaca aggtttcgtt gaattttgcg
    67921 acggaaaaat gattgatcaa gatcagattt tgcagtacat agaggactgc atgaatttat
    67981 atgatattca gcaagtgaat tatgatccag cgatgagtga caaacttgtt gagaaattag
    68041 aaaacttagg attggaatgc gttgaagttg ctcaatatcc taaagtattg aatgctcctt
    68101 ttgacgatgt cgaacggttg ttttatgaga aacgaattca atttgataat ccattattcc
    68161 tttattgcac tttgaacgtt gtagcaatta ctaacatcaa tggacaaaaa gcgccaagta
    68221 aacgtcaatc aaagaaaaag attgatgggt tcgtggcgtt tttgtgtggt cataaggaaa
    68281 cgatgaatca aatgacagat attgattcag atgagttaga tgattaccta agttccatct
    68341 atagataaat agaaaggcgg tgaaaaaaat tgaaattacg tgatcggtta tcgaatgcag
    68401 tctattcttt tatggaaaaa cgtggatata tcgaggatat ttttggacac tacacacgtt
    68461 atggtcaaag atatgtgacg gattcatcta tcatggaatc ttctgatgtt tatgaattag
    68521 ttcaagacat atctaatcag gtggcattgg ccacaccaat tgttattggt cccgacggca
    68581 acgaagtcaa agatcatcac ttactaaata ttttgaagag tcccaatgat tatttgacag
    68641 gattcgaatt taccaaattg gaaacaaata ctttattgat caatggtgag acattcccat
    68701 taacggatag ggatcagctt catttggcgt acggtgtaac aactaaaatc aatgaacgac
    68761 ttcaagaaga atttgaaatg aatggtcaaa aaatacctgg tcaaatgatt cgacacatta
    68821 agaacatcgg aactgattca ttaaaaggtg ctggaataat tgatcttgca agaaacactc
    68881 tagaaggcgt tctgagcgct gaaaaagttt tgacggacaa atatactaaa ggcggtttac
    68941 ttgcgttcat gcttaaacta gacgcccaca tcaatccaaa taatagcgcc caaacaaaaa
    69001 ttgtcaaagc tatattggat caactggaag gaacgcaaaa tgagagtgat cattctgtta
    69061 agatgattcc tttgggaaaa ggatattcca tcgagacatt aaaaagtcct gttgatgacg
    69121 cggcaatttt gaattattta ggtgtttaca aaaaagactt aggaaaattt ctaggaatca
    69181 atgttgatac gtatcaatcg ctgatgaaga cagatattga aaaagcgatg atgtatctgc
    69241 acaacaaagc aatcaaacca atattgaaaa acaagagcga acattacacc gctctttttt
    69301 ttatgcctaa ttctggctat agagtggaat ggaaaattaa tattttggat tttgttcctt
    69361 actcaacaaa aacaaatatt ggctacaaca tcgttcgtac cgggattaca agtccagata
    69421 acgtggcaga aatgcttggt tttcctaaac agaatactcc agaaacacaa gctatctata
    69481 tttcaaatga cttatctagg attggccaga aaaatgcaac agatgattcc ttaccaacga
    69541 atgatcaaga cttgaaagga ggtgatggaa atgaagaaga aggaaattcg cacgattgac
    69601 atcaccaatc tttcaacacg ttccgatgaa gaaactcata cgaggaccat tagcggctac
    69661 gctgctgtat tcaacagtca aacactttta tgggatgact taagcgaagt gattgttcct
    69721 ggtgcattct caaaggcaat tagtaattcg gatgttcgtt gtttgttcaa tcacgattgg
    69781 tccaatgtgc taggacgtac taaaagcgga acccttcgcc ttgaagaaga tgaacgtggt
    69841 ttgaaattcg aagttgattt accggacaca acggtagcaa gggacttagt taaatctatg
    69901 gaacgaggag acatcaacca atgtagtttt ggttttgtgc cgactgaaga aacgtgggac
    69961 tacaattctg aaccaatgct tcgaacaatc catgaagtgg aattatacga ggtttctatt
    70021 gtgcctttgc cagcatacga agatacagaa gctgcattaa gaagtcgtga tgaattagaa
    70081 aaaaacgtcg aagaaagaaa aaaattaatc aaaaaaatta atcaagcgct agaagcgtag
    70141 gaggaaaaat acatggataa agaattattg aaaaaaatga aggcacgtcg tgagcaacga
    70201 ttgactgaat tacgtgaaaa agttgaatca ggagaattac gcgaagcaga tttagaagcc
    70261 gtgaaagaag aaattgacag tgttattgat gaattgaacg gaattaaaga cgaattaggc
    70321 gcagattctg gaactgatga aacagacgac aatacagatg atcaatcgaa tagtacggat
    70381 tcagacgaaa gccgttctgg tgaagacaac gatcaagaag aagattcaga cagtgaagat
    70441 agttcagaaa atcgttctgg aatgattact caacagcaac gagatggatt acttggatca
    70501 attaagaacg gattggaggc acgtgcaaaa atgaccaaga aacaaaaaga tcaacaacta
    70561 cgaaaagcat ttgctaattt tgtagctgga aatatttctg aagcagaagc tcgagcttta
    70621 gggattgaag ctggcaacgg ttcagttact gtcccagaag taattgcatc tgaagttatt
    70681 acttatgctc aagaagaaaa tttacttcgt aaatacggaa cagtggtgcg aacatcagga
    70741 gatgtcaaat atccaattct tgtgaagaaa gcagatgcga atgtaaacaa gaaagagcgt
    70801 tcaactgata ttgctgaaac agctattcag tttgatgaaa ttttgcttaa tcctgccgaa
    70861 ttcgatgctt tggcaacagt aactaaaaaa ttattaaaaa tgtctggtgt tccagttgaa
    70921 gatattgttg tggaagaatt gaaaaaagct tatgtgcgta aagaaatcaa ttatatgttc
    70981 aatggtgatg acgctggaaa tgaaaatcct ggtgcattag ccaaaaaggc tgtagcattt
    71041 aaaaaacctt tagatctaac tgcttcaggt gctgggcaaa aattatatga tgcattaatc
    71101 gaatttaaaa atacaccagt gacagaagtg atgaaaaagg gacgctttat tattaatcga
    71161 gctgctttga ctgccattga aaaaatgaaa acagatgatg gatttccttt gttgcggcca
    71221 ttcacacaag cagaaggtgg aattggttac caattagttg gctatcctgt ggattggaca
    71281 gatgcagcag ataaaaaggg tgaaccagac acgccagttt tatattttgg cgatttttct
    71341 gcatttaaaa ttcaagaagt tattggtgcc ttggaaattc aaaaacttgt tgaaaaattc
    71401 tctggtaaaa atcaaattgg attccaaatt tacaacttgc tagatggtca attggtttat
    71461 tctccatttg aaccggcagt atatcgctac gaaattacaa aaccagttgg tggttaagat
    71521 ggaagagcaa actaaagaat tgtctttaga ggaaaaattc aaatcacata ttcattttga
    71581 agagggcatg gatgattctt tgctctcttt ttatttaaat atggcaaaag attatgtcaa
    71641 aacagcaact ggtggccaac aagaatatct tattttgatg gttgccggca ttgcctatga
    71701 atatagagtt tcagaggatg aactcgacaa agctatgaat gccatgacgc catttatcgt
    71761 gcaaggagcg attcaaaatg ccgaagagac agaccaataa tctcagatgg aaagccgaat
    71821 tgctagacat caaaacagga acagacggaa acgatcgtcc aactacaatt tacgaattta
    71881 agcgtctaat attctatgaa gaactcggtg tgacttctca agaaaaatat ttatcacagc
    71941 aagccaagac agacgttgtc agacgaatta aagtcagatg ggataaatcc atcacagaga
    72001 aattaagtgc gctcaaaatt gattctgtaa cgtataacat tactcgcatt tatacgaatc
    72061 ccgatgcaag agaaatggag ttgagtttag cttatgtcga ttagctttga tgaattgaaa
    72121 acagcgctga aatcaacaaa gttagcagtg ttcagagaca aagccagatt agggacgatg
    72181 tatccataca ttgtgtactc aaatgtgagt aacagcaaaa aaatggcatc cggtaaagtc
    72241 tataaaaaat taccttatta ccaagtctct ttttttacac ttggaacaga acaggattta
    72301 gttgttattg aacaagcgct gcaaaatgct ggcattccat attcagattt tacaggtata
    72361 caaggtgatg agaacgacga taccgtaacc aactattaca catatgtgag gtgtgtggaa
    72421 aatgccaagt aataagaatg gtttttctga aatatcggat tatttaggaa acctttcaaa
    72481 agttgatccg aaaaaattat cgttggaatc tttagaagaa gcagctaact tttatctaaa
    72541 gcagttgctt cctaatattc ctaagtcgct cttaaaaaag aaacatatgc gcgatcaaat
    72601 aaaagtagta gttgaaaacg atcaagtgaa agttcaattt gaagagacgg ccttttactg
    72661 gcgctttgcc gaaaatggca caacaaatca acgtgcccaa cattttgcaa gtggtacgta
    72721 tgaacaaaac aaagaaaaaa ttgaagaaat tatgactaga aaaatacttg atttatggga
    72781 agggtgattt taattgggaa aacaagatac tttttatttt gaaggattgg acgacatttt
    72841 aattgcgatg atggcaacgc cagattctgt tggaactgca ccgacttata gtgaagtttt
    72901 acgattgcca attgccacaa aaatcggtgt caaagggaat ggaacagcat tagaaaaatg
    72961 ggcatccagc aaaatgtttc gtcgtgtgtc gcgagaaaca aaacatgaac ttgcgttgga
    73021 tcacgtgggt attccgatag aagtgatgga tgaaatcaaa ggattagttg ctaaaagcgg
    73081 agtaacgttt agcaaaaata ccgcacgaga atttccgtac ttcgcctttg gatttattgg
    73141 aaatattgaa aatggcggta aaaaagcagt ttggtatcct aatacacaac tatccaatgt
    73201 gattgacgaa gagtatgcca ctgcagagga agaaacaaaa attgacgatg taactgcaaa
    73261 tctggtttcc atcggactga aatacaataa tgtgatgtat gcaagttttg attcaaatag
    73321 agacggagct tctctagaat tatttaataa attcattgct caacctgtat acgatgaaga
    73381 acagtggaaa acattggcta aagttggagg tgcgggttaa tggctcggtt atctgattat
    73441 ggaattcacg ttgaagactt aaaaaattct gctgttgtaa ttattcaagg cgcagaattc
    73501 cctatttcat ttactatgca aacgatggaa tttatagcag atgtgtatgg tggagattat
    73561 tcgcaatttg aatctgatat gaacgccatg ctatctaaaa aagaaggaca aatttcttct
    73621 gctaacttat cgcctagtga tctaaaaatc atgcgtgcct tgatttatgc catgctgcgt
    73681 actggtggtt tagaggaaga tccagaaact attttcaaat ttttgggaat gagtggagag
    73741 gtgttgtctg cttatagtac ctgtatggaa atttttgcta gccagacatt tcaggtggaa
    73801 gacctaaaaa aatccaagaa gccacaagac tttcaaaaag cgcaagcaaa aagaaagaaa
    73861 aacaaaaaga atcggaagag ataggaactc cttggagttt ttatatttac gttgctctca
    73921 ctctattgaa ttggagtgag agtttctttt taaagtctac acctaacttg tggctcaaat
    73981 cttatttaca gtggttacaa caaaacaccg attttgaacc acctcaatct gtaactatgg
    74041 ataaatcgcc ttggtggtag aaaggagcgc taagataaat ggctggtaaa gaatctgatg
    74101 tcgttcttaa ttttaaaacg aatggcgaag tcagttattc gaaaacaatc aaagaaatca
    74161 acaaagaaat gaacttagcc gctgccgagt acaaaaacca agtgtctgcg atggacaagg
    74221 atgcaactca aacagaaaaa ttgcgagcgg ctaaacaaaa attagaaaaa caattaggat
    74281 tggctgaaca acgatctcaa atgctgagag aggaatatga aaaatctgtc aaagaaaccg
    74341 gtgaatattc tgctgaatca gaaaaacttt ataaacaatt actcaattct gaaaccggag
    74401 aaaataaact tcgtacagca ttagaacaga cgaacgatgc acttaaagaa caaggtgacg
    74461 tttctgttga tacagcaaaa aaactccaga aaatcgaaga aacaggtgag aaagtaaaag
    74521 gcgttggtga aaaaatgtct gttggagtaa ccgcgcctat tgtagcggca ggagcagcag
    74581 gacttgcagc atttggtgaa gttgacgagg cacttgatac catcattaca aaaaccggag
    74641 caacaggtga tcaagctgat agactttcac agtctttcaa aaacgttggt tcaaatactc
    74701 atttaccttt acaaacggtt ggggaagcta ttggtgaggt aaatacacaa tttggattca
    74761 tggataaaaa actggaagat tcaaccaatt atctcctaca gtacgctgaa atcaatgata
    74821 cagatgtttc gcaatcagca atatctgctc gacaagctat tgatgcttat ggactagaat
    74881 atgatgattt gaattctgtc cttgatgtaa caacgaaaac atcgcagaat actggtcaat
    74941 ctgtagacga cttgatgcaa aaagcaattg atggcgcacc acaaattaaa caattagggt
    75001 tgagctttgg tgaaggggtc actttacttg gacaatttga gcaatctggt gttgattcaa
    75061 gcgcagcttt aagtagttta tctaaagcaa cagtggctta tgcgaaagac ggaaaatctt
    75121 taagtcaagg actagaagaa ctacaagaca aggtaaaaaa tgcaggttct gaaacagaag
    75181 ccatcaatgc agcagcagaa gtattcggga ctaaaggcgg tcctagaatg gccgatgcaa
    75241 ttcgtagagg tactttgaac ctggaagatt tagcaaaaac tgctggagaa agtgggggag
    75301 ctgtaggaga cacattcgat gctactctcg acccaattga tcaagccgat caagctatga
    75361 acaatgcaaa attagcaatg gctgacgtag gtgaagcagt ccaagtaagc cttttgcctt
    75421 tttttgaaaa agcaacttcc ttattacagg aattctctaa atggtggggg tctctagatc
    75481 aagatacaaa gaattggatc ataacaattg ctggtatagc ggcagcagtt ggaccagtac
    75541 taatcgttct tggttcactt atgggatctg ttacaaaaat cgttggggaa gttaaaagtt
    75601 ttataggagt ttggcaagga cttgctggtt tatttggaat gtcgggaggc tggtttgcat
    75661 tagcagttat agctattggc gcattagtta ctggactaat ttgggcatat aataacgtta
    75721 aatggtttca tgatggagtg aattctttct tcaaaggtgt ttcagatgta gctgttgaaa
    75781 tatttaattt tttaagtggt tattttggtg gatttttcga tggtgttata gcaaatttca
    75841 ataattttta taatgctgga aaacgaattt tcagtggttt tctagatttt atcactggag
    75901 tatttacagg gaattggtca aaagcatggc aaggtctagt tgatattttt ggaggtattt
    75961 tcgatggtat tgttgctgta gggaaagctc ctattaatgc catgattgga ttgatcaatg
    76021 gatttattgg tggattgaat aatataaaga taccaaaatg ggttcctggt gtaggtggaa
    76081 aatcattttc tatttcaaaa ctgccttatt tagcacaagg cggtcatcta atcaatggac
    76141 aagcgattgt tggtgaagct ggacctgagt tactgactgc caagaatggt aaaaccacag
    76201 ttactccatt atcagacgaa gaaaaacgca aagggatcag tggaagagtt tctggaggta
    76261 gcattgaaca gcatatacac attggcaaag tcgatgcaaa caatccatct gagttagatc
    76321 gtatgaatcg taaatttgct aaagcaaaca aacaggctat tcgtgatttg ggaggtgttc
    76381 ctatttgagt cgacaattta tgaatccaga tgaaccaaat tttatttgga aaaatcggaa
    76441 tgcagtaatt gatatgaatt gcatcattga ggcagagctt ccagatatta tgccgaacaa
    76501 gcgatacgaa acttacacga ttcagggccg aagcggagaa ttgacggaga cgtttgatga
    76561 ctatgaacct tttgatttgg aaatagaagg gatcactatt ccacattcga aactgagaga
    76621 agtcaaacga tggctcactg gtaaaagcca attaatcaca cataatgatc cagacaagta
    76681 tctagaggct atctgcagca tggataaaga agtccctttc gaaaacgagt ggggcttctt
    76741 ttatacattc gatgttactt ttcgttgtca gccactcaaa agaaaaatcg gtgaagcacc
    76801 taaaattttt cattctagtg ttatggatat ttttgatcat ggtgacgaaa ttgcacatcc
    76861 ctatctggaa atagaatcga acggaggaga tataacgatc gctgtcggtg ataaaagcct
    76921 aacaatactt aatacgttag ctgcaacaat cgtggttgat actcagcttg gaaaagcaat
    76981 acaagaaggt ttgaatttat ttacgcgtgg tgattggcca gtattgcagc ctgaatggaa
    77041 tcaggtaaaa atatccggaa aatttaaaga agtgcggttt tggaatagga gtgtgtatct
    77101 gtgacgcaag aatttattta tgcttataaa aaaatgccag atgatttgag cgtgaacgga
    77161 gcgtcgttag ttgattggga agatttgcca gaaatcaatc gtgtgctgaa tggccaatat
    77221 cgtttttatg gtaactattc aagaagcgga cagtatcgct cttatttaaa aaaaggaaat
    77281 tttataaaag cgaaggtacc agatggatca tggcaatatt ttgaaattta taatgtcaaa
    77341 aagaatttga cctccgtttc tgtaacagct cggcatattg gttttatggc caataagaac
    77401 tttattgtca agtcgtttac ggataacggc aatgggtctc agatcatgac caatcttaaa
    77461 aacagtttgg catttgatca aaaatttaac tatctttcga atgtagggac aacgcaccaa
    77521 tttacagcaa gacaagtcgc gccagttgaa gcaatcattg gttcaaataa cggtaatcaa
    77581 aatttgactg gcgtgactag cgcggaactt gatatggata actatgattt gaaactggta
    77641 aaacagattg gatcagataa tggttttcgg attgattttg gcatcaattt agaagcaatc
    77701 gaagaagaaa ttgatgaaga atcaattgtc aatagtttgt atttggttgg cggtgttcct
    77761 gataacgatt atgatgaaga caaagaacct atcgaatatg gttatttaga aattgatgga
    77821 gtaaccaacg agaatcgacg tattgccaaa cgtgaaaacg gagattgtaa aacagttgat
    77881 gaattgatca aatggggcaa aaccttattt gacaatgatc gtatccatga gccaaaagct
    77941 actcatacag tcagtatggt ggcgttggaa cacacactcg aatacggtga catgtatcaa
    78001 gagctagcgt ctttacactt tggtgatgtc gctcatgtta gggcgaaaga gctagatatc
    78061 gagataaaag agcgtatggt cgaatacact tatttcccaa cgcttgggaa atataaggat
    78121 attgttttag gtaatgattt atcactgtac acttcggcag taaatactca agcacaagaa
    78181 ttgaaaaaga aaatcgacaa tcgaacagaa acgttagttc aaaatgtgtt gaatgcgacg
    78241 gcatggatca ctggtaattc tggtgggcat gtcgtttttc gtccagaaaa agctccatca
    78301 gagattctta tcatggatac tccaaatgtg gcgaatgcaa agcgagtgtg gcgttggaat
    78361 ttaaatggct taggttattc agacaacgga gtaaatggcc cgttcggtat tgctatcaca
    78421 tcaaaaggag aaattgttgc tgacttcatc aaagtaggaa caataaatgc agaagtattc
    78481 gaaacttcct ttaatgctta tggtgatgtg ttaaaacttg taaaaggcac gcttcaaatt
    78541 tggaatgaaa acaaaaaaat catggagcta accaaaaaag gtatggaatt ctggaataga
    78601 aaagaatcca ttggaacgat cggaacaaca gattcagcag gaaatccatt tccaaatgct
    78661 gtaacaccta cacctcttga ggaaaattct ttggtaatac gtacaaatgg cgatggaaaa
    78721 tacattttaa tttctccaac tgcagaaaaa ggatttgtgt tattaggaaa cggaaaagca
    78781 tattatttcg gtgatttaga tattcaggga aaacttactg ttagaggtaa agaagtgata
    78841 ccaggacaaa atggcggtcc atctggcgga ggagaaacac ctggtgggta tcctgatgaa
    78901 ttaaaaacag atgcagaaaa gagagcttgg agaat
//
LOCUS       DAFFFQ010000004        71879 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000004
VERSION     DAFFFQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.004"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1722
                     /locus_tag="LQB69_000332"
     CDS             <1..1722
                     /locus_tag="LQB69_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL3524523.1"
                     /translation="NLYVNACPGSGKTRVLTRKIAYQAIKHDGSLKRIIAITYTNRAA
                     EEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYPFKMNLSRVSKGFTIIDDYTQRAYV
                     SQINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTMLEKNKEIDFDMILL
                     VAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQNNPQIQSMFVGDID
                     QAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRG
                     NNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYL
                     KKELPYCNFDAINISPIKVNNHSLFFKLAILYFTESGEKVRRRKLIANDILIILKNEF
                     GIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKH
                     YLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNG
                     KIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDK
                     IEFDYNI"
     gene            1922..2236
                     /locus_tag="LQB69_000333"
     CDS             1922..2236
                     /locus_tag="LQB69_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3524524.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            2257..2634
                     /locus_tag="LQB69_000334"
     CDS             2257..2634
                     /locus_tag="LQB69_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3524525.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            2644..3417
                     /locus_tag="LQB69_000335"
     CDS             2644..3417
                     /locus_tag="LQB69_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524526.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            3439..4842
                     /locus_tag="LQB69_000336"
     CDS             3439..4842
                     /locus_tag="LQB69_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3524527.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            5024..6208
                     /locus_tag="LQB69_000337"
     CDS             5024..6208
                     /locus_tag="LQB69_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3524528.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            6205..6495
                     /locus_tag="LQB69_000338"
     CDS             6205..6495
                     /locus_tag="LQB69_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524529.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            6492..6713
                     /locus_tag="LQB69_000339"
     CDS             6492..6713
                     /locus_tag="LQB69_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524530.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            6755..7534
                     /locus_tag="LQB69_000340"
     CDS             6755..7534
                     /locus_tag="LQB69_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HBL3524531.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            7601..8095
                     /locus_tag="LQB69_000341"
     CDS             7601..8095
                     /locus_tag="LQB69_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3524532.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            8151..8789
                     /locus_tag="LQB69_000342"
     CDS             8151..8789
                     /locus_tag="LQB69_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524533.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            8861..9256
                     /locus_tag="LQB69_000343"
     CDS             8861..9256
                     /locus_tag="LQB69_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3524534.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            9240..11693
                     /locus_tag="LQB69_000344"
     CDS             9240..11693
                     /locus_tag="LQB69_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3524535.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            11690..13717
                     /locus_tag="LQB69_000345"
     CDS             11690..13717
                     /locus_tag="LQB69_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524536.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            13714..14736
                     /locus_tag="LQB69_000346"
     CDS             13714..14736
                     /locus_tag="LQB69_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3524537.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            14753..15682
                     /locus_tag="LQB69_000347"
     CDS             14753..15682
                     /locus_tag="LQB69_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3524538.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            15927..16043
                     /locus_tag="LQB69_000348"
     CDS             15927..16043
                     /locus_tag="LQB69_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL3524539.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            16059..17978
                     /gene="tet(M)"
                     /locus_tag="LQB69_000349"
     CDS             16059..17978
                     /gene="tet(M)"
                     /locus_tag="LQB69_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3524540.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            18076..18264
                     /locus_tag="LQB69_000350"
     CDS             18076..18264
                     /locus_tag="LQB69_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL3524541.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(18322..18675)
                     /locus_tag="LQB69_000351"
     CDS             complement(18322..18675)
                     /locus_tag="LQB69_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524542.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            19206..19628
                     /locus_tag="LQB69_000352"
     CDS             19206..19628
                     /locus_tag="LQB69_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3524543.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            19625..19855
                     /locus_tag="LQB69_000353"
     CDS             19625..19855
                     /locus_tag="LQB69_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524544.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            20353..20553
                     /locus_tag="LQB69_000354"
     CDS             20353..20553
                     /locus_tag="LQB69_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL3524545.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            20580..21773
                     /locus_tag="LQB69_000355"
     CDS             20580..21773
                     /locus_tag="LQB69_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3524546.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(21841..23406)
                     /gene="guaA"
                     /locus_tag="LQB69_000356"
     CDS             complement(21841..23406)
                     /gene="guaA"
                     /locus_tag="LQB69_000356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL3524547.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23465..24388)
                     /locus_tag="LQB69_000357"
     CDS             complement(23465..24388)
                     /locus_tag="LQB69_000357"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL3524548.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24584..25189
                     /locus_tag="LQB69_000358"
     CDS             24584..25189
                     /locus_tag="LQB69_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3524549.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25497..26513
                     /locus_tag="LQB69_000359"
     CDS             25497..26513
                     /locus_tag="LQB69_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL3524550.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26654..27955
                     /locus_tag="LQB69_000360"
     CDS             26654..27955
                     /locus_tag="LQB69_000360"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL3524551.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28001..28663
                     /gene="deoC"
                     /locus_tag="LQB69_000361"
     CDS             28001..28663
                     /gene="deoC"
                     /locus_tag="LQB69_000361"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL3524552.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28684..29082
                     /locus_tag="LQB69_000362"
     CDS             28684..29082
                     /locus_tag="LQB69_000362"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL3524553.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29148..30230
                     /locus_tag="LQB69_000363"
     CDS             29148..30230
                     /locus_tag="LQB69_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3524554.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30442..32004
                     /locus_tag="LQB69_000364"
     CDS             30442..32004
                     /locus_tag="LQB69_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524555.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            31997..33130
                     /locus_tag="LQB69_000365"
     CDS             31997..33130
                     /locus_tag="LQB69_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524556.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33130..34086
                     /locus_tag="LQB69_000366"
     CDS             33130..34086
                     /locus_tag="LQB69_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524557.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34185..35351
                     /gene="deoB"
                     /locus_tag="LQB69_000367"
     CDS             34185..35351
                     /gene="deoB"
                     /locus_tag="LQB69_000367"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL3524558.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35375..36193
                     /locus_tag="LQB69_000368"
     CDS             35375..36193
                     /locus_tag="LQB69_000368"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL3524559.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36210..36917
                     /gene="deoD"
                     /locus_tag="LQB69_000369"
     CDS             36210..36917
                     /gene="deoD"
                     /locus_tag="LQB69_000369"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL3524560.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37215..37901)
                     /gene="gpmA"
                     /locus_tag="LQB69_000370"
     CDS             complement(37215..37901)
                     /gene="gpmA"
                     /locus_tag="LQB69_000370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL3524561.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38073..38654)
                     /locus_tag="LQB69_000371"
     CDS             complement(38073..38654)
                     /locus_tag="LQB69_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3524562.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(38787..39470)
                     /gene="rpiA"
                     /locus_tag="LQB69_000372"
     CDS             complement(38787..39470)
                     /gene="rpiA"
                     /locus_tag="LQB69_000372"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL3524563.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            39789..40202
                     /gene="rpsL"
                     /locus_tag="LQB69_000373"
     CDS             39789..40202
                     /gene="rpsL"
                     /locus_tag="LQB69_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL3524564.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40286..40756
                     /gene="rpsG"
                     /locus_tag="LQB69_000374"
     CDS             40286..40756
                     /gene="rpsG"
                     /locus_tag="LQB69_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL3524565.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            40850..42937
                     /gene="fusA"
                     /locus_tag="LQB69_000375"
     CDS             40850..42937
                     /gene="fusA"
                     /locus_tag="LQB69_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL3524566.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43109..44296
                     /gene="tuf"
                     /locus_tag="LQB69_000376"
     CDS             43109..44296
                     /gene="tuf"
                     /locus_tag="LQB69_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL3524567.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44703..45011
                     /gene="rpsJ"
                     /locus_tag="LQB69_000377"
     CDS             44703..45011
                     /gene="rpsJ"
                     /locus_tag="LQB69_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL3524568.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45042..45671
                     /gene="rplC"
                     /locus_tag="LQB69_000378"
     CDS             45042..45671
                     /gene="rplC"
                     /locus_tag="LQB69_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL3524569.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45699..46322
                     /gene="rplD"
                     /locus_tag="LQB69_000379"
     CDS             45699..46322
                     /gene="rplD"
                     /locus_tag="LQB69_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL3524570.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46322..46612
                     /gene="rplW"
                     /locus_tag="LQB69_000380"
     CDS             46322..46612
                     /gene="rplW"
                     /locus_tag="LQB69_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL3524571.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46651..47484
                     /gene="rplB"
                     /locus_tag="LQB69_000381"
     CDS             46651..47484
                     /gene="rplB"
                     /locus_tag="LQB69_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL3524572.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47527..47805
                     /gene="rpsS"
                     /locus_tag="LQB69_000382"
     CDS             47527..47805
                     /gene="rpsS"
                     /locus_tag="LQB69_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL3524573.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            47828..48175
                     /gene="rplV"
                     /locus_tag="LQB69_000383"
     CDS             47828..48175
                     /gene="rplV"
                     /locus_tag="LQB69_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL3524574.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48189..48845
                     /gene="rpsC"
                     /locus_tag="LQB69_000384"
     CDS             48189..48845
                     /gene="rpsC"
                     /locus_tag="LQB69_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL3524575.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            48848..49282
                     /gene="rplP"
                     /locus_tag="LQB69_000385"
     CDS             48848..49282
                     /gene="rplP"
                     /locus_tag="LQB69_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL3524576.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49272..49460
                     /gene="rpmC"
                     /locus_tag="LQB69_000386"
     CDS             49272..49460
                     /gene="rpmC"
                     /locus_tag="LQB69_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL3524577.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49485..49751
                     /gene="rpsQ"
                     /locus_tag="LQB69_000387"
     CDS             49485..49751
                     /gene="rpsQ"
                     /locus_tag="LQB69_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL3524578.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            49809..50177
                     /gene="rplN"
                     /locus_tag="LQB69_000388"
     CDS             49809..50177
                     /gene="rplN"
                     /locus_tag="LQB69_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL3524579.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50214..50522
                     /gene="rplX"
                     /locus_tag="LQB69_000389"
     CDS             50214..50522
                     /gene="rplX"
                     /locus_tag="LQB69_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL3524580.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50550..51089
                     /gene="rplE"
                     /locus_tag="LQB69_000390"
     CDS             50550..51089
                     /gene="rplE"
                     /locus_tag="LQB69_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL3524581.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51108..51293
                     /locus_tag="LQB69_000391"
     CDS             51108..51293
                     /locus_tag="LQB69_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL3524582.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51329..51727
                     /gene="rpsH"
                     /locus_tag="LQB69_000392"
     CDS             51329..51727
                     /gene="rpsH"
                     /locus_tag="LQB69_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL3524583.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            51759..52295
                     /gene="rplF"
                     /locus_tag="LQB69_000393"
     CDS             51759..52295
                     /gene="rplF"
                     /locus_tag="LQB69_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL3524584.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTCPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52456..52812
                     /gene="rplR"
                     /locus_tag="LQB69_000394"
     CDS             52456..52812
                     /gene="rplR"
                     /locus_tag="LQB69_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL3524585.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            52833..53333
                     /gene="rpsE"
                     /locus_tag="LQB69_000395"
     CDS             52833..53333
                     /gene="rpsE"
                     /locus_tag="LQB69_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL3524586.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53348..53527
                     /gene="rpmD"
                     /locus_tag="LQB69_000396"
     CDS             53348..53527
                     /gene="rpmD"
                     /locus_tag="LQB69_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL3524587.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53571..54011
                     /gene="rplO"
                     /locus_tag="LQB69_000397"
     CDS             53571..54011
                     /gene="rplO"
                     /locus_tag="LQB69_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL3524588.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54011..55306
                     /gene="secY"
                     /locus_tag="LQB69_000398"
     CDS             54011..55306
                     /gene="secY"
                     /locus_tag="LQB69_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL3524589.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55368..56015
                     /locus_tag="LQB69_000399"
     CDS             55368..56015
                     /locus_tag="LQB69_000399"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL3524590.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56206..56424
                     /gene="infA"
                     /locus_tag="LQB69_000400"
     CDS             56206..56424
                     /gene="infA"
                     /locus_tag="LQB69_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL3524591.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56457..56573
                     /gene="rpmJ"
                     /locus_tag="LQB69_000401"
     CDS             56457..56573
                     /gene="rpmJ"
                     /locus_tag="LQB69_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL3524592.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56591..56956
                     /gene="rpsM"
                     /locus_tag="LQB69_000402"
     CDS             56591..56956
                     /gene="rpsM"
                     /locus_tag="LQB69_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL3524593.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            56984..57373
                     /gene="rpsK"
                     /locus_tag="LQB69_000403"
     CDS             56984..57373
                     /gene="rpsK"
                     /locus_tag="LQB69_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL3524594.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57453..58391
                     /locus_tag="LQB69_000404"
     CDS             57453..58391
                     /locus_tag="LQB69_000404"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL3524595.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58419..58799
                     /gene="rplQ"
                     /locus_tag="LQB69_000405"
     CDS             58419..58799
                     /gene="rplQ"
                     /locus_tag="LQB69_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL3524596.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59071..59910
                     /locus_tag="LQB69_000406"
     CDS             59071..59910
                     /locus_tag="LQB69_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3524597.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            59886..60755
                     /locus_tag="LQB69_000407"
     CDS             59886..60755
                     /locus_tag="LQB69_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3524598.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            60745..61542
                     /locus_tag="LQB69_000408"
     CDS             60745..61542
                     /locus_tag="LQB69_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL3524599.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            61745..62491
                     /gene="truA"
                     /locus_tag="LQB69_000409"
     CDS             61745..62491
                     /gene="truA"
                     /locus_tag="LQB69_000409"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL3524600.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(62824..65493)
                     /locus_tag="LQB69_000410"
     CDS             complement(62824..65493)
                     /locus_tag="LQB69_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL3524601.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(65731..65919)
                     /locus_tag="LQB69_000411"
     CDS             complement(65731..65919)
                     /locus_tag="LQB69_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL3524602.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            66189..66551
                     /locus_tag="LQB69_000412"
     CDS             66189..66551
                     /locus_tag="LQB69_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3524603.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            66603..68285
                     /locus_tag="LQB69_000413"
     CDS             66603..68285
                     /locus_tag="LQB69_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL3524604.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            68402..70438
                     /gene="recG"
                     /locus_tag="LQB69_000414"
     CDS             68402..70438
                     /gene="recG"
                     /locus_tag="LQB69_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL3524605.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            70487..71488
                     /gene="plsX"
                     /locus_tag="LQB69_000415"
     CDS             70487..71488
                     /gene="plsX"
                     /locus_tag="LQB69_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL3524606.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            71610..71852
                     /gene="acpP"
                     /locus_tag="LQB69_000416"
     CDS             71610..71852
                     /gene="acpP"
                     /locus_tag="LQB69_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL3524607.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        23627 a        12774 c        15134 g        20344 t
ORIGIN      
        1 aacctttatg taaatgcatg tcctgggagt gggaaaacaa gggtacttac aaggaaaatt
       61 gcttaccaag caattaaaca cgatggatca ctaaaaagaa taattgctat cacatacact
      121 aatagagctg ctgaagaaat taaggaacgc ctaagtttac ttagtataga tgatgatatt
      181 aatatttggg tggggacaat acatcagttt tgtcttgaat ttattattta tccttttaaa
      241 atgaatctct caagagtatc aaaaggattt acaattattg atgattatac tcaaagagct
      301 tacgtcagcc aaattaataa tatgctgaat ttaaatctca aaacgtatga actgaacaaa
      361 attgatttgt cactttctca tgattttaaa attaatgaag ttcagtatac tcaagtttct
      421 gaaatgtatc acactatgtt ggaaaaaaat aaagaaattg attttgatat gattttacta
      481 gtagcgtata aaattttaac tgtaaacagt aacgttgcac gtaatattgc tagtaatata
      541 agggcgattt atgtggatga atttcaagat actagagaat tacaatacaa tgtaatagct
      601 aaattacttc aaaacaaccc tcaaattcag tcaatgtttg ttggagatat tgaccaagca
      661 atttatggat cattagatgg tatagctaaa tcagttgaag aactagaaat gctaactagt
      721 cacacttttc aatcaaagac gctacatggc tgttacaggt ctaatcagag attagtagac
      781 ttctactcta attttcagag ttgtccttac gagattgaaa gcagagggaa taataaaaac
      841 gaccgtggtt ttataagtta tgattgcaaa gttactaagg aggatttacc caacataata
      901 aaaaatataa taaaggaaaa gattgaaagc ggcattcatg aaaaagatat ttgtgtactt
      961 gctcctcaac actatcctct gttttcactg ggtgagtatt taaagaaaga attaccgtat
     1021 tgcaattttg atgctataaa tatatcacca attaaggtaa ataatcatag tttatttttc
     1081 aagttagcaa ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag aaagctaatt
     1141 gcaaacgata ttttaattat attaaaaaat gaatttggaa tagaagttaa agattatttt
     1201 attgacttag atttattaca gctaataaac agtgtgaaaa gaatgtatag tggagacgac
     1261 aacggtgttg atttatacaa atttgttacc aaacatatat ttgttaatct taatattgac
     1321 gagaaaaatt acccgtctat ttttaagcac tatctatttt ttattaacga agtagaagat
     1381 cggataaaaa ataattcatt atctagtagt gtatctagtt ttaagaagat gtttaaatct
     1441 cgagatggta tcacacttac tacagcacat aaagttaaag gaaatgagta tgatactgtt
     1501 atcgctatta atcttctaaa cgggaaaatt cctcattgga atgaaatttt taatgtttct
     1561 gataaaggaa ttagttcagc gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat
     1621 aatttatttc ttttctcaga ggtgggttat tttacgagta aaggagactt gctttatccc
     1681 actcaattat tagataaaat agaatttgat tataatattt aaaataccta ggtagtcgtt
     1741 gttttaaata tagtaggaat cggacgctca gtacggagcg tctatttttt tacccaattt
     1801 tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct
     1861 ttaaaaccat cacacaaata agattcattc aaacataaaa acagaaagga taggtaaaaa
     1921 tatggaactt aaatatgtca ttcccaatat ggaaaaaaca tttggacaat tagaatatgc
     1981 tggtgaaggg aacattgaac aacgtcgaat aaatggcaga ccaaccattc tctctcgtag
     2041 ttacaattta tactcaacga ttcaacgagc agatgatatt gttgtcattc ttccctccga
     2101 agctggcgag aaacattttg aatccgacga aaaagtcaaa ctcatcaacc cacatattac
     2161 cgcagaaggt tataaaattg gcaatcgagg atttaccaat tacattatgc acgcagacga
     2221 tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga gattagcaga aggtattgtg
     2281 attgaaaaag accagacatt tggcacattg aaattttccg ccttgcgtcg tgaggttcgt
     2341 atccagaatg aagatggtac ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa
     2401 tcaaaaggac aaggacgcat gatacaagtc agtatccccg cttctgttcc tttgaaagaa
     2461 tttgactaca acgcagaagt ggaattaatc aatcctgtgg cagataccgt agcgacggct
     2521 acttttcgag gggcagacgt tgattggtac gtgaaagcag acgatcttgt attaaaagga
     2581 cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg
     2641 cttatgacaa acacaccatt gattttgaaa gaaattccga aggatgaagc catttctttt
     2701 attcggcaat accattattc aaagatactc cctagattgt gtaaatactt tctagggatt
     2761 ttttcagaag aaaaattatt aggcgttgtg gaattaggct ggggtacgca acccctacaa
     2821 accatacgca aactctttcc cgacagttcc cttcaaacca cagattattt agaaattgga
     2881 aagatgtgtt ttcttccaga aatgaatcaa accaattatt ttggcagtca agctctttca
     2941 gctctaatta agtggttgaa agaacatacg gactgtcatt ttttatatac actggcagat
     3001 ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca acttctttta ctgcgggtat
     3061 ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa agatacaccc tcgaagtgct
     3121 cgccttttat tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca ttggctttcc
     3181 caggcatttt gcgaatacaa agggattgag aaaatcaatg gacgcatgtt tcgctatctc
     3241 tatccgttga caaaagaagc gaaaaaactg ttaggacaca ctctctatcg acgtcattat
     3301 tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa atatgaagcc
     3361 atttctcaac caacttttga taaacaggct cgtatctaca atacacaatt attttgatag
     3421 gtggaaagga ggatttttat gccattgctt catgacagag gagagcgaat caaagccaac
     3481 gataagcact tggtctatca ttttgtacta atttcattgc tggtagtttt tctagtcgtc
     3541 ctactccctt ttcattggag agcgttacaa tcggtgaact ggcaagcagt tcagttagat
     3601 acatttttct atcaacttca tacgccatac ttttggagca gtattctcac tgctcttttt
     3661 gtatgcggac ttatcagctg gctttattat cactatcgta tagattctat aaaacagtta
     3721 gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc attggtatga agccgaagaa
     3781 actcaatcag agggattctt taaagattta cccagcaaga aaaccaaaca acgaatccgc
     3841 cattttccta aaatatatta tcgaatgaaa gatggactaa tccatattca tgtagaaatc
     3901 accttaggga aatatcaaga tcagcttttg cacctagaaa acaaactaga aacaggattg
     3961 tattgtgaat tagtggaaaa aacgttgaaa gattcttatg tagagtatgt cctactttat
     4021 gatactattg gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa
     4081 ttgatggaat cggtctattg ggagttcgat tctctccctc acatgttaat tgctgggggc
     4141 actggtagtg gaaaatccta ttttattctc acattgattg aatctctcct gcatacaaat
     4201 gcaaagcttt atattcttga ccctaaaaat gctgatttgg cagacttagg ggcggtcatg
     4261 aataatgtct attaccgtaa agaagatatg ttggcatgta tcaatcaatt ttacgaagat
     4321 atgattgatc gcagtgaaac catgaaacaa caccctaact ataaaacagg agaaaattat
     4381 gcttatcttg gtttgtctgc caattttctt atttttgatg aatatgtggc aatgatggat
     4441 atgctaggtc gtgaaagtac caccgttatt cataaactca aacaaattgt aatgttagga
     4501 cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa atattttagt
     4561 gatgggattc gggacaactt taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc
     4621 tttggtatga tgttcggaag cgatacacaa aaacaatttt tcttaaaacc catcaaggga
     4681 agaggttacg tggatacagg aaaaagtgtg attagtgagt tttatacacc ccttgtacca
     4741 aaaaggtacg atttcttagg agaaatagga aaagtcattc aaaaaaagca atctgagcct
     4801 gtaccgcacg aagtgaaagg tacaggttca gacttgcttt gattggtgtg gcgttagcca
     4861 cgccaatcct ttagccccct ctatctaaca ggggggcaca aatacaactg aaaacaggca
     4921 attctcatca accaatgttg atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact
     4981 caagggtcat agtttacatc ttttttagat tggaggtttg cgaatggaaa acgaaaaaat
     5041 ttggattaag gaattgaaag aaaagcgact ggcttatggc gtttctcaaa ataaactggc
     5101 ggttgcttct catattacca gaccttacct atcggatatt gaaacaggca aggcagtccc
     5161 tactcaacag gtgaaagaag atttactgaa cgctttggaa cgcttcaatc ccgataaccc
     5221 attggagatg ttattcgact atgtacggat tcgattccct accaatgatg tggctcaaat
     5281 tatcggtgag gttttacgtt tgaatatgga ttatatgcta cacgaagact ttggctatta
     5341 ttcttatccc gaacactatc gtttcggtga tattgttgtc ctagtctctc atgatgtaag
     5401 taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa actttctatt
     5461 ggctcaacat cggagctggt atgatttctt tcaagattgc atggaacata aaggtatttt
     5521 caaacgcttg gatttagcta tcaacgataa aacagggatt ttgaatattc cagatttggc
     5581 aaagaaatgt aaacaggaag aatgcatttc cgtctttcga agctttaaaa attatcgcag
     5641 tggtgaattg gttcaccgtg atgaaaaacc atacatggga aacacacttt atattggctc
     5701 attaaaaagt gaagtctatt tctgtatata tgagaaagat tatgagcaat acgtgaaaaa
     5761 tgatgttccc ttagaagacg cagaagtaaa gaatcggttt gaaattcggt taaaaaatga
     5821 ccgagccacc caagccatga aagatttatt ggcacatcaa caggcggaaa aaacagcctt
     5881 tgaaattatc aatcgctata tccgctttgc agataaagac gacactaaac gtcggagtga
     5941 ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag cattacgcct
     6001 aaccacacag ccagagcctt attcttttga acgaacattg aactggttac atcatcaagt
     6061 cgcccctaca ttaaaaatcg caagcatttt agatgttctc aacggtacaa ccattatctc
     6121 caccatgatt caggaagcca aactgacaga aaaacatgag aaattaattg aacaacaaca
     6181 tctagccatg gaggatttga ttacatgagc cttttaacaa aagaattaaa gaagttaggg
     6241 tttcagtgtg gcattgagtt tcaagcgtat atacaaaata cagggaaata tacgtctctc
     6301 attattgagg gaaaacggca agctggtgat accatctata cctatgattt ttacaaggtg
     6361 agattttata ataattatac caatcgagta accgtctatg gagagcattt aacccctttt
     6421 caattattaa ggcgtgtaaa aagctatatc tattatcgag aaaaatatct taaggaaagg
     6481 aggacaatca catgaatttt gggcagaatt tgtacaactg gttcttggat aatgcacagt
     6541 cattggtatt gttagcgatt gttgtcattg gactgttttt aggttttaaa cgagaatttt
     6601 caaaactcat tggcttcttg attattgctc taatcgccgt cggacttgtg ttcaatgcag
     6661 ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat cattggtgct taacagatat
     6721 ttttcatgat aaaataggaa gtatatattt tgttatgaaa ggatttatga atatgtttac
     6781 aaatgatcaa tttaaatctc ttttaattaa aacattagaa agaaagagct cttcaaatta
     6841 ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga
     6901 gactgacgat tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata
     6961 tcttaaaata agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat
     7021 tacccagaca gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat
     7081 tttagtacca cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga
     7141 tgtggtttca caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg
     7201 tgcctttgac agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata
     7261 tgaaattaca gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt
     7321 aattgaaaaa gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc
     7381 atcaaatgga atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc
     7441 taatacaaat attattggaa agagagaagc aaaactaata ataggtattt caagtacagt
     7501 tacagattat attagcggat accttgataa gtagcgtcat taactgattg ctactttttt
     7561 tatgactatt tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa
     7621 tttaggacgg tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga
     7681 ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga
     7741 ttttgagctt ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg
     7801 tgaagccatt caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg
     7861 catgtggttt agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag
     7921 tgattgtgat tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg
     7981 tgaagtccct tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga
     8041 aatagagggt aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta
     8101 acaatccgtg ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg
     8161 tatttgaaaa tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt
     8221 ccttttactt tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag
     8281 aggaaaaagt tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt
     8341 cagatatgct agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa
     8401 gagaatattt tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc
     8461 aaactcttgc tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag
     8521 aagccatgtg tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt
     8581 tttcagttag aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta
     8641 atgacaataa aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca
     8701 atttaagttt cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg
     8761 caaaatggac aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt
     8821 gattgttctt tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta
     8881 caccagtatt tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt
     8941 cccaattacc tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt
     9001 tggcagttta ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc
     9061 cattgttctc actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt
     9121 tctaaagtct atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat
     9181 acgattaaaa aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga
     9241 tggcatatcc gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat
     9301 tcgcttatta tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc
     9361 aagttcatga taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc
     9421 tgcaactagc aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca
     9481 ctggacgatt aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt
     9541 ctatgattgg tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca
     9601 atgaacagga agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat
     9661 tctttcatgg tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa
     9721 tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga
     9781 ttcgtaaatt ggagaaagat gattttggtt atattattga acatctttac gggcaaacag
     9841 gtgtcccttt tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt
     9901 tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct
     9961 atttacggat tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta
    10021 ttgttggtga attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta
    10081 ctttccccgt tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta
    10141 ccgttcgcaa taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca
    10201 acgaaaccag tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt
    10261 tagaccaaac caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga
    10321 atttggacga gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta
    10381 tcaaattagt acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa
    10441 gcaagcgata tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg
    10501 gtttcggtgc tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg
    10561 atacaggcag aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg
    10621 taacaaatgc attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta
    10681 acaatatgct ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa
    10741 aagctgaacg tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg
    10801 ttaatctcac gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac
    10861 tcccgaaaga ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca
    10921 gccgagatag cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca
    10981 aacaacgagg gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca
    11041 atagtattgc tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca
    11101 gtgatggcag tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg
    11161 cagatttggt cttacccgat tccaatagct cttttgaaga atacactacc atggaattat
    11221 taagtgtggc aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc
    11281 gttccatttt taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag
    11341 gaaaagctct atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt
    11401 tcgttactca aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga
    11461 aatttgcctt tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg
    11521 tggacaagga agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt
    11581 tagtttcaga catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt
    11641 tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa
    11701 acaaaagaaa tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact
    11761 tttattaggc acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga
    11821 tacaagcaat gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga
    11881 caatagctgg ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta
    11941 tggattgtat gccattacta attttatttg gacaatttct ctttatctat cgaacgccac
    12001 aggttatttg gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat
    12061 tggcgaaaac atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt
    12121 ttatgtcggt ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg
    12181 attggttaag cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt
    12241 tattctatcg gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt
    12301 ttccaaagat gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac
    12361 ggatagtcaa gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt
    12421 aaagcaacca tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga
    12481 acgtgtggaa agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa
    12541 tgtagtgatt gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat
    12601 ttccagatta ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt
    12661 tttccttcta acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt
    12721 tttgccaatc agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc
    12781 cattaccaag ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt
    12841 tgcctttagt atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat
    12901 tatgttttta caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag
    12961 cctattcact ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc
    13021 tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg
    13081 tggaaaagca gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa
    13141 agctaaccat actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa
    13201 tgtgaaaaag caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt
    13261 ggacacgaaa aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac
    13321 accaacccat gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac
    13381 agactttaaa caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca
    13441 gcaggagcaa aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga
    13501 aaaaagacga aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa
    13561 gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg
    13621 accattgaaa aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct
    13681 aaataaaaga acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt
    13741 agtgtggtct tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat
    13801 gatacggaca gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt
    13861 tcaccagaag tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt
    13921 gaagactatg tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa
    13981 gatgttatgc aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag
    14041 tccatcaaac aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga
    14101 gtggatatag attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg
    14161 aggagtcatg ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg
    14221 ggtggtcaaa aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga
    14281 tataactatg gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc
    14341 ccaacagaat ttgatggtga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa
    14401 gggtttccct atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta
    14461 atacaatggg tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat
    14521 gacgctacgc aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat
    14581 tcaacctata atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc
    14641 ttttatcatg caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac
    14701 catctgattg gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat
    14761 aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca
    14821 aagaaaagtt ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt
    14881 tttattgatt ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca
    14941 tactgtccgt gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag
    15001 ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga
    15061 taaacgaaat gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga
    15121 aatgattcgt aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt
    15181 ttctcaagtg aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga
    15241 agacaaagac aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc
    15301 agataatatg gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta
    15361 ccaacccaaa cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac
    15421 tagcttttta gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta
    15481 ctatgtaagt aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt
    15541 gaatcctatc tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt
    15601 agatcaagaa acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga
    15661 taattggaag attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat
    15721 gtactctttt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat
    15781 ttaagtgact atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt
    15841 caatttgata gcgggaacaa ataattagat gtcctttttt aggagggctt agttttttgt
    15901 acccagttta agaatacctt tatcatgtga ttctaaagta tccagagaat atctgtatgc
    15961 tttgtatacc tatggttatg cataaaaatc ccagtgataa aagtatttat cactgggatt
    16021 tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt aatattggag
    16081 ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta tataacagtg
    16141 gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt
    16201 tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata
    16261 cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt
    16321 cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
    16381 aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca
    16441 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
    16501 tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga
    16561 actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga
    16621 aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa agcataagat
    16681 ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat atagggattg
    16741 ataaccttat agaagttatt actaataaat tttattcatc aacacatcga ggtccgtctg
    16801 aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt cttgcatata
    16861 tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa
    16921 aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag
    16981 cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg
    17041 gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa
    17101 caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa
    17161 tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac
    17221 tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc
    17281 atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg
    17341 cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat
    17401 ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat
    17461 acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag
    17521 gattatatgg ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata
    17581 gccctgttag tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct
    17641 ttagaaaagc tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac
    17701 aggaatatct ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata
    17761 ctcaactgaa aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag
    17821 attatcgcaa tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa
    17881 aaggatatca ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga
    17941 tagataaagt aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa
    18001 atatggtttc ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga
    18061 aaggaggaga ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa
    18121 acaagactac gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa
    18181 tgtaagaacg aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca
    18241 gacgccaaga cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt
    18301 tgtttatctg aattttactg actagccttc aatatttctt gcttcattga taccttttgc
    18361 tagtgcttcc attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg
    18421 taaccttcga gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac
    18481 atggagtaat gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc
    18541 tatatttgtt aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag
    18601 accccgtttc attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc
    18661 ttctttttta cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa
    18721 caggtataga aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat
    18781 taatgcgatg aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt
    18841 tataaaattt agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa
    18901 gtcgccatga gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa
    18961 cttatccacc tgtactggat agatatgacc atcaatgtgg ttttgcagat tacatagata
    19021 gaaaaatcaa agcatatgaa cagagaaaac aatctgttta tgtgcttttt tggtcactta
    19081 ataaatatct tttaggattt ctcttcattt atgcaacaaa tccccctttc gtgtcggggt
    19141 ttagtatgaa gggagataaa gaagcacatg ccactccctt tcacgacaga aagggggtga
    19201 aaagcatgaa accatcttct tttcaagaaa ccatagaaaa tcagtttgac tatatctgca
    19261 aacaagttat tgaagatgaa cgaaaagatt acttcaaaca tctagacaga ctatcagcaa
    19321 aagaagtctc gttttctgat atagataact accttgtcaa ctctttttct actgttgata
    19381 cgtacgtcac tgatttccaa ctatttaaat tatttggatt aagaatcaat gttgaaagtg
    19441 atttattaag tgaagccctg cgtcatcttt cagagaaaaa acggaacatt attttacttc
    19501 attactttat ggatatgagt gatgtggaga ttgcggagtt actaaatttg aatcgttcga
    19561 ctgtttatcg tcatcgaata agtggactag ccatgattaa agaattcatg aaggagtgta
    19621 aagaatgaaa acagcttatc caagggttcc cttccccctc attgtaaagg cgacagatgg
    19681 cgacgtggaa gcaattaatc agattgttaa acattatcga gggtatacct caaaacgttc
    19741 attacgtcgc atgacagacg aatatggaaa ttctcatatg gttattgatg aaaccctacg
    19801 tggcagaatg gaaacgaaac tcatctctaa gattttatcg ttcgaaatta aatagtccca
    19861 ttttttcgtg gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag gtttgttatt
    19921 ccatcaaagc atattgtgtg ttcagtaggt tttgataggc taacaagcca aacgttcttt
    19981 gacaaccaaa taaaggcaat cgaatacgtt tcggtaagaa aagagctgat gaactaacac
    20041 gccatgacct acaaaattag tgagcgattc atgttagtaa ttcgaaagta atctttagca
    20101 ggattgctgc catgactttc ttatcgtgat aatgatactc ccgtatagcc atagtccgag
    20161 cgttagaagc gtcacaggca atgggtacgg ctacatgaga accatgcaga agtgaaattc
    20221 ctgtgagctt tgctagagct gttcgattgc taagatattc ttctcttgtc agtgtattat
    20281 tataaaacta tttgatataa ttataggtga atattggagt tttcctagcc tataattggg
    20341 aaaggagtaa taatgataac ggtcacaaaa aaggatttag ttgagctagg atatggaact
    20401 tctttttcag cagacatcat aaaaaaagca aaagaattga tgattacaaa agggcatatc
    20461 tactatcaat ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga aattctagga
    20521 attcggtttt caaatgaaaa caggtctgag tagtttttgc acagtaagga gtaaaaacta
    20581 tggttaaaga cccaataaaa aaagcaaaga atggaaccta ttattttcgt gcaaatctag
    20641 gttatgattc taaagggaaa aaaattcaaa aatatcgtag tggattcact actaaaaaag
    20701 aagcaagaga agaatattcg aaactgctat tgatgaaacc agaagaatta agtgagaata
    20761 aggataaaat gacgtttaaa cattatatct ttgaaatttt ccttccatgg tataagacac
    20821 aggtaaagct cagaacatat gaaaatcgct taccaactat taagaagcat ttctcttatt
    20881 ttgataaaat ggctgtttcc gatattgagc ctattgatgt gcaaaattgg caactaaaat
    20941 tatcaaagaa gtgtaagtct tcttatgtaa gagctgttca aggactattt tcagttgcta
    21001 tggatcgagc aattgtcctt gggataacca ccacaaatcc atctaagatt attggcaatg
    21061 tgaaaaagca aaagtctaaa attgaatttt ggacaaaaga agaatttgaa aaagtaatct
    21121 ctctaattta caaagaagat tactatcaac actttttatt tatctctctg tggtttttat
    21181 ttatgactgg aatgcgaatt ggtgaagcta ctgccattca atgggaagat attgattttg
    21241 actcaggagt actaaccatc aacaaaacac tctattacaa gaatcaatcg aattatagat
    21301 ttgttgaacc aaaaacaaag gcgagcgttc gtcatattgt attagataaa tgcacattaa
    21361 cttatttatc cgaatggaag gaagtacaac aaaatctaat tcagacagac tttgtaatga
    21421 gctataacgg tattcctacc caaaaacata cactatcata tgctattgaa cgctatgcta
    21481 agctggctgg tatccatcga atcagaatcc atgggttacg gcattctcat gcttccttgt
    21541 taatcagtat gggagaaaat ccacttatca ttaaagatcg tctaggacac gaagatattg
    21601 agacgacttt aggaacgtat ggacatcttt atcctaacag taatttcgag gtagctaaca
    21661 aattaaatgg agccatttcc tttaaagaag caaaacaaaa ttatgatact tcaccaaaga
    21721 atcaatttac tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa taaaagtgca
    21781 gtaaaaagat aagaagagcc agaaaccctt ggtataagag gatttctagc tctcctacaa
    21841 ctattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga tacggttgac
    21901 gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc atgggatacg
    21961 ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga ctgtgtagtc
    22021 gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa cagtgaaata
    22081 ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga tagcatcaga
    22141 ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa taccaagacc
    22201 aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact gagtccctaa
    22261 ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa attgcatatc
    22321 ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt ctgtaccaga
    22381 ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt cttcaccagc
    22441 aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga ttttacgttt
    22501 ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg catcgacttt
    22561 gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg cttcgccttt
    22621 gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg ctttttgtaa
    22681 aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga cttttttgtc
    22741 accaacttgt tcacggattt tttctacttg catatcgata aagttatcca ttgtccagtt
    22801 accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc cgtattcgga
    22861 atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt cttggataga
    22921 agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa ctttagttac
    22981 caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa acaagacagc
    23041 ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag caggctctac
    23101 ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta atacaggaat
    23161 acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata cactgtttgg
    23221 tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg cagtaatgcg
    23281 gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga tcaattggtt
    23341 atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat cggcaacgtt
    23401 tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa actattttaa
    23461 aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt ttatggagaa
    23521 ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc aaattcacat
    23581 ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata gcaaaacgat
    23641 aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa cgatccaaat
    23701 accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca aaaaagtcgc
    23761 tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca acgatcaaga
    23821 tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca tcatgtgagt
    23881 aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa tcgatcagca
    23941 tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc tcttttaata
    24001 tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt cgtttgaagg
    24061 tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg gctacacttc
    24121 cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat aaacctttac
    24181 tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca agaggtacat
    24241 agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata ttatctagtt
    24301 catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct tctcttgcca
    24361 cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca tcaatggaac
    24421 tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg atctttttgt
    24481 ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg gatacataaa
    24541 aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa atcattacta
    24601 ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt taaaaggcag
    24661 aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg attatggttc
    24721 acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa agatcttaga
    24781 tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac agcggtttgt
    24841 tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg caaagcgtaa
    24901 ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc atgaagaaac
    24961 ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg tacatgaaat
    25021 tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg tgatccagaa
    25081 gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta atgcagaaat
    25141 cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac tagctgaaaa
    25201 atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta tcagttcttt
    25261 ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga aatagtagga
    25321 acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat agaaggctgc
    25381 caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt tctctattct
    25441 tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc aataggatga
    25501 agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact gtctcacaga
    25561 ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg ttgtcgatcg
    25621 tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat cgacattcaa
    25681 agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa ctgatcgaag
    25741 gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat tctcgtcata
    25801 gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg gacggaatct
    25861 tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt ttttatcaaa
    25921 aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga cggcttatcg
    25981 taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat ggccatacga
    26041 agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt ttcaacggat
    26101 atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt gaacatggag
    26161 agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat aaagatatca
    26221 cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg atcgatgaat
    26281 tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta aagctgtcag
    26341 aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt ggattttatg
    26401 ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg aacaaagtgt
    26461 tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc taactcggcg
    26521 gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat ttttagtaaa
    26581 ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga atgaagacaa
    26641 ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg gaaacgagct
    26701 gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga ttccagatta
    26761 tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg aagaaatcac
    26821 gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt ctcttgaagg
    26881 aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat tgattttagc
    26941 ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg gtctaggtta
    27001 cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg agctttcgga
    27061 tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc aatcaggaaa
    27121 cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga ccgttgattc
    27181 tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag cagatgcaat
    27241 cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag atgcaagacg
    27301 tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag tggcggttat
    27361 ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag ttgtcgaggc
    27421 aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct acgccttagg
    27481 aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa gaacgttgct
    27541 tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga tccaaaatca
    27601 agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac gctacacaat
    27661 ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg aattaggaat
    27721 tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg atcacgctgt
    27781 tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt cactattgac
    27841 gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata aaaatattga
    27901 aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag aataaaggaa
    27961 aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa accgaatgat
    28021 cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa ttatcgaaga
    28081 agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg cgctagcgaa
    28141 agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc ctctaggagc
    28201 aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg gtgcagatga
    28261 agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac aagtccaaaa
    28321 agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag ttattattga
    28381 aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa aagctgcagg
    28441 cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg tagaggatgt
    28501 ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg gcggtatcca
    28561 taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg gaacaagcgc
    28621 aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc aaaggagaag
    28681 ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt agataaagcc
    28741 tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc aggcaagatt
    28801 tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc cgagcgcaca
    28861 gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt cgtagcgggg
    28921 cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc agaattctgt
    28981 tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc aacgaccgtt
    29041 cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt cattcagtca
    29101 gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg aaaaaagcaa
    29161 aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt gcatgcggca
    29221 acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca actgcagcat
    29281 tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct tgggaaggac
    29341 tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc caatatttcc
    29401 aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat gctggattca
    29461 aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa gcaacaaaca
    29521 atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat aacgttgtat
    29581 ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca gcttatacga
    29641 ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc gatcgttttg
    29701 atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa gaaatcaaag
    29761 tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca attgctcaag
    29821 gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca ggtaacggtg
    29881 tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg gttatcggtg
    29941 ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag aaaaacttca
    30001 cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca caaaaatcag
    30061 cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa gacggtgttg
    30121 gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa gcaaaagaaa
    30181 aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa aaccatttct
    30241 atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa gaattgttca
    30301 tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa gtaatgtttg
    30361 tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc tagatagaaa
    30421 tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat gcagaatatt
    30481 accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt aaaggcagga
    30541 gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat gaacgtgctt
    30601 tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga agtaaagatc
    30661 acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca ctttatgctt
    30721 gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag tcgcgcaggg
    30781 atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca gtatggattg
    30841 tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca acgggtagaa
    30901 attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc gacagctgta
    30961 ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt caaagaaggc
    31021 aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc tgaccgctgt
    31081 acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt aacctcacaa
    31141 caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa aaaagaagcg
    31201 aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa tcgtggatta
    31261 gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg tatcgctgga
    31321 atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg aaaggcagaa
    31381 agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg aaaaatcact
    31441 gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt cctagatatg
    31501 acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag taaaaacggc
    31561 atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga atatgatgtt
    31621 cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca gcaaaaagca
    31681 attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa tccaactcgt
    31741 ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca aagggacaaa
    31801 tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt ttcggatcgt
    31861 attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga aacatctgaa
    31921 aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa agagttagaa
    31981 aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat tagtgcctgt
    32041 actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt ttggttttca
    32101 gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa gagggatagg
    32161 tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt ctgtagctaa
    32221 ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt ggatcgtcag
    32281 tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc cgttatgcgt
    32341 gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac ttcgagcatt
    32401 ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat tatacgtaag
    32461 cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg actcttccaa
    32521 tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt ttggcggctc
    32581 tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt ggctgctgat
    32641 gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt ttgcttctga
    32701 gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt ctggcgcttt
    32761 ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct ttgttcaaac
    32821 gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt ctggatcagc
    32881 aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg gacttggcat
    32941 gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt tgattatctt
    33001 ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc aagggaaaaa
    33061 acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc atgaaggggg
    33121 cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac actggtttat
    33181 tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag cgggatcgta
    33241 aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt tttcaacttg
    33301 acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt gggtggcgtc
    33361 gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg tgctgaccat
    33421 attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt cttgatcaaa
    33481 gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta tttttctttc
    33541 ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac attcttacct
    33601 gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa gacgaaattc
    33661 ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt aggtatcaat
    33721 gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg tatgggcggt
    33781 gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt tggacaaggg
    33841 ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc aatgggtgcc
    33901 tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt accagttatt
    33961 tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat cattgtgctg
    34021 gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta tatcaaatca
    34081 aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg tctcgccctt
    34141 tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt aaacgcgtac
    34201 atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat aagttcggtg
    34261 atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac gttccacatt
    34321 tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca gtgaaagatc
    34381 atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg atgacaggcc
    34441 actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct aatggattcc
    34501 ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta tgtaacaaac
    34561 cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa acaggcgatt
    34621 taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa gaaatcattc
    34681 ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa gatgatccgt
    34741 atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac ttcacacgaa
    34801 caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt gattctttga
    34861 aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc aacggacaag
    34921 gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag ctgctgaaag
    34981 ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt gatgcattat
    35041 acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt gacttacgaa
    35101 ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca gccgatcacg
    35161 gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg ttattggttt
    35221 acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca gatatttcag
    35281 caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc ttcttgaaat
    35341 tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg agatgttaca
    35401 agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg gtttgattct
    35461 aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga tttcttatga
    35521 tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc tagtttatgg
    35581 gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact atgaaggtca
    35641 ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg cccattcagt
    35701 cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag atttgatgct
    35761 gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga atgaagatgc
    35821 aatgggccct agatttccag atatgagtca agcttatacg cctgactatc aagcaacagc
    35881 aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata tgggctattc
    35941 tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag gagccgatgc
    36001 agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat taaaagtgtt
    36061 aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga gccatgaaga
    36121 agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta aagaaacatt
    36181 gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg aaagaaggcg
    36241 agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat attgcggaga
    36301 cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc tttaccggca
    36361 aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca tcagccacca
    36421 tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga gtaggtacat
    36481 gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa ggtgcagcga
    36541 ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca atcagtgatt
    36601 tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg gttcatgtag
    36661 gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg ttccaattag
    36721 ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac ttaggcgcga
    36781 aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc accggtgaag
    36841 aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta ggattggaaa
    36901 cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta ataagaacat
    36961 ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg caaatgtaag
    37021 cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga gtcacaagga
    37081 acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga ccgagaagtt
    37141 ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc acagtccctt
    37201 ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc atttaattca
    37261 taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc agagatacct
    37321 tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa aacagttttg
    37381 ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg ttctaatgtc
    37441 actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta acgacggtca
    37501 tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc gtaagaacga
    37561 cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt gttcaaacct
    37621 tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg tacccaaagt
    37681 tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa aacagaagtg
    37741 taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc ttttgcttct
    37801 tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt caatgcattc
    37861 cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc tccttttaag
    37921 ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt tggaagagat
    37981 ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat aaaaaaacga
    38041 acagtgccca cgcaactgtt cgtttttagg tattagatgc ataattgctc gatttctttt
    38101 tcaggtaaat tgatatattt ataacatgtt ccaactgaaa caccgcattt tgaagaaata
    38161 aattggatcg tttctttttt ttcataatag agacgtcgga tttttttgat cgtttttgca
    38221 tcgatttttg gtcttccgcc gatttttcct tttttacgag cttcatctaa tccaactaat
    38281 gtacgttctt tgatcaatgc gcactccatg tttgctaggt tttccattaa ttggaaatag
    38341 atttttccca tttcgcgcgt atcgattcct tcatcaagac taatcaagtg cagcccgcga
    38401 tctttgaata attgcgtgag ttctgttaat tgccgcgtag atttgcctaa tcgatgaagt
    38461 tggcaaatga ctagtgcatc gccttgttgc atgtcagcga tcactgactc caaatgaaca
    38521 gcagtagaat cattttgttc cacttcatat gtctcatgga aaatttcttg acaaccaaag
    38581 tcagataatt gatcaacctg agttgttaaa tcaacgtcat ttatggttgt gcgtgcataa
    38641 ccaataattt tcatggtgat tcctttctag cagtgatttt ctaactggat ataacagtaa
    38701 aaatgtagca taaagtacgt caaaaagata gttttttcgc aaattttccg ctgtcttttt
    38761 ttattggatg atcaagcagt ttttctttat ttacgacgga ttgcgtcaag aatctcaacg
    38821 cctttttctg agccgacaac tactttagaa accatgttta ggaaaagccc atgttctact
    38881 accccaacaa gttcatctaa ataggtagct aagctttctg gctgttcgat gacttctaga
    38941 tgcagatcaa tgatgtaatg accaccatct gtggttaaga cttctccgtt ttcgtctgta
    39001 cgcaagacgg gttggaatct tttttcttca aaaatatgaa gtagctgttg ggagccataa
    39061 gggatcactt caactgggag tgggaacttc ccaagtttat cgactaattt agaagaatca
    39121 acgatccaaa tcgtttcttt tgagtacgta gcgactattt tctcgaaaag caaagcagcg
    39181 ccgccgcctt tgatcccttg aaaatcagca ctgatttcat cagcaccgtc tattgtcaaa
    39241 tcgactacgg ggacttcatc aatactcttt aacggtattc ctaatttttc cgcttgttct
    39301 tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac cttcttcacg catacggcga
    39361 ccaatctctt ctaccatata ataggcggtt gaaccggtcc cgagacctac gatcatcccg
    39421 tcctttacga atttcgcggc ttcaatgcca gctaattgtt ttaaattcat ctttgcgtcc
    39481 accttttttt agtttgagca tcgtgctttc ctcactattt tagcgtgatt tacggtaaaa
    39541 agataccatt cttttttatt ccaataaagc tttttcaaaa aaaattgcat ctcttgttga
    39601 catgtttttt cattcgtgat attctaacaa aggtgcttta tctacaggtt tctgtgaaga
    39661 aacgtgctga ctgtttatca aagcatattt gataaggatg cagacattgc atttattttt
    39721 tatcacaaaa aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg ggaaggagga
    39781 aacaaggaat gcctacaatt aaccaattag tacgtaaacc tcgtaaatca aaggtggaaa
    39841 aatctaattc acctgctttg aacaaaggat ataacagctt taagaaaact caaactaacg
    39901 taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca cctaaaaaac
    39961 cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt gaagttactg
    40021 cttatatccc agggattggt cataacttac aagaacacag tgttgtatta ttacgcggtg
    40081 gtcgtgtaaa agacttacca ggggtacgtt accatatcgt tcgcggtgct ttagatactg
    40141 ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa cctaaagctt
    40201 aattttaatt attaaacaaa tgaaacttgc ttctactata tagaagtaat tactatgaac
    40261 aatatttcgg aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa aacgtgatgt
    40321 tttaccagat cctatttata actcaaaatt agtaactcgc ttgatcaacc gtgtaatggt
    40381 tgacggaaaa cgcgggattg cagctaacat tatctataat gcctttgact tgatcaaaga
    40441 atctacaggt aacgatccat tagaagtgtt tgaacaagca atgaaaaacg ttcaacctgt
    40501 tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac caagtaccag ttgaagttcg
    40561 ccctgaacgc cgcgttactc taggtcttcg ttgggtagta aactacgctc gcttgcgcgg
    40621 tgaacacaca atggaagaac gcttagcaaa agaaatcatg gatgctgcaa acaacacagg
    40681 tgcttctgtt aaaaaacgtg aagacactca taaaatggct gatgccaacc gtgcgtttgc
    40741 tcactatcgc tggtaagatt ctctctgttt ggtcggcttt tataagtcga cctcagatag
    40801 tgtccttatt tttagcaact agatagtaaa gagaggagaa acaaggagaa tgaccagaga
    40861 attttcttta caaaacactc gtaatatcgg tatcatggcc cacatcgatg ctggtaaaac
    40921 gactgctact gaacgtatct tgtactatac tggtaaaatc cataaaattg gtgaaactca
    40981 tgaaggagct tcacaaatgg actggatgga acaagaacaa gaacgtggta ttacgatcac
    41041 ttctgctgct actacagcac aatggaaggg tcaccgaatc aacatcatcg acacaccagg
    41101 acacgtagac ttcacagttg aagttgaacg ttcattgcgt gtacttgatg gtgctgtaac
    41161 tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac
    41221 aacttatggt gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg gtgctgactt
    41281 cttatattca gtatcaactt tacatgaccg tttgcaagca aacgctcacc caatccaatt
    41341 accaattggt gcggaagacg acttcacagg aattatcgac cttgtaacaa tgaaggctga
    41401 aatgtataca aacgatcttg gaacagagat cgaagaaaca gaaattcctg aagaataccg
    41461 cgaattagct gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa ctgatgaaga
    41521 acttactttg aaatacttag aaggtgaaga aatcactgaa gctgagttaa aagaaggtat
    41581 ccgccgcgca acagttaacg tagaattcta cccagtacta tgtggttcag catttaagaa
    41641 caaaggggtt caattactat tggacgctgt tcttgattac ttgccatcac cgttagatat
    41701 cccagctatc aaagggattg accctaaaac tgatgaagaa gtagaacgtc ctgcagacga
    41761 cagcgctcct ttctcagctt tagcatttaa agttatgact gacccattcg taggtcgttt
    41821 aactttcttc cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac aaaacgcaac
    41881 aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg catgccaact ctcgttcaga
    41941 aatctctgaa gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag acacaactac
    42001 tggggacaca ctatgtgatg aaaagagtct agttattctt gaatccatgg aattcccaga
    42061 accagttatc caagtcgcta ttgaacctaa atcaaaagct gaccaagata aaatgggtgt
    42121 ggctttacaa aaactatctg aagaagatcc tactttccgt gctgaaacaa acgtggaaac
    42181 aggcgaaaca atcatcgctg gtatgggtga acttcactta gatatcatcg ttgaccgtat
    42241 gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct caagtatctt accgtgaaac
    42301 tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg gtaaaggtca
    42361 atacggtcac gtatggattg aatttacacc aaatgaagaa ggtgctggtt tcgaatttga
    42421 aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg aaactggttt
    42481 gaaagatgct atggaaaatg gtgtcttagc tggctatcca ttagtagata tcaaagctaa
    42541 actttacgat ggttcatacc atgatgtcga ttcaaatgaa actgccttcc gtgttgctgc
    42601 ttctatggca ttacgtgcag cagctaagaa agctaatcca gtaatccttg aaccaatcat
    42661 ggctgtagaa gttgttatcc ctgaagatta tttaggtgat gttatgggtc acgtaactgc
    42721 tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag ttgttcgtgc
    42781 gatggttcca cttgctgaaa tgtttggtta tgctacaact cttcgttctg ctactcaagg
    42841 tcgtggtaca tttacaatga catttgacca ctacgaagat gtaccaaaat cagtacaaga
    42901 agaaatcatt aagaaaaacg gcggaaaagc tgaataattt cttaaaaccg gatagtcctg
    42961 caaagagatt atccaaagag actatatttt taatatagtt tcgtgtaaaa taattatcga
    43021 tgatatgggc atgtggtgta ccttgtgtac atcatatacc tatcagacaa ataaaaaata
    43081 tattgtattt ttaggaggac attttaaaat ggcaaaagaa aaatttgacc gttctaaacc
    43141 acacgttaac attggtacta tcggacacgt tgaccatggt aaaactacat taacagctgc
    43201 aatcacaact gtactatcta agaaaaacgg cggccaagct atggcttacg atcaaatcga
    43261 tggtgctcca gaagaacgcg aacgcggaat cacaatctca acagctcacg ttgagtatga
    43321 aacagataca cgccactacg ctcacgttga ctgcccagga cacgcggatt acgttaaaaa
    43381 catgatcact ggtgctgctc aaatggacgg agctatcttg gtagtttctg ctgctgacgg
    43441 cccaatgcct caaactcgtg aacacatcct attgtctcgt caagttggtg ttccttacat
    43501 cgttgtattc ttgaacaaag tagacatggt tgatgacgaa gaattactag aattagttga
    43561 aatggaagtt cgtgacctat taacagaata cgaattccct ggtgacgatg ttcctgtagt
    43621 tgctggatca gctttgaaag ctctagaagg cgacgcttca tacgaagaaa aaattcttga
    43681 attaatggct gcagttgacg aatacatccc aactccagaa cgtgacaacg acaaaccatt
    43741 catgatgcca gttgaagacg tgttctcaat tactggacgt ggtactgttg ctacaggtcg
    43801 tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa gttgttggta ttgctgaaga
    43861 aacttcaaaa acaacagtta ctggtgttga aatgttccgt aaattgttag actacgctga
    43921 agctggagac aacattggtg ctttactacg tggtgttgca cgtgaagaca tccaacgtgg
    43981 acaagtttta gctaaaccag gtacaatcac acctcataca aaattctctg cagaagtata
    44041 cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcactaact accgtccaca
    44101 attctacttc cgtacaactg acgtaacagg tgttgttgaa ttaccagaag gaactgaaat
    44161 ggttatgcct ggcgacaacg taacaatgga agttgaatta atccacccaa tcgctatcga
    44221 aaacggtact aaattctcaa tccgtgaagg cggacgtaca gttggtgccg gtgtcgttac
    44281 agaaatcaaa gcttaattca cttaagtttt aaagtaaagc aacatttatt cggacgtaat
    44341 cgctcggaac gaaggtctta ggatctttgt tccgagcttt tttatttatt gcaggatcag
    44401 gaaaaaagac aggagtatta taaatagaag aaacacgaaa taaaagagtc agtattttct
    44461 aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct ttgtgagttt
    44521 atatatatta taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac gtaatcagaa
    44581 tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc ggacgctttg ccacgaacga
    44641 gcgtgacgga aattttcgtg gagctatgtc tacttttcaa aataggcgaa ggagggaaca
    44701 agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt attttagatc
    44761 aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg tctggtccaa
    44821 ttccattacc aactgaacgc agtctttaca caatcatccg tgcgactcat aaatacaaag
    44881 attcacgcga acaattcgaa atgcgtacac acaaacgtct tatcgacatt gtgaacccaa
    44941 caccaaaaac tgttgatgct ttgatgaagc tagacttacc atctggtgta aacatcgaaa
    45001 tcaaattata aaactaaaac aaaaataatg gaggtgtact catgaccaaa ggaatcttag
    45061 ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg tgaattaatt ccagtaactg
    45121 ttgtagaagc tactccaaac gtagtactac aagtgaaaac aatggaaacg gatggctacg
    45181 aagctattca agttggttac caagatatgc gtgaagtttt atcaaacaaa cctgcgaaag
    45241 gtcatgttgc aaaagcaaac acggctccta agcgcttcat tcgtgaattc aaaaatgttg
    45301 agctaggaga atatgaagta ggaaaagaaa ttacagtaga tgttttccaa gcaggagaca
    45361 ttattgatgt aacaggtact acgaaaggta aaggattcca aggggttatc aaacgccacg
    45421 gacaaagccg cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct gggtcaatgg
    45481 gtccagttgc acctaaccgt gtatttaaaa acaaacgtct tgctggccgt atgggtggca
    45541 accgcgtaac tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga aatgttatct
    45601 tgatcaaagg taatgttcct ggagctaaaa aatctttaat tactatcaaa tcagctgtga
    45661 aagctaaata attgtaggaa gaaaggagga actaaggaat gccgaatgta gcattattca
    45721 aacaagatgg aagccaaaac ggtgaaatca cacttaacga agaaatcttc ggaattgaac
    45781 ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta agacaaggaa
    45841 cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca tggcgtcaaa
    45901 aaggaacagg tcgtgcacgt caaggatcta tccgctcacc acaatggcgt ggaggtggcg
    45961 tagtctttgg accaacacca cgttcttaca gctacaaact tcctaaaaaa gttcgtcgtt
    46021 tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa caacttggta gcaatcgaag
    46081 gcttgaactt cgacgcacca aaaacaaaag aattcaaaca agttcttgct aacttatcta
    46141 tcgactctaa agtattggtt gttctagaaa aaggtaacga ctttgctgct ttatcagcac
    46201 gtaacttacc aaacgtttct gtagtaactt ctgataatgt gagtgtgcta gacgttgtat
    46261 caaatacaaa agtgttagca acacaaactg ctcttactca aattgaggag gtgcttgcat
    46321 aatgaactta ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa tgcttgccat
    46381 ggatgacaag aaatacactt ttgaagtgga cactcgcgca aacaaaactt tagtaaaaca
    46441 agcggtagaa gctgcttttg acgttaaagt caaaaacgtg aacatcgtga acgtacgtgc
    46501 gaaattcaaa cgtatgggca aatatgcagg atatacaaaa aaacgtcgca aagcgatcgt
    46561 tacattgact gaagattcaa aagaaatcca attattcgaa gctgctgaat aagcactaat
    46621 agataactca ttaaattagg agggaaaaac gtggcgatta aaaagtacaa acctacctca
    46681 aatggtcgtc gtaacatgac aggttctgat tttgctgaaa tcacaacatc aacacctgaa
    46741 aaatcattgt tacaaccatt gaaaaaccat gctggacgta acaacaacgg ccgcatcact
    46801 gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg tgatcgactt caaacgtaac
    46861 aaagataacg ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg ttcagctaac
    46921 atcgcgttag tacactatga agatggagtg aaagcttaca tcttagcacc taaaggacta
    46981 gaagtgggta tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg aaatgctctt
    47041 ccattagaaa acattccagt tggtactgtt atccacaaca ttgaaatgaa acctggtaaa
    47101 ggtggacaat tgatccgttc tgctggtaca agtgctcaag tacttggtaa agaaggcaaa
    47161 tacgtattga tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc atgccgtgct
    47221 acaatcggtt ctgtaggtaa cgaacaacac gaattaatca acatcggtaa agctggacgc
    47281 tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc taatgatcac
    47341 ccacacggtg gtggtgaagg taaagctcct atcggacgta aagcaccagt ttctccttgg
    47401 ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga caaacttatt
    47461 gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg tcgcacattt tgagaggagg
    47521 ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg atgagcattt aatgaaaaaa
    47581 gttgaagctc aagcaggggt tgaaaagaaa aaagttatca aaacttggtc acgccgttca
    47641 acagttttcc caagttttgt tggatatacc atcgcagttt atgatggacg taaacatgta
    47701 ccagtttaca tccaagaaga catggtagga cacaaattag gtgaatttgc acctacaaga
    47761 acttatcgtg gacatgctgc tgacgataag aaaacgaaac gctaatttga ggggaggata
    47821 aatccaaatg gcagaacaaa tcacatcagc taaagcaact gcaaaaacag ttcgcacttc
    47881 acctcgaaaa gcccgtttag taattgacct tatcagaggt aaaagtgtcg cagatgcaat
    47941 ttcaatcttg aaattcacac caaacaaatc tgctggaatc attgaaaaag tattgatgtc
    48001 agcagttgcg aatgcagaaa ataactttga cttagatgtt gaaaacttgg tagtaagcga
    48061 agcttttgtt aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa aaggatcagc
    48121 ttctccaatc aacaaacgta caagtcatat cacggtagtc gtatcagaaa aataaggagg
    48181 gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc atccgcgact
    48241 gggatgctaa atggtatgct gaaaaagagt atgctgagtt cttacacgaa gatttaagaa
    48301 tccgtaaatt tatttcaact aaacttgctg atgctgctgt atcaacagtt gaaatcgaac
    48361 gcgcaacagg tcgcgtaaac atttcaattc acacagctaa accaggtatg gttattggta
    48421 aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa caaattaact ggtaaaagaa
    48481 tccatatcaa tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta gtaggtgaag
    48541 gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa caagccatcc
    48601 aacgctcaat gcgtgctggc gctcaaggga tcaaaactca agtatctggt cgtctaaacg
    48661 gagcagatat cgctcgttca gaaggatact cagaaggaac tgttcctctt catactttac
    48721 gtgcggacat tgattacgca tgggaagaag cagatacaac atacggaaaa ctaggagtta
    48781 aagtgtggat ctatcgtgga gaaattcttc caactaaaaa aaacactgag aaaggaggga
    48841 aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg gaaaaatgcg
    48901 cggggaagct aaaggcggaa aagaagtagc attcggtgaa tacggtttgc aagctgttga
    48961 ttcacattgg atcacaaacc gccaaatcga agctgctcgt atcgcaatga ctcgttacat
    49021 gaaacgtggt gggaaagtat ggattaaaat tttccctcac aaatcttata ctgccaaagc
    49081 aattggggta cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg caccagtaaa
    49141 acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc gtgaagcgtt
    49201 acgtctagct tctcacaaat taccaatgaa aactaagatc gtaaaacgtg aggaaatggg
    49261 tggtgaatcg aatgaaggtt aaagaaatca gagaattaac cactgccgaa atgcttgatc
    49321 aagaaaaaca attaaaagaa gaattgttta atcttagatt ccaattagca acaggtcaat
    49381 tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc aaaactgtgt
    49441 tgcgtgaaca agctaagtaa ttatggaagg aggccattaa gcgtatgact gaaggaagaa
    49501 atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa acaattaccg
    49561 ttgctgtcga aacaaagaaa aaccacccta tctatggcaa acgcatgaac tattctaaga
    49621 aatacaaagt gcatgatgaa aacaacacag caaaagttgg cgacatcgtg agaattatgg
    49681 aaactcgtcc attatcagct acaaaacgtt tccgtttgtt ggaagtagtc gaagaagcag
    49741 ttattatcta ataacacgag attttcctat atagattgaa tgcaaaatct gaaaggagga
    49801 tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc tgataactca ggcgcacgtg
    49861 aaattttgac gatcaaagtc cttggtggat ctggtcgtaa aactgctaac atcggtgatg
    49921 tgatcgttgc tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa ggtgacgtcg
    49981 ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg tacagacggt tcatatatta
    50041 aatttgatga aaatgctgca gtgattattc gtgatgataa aagccctcgt ggaacacgta
    50101 tctttgggcc agttgcacgt gaactacgtg aaaacaactt catgaagatc gtttcactag
    50161 caccagaagt tttataatct tgagacactt atcaaaggag gtgcgaaaca gttatgtttg
    50221 ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga caaaaacaaa gaaggcgttg
    50281 tactagcagc gttcccgaaa aaagataaag ttattgtcga aggtgtaaat gtcgttaaaa
    50341 aacaccaaaa accttctcaa gctgctcctc aaggtggaat tgttgagatg gaagcgccaa
    50401 ttcatgtttc taacgtgatg gtggttgact ctactggtgt agctggccga gttggctaca
    50461 aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt ttagataaat
    50521 aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa tatcttaaag
    50581 aagtaactcc atcattgatg gaaaaattta actatagctc tgttatgcaa acacctaaag
    50641 ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct aaaaacttag
    50701 ataaagcagt tgaagaatta actttgatca ctggtcaaaa accaatgatt acaaaagcta
    50761 aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc aattggtgca aaagttacat
    50821 tgcgcggaga aagaatgtac gaattcttag acaaattggt ttctgtttca ctaccacgtg
    50881 tacgtgactt ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac tatactttag
    50941 gtattaaaga acaattgatc ttcccagaag tagattatga tttagtagat aaagtacgtg
    51001 gtatggacat cgttattgta acaacagcaa actctgatga agaatctcgt gagttgttga
    51061 cacaattagg catgccattc caaaaataat taaaggaggc gaactacgtg gctaaaaaat
    51121 caatgattgc taaaaacaaa cgtcctgcaa aattttcaac acaagcttat actcgttgcg
    51181 aacgttgcgg acgtccacat tcagtttatc gtaagttcca tctttgccgt atttgcttcc
    51241 gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa agctagctgg taaaaagtaa
    51301 attcgcataa gaaggaggta aaagatcaat ggtcatgaca gatccaattg cagattttct
    51361 aactcgtatc cgtaacgcaa acatggttaa acatgagtct ttagaagtac ctgcatcaaa
    51421 aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg atgttgaata
    51481 catcgaagat gacaaacaag gcgtgatccg tgttttccta aaatttggta aaaacggcga
    51541 acgtgttatc acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt atgtcaaagc
    51601 tgacgaagta ccaaaagtat tgaacggttt aggtatcgca atcatttcga cttctgaagg
    51661 tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta tcgcttacgt
    51721 atggtaatca agtagaatct acaaggaggt gtctttaagt gagccgtatc ggaaataaag
    51781 tcgttgttat ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt acagttaaag
    51841 gaccaaaagg tgaattgact cgtacttttt cagcagatat caaaatgaat atcgaaggaa
    51901 acgaagtaac tttcacttgt ccaaacgaca gtaaagaaat gaaaacaatt catggaacaa
    51961 ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga aggtttccaa aaagcgttag
    52021 aactaatcgg ggttgggtac cgtgcacaat tacaaggatc aaaacttgta ttgaacgttg
    52081 gttactcaca tccagtagaa atcacaccac cagctggagt aactgttgaa gtaccagcta
    52141 acacacaagt aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta gctgcaaaca
    52201 ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg tattcgttat gttggcgaat
    52261 atgtacgccg taaagaaggt aaaactggta aataatcgtt tagaaaaatt gcatagaagc
    52321 cagcagcagg aaaaaggcat ttggctgaag tttaccttca aaaaaataca tttttacttg
    52381 tgccgcagct tctatttttc gaaacgtatt tactgcttga aatagtagta caataaaaat
    52441 aaagaggtga caattgtgat tacaaaacca gataagaaca aaacacgtca aaagagacat
    52501 cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc cacgcttgaa cgtttttcgt
    52561 tctaacaaaa acatctacgc tcaaattatt gatgacgtag cgggtgtaac gctagcaagt
    52621 gcctctacct tggataaaga aatttcaggt ggaacaaaaa cagaagcagc acaagctgtc
    52681 ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt ctttgaccgt
    52741 ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg cgaaaatgga
    52801 ctagaatttt agaagaagga ggaataccat tcatggttta tatcgatcca aaacatttgg
    52861 aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa ggtggacgtc
    52921 gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta gggtttggta
    52981 caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat gcgaagaaaa
    53041 acttagttga agtacctatg gttggttcaa caatcccaca cgaagtaatc ggtgctttta
    53101 gtggtggtag aatcctaatg aaacctgctg tagaaggttc tggggttgct gctggtggac
    53161 cagttcgtgc cgtattagaa ttagctgggg tagcagatat cacttctaaa tctttaggct
    53221 caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa ttgaaacgtg
    53281 cagaagaagt tgcagaactt cgcggcaaat cagttgaaga attaatcgga taaggaggac
    53341 aacaataatg gctgaattaa aagttacttt aaaacgcagt attatcggac gtcctcaaaa
    53401 ccaacgcgat acagttaaag cgttaggtct tacaaagatt aacagttcag tggttaagcc
    53461 ttctaatgaa gcaattaaag gcatgatcaa tactgtttct cacctagtgg acgttgaaga
    53521 aatttaattt agaaaaatca taactagaac attaaggagg tgccaaacct atgaaacttc
    53581 atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt cgtggtactt
    53641 catctggtaa cggaaaaact gccggacgcg gacaaaaagg acaaaaagct cgttcaggcg
    53701 gcggtgtgcg tttaggattt gaagggggac aaacaccatt gttccgtcgt ttaccaaaac
    53761 gaggattcac aaacgtgaac cgtaaagact atgcagtcat taatcttgat gtattgaacc
    53821 gctttgaaga cggagctgaa gtaactcctg ttgctttagt tgaagctgga atcgtgaaaa
    53881 acgaaaaagc tggaatcaaa gtattagcta atggggaact aaacaagaaa ttaactgtga
    53941 aagcagcaaa attctctaaa gcagctcaag aagcaattga agcggctggt ggatcaatcg
    54001 aggtgatcta atgtttagac tattaaaaga tgcttttaaa gtaaaagaca ttagatcgaa
    54061 aatcctattt actgtatttg tgttgtttgt atttcgccta ggtgcacata ttactgtacc
    54121 aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg ccatttttca acatgttgaa
    54181 cttggtaagt ggtagtgcga tgcaaaactt ttccattttc tcaatggggg tttcaccata
    54241 cattactgct tctatcgtca ttcagcttct tcaaatggac atcgtgcctc gatttgttga
    54301 atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat caagcgactc gatatctgac
    54361 attagtattg gcatttatcc aatctgtcgg tatcactgct ggattcaatc aatacgctca
    54421 attaggtttc gtgaataatc ctaacgtagc aacttacgta acgatcggtt tgattctaac
    54481 agccggtacg atgtttgtta cttggctagg agaacagatc acagaaaaag gtatcggaaa
    54541 tggcgtatca atgatcattt ttgcaggtat catttctaga ttgcctgaaa gtatcagaga
    54601 aatttatgaa gattacttta taaatattga tccttcagac atatggaaat ctgctatttt
    54661 tgtggccatt ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat ttttccaaca
    54721 agctgaacgc aagatcccga tccaatatac gaaacgcgta gctggcgcgc ctacaagcag
    54781 ctatctgccg ttgaaagtaa atgcagctgg agttattcca gttatctttg ctagttcgtt
    54841 tattgctacg ccaaatgctg ttttacaagc attgagcggt agattcggtt ctgaaaactg
    54901 gtacaaagtg atgatggaga tctttaacta taatacagca cctggtgcta cgatttatac
    54961 gattctgatc gttgccttca ctttcttcta cgcctttgtt caagtaaacc ctgagaaatt
    55021 agcggaaaac ttgcaaaagc aaggaagtta tataccaagc gtacgccctg gtaaggggac
    55081 tgaagagtat gtctcaagat tactgatgcg tttaagtaca gttggtgcag tcttccttgg
    55141 tttagtggca ttgcttccaa tcattgctca aatggtttgg gatcttccac aatcaatcgg
    55201 attaggcggt acaagcttac tgatcgttat cggtgtagct ttagaaactg cgaaacagtt
    55261 agaaggctta atgctgaaac gtaaatatgt tggattcatt gagtaagaaa gcgtgttgag
    55321 gtttcccctc aacacacgtt ctcacacatt tattaggagg gaaaacaatg aacctcattt
    55381 taatggggct gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt gatgcttatg
    55441 gtattccgca catctcaaca ggagatatgt tccgcgcagc gatgcaaaac gaaacagctc
    55501 ttggcctgga agcaaagtct tatatggata aaggtgcatt agttcctgat gaagtaacaa
    55561 atggaatcgt gaaagagcga ctagctgaac cagatacaga gaaaggcttc ttattagatg
    55621 gctttccacg tacgctagat caagcaaaag ctctggacgc aatgctaaaa gatttgaata
    55681 aaaaaattga tgctgtcatc gatatccatg ttggtgaaga aatcttggtc gaacgtctag
    55741 ccggccgatt tatctgcagc aattgtggag caacttacca taaaattttc aaccctacaa
    55801 aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga gaagatgata
    55861 aacctgagac ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa ccaatcttgg
    55921 cttattataa agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa atcgatgctg
    55981 tatttgcaga tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag ccgacttgca
    56041 ttatgcagtt actcttgtca agttcaataa attatgatag aatatgtcag tccgacttct
    56101 agagaatcct ctagatgatc cattttgaga attttgaggt gggaactgca gtttccgcca
    56161 ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc aaaagaagat
    56221 atgattgaag tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt taaagttgaa
    56281 ctagaaaacg gacaccaagt tcttgctact gtttcaggta aaattcgtat gcattacatc
    56341 cgtatcttac ctggtgacaa agtaacggtt gaattgtctc catacgatct taaccgtggc
    56401 cgtattactt atcgctttaa ataattgtac tccgtaaaac tacaggaggt attatcatga
    56461 aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt cgtaaaggac
    56521 gcgttatggt gatttgccca gcaaatccaa aacataaaca acgtcaagga taattggagg
    56581 tgctataaat atggctcgta ttgcaggagt agatattcct cgtgataaac gtgtagtagt
    56641 ttcacttact tatatctatg gtattggtaa cactactgct aaaaagatct tagctgatgc
    56701 tggtgtatct gaagatgttc gcgttcgtga attaacgaat gaacaaacag atgctatccg
    56761 tgcagaagta gataaattaa aagttgaagg ggatcttcgt cgtgaagtga acttgaacat
    56821 caaacgtttg atggaaatcg gttcttaccg tggaatccgt catcgccgtg gattgcctgt
    56881 tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc gcgctatcgc
    56941 tggcaagaaa aaataattct taagtgaagg aggttaaaac ttcatggcag caaaaaaagt
    57001 gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt attgcacata tccattctac
    57061 attcaataac actatcatca tgatcactga tactcacgga aatgcattag cgtggtcatc
    57121 agctggttca ttaggtttca aaggaagcaa aaaatcaact ccttttgccg ctcaaatggc
    57181 agcagaaact gctacaaaag cagcaatgga acacggatta aagactgttg aagtaacagt
    57241 taaaggacct ggttctggac gtgaagcagc aattcgttca ttacaagcta caggtttaga
    57301 agtgactgca attcgtgacg tgactccagt tcctcataat ggtgcccgcc ctccaaaacg
    57361 ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc agtaacgtca ttgaacaaga
    57421 cacttttcgt tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa ccaagaatcg
    57481 caaaaattga tgaagaaaaa gattatggca agttcatcgt tgaacctctt gaaagagggt
    57541 atgggactac tttagggaat tccctacgtc gtattttatt atcttctttg ccaggtgctg
    57601 ccatcactaa tattcaaatc gatggtgtac tgcatgaatt ctctaccatc aaaggtgtta
    57661 gagaagacgt cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa atgtatggcc
    57721 aagaagaaaa aacccttgaa atcgatatta ccggacctgc tacagtaact gctggcgata
    57781 ttatcgttga tagtgatgta gaaattctta acaaagatat gtacatctgt agtgtctctg
    57841 aaggagctac gttccatgct cgcttaacag tgaaacctgg acgtggttat gttcaagcag
    57901 atgaaaataa aaaagaagat atgccaattg gcgttcttcc agtcgattca atctacacac
    57961 ctgttcgtcg tgtgaactat caagttgaga acacacgtgt tggacaccgt gaagattttg
    58021 ataaattgac gatggagatt tggacagatg gttcaattga accgttggaa gcaatgagtt
    58081 tgtctgcgaa aatcatgact gaacatttgg atatttttgt gaacttaact gatgaagcaa
    58141 aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg ttggaaatga
    58201 cgatcgaaga gttagacttg tctgttcgct cttacaattg tttgaaacgt gcaggaatca
    58261 atactgtaca agaacttaca aataaatctg agcctgaaat gatcaaagtc cgtaacttag
    58321 gacgtaaatc attagaagaa gttaaagcaa aattgcacga cttaggttta ggtttacgta
    58381 aagaagatta aaacctttta cgaaggaggg aaaccaacgt gagttatcgt aaactaggac
    58441 gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac aactgattta ttaatcaacg
    58501 aacgtattgt gactactgaa gctcgtgcga aagaagttcg ctcaactgca gaaaaaatga
    58561 tcactttagg taaacgcgga gatttgcatg cacgtcgtca agctgcggct ttcgtacgta
    58621 atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca gctttacaaa
    58681 aactgttcaa cgatattgca cctcgttacg cagaacgtca aggcggatac acacgcattt
    58741 tgaagacaga accaagacgc ggtgatggtg caccaatggt tgtcttagaa cttgtctaaa
    58801 atcacactaa taacatcact ttatagatga ttcttttaga gtgttatgat gttggaagat
    58861 atttttccga gtctagctca gcgctgctcc tcaagcgaat atcgcttggg gagtaagcac
    58921 tcatcataaa gtgttttttt ataccttcaa ttacctgtca tgacagatat gtttgaaggc
    58981 ataaaaaaac attttaagcg agaaataatt gaaaattttg tctttttttg atagtgttag
    59041 taggaacttt aggatgaaga aggagtaatc atggagccaa ttattgaatt agaaaagata
    59101 aattataaat accagccaga tgatcttcgc cccgcattga aagatgtctc ctttacaatt
    59161 gataaaggtg agtggatcgc tattattgga cacaatggtt caggaaaatc gacacttgcc
    59221 aaaacgatca atggattgct tctgccagaa tcagggatcg tgaaagtggg aaatcaaata
    59281 ttggatgaag aaaatatttg gacgatccga cagatggttg gaatggtttt tcaaaatcca
    59341 gacaatcagt ttgttggttc gacagtagaa gatgacgttg cttttggtct tgaaaatcaa
    59401 gggattccca gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa agttagaatg
    59461 gctgagtttg cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca acgtgttgcc
    59521 attgccggcg tggttgcctt acgacctgat atcattattt tagatgaagc aaccagtatg
    59581 ctagatccag aaggtcgtga agaagtgatc tctacgatca agaagatcaa agaagaaagt
    59641 caattgaccg ttatatctat cactcatgat attgatgaag cagctaatgc caatcggata
    59701 ctagtgatga gacaaggcga acttgtccgt gaaggtacac caaaagaaat tttttctgct
    59761 ggtcctgaac tgatcgactt aggtctagat ctaccatttc ctgaaaaact gaagagcgca
    59821 ctgaaagaac gtggggttga tgtgcccagt gaatatatga cagaagaaag gatggtggat
    59881 tggttatgga catccgtttt gaacaagtag acttcactta tcagccgaat acaccatttg
    59941 aacaaagagc cttatttgac atcaatatga cgatcaaaga aaacagttat actgcattag
    60001 tagggcatac cggaagcggg aaatcaacct tacttcagca tttgaatgcg cttgttaagc
    60061 caacaagcgg tacagtccat attggagaac gagatattca gccggatacg gataataaga
    60121 atttaaagcc catccgaaaa aaagttggca tcgtctttca gtttccagaa gcacagctat
    60181 tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa aaactttggt gtcagcgaag
    60241 aagaagcact agtcctagca aaagaaacat tagaacaagt tgggctggat gaaagctatt
    60301 tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg tagagtagca atcgctggtg
    60361 tgcttgccat gaggccggaa gtacttgtat tggatgaacc aacggcagga cttgatcccc
    60421 agggcagaaa agaaatgatg gagatgtttt ggcgtcttca taaggaacag cagatcacta
    60481 ttgtattagt tacgcattta atggatgatg tagccaattt tgcagattat gtctatgtgt
    60541 tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa aacattgaat
    60601 ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa gaattaatgg
    60661 ctaaagggat gaactttgct accttgcctt tgacagctga agaattagca gacgctatcg
    60721 tccattatgc tggaggaaat gcccatgatg aataaattga tatttggtcg ttatatgcct
    60781 ggagattcct tgatccatcg attggatccc cgagctaaat tattagccag tttttatttt
    60841 atcggaatca tttttttagc aaataattgg gaaacgtacc ttttgttggc atttttcaca
    60901 ttactttctg tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg cattcgccca
    60961 ttgatctggc taatcctctt cactgtagct ttgcaggtct tgtttacaag tggcggagaa
    61021 acttattgga gttggggtgt tttccatatc actgaatttg gtgtgatgaa cggcctcttt
    61081 attttctgtc gattcgtgtt gatcattttt atgtcgactc tactcacgct tactacaccg
    61141 cctttggaat tatcggatgc gattgagtac ttgcttcgtc cattgaaagc cgtacggttc
    61201 ccagtccacg aaatttcatt gatgctgtcg attgctcttc gttttgtgcc tacattgatg
    61261 gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt tggtgaagga
    61321 aacctagttc agaaaatgaa agcagtcatt cctttgctga ttcctttatt tgtcagcagc
    61381 ttcaaccgtg cggaagatct tgcgactgct atggaagcaa gaggctacaa aggtggggaa
    61441 ggaagaacga aatatcgtgt attgcattgg cataatagag acacgatggt tatcattgct
    61501 tttgtattat taactgtgct actgatcttt ttaagaggat aaacgttcag tgggccgcta
    61561 tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc aagcagtgta tgacaagaaa
    61621 tattagggtc tgtggcagac taaggtcaca ggcctctttc ttttatctga aaagaaagaa
    61681 cgaaaaaatt ttatagaaaa atcattattc cagataacct tttaggaaca aaggagagaa
    61741 aaacatggta cgttacaaag caatcattgc gtacgacgga acaaatttta atggattcca
    61801 aaaacagcca aatgggcgta ctgttcaaga agaagtagaa aaaacattac aaaaaatggc
    61861 aaatggaaaa gaaatcacgg tctttggttc gggaaggaca gatgccggag tacatgcgat
    61921 cgggcaagta atccattttg attatccaga ggagcgtccg ttagaacgaa tgcgtttcgc
    61981 gctagatacc cagtcaccag aggatattgc tgtaagacaa gtagaaattg tttcagaaga
    62041 cttccacgca agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg atatagggaa
    62101 accacgcagt ccttttcgac gccattatgc aagttatttt ccttacccac ttgatttgga
    62161 aaaaattcag cgggcgcttc ctgatttatt agggactcac gatttcacct ctttttgtgc
    62221 atcaggaagc tcgatagagg ataaaatacg tacgatctac gaggctaaga tggaggtaaa
    62281 tgaaacaaaa gacgaattgt tgtttacatt taggggaaat ggcttcttgt ataagatgat
    62341 acggatctta gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg aagatagtat
    62401 cccagatatt attgcgaaaa aagatcgtaa tgcagcagga cctacagcac accctgaagg
    62461 actttatttg tatgaagtga aatatgaata agaggttatg atagaagtgg tcagcttcaa
    62521 gaaataagaa ggtatttacg aaaattactt ctcaaaattt tgcaaaattt cggcttattt
    62581 tcgaagaagc tacttctgga acaccgttta agaaagaggt cgtgagcctg tcatttaact
    62641 ctgagtcaca aagagcaatt tcaaaaaaca gcttctcatc tttgatgaat atcgtgactt
    62701 gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct gtaaccaaaa
    62761 aacgatggaa cgttgttttg attgcccttg gtgcggacag tctgaacagg ttccatcgtt
    62821 tctttatttt ctacgtcttc ttctgtgtgt gtttcgtggt tgttcttcta ttgctggatc
    62881 aatcgtgcca taaaaggggc gattgcggtc aaggtaccag acataaagaa taaacaatgc
    62941 aatacatagg acaaaaagga ttgcacctat cgtaaatcct tcaggcaagt aactgaaagt
    63001 gacagtatgt ttacctgctg gaacttttat catcaagaat gcgtctttga aattgctgac
    63061 cttgactggt ttcccatcaa ttttagcttt ccatcctttg tcataaggaa tagttgtcac
    63121 caacatggaa tcttcagcgg tatttacagt cccagaagct tttctgccag tggcttccat
    63181 ttcgacacct ttttctttga tctgattcat tgcggcttga taggctacaa catctagacc
    63241 aacgacttta ggctccatga aacttatttc tttcgtccca taaaaacttg cagtaaatgt
    63301 cactgttgtc tcttgaggat aatagccaag atcatagtat tgaccagaaa tgttgatttg
    63361 cgtttttcgc gatgtaccat tgacagtgat cgtcgcacta gaactttcta attgcgcaaa
    63421 attggttggg aacaaactta agtaagcttg tgtatttgca ggcacatgga cttcccaagt
    63481 gagctctttt ggctggttat ctgctgtttc tgagtaagtg acgccagtcg ttgtctttgt
    63541 gatcgtcgta ttcaactgat tcttcaaagt aggtgtatag aatgtaaaat actgttcatt
    63601 tagaccagac aatgtattga tcaagtttgt ctgactggtc aaattatctg tcactggctg
    63661 ctcaacatca ttaacggaca acggtgcagt aaatgctagt ggcaatgcat tgacattctg
    63721 atacagtgta tagtcgccgc tttgaccagc ttggcggaag ccatatttac tgaacgtgct
    63781 ttcgttttta gaaatgttat atttgattcc tgtaaaccca tccatcagca aagtattatt
    63841 gttataacgg atattcaaat ttgtccctct tgaacggaaa cctaatttat ccaggtaaga
    63901 ggaagaatta cgatttctta tagaagaaaa caaactgata ccactgtaac catagttgat
    63961 cgaatcgttt gcagatactg gatccagatt ttccaagcgg taaaacgagt cggaatcttt
    64021 ttttgtttca tttacaagag ctttcaagtc aggataagga tcagtatata aactgcggga
    64081 agcataattc caatcatcaa gaatgccatg gatcatactg ttcgtattga ccagtgcttc
    64141 agcagacatg atcaataata acaatatact cagatgattg aatgaaagtt ttttatgctg
    64201 ataaaatccc acagcaaaag cataaatccc taagaaaacc actgtcaata caaaggaagt
    64261 catcgtcaca taggtgtagc ttccttcagg tttgatgccc catgccaccg caaagagagc
    64321 agctacgacc aaaatgattc ctaacaacag cccacgatcg tcttgttcta tcttttccca
    64381 tccttttcct gcaagcagga gcactaagaa agagaacaga taggcataac ggaataaaaa
    64441 catattcggt gcatgcatac catgccagaa caaattcaat ggaacaagat aaaaacttgc
    64501 gataagtatg gcgaaaagac tgccgtacaa aaatttattt ttcaacggta ctttccgact
    64561 ggcaaaataa aacagacaga ggactaatgg tagcagacct gcgtaaacga acggaatcga
    64621 tccgtatttt gtcgtgtcat aaacaccaat catatttttc atgattagat ccagataaga
    64681 agtcgcttct gttttcaacg tagtgatctg actcagtgcc tcgccatttg accgaagatc
    64741 cagcactgct ggcaatacaa tgatcattga agcgccgcct gccagtaaag aagtgatacc
    64801 ataaggaaca atcgtttttt tatattctga ccagtttgtc aatagacggg cgataaaata
    64861 taagaaagaa aatacgccaa tcataaaacc catataaaaa ctggataaaa acaagagtaa
    64921 ataactgata aataaaagtt tcggccgttt aagatccata acacgatgaa tccccaaaat
    64981 cactaatgga agataaacaa atgcatccag ccacatgatg atctccgaat gtgcagtgat
    65041 aaatgagatc aatgcgtaac tgacgctaag tgcaacatgc ccccagcgag gaatcttgaa
    65101 cgtacgtgca agaaaccaaa aagccaatcc ggcacagccg accttaagca aagtcagaac
    65161 atacagtgca tctggcatca tctggtttgg aaaaaagaac accagtggcg tgaagatccc
    65221 gcctagatag taagaaatta aagataaata attcaatcct agagaagcat tccatgtata
    65281 aaaaatactt tgctttccaa gaagaacatt tcggaagctg gcatgaaaat tagaaaactg
    65341 tgaaaaggca tcacttgcca aaatacttct gctgcttcca ggatagattc cgatacttag
    65401 ataaacaagt gctaaaatca aaaacggaat caggaaacta gctgctgcgt agcgtccgtt
    65461 ttctctaaag aatgacttta aatttttttt catatttatt ttccaccaaa tttctatgaa
    65521 gttgtattca gtttatcata tccttgtttt tctctgctta aaaagagtct agttttcaca
    65581 aaaaaataaa atttagaata aataacttca taatttgttc gtataacaaa aataaacgca
    65641 aaaaaagggt aggaatcaaa agtcccaccc ttaaaaattt ttattaaata aaaaaccgtt
    65701 cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc ctgatttcaa
    65761 agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc gaactttttg
    65821 taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt tgtttccact
    65881 ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct cctttgcctt
    65941 gatttggagc aatagaataa ttcagctcat accagactaa catatcatat agaatgggaa
    66001 tttgcaagta cattttcata gatacaattg acaattgaga agaataattg taaattaaac
    66061 aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc cttttatttc
    66121 aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc cgttaaggag
    66181 gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt accaatgatg
    66241 taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta ggaatggcca
    66301 gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat tactcaagag
    66361 gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg attatcagct
    66421 atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa tacaatctag
    66481 aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt gttcgtgtac
    66541 tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg aggattatct
    66601 aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag gcaggtgcga
    66661 atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct gtgccagatg
    66721 gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca gtagtagatt
    66781 caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg ctgatgggtg
    66841 cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct aagcaaattc
    66901 cagatgttgt ctcattaaat gcttctgatc ttgctgcagc cttcactcat ggagttgaaa
    66961 ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta gctagagaag
    67021 cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa gtaatgacag
    67081 ctgttgtaaa aggagcaaag cgtgctttag ctaaaactcc agacttgctt ccagtcctaa
    67141 aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat gagggctttt
    67201 taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct ccagcagaaa
    67261 tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg gcgacagaag
    67321 atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga cctacagtcg
    67381 atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga gattctttac
    67441 ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac ccaggagaag
    67501 tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac aacatgcgct
    67561 tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg aaaaaagaaa
    67621 taccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc caagagctgt
    67681 ttgaaagttt aggcgctaat tatgttatca gtggtggaca aacaatgaat ccaagtacgg
    67741 aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc ttgccaaaca
    67801 ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc ccagtagcag
    67861 ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc aatgatcaag
    67921 cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt gtgagcggtc
    67981 aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa gaaggagact
    68041 ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca gcaacagcgt
    68101 taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc ttggcaggtg
    68161 atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa aaacatcctg
    68221 atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta ttttctgctg
    68281 aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa ggccttcggt
    68341 tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taaaggaggg aggaaaaatt
    68401 tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa aaagagcagc
    68461 tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt atccttttcg
    68521 ctacgaagat attcaggaaa gaaatctaaa cgagatccag gatcaagaaa aggttacttt
    68581 gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa aaagccgatt
    68641 gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta atcaacctta
    68701 tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat gggatgcaaa
    68761 gcgtaaagcc ttgaacggga tgaagatttt aggttctcaa acaatggatg acttttcgcc
    68821 gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa tcaaaaaagg
    68881 gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg tggagaaata
    68941 tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag accacgaaga
    69001 aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc aaatgcgtct
    69061 tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc tttatgatgt
    69121 ccaaaaattg aaacaattta ctcaaaagct accttttgaa ctgacaaatg cacaaaaacg
    69181 tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc gacttttgca
    69241 gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg ctaccgttac
    69301 agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac aacatatgga
    69361 aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgatta cggggtcaac
    69421 gaaagcaaaa gaacgacgtg agctccttga aaaactagag caaggggaaa ttaacgtcgt
    69481 gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag gtctagttat
    69541 tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg aaaaaggctg
    69601 gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag ctattacagc
    69661 atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta tcccgatcga
    69721 aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga aaaaagaact
    69781 ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag aagcattaga
    69841 tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac caaaatatga
    69901 agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca tggaagcctt
    69961 caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag gggtaaacgt
    70021 gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg cacagctgca
    70081 ccaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct tagttgctaa
    70141 tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca ccaatggttt
    70201 tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg gcttccgtca
    70261 atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg ttttggaggt
    70321 agccagagaa gaggctgcag ccctttggca aataaaggat tggcagatgc ttcctgagta
    70381 tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt tcgattagca
    70441 gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga aaattgccgt
    70501 agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg ctttagcgca
    70561 gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa tcaagaaata
    70621 cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca atagtgatga
    70681 tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg ctgcacaagc
    70741 agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg cgctactagc
    70801 agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct tgatgtctac
    70861 cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg gcgctaacgc
    70921 agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt atgcacgtaa
    70981 agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag aagcaacaaa
    71041 aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa gcttgaattt
    71101 tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag tggtcacaga
    71161 tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga atatgatgaa
    71221 tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg ctatgctttt
    71281 aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg gcggtgccgt
    71341 tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc ccgatgctgt
    71401 tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg gacaattagt
    71461 cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcgatc cgttccattg
    71521 aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt aggtaattga
    71581 caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt aacaaagtag
    71641 cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat gaattgaaca
    71701 tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta gagcttgaag
    71761 atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca gtcggtggcg
    71821 cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag aaggataaa
//
LOCUS       DAFFFQ010000005        64195 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000005
VERSION     DAFFFQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.005"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(137..1528)
                     /locus_tag="LQB69_000417"
     CDS             complement(137..1528)
                     /locus_tag="LQB69_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBL3524608.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="LQB69_000418"
     CDS             complement(1741..2127)
                     /locus_tag="LQB69_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL3524609.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="LQB69_000419"
     CDS             complement(2183..2806)
                     /locus_tag="LQB69_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL3524610.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="LQB69_000420"
     CDS             complement(2870..3538)
                     /locus_tag="LQB69_000420"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL3524611.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="LQB69_000421"
     CDS             complement(3550..3990)
                     /locus_tag="LQB69_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524612.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4720)
                     /locus_tag="LQB69_000422"
                     /pseudo
     CDS             complement(4049..4720)
                     /locus_tag="LQB69_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4905..5303)
                     /locus_tag="LQB69_000423"
     CDS             complement(4905..5303)
                     /locus_tag="LQB69_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL3524613.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5300..6073)
                     /locus_tag="LQB69_000424"
     CDS             complement(5300..6073)
                     /locus_tag="LQB69_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL3524614.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6070..6753)
                     /locus_tag="LQB69_000425"
     CDS             complement(6070..6753)
                     /locus_tag="LQB69_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL3524615.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6920..7807)
                     /locus_tag="LQB69_000426"
     CDS             complement(6920..7807)
                     /locus_tag="LQB69_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3524616.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7804..8673)
                     /locus_tag="LQB69_000427"
     CDS             complement(7804..8673)
                     /locus_tag="LQB69_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524617.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8810..10096)
                     /locus_tag="LQB69_000428"
     CDS             complement(8810..10096)
                     /locus_tag="LQB69_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3524618.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10369..10947
                     /locus_tag="LQB69_000429"
     CDS             10369..10947
                     /locus_tag="LQB69_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3524619.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10947..11876
                     /locus_tag="LQB69_000430"
     CDS             10947..11876
                     /locus_tag="LQB69_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3524620.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11873..13609
                     /locus_tag="LQB69_000431"
     CDS             11873..13609
                     /locus_tag="LQB69_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL3524621.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LQB69_000432"
     CDS             complement(13672..14508)
                     /gene="pheA"
                     /locus_tag="LQB69_000432"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL3524622.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14505..15014)
                     /locus_tag="LQB69_000433"
     CDS             complement(14505..15014)
                     /locus_tag="LQB69_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL3524623.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LQB69_000434"
     CDS             complement(15007..16302)
                     /gene="aroA"
                     /locus_tag="LQB69_000434"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL3524624.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16315..17406)
                     /locus_tag="LQB69_000435"
     CDS             complement(16315..17406)
                     /locus_tag="LQB69_000435"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL3524625.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LQB69_000436"
     CDS             complement(17423..18589)
                     /gene="aroC"
                     /locus_tag="LQB69_000436"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL3524626.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LQB69_000437"
     CDS             complement(18591..19655)
                     /gene="aroB"
                     /locus_tag="LQB69_000437"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL3524627.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LQB69_000438"
     CDS             complement(19688..20710)
                     /gene="aroF"
                     /locus_tag="LQB69_000438"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL3524628.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21369..22343)
                     /locus_tag="LQB69_000439"
     CDS             complement(21369..22343)
                     /locus_tag="LQB69_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL3524629.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22324..24207)
                     /locus_tag="LQB69_000440"
     CDS             complement(22324..24207)
                     /locus_tag="LQB69_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL3524630.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24225..26450)
                     /locus_tag="LQB69_000441"
     CDS             complement(24225..26450)
                     /locus_tag="LQB69_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL3524631.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26565..27599
                     /locus_tag="LQB69_000442"
     CDS             26565..27599
                     /locus_tag="LQB69_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524632.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27666..28679)
                     /locus_tag="LQB69_000443"
     CDS             complement(27666..28679)
                     /locus_tag="LQB69_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3524633.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28757..28945)
                     /locus_tag="LQB69_000444"
     CDS             complement(28757..28945)
                     /locus_tag="LQB69_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL3524634.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29385..29513)
                     /locus_tag="LQB69_000445"
     CDS             complement(29385..29513)
                     /locus_tag="LQB69_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL3524635.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LQB69_000446"
     CDS             complement(29516..30889)
                     /gene="rlmD"
                     /locus_tag="LQB69_000446"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL3524636.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31000..32055)
                     /locus_tag="LQB69_000447"
     CDS             complement(31000..32055)
                     /locus_tag="LQB69_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL3524637.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LQB69_000448"
     CDS             complement(32065..33495)
                     /gene="gatB"
                     /locus_tag="LQB69_000448"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL3524638.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LQB69_000449"
     CDS             complement(33492..34964)
                     /gene="gatA"
                     /locus_tag="LQB69_000449"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL3524639.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LQB69_000450"
     CDS             complement(34964..35269)
                     /gene="gatC"
                     /locus_tag="LQB69_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL3524640.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LQB69_000451"
     CDS             complement(35375..37411)
                     /gene="ligA"
                     /locus_tag="LQB69_000451"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL3524641.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LQB69_000452"
     CDS             complement(37428..39662)
                     /gene="pcrA"
                     /locus_tag="LQB69_000452"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL3524642.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39817..41376
                     /locus_tag="LQB69_000453"
     CDS             39817..41376
                     /locus_tag="LQB69_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL3524643.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41487..43418)
                     /locus_tag="LQB69_000454"
     CDS             complement(41487..43418)
                     /locus_tag="LQB69_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3524644.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LQB69_000455"
     CDS             complement(43436..44350)
                     /gene="pfkB"
                     /locus_tag="LQB69_000455"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL3524645.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44347..45099)
                     /locus_tag="LQB69_000456"
     CDS             complement(44347..45099)
                     /locus_tag="LQB69_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3524646.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45258..45467)
                     /locus_tag="LQB69_000457"
     CDS             complement(45258..45467)
                     /locus_tag="LQB69_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524647.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45478..46806)
                     /locus_tag="LQB69_000458"
     CDS             complement(45478..46806)
                     /locus_tag="LQB69_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524648.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            46942..47097
                     /locus_tag="LQB69_000459"
     CDS             46942..47097
                     /locus_tag="LQB69_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524649.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(47171..47758)
                     /locus_tag="LQB69_000460"
     CDS             complement(47171..47758)
                     /locus_tag="LQB69_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL3524650.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="LQB69_000461"
     CDS             complement(47841..49091)
                     /gene="clpX"
                     /locus_tag="LQB69_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL3524651.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49338..50627)
                     /locus_tag="LQB69_000462"
     CDS             complement(49338..50627)
                     /locus_tag="LQB69_000462"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL3524652.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50781..51713)
                     /locus_tag="LQB69_000463"
     CDS             complement(50781..51713)
                     /locus_tag="LQB69_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524653.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51870..52529)
                     /locus_tag="LQB69_000464"
     CDS             complement(51870..52529)
                     /locus_tag="LQB69_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL3524654.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="LQB69_000465"
     CDS             complement(52532..53557)
                     /gene="sppA"
                     /locus_tag="LQB69_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL3524655.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53570..53860)
                     /locus_tag="LQB69_000466"
     CDS             complement(53570..53860)
                     /locus_tag="LQB69_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524656.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54269..56074
                     /gene="glmS"
                     /locus_tag="LQB69_000467"
     CDS             54269..56074
                     /gene="glmS"
                     /locus_tag="LQB69_000467"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL3524657.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56146..57501)
                     /locus_tag="LQB69_000468"
     CDS             complement(56146..57501)
                     /locus_tag="LQB69_000468"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL3524658.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57525..58694)
                     /locus_tag="LQB69_000469"
     CDS             complement(57525..58694)
                     /locus_tag="LQB69_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524659.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58691..59578)
                     /locus_tag="LQB69_000470"
     CDS             complement(58691..59578)
                     /locus_tag="LQB69_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL3524660.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="LQB69_000471"
     CDS             complement(59767..63459)
                     /gene="nifJ"
                     /locus_tag="LQB69_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL3524661.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63658..64179
                     /locus_tag="LQB69_000472"
     CDS             63658..64179
                     /locus_tag="LQB69_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL3524662.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17834 a        13922 c        11463 g        20976 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgtttttta ctgatcactt
     4321 tttaaaagct ttttagaatc ttttgctgac gtctaaaaat agaaaaagaa ttttcgtcat
     4381 ctatttgata acgcatttgt ttttgcagtt ctgctgcagt cagatggatc ttcccatcgt
     4441 ttgtatcgat cattaaacct tctggaaaca gttcaaccac tgctcgatcc caagattcaa
     4501 gagaaagctc gatataatct tttactggta attccaattc taccttaaaa aagtgcaaaa
     4561 cagccgacag cccttctgct tgatagattt ctcctaatgt tagcccctct agatggaact
     4621 ctggtgaaaa atgcagcaat gttttatttt cctgcaattg aacatataga aatgtagatc
     4681 gctcaatcag agtatctttt aaaacaacta aaaaattcat ttttctcccc cttttttcat
     4741 catttttaaa atagcatatt ttgaaaagaa agtctgatgc ttagagtaga atttcataaa
     4801 aatttactat ttcataagta ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct
     4861 catccgcctt cttgtttgtg tttatttcct cttcaaacgc ttgtctatga ttcagacttt
     4921 tctctatcgt tattttcatc taaaagttct tgcattccga taatttctcg atattgatga
     4981 atcttagtga taccaatgac tttgtctaca tcaatactaa aacaaatacc attacctgga
     5041 atatcgatgt tgatgccttg accaatcgca tccattactt tattcgtcaa actatcgggt
     5101 actaatgtca acacaatatc tttttctggc gaaatgtcca aacctaaaaa tttccctgaa
     5161 tcatgtacac cagaacctct tccatgtaag atcgttccgc ctgctgcacc tgcttctttt
     5221 gaaaagtcga taacatcttc gcccattcct ccgtcaacga tcgtcacaat catgtgaaga
     5281 ttcaatgcta caagatcttt catttgcttt cccccaattt ttgcaatctt tctttttcga
     5341 ttctgtcttt ttcatctttt cgtttataaa tatatcctag tactaaagtg gataatattg
     5401 gtgtcaatgc aatcattgca accacaccaa acccgtcaat gatcggatct cttccttcaa
     5461 tttgtgcagc gattgccacg ctcattgata aaataaatgt cgaacacatc gggcccgttg
     5521 ctactccacc gttatcaaaa gccattgcta aaaagatctt gtccactttt cgtcctaaaa
     5581 taaatgctag cccgtatcct gggatcaaga aataataaag agagaatccg acgagcaaac
     5641 gccacataga caatcctact gctgcgccga tcccaattga aattaccatc agcatgattt
     5701 tcgatcggat tctcccctcg ctcaattctt caacctgttt gatcattaca tgaatcgccg
     5761 gctcagcaaa ccctactaag aaccccatta aaaagccaag gggaaccagt atataattgt
     5821 aaggcaatcc cgcaatagca ctgcctaaat gctgtcctac cggtacataa gccatattga
     5881 ctccgtgcag gaaaaggatc aacccaatag tcgttatcaa gaaacctttc ataatgtcaa
     5941 agaaacgctt tttgcgcata cgaaaactaa tgatattcat taaaacaaat aggattacga
     6001 taggtgccac tgccaaaatg acttccatgg taacgatttc gattccatct atcacttgct
     6061 gccagaaact cattggaaga tcacccccaa aatcatcacg gcaagaatcg gccctaacga
     6121 agcaataccg accattccaa aactgtcatt ttcatttttc ttttgtttga tcatactagt
     6181 catccctccg gcaagggcca agataaatgg aacagttact ggaccagtag tcactccccc
     6241 cgaatcaaaa gcaatcggca taaattcgga actggtgaaa aatgaggcga tcaaaacgcc
     6301 tatgtagcct atagccatca attgataata ggatagcttg aatacgaccc gaagcagcgc
     6361 aaaggctaga aatactccag tgccgacgct gacgatccca atcagcaaca ctctgccaat
     6421 ctctccgtca gatatatcat atacttgttg tcccaaaact tgcacggaag gttcagcaat
     6481 cgtgatcacg atcccgatag caaatcccaa actgaccaaa acggtcaaac ttttcttttt
     6541 gatcatatat ttccctacta gatctccgac ttccatcata gaataatctg ctccaaacaa
     6601 aaagagcgtc attccgatca tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc
     6661 tccttctaac ggtgcaaaaa caaacgttaa aatgacaatc aggacagtca tcggcaatat
     6721 agcagcaatc acttctttaa aattttttag catgtatcct cctcctttac tctttaagtt
     6781 taccttaaaa tgaaagagtt ttcgagcaca aacttaaaac gttatcattt aatttttctc
     6841 aacataaaat aacacacgtc ccaaaagtat ctttttactt ggaaaaatga ctttgaagga
     6901 acgcgcttat tgtgtttttt tattttacag agccgatcat tccttgaacg aattgctttt
     6961 gcaaaacaaa aaatacgatg gcagttggta aagtagcgat gactaatcca gccatgatca
     7021 ttccatagtc tggtgtatag gaagcaccca tagcagataa aacaagcgga accgtccgtt
     7081 tatcaggaga ttgcaaagct actaagggcc ataaataatt gttccagcta ctcatgaatg
     7141 tgatgattgc agccgcggca taggtatttt tagccgtcgg catatacact ctaaaaaaaa
     7201 tacttagttc ttttagaccg tctaatcgag cggcttctac tagatctttt ggaaaagatt
     7261 taacattttg tcgaaagaag aagatcaaaa aagcagtgct aacagatggc aggatgacaa
     7321 ccaagaaact gttgattccc aaaaccgttc cgttcaattg tgaaaacatt cgatataaag
     7381 ggatcatcaa tgccgcaaaa ggaaccatca tcgatagcag tagaatattg aaaattaggt
     7441 cttttcgttt actttgataa atctcaaatc catatcctgc cattgacgaa atcagcaatg
     7501 caaacacagt ggtcaccaaa gcgatcatcg ctgaattcca aagggaacga gtaaaattca
     7561 gctcattgga aaacaaattt tgaaaattga tgatcaagtt actgccaatc tttatttttc
     7621 ccgatgtgat atccacgggt gtattcgtca ttcccaaaac catccagaca aaagggaaaa
     7681 tggaaataaa tgataggatg cttaatgctg tatactttaa tactgaccca attgtcgaac
     7741 gaagttctaa tgcttgtttt ttgacgttgg ggcgttcgat tgtttctttt tctaccatat
     7801 ttttcatcga ctttctcctc ctactttcgt ttggattatc gacaatatca caataaagga
     7861 aacaataaca aaggaaactg ctgccgcata tccaaaatta ggcgtgaatt tataactcaa
     7921 attatagatg tattgagata gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt
     7981 ttgtacttca tcaaaaagct gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat
     8041 gattggtttt aaatttggga tcgtcacttt gataaactgc tgccatttag aagcaccgtc
     8101 aatagatgct gcttcataaa tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat
     8161 catattgtat ccagtccaac gccaagtgat cgaaagaatg atcagaactc tcgcccaaat
     8221 tggatgagtc aaccagccaa tcggtgtgtc aatcatacca atgcccaaca acaagttgtt
     8281 tacaaggcca gattgagaaa atagactttt catgatcaga gaataactga ccatcgaagt
     8341 gatacatgga agaaagatag cggttcgaaa caacccttta tatttcaact ttttatcatt
     8401 taaaaggttg gaaataaaaa gtgctagaaa caacatgatc ggtacttgta caagtaaata
     8461 caggaatgta ttaaaaaaag cttttttaaa agttgcatct gtcaacatcc gctgataatt
     8521 acctaacccg ttaaatgtca tattgacagg gggaccactt tgaaaagacg tgatcaaagc
     8581 caaaaccatc ggaataaaaa ccatgattga tatcaaaatg actggcaaga ttaaaaaagc
     8641 atgcccgttt ttgaataaat ctttctgctt cattcttttc cctccaattc tatcattgat
     8701 tatctacatg aaaagacaag aaagacaata tctgtaaggc agacgtgatc ttttttgtct
     8761 tttcgtttgt ctgatgaaaa agcgaagaaa ttcacttttt taccaccgat caattagcca
     8821 attgtgcttc tacttgtttt tgtgtatctg ctagtacctt gtctgtctct tctccattga
     8881 tgattcgatg aagagcttca gctacgactg cttcaattgc ataggtctca tgtccgtaat
     8941 tcacttctgg tatttcagat gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt
     9001 aaaattcaga agcttcttga taagcttctt gttcattcgc gctaagcatc gtagatacta
     9061 gtccgatttc tttcgcaaga gtgcccatca gttcttcatt cgaagcaaac gttgcttcta
     9121 ggaaatcttt cgctgaatct tctccagcta ctcctttgat cacataccag ccagcgccgc
     9181 caaggttaga agcttgcgct ttttgcagat tttctggaag tgcaggtatt ggtgcaatct
     9241 tccattttcc agattggttt tctgctcctt ggatcgttga agaataccaa gcgccagttg
     9301 ggctagaagc tactgctcct gattgtatcg cagttactcc agcattccag tcagaggttt
     9361 gttcgactag atcttctttc aaaagagtgg caaacaattc aagaccatac tttaaagatg
     9421 cattgtcttg gatcgtgact gtctccccat cttcagatgt ataccattct cctgcctgtt
     9481 gcataatcat ccttactcga ccaaggtcag aaggattgac ttcagcgatt ttctttcccg
     9541 tcttttcttt gacatctcgc gctacttgaa tatactcgtc ccacttcaga ttctccatat
     9601 cctcttctgt ataacctgct tctgccatca gatccgtacg atagaagttt gctgttacgc
     9661 ccgaatcgaa aggaacaccg tatattttat ctcctacttt gtttaccgca aatttatacg
     9721 gtgcaaaatg atcttccttt acaatagcag acaaattagc gaatgcatct ggataagaag
     9781 tcaaatagcc ttgtatccta taatcttcaa tcaagacgat attgggcaat ccttctgtat
     9841 tcccgctcgc taaagcggtg ttcagtttct gaacgatatc atcctgagac atcgtaacaa
     9901 cttctatctc tgtttcttcg ttctcataaa ctttttttgc ttcattcact gctttgatat
     9961 tgaatgtctc atcccacgcc cagacagtga ctttttcttc tttgtctgct gtactactag
    10021 cacttccgca tcctgttaag aaacctgatg ctgccgctaa acaaacgact ccagcaatta
    10081 actttctgat gttcattttt atccgctcct tttccatcca ttagttgttt acgtttgcta
    10141 cattcatcat accaaaattt gaaaacggtt tctttattat tcagatactt caaaaatttc
    10201 tcctaaaata ataaaaaaat acaaatcaac tatgacaaaa ggaataatct tagcatctag
    10261 accaaagatc gaaatggtga ggtttcttta aaataaaaat atccttgcat tttctcctag
    10321 aaataagtat ttttaaatta caagactatt cttaaggcgg gtaaaaaaat gccaatacgc
    10381 gtaattctta tcgatgatga acagacgatt ttagatggaa tgaaacagtt gatcaattgg
    10441 tctgagcacg ggtttcaatt aatagggact ttttccagtc cgctgaaagc gcttgatttt
    10501 tctttggtac atgcaatcga tatcgtggtc acagatgtaa tgatgcctga attaaatgga
    10561 atcgaattat cgcggctatt aaaacagcaa caacctgaaa cacagatcct tatcctctct
    10621 agctatgatg aatttgatat ggttaaagat tcttttaaag aaggcgtcgc tgattatctt
    10681 ttaaagcctc gattgagtcc tgattttttt ctaaattcct taactgccgt ttcacaaaaa
    10741 atcaaaaaaa agcccaacaa aagcccaatc accaatcgtt atgagcagat ctgcgagaat
    10801 ctttcacagc agattgctgg acaaactcgt ccattgcttg accctcttct ttttgaacac
    10861 accttgtttt tctgttatat accgaaaaac ggccattcag ttcaaaacaa ggctctaaac
    10921 aaaaacagtc gatcacccat tcattgatgg atagcgacga ttcgtttact atttatcctg
    10981 tttctacctt ttcggaagaa tccggctatg taatcaatac tgattccaaa gatacactta
    11041 ggaagtttgt tcaaaagttt cgtcaagaag tttttccagg tgattttttt gttgtttctg
    11101 attcttttgc aatccaagat atcaatgaaa tattccaaga gatcagaaat tgcaccaaag
    11161 gacaacgttt ctatcataag ggaaagaacc ttgcttatca gcatgaactt cttccgttat
    11221 taaccaacca cgagcagcaa gcaagtagtt atttaacaaa aatcgttcat aaagactacg
    11281 ctttagctgt caaagaactt gagaatttct tcacgacgat attggaacag cctattgatc
    11341 cagcttattt gaaacatgag gcaatcaatc gtttctatgc attactcaac gctttggctg
    11401 acgagcatgt tgggaatgaa gaactttctt tgctcaagat cgcccttcct tctttattag
    11461 ccgatgctga gtatttcgaa gatttttcta ttcttgtcaa agaaacactt gagaaactca
    11521 cccacttttc tgcaaatgca aaaaaagaca atttcgatat cttgactttg atctacgagt
    11581 tcgtccagga aaattatgag aaagaaatca atttacaact tttatccgaa aaatatcatt
    11641 tttcatatag ctatctctca gccatcttta ctgaaaaatt tggtatcaac ttttcaaaat
    11701 atctaaaaaa agtccgtatc agtaaagcca agctcttttt gacggaaaca cggcttactt
    11761 tgacggaaat ctgtgagaaa gtcggctata ccgaactggg ttatttctcc agagttttta
    11821 aagaagagac aggaatgaca ccttctcaat accgtaaagg aacttttgta aaatgagaaa
    11881 aaaaatcaaa gagcatgaac tctttgccaa acttctgttc attttgttca tcggtctttt
    11941 tctgcaagcc atcgtcattt ctttgtttat ttatcatcga tcaagggatg cctatatcca
    12001 gctgttcaat caatcgaatg atgtggtact aaaaaagatt caatctgaat ttgaaagttt
    12061 gaatgataca atcgagaata cattagccgc ttttgattct aatccagctg tcaaaagcta
    12121 tttttccaat gatccagccc aacatatgga acagcttcag caactaagaa caatccaaaa
    12181 aatgaatgac tctctttcaa aaatccatcc aatgatcgat tacgatgtat tgatctttgg
    12241 agaaaatgga agaacttttg tcggaaacga catgctcact gccgtatctg ccgattcttt
    12301 ttttcaatct gcgattgctc agagagtgaa tgaacgtgct gctgatacac aaatgctttt
    12361 tgcacaccac ggattgactc ttcgcgataa gaaagcaccc agcgtcttct ttgtcaaaaa
    12421 actgaaaaat acattgaatc acgtttacgg atatgctgtt ttatcgatct catccaagca
    12481 actagcgaat ctttttcaat ctgtagtaaa tcctgagata tcgaagatct ccttcatgaa
    12541 cgaagaacag acgatcatcg catcaaatga agaaaataca attgggagcc gatcaatgat
    12601 tttcaaaaaa ctaacagtag gtgaaacaac gatttcaaat ggacagcagt tgacccgtct
    12661 tcctctgtac cgccaggatt gtacattact cagtcaagtc gatcttcatt ctttatctaa
    12721 ccaaatgggc gtgatttttc cgattatttt gtataatatc gtgacacttg cttttgctgg
    12781 ctcattggta tttatgtatt tggatcgaca cacaaaatcg atctaccgct tgattgattc
    12841 gctaaaacgg ctagaggaag atggcttaaa agctactgtt cctgtacaag gaagccatga
    12901 agtccaattg ctagggaata cgatcaatca attgctaagt aatgctcagc atcattatga
    12961 acagacgatc caagacgaaa agaagaaacg ttcgcttgaa atccaaacta tgcaagcaca
    13021 gatacagccc cattttattt ataatacgct gactagcttg aaatttttga tttggcagaa
    13081 aaaaacagat gaagcagttc aaggaatcga cagtttcatt actttactaa gaagtacgat
    13141 tgggaaaaaa gaagaagtga ttccagtcaa ggaagaaatc aaaagcgtac aaagttacat
    13201 cgatcttttg agtatgcgat atggcgaaag aatctcggct aaaataatga ttcctgatga
    13261 actgcagtca ttatctatgc cgaatatgat ccttcaacca attatcgaaa acgcctatct
    13321 tcatgctttt cacaataaaa cgcaaggatt catcacggtg tacggcaaaa caaaaaatca
    13381 gcttctttta ttcgaagtga ttgataacgg cgatggcttc gatactgaaa ataccaaaca
    13441 aaaaaatgat catttttcag gaatcggcat cagccatgta gatgagcgga tacgcctaat
    13501 gtatggcgat gcatatggct taacgatcca atcagtgatt gggacaggaa cgactgtaac
    13561 gatttgtcta ccgcttcttc ctaaaaatca atcagaatat caaaaataaa gaatagccaa
    13621 gcgctgattt ttttatttta agttaaataa atcaatcagt attcttgact ttcatctacg
    13681 cgtctcttct actgtcagca ctggataaca gcccaatatt tgactttctc ctccgagcat
    13741 cttgatttct tggagcgcat tctctactaa tgtcattgga cggtccaaca acaaatcgat
    13801 cacgaaaaaa tattctccca agctagtttt caatggtcgg gattcaatct tacttaagtt
    13861 gatttcccgc cagccaaaag ctgccagcat tttatgaagc atccctggcc gattagcagg
    13921 tagtgtcaag atgacactca tttttactgg agctgactgc tggctggtca tctttcggtc
    13981 accgacgatc caaaaacgtg tctggttcaa ctcattatct tggatattct cagctaagat
    14041 ctccaatcca acatgctgtg ctgtttctaa tgaagcaatc gctgctgcgt cttctttcgg
    14101 atgttctgct acatacattg ctgcagcagt tgttgattct gttgctacta aaggaacatt
    14161 aggataatgc gtttctaaaa actgttgtga ttgtgctagt gcttgtggat gtgaaaggat
    14221 ttttgtaatc tttgttgctg gattgcctaa aagctgctgc ttgataggaa gaacgatttc
    14281 acttttaact tcgacttctg gatgtttgga aagaagatcg attgtgtgat gaacagctcc
    14341 ttccaatgaa ttctctacag gaacgacagc cagatcgact tgtttgcgaa acaaagcgcg
    14401 caaacaaaca gggatggaag catacgcagt caattgttct gtaggaaata gctgacaagc
    14461 cgcctgataa gtgaatgaac tttctggccc tagatagctg acgttcatat gcttttgatc
    14521 ctttctataa tggtatcgat gatcttttct ggtgtttgat tggtcgtgtt gattcgatgt
    14581 gtcgcacttt cttcatacca agaaagtcgt tgtgccaata atgcttttat ttcttctctt
    14641 gtttttccaa gagctaaagg tcgaatagac gtttcatctt gttggatacg atcaactaga
    14701 cagtccgctt ctgcctctaa gtaaagaaca atcggctgtg tttttaaaaa acgccgattt
    14761 tctgcacttt ggacaatccc tccaccagtc gcgataattt tgtcttcatt tgacagcttt
    14821 cttaaaatat ctgactccac ttttcgaaag cttttttcac cgtaatagtg aaaatattcg
    14881 gcaatcgaca attggatatg ttcctcaatc tttgtatcta aatctacata ctctttttgt
    14941 aaacattgag ctaacccttt accgattgtt gtttttccag cgcccatgaa accaatcagc
    15001 aaaatgctag ccatgagctt ctcctcctga cacaagttct gctaaatcgt caaaaaatgc
    15061 cggataagag attgaaacag cttcagctcc ttctagttcg caaggtccat cagctatcaa
    15121 agcagctact tgcagcatca taccgattcg atgatcgcct ctgctggaca cttttgcgcc
    15181 atgcagaggt gtcggcccat ggatgatcag accgtctgct gtcgctttta tctttgctcc
    15241 cattttctgt aattcttctg ccaccgcatc aattctgttg gtttctttta cttttaattc
    15301 ttctgcgtct ttgatgatcg tgattccttc tgcttgagta gccactaaag ctagaatggg
    15361 caattcatca atcagacgcg gaatgatttc tccaccaatc gttgctttct tcaaatgact
    15421 cgtcttgaca cttaaatcag ccgattggtt ctgttcattg tgattcgtct gggtaatcgc
    15481 tgcgcccatc tcctccaaca catctaagat accagttctt gttggattga ccccgacatt
    15541 ttttaacaac agttggcttt ccggaacaag aagtcctgct gctagaaaga aagcagctga
    15601 tgaaatatca cctggaacag ttatctgttg accagttaat ttttgcggtc cggtcactat
    15661 gatcgtttta tcttccactg tgatccttcc gccaaattga cgaatcattt cttccgtatg
    15721 gttacgggaa cgttcttttt caacgattac ggatgtgcct tctgcttgta atgccgcaaa
    15781 cagcaaagca gatttgactt gcgcactggc tacaggcata ttgtatcgga taggtgataa
    15841 agagcgggtt cctgtcacag tgattggtag atactggtca ttttcttctc cttgcaagtc
    15901 agcgcccatc tcgcgtaaag gttccatcac tcgccccatt ggacgtttag acaacgaagc
    15961 atccccgaac agagtagtcg taaaaggctg tccagccaaa atacccatca ataaacgtat
    16021 ggtcgtaccg gagtttccca tgtcaattga ttcggttggt tggacaaatg agtggctacc
    16081 gcgaccatga atcttgatca cctcttcctc ttcttcgatc tcgacaccta attgttggaa
    16141 ggcatgtaaa gtactcagac aatcttctgc gcgcaaaaaa ttttgtatag tcgtcgttcc
    16201 ttcagcaatt gctccaaaca tgatgctgcg atgggaaatt gatttatctg ctggtatcct
    16261 aacagtccct cttaatccat gtatttgttg taataattgc atttatccag cctcctattt
    16321 gatccagcaa tgataactgg tattagacat aatgattctt tttgcttgta acaaatcctg
    16381 ttggttttta aaactgattt gcagtattcc taaaatatct tccctggtct cttggatctt
    16441 caaattaatc aaagaaactc ccgcttttcc gatcagtcca gttacttctg caataacgcc
    16501 aggttcatct ggcacatcga taaataaatc aaaaaaggca ggaatcgcgc cttgcttatg
    16561 gacaggcaac tggtctcgac tttcttttgc ccgattgaaa aaagaaaaga tttccgattg
    16621 gttctcagtc atgatccatt ctgctatttg tgtcatttct tgttgccaat ctgccaatcg
    16681 ttccaataat atctgcttat tgctcaacaa aatatctgtc cacatgacag gatctgagga
    16741 agcgatccta gtgatatctc gaaagcctcc tgctgccagt tgtttggctc gcggatactc
    16801 ttgattgaat tgttctgcct gattgactaa gccagctgcc agaatatgtg gaaaatgact
    16861 tagcattcct gttatacggt catgttctct tgctgtaaga gttacatatt ttgctttggt
    16921 tccgcaaaat agttcttgca gtttttcaac atcttttttc gtcttttcca aaggtgtaaa
    16981 gatgtaatag gcattttcaa atagtttagc atctgctgcc tgtatacctg atttatgaga
    17041 accagccatt ggatgtccac cgacaaaccg aagatttttc tgctcagcgg atgtcacgac
    17101 ttgctgcttc gtactgctga catccgtaat gatgacgtga tcagacaagt tgattctttc
    17161 aaggacatca agataagcta aggttgtacg gacgggaacg gctaataaga taacgtctgc
    17221 ttgttctgca cctgtttcaa aatcacttgc aacgtaatca acgatctgct gttctactgc
    17281 ttgctcacgg gtttttgact gactatccca accaactact atcagttctg gattggtctg
    17341 acgaatacaa agtgccatag aagcaccaat cagtccaagc cccacgataa aaacatgttg
    17401 tgccatctta tttgccccct ttttagtaat gtttcaagta gtcacggtac tccttcactg
    17461 cagctttcaa ctcaacaagc gaatcacttg ggaattttga caatagctct gtcgctacca
    17521 cagtggctac aacattttcc gcaacgacgc tagctgcagg aactgctgta ctatccgaac
    17581 gttctacact tgctttgtaa ggttcttttg tatcgatatc aacactttga agtggcttgt
    17641 acaatgtagg aatgggtttc atgaccccgc gaatgacaag tggttcgcca ttggtcattc
    17701 cgccttcgaa gccgcctaga ttattggtac gtcggaaata gcctctttct tcttcccata
    17761 ggatctcatc catcacttct gagcctggta atgaaccaga agtgaatccg accccaaatt
    17821 ctacgccttt aaaggcattg atactaacca cagcttgtgc gattttggca tctagcttcg
    17881 tgtcccattg tacgtagctt cctaatccag ctggaacgcc tccaacgagc acttctacaa
    17941 ttcctccgat cgtatctcca tttttctttg tctgatcgat caaatctttt attctttgct
    18001 caactgctgg atcaacaact cgtacttctg acgcttcaga aatttctttg atttcttgaa
    18061 tcgagatatt tccaggtatt tctgcttgga ttccaccaag acaaatcacg tgtcctgcta
    18121 cgatgatgtc aagttcttcc agaattttct tggcaattgc gccgattgct acacgcatgg
    18181 tcgtctctct tgcagaggac cgttcaagca cattccgaag atctcgatga tggtatttca
    18241 taccgcctac caaatccgcg tgtcctggac gcggacgtgc aactcgccgc agtgattggt
    18301 ctttttcagg cacttctgct acagacatga cagttgtcca atttttccaa tctttatttt
    18361 ctaccactaa agtgacaggt gaaccaattg ttttgccgtg acggatgcca gacgtgatcc
    18421 gaacacgatc tttttcgatc ttcatccttc ctcctctgcc gtaaccagct tgtctgcgag
    18481 cgagttcgtg attgatttct tcttctgaca gaggtaatcc tgctggtagt ccttcaataa
    18541 tggctgttaa ttctggtcca tgggattctc ccgcagtcat ataacgcatc ttacagtcct
    18601 cctaagtaat ctttgaatgt ctctgtggga acaggaacga tttttgcttg cccaatcttt
    18661 tctagcaaga tgatattcaa ttggcttcct cttgctttct tatcatgggt tatcgcgtgg
    18721 aataattcat ctggatgact tagatcgtac gtaatcggca gatggaattt ttgaagtatc
    18781 ctttctaaag cagctgtcga tccagttgga gacaagccga ttttttctgc atgagaagtg
    18841 atggctacca ttccaatagc tactccttct ccatgactga ctaccccata tcctgctgta
    18901 ttttcaattg catggccaat tgtatgacca aaatttaaaa taagtcgatt tccttggtca
    18961 aagacatctt cttccactac ttttcgtttg atttccaacg tagcagtaat gattggaaca
    19021 gcatgttcca gaagatcttt ttcgtctttc agctcgttca gctgtttcca aagcgttgca
    19081 tctgctatag ctgctgattt gatgatttca gcgattcctt ctcgtaaacg acgtaccggc
    19141 aaagttttta gaacctcagg atcaatcaat acaccagctg gctggctaaa tgttccgaca
    19201 aggtttttcg ctttgtttgt attgactgct gtcttcccgc caatactaga atctacttgt
    19261 gcaagtagcg tagtcggaat ttgaacgaaa tggatcccac gcatgaacgt cgaggccacg
    19321 aatgctgcta gatcacccac cattcctcca ccaagtgcaa gtatcccatc gctgcgggta
    19381 aaatcattat ctgctaaaaa atcgtataac tcagctgctt gttctaaaga tttactggtc
    19441 tcacccgccg gtacgattga aaggactggt tcaaaattag cttgtctcaa ttggaacatg
    19501 attttttctg catatagtgg agcaacgttt gtatcagtga tcacagctac tttttgaggt
    19561 ctccaaatcg tttgaagcca tgatccagcc tgttctagtg cgcctttttc aatatgaata
    19621 tcgtattttt ggttatcaac atgtacagta agcattcgtt tcacttcttt cttttaaaaa
    19681 gctggaatta atcgagtgtc tgaatctttt ccattgcttc tttcatgatc gcaacttctt
    19741 tcatcatgcg tgagtaagtc ttttcattca acgattgggg accgtctgac caagcatttg
    19801 caggatcagg atggatctct acgatcatgc catctgctcc gcttgccaca cccgcacgag
    19861 ccattggtgg tacaagttcc caaattcctg taccatgact tggatcaaca atcacaggga
    19921 aatgagttaa ttttttcatc aatgggacag cacttaaatc aaacgtatta cgtgtcgctg
    19981 tttcgtaagt ccgaatccca cgttcgataa aaatgacagg agatttgtct tcgactgcaa
    20041 tgtattctgc agcattaagc cattcattga tcgtaccgga aattccacgt ttcaatccaa
    20101 caggtttacc tgttttaccg acagcagata gcagtttaaa attttgcata ttacgtgcgc
    20161 caatctgtag gatatcgctg tactcagcca ccatatcgat atgcccttca tccatcactt
    20221 cagtaatcac tttcattcca aattcatcag ccgcttgacg gatgtatttc aatccttctt
    20281 cttctaaacc ttggaaagca tatggagaag tacgtggttt gaaggcacca cctcgcagaa
    20341 tttttgcgcc cccggctttg gccattcgcg cacattctct gatttggtct aatccttcga
    20401 tagaacaagg accagccatc gtcacgaaac tgccgtcacc aatttttact ccgtctacat
    20461 ccacgactgt attgccagga tggaattctc gagaagtcaa tttgtatgtg tttaaaatac
    20521 gcacagcatt ttctactcct tcatagcgtg taaatgctcc ttcttgtaaa cttcgcgtat
    20581 caccttttag tccaatcact actcgttctg ttccttgatc gattgagact ttgaatcctt
    20641 ctttttcgat atgatcgacg acttttttta tttgttcttc cgttgcttct tttttcataa
    20701 ttactatcat tgtttttccc gctttctttt gtttacttat ctatcttggc tgattttctc
    20761 tactttttca aggtggtttt acattaattc ctactaaatg accgtgtcct gttgttctca
    20821 ttttcgctcc ttctttccta cttatgaccc agataatcaa caaaaaaaga tgtccctgta
    20881 ggaaattgcc tctacagtga acatcttcaa cgagccttaa aaagcgctgc aaaaatataa
    20941 aatagccact atagatgaat ttctctacgt ggctgtaagt cgtgtttaaa aatctttagc
    21001 catcatagat acaaacctgg tttgtttgta cccacgaagt catgaataca aacctatcta
    21061 aaatagctaa agtaatacgt attcagtttt gtaagttttc tagatttcat aaaaaatcgc
    21121 tccaaactga tgtaagaatt gtacctagta tagacataaa aaaatcgact gtcaatcatt
    21181 tttatgaaac tttttttaat tttctgatag ttaactttgt ttttcagaaa aataacaatt
    21241 ggttttattg aagaaaatag tcatgaacaa aagtatctga gaactttttt actctcttgt
    21301 tcatgacata tagaataaag cagctttgtc ttgtaaactc cgtttttcaa ataataatct
    21361 aaagattgct actgaataat aaagccgatt tcatggtcct cttcgctgct gattcggtaa
    21421 ggtgactcaa catccgctcc ccagctattg atacccccga ctcctcttac ttttgcaaaa
    21481 atcgtaagaa ccgttcgtct ttctggcggt agttcttcta tatgtgttgc ctgttccagt
    21541 tcttctgctt tgaatggtaa agcggaaaaa gcaaatggcg tatcctgctg ttcaaatcgc
    21601 aacgaaaaat cttcttgatc tttatccgta ttatccaacg tttgatttct tgttagttct
    21661 acccatttcg tttccatatg catgccgcat tcttggggaa caaggtatgg ggaaacgggg
    21721 agtcctgcca ctttataaat accttcgaca ccaccagcca tccgatccgg atatgtttcc
    21781 cctgataggc cttcatactc aaaaaatttt gctttcgttg gcataatgaa ccttaaaccc
    21841 aatgcgggta attccggtaa tcctttttgt ccataataat gcatattgac aagcatttta
    21901 ccctctccag cgatttggta ggaaattgtc acttgggttt ttggtactgt gttgatttcc
    21961 agtgtaaaaa tgatttcaac cgtttcagcg tattcattac ttgaatattg attatttact
    22021 ggagcaatcg gcaaatcgat ctttttattg tccaccagta cttctatttc tttgactttt
    22081 ggatacaatc cggctcctaa ccacataccg gatttaaatc caaatccgct acctctgtca
    22141 ttatcggtcg ttgctcgcca aaaaactgga agtgtttccc ggtatagcca ttcttttccg
    22201 tttttattga gcgattccag tccgcctctt gtataagaaa aaatataatg cgtattcccc
    22261 gatttgacac ctaatgtaca gtcgccgaag acgattgcaa ctttttccgt atccatatga
    22321 cttttattta ctgtattttt cataataata tcttacctcc tgcatggcgg gtttttcttg
    22381 tccatctgca aaaacgatcc cattccctga gaattcatag tcacttggtt tatcgtcaaa
    22441 gtcaccgcca tagcgcatca cttctctgcc tgtgattgga tcagtcactt ggatcgcctg
    22501 atctttaaaa tcccatatat agcctccttg gtacatttca taacgatcaa gcaacgtcat
    22561 gtaagaatcc attccaccta acgaattccc catgtcatgc atatattcac ataagatgaa
    22621 cggtttttcc gctttatttt caaggtagtc aatgatctct tctggtggcg catacatgca
    22681 gctttttaca tctgaaatcg aatcttcgaa tgctggattt ggaaaattcc cttcataatg
    22741 gacaagtcga tcggggtcta cttttttgaa gtaatcgttc atttgtcgaa tgttttcttc
    22801 cgcataagat tcatttccaa gagaccaaaa aagaatactt ggatggtttt ttaatgtttc
    22861 gaaattcgtt tttgctcgat caagaacagc cgcctgccac tgaggtactg cacctggtac
    22921 gttgtaagaa ggctcaaatg cgcccatttt ttgccatgag ccatgggatt cgaggttcgt
    22981 ttccgccatc acatggattc ctgcttggtc gcataaataa taccaagtga tctggttggg
    23041 gtaatgagat gtccgtacgc cgttgatctt tgcatttttt atgatagcca aatctttttc
    23101 catatcttct atagtgatgg cgcgtccttt tttcggatgc cactcatgtc gatttacccc
    23161 gttgataatc atccgttttc cattgaagta gatgatttta tctttgattt cgattttacg
    23221 aaaaccgaag gaatagtcaa tatactccgt tactcttttt tcttcgtcta tcgtctccaa
    23281 ttcaagctta tataaattgg gctgaaaatg cccccacagt gccacatttt caaatccatc
    23341 gatgcgaatc gtcatttgct cttttaacgg atattgctgt tgatagattt ccttcccatc
    23401 aggatctagt atcgctacat tcagctgact gtttttggct acttgctcaa aagacaaatc
    23461 tacttcaagt ttgccatttt gacatttgtc atctaagcga ggtttgatcc aaagatcttc
    23521 taaatggttt tgtcctcgag caactaagaa gacatctctg aagatcccaa aaaatcggaa
    23581 aaagtcctga tcttccaaga atgcagctgt acttcttttg taaacctcca cagctagaat
    23641 attttcttct tccaaataag gtgttaaatc aaattcagac ggagtgaacg aatcttcggc
    23701 atacccgata aaatgaccat tcaaccaaat aaacattgct tgttctacac cagcaaaatg
    23761 aatcgagatt tgttttccct ttagaccttc ttctaatcga aaagtcgtca aatacgaacc
    23821 aaccggattg tctttagcag ctgaaaagat cccttccgcc tctttctcag ggcttgtcgt
    23881 gaaaggggga cggcgaaata catgtccctc ccacggatac atcgtattga tgtaatggat
    23941 cttgtcataa tttgctaatt caatatgact aggaacagtg atttcatcaa aagaacaggc
    24001 atcaaaatct tttttataaa atgtttttgg tcgtaccgaa ggatttggcg aataacaaaa
    24061 acgccatgat ccatttaaag actgtttaag ggaggatgtg gtttctactt cttcccttgt
    24121 tgcataacaa acatgatcgc tatgtgcatt tgattggttc actctaaaaa cttgtggatc
    24181 atctaaccat tttatatttg ctttcatact tttttccttc tctctcataa ctttttgaaa
    24241 tgaagcagtt ctgtttggaa atcaccttga aaatcgggga agtaaagtcc aactgtcatc
    24301 aattcggccc ccgaaaatat ccgcttcgag gtctgttcct gataaagtgc ctcatcatct
    24361 aaccccatca gatatacttc atggaacgct ggttgagctg aagctagtat cctccctaaa
    24421 aacacaatag cttcatcttg tttaggagac acaaacatcc atgcggcaat attttcttca
    24481 aaaggactcg ccaaacgata ataatggcca tactggatca ccggacgaat cgtttgatag
    24541 gatataattt ggtcttttac ttgcttttct tcttcaggag acagctttgt caaatccagt
    24601 tcataaccaa aaacagcgct catagctgtt tctcctcttg ttttcaacga agtagatctg
    24661 tcggtctgat gatttggtac agctgataca tgagctgtga tggacgaaat cggataagca
    24721 aggcttgttc catattggat tttcatacgc tccactgcat ccgtgttatc acttgtccat
    24781 gactgcggca tataataaat aaacccggca tcaaaccgtc cacctccacc agaacagcct
    24841 tcccataaga tttccggata atcagttgtc aaatgttcaa gtagttcata aagcccgagc
    24901 atataacgat gcgctacttc tccttgtctc tgtgggtcaa gggtaatcga atagacatct
    24961 gaaagcgaac ggttcatatc ccatttgatg tagtcaagag gaatcgtatc taaaatcttt
    25021 ctcatttgtt tctcaatcgt ctgccgaaca tctaaccttg taaaatccag tacatgttgt
    25081 gatctggaag cagaaggcat tctacctggc tcctgcataa gaaaatcagg atgtgttcga
    25141 tacagttcag aatcaaccga gatcatttca ggttcaaacc ataaaccaaa tttcaatcct
    25201 ttgctatgag cataatctgc tatttcacgt aatccatttg tcaacttgcc ttcatactcg
    25261 aaccagtctc ctaatgaaga attgtccgca tctctttttc caaaccagcc atcatctaat
    25321 acgaacattt cgatgcctag ctcctttgct tcatcaatga tcgccttgat tttttcacta
    25381 ttgaagtcaa agtaagtggc ttcccagtta ttgaccagaa tcggccgttc agcaaattga
    25441 tgacgacctc ttgcgattct ttcacgtagc aactcatggt ggatctgact cattttgttc
    25501 aatccctgac ttgagtaaga aaggatcatt tcaggtgttt gaaaagactg gcctgcattc
    25561 aattcccacg aaaaacgatg gctgttgatc ccaccgacga ctctcaattg attgatctga
    25621 tctttttcaa gttcgaaaga atggttgccg gaataaacaa attgaagtcc gatcgcttct
    25681 ccagtatttt ctgtcgtatg atgatgaact aaagcaataa agttattcat atggtgactg
    25741 ctcgttcctc gacggctttc aaatactttt gttccagata aaacagattg ccgtgaaatc
    25801 tgtctttcat tgacatgtgc tcctggcaaa gcaatgactt catcaaatcg cctagtatgg
    25861 gcaaagtcta gctggaaact agccgctttt tctagtttga ttgtttctcc ggtttcattt
    25921 cttatcttta cagatcgtgt gatcactgct ctatcagtaa atatggtata gaacaaatcg
    25981 aaataaatcg ctgtttctct atccttaagt gaaatcacca acgtttgtgc ctctgacttg
    26041 tccatcacat aagtttgagg caagccttcg atctctggct tcccatctgt aatataatgg
    26101 ctgtcatatc tcagatccag taagtctgat ccattcttgc gctgaatgat cgatgcgggc
    26161 gttcgaaagt ccatgctgcc atgagaagag tattcttgcg gtagtgtatc tttcgacaga
    26221 gaccggtcag gatttaaggg aacatttcct gaaaatccac gatctcttcg gggataattt
    26281 ttattgtctt tgtactgctt gactggtttg ccaaaataaa tatgcgccaa tacattgctt
    26341 tcttctatct ttatcagata agaaagaaat gtatttcgta aatggaagac tcggctcttt
    26401 tcatcaaata agatgaaatt tttatctgtt tttctatgcg taatatccat catttcactc
    26461 ccaacaaaac ggaaaccgtt tacttttttt tataattcct gtatactgtt tactaaacaa
    26521 ttttgtcaat aaaaattttt tatttttttg aggagtgtgt aaaaatggtt ggaattagag
    26581 atgtcgcacg aaaggcaaat gtctctcctg ctacagtttc ccgtgtgttg aataacgatc
    26641 agacgatcca tgtggctcag actactcggc aacggatagt agaggcagct gctgcgttgg
    26701 attatgacat cagtactcgt aaatatatca aaaagagaat gccttcgatt ggtgtgatct
    26761 ctacgatcgg caaagcgagc gaagaacagg atatctatta caaacatttg cgagtaggga
    26821 tggaagaaga agctcgacgt ctgcatttag ggatgaatcg aatctataat ctatctgata
    26881 atcctaaaca atgggaagat ctagatcagt taggtgcggt gatcgttgtt gggactgtta
    26941 cgaaagaatc gattaccgcc ttgttaaaac aaaacatgca aatagtcgtc gtggataacc
    27001 ctgacatcca gcaagaggtc gatatggtgt atgcagatct tgaacggatg actaaaaccg
    27061 tactggaatt gttcttagaa aatggtcata aaagaatagc ctatatcggg ggatataaag
    27121 tcgatatgga tgaattcggt gggaagcaga tttctataaa tgaaaagagg atgcgtgcct
    27181 atcagcattt tatgatcgat catcacttag aaaaatatat gtcttatcat ctaggtggct
    27241 ggacagaaaa acaaggtgag gtacttgcga ctaatctatt agaaaatgaa tcccctcttc
    27301 ctactgccat tcttgtcggg agcgatccaa tggcaattgg tgtctatcgg gcattgaaac
    27361 aaaaaaatat ccaaattgga gaagagattg ttgtagcaag ctttgataat atcgagacaa
    27421 tcgcaaacct ctcccctggc ttgaccagtg ttcaaatcaa tgcgaaatca attgggcaag
    27481 cagctgttcg aatggcattg gagcgaattg acggtatacg ggacgaacca atcattttga
    27541 cttatcctgc acgattgatt gttagggaaa gttttatccc aagaacaaaa aggtattgaa
    27601 agagaggttg tgaaaagccg cttcgtttct tcatactcaa aacgaaacgg ctttttcaca
    27661 acctcttact tatacttatt cacaaaatcc aatgtctgct ggtaacaatt ttgcgattct
    27721 tttagcgtgt aatcaaattg gtattcatgg gtaatctctt tttttgtatc tgtataaaac
    27781 aaggattgca cgggtacctt taattctgtc aaccgatttt ctaatgcttg accttgctcc
    27841 tggaatgagt atgcatttcc gtcagttata tacgttggcg gaaattcctt agaaacatgg
    27901 tcagccacac tagcttcttt caattgctca ctcgttttcc aatcttttgt tccaatcaac
    27961 gaccacgcaa cggttttgac gaaaaacttc ataaatcgat catccgaatg ctgctgtgct
    28021 gttagcttca aatcaacagg tccgcaataa gaaatggttc ctttcagact atcttttgga
    28081 agtagatgtt tgattttcat ttgtttagca tacgcatcat tcgtttgtat agctgcatat
    28141 tgcaatgcga tttgtgaacc tgcactatct ccaccaaaaa ataattgttc catgtctagc
    28201 aatttgtcgt ctttttcttc agctaataaa tctttgatca gttcattcac ttgaagaact
    28261 tgattagggt attctgaatc aggcgctaat tcataattca tcgcaaccac tgcgatgttg
    28321 gcgtctgaaa ctaattttgt cgcaaattct ttgacacccg atttatctcc gccgacgaat
    28381 cctccaccat gtacccaaaa tagaacagga acaggcccct tgctgttttt tggatagtag
    28441 atgtcataag tattctcttt atacttggac tcatacgtca aatctgtttt tttcgccacg
    28501 tttttttctg cttgatcgaa cgttttttta tcagtgattt gaacagtatt tgaaaacata
    28561 tggttgatca agaatgctcc aggtctgggg gaaagctgga aggcaagaaa gattgcaaac
    28621 gcgatcccaa gaatgattcc gaagaaccat ccgataaatt taagaatttt ctttttcata
    28681 aggctccctc agtagttagt attaagcatt ctgattatac catagtgtta tttcctatca
    28741 agctctccta gatgatttaa tgattttccc caaacttctt ctgcaatgct tttgacaaaa
    28801 gaaatttttt ccattgttct tcaaacaaaa cattttcttc ttctgttgaa gcaaatccac
    28861 attgaggact taagcacaaa cgatccaaag gcagatatcg tgtcgcttcg tagatccggt
    28921 caatcacttg ctggtgctct tccatttcgc catttttcgt tacagtcaaa atctaagaca
    28981 actaatttat cttcagagac tttctctaaa agaacaaaac tcccagcacg ttcactatcg
    29041 tattctaaat aataggcacg gacattttct tgagtaaaat aatgggtctg ctacgtcgtc
    29101 atcccccctg atgccgccaa aatcgaataa taatttccgc gtcaaacatg tatatttagt
    29161 gttgaatcat ttgagaatat gaaaataaaa atctttttga tcaatcagtt aatcaaattg
    29221 aaataacgaa aaatctgaga cagaatgacg ttcattctga cccagatttg attttgcaca
    29281 actcaacagt ccttattggc aaatcgccaa aataatgtag actaactaaa aagactaaga
    29341 aacaataaca aaagttaaca cttcttagtc tttctttagc acgtctattt attaaagatt
    29401 ttagggcctt ttctcatgtt tttacgatca ttcttaggtg tttccatttg tttggatcgg
    29461 cctccacctt gtttcttttt gatcaattct ttcatttttt ctttttggtc catttttatt
    29521 ctcctttaac gaatgaagcg actaattcga tatgatgggt ttgcgggaaa agatctacag
    29581 gctgaacttt cttcacggca taacctgctt ctgcaaataa cttagcatct cttgcaaaag
    29641 ttgccggatt acatgagata tagacgattc gttctggtga catttcgatt gctgattcaa
    29701 taaaatgatg atctaatcct tttcgtggag ggtcaactac gatcacagat ggtttgatcc
    29761 ctttttcttg ccattttttc ataacttttt ctgcttttcc gacttcataa tgaacatttt
    29821 cgattttatt atttaaagca ttgaactgtg catcttcaat tgcttctgga ataacttcca
    29881 tcccatacac cttatccact ctgtctgcta aagacaagcc aatcgtacct attcctgaat
    29941 aagcgtcgat cacaatgtct tttttatcta actcggcaaa ctcgatggct gtttggtaca
    30001 gatactctgc ttgttgagta tttacttgat aaaatgactt tgctgagatt cgataggttt
    30061 ttcctaatag ctgatcttcg atatagtctc tgccatataa aactttttct ttgtctccta
    30121 aaatcacatt tgttttttct tcattgatat tttggatgac cgaaaccact tctggcaatt
    30181 cttcatgaat agcttcagca atttccttgg atttaaagaa tttttcttta cgagtaacta
    30241 aaacgaccat catttcatgg gaataatgac cacgacggat aatgatgtga cgaagctgtc
    30301 cttcattttt ttcttcatta tatcctcgta cttggaaccg ttgcaagata tctctgacta
    30361 ctttgattgc ttgatcgatg acaggatctt ggatataaaa atcttcgata gggactagat
    30421 catggctatt ctttttgtaa aaaccggtgg ctaactgtcc atcgatacgg cgaacaggga
    30481 tctgtgcttt attacggtag ccgaatggtt ctgccattcc gaccgttgga agtacaggaa
    30541 cttctggcat cttagcaatc ttcgccatta catgctctac ttgtttttgt ttgaagacaa
    30601 gctgttgttc atacttcaaa tgggctaaag gtgcgatacc tatgcgaagt aatgcattgt
    30661 tttctacagg ctgacgatca ggtgattgtg taataaatgt ttcgactttt ccatatccaa
    30721 acttattgcc tgcttttact actagtattt ctacttcttc tccagggagt gcattttcga
    30781 taaataatgg ataaccatcg atttttgcta cccccattcc ttcataactc aagtcgacga
    30841 tttcgactgt atatcgttcg tttttcttta ctatttgctg ttctgtcata ttttgctcct
    30901 ttaagcctat ttttcaatcc tactgaactc tactataaat cagctaatta cacaactaaa
    30961 accggcactg accttactac gttcagcacc ggcttttgat taattctttt gattaggtgt
    31021 ttcgtctgct atttttccgt caccgtcgat atcttcatcc gttaaacgtt cgacttcttt
    31081 tacgaattct ttgctgacaa cctctaattc ttcttcatct gtacctgtga ttgcttcatc
    31141 aggaatctga tcggtattgg caaagaactc aatgtgttga tgaagatttt ggaaatgcat
    31201 cggtgcttcc ccgccgtatt caccatctaa gttgatcatt actggtcgat tgatatcatc
    31261 ttctgtttca acgaacaaac tgcttgtttt ggtatagatt attcttggat catcgacatg
    31321 tcgaccgcca ttcagcatga gtgctgcaat atgcaagatc tcaaagatat ttgctgtctt
    31381 cacaatgatt aaagaaaact tcccatcatc caatttggca tctggcgcga tctgttcgaa
    31441 tcctccaaca gagttcgtca gccctaggaa aaacatcgaa gcatttccga tatattcccc
    31501 ttcatcatac ttcaagcgca ttttgattgg ctttactcga gggagcatct ctgctccttt
    31561 cgcaagataa gcaagataac caaaaatact ctttagttct gatggaactt cataagtcaa
    31621 ttctgtcaaa tagccacctg cagcgatatt gatgaagtaa ctgttatcag aaaggccgat
    31681 gtccattttt acggtttggt gtttttttat cacttctgct gctgctttaa tattatctct
    31741 aggaattttt aatgctcgag catagtcgtt cgttgtacca gctgggatga tggccatttt
    31801 aggacggtgt tcaagcggag cgattccatt aaccacttcg ttgattgtgc catcgccacc
    31861 agctgccact actagatcaa aacctagttc tgcgactcta catgcttcat tcttggctga
    31921 atctggttct ggtgtagttg cataggcgct ggcttcaaat cctgcttctt ctaatgcctg
    31981 taggatatcc gccaaatttt ttttcaataa ttcttttccc gaggtggggt tataaattac
    32041 gcgtgctttt ttcattttca gcctctatcg tttcgctaat tcatctttaa gtagcttatt
    32101 cacgacacca ggatttgctt gtcctttagt tgctttcatg atttgaccga ctaggaagcc
    32161 aaccgcacga tcttttccat ttttgaaatc atcgacagat tgttggttgt tatccaaaat
    32221 ttcattaatg attggcagta gtttgttcgg atcagataat tgcacccaac catttttttc
    32281 aactacttct tgggcttctc cgccatttgt gatcaattca cggaagactt ttttggcgat
    32341 ttttgagctg atcgttccat cggcgatcaa acgaatcatt cctgctaagt tctcaggtgt
    32401 caattttgtc tcagctaatt ccagtttctc actgttcaag taagcagaaa cttcacccat
    32461 caaccagtta gatgcttgct tggcatctgc accattttcc aaaacagctt caaaaaaatc
    32521 agacatttct tttgtaagtg tcaataccat tgcgtcatac tctggaagtc caaactcacg
    32581 gatatagcgt tcgcgacgag aagctggcat ctcaggcaaa ctagctttta ctttttgaat
    32641 ccattcgtca tcgatcacga atttcgggat atcaggctct gggaagtaac gataatcact
    32701 agacccttct ttgacacgca tcaaaactgt tttattcgtt gtttcgtcaa aacggcgtgt
    32761 ttcttgttgg atttctcctc ctgaaagaag aacttttgct tgacgttttt cttcaaaagc
    32821 taaacctttt ttcacgaaag ataaagagtt caagtttttc aattctgctt tcgtaccgaa
    32881 ctcttcttgt ccataagggc gaagagaaat attggcatca cagcgcatag agccttcttc
    32941 cattttcaca tcgctgactt ctgtaaattg gataattgat cgcaatgctt ctagataagc
    33001 gtatgcttct tctggtgaac gcatatcagc ttcagaaacg atttcgatca aaggcgtgcc
    33061 ttgtcggttc aaatcaacgt aagaataacc gccaatacca tgcatgtttt ttccagcatc
    33121 ttcttctaaa tggacacgtt cgatccggat ttttttcttt ttaccttcta cttcgatttc
    33181 gatccatccg tcatgaccga ttggttgatc aaattgggaa atttgatatg cttttggatt
    33241 atctgggtaa aagtaatttt tccggtcaaa atgagtatct ttagagattt cacagtttaa
    33301 tgccaaagct gctttcatcc cgaattccag cgcagcttta ttcattactg gcagcacgcc
    33361 tggataaccc cagtcgatca cgtttgtatt gctgtttggt tctgcaccaa aatgagctgg
    33421 cgctggagag aaaatttttg agttggtttt taactcgacg tggacttcta gtccaatgac
    33481 tgtttcaaaa ttcattcgtc tttccctcct aaaatagctg gttgttgttt atggaattct
    33541 gttgcttgtt caaacgcata tgccgcttga tacatcgttt tttcatcaaa ataattaccg
    33601 atgatctgca atccaactgg taatccttct gataaacctg ccggtacaga catacctgga
    33661 agtccagcca agttgacagg gatcgttaag atgtcactca tgtacattgt gattggatcg
    33721 ttaatgttct caccgattcc gaatgcaact gttggtgaag atggcccgat gatcaagtca
    33781 taatctgcaa agactttttc aaaatctcgt ttgatcaatg tacggacttg tcctgctttt
    33841 ttgaagtaag cgtcataata acccgcgctt aaggagaatg tacctaacat gatacggcgt
    33901 tttacttctt caccgaatcc ttctgaacgt gagttgacat atacatcatc taatgatttc
    33961 acattttcag agcgataacc gtagcggata ccatcgaatc gttgcaaatt ggaacttgct
    34021 tctgatgagg caatgatata ataaacagcc acaccgtatt ttgaatgagg caagctgact
    34081 tcttccacaa cagcacctaa tgaacggaat gtttctgctg ctttcaatac agcttctttt
    34141 acgccagggg ccacaccttc gcctaagtat tctttaggta aagcaatctt cattcctttg
    34201 atgtcgcctg tcagtccctc tgcaaaatca ggaactgaaa cgcctgaaga agtaccgtct
    34261 ttttcgtcat aaccgctgat agctgtcaat gctaatgcat tatctttcac cgtacgggtc
    34321 attggtccga tttggtcaag agatgaagca aaggcaatca aaccaaaacg tgagacacgg
    34381 ccataagtag gtttcatccc tacgatgccg ttaaaagctg ctggttggcg gatacttccc
    34441 ccagtatcac tacctaaaga aactggaatt tgtccagctg caactgctgc tgcagaacca
    34501 ccagaagagc caccaggtac ttttgttggg tcccaagcat tttttgtttt cttgaaataa
    34561 gaagtttctg tacttccacc catagcaaat tcatccatgt ttagcttacc aacagggatc
    34621 atatctgctt gatagatttt atccatgacc gttgcatcat aaattggttc aaaattgtaa
    34681 aggatcttag atgcagcggt tgtcaagata tcttttgtta cgatattatc cttgatacca
    34741 attggaatac cagcaaggac gttgctttca tcaatccctt tttcatctaa agcttttgcc
    34801 tgttccaatg ctttttcttc gttcaatgta atgaatgcct cgacctcagg ctctgtttct
    34861 ttgattcgtt caaatgttgt ttttgtcaaa tcttgtacag tgatttcttt cgatacaagc
    34921 atatgatgca gttcttctag agaacgattg tgtaattctg tcattatgcg ccagcctccc
    34981 cattatcgat gattgcgggt actttgataa agccgtcttc tgtttccggc acatttttca
    35041 ataattcatc gcggttccat ccagcttgcg cccgatcttg tcgcatcacg tttactgttt
    35101 caaccacatt cgacgtaaat ggaacacctt ccgtatctac ttcttccagc atttcaacca
    35161 tatcgatgat tttacctaat tggtcggtaa aacttgcaag ttcttcatct gcgaacgcaa
    35221 gttttgctaa tttggcaacg tgtttcactt gttcttcact aatcgccata ctaccccttc
    35281 tttctaaacg agttaaaaac tttgctctct attttaacac aagagtgccg tgtctgaaat
    35341 tgtttattga aatttgtctg aaaaaactca tttttcattc ttctactgag cgacttttag
    35401 ccaatgcatc tgccatttga tcttctgtcc aaacttcgat tcctaattct tgtgctttgg
    35461 tcagcttgct gccagcatct tctcctgcta ccacgatatc cgtttttttc gatacgctac
    35521 cagtcacttt cccgccaaga ttttcgatcg tctcttttgc ttcttcacga gtaaatcgtg
    35581 ttagtttacc agtaaggact actgtttttt ctttaaaagg agactcgact tcttgcaatt
    35641 gggcatttcg aagccctttg tattcgaaat tgacgcctgc ttgttttaat tcattcatca
    35701 attcatggac ttcttcgtta ctgaaataag tgactacact gtcggcaatg atatcaccga
    35761 tcgtatcaag cgcaataatc gactcatagt cacttttact caatgtttct agatcaccga
    35821 aatgttctgc taaaattttt gctgctttcg ctccgacatg tcggattcct aacccgaaaa
    35881 tcaaccgctc gaccgaatta tctttgctgt tgtcgattgc tgtcaaaata ttatttgctg
    35941 atttttcttt tattttgtcc aacgtcaata actcttcttc tgtcaatttg taaagatcag
    36001 caacatctgc tactagtttt ttatcataca tctgttctag tacgcgcggc cccaacccat
    36061 cgatattcat cgcatttctt gaaacaaagt ggttcaaacc ttctttcatt tgagcagggc
    36121 atttaggatt gatgcaccgt aaggctactt cttcatccaa atggacaagt tcacttccac
    36181 aaactggaca atgtgtaggc aattggtatt cttcgctgtc tttaggtcgt ttatctaaca
    36241 cgacttgcga aacttctggg atgatgtctc ccgctttata aatcagaact gtatctttca
    36301 agcggatatc cttcaactta atataatcac cgttatgcag gctggcacgg cttactgtcg
    36361 ttcctgctac tcggactggt gtcatgatag ctgttggcgt tactacccct gttcgaccga
    36421 ctgtccattc gatgttttcg attagcgttt caacttcttc tggcggaaac ttgtaagctg
    36481 ctgcccatct cggtgctttg atagtaaaac caagttgatc ttgtagcgag aattcattca
    36541 ctttgatgac gattccatcg atttcatagg gcaaatccac acgtttgtca tgatattctt
    36601 caatgtatga ccacacttca tcaatcgaat gacatagacg tttttcccga ttcgtgtgga
    36661 atccaatttt ttctagctct tctagcgcat catactgggt ttttgcttgc ataggaccga
    36721 aatcagctac tgtgtataaa aaagtgtcca gatttctttt cgctgtgatt ttagaatcta
    36781 actgacgcaa agaaccagct gcggcgttcc gaggattcgc aaagatatct tttccctctg
    36841 cttctctgtc ttggttcaat tggacaaatg accgttttgg catgaaacat tcgcctctta
    36901 cttcgatgtt cattggttct tttaatttga ttgggaccga ccggacagtt tttaggtttt
    36961 cagtgatgtt ttctcctaca gttccatccc cgcgagtcgc acctctcacg aaatttccat
    37021 cttcataacg caaagaaacg gaaagtccat ctatttttag ttcacagcaa tagtcaacta
    37081 cacgtcccac tgctttttgt acacgttgat caaaagctat cagctcttct ttgctgaaaa
    37141 cgtcgttcaa actgtaaagc ggaatatcat gagtgacttt ttcaaaacct tctaatactt
    37201 ttccgcctac tcgttgagtg ggagaatcag acgtgatcaa atcaggatat tgttcctcga
    37261 ttgctactaa ttctgcgtat tctttatcat aaacatagtc ttccactgtt ggtttatctt
    37321 ctacgtagta ttcgcgagac cattggttca gtcttgtacg aagttcttct gctcgtttag
    37381 cagcttcgtc aatcgtcatt tcatgttcca tcatttaccc tccactacta agctttttca
    37441 attggcgcaa aggcagccaa aagacgtttt actccttgac ttgggaaagc cacatctagt
    37501 tctagatcct tcgacgtccc gcttactcgg acgactgttc ctagacccca ttttttgtgt
    37561 ttgactttat ctccaggttt ccagctgtta ttttcccctc cagtagcact tttagaggga
    37621 acaaccgctg tctctggctg tttatagcta tatgctgttt ttttagcgaa tacaggcgtt
    37681 tttttaggcg taggacgcat gccttcgatt tccagtaact cctgatcgat ttcttctaca
    37741 aaacgagaag gtcggttata ttgcgtccgt ccatataatg ttctggagaa agcatttgtt
    37801 aaatacaacg cttcttctgc tcgcgtgatc cccacatatg cgagacggcg ttcttcctcc
    37861 agttcgcttt cttccatcaa tgctcgagat aaaggaaaaa ccccctcttc tagaccaatc
    37921 aggaatacaa ctggaaattc taatcctttg gctgcatgca aagtcatcaa tgtcacttgg
    37981 gaagcatctt cttctaaatt gtcaatgtcg gaaacaagtg ccagatcatt taagaagacc
    38041 gttagttttt cttctggtgc atcagcgtct tcttcatttt gctgttcgaa ttgtttgtcg
    38101 aattcttgtg tgaccgttaa aaattcctcc aagttttcga tgcgtgcttg tgcttctaat
    38161 gtattttgga tttttaagtc ttctagatat ccgctacgat ccaaaacctc tttcgtcaat
    38221 tctgtcactg tcaagtatgg gatcatctga gtgacttctt ggatcatctc tccaaatgca
    38281 cctaattgct gtcctgcttt tccaccgata tttgccagat ccacattttg cgctgcttca
    38341 agcagtggcc attcatgaag agaagcaaaa ctgcgtagtt tttcgataga tcctggtccg
    38401 atgcctcgtt tgggcgaatt aacgatacgc tcaaaactga ttgaatcttg aggatttacc
    38461 agcacattca aatacgctaa aatatctttg atttctttac gatcgtagaa cttgtgccct
    38521 ccaaccattt tataaggaat atttgctttc aacagagttt cttccattac acgagattgt
    38581 gcgttcgtcc ggtacaagat ggcaaagtcg ccgtaattcc gatgattgct tcgaatctct
    38641 tcttgcatcc ggtctacgat aaaacgggtt tcgtctcgtt cattatctgc acggtagtaa
    38701 gtgatcttgt ttccttcttt gttttctgtc cacaagtttt ttggttttcg attgctattg
    38761 ttttcaatga cttggtttgc tgcgctaagg atgtttttcg tggagcgata gttttgttct
    38821 agcaaaatta ctgcagcatc tggataatct ttttcaaaat ccaagatgtt ctgcatatct
    38881 gcaccgcgcc agccgtaaat actttgatct gcatctccca cgacgcacag gttcctaaat
    38941 ctacctgcca ataaattgac tagcgtatac tgtgcgtgat ttgtatcttg gtactcatct
    39001 acatgaatat aatggaattt gttttgataa tacgtgagtg aatcaggatg ttcttcaaat
    39061 aatcgaatcg tattcatgat caggtcgtca aagtccatac attgattata ttgaagctct
    39121 ttttggtaag cagcataaca ttttgccgca atctcttcgt aataactgcc ttgcattttt
    39181 gcaaaatcct gaggggtctg caggctgttt tttgcttgag agattgttcc taatatactt
    39241 cgaggatcat attttttcgg atcaatgttc agttctttta gtatccgttt catcaaggtc
    39301 agctgttcag aagaatcgat gattgtaaag ttccggttat acccgataaa atcaacatct
    39361 cttcttaaaa tacgcacaca catcgagtga aaagtcgata cccagacatc ttcgcctcca
    39421 gatgcgagaa tcgcattgac acgttctttc atttcttttg cagccttatt ggtaaaagta
    39481 atcgctagga tgttccaagg attgacttct ttttcctcaa tcaaataggc aatgcggtgt
    39541 gtcagcactc tcgtctttcc acttcctgca cctgccatta gcagtaatgg tccgtcagtg
    39601 tgaagaacag cttctttctg acgggggttc atcccgttta ataattcagc tttggatgac
    39661 attaacaaca tcctttcttt tatcaatctt gttaattata gcattttctt ggctttcatt
    39721 aaactagcta gttcaaaatc atttgtttct ttttatggat ttttccttgt caattcgttg
    39781 atttcgcgct aaattaaaag aaagcgaggc gtttgaatgg aaaaaaaagg aaaaatcaag
    39841 aaattagata agactcagat atattttatt ctaaaaggaa tatttgttgg actgctgaca
    39901 ggaatcgtcg ttagcctgtt tcgattatct atcgaaaagc taagcgattt agttcgaagt
    39961 atctatgaaa tgagtcggga acagccgatt tacttaattg gcctagctat ttgctgcatc
    40021 cttgcagcat ttttcgtggg ttatctggtc aaaaatgaac cggatatcaa gggaagcggg
    40081 attccacaag tagtgggaca gcttcgcggg gaattatcaa tgaactggtt ctctgtttta
    40141 tggaaaaagt tcattggcgg tgtactttca gttggtgcgg gcctttttct aggtagagaa
    40201 ggaccttcta tacagcttgg cgcatccatt ggacaaggag ctggacaatt gtttcggagt
    40261 ccatcttcag aagaaaagat tttaatttct agcggtgcaa gtagcggact tgcagcagca
    40321 ttcaatgctc ctatcgcagg attactcttt gttttagaag aaatccatca ctccttttct
    40381 ccacttgttt ggctgacatc atttgcttca gcaatcactg ctaacttcgt ctctctttac
    40441 tttttcggct tgagacctgt tttatatcta ggtgaccttc cttctttgcc gttgaaatat
    40501 tatggttcct tagtgatctt aggagtactt ctaggcatct taggttttat ttatcagaat
    40561 gtcttgctgg ctttgccaaa attttacaac cgattgcctt taccagctta ttttcacggg
    40621 ttcgttcctt tcttgctgat tttgccaatt ggttatttat ggcctagtac tctaggcgga
    40681 ggcaatcaaa tcgttttagc ttttggacat gcttccttgc caatcattat gattatcggt
    40741 ttgtttgttc ttcgattcgt tttttccatg atttcttacg gtgggggtct tccagggggg
    40801 attttccttc cgatattgac attgggggct ttaatcggta gcttttatgg aaacattatg
    40861 gttgatttcg ggatggatcc aatctacatc aaggattttg tcgttattgc catggctggt
    40921 tatttcacag cgattggaaa agcaccgctg acagcaatta ttttagtgac agaaatggtt
    40981 ggctctctta atcatctgat gcctttagga ttggctgctc ttgttgctta tatcgtcaat
    41041 gaccttctcg gcggtaatcc aatttacgaa tcattattgg aacgtctact tggaaatact
    41101 cagaccgcct cactaaaagg tgcaaggaca acttttgatt ttccagtaac ggcagagagt
    41161 tctttagacg gattaatggt acgagacttc aattggccta aaaatatgct gctaatctct
    41221 atacgcagag gaaatcagga aatcttgact cacggtgata cgatcatgaa tgtaggagat
    41281 attttggtta tcttgactga tgaagcaaac ttgccaagaa tcaagcagga aatcgaggct
    41341 aaatcttcta tcacaaattt gaagcaatca ttttaacgaa catggaaaaa ctattttaca
    41401 acaactattc atctacacta cttgattcta tatgattcta taaaaaaaga agaggctggg
    41461 aaaacccagc ctcttctttt ttatttctat gcttctgcta ctgcaggagc ttttctcaat
    41521 aaaccataaa tcacaccaga tacgactgct ccgatagcaa taaacaccaa atacatgacc
    41581 gggtgactca gtaagaatac aacgaaaatc ccgccgtgag gagccagtaa tttgattccg
    41641 aaagctccga ctaatccgcc agttaaagca gaaccaataa taaaacttgg aatggcacga
    41701 atcgggtcag ctgctgcaaa tggaatcgat ccttccgtga caaacgatag tcccatcacg
    41761 atgttcgtca aaccagcatc ttgatctgtt ttgctgaatt tattcttaaa caaacgtgta
    41821 gcaacgaaaa ttgccaatgg aggtaccatt cctccagcca taacagaagc catcacgaca
    41881 cttccgccag ttgcaacagt cgtagctaaa gttgctgtac caaacacata agcggctttg
    41941 ttgattggac cgcctaaatc tgccgccatc attccagcta ataatgcacc aagtaaagca
    42001 gcattcgaac cactcatact tgtcaagaaa ccgttcaatg catcattgat tgctttcata
    42061 gggacattga ccagcaacat caagaaacca gtaatcagta agccaaacac tggataaaag
    42121 aggatcgttt taattccatc caatgatttt ggtaagttct tgaataattt acgtaaaaat
    42181 acgatgacat aaccagctaa gaatccacca atcagtgcac ctaagaatcc tgcaccgcct
    42241 gtattagcta atgcaccagc agcaaatcca acgatcaaac cagggcggtc accgatactt
    42301 gaagcaataa atcctgctaa taccggcagc atgaaaccaa atgcagcttg tccgatttgg
    42361 ttgaaccagc tagcagcttg attgtaattc cctagatttg ataattgatc atgtggcacg
    42421 ccaataaatt ggtcgatcat gaaagaaagc gcaatagcga ttcctccacc aataacgaaa
    42481 ggtaacatgt gtgatacgcc gttcatcaaa tctttgtaaa ttctttgtcc gatagtacca
    42541 tccgcatttc cttcttctga actgctttct tttccatcac catggaatac tggtgcagta
    42601 ccactaaggg ctagattgat caactcttct ggtttacgga taccatcact gacaggacga
    42661 ttgatcagtt cttttccgtc aaagcgattc atctctactt ttttgtctgc tgcaacgatt
    42721 acaccttctg cacgggcgat gtcttcagca gtcaatcggt ttttgattcc ttctgaaccg
    42781 ttcgtttcga ctttgatctc cacacccatt tctttcgctt ttttcttcaa agcatcttct
    42841 gccatgtaag tgtgagcgat ccctgttgga caagctgtaa cggcaacaac gaattttctt
    42901 tctggatgag cagaagcttc tgcagcttgt gtttcttgtt ctgctttttc ttctgcttct
    42961 ttttgctctt cagctgtttg gaacaagtct tggacttctt ctggtgtatt tgcttctttt
    43021 aattttccta cgaaatctgc atcaatcagc aatcgagata gtgctgctaa tgcttgcaaa
    43081 tgtgtgtcgt ttgcgccttc tggagctgcg atcatgaaaa acagataagt tggctgtccg
    43141 tctaaggctt cataatctac tcctttttgg ctttttgcga ataaaactgt tgcttcttta
    43201 actgcactgt ttttcgcgtg aggcatggcg attccatcac caagacctgt agaagtttgt
    43261 gcttcgcggg ctagaattcc ttctttatat gtttcgatat cggaaatccg gccggcatca
    43321 aacatttttt ggaccatttc atcgattgcg ccttttttat cagtcgcttg caaatccatg
    43381 atcatcgctt ctttgaccat taaatccttg atattcatga ttatcctcct tgaaactaat
    43441 caatttttgt gatttcaact tctggcagca actcatcaat caattcgcga gctgccaagt
    43501 catcagaaaa agctgttgcg cttccgcaag caactcccca tttaaaggct tcgattggat
    43561 ctttcgtacg gctgtaagcc ccaataaagc cagcaatcat tgaatcccct gcacctactg
    43621 aatttttcaa tgggcgaact aatacattcg ttttataagt tccttgttcc gtgaagagca
    43681 aagcaccgtc tccagccatt gagatcagca cattctgtgc cccttcagct agtaattttt
    43741 tcccatacgg aatgatttct tctacagatt ggaaggttgt gttgtaaagc tcagctaatt
    43801 cgtgattgtt tggtttgatc aacaacggtt tcttaacaag tgcatttttt aggtcatcac
    43861 ctgttgtatc aatcacgaaa tctgcacctt tttctcgaat gatatcaatc agttcttgat
    43921 aaaatccttt tcgcaggcta gctggcgcgc ttcctgacaa tacgacgata tctccttgtc
    43981 ctactgttcc taatgcttgc ttcaactcat cgatttcttc atctgaaata gatgggccta
    44041 agccattgat ctcagtttca gtgtctgatt ttagtttgat attgattctt gtatcttctt
    44101 ttacggttgt gaacaaagac tggatttgtt ctttttcaag ccacttcgaa ataaattcgc
    44161 cagtgaaccc tcctaaaaac cctagtgctg tagattgaga atcgatccgt ttcaaaatac
    44221 gggaaacatt gatccctttt cctccaggta acttgaagtc ttccttcatt cggttcaaat
    44281 cgcctagttt taacccatct acgcgtacga tataatcaat tgatggatta agtgttactg
    44341 tgtaaatcat tgtattgcct ccttaattgt tgtcttttca cggaattcat taacgaattc
    44401 taatggacaa cgctcggtta tgatcgtggc tgcttccaaa ggtgcaacag aggtgaagga
    44461 caactggttg aatttggtat gatcaatcaa tacaaatgtc tcttcggaat ttttcatggc
    44521 taaacgttta acagctgcct cttctggatc cggagtcgtg aatccttgtt caagatgggc
    44581 accgttcatt cccataaatg ctttattaaa atgaaaattt tttagttgtt ctatcgcatt
    44641 agatcctaaa acagcattag ttgccagctt tatcgttcca cctaaaataa tggtagcaag
    44701 ctccaaatca actaaatttg ccgcatgttg aacggaattt gtcactactg tgatctgttt
    44761 tccttgcaga aaaggaatca tttctaacgt agtagagcca gcgtctaaat aaatgacatc
    44821 ttcgtcatta atggtttcag ctgccgattt ggcgatttgc tgtttttcgt gaacgttttt
    44881 gagtgatttt tcactcatat tctgctcaaa attcaataat tgctctttct tagctccgcc
    44941 atgaactctt tctagaaacc cactattctc cagctcttgt aaatcacgtc ttatcgttga
    45001 ttcagaagca ttcaaaaggt ttacaagatc ttgtgatttc acgattttct gctgttcaag
    45061 caagtctagg attttttgtc tgcgttcctc tgtaagcatt ttcacgatca cctcaataca
    45121 aataatagca tatttttttt ctactagcaa tataaatcag tcaaaaacgt tcataaaaat
    45181 tataaatcag tcaaaaaaaa ataaaaaacc agtcagaaag actggttttt tatttttttg
    45241 tctatcatca agccgtttta tcgtttttta ttttcgcttc ttgttttttt catataaaca
    45301 gaagcaggtt ttctttcctt atgaagaaga taataataaa gtcccatccc gccaaacatg
    45361 accaaaaaac cgatgactga tcgaatagga aaattaggaa caaaaaagag acagacggat
    45421 aataatgatg ccggaaccat tattcgataa tagagttgtt tatccatttt cttcacctca
    45481 tattttttgt aaagtaatca attgttctaa ttctcggtaa tctaaaaatt gcacacgcag
    45541 atgctgctca cctttcttga catcgaattg gatacttcgc cgctttttct ttattcgttt
    45601 gaaggtaaca tcagagagtc tatgtttgat ttcctttaac ggtaattctg acagggattc
    45661 tgaaggaaca tagattctta aggataaaag atactcttga tatttttcat ctgttacctc
    45721 tacttctaga tattccctaa tataataaca ataagcttcg attccattat ccaaagcaac
    45781 agtcggatcg ataactttat agcacatcat gcgtgatcga ttctcgtcaa cccaaggaaa
    45841 atacgcctga gcatttagtg ttccgcgaac acgcctttct ttataatctc cttgttggat
    45901 atcataactt tttgttacaa aaaatgaagc attcttttta ggaacaggtt gcatttgtgc
    45961 attctgatac tggacagatt ggatcgtatg gtcagtgtaa atgtattcaa cgaatggcgg
    46021 tttcaataaa ccgacaccat tgtcccaagc aagatgatca aacccaagct ggtccctaaa
    46081 ataggattga aacatttgat tgtttggctt ttgccagtta aaggggtctc gataaagctg
    46141 gatacatatg acttcgtttt ctaattcctt cactttttct acttgttgga tgtcgagaaa
    46201 aatcggttta gggatgattt gatgataata gcgactataa tacatttccc aacacgaagt
    46261 gaaacataaa ccttggtaaa aaagatcgta tcctggaaat tggttgcaat cgactactac
    46321 ttccccgttt tgttgtttca ttttaggtaa atgttcgatt tcatcctttt tctcaaacaa
    46381 tagccgttga gagatctgtt ttgtattgtg atagataaac tcgctataag ctcgcatata
    46441 ggcgaacacc ccatgagcaa tcattttttt ctgaatgaag tctcgaatca aaaattgctt
    46501 ttctttgaaa acagagatag acaatacatt ttttatcaat aattgattat cttccaagcg
    46561 aaaactacat tcgttctcgt catttcctac aactaaccaa ctttcgtctc ttaccgatgc
    46621 tagcattatt ttttcttgaa gctttcgaag atcatacgga tccgcttgtt gtgtgaaaga
    46681 gcggataaat gttggctgga tcaacagacg tgtcaaaata gcccaatcgc ttatttcctg
    46741 ttttttttct tcttcattta agtctgtgag ctggatagtc atcaataatt tatcttcgcc
    46801 aatcattttc tcacctcttt caaatctttt tataatttaa ttataaccaa tgataaagaa
    46861 aaactaaaac tttatgtctt agaaaccaat ttggacgatt acatgataga ctaagtaaaa
    46921 agaaacaaag aggtgtttac aatggggttg aaatttgaac gtacagataa tttagataaa
    46981 cttttcgaat catttgctgt cgatccggaa aaaacagttg caccaaaaga agagatcgat
    47041 cgatttctaa aaccacagcc acaaaatgaa aagaaaaaag acaagaaaca aaagtaagtt
    47101 aagctcaaaa aaagacttac cagccaaact tttatgaaag agtttgactg gtaagtcttt
    47161 tttattcttt ttatgattca attgcttgtt cgattgctgc ccacgcttct tcttttccct
    47221 gttttgtaac ggaagaaaag agaataaacg tgtcattgcg atcaaaatcc aacgcttttt
    47281 tgatttcgct ctcatgcttg ttccatttac cacgagggat cttatctgcc ttagtggcaa
    47341 caacaattac agggatatca taatacttca aaaattgata catctggata tcttcttgag
    47401 aaggcttatg acgcaaatct actaaagaaa cgaccgcacg tagctggtct ctagtcgtta
    47461 aatatgtctc gatcatcttt ccccattttg ctcgctcagt tttggatact tttgcatacc
    47521 catatcccgg aacatcgaca aaatgtaatg cgttctcgat caaataaaaa ttcaatgtct
    47581 gggtctttcc tggcttaccg gaagtacggg ctaaattctt tcgatcaatc aatgtgttga
    47641 taaaagaaga tttccctaca tttgatcgtc ctgccagagc tatttctggc aattgtgtat
    47701 ctggatattg ttttggtgcc actgcactta tgataatttc agcttgatgg actttcatgg
    47761 tctctctcct taacaaggtg atgaataaag caagcggctg tcgcaaaagc caaaatgact
    47821 tttacggcag cctcttttca tcaacccgct tttttctctg aatctttata gattactgtt
    47881 ggctcaccag acaattcagc tgcttctttt gtgataatca ctttttcgat gttttcattt
    47941 gaagggatat caaacatcac atccatcatg atttcttcaa tgatggaacg aagtcctcga
    48001 gcaccggtat tgcgttcgat tgcttttttc gcaatggctc tcaacgcttc aggttcgaat
    48061 tcaagttcgg tattatctag agaaagaagt ttttgatatt gtttgactag cgcatttttc
    48121 ggctcagtca agatccgtac cagatcatct gttgtcaatt tctctaaagc tgccataaca
    48181 ggtaatcgtc cgataaactc agggatcaat ccgaatttta gtaaatcttc aggaatgata
    48241 tgctgcatga cactttcgtc ttcagaaagt ttttggtttt gtgtaccaaa tccaatcgtc
    48301 ttttcgccca tacgattctt cacaatcgtt tcgatgccat caaatgcacc acctacgatg
    48361 aacaatatgt tcgtcgtatc aatctgaata aattcttgat gtggatgttt acgtccgccc
    48421 tgaggtggca cacttgcaac agttccttct agaattttca gcaaggcttg ttggacacct
    48481 tcgccggaaa cgtcacgagt aatagataca ttttcgcttt tacgcgcaat tttatcgatc
    48541 tcatcaatat agatgatccc tttttctgca agttccacat tgtaatcagc agattgaagc
    48601 aatttaagca aaatgttttc tacatcttcc cccacataac cagcttctgt caaacttgtc
    48661 gcatctgcga ttgcaaaagg tacattcaat gtttttgcta atgtttgtgc taaaaatgtt
    48721 ttaccagacc ctgtaggtcc gatcaagcag atattgcttt tttgcagttc aacatcatct
    48781 gtgttttctt gtgcttgctg attgacacgt ttatagtgat tgtatacggc aacagctaac
    48841 gagcgttttg cgcgatcttg tccaatcacg tagttattca atacttcaag aatttctagt
    48901 ggtttaggga cttcagtgaa ttcgcgtaca gcttcttcat aaaattcttc atcaatgatt
    48961 tctttacata ggtcgataca ttcattacag atgtagaccc ctggaccagc tacgatcttt
    49021 ttcacttctt cttgggtttt cccgcaaaaa gaacagcgta cggtctcatt gctacttgga
    49081 ttgtcataca tggtcttcac cccttaactt gcaaaaagta tgcaagaaac tatcaaagac
    49141 tttacagtct tttactatca taacatagtt tagtaaaaag aaaaagtgtt taacaaagga
    49201 tcaactgacc tatacctttg cattttttac tcttaacaaa aaataaggaa ctgtgacaaa
    49261 atcgtgtcac agtcccttat ttcgaataaa caattttcta gaagtagctt cttcagaaat
    49321 gatttattac ttgatttcta ttctttcgct tctgtcgctg tacctgtgat caattcgatt
    49381 gcttttttca ttgtcacatc atgttccagc atatcttctg tcaacacgcg tttgatttgt
    49441 tcgattggca tgttgtatgt ttctgataat tcttggattt ctgcattgat ttcttcttct
    49501 gttgcttgaa gattttcagc agctgcaatt gcttcgatca caagatttgt tcttgtgcgc
    49561 atttctgctt caccttcgaa ttgtttatgc aagtcttctt ctgtagaacc agttaattga
    49621 tagtacattt caggagagat accttgacgt tgcatgttgt tcaagaattc gtccattgaa
    49681 cgatgtactt catcatgaac catcacgtgt ggcaattcaa cgatttctgc gttttcaaca
    49741 gccatacgga tcgctgcttc atcttttgca tcgtccgctg ctttttcttt tgtttctact
    49801 aattctttgc gatatttttc tttcaattcg tctaatgttg atacttcgtc atcaacatct
    49861 tttgcgaatt cgtcatctaa ttcaggcact tcttttgctt ttacttcatg gattgtcact
    49921 ttgaagactg cttcttttcc agcaagatct tctgcttgat aatcttctgg gaaagttaca
    49981 tttacatcta gactttcacc tgatttatga ccaactaatt gatcttcaaa acctggaatg
    50041 aatgagcctg aacctaattc aagtgagtaa ttttcgcctt ttccgccatc aaacgcttcg
    50101 ccatctacaa agccttcgaa gtcgattact actgtatcgc cattttcagc tgcagcatct
    50161 tctttgataa ctaattcagc ttgtgcttct tgttcacgtt tgatacgtgc atccacgtca
    50221 gcatctgtta cttcacggtc ttgtttttca acaactagat ctttatattg tcctaatttt
    50281 acttctggtt tcactgttac ttcagcagtg attacccagt cttgaccttt ttccatgctt
    50341 tcaacatcga tttttggttg agaaacagga tcgatgccag cttctttgac agcagcttca
    50401 taagcttctg gtaatacagc attcaatgca tcttcatata aagcttcttc accgtacata
    50461 cggttgaata cttgacgaga aactttccct ttacggaatc caggtacgtt caagcttttt
    50521 ttcactttat taaacgcagt tgttaatcct ttttggattt cgtcttgcgc aatcgtaaat
    50581 tttaaaacac catcattcgt gccttttttt tcaaatgttg cagtcattta attccctccg
    50641 agtttagaaa ttcattttat acatcaaaaa cgtttcatgc atacctttaa atagtacact
    50701 aaccctttca aattgtaaac atattgaatg gatagaatcc ttgtttttca taacattttt
    50761 atctttttca ctgataaaat ctaatggaag aagatcattt gctgcaaaat tttttctctt
    50821 ttttttaaaa aatcttgttt ttcatcttct gtaccttcat acatcgtttt tcctgtacac
    50881 gcctctacag tcagttgaaa ccagcgcttc gggtcttcga tgtatgtatc atagacagga
    50941 taaacaattg cagattgcaa cgtgaattct tcttttaaca cggaactaag cagcgcatct
    51001 tcattcccgt aacgatctga aaatacacat aattgctggt aacttgcctg ctgctgcggc
    51061 tttggcagat cttttggtat gactttgact aacttttctt caatcgtcaa atagaaaaca
    51121 ggatggcatt cgttcaattg cctaagtgtc tccagtaatt ttgcccttgc taaaatatgg
    51181 acctctggcg atacaataag tttactcgct gtttggataa actcatgata aggaagctgt
    51241 tcgatctcat caatagacgc aagctgacgt gcaggttcca acgaaggaag ttcctccgat
    51301 ttattgtgga gtgccttgat cttctgcctc tccatttgtc cggataattc ttctgcaaat
    51361 tggatttttt caatgacccg ctgctcgaaa gcttcataaa aatctgtact tgctatcaat
    51421 tttctagcat ataaaaaatc ttgtgtactg attaacaagt caaaataaaa ttcagcaaac
    51481 tcctcatttt gaagatactg tttttcatgc aagagtgctt gcttcaatgc ttcttttttc
    51541 tcgtccaact cgtaaagaca aaaaacaagc aggctattag cctcaaagct ttctttcaac
    51601 tcgtaagctc gttgaaataa ttcttttgcc tgctgatatt gctggtttgc tagtgcttgt
    51661 tgtgccactc gcattaaatt ctcatatttt tctggaaaat caatcggctt cacgcagtgt
    51721 cctcctcttg ctcatattca gctcatgttc aatagtagca aaacaaaaaa agcatgacaa
    51781 ttaaaaataa ctgaagcacc ctctaaaagc tagattttag atctgacttt tagaaggtgc
    51841 tttcaactgt tactgctttt tattgctcat cagctgttga cttcctggtt gaacgctttg
    51901 atcagattga tcgtaacaac acttgtatta gtgaaaaaat cgcccagatg ttgctttttc
    51961 tgattaaaga ctgctggaag ataccctaac atcagcaaag gaaattttag aatgaaccgg
    52021 caagcacctt ctctaactaa aacggttgac cagctaagtt cttcttcatc aaagctgatg
    52081 actcgaatac caaagatcat tttcccaatc gtttggccat gattgaattt cgtcaataaa
    52141 ataaaatacg ctagataaat aagtaaggct gtgaatccgt agagactcag ataagaatct
    52201 gaaaaattaa gccaacctag atttttggca ataccaagtg ttaaacgcgt aatagctccg
    52261 atacacaaaa gatcgaccaa gaaggcaaac attcggataa aaaaacctgc atagaaatac
    52321 ttaggaaaat catttgcttt tggcggttca tttcttgtgt ccgtatactt tttccattta
    52381 cgttgtttct ttttgatttc ttcgggcgtc aaaggctctc tttcaaaaga gctcagtacc
    52441 ttttgggaaa agttggagtc aggtcctaca gcaggctgct gttgcttatc tttttcttgt
    52501 tccttatttt catttgaaag ctctgacatg cttactcacc tccgtaataa tacattgctt
    52561 ttggtgattc cgctgtccct agattctcaa gaacggataa gatctgacta gtctcagaag
    52621 gcttcaatcc ctggaattct gctagtttac ttcctaacca tgtactagca aacccagatg
    52681 gcgtggaaga atattctact agttccgcat cttcaagctg ttgttccttt cgtatagctt
    52741 ttaaagcatc ttcaggaaat cccaattcat cgactaaacc tacttctttt gcttggacac
    52801 catcataaat ccttccatca gcaatttttt tgacttcttc ttcagattta ttccgtccct
    52861 cgctgacgat tgctacaaaa cgactatatg cgctgtcgat atatgcttga agcaccgctt
    52921 cttcttcttt tgtctctggt cgcgttcctg aacccatatc tttcaacgct ccgcttttaa
    52981 ctgttgtatc ttccacaccc agtttttcta gaagtcctga ataattcaat cctgacatga
    53041 tcacaccaat tgagccggtc actgtttctt ctgttgcaaa gattttatct gcttgagcag
    53101 aaatatagta gccaccgcta gctgccatgt ttttcatgct gacatacatt gggatatcat
    53161 gttctttacg aatcgtatcc aatttcttag cgatttccgc actttcgtag acaccgccac
    53221 caggagaatt tacttctaaa aagatacctt tgactgttgg atcttcttca atcgccttca
    53281 gttgttccat aaaattttta tgattgtaac cttcagaagc aaataaacct gaagaccctc
    53341 ccgcagcaat cgttccatct actgataatt ttacgatttt ttgcgtactg tctccctctt
    53401 ccaacacatt tggagctagt tcttcatccc catataacca actgttcaat ccctcaattt
    53461 gatcgttttc ctgaggctta gtcaaactag ctgaaactaa cgaaaccaca aataatcctg
    53521 cagcaatcag aactgctgcc caacgtcttt tattcattct ttctcctttc taatacaagt
    53581 tttttatgac gatcacagtt tctgactgct cagaagtgtc gaaccgctct gctttttctt
    53641 cccgcgcata cccggaaaac gactcttttg ttaattcatc ttctaccgta aactcacgag
    53701 cttgagcaac ttccaaggca tcaggtgcga tttcttggat gctatcccag ccagtatcta
    53761 aaaatatttt cgtatcatct gatacaccag ctgatgcttc gaatttggac aattgttttt
    53821 ttaacattcc cagtgtcatg atctcaattt ttgaatccat ttttactcct cctctatccg
    53881 attactttct attgtaccga actcctgaaa aagatgatag ataaaaaccc attatttttt
    53941 gacataaaaa ggacatttga caaagaattt gatatatacc aatttatttt ttattctttt
    54001 tttgttgaca agaacattcg caaaaggtta tgattttaaa tgtttccaga taaaaaacaa
    54061 aaaggaggat agttatacct tttaggatag cgccaggtct gtgtaaacag atgacgagga
    54121 gagggtttat cgaaagattc ggcggatggc cctagggact gcactctaca gaaaatccac
    54181 aaaaaatgtc tgcaaagata aaacaaaagg attttcctgc agctggaagc attcatacga
    54241 attcattata gaaagtagga tgttttttat gtgcggaatt gttggaatga ttggattaaa
    54301 aaatgtaaca ccaggattga ttgacggtct agaaaaatta gaataccgtg gttatgattc
    54361 agctggtatc tttgtctcag atggcacaac tgattatctg gtcaaagcac aaggcagaat
    54421 tcaaaatttg aaaaacaaaa taaatattga tactacagga aatataggta ttggtcatac
    54481 acgatgggca actcacgggc aaccttctga agaaaacgcc catccacata cttcccaaag
    54541 tggacgcttc gtgctcgttc acaacggcgt aatcgaaaac tttgaagaac ttaaaattgc
    54601 ttatttagca aatgaccact ttattggtga gaccgatact gagattatcg ctcatctaat
    54661 tgaaactttc gcgaaggata ctacaacgca agaggcgttt ttaaaagctt tgcgggtcat
    54721 caaaggttct tacgctttcg ctttgattga tcggacagca cctgatgtta tttacgtcgc
    54781 taaaaataaa agtccactat taatcggact cggcgatgga ttcaatgtga ttgccagcga
    54841 tgcaatggct atgcttgctc atacgaagga attcgttgag attgaagatg aagagatggt
    54901 taccgtcaca tcagaaaaag ttattattca aaattttgca ggtgacatcg tagaacgttc
    54961 ttcattcgaa gcacaagtag atgcttcaga tatcgagaaa ggtacctatc ctttttatat
    55021 gctgaaagaa atcgacgaac agcctattat catgcgtcgt atcgctcaaa aatacgtaac
    55081 agaagacaat cgtgtctctc ttgatcaaaa gctggtcgat acattgtctg atagcgatcg
    55141 aatctatatc gttgcttgcg gaactagtta ccatgcgggg ttagcaggta aacagacttt
    55201 ggaaaaattg acacagattc ctgttgaggt tcatttagcc agtgaatttg gctataacac
    55261 acctcttctt tcaaaaaaac cattctttat tttcttgagt caaagtggcg aaacagcaga
    55321 cagccgccaa gtgctggtaa aaatcaaccg tctcggctac ccttcactta cgattacaaa
    55381 tgttgctggt tctactcttt cgcgggaagc atcctttaca ctgctattgc atgctggacc
    55441 cgaaatcgca gtagcatcaa cgaaagctta tactgcacaa atagctgtac taactttatt
    55501 agccaaagct atcggtgata aaaaagaatt tgaaacttct cttgtattcg atatcactca
    55561 tgaattgagt ttagttgcta atgcgatgga atcggtcatc gcccaaaaag attatttaga
    55621 agagttggca gaagtatatc tcagcgatac aagaaatgcc ttttacattg gacgtggtgc
    55681 tgactacaac gtttctcttg aagctgcttt gaaattgaaa gaaatcagtt atatccaggc
    55741 agaaggtttt gccgctggcg aattgaaaca tggaacgatt gctttgatcg aagaaggaac
    55801 accagtgatc ggaatcatca gtgaagaagt gactggtgcg catactcgag gaaacctgaa
    55861 agaagtcgaa agccgtggcg ctaaaacact agttattgtc tcagagggat tagaaaagga
    55921 aagtgaccag ctggtgctac caactgtcca cccttatcta actactctaa ccacagtcgt
    55981 accgactcaa ttacttgcat attacgcaac gctattacgg ggattcgacg ttgacaaacc
    56041 acgtaattta gcgaaatcag tgacagtaga ataataaaag aacaaggacc agtgtcagac
    56101 atctgacact ggtccttgtt cttttatttt ttatgaaaat atctcttaat ccagtccgat
    56161 ttcttctttg actactgccg cgattttatc gacatagtaa ttaactttct catcagtagg
    56221 cgcttctgcc atcactcgaa gtaatggttc tgtaccggaa ggacgaacta ggattcttcc
    56281 ttctgatccc atttcagctt ctgcctcttc aatcactttt ttgattgccg gaacatccat
    56341 cgcaccgttt ttatcactta cgcggatatt cactaatttt tgcggataga ttgttacctc
    56401 tgctgcaagc tctgataatt tcttacctgt ctgtttcatg atattcagta actgaattcc
    56461 tgaaagcatc ccatctccag ttgtgttaaa atctaagaaa atcatgtgcc cagactgctc
    56521 gccgccaaag ttataatcgt ttttgcgcat ttcttctaca acgtaacgat caccaacttg
    56581 tgtaacgaca tctttcaaac cgattccttc caccgctttg tggaatccta agttactcat
    56641 gacagtcgta acgatcgtat cttttttcaa tcgatttttt tctgccaagt atttagcaca
    56701 gataaacatg attttatcac cgtcgacaat gtttccaagt tcatccaccg caataatacg
    56761 atcgccatcg ccatcgaaag ctagtccagc atctgctcct ttttcaacga ccatctcagc
    56821 taaacgttct ggatgggtag agcctacgcc gtcattgata tttaacccgt ctggatttgt
    56881 gcccattgta taaaaatctg tttcaagatc ggcaaacaaa cgattaacac ttgttgaagt
    56941 tgctccgttt gctgcatcga cacaaatggt caaaccggaa aggtcaccgt tgatcgtttg
    57001 tacaaggaat tgagaatatt tcaataagcc ttctggaaat tcttctacag ttcccaatcc
    57061 ttcagctgat ggacgaggca attcatccac ttcagcatct aataatgctt caatttctag
    57121 ctcttggtca tctactagtt taaaaccatc tgcaccgaaa aatttgattc cattatcttg
    57181 tgctggatta tgagaagcag aaatcatgac ccctgcgctt gctttttgca aacgtgtcag
    57241 ataagcaaca cctggtgtag agatcacacc tagctggaac acttcgatac ccaccgataa
    57301 aagtcctgaa atcagcgctt cttctaacat ttgacctgag attcttgtat cccgccctac
    57361 taacacgcgt gggcgttttc cttcttcttc atgctggctt aggacataac ctccgcaccg
    57421 tcccagctta aatgcaagtt ctggcgtaag ttccttgttg gcttcaccgc gtacgccgtc
    57481 tgttccaaaa tatttaccca tagttgtaaa attactcctt taacttaatt ttctgaggaa
    57541 tttgttctgc tcatttgtgt tgattcgctt gtttctgatg tttcttctgt acttgtggtc
    57601 gagctgcttg tcccagattc cgaactggaa gaaatggttg ttgtcgatgg ttccgttcct
    57661 gaatcagcag atgaaatact tctactcgta cttgtatttt cttgtgtccg actgctcgtc
    57721 gaagcagagc tactgcttga cgtttgcaac acgggtgtaa tctccacact aactgattcg
    57781 ggcttgatac ttaacccctc ttctactggt atttcaattt tacgagttgt tcgatgattg
    57841 atacctgaga cattgatcgg tacagcaata ctgtcaccga tcgatgcaag caaatcgcta
    57901 gagccgctta cttctgcttc cagttttgaa aaactgaaat catagctttc taccccggca
    57961 gctggtgttc cttgctgtat cccataaagt cgtaccgact tgcttggctt gatcgaatca
    58021 gctctcactg ttacttgcac tggatcagag acgatactta atacttcccc tgctgcattc
    58081 agcgcctgta ttgggacctc actattaatc ccatcatctg tgaccatcga agcgtccaca
    58141 tcagctacta ctcgtttgat ttctgccaat gttttgtcac ctgtagtgat agagactttt
    58201 ttaggactaa cggaagtatc atcaagttca tatccagatg gtgtgaacgt tgaaggaatc
    58261 aacgtttcca catcgaaatc ttttattacc tttttctcaa ccgttactgt aatagtcgat
    58321 ggttcgatct ttgctgtgac agcactgctc aaattttgaa tttttagcgg aacttcatgt
    58381 gttccttcgc ccagcttagt gatatctgct gtgacgcgga acatccgtgt atcttcgttt
    58441 gcctctgcat tcaactgaac tcggtttgca ctggaaagtt tgacagtcac tgcattttca
    58501 tatccatgga tatagtagcg ctttgaatca taagaaatct tgatcggcac ccccgtaact
    58561 gtttgattat acgcttcatt tccagaaagc gtatttggaa cggactggcc atttgcattg
    58621 aagtacagcg caatactgaa cagcaatgca ataaaactgt aaagaatgct tgtccgtttc
    58681 tcttttgaga tcatttgcga tcccctccct tagtcatacc gtctaggaaa gattgaatca
    58741 cgtttttctt gttttttgtt ttctcttcag caattagttc tctacgcaag attgccaaat
    58801 attcttgctg ggataagcca gccatcagtt cattatctaa cgtgatgctg acgtccccgg
    58861 tttcttcaga gacaacgatg gtaatcgcgt cactgacttc gcttataccg acagctgccc
    58921 ggtgtcgagt cccgaattct ttagggatca acatactttc tgatagagga agataggcgc
    58981 ttgctactgc gatctttcct tcttttacaa taactgcacc gtcatgcagc ggtgtattag
    59041 gaataaagat attgatcagc agctccccag tgatatctgc atctaaagca atccctgtct
    59101 caatatactc atctaatcca gtatgacgtt cgatagtgat caacgcaccg atttttcgct
    59161 tggacatata ttggatggct ttgtcaaaag acaagatcat cttctcatct tcctgctgtt
    59221 cgctttttga ttgtttgaaa agcgaactgc gtccaagatg ttccagtcct ctccggattt
    59281 ccggctgaaa aatcacgata gcagctatca ccccgtacgt aatgacttgg ttcatcagcc
    59341 aagacaaggt gtgcagacca atgatttccg ctacgattcg gataacaata aaaatagcta
    59401 cacctttcag aagctgaatc gcttttgtgc ctcgcagcag ttggatcagt ttgtatacaa
    59461 gataccagac aaccagaata tcaagtatgt tcaccaaaaa attcagagag aacaagtcac
    59521 cggaaaacat ttgctgccaa tagtccaaat taaaaagttc cccgatttga aatgacataa
    59581 aaagcctctc ttctcttttg agatttcctc tctagtatat catatgtttt aattttagag
    59641 gctgatatgc tcgtattttt cttgagaaaa acgcataatt ttcaagaaaa ttgcaagtat
    59701 gcgttttatt tttctctgca ataaatattt ttaaacttca gagaattatt cattatattt
    59761 tttctgttat ttttcctttt tttcttttgt agcttgttcc atttttttac gtcgttctgc
    59821 tctggcagct cttctagcag ctcttctttc ttctgcttca aggtccgtcg ttttttcttt
    59881 tctagtctta acgggtacat cgatagattc ctctggttca agtcgtttct tgattttttc
    59941 taattctccc gtcagtgcag catagttgta aaaacgtgtt ttagcgtcgt ggacagtctt
    60001 ttcaaataaa gcttttgcaa gatttggatt tgcgttatct agcgaggaaa agcgaacttg
    60061 tttcctcatg aaatcaggca tagcagagaa atcaggtttt ttgtagtcca aagtcattgg
    60121 gttcttgttt ttttcgacca ttgtagggtt atatcgatat aatgaccaat aaccagaaga
    60181 aacagcttct tttgcttctt taagcgattc tttcattccg ccaagcaagc catgtgtgat
    60241 acaaggcgta taggcaataa tcagagaagg accaggatac ttttctgctt cttcaaatgc
    60301 cttgatcgtt tgcatttgat tggcacctga agcaatttgc gccacataaa catttccata
    60361 tgtcatcgcc atcattccta gatctttctt tgaagcatac tttccactag ccgaaaattt
    60421 agcgattgcc gaagctggtg ttgcttttga ggtttgaccg ccagtattgg aatagacttc
    60481 gttatccaaa acaaggatat tcacgtctgc tccgctggca agtacatgat cgatcccgcc
    60541 aaaaccaatg tcgtaagccc atccgtctcc accaatcatc cattggctgt tttttacgaa
    60601 tagatccttt ctcttgtata tatcctccag ttcagggaaa attgatcgtt cgctttcgat
    60661 cgcagcttgc agttttgctg ctctttgctg cgttccttca ctctcaaaca tgtgtactaa
    60721 ccagtcttcc atcaattctc gtagttcttc tgatactttt ggcaatactt ctttgattga
    60781 acttgccaaa gcctcgcgac gagtctgtgc cgctagaagc ataccataac caaattcagc
    60841 attatcttcc aataaagaat ttgaccatgc aggcccttga ccacattcat tggtcgtata
    60901 aggagaagca ggagaagcac cgccccaaat agaagaacag cctgtcgcat tcgcgatcat
    60961 cattcgatca ccgaacattt gtgtcaataa ttttacataa ggtgtttcgc cacagcctga
    61021 gcaagcgccc gagaattcaa gaagcggttg attgaattgt gtagacaaga cagtattctt
    61081 tcctttgact ggatttgctt tttgtcttaa tgtcattgca aaagcccaat tcacagcttg
    61141 ttctttctgt tcttcataag gcttcatcac taaagctttt tcttttgcgg gacaagcctc
    61201 tacacacaga ccacaacctg tacagtcttc cactgaaact tggatacggt attttacgcc
    61261 gtctgcgcct cgcatttctc tcacgataaa tccttctggc gcttcttcca tttcctcttc
    61321 atctgctaaa aatggacgga tggcagcatg aggacaaaca aaggcacatt cattacacat
    61381 cgtacagcga tcactaatcc actcaggtac ttctaaagcg acgccccgct tttccataga
    61441 agccgttcct aaaggcattt cgccagttgt cattccattt gctacaagtg taccaacaga
    61501 gagttgattt ccttcctgtg cattcactgg ttcaagtatt tcttgtatat aaccagtgtt
    61561 ccttttattg attttttcag gcatctccaa cgttctccaa tggtctggga tcgcgacttg
    61621 atgaagcagt tcggtcgtcc gatcgattgc ttgtatgttt ttttctacga tagccattga
    61681 tttatgtcca tagcttttca gggcttcttc ttttagcagc ggaagcacct catcaaaagg
    61741 aatgatgtca gctaatttaa aaaaagccgt ttccattgcc gtattgatcc ggcgtcccaa
    61801 accgacttct tgggccaatc tcatggcatt gatcgtatag aatctaatgt tattttcagc
    61861 caagtaacgt tttaatttta agggcagatg cgtttctaat tgttcgtcgc tccaaagagt
    61921 attcaataaa aaaatgccac ctggcttcaa tcctttgacc aagtcatagc tatgcaggta
    61981 agctggtgtg tgacaggcaa caaggtccgc atgctcgaca agatacgctg aacgtatcgg
    62041 cgtatctcca aaacgaagat gagagacagt cagaccgcca gattttttcg aatcataata
    62101 aaaatagcct tgcgcatatt tatcggtgtg atctccaata atcttgatag ctgatttatt
    62161 tgctccaact gttccgtcag atccaaatcc ccaaaacttg gcctggaatg tttgcggttc
    62221 tgtcaaatcc agactttcct ttggttccaa agattgataa gtcacatcat ccacgatccc
    62281 tatggtaaac cgttttttgc gtatgcttgg gtcttttttc aattcatcaa aaactgctga
    62341 gatttggtcg ggagtcacat ctttagaccc caatccgtag cgaccaccaa taacagccgg
    62401 gcgaagttca gaatcatata acgcactttg aacgtctaat aagagaggtt ctccacctgc
    62461 ccctggttct ttacttcgat caagaacagc aattgttttt actgtatgag gcaacttttg
    62521 cacgaatgtt tccactggaa aaggacgata aagatggata ttcaaaaaac ctgttttccg
    62581 tccgtttgca ttcaagtaat caatcgtttg ttcaatcgtc tgtgcaactg atcccataga
    62641 aacaatgact tcttccgcat cgtctgctcc gtaataagtc accaagtcat aagaagttcc
    62701 tcgtaatcgg ttgatctctt tcatatattt ttgaacgatt tcaggtaatt tatcataata
    62761 ggggttgatc gtttcccgtt gttgaaaatg gatgtctgga ttctggttcg taccactaac
    62821 agtcggatga ttgggattca tccctcgttt acgaaatgaa gctaatttct cctgattgat
    62881 cagaggagct aattcttcat attccaaaac ttcgattttt tgaatctcgt gactcgtacg
    62941 gaatccatca aagaaattca aaaaagggac ttctccttca atagaagcaa gatgggcaac
    63001 aggtgctaaa tccatcactt cttgtacact gctttcagct aacatagcaa atcctgtttg
    63061 tcttgcagcc atcacgtctc cgtgatctcc gaaaatattc aacgcatttg tcgtcactgc
    63121 ccgactagca acgtggaaaa cacttggcag tagttcccca gcaatcttgt acatattcgg
    63181 tatcatcaaa agcagccctt gtgaagcggt atatgtagtt gtcagtgcac cagttttcaa
    63241 tgatccatgg accacaccag ctgctcctgc ttcggattgc atttctacta cttttactgg
    63301 ttgaccgaat acgttttctt ttcctgctgc tgaccattga tctactaatt cagccatggt
    63361 cgaactgggt gttattggat atattgcagc aatctcggta aatgcataag aaatatatgc
    63421 ggctgccgta ttgccatcca ttgttttcat ttttcgcatg attcatcatc cttgttcata
    63481 ttctctctta ttatctgttc cattataacg taaaacatgc catttttttc ttttatttat
    63541 aaaaatattt tacatgtatc tatttgaatt ggtcatctaa tttttagaca aacagtctaa
    63601 atcgttgcct ttttttgtca gacatgtata aactataagc gaaagaggtg ttttaacatg
    63661 gatatatcag tgattgatgc aacgaaaaca aatacacaaa aaggaatcct ttatggttca
    63721 tcgaatgctc ctaaaaaaat ggtcgaattc atcaacttag cttgtccgta ttgccgacaa
    63781 tggtttgaag aatcttatga gctattagaa gaagcagtcc aaagcgggca attgcagcga
    63841 gtgatcaaat tgttcgataa agaaaaagaa agcttacagc gagggaacgt gatgcaccgg
    63901 tatctcacaa tcagcgatgg ccaaaaagcg atcaaagaga tcaaacaaat attcgatact
    63961 caagacgagt ggaaacatct ttctctccaa gaagtagcgg attttgcagt agataaacta
    64021 aagctgacag aacaaaaaga cgaacagctg agccaagctg tcatcaacga agctgagcaa
    64081 gcacatattc gatttgttcc aactgtcatc ttgggtaaag aaatctttga tgaatctatt
    64141 tcaatagagg aattaaaaga attgatccaa acaaaataaa aaaataagga gcgtg
//
LOCUS       DAFFFQ010000006        62731 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000006
VERSION     DAFFFQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.006"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            125..1168
                     /locus_tag="LQB69_000473"
     CDS             125..1168
                     /locus_tag="LQB69_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL3524663.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="LQB69_000474"
     CDS             complement(1230..1694)
                     /locus_tag="LQB69_000474"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL3524664.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="LQB69_000475"
     CDS             1940..2887
                     /locus_tag="LQB69_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3524665.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="LQB69_000476"
     CDS             complement(3098..5170)
                     /locus_tag="LQB69_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3524666.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="LQB69_000477"
     CDS             complement(5103..5480)
                     /locus_tag="LQB69_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL3524667.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6187)
                     /locus_tag="LQB69_000478"
     CDS             complement(5609..6187)
                     /locus_tag="LQB69_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3524668.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="LQB69_000479"
     CDS             6319..7611
                     /locus_tag="LQB69_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3524669.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="LQB69_000480"
     CDS             complement(7671..8789)
                     /locus_tag="LQB69_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL3524670.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LQB69_000481"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="LQB69_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL3524671.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LQB69_000482"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="LQB69_000482"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL3524672.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="LQB69_000483"
     CDS             11995..12594
                     /locus_tag="LQB69_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL3524673.1"
                     /translation="MNYFISDMHFFHEHLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LQB69_000484"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="LQB69_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL3524674.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="LQB69_000485"
     CDS             complement(14289..14633)
                     /locus_tag="LQB69_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL3524675.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15915)
                     /locus_tag="LQB69_000486"
     CDS             complement(14770..15915)
                     /locus_tag="LQB69_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3524676.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="LQB69_000487"
     CDS             complement(16095..17069)
                     /locus_tag="LQB69_000487"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL3524677.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="LQB69_000488"
     CDS             complement(17149..18024)
                     /locus_tag="LQB69_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL3524678.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="LQB69_000489"
     CDS             18154..20061
                     /locus_tag="LQB69_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL3524679.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="LQB69_000490"
     CDS             complement(20602..23880)
                     /locus_tag="LQB69_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL3524680.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="LQB69_000491"
     CDS             complement(24151..24696)
                     /locus_tag="LQB69_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3524681.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="LQB69_000492"
     CDS             complement(24708..25400)
                     /locus_tag="LQB69_000492"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL3524682.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="LQB69_000493"
     CDS             complement(25419..25703)
                     /locus_tag="LQB69_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524683.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="LQB69_000494"
     CDS             complement(25716..26279)
                     /locus_tag="LQB69_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3524684.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="LQB69_000495"
     CDS             26507..27169
                     /locus_tag="LQB69_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL3524685.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="LQB69_000496"
     CDS             27293..28489
                     /locus_tag="LQB69_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL3524686.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="LQB69_000497"
     CDS             complement(28590..29966)
                     /locus_tag="LQB69_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3524687.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="LQB69_000498"
     CDS             complement(30003..30869)
                     /locus_tag="LQB69_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL3524688.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="LQB69_000499"
     CDS             complement(30958..31983)
                     /locus_tag="LQB69_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3524689.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="LQB69_000500"
     CDS             complement(32001..32675)
                     /locus_tag="LQB69_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3524690.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="LQB69_000501"
     CDS             complement(32859..33752)
                     /locus_tag="LQB69_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3524691.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="LQB69_000502"
     CDS             complement(33826..35523)
                     /locus_tag="LQB69_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3524692.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="LQB69_000503"
     CDS             complement(35516..36829)
                     /locus_tag="LQB69_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL3524693.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="LQB69_000504"
     CDS             complement(36829..40347)
                     /locus_tag="LQB69_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL3524694.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="LQB69_000505"
     CDS             complement(40640..41098)
                     /locus_tag="LQB69_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3524695.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(41396..41914)
                     /locus_tag="LQB69_000506"
     CDS             complement(41396..41914)
                     /locus_tag="LQB69_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL3524696.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(41916..42533)
                     /locus_tag="LQB69_000507"
     CDS             complement(41916..42533)
                     /locus_tag="LQB69_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL3524697.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSFYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42530..43384)
                     /locus_tag="LQB69_000508"
     CDS             complement(42530..43384)
                     /locus_tag="LQB69_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3524698.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43381..44292)
                     /locus_tag="LQB69_000509"
     CDS             complement(43381..44292)
                     /locus_tag="LQB69_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524699.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44333..45667)
                     /locus_tag="LQB69_000510"
     CDS             complement(44333..45667)
                     /locus_tag="LQB69_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3524700.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            45875..46705
                     /locus_tag="LQB69_000511"
     CDS             45875..46705
                     /locus_tag="LQB69_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL3524701.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(46949..47267)
                     /locus_tag="LQB69_000512"
                     /pseudo
     CDS             complement(46949..47267)
                     /locus_tag="LQB69_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(47389..47583)
                     /locus_tag="LQB69_000513"
     CDS             complement(47389..47583)
                     /locus_tag="LQB69_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524702.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(48088..48273)
                     /locus_tag="LQB69_000514"
     CDS             complement(48088..48273)
                     /locus_tag="LQB69_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524703.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(49123..49464)
                     /locus_tag="LQB69_000515"
     CDS             complement(49123..49464)
                     /locus_tag="LQB69_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3524704.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(49464..49694)
                     /locus_tag="LQB69_000516"
     CDS             complement(49464..49694)
                     /locus_tag="LQB69_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3524705.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            49981..50319
                     /locus_tag="LQB69_000517"
     CDS             49981..50319
                     /locus_tag="LQB69_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524706.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(50524..50763)
                     /locus_tag="LQB69_000518"
     CDS             complement(50524..50763)
                     /locus_tag="LQB69_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL3524707.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(50739..51086)
                     /locus_tag="LQB69_000519"
     CDS             complement(50739..51086)
                     /locus_tag="LQB69_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL3524708.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(51818..52399)
                     /locus_tag="LQB69_000520"
     CDS             complement(51818..52399)
                     /locus_tag="LQB69_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3524709.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLVKERLLTSQKVL"
     gene            complement(52582..53208)
                     /locus_tag="LQB69_000521"
     CDS             complement(52582..53208)
                     /locus_tag="LQB69_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3524710.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(53230..54561)
                     /locus_tag="LQB69_000522"
     CDS             complement(53230..54561)
                     /locus_tag="LQB69_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL3524711.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(54716..55117)
                     /locus_tag="LQB69_000523"
     CDS             complement(54716..55117)
                     /locus_tag="LQB69_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL3524712.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVFEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(55203..55409)
                     /locus_tag="LQB69_000524"
     CDS             complement(55203..55409)
                     /locus_tag="LQB69_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524713.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(55504..57006)
                     /locus_tag="LQB69_000525"
     CDS             complement(55504..57006)
                     /locus_tag="LQB69_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL3524714.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(57024..57479)
                     /gene="argR"
                     /locus_tag="LQB69_000526"
     CDS             complement(57024..57479)
                     /gene="argR"
                     /locus_tag="LQB69_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL3524715.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(57577..58194)
                     /locus_tag="LQB69_000527"
     CDS             complement(57577..58194)
                     /locus_tag="LQB69_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL3524716.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            58415..59788
                     /locus_tag="LQB69_000528"
     CDS             58415..59788
                     /locus_tag="LQB69_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3524717.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            59863..60975
                     /locus_tag="LQB69_000529"
     CDS             59863..60975
                     /locus_tag="LQB69_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL3524718.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(61047..62684)
                     /locus_tag="LQB69_000530"
     CDS             complement(61047..62684)
                     /locus_tag="LQB69_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL3524719.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT        17939 a        13304 c        11084 g        20404 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgtcaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttattatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacacttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctagccaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct ttcctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattata atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    41281 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    41341 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    41401 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    41461 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    41521 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    41581 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    41641 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    41701 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    41761 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    41821 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    41881 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    41941 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    42001 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    42061 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    42121 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    42181 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    42241 atctgataat cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    42301 taaaaagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    42361 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    42421 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    42481 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    42541 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    42601 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    42661 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    42721 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    42781 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    42841 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    42901 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    42961 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    43021 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    43081 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    43141 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    43201 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    43261 atgaagtaaa caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga
    43321 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    43381 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    43441 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    43501 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    43561 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    43621 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    43681 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    43741 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    43801 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    43861 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    43921 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    43981 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    44041 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    44101 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    44161 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    44221 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    44281 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    44341 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    44401 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    44461 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    44521 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    44581 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    44641 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    44701 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    44761 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    44821 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    44881 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    44941 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    45001 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    45061 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    45121 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    45181 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    45241 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    45301 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    45361 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    45421 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    45481 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    45541 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    45601 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    45661 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    45721 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    45781 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    45841 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    45901 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    45961 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    46021 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    46081 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    46141 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    46201 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    46261 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    46321 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    46381 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    46441 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    46501 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    46561 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    46621 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    46681 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    46741 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    46801 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    46861 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    46921 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    46981 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    47041 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    47101 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    47161 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    47221 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    47281 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    47341 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    47401 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    47461 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    47521 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    47581 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    47641 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    47701 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    47761 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    47821 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    47881 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    47941 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    48001 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    48061 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    48121 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    48181 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    48241 gatggttagg aatcctttag aatttttact cattcataga cacttcctta aataactttt
    48301 attatagaag gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaaac
    48361 ttacattata ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga
    48421 tcgtgtaatc ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa
    48481 ctaatcataa aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca
    48541 ctatctgacc caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta
    48601 aataaaaact agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt
    48661 gatggcaaat ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt
    48721 cagttaaact atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga
    48781 gcatcgatat agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact
    48841 aaaactgtca gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata
    48901 cgttaagata tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga
    48961 aatcacctca atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta
    49021 atgccatcga ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt
    49081 atgtttagca tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat
    49141 aatctgatca attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg
    49201 ttccttaaaa tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc
    49261 ttcaggtaat gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac
    49321 cgaaaacatt gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt
    49381 ttgaatttct tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg
    49441 aatataaatt ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc
    49501 cattcgtctt tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt
    49561 ggaactaacg taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa
    49621 ggtttctttc cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat
    49681 tttgtaacaa gcattatcat cactccctta ataaaagtat actatataac actaagtaac
    49741 gcaaaattcc cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg
    49801 atataaatag aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa
    49861 gaaacattga acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc
    49921 ttcaattttg cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc
    49981 atgcccgatt ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga
    50041 aagttcacga aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt
    50101 caaatagaaa cattggttca acgaatttca actagacaag actttgctat tgtagaacgt
    50161 gagatcgtag ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg
    50221 gataaatggc aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta
    50281 actttgaatt ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata
    50341 aataaaaaag actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg
    50401 actgtgaccc tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt
    50461 tttgggatct gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt
    50521 atgttatacc ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa
    50581 agaaatatct gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc
    50641 atggattggt gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt
    50701 ttcatcctga tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa
    50761 cataaatttg tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat
    50821 ggttgatgaa aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt
    50881 ccttttttaa atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc
    50941 tcttttctta acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct
    51001 gcctgcttcg gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct
    51061 agatcaaaaa aataacgacc acacatccta atccctccca atcttttttt ctatcataca
    51121 ctgcaacaag aagttcgcac caacaaaaca ctttcaattt ttgtagaact aaaaaagttg
    51181 tgacaagtga caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc
    51241 acaatattca acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga
    51301 aacggcaggc ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag
    51361 ccaaatttat gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac
    51421 taagcgactt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    51481 taagcccaac caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg
    51541 atgcagggca gctttttcac aacctctcca ctaaacgatg ttcaatcagc taaccttcgg
    51601 tattagctca aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact
    51661 caggtcaaag cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg
    51721 aaaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    51781 tcttgaagct gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta
    51841 acaatctttc tttgaccaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt
    51901 tcaacaataa gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt
    51961 cttcatcgga cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc
    52021 attgattgac taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa
    52081 taatcaaatt acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa
    52141 tccagtccaa catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt
    52201 cgaacaattc tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt
    52261 tttttgaaga aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat
    52321 ctcggattga cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga
    52381 tcttttcctt tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac
    52441 agtaaattca tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac
    52501 acagccctca ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg
    52561 aggacaaaag caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac
    52621 ttgatctgca ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc
    52681 accaattgca tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg
    52741 ggaatcgcta cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt
    52801 tctcaattgg agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat
    52861 gatcgtattt tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc
    52921 ttccactgat tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc
    52981 tcctgcaccg ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa
    53041 gagtttgtat cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt
    53101 gtatttttct aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc
    53161 gacttgcggg tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc
    53221 attcgttatt tattttaata catcatttag agcttcactc attgtcggat gagaatagat
    53281 ttgatctctc aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat
    53341 gacttcatga gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc
    53401 gactaaaact ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct
    53461 tgtcatatac ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa
    53521 acctacattg gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt
    53581 cgttgttcgt ttattctcgc ccttcaactg atccataatg attcgataat catctagaga
    53641 gatataagtg aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc
    53701 tgtacgaagg tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt
    53761 ttctaatcca agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc
    53821 ttggattgtt acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc
    53881 tgctacttga tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc
    53941 taaaatcatt tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag
    54001 aacgacaact ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc
    54061 agccccgatg atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc
    54121 aatcaagtag cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt
    54181 attgatgaaa atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat
    54241 ttgatggtta gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct
    54301 gatcatatga taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc
    54361 ctctgtaaag tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc
    54421 gatgttgata catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc
    54481 accttttgaa gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat
    54541 attttgatat ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa
    54601 aatatgataa ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac
    54661 cagacaaaaa gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa
    54721 caatcgttac ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat
    54781 gcgcatcttc tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcga
    54841 atactacacc gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta
    54901 ctgtggactc tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac
    54961 aagaagcata acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg
    55021 gaggagtgat tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc
    55081 catggttgat cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc
    55141 atttgctact tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct
    55201 gcttattcga ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta
    55261 ctcgtgaaca acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct
    55321 tttatatgat tgattttaag atacatatat accagaaagt tgaccccata aaccaatact
    55381 gttaaaaaaa taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag
    55441 cactcgattt aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact
    55501 ggtttattct gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt
    55561 tactaacgcc aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg
    55621 ctcatgtaag cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata
    55681 tcgaatacct tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg
    55741 cttcatttct tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa
    55801 tcggatttct tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat
    55861 accttttctt tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc
    55921 atccagaaaa tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt
    55981 atctttccca agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat
    56041 ataacgaact tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg
    56101 ggaattcgtc acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc
    56161 gatcaaatta tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac
    56221 atctgtatcg aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact
    56281 ttccatcgct tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc
    56341 caaaaattgg gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa
    56401 tccaccagca ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc
    56461 tagtgttcca atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag
    56521 gtctgctttt tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat
    56581 aaaagcaata tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata
    56641 ataataggga agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa
    56701 ttgagtggac ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc
    56761 ttgttctttc agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc
    56821 atgaccaatc ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat
    56881 ccagtttgct agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata
    56941 tccttcaact ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc
    57001 tttcatataa aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc
    57061 tgtgcttctt cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg
    57121 acaacttcag ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt
    57181 tcagtcaata cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt
    57241 gctgtacgca aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt
    57301 actattttgt tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc
    57361 gttgcttgag tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc
    57421 ccaataggtt gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata
    57481 ttagacacct ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt
    57541 attttttact tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg
    57601 caaaatcgtc ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt
    57661 gtgttcttta gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa
    57721 cgttgctggt tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc
    57781 catctttttg aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat
    57841 ggcttcttgt gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga
    57901 aaaatgttct tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc
    57961 ttccagtgga tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga
    58021 catgcgtatt gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg
    58081 attcgggtcg ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg
    58141 cgggcttttt ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct
    58201 tcctccgcga aattccattt agttatttat tatcatacct ctttttgagt gacttggcta
    58261 atcgtgttga cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag
    58321 ataacacgtt tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc
    58381 tcttttttta cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt
    58441 agtacctgca gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc
    58501 catgaatgta actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca
    58561 atgggtaaca acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt
    58621 gaatcgcaat tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg
    58681 tataatgatc gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca
    58741 aggtgcagcg acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc
    58801 cctcaatcga cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc
    58861 aattggcccc acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct
    58921 gtttcttgtg cccgttttga ttctttcttt gattatcggc tggtttgcta ttcccgagat
    58981 ggcagtacaa aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt
    59041 tagtggaagt ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat
    59101 catcggcggt attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact
    59161 gatccgactg tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt
    59221 ttatcagttt ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca
    59281 aggaagtagt ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt
    59341 gctttctcct ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc
    59401 tgggctgatt ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc
    59461 agtgggtgta ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag
    59521 caatatgatg accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc
    59581 aatgttcaat accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat
    59641 tatcaattac gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc
    59701 aaaagcagca ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta
    59761 ttatctgttc tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt
    59821 ttttttgtgt gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt
    59881 atcggtggcg ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat
    59941 gttgctttat ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc
    60001 ccttggttgt cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct
    60061 tttttcccta aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg
    60121 acacttttgc ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga
    60181 aaagaagaag cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa
    60241 gcacttccgt tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat
    60301 ggaaaagcat atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc
    60361 caggcaaaag tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga
    60421 gaatacacag ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa
    60481 gaattaggct atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca
    60541 tcttataaga tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt
    60601 cctttcatgg atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat
    60661 ttggctccta ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact
    60721 gatccagatc agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg
    60781 tcagattttt ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc
    60841 ggtctaggct cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc
    60901 ttaaatactg gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg
    60961 aaaaatccat catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg
    61021 gattacttct tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt
    61081 tcttttgctt catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga
    61141 atatcaagtc gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa
    61201 tctggtatag gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt
    61261 tgttgcacga ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg
    61321 aaatatccag tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa
    61381 gcttgatagt cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact
    61441 agctcatcta gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat
    61501 gttgcaccaa aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa
    61561 tcatctttca tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg
    61621 ggtgtcgcat aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact
    61681 aggacttttt ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa
    61741 gaaatggccg gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag
    61801 gcagtagcaa aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca
    61861 gagtgacctt ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca
    61921 aatacagcca tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag
    61981 gcaataaagc cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat
    62041 tctccaaagt aacgtaatcc tagcacaacc gctgtattat ggagagcatc aaacaaaatg
    62101 agtccatttg gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc
    62161 tcatcaaagg aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa
    62221 ttcagcaaat aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc
    62281 acagaaaatt cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga
    62341 ctttgataca cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc
    62401 tcaaagatcc gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt
    62461 gtcctaacat cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg
    62521 atcgttcctg gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga
    62581 acattattac cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt
    62641 ccacttgtga tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc
    62701 ctaaacaaaa aaacgttttc tttattatac a
//
LOCUS       DAFFFQ010000007        62295 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000007
VERSION     DAFFFQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.007"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            83..970
                     /locus_tag="LQB69_000531"
     CDS             83..970
                     /locus_tag="LQB69_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL3524720.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="LQB69_000532"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="LQB69_000532"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL3524721.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="LQB69_000533"
     CDS             2272..2718
                     /locus_tag="LQB69_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3524722.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="LQB69_000534"
     CDS             2991..4343
                     /locus_tag="LQB69_000534"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL3524723.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="LQB69_000535"
     CDS             4514..4801
                     /locus_tag="LQB69_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524724.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="LQB69_000536"
     CDS             5027..5470
                     /locus_tag="LQB69_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3524725.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="LQB69_000537"
     CDS             complement(5476..6660)
                     /locus_tag="LQB69_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL3524726.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="LQB69_000538"
     CDS             6928..7626
                     /locus_tag="LQB69_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL3524727.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="LQB69_000539"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="LQB69_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL3524728.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="LQB69_000540"
     CDS             complement(8436..8969)
                     /locus_tag="LQB69_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524729.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="LQB69_000541"
     CDS             complement(9266..11647)
                     /locus_tag="LQB69_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL3524730.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LQB69_000542"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="LQB69_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL3524731.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="LQB69_000543"
     CDS             12348..12887
                     /locus_tag="LQB69_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL3524732.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="LQB69_000544"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="LQB69_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL3524733.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="LQB69_000545"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LQB69_000545"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="LQB69_000546"
     CDS             13994..15160
                     /locus_tag="LQB69_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL3524734.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="LQB69_000547"
     CDS             15206..16702
                     /locus_tag="LQB69_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL3524735.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="LQB69_000548"
     CDS             16783..17466
                     /locus_tag="LQB69_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3524736.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="LQB69_000549"
     CDS             18111..19301
                     /locus_tag="LQB69_000549"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL3524737.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="LQB69_000550"
     CDS             19403..20356
                     /locus_tag="LQB69_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL3524738.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="LQB69_000551"
     CDS             20425..21774
                     /locus_tag="LQB69_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3524739.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="LQB69_000552"
     CDS             complement(21957..22280)
                     /locus_tag="LQB69_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524740.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="LQB69_000553"
     CDS             22409..23572
                     /locus_tag="LQB69_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL3524741.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="LQB69_000554"
     CDS             23642..24991
                     /locus_tag="LQB69_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3524742.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="LQB69_000555"
     CDS             complement(25036..25467)
                     /locus_tag="LQB69_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3524743.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="LQB69_000556"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="LQB69_000556"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL3524744.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="LQB69_000557"
     CDS             28697..29293
                     /locus_tag="LQB69_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3524745.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="LQB69_000558"
     CDS             29415..30563
                     /locus_tag="LQB69_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL3524746.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="LQB69_000559"
     CDS             complement(30601..31014)
                     /locus_tag="LQB69_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL3524747.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="LQB69_000560"
     CDS             31093..31524
                     /locus_tag="LQB69_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL3524748.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="LQB69_000561"
     CDS             complement(31623..31856)
                     /locus_tag="LQB69_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL3524749.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHQ"
     gene            32039..33709
                     /locus_tag="LQB69_000562"
     CDS             32039..33709
                     /locus_tag="LQB69_000562"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL3524750.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="LQB69_000563"
     CDS             33856..34242
                     /locus_tag="LQB69_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524751.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="LQB69_000564"
     CDS             34229..34927
                     /locus_tag="LQB69_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3524752.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="LQB69_000565"
     CDS             34932..35396
                     /locus_tag="LQB69_000565"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL3524753.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="LQB69_000566"
     CDS             35393..36304
                     /locus_tag="LQB69_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3524754.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="LQB69_000567"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="LQB69_000567"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL3524755.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="LQB69_000568"
     CDS             37092..38375
                     /locus_tag="LQB69_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL3524756.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="LQB69_000569"
     CDS             38388..39314
                     /locus_tag="LQB69_000569"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL3524757.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="LQB69_000570"
     CDS             39333..40613
                     /locus_tag="LQB69_000570"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL3524758.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="LQB69_000571"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="LQB69_000571"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL3524759.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="LQB69_000572"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="LQB69_000572"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL3524760.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="LQB69_000573"
     CDS             44938..45714
                     /locus_tag="LQB69_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL3524761.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="LQB69_000574"
     CDS             45711..46643
                     /locus_tag="LQB69_000574"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL3524762.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="LQB69_000575"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="LQB69_000575"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL3524763.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="LQB69_000576"
     CDS             47363..47992
                     /locus_tag="LQB69_000576"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL3524764.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="LQB69_000577"
     CDS             47980..48294
                     /locus_tag="LQB69_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524765.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="LQB69_000578"
     CDS             48332..48973
                     /locus_tag="LQB69_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3524766.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49016..49708
                     /locus_tag="LQB69_000579"
     CDS             49016..49708
                     /locus_tag="LQB69_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL3524767.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="LQB69_000580"
     CDS             49705..50607
                     /locus_tag="LQB69_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3524768.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LQB69_000581"
     CDS             complement(50765..52471)
                     /gene="efbA"
                     /locus_tag="LQB69_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL3524769.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="LQB69_000582"
     CDS             52661..53284
                     /locus_tag="LQB69_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524770.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="LQB69_000583"
     CDS             53734..54783
                     /locus_tag="LQB69_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3524771.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="LQB69_000584"
     CDS             54780..55661
                     /locus_tag="LQB69_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524772.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="LQB69_000585"
     CDS             55664..56434
                     /locus_tag="LQB69_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3524773.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="LQB69_000586"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="LQB69_000586"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL3524774.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="LQB69_000587"
     CDS             57745..58908
                     /locus_tag="LQB69_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3524775.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="LQB69_000588"
     CDS             59025..59435
                     /locus_tag="LQB69_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3524776.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="LQB69_000589"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="LQB69_000589"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3524777.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62008..62292
                     /locus_tag="LQB69_000590"
     CDS             62008..62292
                     /locus_tag="LQB69_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524778.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT        20574 a        10800 c        13382 g        17539 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattcccct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattgat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg atttgactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaa
//
LOCUS       DAFFFQ010000008        59612 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000008
VERSION     DAFFFQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.008"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(451..1761)
                     /locus_tag="LQB69_000591"
     CDS             complement(451..1761)
                     /locus_tag="LQB69_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL3524779.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LQB69_000592"
     CDS             complement(1773..3191)
                     /locus_tag="LQB69_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3524780.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="LQB69_000593"
     CDS             complement(3431..4999)
                     /locus_tag="LQB69_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL3524781.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="LQB69_000594"
     CDS             complement(5003..5413)
                     /locus_tag="LQB69_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL3524782.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="LQB69_000595"
     CDS             complement(5410..6333)
                     /locus_tag="LQB69_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL3524783.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="LQB69_000596"
     CDS             complement(6330..7208)
                     /locus_tag="LQB69_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL3524784.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="LQB69_000597"
     CDS             complement(7195..8532)
                     /locus_tag="LQB69_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL3524785.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="LQB69_000598"
     CDS             complement(8535..9638)
                     /locus_tag="LQB69_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3524786.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="LQB69_000599"
     CDS             complement(9712..11220)
                     /locus_tag="LQB69_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3524787.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="LQB69_000600"
     CDS             complement(11186..11878)
                     /locus_tag="LQB69_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL3524788.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="LQB69_000601"
     CDS             complement(11900..12874)
                     /locus_tag="LQB69_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524789.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="LQB69_000602"
     CDS             13174..14046
                     /locus_tag="LQB69_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3524790.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="LQB69_000603"
     CDS             complement(14504..16702)
                     /locus_tag="LQB69_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3524791.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="LQB69_000604"
     CDS             16934..17926
                     /locus_tag="LQB69_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524792.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="LQB69_000605"
     CDS             17986..19407
                     /locus_tag="LQB69_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL3524793.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="LQB69_000606"
     CDS             19400..20899
                     /locus_tag="LQB69_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL3524794.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="LQB69_000607"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="LQB69_000607"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL3524795.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="LQB69_000608"
     CDS             22491..23174
                     /locus_tag="LQB69_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL3524796.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="LQB69_000609"
     CDS             23176..24012
                     /locus_tag="LQB69_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL3524797.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="LQB69_000610"
     CDS             24023..25777
                     /locus_tag="LQB69_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524798.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="LQB69_000611"
     CDS             complement(26053..26394)
                     /locus_tag="LQB69_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3524799.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="LQB69_000612"
     CDS             complement(26462..28633)
                     /locus_tag="LQB69_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL3524800.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGATETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="LQB69_000613"
     CDS             28802..29701
                     /locus_tag="LQB69_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3524801.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="LQB69_000614"
     CDS             complement(29772..30629)
                     /locus_tag="LQB69_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3524802.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="LQB69_000615"
     CDS             complement(30614..33316)
                     /locus_tag="LQB69_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL3524803.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="LQB69_000616"
     CDS             complement(33329..34618)
                     /locus_tag="LQB69_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL3524804.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="LQB69_000617"
     CDS             34768..35814
                     /locus_tag="LQB69_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524805.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="LQB69_000618"
     CDS             35816..37969
                     /locus_tag="LQB69_000618"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL3524806.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="LQB69_000619"
     CDS             complement(38442..39893)
                     /locus_tag="LQB69_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3524807.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="LQB69_000620"
     CDS             complement(39890..41614)
                     /locus_tag="LQB69_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3524808.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="LQB69_000621"
     CDS             complement(41607..42221)
                     /locus_tag="LQB69_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL3524809.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="LQB69_000622"
     CDS             complement(42566..44023)
                     /locus_tag="LQB69_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3524810.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="LQB69_000623"
     CDS             complement(44064..44984)
                     /locus_tag="LQB69_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3524811.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="LQB69_000624"
     CDS             complement(44996..45943)
                     /locus_tag="LQB69_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524812.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="LQB69_000625"
     CDS             complement(46422..47141)
                     /locus_tag="LQB69_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL3524813.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="LQB69_000626"
     CDS             complement(47190..48836)
                     /locus_tag="LQB69_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3524814.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="LQB69_000627"
     CDS             49015..49605
                     /locus_tag="LQB69_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3524815.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="LQB69_000628"
     CDS             49697..51202
                     /locus_tag="LQB69_000628"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL3524816.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="LQB69_000629"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="LQB69_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL3524817.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="LQB69_000630"
     CDS             complement(52638..53156)
                     /locus_tag="LQB69_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524818.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="LQB69_000631"
     CDS             complement(53172..53498)
                     /locus_tag="LQB69_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL3524819.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="LQB69_000632"
     CDS             complement(53511..55490)
                     /locus_tag="LQB69_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3524820.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="LQB69_000633"
     CDS             complement(55511..55825)
                     /locus_tag="LQB69_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL3524821.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="LQB69_000634"
     CDS             55993..56286
                     /locus_tag="LQB69_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL3524822.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="LQB69_000635"
     CDS             complement(56364..57476)
                     /locus_tag="LQB69_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3524823.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..57853
                     /locus_tag="LQB69_000636"
     CDS             57629..57853
                     /locus_tag="LQB69_000636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524824.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            57863..58042
                     /locus_tag="LQB69_000637"
     CDS             57863..58042
                     /locus_tag="LQB69_000637"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524825.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="LQB69_000638"
     CDS             58236..58406
                     /locus_tag="LQB69_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3524826.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..59570)
                     /locus_tag="LQB69_000639"
     CDS             complement(58830..59570)
                     /locus_tag="LQB69_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3524827.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17820 a        12182 c        10422 g        19188 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggc tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    59461 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    59521 atctttgtca acacgacacc gcctggtgta agtcggtttg ctttcgtcaa ttctaccacc
    59581 gctgtctgac ttctttcgat ctcaaactcg at
//
LOCUS       DAFFFQ010000009        56347 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000009
VERSION     DAFFFQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.009"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            290..1135
                     /locus_tag="LQB69_000640"
     CDS             290..1135
                     /locus_tag="LQB69_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3524828.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="LQB69_000641"
     CDS             1416..2636
                     /locus_tag="LQB69_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL3524829.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="LQB69_000642"
     CDS             2633..5353
                     /locus_tag="LQB69_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3524830.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="LQB69_000643"
     CDS             5337..6281
                     /locus_tag="LQB69_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3524831.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="LQB69_000644"
     CDS             complement(6350..7360)
                     /locus_tag="LQB69_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3524832.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="LQB69_000645"
     CDS             complement(7540..7884)
                     /locus_tag="LQB69_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL3524833.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="LQB69_000646"
     CDS             complement(7877..8311)
                     /locus_tag="LQB69_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL3524834.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="LQB69_000647"
     CDS             8449..9186
                     /locus_tag="LQB69_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3524835.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="LQB69_000648"
     CDS             9179..10390
                     /locus_tag="LQB69_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3524836.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="LQB69_000649"
     CDS             10594..11376
                     /locus_tag="LQB69_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL3524837.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="LQB69_000650"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="LQB69_000650"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL3524838.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LQB69_000651"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="LQB69_000651"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL3524839.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="LQB69_000652"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="LQB69_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL3524840.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="LQB69_000653"
     CDS             14978..16027
                     /locus_tag="LQB69_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL3524841.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="LQB69_000654"
     CDS             16024..16302
                     /locus_tag="LQB69_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL3524842.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="LQB69_000655"
     CDS             16277..16747
                     /locus_tag="LQB69_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL3524843.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="LQB69_000656"
     CDS             16764..17066
                     /locus_tag="LQB69_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524844.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="LQB69_000657"
     CDS             17083..17454
                     /locus_tag="LQB69_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL3524845.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="LQB69_000658"
     CDS             17426..17785
                     /locus_tag="LQB69_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524846.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="LQB69_000659"
     CDS             17957..18964
                     /locus_tag="LQB69_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3524847.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="LQB69_000660"
     CDS             18989..20173
                     /locus_tag="LQB69_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL3524848.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTMMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="LQB69_000661"
     CDS             complement(20610..22193)
                     /locus_tag="LQB69_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3524849.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="LQB69_000662"
     CDS             complement(22771..22914)
                     /locus_tag="LQB69_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL3524850.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23137..23616)
                     /locus_tag="LQB69_000663"
     CDS             complement(23137..23616)
                     /locus_tag="LQB69_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3524851.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23689..23973)
                     /locus_tag="LQB69_000664"
     CDS             complement(23689..23973)
                     /locus_tag="LQB69_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524852.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24051..24245)
                     /gene="ssrS"
                     /locus_tag="LQB69_000665"
     ncRNA           complement(24051..24245)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LQB69_000665"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24317..25603)
                     /locus_tag="LQB69_000666"
     CDS             complement(24317..25603)
                     /locus_tag="LQB69_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL3524853.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKSAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25741..26412
                     /locus_tag="LQB69_000667"
     CDS             25741..26412
                     /locus_tag="LQB69_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL3524854.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26378..26866
                     /locus_tag="LQB69_000668"
     CDS             26378..26866
                     /locus_tag="LQB69_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL3524855.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26879..27826
                     /gene="prmA"
                     /locus_tag="LQB69_000669"
     CDS             26879..27826
                     /gene="prmA"
                     /locus_tag="LQB69_000669"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL3524856.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27827..28591
                     /locus_tag="LQB69_000670"
     CDS             27827..28591
                     /locus_tag="LQB69_000670"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL3524857.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28824..31037
                     /locus_tag="LQB69_000671"
     CDS             28824..31037
                     /locus_tag="LQB69_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL3524858.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31048..31494
                     /locus_tag="LQB69_000672"
     CDS             31048..31494
                     /locus_tag="LQB69_000672"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL3524859.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31665..32924
                     /locus_tag="LQB69_000673"
     CDS             31665..32924
                     /locus_tag="LQB69_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL3524860.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33013..33960)
                     /locus_tag="LQB69_000674"
     CDS             complement(33013..33960)
                     /locus_tag="LQB69_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3524861.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34354..35646
                     /locus_tag="LQB69_000675"
     CDS             34354..35646
                     /locus_tag="LQB69_000675"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL3524862.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35661..37430
                     /gene="aspS"
                     /locus_tag="LQB69_000676"
     CDS             35661..37430
                     /gene="aspS"
                     /locus_tag="LQB69_000676"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL3524863.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37539..38414
                     /locus_tag="LQB69_000677"
     CDS             37539..38414
                     /locus_tag="LQB69_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3524864.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38680..40563
                     /locus_tag="LQB69_000678"
     CDS             38680..40563
                     /locus_tag="LQB69_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL3524865.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40638..40922)
                     /locus_tag="LQB69_000679"
     CDS             complement(40638..40922)
                     /locus_tag="LQB69_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL3524866.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40912..41646)
                     /locus_tag="LQB69_000680"
     CDS             complement(40912..41646)
                     /locus_tag="LQB69_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL3524867.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41775..42398
                     /locus_tag="LQB69_000681"
     CDS             41775..42398
                     /locus_tag="LQB69_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL3524868.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42590..43711
                     /locus_tag="LQB69_000682"
     CDS             42590..43711
                     /locus_tag="LQB69_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL3524869.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43718..46846
                     /locus_tag="LQB69_000683"
     CDS             43718..46846
                     /locus_tag="LQB69_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL3524870.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46865..50059
                     /locus_tag="LQB69_000684"
     CDS             46865..50059
                     /locus_tag="LQB69_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL3524871.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50205..50912)
                     /locus_tag="LQB69_000685"
     CDS             complement(50205..50912)
                     /locus_tag="LQB69_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL3524872.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51677..52075
                     /gene="spxA"
                     /locus_tag="LQB69_000686"
     CDS             51677..52075
                     /gene="spxA"
                     /locus_tag="LQB69_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL3524873.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52407..53060
                     /locus_tag="LQB69_000687"
     CDS             52407..53060
                     /locus_tag="LQB69_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL3524874.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53286..54284
                     /locus_tag="LQB69_000688"
     CDS             53286..54284
                     /locus_tag="LQB69_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL3524875.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54460..56268
                     /gene="pepF"
                     /locus_tag="LQB69_000689"
     CDS             54460..56268
                     /gene="pepF"
                     /locus_tag="LQB69_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL3524876.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18538 a         9724 c        11679 g        16406 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatcccaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggttt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cggaccagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg agactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaatgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acggtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatac cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccatggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttta ttttacttga actcattgac tgttttccct ttattttaaa
    23041 cattataaag accgatgagt gcttgccgcg tcttttgttt ttatcacggt tttttgtgca
    23101 aaaaaacctg ccgatagttg gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc
    23161 tggcaattgg ttttctaaat cagttcggac aacaagcaca tcgcaagctg cgtttctaat
    23221 aacatattca gaaacagaac caataaataa acgttctaca gcattcaatc ctgtggctcc
    23281 taacataatc aaatctactt gattatcttc tgggatttct ctggcaataa tttgttttgg
    23341 tgagccatat tctacgacag aggttacatt attcaagcca gctttcttcg cattgctttc
    23401 ataatctgcc aatgtttgct ttgccatttc agtagcttgt tctgcaagca ttccatcaaa
    23461 ggatgaaacg gtttggaaag aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt
    23521 atttcggata gctacattga ccgccttttt aaaagctaat tcagcttcat cagaaccatc
    23581 tacggcaacc atgatttttt tgtattgttg taacataagc attcactcct caagtcaatc
    23641 gtattgtact cttttattgt agaacacttt agggaaaaag agaattattt actgctttgg
    23701 ttcagcaatt ttttttgcaa atcggatact gaaggtggca gaaacaataa ttacgatcag
    23761 taaaaagacc aaggcaataa agcgttgatt gaaaaaagct gttaaaatac ccattacgcc
    23821 taaaacagca tatgcagctc cgtaaaattg taggaatcct tgatttttat catttttgtt
    23881 gatcaataca aaaaagatat cctgtttatt aaataaataa tagctaacga ataataatac
    23941 taaagtggcg ataatcatta aaatcctaat catgaacaaa tcctccaaaa taagcaacaa
    24001 atagaaaaag caatttgcca agagcaaatt gctttattct ttaaaagatg gtaatctccg
    24061 ttaatgccgt tgtttgtcct gtagtattga tcccgcgaat tcaagcaggt gggtcccatg
    24121 gtcgtagctt cgaactacca aatgataagg catgttacca gcagtgacac aagacgggtc
    24181 ccaagatatc aataaaaatg ttcggtcaac acacaaagag gacaattcga acatcacaga
    24241 tttcccacct ctatagtagc atatgaattt aaaaagagca atgagagagc tcgcagaaac
    24301 agaaccgttt tcttgtctag ttttttggag gatgcttttt ccactctttt atacggtaat
    24361 actgttgacc cagagcctgt tcatatttcc ctgtatcttt tggctgataa tactgagcac
    24421 tcttcagttt atctggtaaa tattgctgat tgacccaagc ctgatcaaaa tgatgaggat
    24481 actgataacc cacgccacgg ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat
    24541 gatcaggaac atctccagct ttgccctctc gaatatcagc aagtgctgca tctaatgcca
    24601 tgtaggcaga attggatttt ggcgacaagc aaagatcgac gacgacgtct gctagtggga
    24661 tacgtgcctc aggcaatccc aatttttcag cagctaatac agcgttcact gtacgagccg
    24721 cagcagcagg attacctaaa cctatatctt cataaccgat aaccatcaga cgccggcaaa
    24781 tacttgccag atcacctgcc tcaactaaac gggcgagata atgaagtgca gcatccacat
    24841 cacttccacg aatcgatttt tgaaaagccg aaataacgtc ataatgagca tctccatttt
    24901 tgtcatgggt aagagctttt ctttggacac attcttcaat gatcgacagt gtaagatgaa
    24961 tccttccttc tttatctgaa ggagtcgata gtgtagccag ttccagccca ttcaatgcac
    25021 tccgcaaatc gccgttcgtt gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga
    25081 ttgcttgttg tccaaggccg cgttctttat cccttaaagc atgttcgact gccaactgga
    25141 tgtcttgttc tgttaatggt ttgacttcaa agatctgtgt acggctgcgg atcgctggat
    25201 tgatggtaat ataaggattc tcagtagtcg ctccgatcaa gatgattcgg ccactttcta
    25261 aatgaggaag cagaaaatcc tgttttgttt tatctaatcg gtgtacttca tccaataaga
    25321 ggatcactgt accactcatt tttgcttctt ccgctacaac ttgtagatcc ttttttgaat
    25381 ctgtagcggc gtttagcatg cgaaatgcat aattagtaga gccggcgatc gcacttgcga
    25441 tactcgtttt tccagtacca ggaggtccat atagaatcat ggaagagagc atgcgagcat
    25501 cgaccatgcg gcgtatgatt tttccttccc cgactagatg ctgctgacca acgacttcat
    25561 cgatcgttcg aggtctcatg cgataagcca atggctgttg cataggattt tcctccttct
    25621 tgtttttacg tcccttaatt atatcatatt tagcaagatg cctagaaaga acagccttct
    25681 gttgttttgt tttcatagct tatccaacta atattgtgta tgataaagta ggtgagaaaa
    25741 atgacggata aacaaaagat agatgaattt ttttggaata aaacgacgcc agaagtggca
    25801 cgggatttat tggggatgta tttggagcat gacacaccag aaggaagatt agctggatat
    25861 attgtagacg cggaagcata cctaggtcca gaagacgagg ccgcccatag ctatggttta
    25921 agaagaacac cgcgtgtgcg ggcaatgtat gaaaaaccag gtacgatcta cctttacacg
    25981 atgcatactc atcggatttt gaacatcatt actcagccag aagggattcc tcaaggagta
    26041 atgatacgtg caatcgaacc cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga
    26101 aaaactggtc cagatatcag caacggtccg ggaaaattag tggaagcatt agcaattcct
    26161 caagaacttt atggtcagtc tatagcagat agttcgttac gcttagtttt tgagaaaaaa
    26221 aagacaccaa aaaagatcaa tgctttgcct agaatcggta tcccaaacaa aggtgtatgg
    26281 acagaaaagc cgttgcgttt tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa
    26341 aaccaaatcg agaaagattg gggttggagg aaagaaaatg aaaaagaagg atctattaac
    26401 atatttagat gaaataattg aaaaacaagt gacagactat gatgtagctt tagattggga
    26461 tacaaaaaat catacgatcg agatcgttgt tcgcttgttt gcagaaaata aagcacaatt
    26521 tgagattgat gatgcagaag ggatcgtttc gcaagaacca attatcgaat ttgaagacgg
    26581 cgtacttctt tttaatcctc agaaatctgt ttttgatgaa cgcaattatc tagcagtaat
    26641 cccttatgaa ggaaaaaaag ggttggcaaa atcagtagct gattctttag tggaatatct
    26701 aaatgaagta ttagctcaag ggcaaagcga tttgcttgat tttttggatg aagacgatga
    26761 cgaagctgta ttcgagcttc actgggataa tcagcaacta gcagaattag tagaagtaaa
    26821 acagcaaaat gaagcagata cttatttacc gtacccaagt tattaaggag tgacagatat
    26881 gaaatggaat gaagtaaaag tagaaactga aagtgaagca gtcgaagcag ttgctaatat
    26941 tttgatggag gcaggggcga gtggtgtcgc gattgaagat tctttagatg ttgaacattt
    27001 tcagtcggat caatatggtg agattttaga taaaaatgat tttacatcgc tgaaagaagg
    27061 cgcactagtc atggcttatt ttccggaaac gactttctta ccggaaatct tgccttttat
    27121 caaagatcgt atcacacaat tacctgagtt tggtctagct attggaaaaa ataaagtaac
    27181 agttagtgaa gtagaggaaa gcaactgggc aacagcctgg aagaaatact atcatccggt
    27241 gcgtattacg cgaatgctga cggttgcgcc aagctgggaa aactaccaga cagaagatcc
    27301 tctagaaaaa attatcacac tagatcctgg tatggcattt ggcacaggca ctcatcccac
    27361 gacttccttg acattgacag cactagaaac agtcttgcgt ggtggtgaaa cattgctaga
    27421 tgtgggaact gggtcgggcg tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt
    27481 ctatgccttt gatttagacg aagtagccgt tcgttcggcg aaagaaaata tggatatgaa
    27541 tgaaatagcc aaagatgtcc atgtatcagc taacgatctt ttgacaggaa tcgagattga
    27601 aagtgatgta atcgttgcga acattttagc agatatcatc ttattgatga tcccagatgc
    27661 ttggcgttta ctgaagcaaa caggcacgct gatcgtttca ggaatcattg aagagaagaa
    27721 gcaaatggtt ctggatgcca tgttagaaca agggtttatc gtggatcaaa ttttccagca
    27781 aaaagactgg tatgcaatta ttttgaaaaa accagaggtg gaatagatgc agcggtattt
    27841 tttaaaagaa tcatatgaat caaaagacta ttttgtttta gaaggggaaa actatcacca
    27901 cattgtacgg gttatgagga tggaacccga ggatgaagtt tttttggcat tcaatgatca
    27961 aatagccatt gttgcagaaa tcacggagat tactgaagag gctgtttatc tgaaagaaat
    28021 taaaaaagaa gaaaatcaaa aggaactgcc aagttcgatt accatagcga gtggctatcc
    28081 taaaggggaa aagctggatt ggatcgttca gaaatccact gaattaggag cgcaagcttt
    28141 tcttggtttc ccggcatcag cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg
    28201 acaacaacgg cttgaaaaga ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt
    28261 gccatcagta gagttattgg aaacgaaaca agatttattt gagcggctag atgattatga
    28321 cgtggtcttg gttgcttatg aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa
    28381 aattcttagt gaaacaaaac cagatagtcg aatcttagct gtctttggac cagaaggtgg
    28441 attatcccgc gatgaagtcg attattttgt agaacacaaa gggatacttt gtggattagg
    28501 accaagaatc cttcgtacag aaacagcacc tctttattta ttaagtgctg ctagctatca
    28561 atgggaatta atgaatcctt ctttcagcta acgttcttca ttaataaaag agtatcgaca
    28621 gaaagaattg aaaaaatagt caacaatttg tataatagaa acaattattt atacaatgga
    28681 tacgaaaaat cgtttgaaaa agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa
    28741 ctgcacggat tttgggacaa ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa
    28801 aaaagaaagg gggatcgatt cctgtgacaa aagaagaaat tttaacggga cccggtgtca
    28861 taaagctcgt tagtcaatac atgggtcccg aacatgtagc atttgttgag aaagcatgcg
    28921 aatacgcgac tgctgcacat gacggacagt ttagaaaatc aggcgaaccc tacattattc
    28981 atcctatcca agtcgcaggt atattagcag atttaaaaat ggatccccat acagtggcta
    29041 caggcttctt acatgatgtt gttgaagata cagaaatcac tttagaagat ctgagagaag
    29101 aatttggcga tgacgttgct atgttagtgg acggcgtaac caaattaggg aaaataaaat
    29161 ataaatccca cgaagagcag ctagcagaaa accaccgaaa gatgctgcta gcaatggctc
    29221 aagatttacg agttatcatg gtcaaattag ctgatagact acataacatg cgtacgttga
    29281 agcacctgcg agaagataag caaagaagga tcgctcagga aactttagaa atctatgcgc
    29341 ctcttgcaca tcgtctaggg atcagccgga tcaaatggga attagaagat acagcacttc
    29401 gttatctaaa tccaaaacag tattaccgta tcgtccactt gatgcagacg aagagagaag
    29461 aacgtgaaaa atacgtaagt ggtactgttg aagatattcg aatagcgaca gaagagctgg
    29521 ggatttttgc agaaatctat ggacggccaa aacacattta ttcaatctat cgtaaaatga
    29581 aagatcagaa aaagcagttc aacgaaattt atgacttgct agctattcga gtgatcgtag
    29641 attcaatcaa ggattgttat gctgtattag gagcaatcca cacaaaatgg aaaccaatgc
    29701 ccggcagatt taaggattat attgccatgc caaaagctaa tatgtaccaa tctttgcata
    29761 ctaccgtgat cggtccggca ggaaatccgg tagaaattca aatccgaaca caggaaatgc
    29821 atgaaatcgc tgaattcggg gttgctgcac actgggccta taaagaagga aaaaatgaaa
    29881 aagtagaacc agatggtatg acgaaacaat taagctggtt ccatgagata ctcgaacttc
    29941 aagacgaaag ctatgatgct tctgaattta tggaaggcgt aaaaggagat atctttagtg
    30001 ataaagtcta cgtcttcaca ccaaaaggag acgttactga gttaccaaaa ggatccggac
    30061 cattggactt tgcatacagt atccatacag atatcggtaa caaaaccact ggtgcaaaag
    30121 taaatggcaa aatggtgcag cttgattaca aattgaaaaa cggagatatc attgagatca
    30181 tgacttctcc aaattcattt ggcccaagtc gcgactggtt gaaattagtt gctactagca
    30241 aagcaagaaa taagatcaaa cgtttcttca aagcccaaga tcgagaagaa aatgtgatca
    30301 aaggccacga atccgtggtc aaatgtatta cagatctagg atttacgcct aaagatattt
    30361 tgacgaagaa caaactgcaa gaagcactcg atcgttttaa ttatcaaaca gaagatgatc
    30421 tctatgcagc tgtagggtat ggagaagtta gccccttgac gatggccaat cgtctgactg
    30481 aaaaagaacg taaagaacaa aaaatcgagc agcaaaagca agaagcagaa gaaatcatga
    30541 atcagccgaa aaaagaacct gacaaaatga aagtacgtca tgaaggtggc gttgtcattc
    30601 aaggggtaga aaacttacta attcgtatca gtcgctgctg taatccgatt cctggtgatg
    30661 atatcgttgg ttatatcact aaaggcagag ggatatccat tcatcgtcga gattgtccga
    30721 atgttcagcc tgacaaacca aatgtagcag aacgtttgat tgaagtcgaa tgggaagata
    30781 catcgaatac acgaaaagag tatgatgcag atttggaaat ttacggctat aatcgttcag
    30841 gcttattgaa tgatgtactt caaacagtca atgcgctaac gaaaaatctc aacagcgttg
    30901 aagcacggac gaataaagat aaaatggcga cgatccattt gacggttggt atccagaatt
    30961 tatcccacct aaagagtatc gtggataaaa tcaaagcagt acctgatgtc tacagtgtac
    31021 gccggacgaa tggataggag gacagatatg agagcagttg ttcaacgggt aagtaaagct
    31081 gctgtgtcag tcgatcagca agaaattgga aaaattggct acggcctact tgttttatta
    31141 ggcattcatg aaaccgatac gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa
    31201 atgcgtattt ttgaagatga acaaggaaaa atgaatatta gtgtagaaga tgtgggagga
    31261 caaattttaa gtatctctca attcacactg tttgcggata cgaaaaaagg aaatcgacca
    31321 agctttgtgg aagccgcacg accagaaaca gctattcctt tatacgaatc attcaatgaa
    31381 ggaatcagaa gtcgcggcat cacagtcgct accggacaat tcggagcaga tatgtcggtt
    31441 tctctcgtca atgatggacc tgtaacgatc attattgatt ctaaaaacaa ataaagtaat
    31501 ctggttttca ctagtaaaaa ctatgaaaca cgtcgcttaa gttagactga taaactctaa
    31561 cttaagtggc ttttttgtct gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt
    31621 aagcttaaat tatacaagtt attttttata ggaggttgct gaagatgaaa gaagtaccag
    31681 tagtagatat caaaatcaca gatttaaaaa aattgtatca aaaaggatac aacatgttgg
    31741 aggagttacg ccatgaagcg gacgctcccg tcgtcaaagc aaagattttc aataaagaag
    31801 ctatcacgat ctacggatca tcagcagcaa aagtatttta tgatccaagg aatttcaaac
    31861 gaaaaggtgc tatgccaaaa ttagtattga agacactttt tggtcaaggt ggagtacaga
    31921 ctttggatgg agctgctcat caccatcgga aaaatatttt tatggatcta atgacaccgg
    31981 aaagaatgga agattatcat cgtatcttag ataagaatct gacacaagca cttgaagcac
    32041 agcatggtca atttgaacta tttgatttaa gtaagatggt tttctttaca tctatttgcg
    32101 agtgggcagg tatcaattta tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat
    32161 atcaaatctc tatgatcagc ggaacgttta cttctccgat tgatcacata aaaggtgtcg
    32221 aaaatcgcaa aaaatcagaa aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc
    32281 ctgtggctgg aaaagaaaat gtggcattat atgcttttgc caatgccaca gatttagatg
    32341 gtcagctttt acctctggaa gtggcagctg ttgaattatt gaacatcatc cgtccaacgg
    32401 ttgctctaac tgtgtgggcg gcattgatgg gacatgcact tttcagtaga ccagatttgt
    32461 atcagcaatt aaaaaatgac ttttcgacgt tacaagatcc gtttattcaa gaaatgagac
    32521 gctattatcc tttcttccca atgctacctg cgatttcgct gaaagaagta gaagtagatg
    32581 gttatcgtat accagaaggc agttgggtta ttttagattt atatggaaca gaccatgatg
    32641 aacgaacagt tgaagcgcca gattccttta tgatcaaacg gtatgtaggg aaagcaaaag
    32701 atatttccta taaagaagaa tatgagatga ttgcacaagg cgggggaaat tttcgacaaa
    32761 tgcatcgctg tgcaggagaa tggatcactc ttcatagtct acgtgtgttt tccgatcagc
    32821 tagtaaataa gtttgagttc tctgtacctg aacaagattg gacgattcca ttcaatcagt
    32881 ttccgactta tccgaatagc cgagcgctac tttacaagaa ttagattagc ataaacttat
    32941 cgaaaaaaag gaagatcaaa cagtgctgat cttccttttt tcatattact ataaattgat
    33001 aaggtcaatt gatcaaaatc cacgaaatgc ttcatcaatg gtttgataat cttcattgga
    33061 taaatgcaaa tctaaagctt ggacattgtc agatacttgc tctggacgct tagctccagg
    33121 tataactaca gacactcgtg gattcttcat gtaccaagct aaaacaagtt gcgcaggagt
    33181 ggcttggtat cttttagcaa tggggcgaag tacatcgaca gcggttaaaa tttctctgaa
    33241 acgttctcct tggaaatctg gatttctttt tcgaggatct ccttcaccaa attgtttttc
    33301 ctcaccaagt tcatattttc ctgtcagcaa accggaagca agaggaaaat agggaacaaa
    33361 actgattttg tttttttcaa gataagggaa taattctttt tcagcctgcc gatggatcaa
    33421 actgtattta tcttctacta catcgacata tccatctgca ttcgcttctt tcaattgttc
    33481 taacgtgaag tttgacacac cgactgctcg gatttttcct gcttctttta attcgtgcaa
    33541 tgtagcaact gattcatttt ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc
    33601 gatgtaatca gtttgcagac gttttagtgc atcttccacc gattgtttga gaaactcagg
    33661 agaattatca aatgttcgcc ctttatttgg tacatgagca gcttttgtag cgatgacgac
    33721 acgtgagcga tcatattctt tttccttcag tacctcgcca atcagttctt cggaacggcc
    33781 gtttccgtaa gcataagcgg tatcgatcaa attgatcccg ctattcaaag ctgtacgaac
    33841 tacttgtttt cctgtctcat cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt
    33901 tgctcctaat ccaagctgtt ctgcgtagac ttgtgaatgt ccgattctta tttctgccat
    33961 agctgattcc tcctttttgt ctagtctaaa acaaaatacc aaaaagctga actaaaacgt
    34021 ttttgtgagg ggaaatagtc agatatctct tgactttgag actggttttt agtaggttat
    34081 aagtaatatc gaatgtcaat gaaagagcga gtaagttttt gaaactttac aagagaggat
    34141 catcgaggct gaaaatgatc taaagtttca ttagcctaaa gacactctgg agatggacga
    34201 gtgaaaagcc gtcccgcgta cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat
    34261 taggtggtac cgtgtatgaa tttcattcac ccttgttcat gatgaacaag ggtgattttt
    34321 ttatttacat agctaagaaa gggagagaaa actatgagtt tccaaagacc aaaaggaaca
    34381 aatgatattt tgccaggcga atcagaaaaa tggcagtttg tcgaagaaac agcacgttta
    34441 ttattcagag attatcagta taatgaaatg cgtacaccga tttttgaaca ctatgaagtg
    34501 atatcccgaa gtgtagggga tacaacggat atcgtttcaa aagagatgta tgatttttat
    34561 gacaaagggg aacgccatgt gacgcttcga ccagaaggta cagcaccaat cgttcgttct
    34621 tttgttgagc ataaattgtt cggcccagag tatgcgaaac cgttcaaaac tttttatatg
    34681 ggaccaatgt ttcgctacga acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc
    34741 ggagtagaag catttggatc agaaaaccct gcaactgatg tggagagcat ggtgatggca
    34801 cttgatttct tcaaacagtt ggggatcacg caaatccggc tagtcatcaa ttcattaggt
    34861 gataaagaaa cgcgtcaagc ctatcgtcaa gcattgatcg actatctttt accttttgaa
    34921 tcagagctga gtgaagactc taaacgccgc cttcatgaaa atccattacg cgtattggac
    34981 agtaaagaca aacgagatca aaaatttgtg gcagaagctc catctatctt agattatcta
    35041 agcgagcctg ctcaaaaaca ttttgatacc gttgtttcca tgctggatgc attggctgtt
    35101 ccttatgaaa tcgacagtaa tatggttcgc ggactagatt attacacaca tacgattttt
    35161 gaaatcatga gtgacgcacc aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt
    35221 tatgacaatc tagtggaaga actaggtggt ccttcaacac ctggcttcgg atttgcaatg
    35281 ggtatcgagc gattactctt gactatggag gcagaggaag tagttatccc tgcatttaat
    35341 gagttggatg cttacgtggt cgcactcgga gacgaaacaa atatcgaagc attgaaagtg
    35401 gtccaagcga ttcgaaactt tggtttttca gctgaccgcg acttcatgaa ccgtaaagca
    35461 aaagcgcaat ttaagacagc agataaagcg aatgccaaat tagtattggt gatcggtgaa
    35521 gatgagcttg ctagtcaaac gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt
    35581 tttccattag aagagatata tgaacatttt gatgaagtct atgatgaaat gactttattc
    35641 gagtaggagg aaaaaaataa atggcacaaa gaacgatcta ttgcggatta gtttccgaac
    35701 aacaattaga acaaacagtt acattaaaag gatgggtaca aaaaagacga gaccttgggg
    35761 gagtaatctt tatcgaccta cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg
    35821 aaatcaacaa agaagcatgg gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag
    35881 tgactgggga agttagaaga cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg
    35941 aattcgaagt gattgcaagt gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc
    36001 aaattgaaga tgatcaagca atcagtgacg aaatacgttt gaaatatcgt tatttggatc
    36061 tgcgtcgtcc aaaaatgact aaaaacatca aattaagaaa tgcgacaaca aaagcgattc
    36121 gtcattattt agatgataat gactttctcg atatcgaaac accttaccta gggaaatcta
    36181 caccagaagg cgcaagagac tacctagttc cttcacgtgt ccatgctgga catttctatg
    36241 cgttgccgca atctcctcaa ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt
    36301 attatcaaat cgttcgttgt ttccgcgatg aagatttacg tggagaccgt cagcctgaat
    36361 ttacgcaagt agatattgaa acaacgttct taacagcaga agaaatccaa acttacacag
    36421 agggattgat tgcgaaggta atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc
    36481 ctcgtatgac ctatgatgaa gcaatggctc gctacggcag cgacaaaccg gatacacgtt
    36541 ttgatatgga attgatcgat ctaagcgata cagtcaaaga agtagaattc aaagtcttcc
    36601 aaatggctct tgaaaacggc ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc
    36661 gctactcacg aaaagacatg gatcagttag gtcaatatgt aggacaattc ggagctaagg
    36721 ggcttgcatg gctgaaagta gaagaagacg gattgaaagg tccaattgct aaattcatgg
    36781 gagaagcaac agaagcaatc attaaagcaa cagatgcaaa accaggtgac ttgctgatgt
    36841 ttggcgcaga taaaagtgaa atcgtcgctg ctgcattagg tgctatccgg acacgtttag
    36901 gaaaagaatt aggactaatt gatgaatcga aattcaattt cttatgggta actgattggc
    36961 cacaatttga attttcagaa gaagaaggtc gatatgtctc tgctcaccat ccattcacga
    37021 tgccaaaaga agaagatatc ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag
    37081 cttatgatat cgtcttgaac ggttatgaac taggcggagg ttctatccgt attcatactc
    37141 gtgagcttca agaaaaagta ctagaaactc taggctttac aaaagaacaa atggaagaac
    37201 aatttggttt cttgttaaca gctttagatt acggattccc tccacatggc ggtattgcat
    37261 taggattgga ccgttttgca atgttattag ccggagaaga taatattcgt gaagtcattg
    37321 cctttcctaa aaacggaaaa gcagcagatg tgatgagtga tgcaccaagt atggtttcgc
    37381 ctttacaatt gttcgaattg aatattgatg taacgaatat agaagaatag ccgataaaaa
    37441 ggtaaaaagg atttgtcgaa tacgcttctt ttgacaacct tgatttaatt gaaaaacact
    37501 ttataatgaa gccataacat ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt
    37561 gggcttatca tgattacacg accaagcttt cggtatctat cgtttatgcg ttgatcgcat
    37621 cagtcgcatt gaatttcttt tatcagccag gacatattta ttcaagtggt attactggtc
    37681 tagctcagat tttgacgact ttgtcaaaat cactgatagg attcgaagta ccggtatcaa
    37741 caactttgta tttactgaat atacctttgt ttgtattggc gtggttaaaa atcgggaaga
    37801 agtttacaat tttcactttt atcacagtca ctttgacctc cctttgtatc catattttac
    37861 cggaaacagt cctatctacc gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg
    37921 gactaggtat tgggctagcg ttgagaaacg gcctatcatc gggcggtcta gatatcgtga
    37981 gtatcacgat tcgtaaaaaa acaggaaagt cgattggttc tatttcgatc tacttcaatg
    38041 cattgatcgt ttttgttgcc ggaattctat ttggctggca atatatgttt tacagtacgt
    38101 tgtctatttt cgtcagtgga aaagtaactg atgtcgttta tactaaacaa aagaagatgc
    38161 aggtaatgat cgtaacgaaa catccagaaa aagtagtaaa tgaaatccaa aactgtatgc
    38221 gtagagggat cacgatcatc aatgaagcag aaggtgctta taagcatgat aagcagacag
    38281 tattgatgac gatcgttaca cgatacgaac tgcctctact tagagaagca atgaaagctt
    38341 ctgatccaca agcttttgta agtattgcag aaaacgtcca gatacttggt cgtttctatg
    38401 aggatggcat ttagatcgag tttgacaaaa attcatgaaa tactgctgta gacagacgtt
    38461 cgtttgtctg cagttttttt ctaccatagt agaatcagca aagaaaaact gcttttttat
    38521 ttgaaatttt cttaaagttg aaaaaaagca tgtttatttt tttaaattta tgggatgata
    38581 gaaaaaaata gtagagaaag gacacgattc tacttgctat tttcacttaa tacccgtata
    38641 ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta
    38701 aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt ggtgggtgtt
    38761 atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt tccgattttt
    38821 ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga acaaaatgga
    38881 aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc ggcacttgtt
    38941 tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct gttaaacaat
    39001 cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca aatcaataac
    39061 ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat ctggtcttta
    39121 gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt gatggtattt
    39181 gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct ctccgccatc
    39241 ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta cggtaccgac
    39301 actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt
    39361 ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg aggaatctcg
    39421 cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt catctattac
    39481 ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac agcacacccc
    39541 ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg caaacgaagc
    39601 tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa ggtgactgat
    39661 attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct aatcatcagt
    39721 gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc
    39781 caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc atgggttgtt
    39841 ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc gaaaaacgct
    39901 gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa attagcagaa
    39961 gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat ccttgataag
    40021 tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac ggcatttggt
    40081 gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa agtagtggta
    40141 gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa attaaaggaa
    40201 caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc attctcggaa
    40261 gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc
    40321 cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa
    40381 aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca tagtgactgg
    40441 ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat tcacttagtc
    40501 agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa cgtgacagaa
    40561 tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt tttttatatt
    40621 taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa
    40681 taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg
    40741 cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt ttgaagacga
    40801 gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag atcggttgta
    40861 tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg ttcatttgcc
    40921 atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag tgatcaatgg
    40981 tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta acaaaatatt
    41041 tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc
    41101 ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag taaagcgtcc
    41161 atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg tatgaatctc
    41221 atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca gccctttgaa
    41281 ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag ataagctgtc
    41341 tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg cgatactacg
    41401 tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg cctgggttcg
    41461 tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt
    41521 tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag aaaacacttt
    41581 ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg
    41641 aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta tttttattaa
    41701 aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac cataagaagg
    41761 gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta ctattagcct
    41821 tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat gagaattaca
    41881 tactagttgc tccccatcgt acttggtggg acccattata tctggcggta gctgcagcac
    41941 ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt ctgcgattta
    42001 tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca agcgtcatta
    42061 aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt cccagtggga
    42121 caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg tctaaagcga
    42181 aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc aagcgtcaga
    42241 gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa acggataaag
    42301 aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg gacaaagaag
    42361 tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc
    42421 ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac gagggagaga
    42481 cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa
    42541 tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa tgcgtttttt
    42601 acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga ttgaagacca
    42661 gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt
    42721 gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag aattatacaa
    42781 tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta tatcaggaaa
    42841 tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt cgaactattt
    42901 tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg tacaattttt
    42961 ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg acaatatccg
    43021 aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat ttgattcaga
    43081 aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat ctgattcgga
    43141 aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta caccgtttga
    43201 ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata atgctcgcta
    43261 tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag gcgtatggat
    43321 cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc tgcatgatat
    43381 tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc agtccgtcaa
    43441 tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga atatgatgaa
    43501 ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga
    43561 acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat tagcagaagc
    43621 attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat ggctcggaga
    43681 aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa aattacaact
    43741 taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga cagaagcacc
    43801 gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg atgggatcac
    43861 cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga tgcgttcttt
    43921 gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc agcagatgag
    43981 ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa
    44041 aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag aaataaaaca
    44101 aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa
    44161 acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt tggtttcatc
    44221 aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt tccaacagtt
    44281 caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc atttagagca
    44341 agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg
    44401 gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa aacaaatcac
    44461 ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag aaaattccta
    44521 ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa taaacaagca
    44581 aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga ttgggtatta
    44641 tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa aagttgaaaa
    44701 aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa
    44761 ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga
    44821 agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat ccttacgtag
    44881 taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat tgaatgccga
    44941 gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc aagaaaattt
    45001 actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa aatatctaga
    45061 tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat
    45121 aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc tattgaaaga
    45181 acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct
    45241 tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg aacaccccat
    45301 acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag aacaacaact
    45361 ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca
    45421 tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg caacgaatat
    45481 acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat ttcctgagat
    45541 aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag atagacgaaa
    45601 aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag cagaaaagct
    45661 cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc agctccagca
    45721 actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata gtaactgttt
    45781 agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc aactagctgt
    45841 tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga cacagcttga
    45901 tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat
    45961 agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc
    46021 acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc atgaagagca
    46081 attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg agacattccc
    46141 agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg aaataaagca
    46201 agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct acgatgaatg
    46261 tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat tacagcagct
    46321 ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt atgtgcttca
    46381 ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa
    46441 tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa ccggattaga
    46501 aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg
    46561 tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga tccagacaca
    46621 agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt cattagatga
    46681 agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag gaagaatgat
    46741 cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa taagaattga
    46801 tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag ggagattggt
    46861 aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga
    46921 aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg cagaagtctt
    46981 gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag attactgcca
    47041 atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg aactttattt
    47101 acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa
    47161 agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa
    47221 tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg agactaatca
    47281 gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat tggcatcacc
    47341 gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca cagaaaaaga
    47401 aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga
    47461 agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat tattaggtac
    47521 gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc cagtagaaca
    47581 agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc agataaatga
    47641 acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat tcaaagtgga
    47701 aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt tctttatgca
    47761 ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc aacaaacggt
    47821 ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag tgttcaagaa
    47881 aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga tcggcgacgt
    47941 agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat ttattttctt
    48001 tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat
    48061 ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg tgttacgtga
    48121 cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc agaaaaatga
    48181 aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt tccgcacaga
    48241 actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc gagagttgta
    48301 tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt catggctcga
    48361 tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt
    48421 gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt ttgattcaga
    48481 agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac gtacagaaga
    48541 aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat tggaaaaaag
    48601 caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc atccagaaag
    48661 ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc aaaagcaagg
    48721 ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag ccgatgaaat
    48781 gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag aagaacggaa
    48841 aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc aggcagaggt
    48901 gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa aagaacgtga
    48961 agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct taagacagga
    49021 tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg cacaaatggt
    49081 caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac ctcagcggtt
    49141 tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac
    49201 gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg cagaagaaat
    49261 tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt tgaaagactt
    49321 gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa aaactgttta
    49381 tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg aagcattcaa
    49441 gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt gctgcgatcc
    49501 tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag taaaagatct
    49561 actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat ttacagggat
    49621 gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt atttaagagg
    49681 cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga
    49741 tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg gagcagatac
    49801 ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc
    49861 acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg aaggaacgat
    49921 tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca
    49981 aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga ttttaagtgt
    50041 aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa gaaagccttt
    50101 ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga ttccatttta
    50161 tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac aaagattgtc
    50221 ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt
    50281 tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact ttttgcacct
    50341 agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag catatgaaat
    50401 tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt tgcttgagaa
    50461 atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag tttttttgtc
    50521 tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc cttcaagaaa
    50581 tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc ctcgtaaatt
    50641 ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc ttttagtgat
    50701 tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat tacttttggg
    50761 aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc tgcatctgaa
    50821 acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc atacatcgta
    50881 tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg gttttcattg
    50941 attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat
    51001 aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta tatttcagtt
    51061 tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta atcattatgg
    51121 tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag atttattttt
    51181 ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat ttatttgttt
    51241 atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata acagagaaaa
    51301 taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac tattaaagaa
    51361 ataaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga taaaataata
    51421 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
    51481 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
    51541 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
    51601 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
    51661 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
    51721 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
    51781 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
    51841 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
    51901 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
    51961 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
    52021 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
    52081 taatacgaaa cttggaaaag actattacaa agatagtttt tccaagtttt tttgttttta
    52141 caaaaagtat tcttgattag atgtataaaa aggtagtaga agatgatgat tgcacgaaaa
    52201 atagagaggg aaaattagct gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta
    52261 tagttaaata agatagcttg aaaggttgaa aaatccgctg gttttattaa attcgcttaa
    52321 cagcgagatt ttttttcttt acatttaagc tgatttcgct acaatgaatc tatattaaac
    52381 attgtggcaa agtgaggtgt agcaaaatgg aaatggaaca tattaatgaa aatacgatcc
    52441 gtgtactaat tggtcatgaa gatttagagg aacggggcgt ttcatttttg gatttacttg
    52501 gcaatcacaa agaaatcgaa aatttctttt atagtatttt ggaagaagtt gatatcgaag
    52561 aggagttccg aggaagcgaa gctgtcactt tccaagtttt gccaaaagga gacggattag
    52621 aactgtttat cagtaaaaat cttccggatg aagaaatttc tcaatcagaa gattctactg
    52681 ttgattcaga agatgtgagt gactttcttc gtcagcagat tattggcagc gatccggagg
    52741 aagaattatc tggaagcgcc ctagaagaac aggaaagaca agcaactttt gcttttgaag
    52801 attttgaaca agtgatccaa cttgcaacag atgtcgaact agagtctgct tggactgatt
    52861 tgtatttatt cgaagagcgc tattacctag tcgttcactt ctggatggaa aatctcaacc
    52921 aagcggatgt agagaatcag attgctcgaa ttttggagtt tgctgaaaag agcgatcgta
    52981 caccagaaac attgaatgaa tatggacaat gtctgatggc gcgtaatgct atcgagcgaa
    53041 cgaaatttta ttttgtataa atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg
    53101 cagtcttttt ttcgtataaa tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca
    53161 aacgaaaaat attgggaact atttttgttt agctgttctt attacttgat gcagaatagc
    53221 tttttcacgc cctctttttt cgtgtaaata aattcttaga cgtaaataag ttcagaggga
    53281 gaaaaatgct gcttgcaatg aatgaatata ataagtgctg ttttgctaaa catgcagaaa
    53341 aaggaaagaa atattattgt cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga
    53401 aaaaatgtgc tcattttgcc catcaaaaat cagataactg ttcggtcttt agtgaaagag
    53461 aatcaaaaga gcacttacag ttaaaggaat gcttcatgga ttggctggga cagtcagtag
    53521 aaccagtatt tttggaagcc tatttgccga ggttgcggca aaggccggat attttgttgg
    53581 cgaatctagc aatagaaatc caatgttcta gactgtctca ccagagattt attgaaagaa
    53641 cccaaaatta tttgaataac agttaccaag tatggtggat acttggtaat tcttttcttg
    53701 gacaatcgca attctcattg atagaaaaaa gttgctgcta ctataataga aaaagaggag
    53761 tgcattgctg gaaagctgat ctcaagaaac aaaagcttta tttgtatcat catattacag
    53821 agactgtatc agggcatatt tccttttgca gtagctgctg gactttttct agtagggacc
    53881 ttaaagaaat attcactagt aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag
    53941 tgagtgaaga tgggaagaaa tggctgacac gccaacttat tcacaagcaa aagaatacag
    54001 tatccataca agaacaatgc tacttgcgtc ataaacatct tttgcatcta tcatcgtgga
    54061 tctatcaaaa aagtcggttt ttcttttatt tacaagaaca ggtttttctt tatcgaatgt
    54121 tgtacgaaga agccttggaa aaccagaaag ttcctgattt caactcatgg ctttgccaaa
    54181 taaaagaaca taaaagggaa tggctttttc ctatgattga tgaagagatg gtctatcagc
    54241 agttttttaa cgaatgcata cacttgtctt ctttgaaaaa atagtaagta taaaaatagg
    54301 ctaggaatcg gttttctatg taaaacaaga tttagttatg ctaaaattga tggaagcaga
    54361 cacatgaatg agtagaaaag ttagatgctg tgacatgtgg atgttagact atgatctatt
    54421 ttgatagata gagctaaggg aagaaaagga gtggatttca tggaagtaaa gcagttgcca
    54481 aaacgagaag aattgcctga aaatttaact tgggacttga ccaagatctt ttcaagcgac
    54541 caagagtttg atgagaaata tttggaatta tcagaagagt taaaacaatc tgaaaaacac
    54601 aaaggaacac ttgatcaagg cgcttctcaa tttttaaatg cgattgaatt cgtattgagg
    54661 gtttatcgcc aaactgaagt catttatgta tatgcgcacc ttaaaaacga tcaagacact
    54721 ggaaatacag attaccaagc gctttatgca agagcaagca gtctgttttc gaaagttagt
    54781 gaagccgttt cctggtttga accagaaata ttgcaattgt cagatgacca gatttggcaa
    54841 tatttcaaag aagaaccaaa attggaagtc tatcgccatt atatccagca aatagtagat
    54901 aatcgagccc atgtcttatc tgctgagcag gaatctcttc ttgctggagc aggtgaaatc
    54961 tttgatgctt caagtgatac atttgctgtt ttgaataatg cagatctagt ttttccaacg
    55021 attgaaggag aaaatggtga aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta
    55081 gaaagcacgg atcgaagggt gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa
    55141 caatttagaa atacatttgc ttctacttta ggcacacata taaaaggaca taattttaaa
    55201 gcgaaagtcc gtaattacag ctctgccaga gaagcgtctt tgagcaataa tcatattcct
    55261 gaaagtgtat acgatacttt ggtagacgtg gtaaacaagc atttgccttt gttacatcga
    55321 tacatggaat tacggaaacg tttattagaa gtggaaaaac tgcacatgta tgatctttat
    55381 acaccggtct taggggaagc tccaattacc tttacgtacg aagaagcaaa agaaaaagct
    55441 ttagaagcac tgaaaccaat gggtgaagaa tacatggcca tcgtagaaaa agcattctct
    55501 gaacgttgga tcgatgttgt cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga
    55561 agctatgaca caaatccata tattttattg aattggcatg atacgctgga tcagctattt
    55621 acgcttgtcc acgaaatggg acatagtgtt catagttatt tcactcgttc gaaccagcct
    55681 tatgtgtacg gcgactactc catcttttta gcagaaattg cttcgacaac gaatgaaaat
    55741 atcctaacgg agtatttatt ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc
    55801 aaccattact tggatgggtt taaaggaaca gttttccgcc agacacaatt cgctgaattc
    55861 gagcatttca tgcatacgga agatgaaaaa ggtgtgccat tgactagtga atacctaagt
    55921 gatagttatg gtaaattgaa tgcaaaatat tatggtccag cagtcgaaga agacccagaa
    55981 attaaattcg aatggtcacg gataccgcat ttttattata attactatgt tttccaatac
    56041 tcgactggct tttctgctgc ttcagcactg gcgaagaaaa tactaaacca ggaaccagag
    56101 gcactagaga actacttggc ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta
    56161 atgaaaaaag caggagttga tatgacacag gctgcatata ttgaagatgc aatgtcgatg
    56221 tttgaacaac gtctaaatga attagaagaa ttgatcgatc gtttataagg ctttctattt
    56281 ttgattttct cgaatagaaa cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt
    56341 atttttt
//
LOCUS       DAFFFQ010000010        54446 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000010
VERSION     DAFFFQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.010"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            10..588
                     /locus_tag="LQB69_000690"
     CDS             10..588
                     /locus_tag="LQB69_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524877.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(828..911)
                     /locus_tag="LQB69_000691"
     tRNA            complement(828..911)
                     /locus_tag="LQB69_000691"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(875..877),aa:Leu,seq:gag)
     gene            966..1145
                     /locus_tag="LQB69_000692"
     CDS             966..1145
                     /locus_tag="LQB69_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524878.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1294..2511
                     /locus_tag="LQB69_000693"
     CDS             1294..2511
                     /locus_tag="LQB69_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3524879.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2695..3531
                     /locus_tag="LQB69_000694"
     CDS             2695..3531
                     /locus_tag="LQB69_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3524880.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     NEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3607..4755
                     /gene="nagA"
                     /locus_tag="LQB69_000695"
     CDS             3607..4755
                     /gene="nagA"
                     /locus_tag="LQB69_000695"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL3524881.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4976..6034
                     /locus_tag="LQB69_000696"
     CDS             4976..6034
                     /locus_tag="LQB69_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL3524882.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6038..7072
                     /locus_tag="LQB69_000697"
     CDS             6038..7072
                     /locus_tag="LQB69_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL3524883.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7174..7890)
                     /locus_tag="LQB69_000698"
     CDS             complement(7174..7890)
                     /locus_tag="LQB69_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3524884.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7859..8644)
                     /locus_tag="LQB69_000699"
     CDS             complement(7859..8644)
                     /locus_tag="LQB69_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL3524885.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8635..9384)
                     /locus_tag="LQB69_000700"
     CDS             complement(8635..9384)
                     /locus_tag="LQB69_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL3524886.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9682..10206
                     /locus_tag="LQB69_000701"
     CDS             9682..10206
                     /locus_tag="LQB69_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL3524887.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10222..11721
                     /locus_tag="LQB69_000702"
     CDS             10222..11721
                     /locus_tag="LQB69_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL3524888.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11809..12411)
                     /locus_tag="LQB69_000703"
     CDS             complement(11809..12411)
                     /locus_tag="LQB69_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3524889.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12515..13471
                     /locus_tag="LQB69_000704"
     CDS             12515..13471
                     /locus_tag="LQB69_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL3524890.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13776..14732)
                     /locus_tag="LQB69_000705"
     CDS             complement(13776..14732)
                     /locus_tag="LQB69_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3524891.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14906..15466
                     /locus_tag="LQB69_000706"
     CDS             14906..15466
                     /locus_tag="LQB69_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524892.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15459..16283
                     /locus_tag="LQB69_000707"
     CDS             15459..16283
                     /locus_tag="LQB69_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL3524893.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16293..16910
                     /locus_tag="LQB69_000708"
     CDS             16293..16910
                     /locus_tag="LQB69_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL3524894.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16928..17611
                     /locus_tag="LQB69_000709"
     CDS             16928..17611
                     /locus_tag="LQB69_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL3524895.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18294..19577
                     /locus_tag="LQB69_000710"
     CDS             18294..19577
                     /locus_tag="LQB69_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3524896.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19593..20480
                     /locus_tag="LQB69_000711"
     CDS             19593..20480
                     /locus_tag="LQB69_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524897.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20497..21333
                     /locus_tag="LQB69_000712"
     CDS             20497..21333
                     /locus_tag="LQB69_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3524898.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21336..21959
                     /locus_tag="LQB69_000713"
     CDS             21336..21959
                     /locus_tag="LQB69_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524899.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21956..23248
                     /locus_tag="LQB69_000714"
     CDS             21956..23248
                     /locus_tag="LQB69_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3524900.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23607..23704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23820..24401
                     /locus_tag="LQB69_000715"
     CDS             23820..24401
                     /locus_tag="LQB69_000715"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL3524901.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24404..25711
                     /locus_tag="LQB69_000716"
     CDS             24404..25711
                     /locus_tag="LQB69_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL3524902.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26127..26615
                     /gene="purE"
                     /locus_tag="LQB69_000717"
     CDS             26127..26615
                     /gene="purE"
                     /locus_tag="LQB69_000717"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL3524903.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26608..27732
                     /gene="purK"
                     /locus_tag="LQB69_000718"
     CDS             26608..27732
                     /gene="purK"
                     /locus_tag="LQB69_000718"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL3524904.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27745..29040
                     /gene="purB"
                     /locus_tag="LQB69_000719"
     CDS             27745..29040
                     /gene="purB"
                     /locus_tag="LQB69_000719"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL3524905.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29220..29597)
                     /locus_tag="LQB69_000720"
     CDS             complement(29220..29597)
                     /locus_tag="LQB69_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524906.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29853..30581
                     /locus_tag="LQB69_000721"
     CDS             29853..30581
                     /locus_tag="LQB69_000721"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL3524907.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30581..30835
                     /gene="purS"
                     /locus_tag="LQB69_000722"
     CDS             30581..30835
                     /gene="purS"
                     /locus_tag="LQB69_000722"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL3524908.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30837..31508
                     /gene="purQ"
                     /locus_tag="LQB69_000723"
     CDS             30837..31508
                     /gene="purQ"
                     /locus_tag="LQB69_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL3524909.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31509..33731
                     /gene="purL"
                     /locus_tag="LQB69_000724"
     CDS             31509..33731
                     /gene="purL"
                     /locus_tag="LQB69_000724"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL3524910.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33716..35155
                     /locus_tag="LQB69_000725"
     CDS             33716..35155
                     /locus_tag="LQB69_000725"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL3524911.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35178..36230
                     /locus_tag="LQB69_000726"
     CDS             35178..36230
                     /locus_tag="LQB69_000726"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL3524912.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36227..36805
                     /gene="purN"
                     /locus_tag="LQB69_000727"
     CDS             36227..36805
                     /gene="purN"
                     /locus_tag="LQB69_000727"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL3524913.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36802..38337
                     /gene="purH"
                     /locus_tag="LQB69_000728"
     CDS             36802..38337
                     /gene="purH"
                     /locus_tag="LQB69_000728"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL3524914.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38353..39627
                     /gene="purD"
                     /locus_tag="LQB69_000729"
     CDS             38353..39627
                     /gene="purD"
                     /locus_tag="LQB69_000729"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL3524915.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39749..40663
                     /locus_tag="LQB69_000730"
     CDS             39749..40663
                     /locus_tag="LQB69_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3524916.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40796..42361)
                     /locus_tag="LQB69_000731"
     CDS             complement(40796..42361)
                     /locus_tag="LQB69_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL3524917.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42354..43805)
                     /locus_tag="LQB69_000732"
     CDS             complement(42354..43805)
                     /locus_tag="LQB69_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3524918.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43819..44835)
                     /locus_tag="LQB69_000733"
     CDS             complement(43819..44835)
                     /locus_tag="LQB69_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3524919.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45055..46143
                     /locus_tag="LQB69_000734"
     CDS             45055..46143
                     /locus_tag="LQB69_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL3524920.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46197..47723
                     /locus_tag="LQB69_000735"
     CDS             46197..47723
                     /locus_tag="LQB69_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL3524921.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47725..48930
                     /locus_tag="LQB69_000736"
     CDS             47725..48930
                     /locus_tag="LQB69_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3524922.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49187..50164
                     /gene="guaC"
                     /locus_tag="LQB69_000737"
     CDS             49187..50164
                     /gene="guaC"
                     /locus_tag="LQB69_000737"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL3524923.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50428..51852
                     /locus_tag="LQB69_000738"
     CDS             50428..51852
                     /locus_tag="LQB69_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL3524924.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52313..53422
                     /gene="pdhA"
                     /locus_tag="LQB69_000739"
     CDS             52313..53422
                     /gene="pdhA"
                     /locus_tag="LQB69_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL3524925.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53425..54402
                     /locus_tag="LQB69_000740"
     CDS             53425..54402
                     /locus_tag="LQB69_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL3524926.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17402 a         9317 c        11921 g        15806 t
ORIGIN      
        1 tttttagaca tgaatatctt atttttattg ctatttttct taattttcct tctaacatat
       61 aggaaatgtc aaaactatta tgtgcgtata ttgactgcat taccgttttt cttaaacctg
      121 attatttatt ttggtaatac aatgggtcaa agctttactt acgtcaatgg aaataaaagg
      181 gcaatgattt ggagtagtag taacctgaat aatctcttta cagagttagg aacgaaactg
      241 tcactttttt atccaggtac ttggatagct actttagtag tcttggcatt attactatgt
      301 ttaattgttg gaatttattt aagttttgac aataagaaaa cttctatttt tttagttatt
      361 ttgatgataa tgggattttg ttcaaggtta attatgggat tttctcccac agtatgggct
      421 tccggaatgc ggacgtacta tatactttat gttgttatcg ccatcttagt actaatggca
      481 gttaaagagc taatgaaaag catgagtgta caaaaaaatg aatttatgca atttggatta
      541 accgtgcttg gtatttgtac ctttataata acggtgataa acagataata tacaaaaaat
      601 ccctcgcttt agtattaggt gagggatttt actcgttttt cgattaattg ttttaacact
      661 tgcaagtttc tacgagtagc ttgtttccga ccttcatata ctcacctcag gctgtttaca
      721 tgattgtacg ttctttttgt aaaggataca gtgatatgta tataatggca taaagaaacg
      781 ttcaaacaaa aaaactcgtg aagcaaaatt aacttcacga gttgtagtgc agatgatggg
      841 actcgaaccc acacgagcta acgctcacat gaccctcaat catgcttgtc tgccaattcc
      901 aacacatctg cgtaataata acacactaat gatagcgagg ttttacatag ttgtcaagct
      961 caaaaatggg aggacttatt aagatggaga aatatgacgg agaattttca ggactgggaa
     1021 tgatcttagg tgtattgatt ggacttgcat tcggacgttt tttgcttggt tttatgctag
     1081 gaatcatttg tggaatcgca atggattggg cagcgaatct atggaatgac tatcatgaca
     1141 attaagttct caacgcttgt ttgtgttttt ttaatactta cgtttttcct aaataagtgg
     1201 caaaattttc taggatggag tatgatattg aaatagtaaa taacaaaaaa tttggaaagt
     1261 tctaactttt tgaaatgagg gattggctaa gtgatgagag aatttgaaaa atccaacaaa
     1321 ttagaaggtg tcagttacga tgttcgcggt cccgttctag aagaagcgga gcggatgcaa
     1381 gaagagggca ttagtatttt aaaattgaac acgggaaatc ccgcaccttt tggatttgaa
     1441 gcaccaaacg aagtcattcg tgatttgatc atgaatgccc gtgactcaga aggctactca
     1501 gattccaagg ggatcttttc tgctagaaaa gcaattgagc aatattgcca actgaaacat
     1561 tttccgaatg tgacgatcaa tgatatctac accggcaacg gagtgagcga attgatcacg
     1621 atgtgtatgc aggggttgct ggacaatgga gatgaagtac tggttccaat gccggactat
     1681 ccgttgtgga cggcttctat tgcattagct ggaggcacac ctgttcatta tatttgcgat
     1741 gaagaggcag aatggtatcc tgatattgat gatatcaaat caaaaataac gagtcggaca
     1801 aaagctattg ttatcattaa tcctaataat cctactgggg cactttatcc aaaagaatta
     1861 ttagaagaaa tcgttgaagt agctcgacaa aataacctga ttatttattc cgacgaaatc
     1921 tatgatcgct tagtgatgga tggtctagaa catattccga ttgctacatt agcgcctgat
     1981 ttgttcgttg ttacgctgaa cggtctatcc aaatctcatc gtgtagcagg atttcgagta
     2041 ggctggatgg tcctaagtgg agataagtca cacgtcaaag gctatataga agggctgaat
     2101 atgttgtcct ccatgcgttt gtgttccaat gtattgtctc agcaaattat ccaaaccgcg
     2161 ctaggcggtt atcaaagtgt cgatgagctg ctcctgccag gcggaaggat ctatgaacaa
     2221 agagaatata tctataatgc aatcaatgat attcctggtc tttcagccgt aaaaccaaaa
     2281 gctgcttttt atatattccc aaaaattgat acaaagcgat tcaatatttt ggatgatgaa
     2341 aaattcgttt tggacttttt acatgaacat catattctac ttgtacatgg tggtggattt
     2401 aattggaacc agccagatca ttttcgaatc gtttatcttc ctaaaatgga tgatttgaaa
     2461 caaacagcta agaaaatgag agaattccta gcaacctatc gccaaaaatg attttaaaaa
     2521 ccaaattttt cagtaggagg gccacagaca aacacactga ggctctcttt ctagtggagt
     2581 tttcttaaat aactaaagta tattatttag ttgcaagtct tttcttgaag tttgcgactt
     2641 cctaaagtaa aatacgaata gaatgcgaat acgcatattt tggggaggat acttatggaa
     2701 acgttaatcg agtcctttgg aacgatcatt aaagagattc gaaaagaaca aaaaatgacg
     2761 caaaaaatgc tttcgcatga tatttgttca caaagtgtat taagtcggat cgaaaataat
     2821 gaggaactgc cgaatgtatt ggtgatgatg cagatttgtc aacggctagg ggtaacgatt
     2881 gatcatgtga tgaatctctc tcgaaaaact actcggacaa acgataaatt atttgaactg
     2941 ttggatagtt gtttccaaaa acgtgattat cggaagttag aacaagtact aaaatcttct
     3001 gatatttctg aaaatctgta tcaagcaact gactttcaac gctattatta ttatctcggt
     3061 gtttgcgaat tcacgttgcg tcaaaatatc tctcagtccc tttattattt gaaagaagca
     3121 ttaagctact ctagccaaaa agaaagaatc catgtttctg acacagaaat acaactgatt
     3181 agttgtatcg gaaaaatata tggtgtggca ggaaaaacgg ctgaagcacg ctactatttg
     3241 agtcgaagca tccaactgct gcatcaatca gctgatgaac gatcaaaaag tgaattttcg
     3301 caaatttttt ataattatgg aaatttctta ttccatcaaa atgaaatcaa cgaagcgtta
     3361 gaacaggtga atcaaggaat ctattgggct caagagaaga atagctacta ttacctcaat
     3421 gatttatttg tgcttaaaag cttaatatac aagcgtaaag aagaaccaga aagagcagca
     3481 ttctatgaag gcttagcaca agctgtgaaa caaatttcaa aaaatttgta aatggtgtag
     3541 accattcttg acttcttctc taaataagag tactttagag gtagaaaaac aaagggagag
     3601 aatagaatga gaacttttat ctatgctgac aaattctttt tagcgtcagg tgtcaaacat
     3661 gcaggctatc ttgaaattac cgatggactt tttggtgaat acaccaaaga actacctgag
     3721 ggagaagtca acatcattga ccaaagcggc aaatggattg ctcctggttt ggtagataca
     3781 catattcacg gctatatgaa tcatgatgta atggataatg atgcagaagg catcaaagtg
     3841 atgtctgaag gtctgttatc ttgtggagtg acttcatttt taccaacaac attgacgtca
     3901 agcaaagaac gtttaaaaga tgtagctgag acaatcggca aaatgtatca ggaagcacca
     3961 ggagctaaaa tcaaaggaat ctattttgaa ggtcctttct tcacagaaga acacaaaggg
     4021 gcacaaaacc caagttattt cggggatcct gatttggata ctttccatga atggcaagaa
     4081 gcttcaggtg gtttaatcaa aaaaattgct ttagcaccgg aacgtcaagg tgtagaagag
     4141 tttgtcaaac aagtgactga agaaggagta gttgtttctc taggacacag taatgctaca
     4201 ctagaagaag cgcaaaaagc tgttgaagca ggcgcaagtg tatttgtcca tgctttcaat
     4261 ggtatgcgtg gattgaatca tcgcgaacca ggtatggtgg gtgcgttatt atctttagaa
     4321 catgtctttt ctgaattgat ttgtgatggc catcatgtac atccaaatgc tgcgggaatc
     4381 ctaatggaaa aagcaggaca tgatcatgtc gcattaatta cagactgtat gatggccggt
     4441 gggatgcctg atggaaatta caatttaggc gaatttccag tagtagtaaa agaaggaaca
     4501 gcacgtctag aatctggcaa cttagctgga agtatattga aattgaaaga agcaattaaa
     4561 aatgttgtag actggggatt agcaacacca gaacaagcag tcatgatggc tagtctgatt
     4621 ccagcaatca gctgtaaaat cgatgataca tgtggaaaaa tcgctcaagg acgcgatgca
     4681 gactttatcg tattgaaccc tgatatgaca ttggaagcaa cttatctaga tggcgtggaa
     4741 cgtttccacg cataatataa tttaagttaa ccttaaaact aaacttctta agtttggttt
     4801 taaggttttt tttgtgttct agtaaaaatc actttacata agtttcaaaa gtttctttaa
     4861 tttcctattg ttcaataagt aacaatgtaa agataaaatt aaaaatgtta atacgtatta
     4921 gcaaaataaa agcttttttt attgtgaata aatgaacata attaggagga gaaagatgaa
     4981 cttatttgac atttttcgat cgactttgac agatatgtcg attgtcagtg caatcacttc
     5041 aacagttttt attatattat tagggtttgt ttgtagaaaa aaggggattt tttctgaatc
     5101 tgttggaaaa attctatcga aagtcgtctt aacagtggct ttgccagcac tggcatttcg
     5161 agctttcatg caagatatca atacagaatt actgatgcaa ggaatcaatg tcttgatttg
     5221 gggattagtt atttatatta ttcttattct agtttcaaaa ccttttttct taagctataa
     5281 aggggataag caaacaacgt tgcgtgtcct gacgatcttt ggttctacga cctttttcgg
     5341 aacgcctatc gtcagtgcga tttatggtcc tgtcggcgtg atgtattcct caattttcaa
     5401 tattggctac cgaatctttt tgtattctta tggatacata aaaatgagtg gcttgaagat
     5461 ggaagctaaa aatatcaaag cgatgttcgt caatccaatc attatcgcta cttttgccgg
     5521 ccttttcatt tggctgttcc aagggtatct gccacaagtg tcagttacag gtgcagaagg
     5581 agagataagc caagtagcgt ttttgcgaat tgaccagact gcaccgtggc tatttcagcc
     5641 gatggattat ttagctggtt taagttcacc attggcgtgg ctagcaatcg gcgcaacatt
     5701 aggtgaagta agcttcaaaa aagctgcatc tgaaaaaact tcatggtatt acagtctagt
     5761 caaagtggta tttgtacctg cattgaatat cgtgattcta gcagttttga cggcaacgaa
     5821 tattctacca gtcaattttg aagcactggc aacaatcgtt atcatgatgg ctacaccagc
     5881 tgcaacagtg gcagcagcct atgctatcag ttttgatagg gaggcacttt tagcctccaa
     5941 tgcatcctta ctgtcaacta tttttgctgt ggtactgaca cctgtatgga tcgttgtatt
     6001 acaaatgatt catggcatag gtttattttc ataagaaatg gaggaagaaa gtatgagaat
     6061 cgcatgttac ggtgttcgtc cgaacgagat agattttttt gaaaaattga atatttacca
     6121 ttatgatctc agtctttatg aagaactgtt gacacatgac aatatcgtgt cagcaaagga
     6181 ccatgatgct gtattgttga ggggaaattg tatagcagat gagaccaatc ttgcaaaaat
     6241 gcaggaatat gggattcgtt atgtttttac acggactgtg ggagtcaatc atatcgatct
     6301 acaagcggca gctgattttg gtatgacagt agcccgtgta ccatcgtatt cgccaaatgc
     6361 gatagcagag ttatctttga cttttgccat gatgcttttg agaaatactg cgtatacgac
     6421 gatccgaaca tctttcaaag attttcgagt ggatgagcaa atgtttagtc gtgaaatcag
     6481 aaattgcaca gtagggatca taggaacagg caggataggg ttgacggaag ctgttttgtt
     6541 taagggacta ggagccaaag tgatcggcta cgatattcat cagacagaag ctgctaaaaa
     6601 agtattgacg ttccactcgc ttgaagagtt attagccaaa agcgatattg tcagtattca
     6661 tgtacctcat atcccaggtg aaaacgatca aatgatcaat gaaggatttt tagctcaaat
     6721 gaaaaaaggt agcatcttga ttaatacagc ccgtggagag cttcaagata atgaagctat
     6781 tttaagagct ttgaaaagca atcatttagc tggttttgca accgatgttt ttgcaaatga
     6841 aacagaggtt tttttccgat ccttcaagcc atgggagacg attccagacc ctgctatcca
     6901 gcaattagta gaaatgtacc ctagagttct tgtaaccccg catgtaggtt caaatacaga
     6961 tgaggcatta tcaaatatga tttcgacaag tttcgagaac tttcgtgaaa taatagaaac
     7021 tggaaaaacg aaaaatgagg tatctcttcc taaagcccgt caattaagat aagaaaacat
     7081 ggatattcgt caataaaatg tttaaaaata gtccaagtaa acagttgtta cttctgtata
     7141 cttggactat tttgtatggg ataattcgat gactcaacag taatctgtga gctttttccg
     7201 cccattcttt gtcaattcat aacaggttag cggccggccg atttccaaat atttgattct
     7261 ttcgttcaat atatcttgtt ctactaaaaa attcaaatat ttcttgacgg atatacgtga
     7321 cagtgagctt ttttgagcca gttcttttgt tgaaaaaata gtatcttctt ttagaataaa
     7381 tgcacttatt ttttttaaag tcagttttga cagaccttta ggcaaatcga tgaatgatgg
     7441 tatttcgtga atgggagtgg tgtggttgaa tacttgatcg atcaatttct gatcggcttt
     7501 ttcagtcgtt tggattgtct gagagaaagc aagatacttt tcgatcgcca gctgaaaacg
     7561 ttgcaaagtg aacggtttga tcaaataatc taaggcaccg tagcggatca cattctttat
     7621 ggtttcgata tcatctgcag cagtgatcaa aatcactggg atatcatttc cttgctgtct
     7681 aagccaagat aaaaaatgga taccactttc gccaggcatg tatacatcaa gcagcagcag
     7741 atctgcgttt ttttgtctga aatatttttt tccttcttct acatttctga aactttcgat
     7801 tacgttcata ttagggatgc tttccacaaa agtacgattc aattctgcaa ccattggatc
     7861 atcttcgatt atcaggacat ttgtcatcat tttgatcttc cttttttggt aattctagat
     7921 agacgattgt accgccattt gtttgtttgg aaaattctaa aaatccaccg tggcgtttga
     7981 cgattgaatg gatgagattc aatccaaacc cacgattgat tcccttcgtc gaaaaaccta
     8041 gcgtaaatat tttttctgag atttcttctg atatcccgac accgtcatcg cagacggtca
     8101 cgatgaatac ttcagcttct gtgtcatagt ttaaatgaat ccggacctgt ccgtttcttt
     8161 catgtttttg tagcacggct tcttttgcat tatcaagtaa gttccctaac acttgcaaaa
     8221 gtgaatggat ggagtcatcc atctccattt tagggataaa agaatcgctt tctaacagca
     8281 tttcaacaga ttgttctttg gcttcgttta ttttgcctaa taaaaaaccg gcaattgcag
     8341 gtactttgat cagacttgag acttggccaa tctctttttt ataattttga tttagctgct
     8401 ggataaaatg ggtgacttct tcatacttct tcaaatcaat caaaccagaa atgacatgaa
     8461 gtttattcat gaattcatgg gtttgagctc tcatggagtt tatatattgt ttcgtccccc
     8521 caagctcctc cattaatttc attacttctg atcgatttcg taaagttgcg actgctccta
     8581 aaaaacggtc attgctaaaa agaggtgtaa cgctagaaat agtttctatc ccatttaaat
     8641 acatggattg agtccgaacg gtcgtttgat gctcaatcgt ctctttaaac aaaacgtcat
     8701 atagattatc actgattttt ttcctacttc gaaatccaat agctgctctg tttggtagaa
     8761 aatgtttttt gcaggtgtat tgatcaagag gatagtttta tcagaagaga tggctaagat
     8821 tccttcatct acttcatttt cgataatcgt tttttcactt aactgttctg ctatttcttg
     8881 tggttcaagt ccaagcagta cttttttcat tttttgggca agaaaaacgg cgcctatgat
     8941 gcctacgcta agtccaataa tcaatgaaaa aacaatcatt ttttgtccat ctttgacttg
     9001 attgtcaatc gtgttgtttg ttaagccaac acaaactgca ccaataatct cttgatcata
     9061 ataaataggg acgaaatagc ggaagccgaa ccctaacatt ccttcttttt cagagaaaaa
     9121 ttcttgtcct ccaatcactt ttttcacatc ttcatgactt gagaaaggaa gaccgatgta
     9181 acttgggtct gcgtgagaat atcgaatgcc ttcagggtcc atgacaacga taaaatctaa
     9241 attcgatgca atcatgattt catttgttat tccttgaatc gcatgtgtcg ggtcaccagt
     9301 ctgcagcttt ttttggatga gtttatcatt ggcaacgatt ttagcaatgc tagagatttt
     9361 atctgacaca ctagatcggt tcatcgaaag aataaaattc ctgatcaata tggcagaaac
     9421 aatcaaagaa acgaatgtgg agaaacaaac gacaaggatg atcgtgttct ggagctttaa
     9481 tggttttttc ttaaaaaaag gaaacatagg ctacctcgaa agaatttatt gtgaaaattt
     9541 taacatgaaa acaaaaattt aacaaattgt caacgataaa cacaaccttc ctataaaaaa
     9601 caccactgag tcggtaaaag aagctataca gaattttcaa acattggtat gatagacgta
     9661 gaaatggaag gaggcgtgta tatgataggg ggaatcgcac ttgagacgat ttattggtat
     9721 acattgctga tttgtgcagg attggcagtc ctattgatta tttttggaga tgtattcgat
     9781 tttgatggcc cgcttgatcc gatgcttatt atcccttggt taactttcac aagcttattt
     9841 ggttttctag gtgagcgttt aacagatcaa tcatctttat ttttatttat tgctagtgga
     9901 ggcatatcga tagtgttggt atttttgctt aatttctata tattgatgcc gatgaaacat
     9961 gccgaatcaa cgatgtcgtc ttctgaaaaa actctggaag gacaagtagc tgttgtgatc
    10021 acaccgatcc ctgtacgagg tatgggagaa attcagttga aaagtgttac cggttccatt
    10081 agtcgtccag cttgttttta cagcccccaa gaacagacag ctgagcgggg aacgaaagta
    10141 ttgatcattg agatcaagga aagaatctgt tatgtcatgc cttatgaaag catgataaaa
    10201 gcttaaagga gcgtgtgtta aatggaaata ccagttgttt ttatcgcgat cgcaatcgca
    10261 gctttgattt tactgatgtt gctgatcgtc tttgtagcga agtaccagac agcaaaaccg
    10321 gatgaagcgt tgatcatcag tggaagttat ttaggaagca aaaatgtgca cattgatgaa
    10381 agcaataaca aaatcaaaat tgtgcgtgga ggtggcgcat tcgtcttgcc agtttttcaa
    10441 cgttcgaatc ggatcagtct attatcgagt aaattggacg tctccacccc tgaagtgtat
    10501 acggaacaag gcgtaccggt gatgtgtgat ggtacttcga tcatcaaaat cggttcatct
    10561 gtagaagaga ttgctacagc agcagaacag tttttaggca agacaagaga agaactggaa
    10621 aatgaagcaa gagaagtatt agaaggacac ttgcgctcga ttcttggttc tatgactgta
    10681 gaagaaatct atcaaaatcg cgataagttc agtcaaagcg tacaagaagt cgcttctgta
    10741 gaccttgcaa aaatggggtt gatcatcgtt tctttcacta tcaaagaggt cagagacaaa
    10801 aacggttatc ttgactcttt agggaaacca agaatcgccc aagtcaaaag agatgcagat
    10861 atagcagaag cggaagctct aaaagaaacg cgtatcaaaa aagctcaagc agaaaaagaa
    10921 tcacaagcag ctgaattaca gcgacaaaca gagatcgcag aatcattaaa agaaaaagaa
    10981 ctgaaattag caacctacaa acaagaacaa gatgttgcta aagcaaaagc agaccaagcc
    11041 tacaatttag aaagtgcccg agcacaacaa caagtaatcg aacaggaaat gcagattaaa
    11101 atcgtagaac gtcaaaaaca aatcgaactg gaagaaaaag aaatcacacg tcgtgaaaag
    11161 caatatgatt cggaagtgaa gaaaaaagca gatgccgatc ggtacgctaa agaacaagaa
    11221 gcacaagctc aaaaagtcaa agaagtgact gaagccgaag cagaacgatt ccgtgtggaa
    11281 gcactggcag aagcggaagc caataagaca cgtttagctg gacaagcaga agccgaagca
    11341 gcactagcaa aaggaaaagc agaagccgaa gcaaaacaaa agatcgccaa tgccttcaag
    11401 gaatatggcg aagcagctgt cttgagtatg gttatcgata tgctaccgca attgatgcgc
    11461 gaagcagcac agccgctagg aaatatcgaa aaaatctctg ttgtcgatac aggaagcagt
    11521 tcaggagaaa ccagtggagc gaaccgagtg acaaattatg ccacaaattt gctttcttct
    11581 actcaagaaa cactaaaagc gacaacagga cttgacttga aggaaatcgt agagaatttg
    11641 tcgacacgtg ggactactag ccaagtaaat atgtatgaag cgccaaaaga ggcagaacaa
    11701 aactcgatga aggaagaata aagtagtaca cttcaccgtt caatcaaaaa tggaggaaga
    11761 ctttttaaag ttcttctctc cattttttta ttttattgca aaagaaaatt agtcctcgct
    11821 atttttcttt tgtttttgga tcttagcttc tatctcttgt aaatttgttc ttagcaatcg
    11881 ttctttcttt tggagttcga ccaaatcttc tgatagttct tccttctttt ctgtttcagg
    11941 aggacgaaga aagaaattga taccgattgc aatgaatgtc cctataaaag tatctagtac
    12001 acgttggata gcatacaaag cggattcgcc ttgagggatg ctcaacgcaa tcagcaacaa
    12061 cgtagctatt gcggaaatga taccggaatt attgttaata ccatctgaca cgacgatcac
    12121 aataataact agaataggaa gcaagatcaa ttctaccaaa aaatcattgt gaaaatagct
    12181 tttgacaaaa aaataaatga tagctaatgt accacctaat gtattgccga tgatcctaga
    12241 ctttccaaaa gaaacggttg tagtcaagtc ctgacgcaaa gaaaaaacag cagataatgc
    12301 agcaatcatc gggattcctc gttgagtcaa atgaaaaaac aagatacata aaaaaacagc
    12361 taaggcactt ttgatcgtac gcatacctag tcgaaaacgg ccaaattgca tagagattct
    12421 cctttttggt ctttttacta tcatacctaa acttttgtct gatagctagt ttttcattat
    12481 tcaagtataa ttaaaataaa aaagggggaa gtaaatgcgt ataggaatgc gaacagtcaa
    12541 aacagtgatc agtgtcttct tttcgatggt cacagcgggt agcttatcgc tgttgtattg
    12601 gccagctgca gggatcattg cattattaag tgtaggaaat accaagcgct ctacattgcg
    12661 tacgggcatg aatcgaatcg cagcctttat attagctacg gcaacagcac tgttcagttt
    12721 ttttgctgtt gggtatacta tttttggctt cagtcttttt ttattcctgt tcatcccgct
    12781 tgcctctcgc ttcaaattaa ccgaagggat agtagtaaat tcggttttgg tcactcatta
    12841 tttagtggag gaaaacatgt cttggcagct gatcggcaat gaagcgggat taatggcgat
    12901 tggattagta tttgctttgc ttgcaaatgt ttacatgcca gatcgtacga agcagctgaa
    12961 ggaaaaccaa atacagatcg aaaaagaatt tcgaaatatt ttgagacaaa tggcagaatt
    13021 tcttttatca gaaaataaag aagatgtgca gatcaaatgc gaacatttac tgacattcat
    13081 tcgcgaaagc caagaagatg ccagagaaca tcaagaaaac tactggctca gacagccgct
    13141 atactacgaa acttacttct ctatgagaag agcacaggcc aatgtgatta aagacatgct
    13201 ggaaaatcta gagagaatcc agcaaccagc ttattatgga aaacatatct atggtttatt
    13261 gatttatact gcagaaacat tttcagagtc aaatgatggc agacaaattt taacacgtat
    13321 tgaagaagtt tatgtattgt atcggcaaat gcctctgccg actagtcgcc ctgaattcga
    13381 ggatcgagca gaattgttcc agtttttgca gtcttttaaa agttttattg aaatcaaggc
    13441 agaattctcc caacaaaaaa tccaaaaata gaggttgtga attcagctaa tattcaggtc
    13501 aaaaagctga tttcacaacc tcttctttaa acggtgttct cctgtcaact cctcggtcac
    13561 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgctcct tgtgactcgg
    13621 agctgaacgg taggctcaca acctctttct taaacggtgt tcaaaagctt tcctctgcgg
    13681 taataagtcc agccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac
    13741 ttgatgcaga acagcttttt cacaaccttt taaaattaca tatttttcca tagaacttgc
    13801 atttgatcca agatttcgat gacatctttt gtcaagaaga gagagcgatt gggcaagtca
    13861 ccttcaatca tgtgtaccat attctttatt tcgtaattca ttgcttcaga tgtattcccg
    13921 ctttcgatga attcctttgt accatctgta aataagatct ccgcacgatc agcacgcggg
    13981 taatcagcaa tcgtcacata accgttctca aaggcaacga taccgacttt tggcattttt
    14041 gcctgaaaag tcaagctgac agcagcaagt tcattttctt tgttacgcaa gatagtgacg
    14101 gattgttcgt ccacgcctgt ttcaaaagga accattgtag aggcaactac ttcaggttga
    14161 gagctcaaaa agaaacgagc gaaagataca gcatacgtac cgatatccaa cagagctcct
    14221 ccagctaatt ctggattgaa gaaccggttt ttagggtctg gttctttgta actgccgaaa
    14281 ggtgcttgga tcatttttaa agcccccagt tttcctgtat ccattattga gcgtaactgc
    14341 tgataaagtg gcatgttgaa gattgtcatc gcttctgcta agatcaaatt cttttcttct
    14401 gctaagcgca tagcgtcagc cagttctttt ttattcatcg tgatggcttt ttcacataga
    14461 acgtgtttgc ctgcttccaa tgctgccaag atatggtcga tatgctgtct atttggtaca
    14521 gcaatgtaca cgatgtcgat ttcagcatcg tctaacattt cttgatagct tccgtaagct
    14581 tttggaatat tgtaacggtg agcgaagttt tccgcttttt ctgaagttct ggaagcaact
    14641 gctgttagtt cgctcgtctc ttgattgaag ctttcggcaa actcatgtgc gatggtacca
    14701 agaccaacga ttccccattg atattttttc atctgaaaac gctccttcat agttggattg
    14761 agtttcttgt ctgtctatta ttataacaaa aatcacgaag aataaagagg agaggacttg
    14821 aaacattaaa aaaaatgaaa gcagcgattt aagaggtgct ttttgatcaa aacttaagta
    14881 gaatagttaa aaggagggga taaacgtgca tcaagtatat attgatctta ttatgagtgt
    14941 attattagaa cagtttgaaa ctgaaaaaaa ttttttagat gaatacttgc tgatcagtcc
    15001 agaacaatgg caaaaatgga aagatggcga gaaaaatctg ccatctgaag cgatgcagaa
    15061 agtaaaaaat cttttcactg attatgaatg gatgctaacg caaaagattt tgcgtcaaac
    15121 gattattttt cccgaaaaac gaaacgttgc cgttgcagag tacaagcaaa tcaaaacacg
    15181 catcgcgcaa aaatggctta gcagcaaagc agcaaaagtc gagctgatcc ctttgaaaaa
    15241 taatcagcag gctggtagat accttgactt gaaagtaagt attcaatatg atgaatgggg
    15301 ctatgatgat attttgaatt ttcgtctgcc agcagatgtt caaaaacaga tcgaaggaga
    15361 aaaaatcgaa cttctcgatt gggtgaatga aaatctggaa gaaacttacg ttcatgaggc
    15421 gaatggggaa cgtgaagaac aacgaaagga tttttcaagt gaataaattt ttaggatttg
    15481 gagcagttgt gattatgatc gcagcaatgg gctatgttat ttttagcgat gatgattcgc
    15541 atgttcaagc cggcaatcaa gcagtcgaac gaacaagcac caaaacaact gctagtaaag
    15601 aaaagaacag tcaaacaacc agcagcaagg taaaggacaa agaaacaaaa gatcgtacgt
    15661 ccaaacaatt gccagatgtt aaaacagacg attggaagct agttttggtt ggtccagatc
    15721 acaaattaga aaaagaaatt gatgagtcta ctcaattggc atcattaagt aacggatact
    15781 tgatcgacaa acgcattgct ccagaatatg aagcatttgc caaggctgct gaagcagctg
    15841 gatttccatt agtgatggtc tcagcttacc ggtctgtatc atcccaacaa caagttttcg
    15901 aacaaaatgt ccaagatgtg atgagtcgtc aaggtgtatc agaagaggag gcgacaaaga
    15961 ttactaaaga aacgattacg gagccaggat acagcgaaca ccacacaggt ctggcagtcg
    16021 atgtcgtcga tcaaaactgg tacaacagtt atccttctac tgtattggat gcaagttacg
    16081 gagatcagcc tggagctaaa tggatcgcag ataatgctgc gcagtatgga tttatcgttc
    16141 gttatccaaa aggacacgaa gatataacta agatcacata tgaaccttgg cacttacgtt
    16201 atgtcggtaa agaaaacgct gagtatatca ccaaacacca attgacgcta gaagaatatc
    16261 tgaatgaatt aaatgaaaag taggaattga tcatggcgaa aaaaaagtgg aaaaagcaaa
    16321 agcgcaagaa tatccgttgg ccagcagtgg ttgccggtgt agcagtgata ctcttagcct
    16381 ttgttttttc cttgaagaat ctgtcttctc cttttactga taatcagcaa gaaaatgaag
    16441 cgcaatccca tcagcaattt attgaacggc ttgttccgca tgcccaagaa ctacaagatg
    16501 gttatggagt gcttcctagt atcattttag gacaagctat attagaatca aattggggaa
    16561 aaagtacctt ggctagcaaa tacaataatc ttttcgggat caaggcttat ggggatcaga
    16621 agaaagtaag cctggaaaca aaggaatttg ttaacgaaga atggataact atccaaggag
    16681 acttcaaagt gtatgattca tgggaacagt ccatggatga ccatacacaa ctgttcgtcc
    16741 aaggagtaga ttggaatcct gcgctgtatg aaaaagtgat tacggccacc aactatcagg
    16801 aagccgcaca agcattgcaa gatgcaggtt atgcgacaga ccccggttat gcgcaaaaaa
    16861 tcatccaggt catcgagacg tatcaattga atcagtatga ccggagatag caaaggagaa
    16921 gtggctgatg gcagaacaat ttacgccaga aatcatgacg gaattgagaa aagatatcgt
    16981 aagagtaccg gaaatcattt tagaagcaag cgggatcaag atatttggaa aattgatccg
    17041 ttcgatcatc tttactacgg atattgcgat catacgtaat acaaatgcga atgcagtaat
    17101 tgctgtgtat ccttttacac ctcatccagc gattactaaa agcattattg aggcagctga
    17161 cattccagtt ttctccggtg taggtggtgg tttgacacaa ggtgctcgtt cttcctacat
    17221 gagcatgttc gcagaagcgc aaggttcgtt aggggtcgtt ttgaacggtc ctacgccttt
    17281 agagacagta aaagaagttt gtcgagtagt tgatattcca gttatcagta cggtaacgtc
    17341 gaaatataca gaaatagatg aaaagttagc agctggtatt tcgattatca atatcagtgc
    17401 aggtaaagaa actgcagaag cagtacgcca ttttcgtaaa aaatatcctg aacttcctat
    17461 cattgcgaca ggtggtccaa cggaagaaag tattgctgag accattcaag caggagcaaa
    17521 tgccatcaca tatacgccac caagtagcgg agagcttttt agtcgtaaaa tggaaaaata
    17581 tcgggaaact gagaaaaaga atcatgaata acaggtgaga atcaaaagac gaggaagagc
    17641 agatagtggc tcttcctcgt cttttgtact ccagtactat ttgttcagaa ttcaaaaaaa
    17701 agagaaattt ttttgttttt ttacctgtaa tgtacataag aagagttaaa accttttgtt
    17761 attattagca ttctaacaaa aaaatgtctt gatttattga agcgctttct atagaatgaa
    17821 aaacgaataa aaactaaaaa aggcacccga caatttagta gcagctaaat tgtcggggct
    17881 tgaaaagtca gcgggaactg accaatcaag ttgccatagt caatgcttgt gcatcgacgt
    17941 gtatattgta cctaatttca tacagaatta ctagttattt ttctgttttt tagggaagag
    18001 cgcacacgaa gaagcatgct tatttagcta tgtcaaggat attgggcagt tgtctgatat
    18061 ccttttttct ttgcgtaatt atcctcccat cattgaagca agaagtgttt ttagcgatga
    18121 aagaagaatc agcactatct aatttttctt gaaagtattg agttgttcat cctaggcaga
    18181 acgataaggc aagtcttggt ttttattccc cttgaaaaag atcgtttaat aaatatgttc
    18241 tgtataaaaa tacagtatat tgaaaaataa caatggaagt agaggtgcag agaatgaaga
    18301 aaagaatgag aatcatctta gctttaagtg ttatgttatt cttcttgacg gcttgtgggc
    18361 aagaagatga caaaaaaatc acgctgaaca tggcttggtg gggttcgcaa gtccgacacg
    18421 atgcaacagt aaaagtaatc gagctttatg aagaaaaaaa tccccatgtg acgatcgatt
    18481 atgaatttta tgattatgaa ggatattaca caaaattagg tacactagca gcatcagatg
    18541 atatctggga cattttccaa ctgaacaatc aatttcctca atacatcaat cagattgaac
    18601 cattagatga atatgtggag caagggataa ttgatatttc taatgcagat gaatcctatt
    18661 tagcaacgac gacttacgac gggcaattag ttggactgtc taacggggtg aattcgtttg
    18721 cgattgctta caataaaaat atatttgaac aattaaatat ccctgaacca gcttcaaatt
    18781 ggacatggga tgaatttgaa caaatcagct atcaaatcac tgaagaatta ggaatctatg
    18841 ctatttcaag attcgaagat tttatagctg gctgtattat tcaaatacca caagttgaga
    18901 aaggattgaa tttttataat cgggaagatc tatctaacag cttgggattt gaaaatccag
    18961 actatctaac agattttttc cagctgcgta aaaatctggt agacaaaggg gcccatccag
    19021 aaccaggtgg tggagctgca agtacgacgg atctgtcttt tcaggcggtt agggatagag
    19081 aagcggcaat ggtgtttcta tcaagtaacc agttgacaga aatattgtca ggggctcctg
    19141 aagatatgga gattcagttg attaatccac ctcgcagaaa agcagatgga gaatctggta
    19201 tccctttacg ttcttcgcaa atgctgagta tggcaaaaag ctcagcccat aaagaagaag
    19261 ctgctaagtt tattgatttc tttcaaaaca gtgaagaagc caatgaaatt ttaaaaggag
    19321 aacgtggtgt accaatcatg tcgaatattc gagaacagct cgctcgtgca gatgatgcaa
    19381 ctttgcaagc gacatttgat tatttagaaa tgatttccga tatggacaac ggcgaagtga
    19441 atgtctttga tagtgctttg aatccggaaa ttcaagacca atacaatctt ttagttgaaa
    19501 aagtcattta tgaagaagta acagcagaag aagcagcaga gagtctttat gagtttgcgt
    19561 caaacattct aggatagcag caggaggaag gcatgaaaaa agaaaaacta aaacggttct
    19621 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    19681 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    19741 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    19801 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    19861 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    19921 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    19981 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20041 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20101 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20161 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20221 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20281 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20341 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20401 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20461 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20521 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20581 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    20641 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    20701 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    20761 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    20821 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    20881 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    20941 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21001 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21061 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21121 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21181 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21241 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21301 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21361 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21421 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21481 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21541 tggcagaaaa aaaggtatgt tggaggaaag gaaaaatcaa acggatgtgt ttcatcatta
    21601 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    21661 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    21721 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    21781 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    21841 ttttgtatgg actgttctgg tgtatacagc atgatttttt actcactgca ttactgtatc
    21901 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    21961 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22021 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22081 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22141 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22201 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22261 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22321 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22381 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22441 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22501 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22561 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22621 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    22681 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    22741 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    22801 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    22861 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    22921 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    22981 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23041 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23101 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23161 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23221 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23281 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23341 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23401 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23461 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23521 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23581 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    23641 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    23701 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    23761 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    23821 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    23881 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    23941 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24001 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24061 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24121 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24181 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24241 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24301 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24361 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24421 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24481 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24541 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24601 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    24661 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    24721 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    24781 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    24841 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    24901 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    24961 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25021 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25081 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25141 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25201 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25261 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25321 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25381 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25441 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25501 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25561 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25621 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    25681 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    25741 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    25801 tttgagctaa taccgagggt cagctgattg aacaccgttg aaggaagagg tcgtgagcct
    25861 gccgttcatc tccgagtcac aaggaacaat ttcaaaaaaa agcttctcat gtttgttgaa
    25921 tattgttcct tgtgaccgag gagttggcag gagaacaccg tttatccata aaaaggaatg
    25981 tgacctaact tttgtcacat tccttttttt tatctttaat aaacacgaac aataaaatgt
    26041 ttttctttgt taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga
    26101 atgtagaaaa actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta
    26161 cttcagattg ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt
    26221 atgaaaaaaa ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa
    26281 acgctcgaaa aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc
    26341 cgggtatggt cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa
    26401 cattaaatgg tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag
    26461 ctactacagc aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc
    26521 tttccatgta tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta
    26581 aaactgttat agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc
    26641 gggatcgtcg gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc
    26701 tttaaagtag gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg
    26761 cacattgaaa gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat
    26821 gtcattacct atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg
    26881 tcatttattc ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa
    26941 tcttttttgg aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg
    27001 gatatccaag atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc
    27061 ggctatgatg gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg
    27121 gaccttttaa gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt
    27181 tcgatcatgg tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac
    27241 atccaccaca acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg
    27301 atcgaggagg ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt
    27361 ttaggggtag aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct
    27421 cgtcctcaca attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc
    27481 catattcgag gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca
    27541 gtaatggtca atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag
    27601 ccagactggc aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga
    27661 catatcacta tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat
    27721 atttgggatt agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat
    27781 atctggacgg aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa
    27841 gcttgggcag aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca
    27901 tcatttgata tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg
    27961 tttacacgag cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta
    28021 acaagtacgg atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt
    28081 ttacgggaag acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa
    28141 tatacagtga tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg
    28201 aaacttgcca catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca
    28261 aaaggagtag aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca
    28321 tttattgaag agtatgtatg taaacaattg gggatccgtc cacaagaaat ttctactcaa
    28381 gttctccctc gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt
    28441 attgaacgtt ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa
    28501 gaattttttg cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc
    28561 ggttcagaaa atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat
    28621 gaaaatgtca cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc
    28681 ttgcctgata ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa
    28741 aatctaacgg tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt
    28801 tatagccagc gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat
    28861 gatcttgttc agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg
    28921 ttagaagaag atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt
    28981 gattatcatc atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa
    29041 agaagagtga gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc
    29101 agatccgtta gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac
    29161 ctgattttaa aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat
    29221 taagcgtaaa tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag
    29281 ttttcgagaa aagcgtattc catctcttgt ccattgaata agatctggat acgataatga
    29341 tttttgaaac gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc
    29401 tgataaagaa aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt
    29461 cggaatgtat aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa
    29521 tcattatata gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt
    29581 tttattgcat cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa
    29641 ttctaatata ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt
    29701 ttctgataaa acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga
    29761 tttataaaaa gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt
    29821 gcttttaatg taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag
    29881 gaaaagccaa aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag
    29941 atcaagcaac tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga
    30001 ataatcaaat cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt
    30061 ttattgaaca attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg
    30121 aagtggttat ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag
    30181 gtaaagaatt catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc
    30241 catttatcaa tgaagagcat atctatttct tacaacttgc aaagccggaa gaaatcagtg
    30301 agctgaaagc ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg
    30361 gtttggtttt agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg
    30421 cggatgaagt aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg
    30481 acaaagacgt ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata
    30541 accgtctatc ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata
    30601 tgtaacctat aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca
    30661 tcgtatggga tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact
    30721 tgctgatgca gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc
    30781 aaatgtaaat atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga
    30841 aatttgcggt catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc
    30901 gcgagatcat gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat
    30961 ttgatggggt tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga
    31021 ttgctcggtt ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag
    31081 tattcggtac atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac
    31141 tgagacgaaa tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca
    31201 agacggcctt tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg
    31261 gggaaggaaa ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga
    31321 ttgcctttac ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga
    31381 acaaagcagg gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc
    31441 taggatcaga agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga
    31501 ccgtatagat gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct
    31561 atgccgaatg gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa
    31621 gaatgcctaa ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct
    31681 ataaaaattc taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag
    31741 gccctggaga aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag
    31801 cagaaagtca caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg
    31861 gcggaatcat cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat
    31921 tgaggttcgg cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag
    31981 gtatcggcgg atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg
    32041 atccttgtta tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca
    32101 aacatatcca gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa
    32161 aaacaggtcg cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag
    32221 aagaacagca acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg
    32281 aagcgtgttt ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg
    32341 gagcagcagg acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta
    32401 tattgacatt agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt
    32461 tatcggaatc acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc
    32521 aagaactatt tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg
    32581 ggcaatatcg gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag
    32641 ctgaagatgc accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc
    32701 agcagttagc tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc
    32761 tgcagcaacc gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac
    32821 aaaccaatac agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga
    32881 ataaagcgtt agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa
    32941 tcggaggaca aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc
    33001 ccttggcgat caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg
    33061 aattatcgac ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta
    33121 tttcaggaaa tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa
    33181 tgattggtat ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa
    33241 aatcagggga tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat
    33301 tacaaaagat gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg
    33361 aaaagagaaa ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc
    33421 atgactgttc agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc
    33481 tagggatatc tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa
    33541 gatttgtgtt gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg
    33601 ctgctgtttt agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg
    33661 gtcagatcaa aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc
    33721 ttatgaagtg aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga
    33781 cgccgctagt gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc
    33841 aggtatcgtg tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc
    33901 tgaggtattc aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca
    33961 tgtacgttat gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa
    34021 atttcatgat cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct
    34081 tagaaaagag ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt
    34141 gatgcatttg atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt
    34201 gaatgaagtc aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc
    34261 tttggaccca aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt
    34321 gattgcctcg gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca
    34381 gcctggtgag atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga
    34441 cacgcagctt tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat
    34501 cgcagggatc aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc
    34561 gccagtaaaa gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg
    34621 ttatgcggaa gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc
    34681 ccgaacattt atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc
    34741 tgctgtacga ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg
    34801 gggtacgaca agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca
    34861 tgtgaggatt gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac
    34921 gcgtaaagaa ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc
    34981 agattcttta ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga
    35041 tgctccgtat tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata
    35101 tgactatgaa gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca
    35161 gaccaagaag ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg
    35221 tcgaagctgg atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt
    35281 taggtatgat gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat
    35341 taaaagagcc agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa
    35401 tcgaagcaga caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata
    35461 tcttagcaca aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg
    35521 atccgttacg tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata
    35581 tggcgctgat tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg
    35641 atctagccgg atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga
    35701 tcaacccagg agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact
    35761 ctcttgttcg caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag
    35821 aattgaaaaa aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct
    35881 tgcttccgct gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt
    35941 ttgtagaaaa tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct
    36001 catggccgat tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg
    36061 agatgtatga aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag
    36121 taaaagaaat agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag
    36181 tgatcgccaa agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt
    36241 ttttgcttct ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg
    36301 attggaatct tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg
    36361 tgcaactgcg ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa
    36421 agagtatgaa gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt
    36481 agctggatat atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat
    36541 caatatccat ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt
    36601 tgaagctgga gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac
    36661 tggaccaatc atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt
    36721 agaagaaaaa atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat
    36781 tgaaaatgga ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg
    36841 attgtagcat tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga
    36901 acaaagcatg cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg
    36961 tttcctgaaa tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt
    37021 ttaggaagac gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca
    37081 atcgactttg tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc
    37141 gaaatagcag aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca
    37201 gcaaaaaacc atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt
    37261 tcagaattac gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa
    37321 gtctttcgtc atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg
    37381 gaagaaaaag aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac
    37441 ggtgaaaata gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg
    37501 atcgcagcag caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca
    37561 gatgctgcgc tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac
    37621 atgaatcctt gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat
    37681 gaagcagatc ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg
    37741 ccaacagcag aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca
    37801 caagaagctt tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt
    37861 gcgcaggaag aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta
    37921 caagatcaag atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag
    37981 ccgactccag cagaagcaaa agcattagtg tttgcttgga gagctgtcaa acatgtgaaa
    38041 tctaatgcga tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg
    38101 aatcgagtcg gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca
    38161 gttttagcta gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat
    38221 ggtataaaag caatcattca gccaggagga agcatcaaag accaagattc tattgatatg
    38281 gcgaataaat acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga
    38341 ggagtcggat aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt
    38401 aaaaaacttt tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg
    38461 atgacaaaag atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc
    38521 cattttgtta agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta
    38581 aatgggatcg tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca
    38641 gcagctctga tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc
    38701 ccgacagcta aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa
    38761 aatggtgcgc caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg
    38821 gctaccacag tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt
    38881 ggggatagta gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt
    38941 ttggcgtttg tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg
    39001 gcatttgata acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg
    39061 caaatttcaa aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg
    39121 ggaatgaaag aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg
    39181 aaggaaggac caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc
    39241 gttctttctc ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt
    39301 acagtagagt taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc
    39361 ggttaccccg aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt
    39421 tctgtttact atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt
    39481 ctttatttag tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc
    39541 gcgttaatgg ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct
    39601 gccaatagcg aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa
    39661 acataatcgg aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg
    39721 ttgtgtatat aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca
    39781 gaaagcagct gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa
    39841 agtgattgag acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg
    39901 aaacggtgaa tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga
    39961 agatcttttt attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa
    40021 aaaacatatc attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga
    40081 tgctttactt cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt
    40141 tgatgaattg gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat
    40201 gcaaatcgaa ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt
    40261 tggcttgaaa catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc
    40321 aagtatcgat catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca
    40381 agcatggtca ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt
    40441 ccctgaatta aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg
    40501 cttggcttct gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat
    40561 gaacgtaggg cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga
    40621 ttggtacgaa ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg
    40681 tcaaaagggc tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat
    40741 attctgtctt tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt
    40801 tatttttgtt tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt
    40861 tcttaaataa ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg
    40921 aaatacgata gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca
    40981 gataatcgat gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac
    41041 ttaatgcaac ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt
    41101 tgataaaatc caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg
    41161 gcagagcagt taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg
    41221 cgctgattgc taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct
    41281 tttctttcag gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg
    41341 ttcgagttgg tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt
    41401 tcgataatga agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt
    41461 tcgcttcttg aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt
    41521 caagtatcat ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag
    41581 gaccaatggc gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt
    41641 gggctacctg ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt
    41701 tgatgaatcg agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact
    41761 tcaaataatc actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta
    41821 ataatacggt catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg
    41881 tttcactgat cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt
    41941 cgaaaacaag atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg
    42001 ctttttctat tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg
    42061 cctgtacctg gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga
    42121 gacgagtcca aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag
    42181 gcatttggat atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca
    42241 tctcaccatc tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta
    42301 tttgttcccg caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca
    42361 tgtttgtttc ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct
    42421 tctcggtatt tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga
    42481 cgcttgttta cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct
    42541 aactctttac gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact
    42601 ttgaattgga tcatctcatc aattttgcat gtggtaatac gaataaatcc tttgtgacgg
    42661 acgtacttcg ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa
    42721 atagaaacag gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat
    42781 cttattttta agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc
    42841 cagtcttgtc ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc
    42901 agagcttctt caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa
    42961 ataaaatggg gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat
    43021 tgtttcaaag cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta
    43081 gcggtaagtg tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct
    43141 gaagaaagtt gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct
    43201 aaaaagcgac gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact
    43261 attgttaata gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc
    43321 tccacacgaa caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga
    43381 ttgatgatct cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg
    43441 ttttgataat cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct
    43501 ttaagtgtat ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata
    43561 ttttcctcat tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc
    43621 aaaacttttt gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc
    43681 tgttgataaa tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca
    43741 gcaaatagaa gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt
    43801 gtcatgatac ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga
    43861 aaagacagtc tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc
    43921 aaaataacga gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac
    43981 acaattgacg attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac
    44041 aatgatcaaa tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg
    44101 agtcatttca tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa
    44161 atatcgtttc atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac
    44221 aatagtcatc ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt
    44281 gattgattcc aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc
    44341 aagttctagg attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa
    44401 acccgcccga tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg
    44461 atcattcgtt tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc
    44521 ttttaataca gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt
    44581 ataggcaatg aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg
    44641 gtagagaact tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt
    44701 ccgccagcta ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc
    44761 ctttgtatta ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa
    44821 taaaacacgt ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata
    44881 aaagggaaaa tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat
    44941 ccaagacaaa aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt
    45001 aacgctttct ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa
    45061 aacaaattta gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt
    45121 gcgtgtggca attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg
    45181 accaaatcag cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa
    45241 ctgggttata aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa
    45301 atagaaaata tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg
    45361 gctttaactg atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat
    45421 cgtttactga tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta
    45481 ggtgtatcgc aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca
    45541 ttcacgattg aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac
    45601 ggagtgatgt ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga
    45661 cagacgagtt tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg
    45721 atggataatc tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca
    45781 tatgatccaa taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa
    45841 agtaaacctt tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc
    45901 attgccgggg aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg
    45961 attgaaatga tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact
    46021 tatgataatg gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa
    46081 gataattatc aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat
    46141 taagaggaac caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg
    46201 cggattgtat tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag
    46261 attgcgtgaa tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag
    46321 cgggaaaatc aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg
    46381 gggaaatctt ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa
    46441 aaggcatcgt catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga
    46501 atatttttct cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa
    46561 agaaaacata tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat
    46621 cccagattgg cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg
    46681 tccgtctgtt gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt
    46741 tgctgagatt gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata
    46801 agctgaatga aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga
    46861 ttgagacact agaaaatgaa atgatcagcg aagagcatat tattcgcgga atggtgggca
    46921 gagatttagt tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag
    46981 tagaagactg gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca
    47041 acttgcattt gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa
    47101 ctgaattcgc tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat
    47161 taaaggaagg ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg
    47221 cttatgtctc ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa
    47281 acgtgacgct gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag
    47341 aagtaactga ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc
    47401 aaattgtcag tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga
    47461 tgacacaacc cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa
    47521 aatatgagat ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta
    47581 tctcttctga acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag
    47641 gacgtattac aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa
    47701 tgacgaaaga agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag
    47761 aaaaaactaa atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc
    47821 attttagcgg gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca
    47881 ttgaatataa ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg
    47941 ttattagttg tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt
    48001 ggtgcaattg cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc
    48061 gcatgtcttg cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca
    48121 gggataccgg cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa
    48181 gttgttctag gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg
    48241 ggttatttac cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga
    48301 atgatcatca cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat
    48361 catttatttg aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc
    48421 gttgtactag gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc
    48481 attcttggtt tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga
    48541 cagatttatg cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag
    48601 cgaatcacgt tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt
    48661 ttagctgctc gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca
    48721 atggcggcag tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt
    48781 gcgattgtcg gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc
    48841 ggggtagact ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac
    48901 ttgtataata agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg
    48961 tgacaagaca tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg
    49021 tatagactaa aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg
    49081 atttttttgc ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta
    49141 ataatgttcg tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga
    49201 ttacgaagat attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga
    49261 tacaacggtc acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca
    49321 gacgattatt gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat
    49381 gcatcggttt gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt
    49441 gattacctcg atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc
    49501 agaagaatcg ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc
    49561 agttattgat atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg
    49621 taatgttggt acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa
    49681 agtcgggatt ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg
    49741 aggctggcaa ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc
    49801 tgacggtggg atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat
    49861 ggtcatgatt ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga
    49921 aaatggaatc gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa
    49981 acgaaatgtt gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact
    50041 agtggaaatg caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga
    50101 agccattcga aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat
    50161 ctaaaaaata aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag
    50221 aacagccaaa caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat
    50281 acaagctttt gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc
    50341 ccttatgttt tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa
    50401 aaaatagaca atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg
    50461 atttatcgtc aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct
    50521 ggaagtcaac ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc
    50581 gttgtccatg cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc
    50641 ggatggtttg ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg
    50701 caaatgatct ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca
    50761 aagcagatcc aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt
    50821 ttgccgaatt ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa
    50881 atggatcttt taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa
    50941 tttgaattgg cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc
    51001 tttttgattt tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct
    51061 tttttttacc gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg
    51121 gatgaagaag ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa
    51181 gcagtgctta tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga
    51241 cgagaaaagc tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta
    51301 tttacggatc tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg
    51361 gataatgtgt tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt
    51421 tgggtgatag gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag
    51481 ttttcgatga atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat
    51541 ttgaaaatgc aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg
    51601 ggagattcac aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt
    51661 acttgggatg ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt
    51721 ggaattgcac caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt
    51781 aggctggatg aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg
    51841 ttaacagaat aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt
    51901 tcaagctgtt tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca
    51961 ttatttgtac gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata
    52021 acaccaacgt ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat
    52081 gcagatgaaa tataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg
    52141 cggattggca taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata
    52201 aatccatgat aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt
    52261 caatcgcaga atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa
    52321 caaaaaagca ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt
    52381 atatcaagca cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc
    52441 cgatgatgaa ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg
    52501 ttcaacagcg ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga
    52561 ggcaagtcag ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta
    52621 tcgggatgta cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc
    52681 tcgaggacat gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat
    52741 tatcatcggt gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa
    52801 caagaaaaat gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta
    52861 cgaagcaatc aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa
    52921 cgggtatgcg atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa
    52981 agctgtcgca gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata
    53041 tactgtatct aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga
    53101 aacactgact tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg
    53161 tacaaaagac acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata
    53221 tttgacggaa aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa
    53281 agaagaaatc aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga
    53341 tttcttgaaa aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta
    53401 tgaagcgaag gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat
    53461 gccttagcac ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg
    53521 aaaaacggag gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga
    53581 gtattcgata ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta
    53641 gaaggatttc gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac
    53701 gaagtagtag ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc
    53761 atcacaattc gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat
    53821 ttagaaggtt tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat
    53881 gatgcaaaag gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa
    53941 cacatgaaac tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct
    54001 ttggataaag cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca
    54061 atggtgcgtg aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa
    54121 atcattgatc ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa
    54181 aaaactggtc gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa
    54241 gtagtttctg agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt
    54301 tctgcacctg atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca
    54361 aaagatatcg aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac
    54421 aaagtcatgg cttatcaatt taaatt
//
LOCUS       DAFFFQ010000011        48208 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000011
VERSION     DAFFFQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.011"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LQB69_000741"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LQB69_000741"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL3524927.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LQB69_000742"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LQB69_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL3524928.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LQB69_000743"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LQB69_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL3524929.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LQB69_000744"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LQB69_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL3524930.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LQB69_000745"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LQB69_000745"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL3524931.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LQB69_000746"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LQB69_000746"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL3524932.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LQB69_000747"
     CDS             complement(8053..8523)
                     /locus_tag="LQB69_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL3524933.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LQB69_000748"
     CDS             complement(8569..8994)
                     /locus_tag="LQB69_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL3524934.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LQB69_000749"
     CDS             complement(9061..9330)
                     /locus_tag="LQB69_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL3524935.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="LQB69_000750"
     CDS             complement(9341..10936)
                     /locus_tag="LQB69_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3524936.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LQB69_000751"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LQB69_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL3524937.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="LQB69_000752"
     CDS             complement(14489..15049)
                     /locus_tag="LQB69_000752"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL3524938.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="LQB69_000753"
     CDS             15402..16376
                     /locus_tag="LQB69_000753"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL3524939.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="LQB69_000754"
     CDS             16546..17946
                     /locus_tag="LQB69_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL3524940.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="LQB69_000755"
     CDS             complement(18024..18746)
                     /locus_tag="LQB69_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524941.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="LQB69_000756"
     CDS             complement(18825..19331)
                     /locus_tag="LQB69_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3524942.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="LQB69_000757"
     CDS             19454..21253
                     /locus_tag="LQB69_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL3524943.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="LQB69_000758"
     CDS             complement(21321..22895)
                     /locus_tag="LQB69_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL3524944.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LQB69_000759"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="LQB69_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL3524945.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LQB69_000760"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="LQB69_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL3524946.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="LQB69_000761"
     CDS             complement(24672..25310)
                     /locus_tag="LQB69_000761"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL3524947.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="LQB69_000762"
     CDS             complement(25311..25961)
                     /locus_tag="LQB69_000762"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL3524948.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LQB69_000763"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="LQB69_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL3524949.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LQB69_000764"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="LQB69_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL3524950.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="LQB69_000765"
     CDS             complement(29104..29844)
                     /locus_tag="LQB69_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL3524951.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LQB69_000766"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="LQB69_000766"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL3524952.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="LQB69_000767"
     CDS             complement(31215..32153)
                     /locus_tag="LQB69_000767"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL3524953.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="LQB69_000768"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="LQB69_000768"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL3524954.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LQB69_000769"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="LQB69_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL3524955.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="LQB69_000770"
     CDS             complement(35313..35618)
                     /locus_tag="LQB69_000770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL3524956.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LQB69_000771"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="LQB69_000771"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL3524957.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="LQB69_000772"
     CDS             complement(36724..37008)
                     /locus_tag="LQB69_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524958.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="LQB69_000773"
     CDS             complement(37026..37916)
                     /locus_tag="LQB69_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL3524959.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="LQB69_000774"
     CDS             complement(37936..38154)
                     /locus_tag="LQB69_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL3524960.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="LQB69_000775"
     CDS             complement(38124..39824)
                     /locus_tag="LQB69_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL3524961.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40090..40863
                     /locus_tag="LQB69_000776"
     CDS             40090..40863
                     /locus_tag="LQB69_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524962.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="LQB69_000777"
     CDS             complement(40944..41513)
                     /gene="thiT"
                     /locus_tag="LQB69_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL3524963.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41579..41681)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41687..42658)
                     /locus_tag="LQB69_000778"
     CDS             complement(41687..42658)
                     /locus_tag="LQB69_000778"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL3524964.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="LQB69_000779"
     CDS             complement(42752..44125)
                     /gene="glmU"
                     /locus_tag="LQB69_000779"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL3524965.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44628..45446)
                     /gene="purR"
                     /locus_tag="LQB69_000780"
     CDS             complement(44628..45446)
                     /gene="purR"
                     /locus_tag="LQB69_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL3524966.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45613..46437)
                     /locus_tag="LQB69_000781"
     CDS             complement(45613..46437)
                     /locus_tag="LQB69_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3524967.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46410..47090)
                     /locus_tag="LQB69_000782"
     CDS             complement(46410..47090)
                     /locus_tag="LQB69_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3524968.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47113..48051)
                     /locus_tag="LQB69_000783"
     CDS             complement(47113..48051)
                     /locus_tag="LQB69_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL3524969.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
BASE COUNT        13200 a        10455 c         8696 g        15857 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttagtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctga ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcgcgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgt gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacatccttt aaataaaaaa tacaaaaatc acaaaaatta
    39961 attgaaaatg agagtgtttt caatgaaaac gtgattttgt aatggaattt taaaataaaa
    40021 gaaacaagct tttttaatgc aaatgttgta aaataaaaga gattaagagg aaataagagg
    40081 aggaacatga tgactgagtc agtttatgta catattgata ccacaagtaa tgctgtttta
    40141 acaaaagggc tgactgctac tgactttgca aacagtatcg tgcattttcc tcaaaacttg
    40201 ctcttgcttg atccatctgc ttctgctgga gaatatgaat cccatacagg gctgaaagta
    40261 atccgtggta cagaaaatat tcagcgtttc ttttcctctt ctcgtaacag gcgtggattc
    40321 gatgaattga agtggatcga ctttactgat ttaacgatgc tgaaagaatt gacaccttta
    40381 gaaatttcag aattattata ttttggtcat atgaaaacac atcttcattc gccgtttttt
    40441 tataaactac agaataattt tgtatacttc gatcttaatg atcagctgaa tcggatttat
    40501 tatcgctatt tagatgaatt ttataggatt tttgctagta aattaagttc gatcttgtca
    40561 gataagatca atgtaaaaaa atcttttttc aaaaaagaaa cacctgttga aaaaatcagt
    40621 gtggaattat tgaaagaaat gcgtaatttg atgcaggaag ggatcatttt ttcttttgat
    40681 caagttggtt tgatcgatgg taaatatcgt atcccaatct atgtgattga agattatatt
    40741 tggaaattaa aaggcacccg gtatcgagaa gaggaaatca tcgctacttt aatctactct
    40801 gtcaccgatc gacactggca attggtagaa gatgccgatc tttcttatct gccattgaag
    40861 tagatctatt tcttatcaaa aaataaaaag gaaacaagaa actaggacat gattctgtct
    40921 caagtttctt gcttcttttt tatttacagc tcctgtcgtt tatctggcac aaaaatcgaa
    40981 ggagtcgttt gttggatccc taataaaacg atgatcgtta caatagctgt actcaacccg
    41041 ctggcaccat tcattacgaa agaaaagagc caagaattca tcccccatag tgcatacgct
    41101 ccccagaaaa ccactcctgc aataaaatgc caaaaatatc tggaaagtgc gccaatcaat
    41161 gcaccaaaca cgatgctttt tctaatttgt ttcgtattat cgtctacata gcctttttgg
    41221 acatttgaag ccatgagccc tgctgcgcct gcaaaagtat aggcaatcgg gtactcaatc
    41281 aacacttgaa cgacacttaa ataataaact tgtcccaacg gaaaatgtag gatgccccaa
    41341 atgaaacctg ccagcatccc tgcctttgtc cctcttctta acgcaaaaag agtcaatgga
    41401 atctgcccta atgaaataga aaaactgctg ccgatctgga tcggaatcaa cgacaacacc
    41461 atcgctaacg ctgcaacaat cgttccttct acccaaactc tattttttcc cataaaaaaa
    41521 cttcctcctt tgaataaaaa agtcacaaag gaggaagcag ctttcgaaaa tagtcaaaca
    41581 gctgtttatt cacaattcct acgctcgcat aaccgataca ggtacgaagg gtttagaaat
    41641 agattcaatc tcagcccaat tggactcccc tttgtgagtt tatttattat ttattttttg
    41701 tttcaaacaa tggactaact ggtttgtttt catggatacg tttgattgca tctcccatta
    41761 gtccgccaac acttacttca tcgattttgt cgattttacg atcttctgga agatagatcg
    41821 agtctgtaac aactagacgc tcgatagctg aatcttctat tctttgtaaa gcagggccgg
    41881 ataatactgg atgtgtacat gaagcataca cactagttgc accagcttct ttcaaagcat
    41941 tggcagccaa tgtgatcgta cctgctgtat cgatcatatc atcgatcagt acacaagttt
    42001 tgcctttgac ttgtccaatg atattcatta cttctgcaac atttgctttt ggacggcgtt
    42061 tgtctatgat cgcaatcgga gatttcaaaa attccgctaa tttacgtgcc cgtgtgacac
    42121 caccatggtc tggtgaaaca acgacaacat catcgccttt gattcctttt tctaagaaat
    42181 aattggcaat aagtggcgca cccatcaaat gatcgactgg aatatcaaag aaaccttgga
    42241 tttggactgc atgaaggtcc aaagtcagca ttcttgttgc accagctttt tgaatcatgt
    42301 ttgctactaa tttcgctgtg attggttccc gtgcacgtgc tttacggtct tgacgcgcat
    42361 agccataata aggcatgact acgttgattg tttttgcact tgctcgtttc agtgcgtcaa
    42421 tcatgatcag caactccatc aagttatcat tgactgggct gctcgttgat tgaataacat
    42481 agacatgcga tccacgaata ctttcttcaa tgtttacttg aatttctcca tcactaaatt
    42541 gagtaacaga tgattttcct aactcgacgc cgactgctgc tgcaattttc tctgctaatg
    42601 ggcgattgga atttaacgca aaaattttta atcttgggtc aaagtaatgt tttgacatgg
    42661 ggacctccac tttctttttc acaactctag aattctacct aataaatagt agtcatattt
    42721 tttaaataaa tcaagtgatt ttgcaaaagt cttagtttag ataagggagt ttttttgcat
    42781 atccttcttt attgacttgt cttgctctcg caattgccag cgcatattct ggaatattgt
    42841 ctgtaattgt tgacccggca gcaatcgaac tgtttttagc tacctcgaca ggtccgatga
    42901 tattcgtgct agaaccaata aagctgtggt cgccgacagt cgtatgatgt ttgttctttc
    42961 catcatagtt gacgaagaca acaccacatc cgacattgat ttcttctccc agcgtagcat
    43021 caccaacata agtcaagtga ccgactttag ttcctttgcc gatttgagca tttttcactt
    43081 ccacaaagtt tccgatatgg acgttttcgc caatttcagc ttttggtcgt aaatgtgcat
    43141 aaggtcccac atctgcatgt ttcttgacat ggctgctttc aatgactgag ttttcaatga
    43201 cgacgtgatc ctcaatccta ctgtctacga tttttgaatg tgacccgatg acacaatcag
    43261 aaccaatgac tgtatttcct tggatttgta cacctgcttc gatcaatgtg tctggaccga
    43321 tttctacgcc tgcatcgatg tatgtcgttg ctgaatcgac aaagcttacg ccattgacca
    43381 tatgcatctg gttaattcgc tttctcatga tctcatttgc tttagctaac gcgatccgat
    43441 cattgactcc cattgattct tcaaaatcat ccgtttgata agcagcaact gtatggcctt
    43501 cttcttttaa gatttcgatg atatctgtta gatagtattc accttgagca ttgtttgttc
    43561 caactttgct taacgcatcg aacaaagctt cgttgtcaaa acagtaagtt cctgtattga
    43621 tttcttgtac aagagcttct tctggtgttg catctttttg ttccacgatt ttttctacga
    43681 tcccaatatg gtcacggatg attcgtccat atcctgttgg gttttctgct tgtgccgtca
    43741 agatcgttgc acttgcattt tttccttggt gatattcaaa aagattgttc agcgtttcag
    43801 tagtcaataa tggtgtatcg ccactgataa ctaacgttgt tccttttttt ccttccaaga
    43861 aagaagcagc ttgcaaaaca gcatgtcctg ttcccaattg ttctgcttgt aacgcatatt
    43921 tactgcgttc tcctagttgt gctttaactt gttctgctcc gtgtccaacg atcgtaatca
    43981 cttcatctgg tttcgtttca ctgacccgat taatgatatg ttctaccata ggtttaccag
    44041 aaacgggatg taaaacttta tataattttg acttcatgcg ggtacctttc cctgccgcca
    44101 aaataattgc ataacgtgcg tccaactgcg tcactcctaa atatagtttg ctgatttcag
    44161 tttatcccag tatacccatt ttttcaacaa aacaagtgat tttcttttat cccagcgttt
    44221 tctggataaa aaaatagata gaccataacg ggcctatcta tttggtaacc ggtgctctcc
    44281 tgccaactcc tcggtcacaa gtcacactac tcataaaaca tgaaaagctg ttttttgaaa
    44341 tcgtttcttg tgacttagag ttaaacggta ggcttacaac ctcttcatta aacgtttttc
    44401 aatcagctga ccttcggtat tagctcaata aacggcaaaa tatgaggagc aatttttccg
    44461 tttattgagc taatactcag gacaggacgc tgatttcaca acctctagat aaacggtgtt
    44521 caaaagccag tttctcggta ttaagccaaa tttatgaaaa aatgtggaaa gccattttca
    44581 caaaattttt cttactactc gaaactaagc ggctttttca caacctctta ttctgaaaaa
    44641 taatttcctg gcacaaccgt aatatttttc gtttgagtat ctacatcttt gacataaagc
    44701 agtgacgtat aatcatcgat ggcgcgatga ccgttgaatt ttgattcggc aaatacagtg
    44761 atgccaacaa gttctgattc gaattcttca atcaagctct tcattccgtt gactgttccg
    44821 ccgcctttca tgaaatcatc cactacaagg acacgtgaac cacgtttcaa acttctctta
    44881 gacagttcca tcttttcaat tctttcagaa gatccagaca catagttgac acttactgtt
    44941 gatccttctg ttatttttga gtcccggcgt acaataacga atggcgcatt cagataatag
    45001 gacacagctt gggcaatagg tacgcctttt gtggctacag tcattacagc atcgattttt
    45061 tcaccaaggt atttagaagc aatgattctt ccaacttgac gcagcagttc aggttctcct
    45121 aacaaatcag agagataaac atatcctcct ggaagcagac ggtcttgttc tgatagacgt
    45181 tctgctagag attcaatata ttttttcgcc tcttcatatg aaatttcagg gatgaacaat
    45241 acaccacctg cagcacccgg gattgtttcc aaggttccat accctcgttc tttaaatgtt
    45301 ttcttgatga ttcccaaatc ttcactgata gaagatttag cagattcata acgttcggca
    45361 aagcttgtta aggaaatcaa ttcatgcggg tgattcaata aatactgtgt catgtcaact
    45421 agacgttcac tgcgacgtac tttcacctat tccaactcct tttaagaaat tttatcgttt
    45481 tacattatat aagtttttct tcaaaaattc gagcatttta tgaaaaaata agaaaatagt
    45541 tcggtattta ttaaaataaa aagaagccta taaaaaattt ttcgttttta taaggcttct
    45601 tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt
    45661 tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag
    45721 ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc
    45781 aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat
    45841 tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat
    45901 cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc
    45961 atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac
    46021 gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag
    46081 ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag
    46141 cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt
    46201 tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg
    46261 attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc
    46321 agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc
    46381 aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga
    46441 gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga
    46501 tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga
    46561 taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag
    46621 tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat
    46681 tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca
    46741 tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa
    46801 cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga
    46861 atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct
    46921 tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt
    46981 tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca
    47041 ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct
    47101 ccattctaaa acttaattga tactttcttt taaagctttc agattttttt ccatgacaga
    47161 aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac
    47221 ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc
    47281 ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg
    47341 acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta
    47401 ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc
    47461 ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc
    47521 attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt
    47581 tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga
    47641 atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc
    47701 gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga
    47761 atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc
    47821 tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag
    47881 tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt
    47941 cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc
    48001 aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct
    48061 cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg tatcaaaata
    48121 aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta
    48181 aatcgtaaaa tggatgagtt gctgctca
//
LOCUS       DAFFFQ010000012        47996 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000012
VERSION     DAFFFQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.012"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LQB69_000784"
     CDS             complement(22..1806)
                     /gene="pyk"
                     /locus_tag="LQB69_000784"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL3524970.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LQB69_000785"
     CDS             complement(1880..2842)
                     /gene="pfkA"
                     /locus_tag="LQB69_000785"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL3524971.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LQB69_000786"
     CDS             complement(3010..6324)
                     /gene="dnaE"
                     /locus_tag="LQB69_000786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL3524972.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            6555..6740
                     /locus_tag="LQB69_000787"
     CDS             6555..6740
                     /locus_tag="LQB69_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL3524973.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(6802..7998)
                     /locus_tag="LQB69_000788"
     CDS             complement(6802..7998)
                     /locus_tag="LQB69_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL3524974.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(8168..9604)
                     /locus_tag="LQB69_000789"
     CDS             complement(8168..9604)
                     /locus_tag="LQB69_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL3524975.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            9897..10706
                     /locus_tag="LQB69_000790"
     CDS             9897..10706
                     /locus_tag="LQB69_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3524976.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(10892..13045)
                     /locus_tag="LQB69_000791"
     CDS             complement(10892..13045)
                     /locus_tag="LQB69_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL3524977.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(13440..14288)
                     /locus_tag="LQB69_000792"
     CDS             complement(13440..14288)
                     /locus_tag="LQB69_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3524978.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LQB69_000793"
     CDS             complement(14512..16452)
                     /gene="thrS"
                     /locus_tag="LQB69_000793"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL3524979.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(16789..16968)
                     /locus_tag="LQB69_000794"
     CDS             complement(16789..16968)
                     /locus_tag="LQB69_000794"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL3524980.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LQB69_000795"
     CDS             complement(17015..18838)
                     /gene="cadA"
                     /locus_tag="LQB69_000795"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL3524981.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            19116..21395
                     /locus_tag="LQB69_000796"
     CDS             19116..21395
                     /locus_tag="LQB69_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3524982.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(21488..22135)
                     /locus_tag="LQB69_000797"
     CDS             complement(21488..22135)
                     /locus_tag="LQB69_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3524983.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(22314..23033)
                     /locus_tag="LQB69_000798"
     CDS             complement(22314..23033)
                     /locus_tag="LQB69_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3524984.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(23164..24315)
                     /locus_tag="LQB69_000799"
     CDS             complement(23164..24315)
                     /locus_tag="LQB69_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL3524985.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(24532..25272)
                     /locus_tag="LQB69_000800"
     CDS             complement(24532..25272)
                     /locus_tag="LQB69_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3524986.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LQB69_000801"
     CDS             complement(25276..25599)
                     /gene="rsfS"
                     /locus_tag="LQB69_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL3524987.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LQB69_000802"
     CDS             complement(25656..26249)
                     /gene="yqeK"
                     /locus_tag="LQB69_000802"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL3524988.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(26233..26883)
                     /locus_tag="LQB69_000803"
     CDS             complement(26233..26883)
                     /locus_tag="LQB69_000803"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL3524989.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LQB69_000804"
     CDS             complement(26893..27204)
                     /gene="yhbY"
                     /locus_tag="LQB69_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL3524990.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LQB69_000805"
     CDS             complement(27220..28329)
                     /gene="yqeH"
                     /locus_tag="LQB69_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL3524991.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(28326..28853)
                     /locus_tag="LQB69_000806"
     CDS             complement(28326..28853)
                     /locus_tag="LQB69_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL3524992.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(29033..29818)
                     /locus_tag="LQB69_000807"
     CDS             complement(29033..29818)
                     /locus_tag="LQB69_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL3524993.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(29811..30680)
                     /locus_tag="LQB69_000808"
     CDS             complement(29811..30680)
                     /locus_tag="LQB69_000808"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL3524994.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(30681..32060)
                     /locus_tag="LQB69_000809"
     CDS             complement(30681..32060)
                     /locus_tag="LQB69_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL3524995.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LQB69_000810"
     CDS             complement(32068..32487)
                     /gene="fabZ"
                     /locus_tag="LQB69_000810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL3524996.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LQB69_000811"
     CDS             complement(32512..32988)
                     /gene="accB"
                     /locus_tag="LQB69_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL3524997.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LQB69_000812"
     CDS             complement(32991..34226)
                     /gene="fabF"
                     /locus_tag="LQB69_000812"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL3524998.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LQB69_000813"
     CDS             complement(34245..34982)
                     /gene="fabG"
                     /locus_tag="LQB69_000813"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL3524999.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LQB69_000814"
     CDS             complement(34994..35911)
                     /gene="fabD"
                     /locus_tag="LQB69_000814"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL3525000.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(36018..36242)
                     /locus_tag="LQB69_000815"
     CDS             complement(36018..36242)
                     /locus_tag="LQB69_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL3525001.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(36295..37257)
                     /locus_tag="LQB69_000816"
     CDS             complement(36295..37257)
                     /locus_tag="LQB69_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL3525002.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(37267..37707)
                     /locus_tag="LQB69_000817"
     CDS             complement(37267..37707)
                     /locus_tag="LQB69_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3525003.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            38013..38978
                     /locus_tag="LQB69_000818"
     CDS             38013..38978
                     /locus_tag="LQB69_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL3525004.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(39035..39265)
                     /locus_tag="LQB69_000819"
     CDS             complement(39035..39265)
                     /locus_tag="LQB69_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL3525005.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(39351..40238)
                     /locus_tag="LQB69_000820"
     CDS             complement(39351..40238)
                     /locus_tag="LQB69_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL3525006.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(40327..41550)
                     /locus_tag="LQB69_000821"
     CDS             complement(40327..41550)
                     /locus_tag="LQB69_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3525007.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(41567..42613)
                     /locus_tag="LQB69_000822"
     CDS             complement(41567..42613)
                     /locus_tag="LQB69_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3525008.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LQB69_000823"
     CDS             complement(42777..44504)
                     /gene="ptsP"
                     /locus_tag="LQB69_000823"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL3525009.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(44504..44770)
                     /locus_tag="LQB69_000824"
     CDS             complement(44504..44770)
                     /locus_tag="LQB69_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL3525010.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            45249..47483
                     /locus_tag="LQB69_000825"
     CDS             45249..47483
                     /locus_tag="LQB69_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3525011.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQKAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            47631..47951
                     /locus_tag="LQB69_000826"
     CDS             47631..47951
                     /locus_tag="LQB69_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL3525012.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        13691 a        10309 c         8401 g        15595 t
ORIGIN      
        1 tttatttttt attaagctaa attagatagc tgttgtttcg ccacggtaaa cgatgccgcg
       61 acgtgaatca acggtaatca attcaccttc tgtaattgtt gttgttgcgc ctttagcacc
      121 aacgattaca ggaatatctt gtgcaattcc tacaactgca gcgtgagaag tcaatccgcc
      181 ttcttcaacg ataacagcag ctgctttttc gattgcaggc atatattctt tgtcagttga
      241 tggtacaact agaaccattc cgtcttttgc tttttttaca gcttcttcag cagatgaagc
      301 aataactgca tttgcaacga cagatttttg tccaacgcct tgagcttcta ataatttaga
      361 accaatcatt tgaattttca tcacgttagt tgttccgcgt tcaccaactg gtacaccagc
      421 agtgatcaag attaaatcgc cttctgatgc gaaacctaat tcaacagctt ttttagctgc
      481 tagttcaaac atttcatcag ttgttgatgg tttttcagca actgttgggt aaacgcccca
      541 gtttaacatc aaaccacgtt ttgtgcgttc gtcaaaagta acagccaaga tatctgcatc
      601 tggacgatat tttgaaatca tttttgcagt gtatcctgat tcagttgcag caacgattgt
      661 tttcacgcct agattttttg cagcacgagc tacagataaa ccgattgttt cagttacatc
      721 tgttttatcg aattcgttca attggaatgt acctaattct gataatgcat tttcagcttc
      781 ttggtcgata cgtgccattg ttgaaacagc ttcaactggg tagtccccgt ttgcagattc
      841 accagatagc attgttgcat ctgtgccatc aaatacagcg ttagcaacgt cagaagcttc
      901 cgcacgtgtt ggacgtgggt tttcttgcat tgattctaac atttgtgtag cagtgataac
      961 tgggatacca gcagcattac attttttgat aatgcgtttt tgaaccattg gtactagttc
     1021 agctgggatt tcaacaccca tatctccgcg ggcaatcata acaccgtcag aaactttgat
     1081 gatttcgtca atgttgtcga taccttcttg agattcgatt ttagggaaga tttgaacatg
     1141 agtcatgtct ttttcttcta agatttcgcg gatatctaga acgtcttgtg ctttacgtac
     1201 aaaacttgca gcgataaagt cgatatcgtt gtctaaacca aaacgaatat cgtcagcatc
     1261 ttttggagta atacctggca aactaataga aacgcctggt gcatttaccc ctttacgtga
     1321 tcctagcata cctgcatttt taacttctac gactaattca cggtttgctt cgtctttttc
     1381 aatgatttgc atatcgatca aaccatcatc aaataaaatg tgtccgccaa cgtgaacatc
     1441 gtcaaataat cctgggtaag taaccgcgat tttttctttt gaacctacat gttcaggatc
     1501 catagagatg cgtgtttgat ctcctacttc aaatttgatg tatcctgcac gaccaaaatc
     1561 agcttctgtt gtgccttgaa cagttgtacg gatttcagct ccttttgtat ctaaaagaat
     1621 accgacatct tttcctgtct ttttaactgc ttcacggacc atagtcatac gtgataattg
     1681 ttcttcatgg tcaccatgag aaaaattaaa gcggaagaca tttgccccag attcaatcaa
     1741 ttgagaaata atttctactg agttacttgc tggtcctaat gtactaacga ttttggtttt
     1801 tttcatttaa aaaactctcc cattcaattt tgtttattta aagctgtaca cagcttaaat
     1861 agcttggttt ctttcactct taaaatgaaa gtgaattatt tagatcatat agtgataagt
     1921 ctggtttatg tttgttgttt tcaagtgtat caatgatgtc agacgcaacg atttcgttgt
     1981 catggatacc gacacataat ccaccttttc cttcttgaag caattcgaca gcgtaagcac
     2041 cgaatttact tgccaacacg cggtcgcgtg cgcttggtgc tccaccacgg acaacgtgtc
     2101 ccaagataga tacacgtgta tgatagtcac caaattctga aagtttctca gcgaattcat
     2161 ttccgcccat tacaccttca gccaagatga tcaagcaatg tttcttcccg cgatcgcggc
     2221 cttcttggat ttttttagca actgaagcca tatcaaaatc atgttctggg atgatgattt
     2281 catccgctcc gccagccaca cctgaccata aagcgatgtc tcctgcattg cgtcccatta
     2341 cttcgataac gaatgtacga acatgagaag tcgctgtgtc acgaatacgg tcaattgatt
     2401 ctagtactgt attgattgct gtatcaaaac cgattgtgaa atcagtccca ggaatatcat
     2461 tatcgattgt tccaggaata cctactgctg ggaaaccgcg ttttgtcaaa gccattgcgc
     2521 catgataaga gccgtctcct ccgataacca ctagaccttc gattccgaat tttttcaatt
     2581 gttcgattcc ttttaattga ccttcttcag ttgcaaattc tggataacgt gcagaataca
     2641 agaatgtacc accacgttgg atcttgtcac ctacatctgc cacatcaaga cgacgaatat
     2701 ctccagcaac taaaccggca aaaccataat tgatcccgta tacttccagt ccttcgtaaa
     2761 tacctttacg aacaacggca cgaacagctg cattcattcc aggtgcgtct ccgccactgg
     2821 tcaaaattcc gatgcgtttc ataataaaca ttcacctcat taaaatactt tatcctaagt
     2881 atgacttagg tttacaacca tttttgcgtt aaccacgact aacattctaa cattattttt
     2941 ctcacttgtc ttgatttacg gataaaaaaa tgaaaaaaaa tgaaagcaag tagtattttt
     3001 taccgtaagt tatttgaaaa ccacattatc ttcgccaagc agataaataa gttgcccctt
     3061 tatactcttg ctgtcatcca cccaataagc atctgacaga actacttttc gttgcgtttt
     3121 ttcgtgatac ataactactg gtatatatcc tgggtgtttc tgaaaaatca cttgtaattg
     3181 tttcattatc tcgggatctt cccgttcttc tgtaatacgc aaatagcaag tttgtgaagg
     3241 atactgggct tctaactggt taggatcagc aaagctttct ccaattagtt gcagttcctg
     3301 attatatttg ctgatttcgc tccgcccttg aacaaagatc actcgatcaa cggcaaacaa
     3361 attacgatat ttacgataaa gagttggaaa aactgtgaca gaaatttctc cactactgtc
     3421 ggttccttca acaaaggcca tcagttctcc tttttttgtc cgaatttctc taacttcctt
     3481 gatcatcact aataaattga ctggttgatt agggacgata tctgtaatca gctgaacttg
     3541 tttagctaaa cgaagacgat caaacccttc tgcaggatga cctgacaaat aaatacctaa
     3601 gtactcctct tccagcttca agcgttcttc tagtgtatag tcagcgactt cttcttcttt
     3661 taatgccatg attcccagca agtcaagact tccaccactg taaacgatat tttggatttt
     3721 tccttcgaga tcaagcatca attgtttcct gttaggagat aattcatcaa aaactcctac
     3781 tgctgtcagt gattgcagta actctaattt cagccaacgc ttatcgatcc gtatgaggaa
     3841 ttgatccact gactggaacg ggccattttg ttttctttcg tttaatatat cttctacaaa
     3901 atcacgccga atacctttta tcgctgtcag tccgaatctg atttcttcct tattgaccaa
     3961 ctcaaaactg gaattacttt gattaatgga cggcgaggaa agtttgagtt tatttttacg
     4021 tgcttctgca atatattctt tcaattttga tgtgttatgc cgaacagaat tcatcaggct
     4081 ggtaaagaaa gcacctggat gatgggcttt tagatacgcc atttggaatc ccacgaatga
     4141 ataagcaaat gcatgtgacc gattgaatcc atagtttgca aatttttcga tatattcata
     4201 tacggtcgct gctgtttttt ccgaatatcc ctgttgtaat gctcctgata caaaatgcga
     4261 cttttcttca tctaatacgt cttttttctt tttactcact gcccgtcgca gaatatctga
     4321 ctgccctaat gtaaatccag ccatagtcga ggcgatctgc atgatttgtt cctgatagac
     4381 gatcactcca tatgtgtttt tcaaaatcgg gatcagtgaa tcatctgggt agcgaatggc
     4441 ttcttttcct ttcttcctgg caatgaaggt atctatattt tgcatagggc ctggtcgata
     4501 caatgcattg actgcggcaa tatcttcgat gttttctgga tctaatcttc gtaaaacgtt
     4561 ccggatacca gcagattcaa attgaaatac tcctgaggtt tcgcctcgtt gaaacaagag
     4621 cagcgttttt gcatcattta gcgggatctc ttttaatcgg atctcatacc cttctactcg
     4681 cttaacagct ttcaaagtaa aatcaataat cgataagttg cgtagtccta aaaagtccat
     4741 tttcaaaaga ccgattgctt ctacctcatt catcgtatac tgggtcagaa aagcttcatc
     4801 agaaccgcgt tggagcggaa ctagttcaag tagattctta tcgctgataa ctacacctgc
     4861 tgcgtgagtc gaaacatgtc ttggcaatcc ttctaaaatc actgcagttt tgaataaaag
     4921 ttggtttttt tcgcttgcct gaactaattc tactaattga tgcgaagttt tataagcttg
     4981 ggtcaatgtg attttcaatt gattgggtat tgcttttgac cagcggtttg cttcactttg
     5041 cgataaacca aacactctag ctgcatctct aagaaccatt tttgctgcca ttgtgccaaa
     5101 tgttgcgatt tgtgccatat gatattgtcc atacttttct cgaacataat tcaatacttc
     5161 ttcacgtcga ttgtcaggaa tatccaaatc gatatctggc atcgtaaagc gttcggggtt
     5221 taaaaagcgt tcgaacagca aatcatattc taatggatcc acatctgtga tttctaacac
     5281 ataggctact aatgaacccg cagcagaacc acgacctgca ccggtaacga ttttgtttct
     5341 atgagcaaaa gccattacat cccatacgat caagaaataa tcatcgaatc ccatttgatg
     5401 gatgacagat aactcgtagt ccaaacgttc ttggtattcc tttgtatagt cagtgattcg
     5461 gcgttccact tgcgaatagc ataactctct taaatattct ccagcttgtt ttccattagg
     5521 aactggataa tgcggaagta atttctgatg gaactggaat tcaaatgaaa tcgattcagc
     5581 taaacgctgg gcgcctatta cagcttggga aagacccgct ttttcgaatc gtttgatcat
     5641 ttcttgttct gaaagaagat agacatttgc tgttagtggt aaagatgcag ataaattttc
     5701 taaaggtatt tggcttccct ctttgatatg tttcatcact tcaagtgcaa aaagttcttc
     5761 ttgttgtaaa ctagcgatgg gatgtaatgc acaaagcgaa taattttctt ttatcacata
     5821 ctgataccat tcaggcaatt gatcaggcgt gttttctgtc aagccgatat aaaaatttct
     5881 agtaaaaaca ttttcaagta aaaactgcca atgaccgaaa aagttatcct ctttattgaa
     5941 agcctcagaa acagaatgat ttgccggaac aacaaacgct agatggtcgg tttctgtcat
     6001 ttctgcaagt tccaattttt cttggatcat ttttcggctg gaaagtttca tcagctgctg
     6061 ataacctatt tcatcttttg cgtacgcaag caagccataa tttttttctt cataagtata
     6121 actaagatgt aatccaatga ttggctgtac tccttcttct acacaaatac gatagaattc
     6181 aggtgcaccg tataagacat ctttatccgt tatggctaat gaagtgtagc cttgtttttt
     6241 tgcttcttgt acatattctc gaatacgtat cgtactttgt aaaagagaat aagaagtaat
     6301 tgtataaagt tgtgggaaaa acatcttgca ttccgccttt tttttcgtta aaattcacta
     6361 gcgttaaaag gtttgatcct tttactttca ttaagatagc aggcgctgaa gttgattatt
     6421 ccacttcatt tgcttgcttg taatctgtag gaatgagttc tttcgcaaga actcaaattt
     6481 tattgagatt tggtctcgtt tctttacatt caaagaaaat ctggtaaaat taagatagaa
     6541 aagaggtgtt ctttatgcga tatatcgtta cattattctg ggctgtcgtt ctaggtcaag
     6601 tagtcggtta tattggtgct gcattaacaa gcggaacata tgattttact ttgacaacaa
     6661 tcatttcatt cattgcgggt gtcattatcc ttttgattgg tgcagttgcg ccaagaaaag
     6721 aaacctctgc ccattcgtaa aaaaacagcc aagagcttga gatttctcaa gctctttttt
     6781 attttctact cctcaccacc attataatct gctgcgaaga actttacgaa agttgaataa
     6841 agaaagaaga atatttgaca gaacaaacaa acttgtcaca ataaaaacag cactgtaatt
     6901 ataagcatgc gcgactgtcg atcctagcat gggacctaaa acttgtccaa agttgctaaa
     6961 catctgattg tagctataga ttcggctgac tccttcttga ggcgtaatct tactgatcag
     7021 tgtattgaca gaaggcatca aagcgccagt tgaaaagcct aaaataaagc gaagtatacc
     7081 taattggagc ggtgtagtga cgaatgccat aggtaaataa cagcaaaatg ataaaatgag
     7141 tcctccaatt agaacttttt gattcccaat cttatcccct agttttccta gtttcggtga
     7201 agaaaagaca gcggaaacac cagctatcga aacaatcaag ccgctgacaa aaaggatatt
     7261 ttctgtactt ccgctcaatt cacgaatata taaggtcaaa atagggctga tcgtagtgat
     7321 tcctatctgt aaaatcaagg tcgtaatgaa gagaccgatc aaaatagaga gatgatccat
     7381 ttttgaaaaa atttctttgg tgcttatcag atcttttttc tcgattggat ggaaatcttc
     7441 tttgactaag aaaatggtca ataccgttgt gatcatcaac aaagcccctg tgatcaagaa
     7501 tacattttcc ataccaaacc actgagccag tgcaccgccc attgaaggtc cgatcagatt
     7561 tccagcaatc gctccggtag acaatgttcc taacgcccat ccgctctttt ctctaggtgc
     7621 ctgtgaagca atcatcgctg tggcatttgg aatatatcca gacaaaatcc cattccaaaa
     7681 ccgcatgatc aaaagccaat acgcgtttgg tacaaaagcc aaagatccca ttgttaatgt
     7741 catccctgct gctgcacgga tcatcatgag ttttcggcct tttcgatcag ctagatttcc
     7801 ccagattggt gcaaccacgg cagatgcaaa agcagtcaca gagattgcta gtcccgaaaa
     7861 caattcgatt tgatcttttg gtgttcctag ttgctcaaca taaactggta tgaatggcat
     7921 aactaaacta atacttgcac cagtgaaaaa acagccaatc cacgcaatca ctaaattttt
     7981 tctccaatta atctccatct atcatccctc tttcgtctct tttatcatac ctattttcga
     8041 ataaacaagc aacgatctcg ccagtaaaaa gagaaaaatc ccgcaaaggt cattttatat
     8101 gtaacgttct ttttatgtaa aacaaaaaga gatcagaaca aatactgttc caatcccttc
     8161 tctttagtta aattgttgct agaatgatga agttgataat gaataaaata tcaacgatcc
     8221 acaaagcaac agaaacttga ttgtattttc ctttgattac tttcacaatt gtatagaaaa
     8281 taaatcctgc tgcaatccca taggaaatgc tatagcacaa tcccataaag atcgatgcaa
     8341 agaaggccgg taaagcttct tccatatcta gccatttgat gtctgcaaaa gaagccatca
     8401 tcattactcc aactaaaatc aatgctggcg cagttgcttg atttggtaca atggcgatca
     8461 gtggagagaa taaactactt aatgcgaata gcactgccac tactactgaa gtcaaaccag
     8521 tacgtccacc ggccccgatc ccagctgcag attcgacata agttgttgta ttcgatgtac
     8581 caaagatagc ccccacagaa gtagcgatcg catcggcaaa tagtgcttta tccatttttg
     8641 ttttgaaccc acgaccatct tccaacgcga tctcgtcttc ttttgagaaa atacctgtgc
     8701 ggcgtcctgt accgataaat gttccgatcg tatcgaaagt atcagataaa ctgaatgcga
     8761 tgatcgtcat caatacttga ggaatcttgg aagaatcact gaataaagat tgcatgcctt
     8821 cagaaccaaa tgctgcaccg aaagttgttc ctaattcttt aaatgaatta cctaacgaat
     8881 tcgtttgcca atcaatggaa cttaaatcaa caacacccat aaaaatacca aggactgtgg
     8941 tcgctacgat accgataaga atagccccac ggacattttt tacaactaaa atggctgtca
     9001 gtaccaaacc aatcacagcc aataagatag gagcgttgtc aaagttggct aacgcaggga
     9061 cgattccacc atttatcgta acatttgtcg cttttccgcc ttcaacgaca gagctcgaaa
     9121 tagctgactg gtctgctgaa aaagataaga atcctgcatt tttgattccg acatacgcaa
     9181 cgaaaatacc gatcccgcca ccaatcgcat gttgaagact ttcgggaatc gcccgaataa
     9241 tcatttttcg aatatttgtg acagtaataa aaatattgat taatccgcaa ataaacacca
     9301 ttgccaaggc ttgttgccaa gaataaccaa gcccaaaaac gaccgtaaaa gtgaaaaatg
     9361 cgttcaatcc cataccaggt gcctgagcat atggtacatt tgcgaacaat cccattacca
     9421 gcgtaccaat aatcgatgca atgattgtcg ctagaaatac tgcttggaag ggcatacctg
     9481 atgctgacaa aatcgtcgga tttacgaata agatataact catggcaaag aatgtcgtta
     9541 ctcccgccat tatttcagtt gatacatttg tattgttttt ctttaaaccg aaaaactttt
     9601 ccataaaaag ttccactccc tgaatttttt tctgatggaa aactataata ataaaaaata
     9661 agtaaaatag ggcaaatcca tcgaacaatt tcaaattata tagacgtttc attcgctttt
     9721 caacgttttt ttaacaaaaa aagcgaaaat aatacttaac gttcgtgttt agcttatgtt
     9781 tagctattta tatagccata actgtagttt ttgttaggaa tagctgattg ctcttgttaa
     9841 ttacctaaaa tatgctagac taaaacaaat gctagttgag gaaggaagga cccttattga
     9901 ataaaaaatt aattgccatc gatctggacg gaactaccct caatcaagat tcgatgatta
     9961 catctaaaac aaaagagact ctaaaaaaag caatcaatgc cggtcatcat gtcagtattg
    10021 ctacaggacg cccattccga atgagccatc agttttacca acaattagaa ttaacaacac
    10081 cgatggttaa ttttaacggc gcattagtac acatccctaa tcagcactgg gatggtgaaa
    10141 gagaaacgtt gatcaatcgt gaaatcgctt ttgaaatctt atcgcaaaaa aagcagctaa
    10201 atttagattt tatcgctgct gaaaatcgtg atactttttt tattgacagt tttgatttct
    10261 ttgatgaaaa aatttttgct tcttctcgtc caggagagaa gaatttgctt tctcctaaaa
    10321 atttgacgac aaatcctaca tctttgcttg tccgaacaga caaacgtttt gcaggagctg
    10381 tatctgctga attaacacgt caatttggca gttatgtcga tgttcggaca tggggtggac
    10441 ctactgccat cttagagatt gtttcaaaag gaatccaaaa agcaaaaggc gtgcaagaga
    10501 ttgctaacta cctgtctatc gatcaaaaag acgtcattgc ctttggtgac gagcataatg
    10561 atttggaatt gttggattat gcaggttggg gagtagctat ggctaacggt actgaccaac
    10621 tcaaaggcat tgcaaatgat gtcactcctt tgagcaatca ggaagacggt ttagctgttt
    10681 atctggaaaa actacttaaa ctataataga gaattagatt ccacaaaaaa gaggttgtga
    10741 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
    10801 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
    10861 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
    10921 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
    10981 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
    11041 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
    11101 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
    11161 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
    11221 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
    11281 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
    11341 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
    11401 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
    11461 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
    11521 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
    11581 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
    11641 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
    11701 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
    11761 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
    11821 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
    11881 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
    11941 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
    12001 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
    12061 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
    12121 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
    12181 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
    12241 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
    12301 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
    12361 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
    12421 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
    12481 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
    12541 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
    12601 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
    12661 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
    12721 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
    12781 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
    12841 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
    12901 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
    12961 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
    13021 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
    13081 ttgtacccaa aaacttctta ataaacaaga agtatttaat atttttttca taaaatcttt
    13141 catcatttcc tatccttccg cctatttaca ttcatgcttc attgtaccca aaaacttctt
    13201 aataaaaaag aaaaaatggg cggagcaaga aattctttac ctaatttcaa gataaaatca
    13261 actttttgtt tcacagcaat cacttaaagt tacatatcca actatttttt tcattaaaaa
    13321 ataaacttat ttttgaaaat tatgttttct taagtcgtga aacactttga tggatcattt
    13381 atttgtggaa aactacgttt tgttattcta aaaaacttat ataaaaaata gtaaggtttt
    13441 tatttcgaat ccataaaata ttcttggatt gcttctcgtg tttttttcac tttttcgcta
    13501 agcttttcgt ttttagccat atagaaataa aaaatgatat cgatcgtagc aaattgggcc
    13561 aaaagtgaat tagttgccgc acttcgattc gtgatttctt ttggtctaga tgtttgtacg
    13621 agtacatcgg ctatcttgct aagcgggtta tttccaaatt gcgtcaacgc aatgatttcg
    13681 atattcatgc ttttcgccag ctcggctaat gcgactacat ctgcacttct acctgagttt
    13741 gaaacaagcc aaagcacttt ctttttctca ttgctgacaa gttgcgggag caagacgtgg
    13801 atatcttttt caaaaatgat tggcttaccg acacgagacc atttttgcag aatatcttct
    13861 actactaaag aagaggcgcc aaccccatat aaaaaaacag tctctgccga ttccaaacac
    13921 tctacgactt tttggatcgt ttcttcttct agaatatcta ctgtttcttt tatcgctact
    13981 tgtgcattgt taccaagttt gtttttgata gagtaaaagg actcatttgc ttctatatca
    14041 ctaaaacctt catcgattcc agtctgtagt tctgttgaaa tcgtgatttt gaagtccgtc
    14101 agactttgaa aaccgatttt tttggaaaaa cgtacaactg tcggcgcact caacccggaa
    14161 gcttctgcta attcattagc tgtcattgta gggaccttct caatattacc gataatgtag
    14221 ttggccaact ttttttctgc tgaagaataa tcttccatca tactttcaat acgactaatt
    14281 acgctcataa gttcctccac ccttttaatg atagtctatg taagtatacc atatcagacc
    14341 cttattcacg gtcgtatact ggataaaaaa caaaaaaaca atccgcccaa cagaatatct
    14401 tatcccatca atcccacaga ttctattgag catctgtgtc tttgaggaat acctgttatc
    14461 tattgttcgg attgctttct atctactact tttttacagg aaatttacct attagttttc
    14521 tctgctgtaa tttttgactt ctgcttccat agcatcaata aagatattca aatcttctac
    14581 agctgtttct ttacttccat aacgacgtac gttcactgtt gcatcttcca attccttgtc
    14641 tccaacaact aattggtaag ggatcttttg tgtttgagag gcacgtatct tatagcccat
    14701 tttttcatta cgatcatcaa cttctacacg caaccccttc atttgcaaac gttctttgat
    14761 ttcataagca taatcgccat gtgcatcaac agaaacaggg atgattgttg cttgtatagg
    14821 tgccaaccat gttggaaaag cgcctttgta tacttctgtt aaataagcga caaaacgttc
    14881 cattgtagaa acaattccgc ggtggataac tactggacga tgcgtatttt ccccgtcttc
    14941 tccaacatat gtcaaatcaa agcgctctgg caataagaag tctaattgta tagttgataa
    15001 tgtttcttct gtaccaagag ctgttttgac ttgaacatct agttttggtc cgtaaaaggc
    15061 tgcctcgcct tctgcttcaa agtaatctag ttctaattca tccatagcgg ctttcagcat
    15121 tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat ttatctgtat tgtttggatc
    15181 gcgatagctc aagcggaaac gataatctgt aatattgaag tcagcatata ctgccaccat
    15241 caattcaagc gtacgtttaa attcatcttt gatttgatca ggacgaacaa atgtatgacc
    15301 atcattcaac gtcatttctc gtacacgttg taaacctgac aaagcacctg atttttcata
    15361 gcgatgcatc attcctaatt cagcgatacg aattggtaat tcacgataag aatggatcgt
    15421 gtttttgtag accatcatgt gatgagggca gttcattggg cgaagaacaa gcatttcgcc
    15481 atctcccata tccattggcg ggaacatgtc ttcttgataa tgatcccagt gtccagatgt
    15541 tttgtacaac tcaacatctc ccattattgg tgtatataca tgttggtaac ctagactaac
    15601 ttctttgtct acgatataac gttcgatcgt gcgtcggatt gttgcccctt ttggaagcca
    15661 gaatggcaaa ccagaaccta cttctgggga aaccatgaac aagtcaagtt cttttcctag
    15721 tttacgatgg tctctttctt ttgcttcttc acggagacgg atatattctt tcaaagcttt
    15781 cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt tgattgtttg agtttccacg
    15841 ccaataagca cctgccactg ataataattt aaatacttgt atacggccag tggatggaac
    15901 atgtggtcca cgacacaaat cgatgaattc gccttgttga taagcagtaa tcacttcttc
    15961 ttctggcaat tcactaatca gttctacttt gtacggatca cttgcaaaaa tttctaatgc
    16021 ttcctgtttt gaaagaacac ggcgttcgat cggcagattt tctttgacga ttttcatcat
    16081 ttctgcttcg attgccggca aatcttctgc agttacttgg ttttgtccat tatctgtatc
    16141 gtagtaaaac cctgaatcga tagctggtcc aacgccaaag tggatatttg gatagaggcg
    16201 acgcatggct tgggccatca aatgagctgc tgaatgacgg actaagccaa gtgcatcttc
    16261 atggtctggt gtgatgatct caatacttgc atcttcctcg attggacgag tcaaatcaac
    16321 gagttttccg ttcactttac cagctaaagc tttttttgct aagctttttg aaatgctttc
    16381 agcaattgcc aatgttgttg ttccagactc aaattctttg actgcaccat ctggaaaagt
    16441 aatttttatc ataatagttt cctccttgaa atgttttgat tgatcagatc atcaagctga
    16501 aaaacaaaaa aatcctagca tctcttgaaa agagatacta ggacgaaaag ttcgtggttc
    16561 cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt aacggtccga ccgcccttgc
    16621 ttcaacaagg gtttcgaaag tggtcatcct ttggttttct cctggaaaag tttcagccta
    16681 gcttttcctc tcttatcgaa gactctcaaa ttcagttggc tttctcatca atcaattatg
    16741 tgtttattta accactaata aatagaaaaa tcaagtggga aactaaactt attttttcca
    16801 ttccccattt acaccgtatg ttcctttttt catctcattg atgatgacat ggatgtgttc
    16861 ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata tctttcatca tattttctaa
    16921 ttgttccgga ctgcgacctt ctactaattc gacatgtacg aatggcataa tgctctctcc
    16981 tctttctgat cgttcttttt tcaactcgat tctatcatac tatgtttgtc ttgggtactg
    17041 aatcaggaaa aatggctgga tctttttttg ctctcaaggc actcaaaata gagaccgtat
    17101 ccacgacttc ttgcaaaaca gctccgaata atgcggggat tagtcccgta cttgcaatta
    17161 acatcaaaaa aatacaaata aagatgccaa tcagtacaga ctgtttcgct attctcatcg
    17221 tatctcttga aatcactact gcttttgcta cacgcaagag atcatctttc aagatgaccg
    17281 catctgcact ttcactagca gctgtagaac catgagcccc cattgcaata ccgatatctg
    17341 cagcagcaag tgctggtgcg tcattcacgc cgtctcctac catcgcaaca ggtctttgat
    17401 ctttagggat actttctaaa atggtcagtt tatcttgagg caagctacgg gcatgtacag
    17461 aatctaattg gactttttct gcaatcgttt gagcaatggc agatccatcc ccggtgatca
    17521 tcatcaaatt tttcacatca agttttctta gctccttgat tgtctttgct gcttctgggc
    17581 gaatctgatc cgaaaatagg atcgttcctg caaaagatcg atcaattgtt acatagattg
    17641 ctgtattgga cgtttctatt tcttcagctg aaacaaaaga ggcacgtcca atctttactt
    17701 catgattatt gactactgct tggattcctt gaccggtcac ttctttcagt tgactaacag
    17761 gatacagttg gctgtttttc acgttgttca ctaacgaacg agctaagata tggctagatt
    17821 cctgttctgc actagctgca tacagaagta gctcttcttt ttttattcca ttgactggct
    17881 gaatcgcctc tacttccaat atccctttgg taagcgttcc tgttttatca aaagcaatcg
    17941 tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt tacaacgatc ccgtttttac
    18001 ttgaacggct cattcctgca actaaagcaa tgggcgcagc tagaatcagt ggacaaggtg
    18061 atgccactac cagtacctca gcaaatcgta aagggtcttt tgagaaccac caagaaatac
    18121 caccgatcaa ataagcaatc aacgtgaaag ggactgcata acgatcagct aaacggacaa
    18181 atcgggcagg cttttctttt gattcttcga ctagtttaat aatcatttga tactggctgt
    18241 ctgcggcacg tttttcagcg atcatttgga tcgatccgtc tccatttaaa gaccctgaca
    18301 taatctgatc tccaggtttc ttggtttccg ggcgggactc acctgtcaaa gaagattcat
    18361 cgaccgaaga ttctcctttg gtcactcgac catctgcggg tacaagttcg ccaggcttga
    18421 ctagtaactg gtcatcaata tttatttcat cgattactat atcctgcaat tttccttctt
    18481 tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga ctgcagttct ttacccgctc
    18541 gtttattcgc gtaatcctct agactatctc cgcccgtaag catgatcaat atcatcaaac
    18601 tagcccagta ttctttgacg gctaatgttg caaggattgc ggtaatcgct aatacatcta
    18661 cgccatatct tcctgaacgt aacgtcttga tcatttccaa aaacatcaag aaggcgagaa
    18721 tccctccagt aaatgcgaca atcgcaaatg caagggccgg ctggtgaaaa acaaattcac
    18781 ttaataaagc tatcccacca ataagcaaag taagtaaaaa ttttttgaaa tgtgtcattg
    18841 ttttttctcc tcttttatgt gacttagaga ggttagtaat aaatttccag ctgcctcttt
    18901 catcttaagc atagtccttg actagcactt tttaaaacaa taggccttaa ttgaaaacaa
    18961 ctatcactat cagcgtttcc aaaattcatc agttctacaa acaaaaatat tcagcaagga
    19021 ttactttttc gattcatcag ttaaaaagta ccttttttcg aaaaactatg ctatactttt
    19081 aattcgttat ttacggcgga aggagcaaac atgcgatgaa tgagaaacaa ctagaacaaa
    19141 cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca aaatatgctg gatacaaaac
    19201 aacaagaatt gactcaggaa atgaatcatt cgacaaaaga cacagctaat cacaagattc
    19261 gagctggttc caatgaagct ttttatgaat ctgctgttga atatcgccaa catgagcagg
    19321 aattgctttt gaaataccat actcttgaag cacagcaaaa acgcctgcaa acgttgtctg
    19381 tcatgaaaga tagtccttat tttgcccgta tcgactttaa agaagaccgt gaaacagaaa
    19441 cattatatct aggtatcgct tctttaagag atctggcaga agaaacaatc gttattgact
    19501 ggcgcgcacc aattgccaat ctttattatg aaggtgagat tggtcccgca ttttatgaaa
    19561 ctgaaacaga tagaatagat gtcgatctgc agttgaaacg acaatttaaa atccaagaag
    19621 gacgtatttt atctatggta gatacgtcgg aagtaatcaa tgatgacttt ttgctagaga
    19681 tattggatga atcatcaagc aaccatatga aaaacatcgt ctctacgata caaaaagcac
    19741 aaaatgcaat cattcgggac acaacaagta aagtaatgct gattgaagga atcgccggaa
    19801 gtggaaagac atccgctttg cttcaacgta ttgcttttct gttgtatcat aatcgcaagt
    19861 ggctcgaggc tgaaaacgtt cttctatttt cacccaacca tttgttttct gattacatct
    19921 cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac acagacattc aaaaattatt
    19981 tggtgcagct gctgccagag ttttctttac tagatgaaca gcaacaagaa agtggttttt
    20041 tgctagggga aaaagatccg atacaagtga tgaaaagcgg attgacctta gtcgatcaga
    20101 ttgatcgcta tatccaaggc atcacaaact acggtccctt gttccgtgat atgaaaataa
    20161 atggccgtac gatcctatca aaggaatcga ttcgccaatg gtataaagaa acaaatgaac
    20221 aattaccttt acatcataga ctttctctat tacaaacgaa gctcctgaaa aaattaggcg
    20281 gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga tctggcggaa gaacagctcc
    20341 aagaactata cgcaacagat ccaaatcttg aatatacaga acaaaaggag cgttcattac
    20401 gtaaaaaact ggccaaccaa atcgtacgta aaagattccg tcgaatccat cgagggatca
    20461 tgaattatca atttgtcaat ataacgaaac aatatctcca ctttctacaa actgtgccac
    20521 tttctcttct aacacgacat aaaatcagcg agaccaggtg gcaggaacat ttgacaactg
    20581 ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga tgctgtacta ttctttttac
    20641 tgatgcgccg aacatatcca gtgacggtta cacacaaagc tcgctatatc tttattgatg
    20701 aaatgcagga tttcgcacca tcacaagtgg cattgctgca agccttgttc ccaaaagcaa
    20761 atttgacttt ctgtggagat ctaaatcaaa atgtctttgg aaatgaaacg atcgttggat
    20821 cattggaaga ccttttcgtg gaccgtgaag tcactcgttt ccaattaacg acaagctatc
    20881 gctccacaaa agaaatcact gattttgcta atcattttat tgtgaaagaa catcaagtag
    20941 aaacaacagc aagaaaagga gaccttcctt tgctcatcca aggaacaaca gatgatatca
    21001 aattgaattg gttagcagat accattcgaa cgactcaggg aaaagcccgg tattggcgta
    21061 cagcaatcat agctaaaaca caagaagaat gcgaagctct ttatgctcaa ctgccagacg
    21121 agttgaaaca acaagtccag ctgattttaa atgagagtga ctttatgaag cgttccatta
    21181 ttttacttcc agccttttta gcaaaaggtc tggagtttga ccgagttttc ttgtgggatg
    21241 tagatgcaga acatttttct tctcagcaag acaaattgat cctttacacc atgtgtacac
    21301 gtgcgatgca tgaattgacc ctttttacct ccaataatga aagtcctttg ttaaacaata
    21361 tggatatttc atcttatgag aaaaaagtac tgtaagtaaa aagacaactg ctatcattaa
    21421 tgttagcagt tgtcttttta tttacaataa taattgcaga aaaagtaatt caatcattta
    21481 aggtcattta ttctatatat aaatccaaat ctgtttcagg atattttaat ttgacaagcg
    21541 aaataatcat tttcagttct tccatgtttt ctggatattc tgggtcaaat accttgctat
    21601 gacggccaat cccgccattg tcgatcaatc ggtaaattcg attccattca atgaattggt
    21661 caattgattg gaaattgctg attggatagc cgccacaatc caaaatctct gatacacgaa
    21721 atgctaggta tgcactatcg atcactttat tttttttcgt gtctatcttc tcatttcttt
    21781 cgataagaat tttttcagga cgatgtttca agctccactc taatagtaga aagaaagcct
    21841 ccatccgata ctcttcttca aataaccgaa aggctgaatc agaacgacga aaaagcagcg
    21901 gaatcagata attttgtcta taaatcagca ttggtgcgcg gtccaccata cactctaaat
    21961 aacggttcag ataccctgta ccgacatagt ttcctaatcg atagccttca acgccgaatc
    22021 ttgcgtattt ttgcgctaat tttgaacaat cagttacgtt cacagctaac tcttctactt
    22081 tgcggtaacc gcttaagata cgttcaaaag gttctaaagt gatttttgct tccatattct
    22141 tccccgtcca tcttccacat acttcatatc atttcacatg atacttatgc tctcatcata
    22201 ccacaggttg aattagaaaa gaaaataaat cacacaaaaa agcaagacaa tctgttagtg
    22261 agattgtctt gcttttcgaa aaagactgaa tacttttcgt taaaactggt tgcttagtct
    22321 tctaatgcca cattatggaa cacatgctgt acatcttcta gatcttccag tgcatcaatc
    22381 attttttcaa atgtttcttg atcttctgga gatagtgtta cctcgttttg agggatcatt
    22441 tggatctcag cgattgaaaa atcagtgatt cctttttctt ttaatgcttc ttgtatacta
    22501 tggaaatccg ctacttcacc gtaaacgatg atttgtcctt cttcttccat tacatcgcga
    22561 acatcgatgt ctttttccat taggtattca aggatctcgt cagcgtcttc tcctgcaaaa
    22621 gcaaagattg ctgtattgtc aaacatgtaa gaaaccgctc cggaaacacc catgttcccg
    22681 ccatttttcc caaatgctgc ccgtacgtct gctgctgttc gattcacatt gttcgtcaat
    22741 gtatcaacaa tcaccattga gccgcttggt ccaaatcctt catagcgcaa ttcagagtat
    22801 gtttcatctc ctgcaccttt tgctttttcg attgctcggt caataatatg ttttggtaca
    22861 ttataagttt tagcgcgatc aatgacgaaa cgtagttttt ggtttgcatg cggatcagga
    22921 tcacctgatt ttgctgccgc gtagatttca atcccaaatt ttgcataaat ccggctatta
    22981 ttagcatctt tagcagtttt ctttgctacg atatttgccc atttacgtcc catattttca
    23041 cttcgctttc tgatttgata gtttcataac gcaaaaacgt cctgttccat tatacttgtg
    23101 aatcacgatt ctgtgaagct ttttttcttt cttccttcat tcttctcatt tttagtcaat
    23161 tatctatact cggattggga tttgcccgaa attctgttcc ggaatattgc ctcccaattg
    23221 atagatttga tcacttctaa ctaacagttc cgcttcttgt gcctcttttt ttccgattcg
    23281 ggaataaata gaaatctctt ttttttcttt taagaatctt cttccagcat cggtaaatcc
    23341 aagtagtcga agataatttt tattttgttg gacaggaatc tcttgctttt tgatattcag
    23401 caagacataa caagataatc gttggatccg tgtccatgta tatctttttg tttttacctt
    23461 tgacacaaaa tcctcaaaac tatcagcagt tgcagctgct gttttcatgc gcgattctat
    23521 tccttcggtc atctgataga tatctgtcag ttccagcaaa gtagaagata acaaccgata
    23581 acgcaaatac ggccaataag attcccaagt ctgcagcgaa caagttgaaa gatcttcttc
    23641 tgtctgaggc ggcacaagtc cagctattct ttttccttct tctaacgcat ggcgaatggc
    23701 tgttgcactg gctatcctct gatctgtgat gattttttca tgatagcctg cacttttacg
    23761 tcggatcgga tgtaattcca tagggtggac atacttggca ttttcctttg cataacttaa
    23821 tcccaagata tgatttggcg aagaaaaatc aagcgtaata tcgggataca gactgcgaaa
    23881 aacctcagtc attttttgcg gataactata cgtttgatca gacagttctt gaaatgcctc
    23941 ttctatccta tcttgattct cttggacaaa gcgtccgaaa ctgctgtaat caaacgaagt
    24001 ctcttcttct gttccaaaac acaaactgtc acagtccagt gtttgcaaga tttttactgc
    24061 acctttagca aaatagtcag ctgactgaac cgcccattcg acgggaagct ccactactaa
    24121 atccacacca ttttgtaaag cttcattcgc gcgatgccat ttatcgatga tagccggttc
    24181 gcctctttgc aaaaaattcc cgctcattac agcaatgacg acctctgctc ctgttatttt
    24241 tcgtgccatt tccacatgat agcgatgtcc attgtgaaaa ggattgtact ctacgataat
    24301 cccgcagctt ttcatgcgca tcctccttca tagttatcga taaaaaagaa atagagacaa
    24361 gtaaaaaaag gcaaaaatcg gaaaaaatac ccgaactata taaactattc tcgcaaacac
    24421 aatacttctg cgacgaaaag ctgcactgca agagcaatgc ctatatcgtt cggatatata
    24481 atttatgttg aagatttatt cttttcttgt taactcgagc aggcaaaacc gctagtcttt
    24541 gtatgctaca aagaaccagc gctttgattt ctctgatgct gcttcgtctg taaaatcaga
    24601 aaatgcttcg actttcgaaa agccgctgtt ttccagcatt cttaaataac tttccaatgg
    24661 ataggtacgc tcttgatgta gttcatcttc acgaataaat cgaccagacc cttcttgttc
    24721 ttcaacgaaa aaggtaagaa aatgttcgat gctgtgtttc tcgcttcctg gataactatc
    24781 ccacaaaaaa gcaaaattat ccccttgata atgatagcta tattctggaa aaacctcatc
    24841 tacttgatag gtggagtgta cgtcaaataa aaatcggcca ttttcttcta acgcttgata
    24901 gacttcatca aacacttgct gtacctcttg tctatttttc atatagcaca gcgaatcaga
    24961 aaaacaggta attgcctgat attgtccgat ttctgataaa tcgagcatat tgccttccac
    25021 aaattggatc gatgcgtctt cttcatatgc tcgctggctg gcaatcatca acatctcttc
    25081 tgaaaggtct aaccctgtca ctttatatcc atcttttgca aaggcaaccg ctaatgctcc
    25141 agtaccgcat gccagttcca aaatattttt tgtgtcttct tgtaaatgac gtttcgaaaa
    25201 agctaaccat tgctgataaa gtgtttcgtc catcacttca tcgtaaatga aagcaaatgt
    25261 ttcataagcc atatgttaat ccaaccattc actaagatcg actagcggag catctgacca
    25321 tagtttttcc agattataga aatttcgttc tggcgcatgg aaaacatgta cgatcacatc
    25381 tccaagatcg atcaataccc attttccgcc ttcttttcct tcaatacgtt ttacttcata
    25441 tttgttttct tcttctttat caatgatttc ttctgtaatt gcgttgattt gacgttcgct
    25501 atttgctgaa cagatcatga aataatctgc caacaaggaa acatttcgaa cgtctaaagc
    25561 gataatgtct tctgcccgtt ttgaatctgc tgcttcaatc gcaattttta attgttcttt
    25621 actatctatg atgatttcct cctaatatga gcagcttatt tttttgctac ccagtaatta
    25681 tatgtttcga ttgtttttgg ataaatctgt tgttcttgcg caagcaaatg ggccaatgta
    25741 tgttttgttt caaatgctac agcttcgtct agatcagccc atgcaatggc tcgtgcatct
    25801 tgtaccccag gaaaatctcg acctggttca atatagtctg ctacataaat gattttgtca
    25861 aggagactca tcttagcagc cccagtcgta tgcacccgga ttgcatgaag aatctctgca
    25921 tcggtgattc ctaattcccg ctctacaaac gaagcgccaa caagaccatg ccagattgca
    25981 ttattataat tcaataactc cggatcaaaa ccgtctctta cgattaccat tttgaactcc
    26041 tcatctggtc gttcttttgc ataatcatgt gtcaatgcag caatactagc tttttcagga
    26101 gaagcaccgt attttttggc aagagccacg gctgtttctt ctactccaag cacatgcttg
    26161 aagcgttgtt cgctcattct catctgtatt ttttgcatca ggttttctct agaataagca
    26221 gtatattcat tactatagtc catattcata caacccttca ttttgaatat aatcaattac
    26281 tttatctgga acaaggtagc ggatagaaca gccttctttg atcttttgac gtattttcgt
    26341 ggaactgatg tcgatctcag gtacatctac ccaaattaca ggatatggag tatccgttgt
    26401 gtatcctggt cgacgtatac ccacaaagtt gaccatagaa gtcagctcat cgattttata
    26461 ccattttggc aaatattcta ccatatcccc accgataata aagtaataat ctgtatccgg
    26521 attgttttgc gtcaattcct tcatagtatc ataggtgtaa cttttcccac cacgagctaa
    26581 ttcaatcgtt tcaatctgca aaaaaggatt gtcttccacc gctaattcca acatgttgag
    26641 tcgatgtttt gcatcaatcg tctgtttctc atcaacatga ggtggctgat aagaaggcat
    26701 caaaaatacg cggtcaagac ctaaattccg gccagcttgt tcagccatca ctaaatgtgc
    26761 aaggtgaacg ggattgaatg ttccgcccaa gatccctact tgctttttct tctctaagaa
    26821 ttgaggcatt tcttcaggaa ataattgtga acgaacaaat gtcttagctt gcgtattcat
    26881 catggtcact ccttatattg cttttacttc ttttgatagc ctttgatact tttcttttgt
    26941 ggaaggtttg tataatacaa taacacgtcc aatgatttgg acgatttcgc aatctatttc
    27001 tttttctaat acttgagctg cttcttctgc aatctcatct gtattttgaa gtaacgatac
    27061 tttgatcagt tctctttttt ctaaagcttc ttcaatttga acgatcattg ctgaattgat
    27121 cccacctttt ccgatttgaa aaatcggctg aagatgatgc gctttgctac gtaaaaaacg
    27181 cttttgtttt cctcttaaac tcataatttt ccttcttcct taaatcaatg ctttgcgttt
    27241 caatacatct acaccctttg gcgcccagcc cgcaattact actggcgctg ttacagtgat
    27301 ccagcctaga ccggcaaaaa cgatgtctgt tttttctttc acagaaaact caaaacgaac
    27361 aagctccgga aatgcctcga cttcatcagg acgaggaggc tgtaataatc cacctacatg
    27421 tttttgataa aattcgtctg ctttttccag tttcgttcga tgaatattta catcatttga
    27481 cacataagca ataaatgaag cacgattgcc gctgataaaa tcaaatcttg caagtccgcc
    27541 caagaaaagt gtttgccctt cattcagctg ataaacttta ggtttgattt cttttaacgg
    27601 tgcaatgatc cgcaaatctt ttttacctaa gtagtgcgcc atttgatggc gatggataat
    27661 tcccggtgta tcaatcaaaa aatgcccgtc atctaaagga atctcgattt tatccaaggt
    27721 cgttcctgga aactgcgaag ttgtgattac atcttgtact cccgctgtat ttttgatgat
    27781 ttgattgata agcgtcgatt ttccgacatt tgttactccg acgatataga catcccgtcc
    27841 atcgcggtat tcttcgattt tttctaatag ttccgtcatc tcatgagatt tcttagcact
    27901 agttaataat acatctactg gacgaattcc tgcttcgtaa gcacgttcct tcatccattg
    27961 tgttaattta gtttttttca atgatttagg taatatatcc actttgttcc caactaaaag
    28021 tactggattg tcaccgacaa aacggtgtag tcctgggatt agtgaaccat tgaagtcaaa
    28081 aatatccacc acattgacga tcaatgcatc tttttgccct aattcattca gtaagcgcaa
    28141 aaagtcgtca tccgtcaatg agacatcttg ggtctcatta taatggcgta atctgaaaca
    28201 acgctgacaa tagacttccc cggaagctaa acctttttcc aaagccgttt gcggcgtgta
    28261 tcctaattca ccgggtttgt ttgtttgtat aacggcacca cagccgatac attgtaattc
    28321 ttctgtcatt cgattccacc tcgccagccc atatcagggt gttttgctaa tagataggcc
    28381 ataattcttt tttcccaaaa acgattaaat tgtgtcttcc aactatctgt tgcgataatg
    28441 ggttttacaa ggatgctacg tatccctgca cgattggctc cacgtatatc agtcataatc
    28501 tgatcgccta ccattactat ctcatcatct gctaaatcca attgtttctt tgctatgttg
    28561 atccctcttg ctaaaggttt cattgctcgt gcaacataca aaagatcaaa tttttcaatc
    28621 gctcgcgcga cacgttttga attattgttt gaaacaacaa caacagggat tcctgcattt
    28681 ttcatttcta aaatccaagt aagcaactct tctgttccat ctggattatt ccatgcaatc
    28741 aacgtattgt ctaaatcggt caatactgct ttgatgccta gattctttaa ttgggcaggt
    28801 gtaatctgat agatggcttc taccatccat gttggtttgt aatttgaaaa catgctgtct
    28861 cctcatctgc aaaaaatgaa ggagcacata aagtactcct caaccccgta ttatactgca
    28921 tatcagcaaa aaaaactacc tctatactcg ttcgtctttc agaaaaagca tttctttaca
    28981 aaatgaccaa aaataaagat tgatcacaga aaaatttaga aacaaaaaac aactaatatt
    29041 ggcggaacct cttgtatcgt ttttccagta atttatgctg atctaatcga acaagttccg
    29101 tcaatctttc aatcaacgct tttttcataa tgtgtgcgac tttatatcgt ggcaactgct
    29161 cgccgttgaa cgtttcagga ataactcgat caacgattcc taactcgcgt agttctggcg
    29221 cagtaatttt catcaactca gctgcttctg cggcgcgact accgtctttc cacaaaatcg
    29281 acgcaaatcc ttctggagaa agcacagcat aaatcgtttc ttccagcatc cagacttcat
    29341 ccgctaaacc tagagcaagg gcaccgccac ttccaccttc accaatgatg atggcgatga
    29401 tcggtacctt taaatcagac atttcgagca gatttctggc aatagcctct ccttcgccgc
    29461 gctcttctgc tcctacaccg caatatgcac ctgctgtatt gatcaaggta atcaccggtc
    29521 tattgaactt ttccgcttgt ttcatcagtc gtaaagcttt tcgatagcct tcaggatggg
    29581 gagaaccaaa atttctgcgg atattttcct gcagatcccg tcctttttga ataccgatca
    29641 ctgttaccgg tttgccatct aatcgagcga ttccaccaac aatcgcttcg tcatctgcaa
    29701 ataaacgatc accatggcat tcctcgaaag aatcgaagat ctcttcaaaa aaatccaagc
    29761 ttgtccatcg ttctttagct cttgctaact gaacgacatc atgtgctgtc ttatccacga
    29821 tctatccatc ctttctgtgt atgcaattca agaagcgtat ggattttctg tttcaattcc
    29881 atgcggggaa caatctgatc gacaaaacca tgagataata agaattcagc cttttggaaa
    29941 tcttctggaa gttcctgctt gatcgtctgt tcgatgacgc gtcttccagc aaaaccgatc
    30001 aagctttggg gttctgctag aataatatcg ccttccattg caaaactggc agttactcct
    30061 ccggtcgtcg ggtccgttaa aacagttaga taaaaaagac cttcattgct atgccgtttg
    30121 acagcagcag agatttttgc catctgcatt agagaaaaga ttccttcttg catccgtgcg
    30181 ccacctgaag ctgtgaacaa gacgactgga agactttcct ttgttgctcg ttcgaataat
    30241 ctggtgatct tttctccgac aatcgttccc atacttccca tgataaaatt aggatccatc
    30301 actccaataa tgaagggaat cccctgaatg gttgctttac cagtgaggac tgcttcatga
    30361 agtcctgttt tatcttgcat ttttttaatt ttttccgtat attcgggaaa tgctagcgga
    30421 tctttggtta caagatccgt atcccattct tcaaaacttt tttcatcaat aatcaaagct
    30481 agacgctgcc atgcaccgat tcgaaaagcg tatccgcaat gagggcatac tttttcttca
    30541 ccgatgtctt tcgtataaag gatatgtttg caatttggac actttgccca catgttatca
    30601 ggaacagatg gcgagttagc aggtttgctc tctctattag gattgatccg aatatatttc
    30661 tttttcttaa acagagccat ctatgcgcct cctatctccg tttctggtgt ccaattcggt
    30721 aaaaattctt cttgtaaaaa tgctgtgctg taatcacctg ctatgactgc aggatgacta
    30781 atcaaatcca tctgaaattc tgcgttcgtg atgattcctt ctgtgataaa ttcacttaag
    30841 gctcgctgca tcttcattaa tgcttcaaaa cgcgtatttc catggacgat tatttttgca
    30901 atcattgaat cataatatgg cgggatcgta tatcccgggt aaactgcact gtctactcgc
    30961 agtcccatcc cacctgctgg aagcagtaag ttttggatct tccctggaga aggagcaaaa
    31021 tggaaagcag gattttctgc attaatccgg cattcgattg cgtgtccttg gaatactact
    31081 tcttcttgac acacattcaa aggctcaccg gctgctatct ccacctgttt ttttactaaa
    31141 tcgattcctg tgaccatttc tgttactgga tgttccactt ggattcgagt attcatttcc
    31201 atgaaataaa aatcgccttc ttcatccatc agaaactcga tcgttccggc attttcataa
    31261 ttgactgctt tagctgctcg aacagcagcg tctcctagtg cttctctttt tgtttgtgag
    31321 atcactacag aaggagattc ttccagtact ttttgattgt tgcgttgtaa agaacaatct
    31381 cgctcgccta aatgaatgac atgaccaaat cgatcaccta agatctgaac ttctatatgg
    31441 cgagcaggat aaatgatttt ttccaaatac atatcatcat ttccaaatgc cgctgctgct
    31501 tcttgctgag ctgaagaaaa atgctgtggg agctcttctt ttgattgaac tttccgaatg
    31561 cctttcccgc caccacctgc agctgctttc agcatcactg gataaccaat tctgtctgct
    31621 atttccaatg cttcttctac tgtagataat acaccagtac ttcctggaat gactgggaca
    31681 tttgcttctt gcatcaattg acgcgcattg atcttatttc ccattgcatc gatcgttgca
    31741 cttttaggac caataaatgt gatattgcac tcttcacaca tggaggcaaa tcggctattt
    31801 tcagataaaa aaccaaatcc tggatgaatt gcttcggctt ttgtcacgat tgcagcgctc
    31861 agtacttcct gaacattcag atatgaatca acggcttttg ccgggccaat gcagattgct
    31921 tcatctgcta actgtgtatg aagtgcttcg gcatctgctt ctgaatatat cgctacggtt
    31981 tggatcccta gttcgcggca tgcacgaatg atacgtacag cgatttcacc gcggttagct
    32041 atcaatactt tagaaaacat atttcaccta tccaatcata aaggttaatt ccgcttcggc
    32101 tactttttta ccgtctactt tagcgattcc tttgcctatt ccggctgaag ctttcacttt
    32161 caatatctca acttcaaggt ataaggtatc tcccggtgta actttcttac ggaattttgc
    32221 tttgtccaat ccgccgaaat aagccgtctt tcccttgaat tgttcaagag aaagtaatgc
    32281 aactgctcct gcttgagcca ttgcttcaac aatcaatacc cctggcataa caggttcttc
    32341 tggaaaatgt ccttgaaaaa agggctcatt gatcgttaca tttttcttag caacgatccg
    32401 ttctccttca accatttctt ccactcgatc aatcaaaagc attggataac gatgtggaat
    32461 gatttctttt atttcttgta tgttcattgt tttccctctt tcctgctttg cttatttttg
    32521 ctgaatccta aagagtggct gtccatattc gacaactgac tcgttttcta caaggatttc
    32581 tgtgatcact ccgtcggcct cactagttat ctcattcatc agtttcatcg cttcaataat
    32641 gcagataact tctcctttgg caacttgatc acctactttt ttgaaagctg gtttgtcggg
    32701 cgctggttgc agataaacga ttcccacgat tggcgaaaca acctcgattc catcaaatac
    32761 tgccgattct ttttctttca ttatttctgg agcagttgtt gcttgttcag gtatgacatt
    32821 gactggctcc gaaactggca acgtttcgtt tgtttgaacc atattcatgc ctcgacttgt
    32881 tttattttta ttcatataca attcaaattg accttcacgc aaatcgaatt ccgttagcga
    32941 ggattgatca aattgactga ctaattctct gatttcattg atatccaaaa ttattcctcc
    33001 cagcgtttca tgcacagaac cgcattatgt ccgccaaacc ctaatgaatt actcattgca
    33061 taagtaaaac ttgcaggttt gctttcgttt ttgacaactg tcaagtcaat ctcaggatct
    33121 tgtttttcta cattgatcgt aggtgggata aattgatgtt ctaatgccag caaagttgcc
    33181 aaggcttcga tcccgccggc tgctcccaat aaatgtccag tcatcgattt cgtgctgctg
    33241 acgtaagtat ctctgaaatt ttcacctaat gcgatttgga ttgcttttgc ttctgcctgg
    33301 tcattggctg gtgtacttgt tccgtgagca ttgatatatc cgacttgttc cggctgtaca
    33361 ccagcttctt ccatcgcaag cttcattgct ttagatgcac cagaaccatc tggagtagga
    33421 gctgtcatat ggtaagcgtc acagtttgca ccgtatccaa cgatttctcc atagattttg
    33481 gcaccgcgag cttgtgcatg ttctaaagat tcaagaacta gaacgcctgc tccttcaccc
    33541 atcacaaaac cattccgatt ttcgtcaaac ggaatcgaac cacgattagg atcagttgat
    33601 tctgagagtg ctgttaaagc tgcaaatcca gcaatcccca tctcacaaat cgttgcttct
    33661 gttcctccag caagaatgac atcagaatag ccatgtttga tgttacggaa tgcttcccca
    33721 attgaattcg ttccgctagc acaagctgtt acgatagctg tacaaatacc ttttgcacct
    33781 acacgcaaag caatatttcc agctgccatg ttggcaatcg ccattggtac gaacattgga
    33841 gaaactcgtt ttgcaccttt ttcatgtaga cgaataactt ggttttcgat tgttggaaga
    33901 ccaccgatcc cagatccgat gatgacgcca aaccggtctt gattcatttt ttctttatct
    33961 aaaccgctca tctccattgc ttctaaagca gcgtagattc catagatcga aaacatatcc
    34021 attcgtttgc tatctttttt aataaaatat ttatcgtaag ggaaatcttt gacttcaccc
    34081 gctaaggtaa tccctgtttc tgaagcatca aatttagtga tcggtgcgat gccattcgtc
    34141 ccctctttta agctattcaa aaagctgtct gcgtcatttc caataggtga agtgactcca
    34201 tagccagtaa ttaccactcg attcatttgt ttgctctcct tttcttatcc gtgcatgacc
    34261 aatccgccat cgacattgat gacttgtccg gtgatatatg gattttgtgc taagaaaact
    34321 gctgtttgtg caatatcttc tacttgtccg aaacgctgca tcggaatttg cttttccgct
    34381 tgttccttga ctttgtctgc taaaacttcc gtcatatcag ttgtgatgaa tcctggcgca
    34441 atggcattac atgtaatgcc tctagttgca gcttctcgtg ccacagattt tgttaatcct
    34501 actacgccag ctttactagc tgcataattt gcttgaccaa tatttccaat caacccagat
    34561 acactagaga gattaatgat ggccccttct cgctgcttca tcattttttt caatacgtgc
    34621 tgcgtcatat tgaaggttcc aatcaaattg atatccagac attttttgaa atcttctgca
    34681 tccattcgca tcacaagttt gtcatttgtg attccggcat tgtttactaa cacatggatc
    34741 gatccaagct tttcttctgc ttctttgatc atttgtcccg ctttctcata atcagagatg
    34801 tcaccggaaa ctcctacgca ttttacaccg aatgcttcga tttcttctat cttttctttt
    34861 ggaatctctc cgcgaccatt caaaatgata tttgctccag cttttgcaaa agctaaggcc
    34921 atcgctttgc cgattcctct tgtactacct gtgataaata cattttttcc ttttacatcc
    34981 ataacgttcc tccttatagt attgctattg tttcatcaaa tgtctgctta ttctcaacac
    35041 gcagaactgg tattgtttta tcgatttttt tcataaatcc gcttaatact tttccggggc
    35101 cgacttcgat gacttgttcg atgccgattg tttttaactt atggatactc tcatagaaac
    35161 gaacaggttg catgacttgc tgttcaagta gtcccgcgat tgtttctttc ttcattattt
    35221 ccgttgttgt gttgcttatg ataggaaaag aaggttcaga gaattgaatc tgacttaggt
    35281 ccttcgccag tttttttgct gccggttcta aaatagctgt atgaaaaggc ccgctgacat
    35341 ttagagggat cattcttttg aacccttttt cttttaatag taaaacagct tcgtctactg
    35401 ctttttcttc gccaccaatc acgatctgct gaggtgtatt ataatttgca ggagatacga
    35461 tgccgtactt gctagcttca tgacagcttt cctcgatcgt ttcaatcggc gcattcatca
    35521 ctgcaaccat tttcccgctt ccagctgggg cagcttctgt catataagcg cctctttttg
    35581 ctactaacgc aactgcttca gtgaaggata gcgcattact agctactaat gccgaatatt
    35641 ctcctaaact cagtcctaaa gctgcatctg ggatgattcc atgctccttc aataagcgat
    35701 aatacgcgat gctgactgtt agaatagctg gctgtgtata ttgcgtctga tccaaacgtt
    35761 cattttctgt aaagcaaagt tcagccatat cgtagccgag aatctcactt gcttcatcaa
    35821 atgtctgctt tacaacggct tcttcatata aatcttttcc cataccttga tattgtgcac
    35881 cttggccact aaataagaat gctgttttca tgtttttctc caatatatcc agaatatatg
    35941 tttgattctt cctattcaaa aaggccgaga cattcctatt tcaggaaatg tcccagcccc
    36001 caacctaatt ttaatgatta tgcagtttgt ttttctacgt aagtcactaa atcttgaacc
    36061 gttttgattc cgtcttctgt ttcgattttc acgtcaaatt catcttcaat ttcattgatg
    36121 atttggaata aatccaagct gtcagcttct aagtcttctt ggatgtcagt agtcaatttg
    36181 atttcttcag tttcttttcc taattcttct gcgatgattt cttgaatttt ttcgaatacc
    36241 ataataatat attcctccga tattgtttgc tttttatttt tataatttaa cagtctacaa
    36301 ctccatgaga atacttcccc atgttaaacc tccgccatag cctgtaaata ttactttctg
    36361 ctgttttcct aaggtgatct ttccactaga aactgcttca tccaataaga tagggatgct
    36421 tgcagcagat gtattcccat atttatccat attggtcagg aacttttctt gcggcatctt
    36481 tacttttcgg gcaattttct caataattct gaggtttgcc tgatgcaaaa gcagataatc
    36541 aactggtttg tctttcatga cttcttggat attgtttgca acgtctcgta cggcaaaatc
    36601 aaaaatatct ctgccagtca tttgcaaata atacgaacct tctgaatctt ctgaatagaa
    36661 cggattttta ttttgctggt agccagcggt caatgagcta cttctggtac cgtcagctgc
    36721 taatttttct ttcagcaatt ttttctgtgg attcgcttct aagagaatcc cacctgctcc
    36781 atcgccaaac aacacagcag tcgtacgatc agaccaatcg attagcttag atagtgtttc
    36841 accaccaatc accagtccct tagttgagcc gcagcggatc attttttcag ccatgcttaa
    36901 cgcataaaca aatcctgagc acgcagcact gatatcaaaa gcgatagcat gagttgctcc
    36961 caatttccct tgtacttgtg ctgctacaga tggtgtcgtg taatcgggcg acattgttgc
    37021 tactaaaata aaatccaaat ctttagggtt tagatttttc tgttttatca atttttccgc
    37081 cactaaaatg cataaatcag aagtctcctg atttgttaca catcttcgtt cacgaatacc
    37141 cgtccttgac gaaatccatt catcagaggt atccatcatc ttggaaagat cattattagt
    37201 aataatcttt tcaggaagga cgctggctgt tgcagtaatc attccaaatt gttccattaa
    37261 atatcctcac ttatagtctt gcaagaagtc gtgcaagttt ttcagtgcat ccagcaaggc
    37321 atgctgttcc tccgtagcca ttccgtctaa gatgttcttg accatttcac gatgaaaatg
    37381 ctgatggacc cgaaatagta actttccttt tttcgttagt cccaacttta caacgcggcg
    37441 gtcatcttca ctgcggatac gttcgacata gccttttttt actaacttgt taatagcagt
    37501 ggtcaaggtc ccaacagtaa ttgatagttc tttggctact tctgaagttg tttttttctt
    37561 gtacatcccg attgcctcga tcgtgtgcat ctctgtgatt gacagatctt tgaattgaga
    37621 ttttttcagc tcagactctt cgattgtcaa aatatcattg aagacactaa caagataatc
    37681 atttactgtt tttaaatctg gttccatgtt ccaccgtcca ctaattagtt tgattatcaa
    37741 atcttttgat gttcaaacta tatcttaact tttttcaaaa tgcaacctta aaagatcatt
    37801 tttttactat atgagctttt tttaagattt tactcattta aaacaactga taaatcagca
    37861 tttataattt cttttattag aaaaatcatc tatctcacat attttgatta tcaaagtatt
    37921 ttctgtgaac agaactatag aattccctaa aacttgtcca ttatttcaaa atttgttata
    37981 gtctttttat tcaaagcgta aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc
    38041 ccatgctgcc gggtgtctat cttatgaaag accgagatgg taaggtcatc tatgttggta
    38101 aagcaaaacg gctcaaaaat agagtaagca gctattttca tcagaataaa cagcattcaa
    38161 aaaaagtatt acgtatgatt catcatataa cagattttga tttcgtcgta gtagacacgg
    38221 aattagatgc tttattgctc gagtgtcaat tgatccaaca ttaccgtcct ttttataacc
    38281 gacaaatgaa ttatttcagt aattataatt atgtacatat taccaataag ggatttgtat
    38341 taacagatac tcctacggct cgaacatacg ggccttttcg tctctataaa aaaatgccgt
    38401 ctattttgcg tataatggaa gaaacttatc aaatgccttg gttgtctgaa attagtttgt
    38461 tagctttgcg agtccagctt cctgacctac aggaaatgac ttttgaacag aaaaaaaagg
    38521 aactccaagg gctgtttcaa ggccgaaata aaaagctact gacttacttg aaaaaaagac
    38581 aacaacattt catctatcag ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg
    38641 aattagttac ttattttatt agacggatcc aggaacaaaa gcagttttta agaacaccat
    38701 cgctcacctt cagcatgcca ttagcagcag atgagagtca gaaaaagcac tacctcgttt
    38761 gttatggtca acttgcagaa acaatgatta cctctggtga cgtttctcct gacttctatt
    38821 atgaaaagaa agaagctcat ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc
    38881 ctgtacaaat cttgatcagt taccgcaaaa aattagaaaa agaacagttt gaaattgaac
    38941 tgttaaacaa aaaagaagct gaaaagcaac taaattagtt gcttctcggc ttcttttagg
    39001 catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca tagatttcca
    39061 tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt tcagttaatt
    39121 caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct tctttttcaa
    39181 aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt cgttttatga
    39241 ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct gttatattgt
    39301 atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt ttatgatttt
    39361 ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt tccgtcattc
    39421 tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt tgctggcaat
    39481 acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat aaaatgtttg
    39541 acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt gcttaaagac
    39601 cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt
    39661 ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat catgatctga
    39721 ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa tgtcgtacca
    39781 aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc gaccatgatc
    39841 gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc taacgcatct
    39901 gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc
    39961 aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg tccatagtag
    40021 atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact ttgttctgct
    40081 accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc agcttttgct
    40141 tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg tgcgacagca
    40201 cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc ctaaaaatca
    40261 aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat aaaaaatgtt
    40321 tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg agtcttcttc
    40381 ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa attcgatcat
    40441 agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa cttttggatc
    40501 tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt cgtcggtttc
    40561 aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat ttccttccac
    40621 gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg tttctgaagt
    40681 agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat tgttgtccac
    40741 ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt cctctaactc
    40801 ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag cggagatgac
    40861 ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc tgcttaaaga
    40921 aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc tgatatcctg
    40981 ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag
    41041 cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg gacaaactac
    41101 acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt gcctgatcac
    41161 ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag tatggatcag
    41221 aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga attctgtatg
    41281 ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc acatgcctct
    41341 aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc gaatcacatc
    41401 tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt catgcccatg
    41461 tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga cttgcggaaa
    41521 gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag tccttgacct
    41581 gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg tatacatttt
    41641 taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa ccggcttgtg
    41701 tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat tcttcgtttg
    41761 tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact ggaatatctc
    41821 gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct tctgtctctt
    41881 cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg atctctccac
    41941 gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt ttccggatgt
    42001 tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat tcgggcatct
    42061 tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa
    42121 tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt tctttcattg
    42181 gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc ggcacacgga
    42241 taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt
    42301 tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc gatccgataa
    42361 aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt tttccttctc
    42421 ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg tgcaggatgt
    42481 cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga gcacgttttt
    42541 gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt tcaaaatata
    42601 ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc tacaaactgt
    42661 cactcaaact taagccctat ttttgtctat tcttactgac aaaaacgttt tcttaaaaaa
    42721 gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata cgttgtttat
    42781 tttacatatt catgaacaag ttcaacgact tcttccatcg tatcgcattc tttcaatgca
    42841 cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact acgcgtttta
    42901 agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag caatggaaca
    42961 gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc agcgtgtgct
    43021 gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg gtacaagtaa
    43081 gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc gttcgtacct
    43141 acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc tgctggaatc
    43201 tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact tatcaatttt
    43261 tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt agctaccata
    43321 gggaacatga tacgtaaatt accatgaaca gatgcacgaa gcaatgcacg catttgtgta
    43381 cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata tcctaagaat
    43441 ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc gatgtccatt
    43501 gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta agcttcatat
    43561 tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt acggtacaga
    43621 ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt tccgatgttt
    43681 gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt taatttttcc
    43741 cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc tttttgttca
    43801 tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat atcgccttct
    43861 ttgactttag cagtaatttc ttttgtccca acgattgctg gaatttctaa agaacgtgcc
    43921 atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt tacaaaattg
    43981 cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac cacttcttcg
    44041 ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat acgtttagca
    44101 acgtcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat tgcttcgaac
    44161 ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac gctgttgtct
    44221 ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat caaatgggca
    44281 tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg aatttgctgc
    44341 aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc cgcagtatct
    44401 tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc ttttgcaacg
    44461 gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca gataagcctt
    44521 ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca ccttctgcag
    44581 taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg attgatttca
    44641 agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg cttgctgttt
    44701 gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg tgaaattctt
    44761 tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga gggttttctt
    44821 ttttagtaat tcagagaatc ctaacaaatg ccaccctttc atctgtaaag attaacattt
    44881 tttttcgtta gatacaacag ttttcattca atttcagata aaagtattca aattattccg
    44941 gattcttttt catccttttc ttcacatttt ataacaaacg tgtttatttc ctttttcttg
    45001 tcgctttctc ttctcctgac gtttatcatt ctttcctttg accctctttg ttttcatatg
    45061 aaacgaatta cttgcttccc tttttatttt tagtataata agagtcaaag gtcaggaatg
    45121 gtcaatagag atttcacctt ttctacctca taaatctctt aaaaaactag gggttattct
    45181 tttacctcat gaaaaacgag atgtaaaata tggatgtacc catttttgtg aaaggacgtg
    45241 aatgattcat gctttgtcaa aactgcggca agaatgaggc aacgattcac ttgtatgcca
    45301 ctgtcaatgg tcagcggaca caactcgatt actgtcagag ctgttatcaa aaaatcaaaa
    45361 atcaacaaaa tggaggtctg actaatatgg cccaaaatga tccatttgga tttggcagtt
    45421 tggacgatct tttccgttcc atgtctcgcc aaatgcaaca gcaaggaaat tatgagcaaa
    45481 caccaccaac acaatttggc ggaaacaaca acttcaatgg tggtcagcca cctcaaggca
    45541 atacagaagg tttattggga gaatacggaa tcaatatcac agaacaagca cgacaaggtg
    45601 atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg agtcattgaa attttaaatc
    45661 gccgaaccaa gaacaatcct gtcttgattg gagaaccagg tgttggtaaa acagcagttg
    45721 ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc gcaaaaatta atggacaaag
    45781 aagtcattcg tttagatgtc gtatctttag tccaaggtac tggtatacga ggacaatttg
    45841 aagaacgtat gcaaaaattg attgaagaga tccgtcaagc agaaaatgtg atcctattca
    45901 tagatgaagt tcacgaaata gtcggtgctg gttctgctgg tgatggaaat atggatgctg
    45961 gtaatatttt aaaaccagct ttagctcgag gagaattgca aatggttggt gctactacct
    46021 taaatgagta ccgcatcatc gaaaaagacg ctgctttaga acgtcgtatg caacctgtac
    46081 gggtagatga accaactgtc gaagaaacga tcagtatttt gaaaggtttg caaaaacgtt
    46141 atgaagacta tcatcatgta aaatatactg atgaggccat cgaagcagcc gcaactttat
    46201 ccaatcgtta cgtccaagat cgcttcttgc cggataaagc catcgatttg ctagatgaaa
    46261 caggttctaa gaaaaacttg acgatccaaa tcgttgatcc taaaacaatc gagaaaaaac
    46321 tgcaaaaagc agaagaacaa aaagttttag cttctcgaga agaagatttc gaaaaagcag
    46381 cttattatcg cgatcagatc aataaacttc aaaaaatgaa agaacgtcag ctcactgaag
    46441 aggaaacacc tgtgattacc gaaaaagata tggaaaaaat cgttgaacaa cggacaggta
    46501 ttccagtggg tgaattaaaa gaaaaagaac agacacaact gaaaaactta gcagatgact
    46561 tgaaagctca tgtcatcgga caagataacg ctgtagatcg agtagcgaaa gcgattcgcc
    46621 gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg ctccttctta tttgttggac
    46681 caactggtgt aggtaaaaca gagcttgcga aacaacttgc ctacgaacta ttcggttcac
    46741 aagattcaat gattcgtttc gatatgtctg aatatatgga aaaacatagt gtctctaaat
    46801 tgattggttc tcctccaggt tatgtcggat acgaagaagc tggtcaatta acagaaaaag
    46861 tacggagaaa tccttacagt cttgtcttgt tagatgaagt agaaaaagct catccagatg
    46921 ttttgcatat gttcttgcaa atcttagatg atggtcgatt gacagatgca caaggaagaa
    46981 cggtcagctt taaagatacg atcatcatta tgacaagtaa tgccggaact ggtaaagtag
    47041 aagcaaacgt tggttttggt gcggcacgcg aaggtgttac tcgttctgta ttgaaccaat
    47101 taaacaacta ctttacacca gaatttctga accgttttga cgggatcatc gaattcagtg
    47161 cattatcaaa agaaaacttg atgacaatcg tcacactaat gctagacgat gtcaaccaaa
    47221 tgctggcagc acagcaactt cacatcgaag ttcctacgaa tgtcaaagaa aaattagttg
    47281 acttaggcta tgatccatct atgggtgctc gtcctcttcg acgcacgatc caagaacaaa
    47341 tcgaagatgg tatcgctgaa ttttatcttg atcacccttc tatccatgag ttgaaagcaa
    47401 aactggataa agatggaaaa atcatcgtta cttctaaacc agaacgtttg gctaaagaat
    47461 cagccgaaga aacggctgaa taaatccagt tttatagcgt gagacggtat ttctttgaga
    47521 aatgccgtct cattcttttt tcagaaaagt caaaatactg ttcattttat aaaaaagaaa
    47581 ttataataag agaaacaagc aattaaaaat acataaaaga ggtgagcgcc atgaaattgg
    47641 taaatgtaac aaacagccat tcccgtcttg tattaaacca gcttgaaaac actgatgccc
    47701 atctagtcaa ggtctacaca gccggaaata ccactattgt ctacactgaa gcccctgaac
    47761 ataatgaaat tctgctgatc aatgacaaac gtaaaatcca gccgaaagaa atcgaagatg
    47821 ctaaaaatta tttcttagat aaaatcaaag acgccgttta ccacgaggat gaaatcaaag
    47881 taatcgaatt ggcaggcctt gttgaaatct cgattccaaa acatgaatta ggcgctacat
    47941 tagccgtgta aacaaatttt gaacataata attaaagagc gtgggacaaa agtaaa
//
LOCUS       DAFFFQ010000013        45615 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000013
VERSION     DAFFFQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.013"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            304..1518
                     /locus_tag="LQB69_000827"
     CDS             304..1518
                     /locus_tag="LQB69_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL3525013.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1549..1767
                     /locus_tag="LQB69_000828"
                     /pseudo
     CDS             1549..1767
                     /locus_tag="LQB69_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2186..2659
                     /gene="dfrF"
                     /locus_tag="LQB69_000829"
     CDS             2186..2659
                     /gene="dfrF"
                     /locus_tag="LQB69_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL3525014.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2786..3442
                     /locus_tag="LQB69_000830"
     CDS             2786..3442
                     /locus_tag="LQB69_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL3525015.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3624..4154
                     /locus_tag="LQB69_000831"
     CDS             3624..4154
                     /locus_tag="LQB69_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3525016.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4700..4942
                     /locus_tag="LQB69_000832"
     CDS             4700..4942
                     /locus_tag="LQB69_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL3525017.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            4939..5409
                     /locus_tag="LQB69_000833"
     CDS             4939..5409
                     /locus_tag="LQB69_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL3525018.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            5537..6751
                     /locus_tag="LQB69_000834"
     CDS             5537..6751
                     /locus_tag="LQB69_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL3525019.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            7740..7949
                     /locus_tag="LQB69_000835"
     CDS             7740..7949
                     /locus_tag="LQB69_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL3525020.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            8061..9674
                     /locus_tag="LQB69_000836"
     CDS             8061..9674
                     /locus_tag="LQB69_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3525021.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            9709..10920
                     /locus_tag="LQB69_000837"
                     /pseudo
     CDS             9709..10920
                     /locus_tag="LQB69_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            10943..11869
                     /gene="glsA"
                     /locus_tag="LQB69_000838"
     CDS             10943..11869
                     /gene="glsA"
                     /locus_tag="LQB69_000838"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL3525022.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            12077..13537
                     /locus_tag="LQB69_000839"
     CDS             12077..13537
                     /locus_tag="LQB69_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525023.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            13758..15521
                     /locus_tag="LQB69_000840"
     CDS             13758..15521
                     /locus_tag="LQB69_000840"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL3525024.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            15605..16063
                     /locus_tag="LQB69_000841"
     CDS             15605..16063
                     /locus_tag="LQB69_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL3525025.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            16172..16855
                     /locus_tag="LQB69_000842"
     CDS             16172..16855
                     /locus_tag="LQB69_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL3525026.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            17002..17616
                     /locus_tag="LQB69_000843"
     CDS             17002..17616
                     /locus_tag="LQB69_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL3525027.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            17632..17838
                     /locus_tag="LQB69_000844"
     CDS             17632..17838
                     /locus_tag="LQB69_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525028.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            18379..19509
                     /locus_tag="LQB69_000845"
     CDS             18379..19509
                     /locus_tag="LQB69_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3525029.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            19601..20356
                     /locus_tag="LQB69_000846"
     CDS             19601..20356
                     /locus_tag="LQB69_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3525030.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            20414..22453
                     /locus_tag="LQB69_000847"
     CDS             20414..22453
                     /locus_tag="LQB69_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL3525031.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(22562..23713)
                     /locus_tag="LQB69_000848"
     CDS             complement(22562..23713)
                     /locus_tag="LQB69_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL3525032.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(23867..24397)
                     /locus_tag="LQB69_000849"
     CDS             complement(23867..24397)
                     /locus_tag="LQB69_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL3525033.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(24496..24846)
                     /locus_tag="LQB69_000850"
     CDS             complement(24496..24846)
                     /locus_tag="LQB69_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL3525034.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(25003..26532)
                     /gene="ahpF"
                     /locus_tag="LQB69_000851"
     CDS             complement(25003..26532)
                     /gene="ahpF"
                     /locus_tag="LQB69_000851"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL3525035.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(26542..27105)
                     /gene="ahpC"
                     /locus_tag="LQB69_000852"
     CDS             complement(26542..27105)
                     /gene="ahpC"
                     /locus_tag="LQB69_000852"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL3525036.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(27249..27911)
                     /locus_tag="LQB69_000853"
     CDS             complement(27249..27911)
                     /locus_tag="LQB69_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3525037.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            28095..29741
                     /locus_tag="LQB69_000854"
     CDS             28095..29741
                     /locus_tag="LQB69_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL3525038.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            29732..30814
                     /gene="uxuA"
                     /locus_tag="LQB69_000855"
     CDS             29732..30814
                     /gene="uxuA"
                     /locus_tag="LQB69_000855"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL3525039.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(30922..32475)
                     /locus_tag="LQB69_000856"
                     /pseudo
     CDS             complement(30922..32475)
                     /locus_tag="LQB69_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            32623..33764
                     /locus_tag="LQB69_000857"
                     /pseudo
     CDS             32623..33764
                     /locus_tag="LQB69_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(33890..34969)
                     /locus_tag="LQB69_000858"
     CDS             complement(33890..34969)
                     /locus_tag="LQB69_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3525040.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(35072..35512)
                     /locus_tag="LQB69_000859"
     CDS             complement(35072..35512)
                     /locus_tag="LQB69_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3525041.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(35569..35979)
                     /locus_tag="LQB69_000860"
     CDS             complement(35569..35979)
                     /locus_tag="LQB69_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL3525042.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(36159..36593)
                     /locus_tag="LQB69_000861"
     CDS             complement(36159..36593)
                     /locus_tag="LQB69_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3525043.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(36693..37541)
                     /locus_tag="LQB69_000862"
     CDS             complement(36693..37541)
                     /locus_tag="LQB69_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3525044.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            37930..39696
                     /locus_tag="LQB69_000863"
     CDS             37930..39696
                     /locus_tag="LQB69_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL3525045.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            39798..41057
                     /locus_tag="LQB69_000864"
     CDS             39798..41057
                     /locus_tag="LQB69_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3525046.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            41204..42505
                     /locus_tag="LQB69_000865"
     CDS             41204..42505
                     /locus_tag="LQB69_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3525047.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            42508..43359
                     /locus_tag="LQB69_000866"
     CDS             42508..43359
                     /locus_tag="LQB69_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3525048.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            43372..44187
                     /locus_tag="LQB69_000867"
     CDS             43372..44187
                     /locus_tag="LQB69_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL3525049.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            44338..45504
                     /locus_tag="LQB69_000868"
     CDS             44338..45504
                     /locus_tag="LQB69_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3525050.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT        15459 a         7779 c         8935 g        13442 t
ORIGIN      
        1 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
       61 tctttattcc ttatgaaaaa atatctttaa aaataaaaaa agacgaaaat aaacggtttt
      121 ctataaaaag aagaccgttt attttttgtc tttatttgtg tgaaaaacga aaaagagcac
      181 aacacatcaa gcttttatta attgtctgtc ttttataaat gcaagtatta attcaaaaaa
      241 acagaagaaa aaaacaacta caccttcttt tatactcgta atcgtagaaa ggaggcgaaa
      301 aaaatgaata cattacctat caatacagga gatacagcat tcatgatttt atgtacagca
      361 atggtttgcc tgatgactcc tggtctagct tttttttacg gcggattagc acggaagaat
      421 aatatactaa cgatcatgtc tcaatctttt ctctccgttg ggataacaac gattatgtgg
      481 gtatttggtg gattcggatt ggcctttgga cgggatatcg gcggtgttat tggaaacccg
      541 gcagatttct ttttgatgag acatgttaca agcttcccca acatgtttca tggtgcaacg
      601 attccatttt taatgttctt tctttaccaa ctgatgttta tcgtgattac cgttcctttg
      661 atgacaggag cttttgcagg gagattgaat ttaaaaggct atattatact tgttattttt
      721 tggaatctgc tgatttattt ccctgtttgc cactggatat ggggtggcgg atttttggat
      781 cagatgggat ttagagattt cgctggtgga gcagtcatcc acacgacagc tggattcggc
      841 tctttagcat gtatcctcac acttggtcaa agaaagattc ctttaggaga aagaagtcag
      901 catagcagtc ttatggcagc tgctatcggt acaggactgc tttggtttgg ctggttcggt
      961 tttaattccg gtggtgcact tcgagcagat gaacaagcag tcaatgcatt tggttcaaca
     1021 ttcatcgctc ttgcatttgc gatgattact tggctgatca ttgcaaaagt aaaaggaaat
     1081 ggctttgatt ttgtcgacgt actaacagga tccgttgctg gattggcagc cattacgcct
     1141 tgtgccggtt atgtagaagc aaaaagtgcc atgctgattg ggatcattgc gggtatcgtc
     1201 tgtcatgctg ctgttgattt tcgtaaaaag aaacaatggg atgatgcact tgatgtgtgg
     1261 ggtgttcatg gcatgggcgg attcacaggt acgattctga tagggatctt tgcatctaac
     1321 agccatttac taacgaatcc aagcagctgg tacttcttag ggattcaagt gattggtgtc
     1381 atcattactg ccgtgtatgc ctatgtgctg acaacagtga ttttaaaatc ggcgatgcac
     1441 tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt tttcttcttt
     1501 ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat gcaacaaagc
     1561 aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat gaccatctca
     1621 atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag cttagtattt
     1681 ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc agcagagatg
     1741 gcaacggtga aaggatggga aaaaggtgtt tcccaaacaa aaagatatta agaaacagcc
     1801 catgtttacc tgcttgcaaa caaaagaata ttgctactag actaagagat actacataag
     1861 tgtgttggta tctctttttt gttgccgtga aacagcagac tatttcattt ctgcttgatt
     1921 accttaaaac ttattagtct atggtttgtc aagggcttgt tgcgtgagaa atgctgaata
     1981 tcacccttta caaaacatag gctgataagt aacttttcaa aagcagaaaa gaaatggcaa
     2041 tgcttataac aatatgatat aatgcagatg aagacaaaaa aggaatttgt cgagctggtt
     2101 agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc acaggtacga
     2161 catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt caaagaataa
     2221 tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa agcaatttag
     2281 agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag aaatcggtca
     2341 tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc aaggagataa
     2401 tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac gagatgtata
     2461 catatctggt ggatatggac tatttaagga agctttgcaa atagtagata aaatgtatat
     2521 cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat ttgatatcaa
     2581 tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata cgagaacatt
     2641 ttatgtaagg aaaaaatgaa ttgagtaaat tttggattta gggtgttttc ataaatatat
     2701 tgctttttat gcatatataa attgttgtaa gacaaattcc agtttgtatg gggcgataaa
     2761 atcagaattt gagagaggat tgaatatgaa taaggaatgg tctgaactta ataaaacaat
     2821 gcaggcacaa ataaaaaaga aagatactta taagaggggt attgatactt tacttacttt
     2881 acgaagtcag ttaatacaaa ccttagtatc tttcaaagaa gaattatgca gagaagattt
     2941 taactcaatc cccttcataa acgctgatgg ttatcatagt aagacgattg cttattctat
     3001 ttggcatatt ttccgcattg aagatatagt tgtgcataca gtgataaatg aagatgaaca
     3061 agtatttttt gcaggcaatt atcaagagcg tatcaattca cctatcatta caacaggaaa
     3121 tgaactcatg aaacagcaga ttgcagactt ttcaaaacag cttaatctgg aagaattata
     3181 tttatatatt ttcgaagtat gggaaagcac tgagaaaatg cttgaacggt tatcttatga
     3241 tgaattgaaa agaaaaatac cgaaagaaag aaaaggatac ttagaatcgt tgaatgtagt
     3301 aaacgacaat gaaaaagcaa tttggctgat tgattattgg tgtaataaag atatttgtgg
     3361 actaattcaa atgccgtttt caagacattg gattatgcac acagaagcct gtttgcgtat
     3421 aaagaacaag atacattcat aggcaaaaat ttcagtttgt agaactaagg aaattggaat
     3481 ttaaggagga ataaagtatg gttagtgatt ttataagagc agcatttcca tggattttaa
     3541 tgggtttatt tgtagcaatc agttgttccc ttatgagtaa aaaagagaaa taaattcctg
     3601 tttgtattta taaaagagaa aaaatgagaa aagtagtttt atttattgcc atgagccttg
     3661 acggatatat tgcggacggt aatggtggag tagcttggct aaatggtcat ggaaatgaca
     3721 atgaaaacat cgacacttat acagaattta ccaaagatat agatactgtc ttaatggggt
     3781 ggaatacttc tcatcaggtt gttactgaac tttctccgca agaatgggta tataataaat
     3841 ttacaactta tgtattaaca cataaagagt gcaattcttc ccaacaaatt cattttacaa
     3901 gtgaaaatcc tgttttatta ttggaacggc taaaacaaga agcaggaaag gatatttgga
     3961 tttgtggcgg agcaaatctt gttcagcagt tggtaagcaa aaatatgatt gataaatatt
     4021 acatttctgt tattcctact ctgttaggaa atggggttcg tcttttgggc aacatagaca
     4081 gggaaataaa gttaaggctt tgtaaaacac agacttataa cggaataacc gatttgattt
     4141 atatgcgtag ataactagag agaaacagaa aagctgttaa accgacggag cgatccgacc
     4201 acgttggcgg gaaaatcctt aagatttttc cgccatgctt gcaagggggc ggttcgcttc
     4261 ggcggttcgg cgagcttgcg agccgttcaa agcccccgtt atctaacaag gggggcacaa
     4321 aaaacaagaa acactataca ctaacaagcg gaaaacgctg tatttataag gcaaaacccc
     4381 atttttacag tagtgatata aattgtacct atatcactat gaaaattgaa tagtaaatat
     4441 gagaaagaca gttgatttca aaaaataaga aattgattgt cgttttcttt tgcagaaatt
     4501 taagtgcagg caattatggt agttgtcgta attgctgtca gctcagtgtt tatcactgat
     4561 ggtgtgaaag atttacggtt ggagatgttc aataagatta ttggtgctta attattttta
     4621 ttgtagtgct gatatgagaa tgttataatt ggggtataaa acatacaaat aaatttgctg
     4681 aaaaatgagg tatcaaaata tggaaaatga attactggaa aacttaggaa gacttcacac
     4741 aactgaactt ggcgttatta gaattaaaaa gaacttatct ttgaatgtag aaaatgtaat
     4801 tgaatggtgc aaagaaaaaa ttagcctttc taatgcggaa atcacaagaa aaggaaaaaa
     4861 ctggtatata acgattgata attgtattat aacggtaaat gcgtacagtt ataccgttat
     4921 cactgctcac aaggtaaaat gataaatttt tctataaagg aattaaacgc atttgcagtt
     4981 attgggcagg aagtagaact gactaattat caaaaaaaga atattcagat tagtacacag
     5041 ttttggagaa agtttaacag tagtttgaaa aaagcgtatc tttcacaatc aggaaattgg
     5101 gtaaaatatg cttttatgga aagaagaaat ggaaaacttt attatttctg ttctattccc
     5161 caaagaacca ttaccccaga gggctttctg tataaagaaa taccgtctta taaatatttg
     5221 gttgtggagc atattggtgc tatggataaa atatatgaaa cttatggcaa gatttatgaa
     5281 gaaattattc ctagtacatc gtatattcct ataaaagata ttatcctaca ctttgaaaaa
     5341 tacgattatc gttttcattg gaatagtgat aattcagtta ttgaaatttg gatacctatt
     5401 caagattaga agcaatttta tatccataca cacaaagcag ttagtcttag gattgactgc
     5461 tttttctata cagattttta atgacaggca attatcaatt atggtagttg ccttttttga
     5521 ttggaggaat tgaagaatga atgataaaaa ctttattgaa gaactaagac aaaagcgtga
     5581 ggaatacgga gtaacacaaa caaggcttgc tgttgcctgt ggtatcagcc gtgaatatta
     5641 caaccgcatt gaaaaaggaa aacagccatt gaatgacgaa ttaagagaaa tcatagaaaa
     5701 acagattgag cgtttcaatc cgcaagaacc actattctta ttgattgatt actttcgtgt
     5761 ccgctttcct actacggacg cattggcgat tatccgtgat gtattacaac tgaaatctaa
     5821 ttacatgctt tatgaagatt atggaaaata cggatatgaa agcaaatatg tacttggcga
     5881 catcaatatc atgtgttcca tgcaggagca tttaggtgtt ttattggaac tgaaaggcaa
     5941 aggctgtcgg caaatggaat gttatctgct ggcacaggaa cgctcatggt atgacttcat
     6001 gctggattgt atgacggctg gtggtgtgat gaaacggctt gatttggcta tcaatgataa
     6061 agcaggaata ttggatattc caaagttaaa ggaaaaatac aaggctggcg aatgtatgtc
     6121 ctacttccgt atgcagaaag attacagcgg tacagaaaaa tgcggtagcg atttaccaaa
     6181 gaatacagga gaaaccttat atctcggttc aacaagtagc gaattatata tgtgtgctta
     6241 tcagaaaaat tatgagcagt atgtcaagaa tggcacagaa attgaagata cggagattaa
     6301 gaaccgtttt gaaatacgca tgaagaacga acgagcctat tatgcggttg tagatttact
     6361 gacctatcgg gacgctgaac gcaccgcttt ttctatcatc aatcattatg tccgctttgt
     6421 tgacagagag gacgacaagc caaaaagtca atggataaca aatgatgatt gggcatggtt
     6481 tgtaggggaa aacagagagc cgatacgctt aaccacaaag ccagagcctt acactttaca
     6541 aaaagcgttg cattggctac aaagacaagt cgcaccaacc ataaaaatgg tacaggcatt
     6601 agacagagaa aatcgtacaa cgatactgaa agatatgatt gagcaggcag aactaaaaga
     6661 caagcataaa catttattac aattagagaa atcaaccata gaagaacgta tagataccgc
     6721 tgttccacaa gagaatgacg gtatttttta atgtaattga aaaatttcag atttagtcag
     6781 caaagagctg ttttcattcc ctatgtgaag tgaaagaaga aaaataaatt ttttattaaa
     6841 tctgcaacaa aatggctact tgcgtacaga tatggtgcga gaaaaggaaa tctaaacttt
     6901 ttttgaaaat aggtcattaa atcggggctt gctgtcccta tatgaagtga aagaagaaaa
     6961 aagttttgaa aaaatgacgg aaattcgtga ttagtggaca gtcttctgtg aaaagaggaa
     7021 aaacatcatc attttgtatt caaacaggca ttagcagttg agtgttgatg tgaaaagatg
     7081 aaaatacttt gttctcaact taacaattta aggtttccgt tccctatatg gtgcaaagaa
     7141 aatatttcat tctattgttg gcagtaacgg tatggtttgg gtagttaggg tgtgaaaagg
     7201 aaaatattct tttttcatca tcaacttagc aaaatgacat tttctatccc tatatggtgc
     7261 atgaaaagaa gttaaaattt ttccatttaa acttagcaaa tggacgtttg ctgtacagat
     7321 agtagtgaaa aaggagaaaa tcaaccgttg attgccaaaa tgaaccgttc catttgtact
     7381 aataatggaa aaagttttgg attttggtta cgtttctcct cttttccaac gcgatatata
     7441 ggaaagacga tttttttctt tcaatcgttc tttgacaact gaataaagct attcaatacg
     7501 tttgacagac agctagccgt tgaaacaaat acgccacgac ctttcccctg caagagcgag
     7561 cgaaaaccta tttgtgttct tgtaaaagat ttgtcctctt ttatcgccat gacctgtact
     7621 gtcagataat gatactcccg tacagccaca gcttgagcat taagagcgtc gcacgcaatg
     7681 ggtacggcta cataagaact atgcagaggt ggaactcctg tgggctatga caaaagccgt
     7741 tgaattgctt agaaagattt tacagaaagg gggtatgtgt tattgataat gctgtaaaaa
     7801 caattcaaaa gaaaaatggc aagtgtggca ttggcaacag aggaaagaca aagattgtag
     7861 taaaagagca tttttccgaa aagggcaaaa caatggaaga acttctaact gatgtcatgc
     7921 tggaaaaagc aaagcagaca atcgcataaa atcggtgcag ttgtaagaaa tagattgaat
     7981 gacaaaaagt acccgtgtta tactttactt gcaagcaggt attgtaaaca cgggttagtt
     8041 ataaaggagg ataactgaca atgaaacaac agatttacaa tactgcactt tatcttaggt
     8101 taagccgaga tgatgaatta cagggcgaaa gttccagcat taccacacag agaagtatgt
     8161 tgcgtttata tgcaaaagaa catcatttga atgtcattga tgaatatatt gatgacggct
     8221 ggtcgggaac aaattttgac agaccgagtt ttcaaagaat gattgaggat atagaggcag
     8281 gaaaaatcaa ctgtgttgta acgaaagacc tttcccgtct tggcagaaat tatattatga
     8341 caggacaata tacagaattg tatttcccta gtcataatgt ccgctacata gcgattgatg
     8401 acggtgtaga cagcgaaaaa ggcgaaagtg agattgcacc atttaagaac atcatcaatg
     8461 aatgggtggc aagagataca agccgtaaag tcaaatcagc atttaagaca aagtttgcgg
     8521 agggggctca ttacggtgct tatgctccgt taggatataa gaaacaccct gacatcaaag
     8581 gaaaactgtt ggttgatgat gaaacaaaat ggattattga aaaaatcttt tccctagcct
     8641 atcaaggtta cggaagtgca aaaatcacaa agcagttaag agcagaaaaa gttccgacag
     8701 cgtcatggct aaattttaca agatacggta cttttgcaca tatctttgag ggaaaacccg
     8761 aaagtaagcg ttatgagtgg acgattgctc atgtcaaggc gatattgaaa agtgaggttt
     8821 atatcggaaa cagtgttcac aatatgcagt ctacggtatc gttcaagagc aaaaagaaag
     8881 tgcgtaagcc cgaaagtgaa tggtttcgag tagaaaacac tcacgagccg attattgaca
     8941 aggaagtgtt ctatcgtgtg caggagcaga taaaatcaag acgcagacag acaaaggaaa
     9001 aggcaacgcc gatttttgca gggcttgtca agtgtgcgga ttgtggttgg tctatgagat
     9061 ttggaacgaa taagacaaat aaaacgcctt acagttatta tgcttgcagt tactacgggc
     9121 agtttggcaa aggtaattgt tctatgcact acattcgtta tgatgtgctg tatcaagccg
     9181 tattggaacg cttgcagtat tgggctaagg cagtacagca ggacgaagaa aaggtattga
     9241 acaagataca gaaagctggc aatgcagaga gaatacggga aaagaagaaa aaggcaagta
     9301 cattgaagaa agccgagaac agacaaaatg agattgaccg tttatttgcg aaaatgtatg
     9361 aagatagagc ctgtgagaag ataacagagc gaaattttgt gatgttgtcc agcaagtacc
     9421 aaaaagaaca gatagaactg gaacagcaga taacaagcct aagagaagaa ctaagtaaaa
     9481 tggaacagga tatgataggt gctgaaaagt ggattgagtt aatcaaggaa tattccgtac
     9541 caaaggaact gacagcacca ttattaaatg ccatgataga aaaaatcctc atacatgaag
     9601 caacaacgaa tgaggataac gaaagaatac aggaaattga gatatattac cgatttattg
     9661 gaagagttga gtaatcaaca agagtaatat ttttaactaa gggaaaccgg cggtgtctat
     9721 acttgggtca gtcgcacatt gggaaaacga ttcggttttg ccgcaatctt tttccaatgg
     9781 tttgagataa cagttggcta cttgaccatg ctgtatttct taactggtgc attatcctat
     9841 gcaacgggta ttggtgctat acaaaacaat aaatatctga aattggcaat cctattgatt
     9901 attttctggg ctattttact ctcacagtta aaaggaacca aatacacttc attgattgct
     9961 cggatcggtt ttgtagctgg tatcttgttg ccagcattag tcttgtttgg cttagggatc
    10021 cattatgtcg cttcaggcgc tccactacaa acgatactgt ctatgaaaac attgattcct
    10081 gatttttcga aattgcctac gctggttgtt tttgtttcct ttattttagc atatatgggc
    10141 gtagaaacat ctgccagtca tgcaaatgaa atggaaaacc ctaaaaagga ttatccatta
    10201 gcaatgtttg tattggttat tctggcaatc attttagata cttttggcgg attgaccgtc
    10261 gctactacag tacctcaaca tggtttgtct cttaatacag gggtgattca atcgttagaa
    10321 ttcctattaa gacacattga tccaaatctt gcatggattg cgaaaattct tgcgattctt
    10381 gtttgtttag gtgtcatcgg agaaattgct tcttgggtaa cctctccttc aaaagcactt
    10441 cacgtagcag cagaagaagg attacttcct gaatattttg caaaagaaaa cacacacggt
    10501 gttcctgctc atttgatgat cgcaaatggg atcgttgcaa caatctgggc agccgtcttt
    10561 acattaagcg gcggggggaa taatctttca tttcttgcag cgatgtcatt gactgttgta
    10621 atctacctga tgttgtactt tatgtttttc gcgggctatt tgaagctggt ttttaaagat
    10681 gacagtcttg aaagagccta tcaggttcca ggcggaaaag ttgtaaaagt aatcgttgct
    10741 tgtttaggtc ttttgacttc actttttgct ttttgtatct catttgttcc acctgctagt
    10801 atcgcagctg gacagcatgg gatttatgaa acgattctga ttgtttctta tcttatcgtc
    10861 ttagttattc catttacgat ctacgcatgc cgtcataaat tccagcatgc agaaaaataa
    10921 aaaatgaaag ctggttattc aaatggaagt aaaagaatta gaacaattga ttggaaaaaa
    10981 tttccactat tatcaaacag gacaagtggc ggattacatt ccagcattag ctgaagtgga
    11041 tcccaaacaa ttaggtatgg cgatttatga tttggataca aaacaactga ttgagggagg
    11101 agacgccgat gtgcgctttg ccatcgaaag catgtcaaaa gttcccgtat tgttattagc
    11161 aatcaaagac aacggaatag aaaaagtctt tcaaacaatc aacacggaac caaccggttt
    11221 tgccttcaat tctatcatga atatggaaat caatcatcgc aaacatccaa tgaatccttt
    11281 tgttaatgca ggagcgattg caacaacctc gttgattgcc ggtaaagatg cagatgaaaa
    11341 atttgagcga atcttgtcat ttatgaaaga gatctgtgat gatcctaata tcacattgaa
    11401 tgaagaaatc tatcattctg aatcaagaac aggtgatatc aatcgctctc ttgcttacta
    11461 tatgaaaggt aatcaaatga tcgaaggaga cgtaccggag atactcgatg cttatttcag
    11521 acaatgctcc ctcaatgtca ccgctacagg catagcaaaa ttagctgctg tgctggcaaa
    11581 taaaggaatt gctccttgga atggcaaaca aatcattact gaagaaagtg ctacaatcgt
    11641 caaatcaatt atgacaactg ctggtttgta tgatgaatca ggagagtttt ccgtccatgt
    11701 tggtgtacct gcaaaaagcg gtgtaggcgg cgggttgatg gcagctgtgc caaatcgtta
    11761 cggcataggt gtcttcagcc cggcactaga tccatttggt aacagcgcag ctggtatcca
    11821 attgttgaaa gatgtagtca aagaactaga cgctgacatc tttgaataac cattcttgaa
    11881 agaggctggg gtaaaagtcc aaacggctca aagctaccta caatgaggct gaaattgtga
    11941 gcttgtttga cttttgtccc agtttctttt tttattggtt tttgaaaggg cttttctaaa
    12001 taaaaaggaa acaaaaatac gatttacaga tatgattaaa taaaatcata agaaaaactg
    12061 gaggcgtgta tcaggaatgc tggaaaatta catcgagcga aatatattta gaaaagtgta
    12121 tctgtgtgaa caattatttg aattccaaga aatagacatc gaacaaacag ctatttctct
    12181 aagggtgact acaccaacga tccttcacga tctagaaagt ctagccgagt gtctagaata
    12241 ttgtataaaa gaacaagtaa gagagaagca taagtataaa ctcgtattca aacatggtat
    12301 tgctctttct gagctcacac agtttctgta cggtcaatct tattttttaa agtttttaag
    12361 ttattatttt aatggtatat tcacgagtac agaactggct gatcttgaat ttatttcttt
    12421 gagtaaagta tatacaatca aaaaaatcgt attggatttt tttaaagaaa atgattattt
    12481 gaaaaataaa gaaatcatta tcccagaatt tgactcgcgg aacatcttgc tagctttagt
    12541 acgttatgtc aattgggaag gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa
    12601 cgagttgatt gattatgtag agactcattt ttttaaaaga agatattcgg aagatgaaaa
    12661 gtcctttatc atacgagaaa tagagatcgc aatcggaaga aaggcatttc cccttggact
    12721 gaaagaagaa gataagaaaa gcgcagaaac gaagccgtta tttcaattag tctgtcaagg
    12781 attggaagag caaaaaggaa ttctagactt acaagaagaa gatgtctatt atattttttc
    12841 tttgttcaac agtagaaatt ataccaatga gaacatggag ctgttaagaa aagatttcga
    12901 agttgtttat aaaaattttg ttctcaatga cctatcactg aatgaattga ttttacagat
    12961 tattagtatc gctgaaatcg acgatgcaga aaattttatt ttcaagcaat cctttttaca
    13021 atttgtccgt acgttgtggg cagatggaca agtattttta ccggaaagga tctatcttct
    13081 atcagaaaaa gaacaacttc tgtacggaaa aattgtagaa atattggaag agtggaaaaa
    13141 gaaaaatcaa atccaactaa gatggaatga aaatttcatt cgaaaattcg tcaaaagtct
    13201 ttctttgatc aacagtgaac aagcggggag acaggaaata gaaatttttg ttgtttcaga
    13261 aagttcagtc aaacaatttt tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat
    13321 cgctgaaata aatcctattt tatttcgttc attagaagaa ttgcctgatg aatgtctatg
    13381 tgcgaaaaaa agagtggttt tgtgcgatgc tgctagctat caaaatggat tagaaactga
    13441 aaagacaaag atctatccaa tctcattaaa aggtgcaaac atccatttgt atcatattgc
    13501 tcaggaactt cacctattaa aaaaacatat ttcttgatga gaccttaatt aggagacctt
    13561 ctacaaagct ttttaattgt ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga
    13621 tctacttata taacaagctt tctctgaaat ttttttaaaa tgctattgac ttagagtcaa
    13681 ctctaagtag tagaatagat aaagtgtaac aaatctattt tcattaaaag aagatagtac
    13741 ggaggagaga atgtattatg agtacagtat tacatgtatt ggatgcaatc aatagccccg
    13801 cagatgtcaa aaaattatct gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa
    13861 aaatcttgca aaaggacagc gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag
    13921 aattaacgat tgcgtttcat tatgtatttg attcaccaaa ggataaaatc gtttgggatg
    13981 tttcccatca gtcttatggt cataagcttt taactggtcg aaaaaatggt tttgaaaaag
    14041 gacattaccg tgaagtgagc ggatattcca accaacatga aagtaaacat gactatttta
    14101 cagtggggca cacatctaca tctatcagct tagcaacagg tttagctaaa gcgagagatc
    14161 tgaagaaaga atcaggaaat gtggtagcat ttatcggtga cggctcccta agtggtggct
    14221 tagcttttga aggattgaac aatggtggtg ttttacaatc caatctaatc gtattagtaa
    14281 acgataatga gatgtctatt gatgagaatc atggcagttt atataaaaac ttagctgagc
    14341 ttcgtcaaac caacggtaaa agcagcaata acctatttaa agcaatggga tttgattatc
    14401 gttacgtgga taatggaaac gatctggaaa cggtgatccg tttatttgaa gaagtaaaag
    14461 atattgatca tccgattgta cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg
    14521 caatcgataa taaaatgaaa tatcattggc atgtaccatt tgatctgtcc accggggagg
    14581 caaagcaagt aacttctgga gaaacattta atatgatcat catggattat ttggataaga
    14641 aaatgcaaac aagtacagct cctattgccg cagtcaatgc agcgatacca ggcgcctttg
    14701 gactgaaaac attccaagaa aaacatccag atcattattt tgacgtgggg atcgctgaac
    14761 agcactctat cactttcgct tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc
    14821 aatcaagtac tttcttgcaa cgggcatatg atcaattatc acatgattta gcgatcaacg
    14881 agaatccagt gattatttta gtcggtggcg gaacgatctc agctagtgac cgaacacacc
    14941 aaggaacatt tgacattcct tacttatcaa gtatcccgaa catcaaatat cttgctccag
    15001 caacaaaaga ggatatgatc tctatgctag attgggcgtt cgaacaaaca gcgcagcctg
    15061 tagcaatccg aattcctaca catggtgtag aacatggtac agctatcgca aatgattata
    15121 gtattccagc ctatcaaaca gtaaataatg gaggaaaagt agcaatttta gctttaggcg
    15181 gttttcttga attaggaaaa gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa
    15241 cgttgatcaa tccaggaacg atcacagaaa atgacgtaga aacattagat aaattaaaag
    15301 aaacacacga attagtcatc acattagaag atggtagttt gtcaggagga tttggcgaaa
    15361 atatcagtcg ttattatggt gattcttcaa tgaacgtatt aaattttggt gcagagaaag
    15421 aattcacgga tagcgttcct gtagggcagc tttatgaacg ttatcattta acaccaaaac
    15481 aaatcgttga agatagtctg cgtgtcttga atattcaata attcattatg ctcaccatcc
    15541 agataattgt ttatactagt atctggatgg ttttttcatt ataaaaagca aaggggtgga
    15601 tgttatgtat cagcaagtag aagaatatgt caaaaagttc caagaaaaat atcctagtta
    15661 tcaatcagaa aaattaggtt tcttttcatt tggggataca gaaaaaatgg cagacgagtt
    15721 gagcgaacta gttgtaaaaa agataaagac tggtacatca agtggttacg atctgtataa
    15781 acaagagaat gagcctttac caaaagttgg tcaattagac gtggtcctga atggaaaaaa
    15841 tgaaccagtt tgtatcatca aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac
    15901 gaaagagcat gcttggaaag aaggagaagg ggatcggaca ttagcttatt ggcggaaagt
    15961 ccatgctgag ttttttaaac atgcttataa agaagcgttg aacatagaat ttacagaaga
    16021 gaagctggtc gtatacgaag aatttgaagt aattttcttt tagtaagaaa ggtctgctga
    16081 aagaaatagc tcattattgt ctcctgaata gaaaagggtt acatttttat atgaagctgt
    16141 atttttatat aacattactg gaggagatat tgtggataca ctggaacaaa tcaaaggcgg
    16201 agtcgtcgtt tcttgtcaag ctttggaaaa cgagccgcta cacagttctt ttattatggg
    16261 gagaatggct ttggctgcaa aagaaggcgg agcagttggt attcgagcaa atacgacaga
    16321 agatatcaat gaaatcaagc gaatcgttga tttaccggta atagggatta tcaaaagaga
    16381 ttacgatggg tcagaagtgt ttattactgc gacagaaaaa gaagtggaag aacttttgga
    16441 gacgcaagca gaaatcattg cactggatgc aacttctcgt aaacgtccga cagaaaaaac
    16501 aactgctgaa cttgtccaaa tgattcacga acacggtcgg ttagctatgg cagatatttc
    16561 gacttttgag gaagcgcttc aagcgcaaga agatggtttt gatcttttgt ctacgacttt
    16621 agcaggctat acagaatatt cacgaaagac gcagacacct gattttgatt tattgaatca
    16681 aatcatagaa caagtcgata tcccagtaat tatggaagga catactgact ctcctgaaca
    16741 agtagagaaa gcatatgaat taggtgcttt ttcagtcgta gttggatcta ttatcacccg
    16801 tccgcaattg atcactaaaa gatatgtcga agccgcaagt aaagcaaata actaaaaaac
    16861 acgcacggca cagattcttg acaaaaaaga aaaattgata gatagtttaa agcagattct
    16921 aagaagatga aaaaagcatt acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt
    16981 ttatagaaag aaggcgaaac catgtttgaa cggagtcctt cagaattgac agaacgtgaa
    17041 aattacaagt tgctgattgg ttcagtcatc cctagaccag tagcagtagt aacgactaaa
    17101 tcagcaaaag atatagtgaa tatcgcaccc ttcagttact tcaatatcgt cagttcgaat
    17161 cgacccatct tatccttggc gatccagaga aaacaagatg cagtaaaaga cacagcaaag
    17221 aatattcttg agacaagaga agctgttatc catatccttg atgaggacaa cgtagaagaa
    17281 gtaaataaaa cagcagccaa tctgcctaat gaggaaagcg aactgtacct gactgacttg
    17341 actttaactg attcagtaat catttcagta ccagcattga cagaagcaaa agttcgtttt
    17401 gaaacatcct tatatgaaca tgtcgagata cggaataaaa ataaagtaac agctgattta
    17461 tttttgctag aagtacagaa atatcattta aatgaaacag tctatgatga taagacagga
    17521 aggatcgatc caagaaaatt gaatgctgta agccgtttgg caggaaatga ttatgcagca
    17581 ataggagaaa ttttcacgat tgcacgtccc gaataaaaag gagcactagt tatgttaaaa
    17641 ataagctata aaccatcgac agattcaaaa gaaatgaaaa aagaatatga aacagtgaat
    17701 gattttctgc aagggcagta cttagaagtt ccgccattgc aagaccattt tgtcgttact
    17761 actgttactt tagatggtaa ggaaatagaa atgccagatc aaacgattag cggattgttt
    17821 aattacttta acaaataaat atttattccg aaaagtgaaa aaacatataa aaaaacacat
    17881 aaaactaaaa aattatcaaa gaatcctggt gcaactgaaa gaatttgcaa cgggattctt
    17941 tcaacttatt tgttattttt ttctaaaaaa tattaaagat tgttaaattt cactatttca
    18001 tttcaaattc cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat
    18061 agtaataacc ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg
    18121 aaagtcgcca atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa
    18181 tgttttcttt acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat
    18241 tcttgttcga aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga
    18301 gacaaactcg ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa
    18361 aagatcgatt ggtggatttt gggtccttat ttgactttgt ccatgatcgg tcttttagaa
    18421 gtatacagtg ccagttctta tcgtttattg caagccgatg aaaatacaaa aagtctatta
    18481 ttgcgtcaac taattttcat atttttgagt tggagcgtga tcttcttagc tcgttcagtc
    18541 aaactacact atttacttca ccctaaaata gcaggatacg gtttagcctt atcgattttc
    18601 tttttagtat tagtaagaat agggatattc ggtgtcactg tcaacggcgc acaacgttgg
    18661 atctctctgt ttggcattca attccagcct tctgaactgg caaatctttt tttgattttt
    18721 tatttaagct ggttttttcg tgacggaaat agtagcccaa aagatctaaa aaaaccattc
    18781 ctgattacag taggtataac ttttctgatt ttatttcagc caaagattgc tggagcattg
    18841 atgatccttt cgattgcgtg ggtcatattt tgggcagcgg cggttccatt taaaaaaggg
    18901 atctatctaa tcgttacttt ttctgcattg ctgattggag cagcaggcgg ggtattatat
    18961 ttaggaaata aaggttggct tccacagatg tttaatcatg cttatgaaag gatcgcgact
    19021 ttaagagatt cttttatcga tagtcatggt gcgggctatc aaatgaccca ttcgttttat
    19081 gcactatata atggtggcat atttgggcgg ggattaggaa atagtatcac aaaaaaaggg
    19141 tatcttccag agacagaaac agattttatt ttttcgatta ttacagaaga gcttgggcta
    19201 atcggtgcat tgtgtgtgct tttcttattg ttctcattat gcatgagaat cttttgttta
    19261 agcagtcgct gtaaaaatca gcaagctgga ttgtttcttt taggattcgg aactttgcta
    19321 ttcgttcaga cgataatgaa cgtgggaagt atcgcggggc tgatgccaat gactggtgtg
    19381 ccattgccat ttgttagcta cggaggaaca agttatctga tcctttcttt aggaatcgga
    19441 atcacgttga atatttcttc aaagatacag gccgaagagt tggcgctcta tcgacctgaa
    19501 aaacaataaa gagaaactga gaaactctca gtttgaatta tcataaagta caatctttta
    19561 tttttcaagc gagaggttct tttagaaaga ggaattcgca atgaaagtat ctcaaaaaat
    19621 catattatct attttgttac tgcttacatt agtagttggc tatttttcga ttgagtttat
    19681 ccatggattt tcgtctgcaa aacagagttc aactgtgaaa aaggtagagc cgaaaagtat
    19741 ccctaccaca ctaaatgtgg ctttgattgg ttcagatgct cgttcgaaag acgaaaacgg
    19801 tcgctcggat tcacttctgg ttgcacaata caaccagaaa acacaacaag caaaactgat
    19861 ctctatcatg agagattcat atgtcgatat tccaggttac ggaatgaata agataaatgc
    19921 agcttactct tatgggggag ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt
    19981 tgaagctccc tattatgcaa gtatcacatt tcaagatttt atcgattgcg ttaatgaact
    20041 gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta gatttagatg gcgtatatat
    20101 aaacaaagga gagcaagtga tggatggtaa tactttactc cagtatgctc gatttcggga
    20161 agacaaagaa ggagacttcg ggaggatcag aaggcagcag caagtgatca aagctatctc
    20221 tcagcagctg aaagacgtaa cttcaatatt caagctaccc aaagcggttg ggaaattact
    20281 tggtagtatc caaacgaacc ttccagaaag tgtgcttctg gattgtggta tggatttcct
    20341 caaagacata tgttagaata aacaggtata aatagtgaaa taaaggaatg acaagcaaga
    20401 gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat
    20461 tgccggcgca gtgatcttaa tagcagctgc gggtggcggt tatttttact accagcacta
    20521 tcaagaaacc caagcagtag aagctggaga aaagacagtt gagcaatttg tccaagcttt
    20581 gaacaaggga gattataaca aagctgcaga aatgacatca aaaaaggcgg caaataaaag
    20641 tgcattatct gaaaaagaga tcttagataa ataccagaat atatacggtg ctgctgatgt
    20701 caaaggactt cagatatcga atctaaaagt agataaaaaa gacgattcta cttacagttt
    20761 ttcatataaa gcaaagatga atacctcatt aggtgaattg aaagatcttt cttataaagg
    20821 aacattagac agaaatgatg gtcaaaccac gatcaactgg caacccaact tggtttttcc
    20881 agaaatggaa ggaaatgaca aagtaagcct gaccacacaa gaagcagcaa gagggaatat
    20941 tatagatcga aatggtgaac cattagcaac aacaggcaaa ctaaaacaat taggagtcgt
    21001 tccaagtaaa cttggagatg gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc
    21061 cttcgactta acagaagatg ctatcaatca ggcgatttca caaagctggg tacaacccga
    21121 ttactttgtc ccattgaaaa tcattgatgg agcaacgcca gaacttccag ctggagctac
    21181 cattcaagaa gtagacggca gatattatcc tttgggtgaa gcagctgctc agctgattgg
    21241 ttacgtggga gatatcacag cagaagatat tgataagaat ccagaattaa gcagtaatgg
    21301 aaaaatcggt cgctcaggtt tggagatggc ttttgataag gatcttcgtg ggacaacagg
    21361 tgggaaatta agcattacag atgcagacgg tgtagagaaa aaagttctga tcgaacatga
    21421 agttcaaaat ggcaaagata tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt
    21481 tgacagtcta ggaggaaaag ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct
    21541 tcttgcgctt gctagctctc caagctatga tccaaacaaa atgacaaacg ggatctcaca
    21601 agaagattac aaagcttatg aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac
    21661 aggttatgct cctggatcta cgtttaaaat gattacagca gcaatcggtc tcgacaacgg
    21721 cactatcgat ccaaatgaag tgttgacgat caacgggctt aaatggcaaa aagacagttc
    21781 ttggggatct tatcaagcaa cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac
    21841 tgctttgatc tattccgata atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa
    21901 aaaatttcgt acaggcttag ataaattcat ttttggtgaa gaccttgatt tgccaattag
    21961 tatgaatcca gcacaaattt ctaatgaaga tagctttaac tcagatatct tgctagctga
    22021 tactggatat ggacagggcg aacttctaat tactcctatc cagcaagcag caatgtattc
    22081 tgtttttacc aacaatggca cacttgtcta tcctaaattg attgcagata aagagacaaa
    22141 agataagaag aatgtcatca gcgaaacagc agtacaaacg atcgtgccag atctgagaga
    22201 agttgtgcaa gatgtaaatg gtacagcaca ttctctttct gctttaggga ttccgttggc
    22261 agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat gtaaaggggc aagagaacag
    22321 tttcttgttt gctttcaatc ttgataacca aggttatatg atggtcagca tgttggaaaa
    22381 taaagaagat gatgattcag caaccaaacg agcatcggaa ctattgcaat acctcaacca
    22441 aaattatcaa taaaaaattg ctttttaagt acagtaaaag gactgtggcg ttatcgtcac
    22501 agtccttttt tcaaataaac ggttttcacg atgtctcttt cacaacttct ttttgattta
    22561 ctcacacttc ataaactttt tttgtaaaat atttgagaac aagtggagaa ataagtgtac
    22621 ttaataaaat cacgataact aaaggtgaat agtactgtgg ttcgatcaat tgactttgct
    22681 gtccaatttg taaaatgatc aaagccattt ctccacgcga aatcatacca gcaccgacca
    22741 tcaatgaact atgttgagaa aaaccggcaa tctgagcacc aaaataacct ccaagcaatt
    22801 tggtcaaaat cgctacaagc gtcaatatag aaataaagag tagttgttca ctaaagtcag
    22861 aaaaatcaac ttctaatcct acactgacaa aaaatactgg tataaatact gcatatccta
    22921 aagcctctac attgtggtat acttcttctt taacctttgt ctgactgaca gctatcccag
    22981 caaaaaaagc accaatgaca gagctaagcc caacaagatc cgctacgtaa gacattccta
    23041 aacaaataac tagcgatacg atgataatag aagaatttgc gtacactttt tctgcaaatg
    23101 aaagtaggaa aggcgcaatc catttgacta ataagaaaat aaaaacaaaa taaattagct
    23161 gttcaatcaa aacaagcggt aaagaagcat tgctagaagc ttcccccgtt agaaaagata
    23221 ggctgaaact tagaactaaa acgactaaaa tgtcatctac cacagaagca cccaaaattg
    23281 ttgatccttc ttttgtattc actacgttta gttcttttaa tacttctact gaaatactga
    23341 cagaagttgc cgcaagaata atcccaaaga aaatcgcttc atttcccgaa acttggaatg
    23401 ctttgcctgt caaccagcca aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca
    23461 taccaggtcg gaaatatttt ttcaaaagag ataaatcact ttccagacca gctaaaaaca
    23521 ttaataagat cacaccaatc tccgaaaagt catggactag aatatcaggt ttgacccaat
    23581 tcaatccagc ttggcctagc aaaacaccaa caagtaattg tccaatcact gctgggatac
    23641 ctaatctccg cgagatatgt gagccaatcg ttgttgaaat caatatcagg cataaaatac
    23701 caataaattc cattctcatc attcctttgt ataaaatata gcccgaactt agcataacat
    23761 acattattga aaaaatagga aaatatactc acaaaaaaac caacaatcca aaagattttt
    23821 ttggattgtt ggtttttata cggtctattt taataactaa aaatgattaa cgtatagtca
    23881 tttttcttat tctgatgaaa ttgataatct cctggatgcc catgaaaatc aaaataaagc
    23941 cgaatatttg gaatagaagc atcaaacttt tgaatggatt gaacaataag acgaatccgc
    24001 caatcaacaa cagaatacta ctataaacac tccaactttt agcttttgta ttcatgctga
    24061 agaacaactg gaacaagctg ttgatgataa cagctaatcc aagaagaatc ggcaggatag
    24121 aaacgattgc aggtgcaaaa gcatagacga tcagagctaa gatcaggaaa aaaagaccac
    24181 tgaacagacc gatactccaa gaacctgttt tccttttaaa acggtaagcc tcaaaaagat
    24241 tcaacaaccc aagcaaaaca agataggcgg tgatcagata accgataaaa tgaaaaacag
    24301 cagaagggtt tagcacaatc gcaatcccag aaataatata aacagcagct cttaataagg
    24361 cgtatttttg aatattttgt ttaatcgtct caaacatata agaaaacctc ctttgactta
    24421 ttttaacgtg aactagtcat tttaccaaat taaatgttac aaaatgagcg aaggagaaga
    24481 atatctctgg aaaaatcaac ttctgaggat cttttccatt tttttgcctt ttgctagctc
    24541 atcgatcatt ttatctaaat aacgaatttc tttcatcaag ttatcctcga tgtcttctac
    24601 tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc
    24661 cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga tattctagtt ctgtttggga
    24721 ataacccgtc agccaacaaa tgatttcatc tacttcctct tttatacgat cttttctctc
    24781 cgcttttgct acatacatag gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg
    24841 cgccataatc agtcaccttt ctttttttca aattatttac tacttatagt atataagaaa
    24901 aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac
    24961 catccttttt tccgctctct gactttttac aatcttcata gctcaagtgc gcatcagata
    25021 atcgaatgct cctaaagctg cagtagctcc tgaacccatt gaaacaatga tttgtttgta
    25081 tggactatct gtacaatcac cagctgcgaa aacgccgggg atattcgttg cgccattttt
    25141 ctcggtcact atttcatttc ttggattcat ttcgatcgtt ccatctagcc aatccgtatt
    25201 agggagtaag ccgactagaa tgaatactcc tgaaacagct agtgtatgca tctccatcgt
    25261 acggcgatcg atataggaga gagattctac ctgattcgta ccgctgattt ctttcgtcgc
    25321 tgcatgtttg ataacagtga cattcgcaag agagactagt ttatcctgta agatttgatc
    25381 tgcttttaat tctggtaaga attctagaac atatacatgt tttgctaacc cagccaaatc
    25441 gattgctgct tcaacaccag aattaccacc accgataaca gcaatctctt ttcctttaaa
    25501 taaagggccg tcacagtgtg ggcaataagc aattccttta tttttaaact ctttttcgcc
    25561 tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag
    25621 tgatataccg ttagctaagc caatattaat aaaatgttgc tctttattta aggaaacggc
    25681 ctgttgattc atcataatgt cgatagggta actacgcaca tgactttcta cttgctgcat
    25741 caattttgga ccttctgtgt aagaggtacc aatcatattt tcaatcccta gtgtttcaac
    25801 gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg attcctttac gtgctgcata
    25861 gatagctgca ctactagctg ctggtccacc gccaatcact aatacatcat aaggttcttt
    25921 ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt tcaacaattt gttcgatcgt
    25981 catgcgtccg ctactaaatt cttctccatt taaaaacaca gttggcactg ccatgatttt
    26041 tttatcatcg atttcttttt ggaacatccc accttcaatc atcgtatgag taatatttgg
    26101 atttaataca gctaatatat tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa
    26161 gctaacataa ctgtcaaaat gcaatgtttg tgttattttt ttgattttaa gactcaaaga
    26221 ttcactgatt ttcggtgccc gtccactaat ttgtaacata gccagtaaaa aagaggaaaa
    26281 ttcatgaccg agggggatac cagcaaaaac gattccactt tctttgccaa ctggattgat
    26341 agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa gagattttag gagacatttc
    26401 tgctatttcc ttcgtaaatt cagctagttt ttgactattt tcactattat ccaaactaac
    26461 agaaaaaaca ataggagatt caatcagtgc taaatattgt tcgagatttt gtttagtttc
    26521 tttgtctaac atatccacca cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt
    26581 ttcgccactt tctttccatt ttgccggaca aacttcacct gggtgggtac gtacatattg
    26641 agcagcgcga atcttatcaa tcagtgcact agcgtcacga ccgattccat ctgcattgat
    26701 ttccatagct tgaacgattc cgtctggatc aataataaat gttcctcgct gagctaatcc
    26761 ttgctcttca tccaaaacat caaaaatacg agaaatctga tgagacggat caccgatcat
    26821 cacgtattcg attttaccga tagcatctga tgtatcatgc catgctttgt gagtaaagtg
    26881 ggtatcggta gagcaagaaa agacttcaac acctaaagat ttcaatgtac tgtattgttc
    26941 ttgtaagtct tctaattcag taggacaaac aaaagtaaaa tcagctggat agaagcaaag
    27001 aatactccag ttccccataa gatctttttc actgacttct aaaaattctc cttgatgata
    27061 agcatgtgca ttaaattctt cgattttttt cccaatcaat gacatagtaa aacctccaat
    27121 aataattatt tatttataat tattgtaatt tagttggagt tgcaaagtca atggatattt
    27181 tgttatttaa taaagattac tcaaaaacga aggttaatta taaaaataga agaggtcgca
    27241 agaaaatgct actgctggaa atactcggga taacgttgag taacagtttc ctgttctttc
    27301 agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt cttctggttt tttatcaact
    27361 aaagccttga gtaaatcata atgttgttcc atgatcgttt cccatggaag cccctgaatg
    27421 gtcactcgta accagcggaa acgttctaaa tgggtattat ggtcatctaa ccagttccaa
    27481 acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa
    27541 aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt cttctaaaat cgtttctagc
    27601 actattcgtg tttgtgtgtc caatttggaa caacattcaa tcatgatttg ctcttccaac
    27661 tgttctcgga caaagcgagc attttctgca gaaacaagat taattttgga aacatacgta
    27721 ccactttgag gtatcgtata cacgagttct tgctgacgta gttgaatcaa tgcttcgcga
    27781 atgggagtcc ggccgatagc aagatgttcc tccagtgcct tttcagatat tttccttcca
    27841 ggttctaagt cagcatagat aatttgttta cgaatcgctt gataagcttg tgattgcaag
    27901 ctgcttttca tctttattcc tccaaaatca tttctatgaa cgtagtataa cagaagaggt
    27961 ctgaaaaaca atctggtatg tcacattaac tagtaaattt gtataaatag taaaattttg
    28021 gttgacatag cgatttttat tgcataaaat tcacttgaat acaaaaacta atatgttagt
    28081 ttaggagggg aaaaatgatt gatttaaaca actggaggaa ctttatccct gaattatctc
    28141 aaaaagaagt agaaatacct cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa
    28201 atcctgtctg gatccatttc ggtggcggaa atctttatcg aggatttcat gcagaaatcg
    28261 ctcaacgttt agcggatcta ggcgaattgt ccactggtgt cattgtttgt gaaacattcg
    28321 atgaagaaat aattgaaagg gcttaccaag attttgatga tttgattctt gaagtggtca
    28381 tgccaagcag tggagagttc gtcaaacgta tattagctgc gacagctgac agtctgtatt
    28441 gtcatccaga ttgttctgaa cactttgata agatgattcg acactttgaa aatcctagtt
    28501 tacaattagt tacttttgct attactgaaa aaggttatcg attgaaagat acaaataata
    28561 aatatatagg accgattgca gtcgatataa aaaatggtcc aaagaagcca tcacatacaa
    28621 tgagtattct tgctgtgcta ctttataaac gttttttatc tggagcttgg ccgattgcac
    28681 ttgtctctac agataatttt tcacaaaacg gtagtcaatt acgtacttct ttgctaacga
    28741 tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac atttatccaa tacttatcga
    28801 atgaagaaat tgttgcgttt ccatggacga tgattgatcg tatcaccccg aatccttcct
    28861 tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg
    28921 caaaaggaaa taatattgct ccatttgtca atacagagga catccattat ttaatcatgg
    28981 aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc
    29041 gtgcaacggt agataaagca gatacgatga aggtgacgac ttgccttaat cctttgcata
    29101 cgtctttagc tattatgggt tgtttactgg ggtataggag catagctgag gagatgaaag
    29161 atccagattt atcgctgcta gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg
    29221 agtctccagg tatcattgat ccaaagatct ttctggaaga agtaattact gtgcgctttt
    29281 ctaatccaaa tattcctaat atgcctcagc gaatcgccac ggatacctct caaaaggtgg
    29341 cgattcgtta tggtgagacg attaaaaaat atatggaaat agaagttaaa gacccgcaga
    29401 cacttgtatt cataccattg actctcgctg cttggcttcg ataccttatt ggcattgatg
    29461 atcaaggaaa accattcatc ccgagtccag acccactatt gacagacctt cagcataagc
    29521 tttccgatat tcaactgggt acgcaagata catcacatat tcatgagaaa atggctccta
    29581 ttttgtcaaa taaagaaatt tttggtgtag atctttacca agcagagctt ggcagaaaaa
    29641 tagaagaaat gttcatcaag atggtagcag gaccaaatgc cgtacgaaaa acattacagg
    29701 attacttaca agaacatgga ggaaatttat catggaaatg acttttagat ggtacggatc
    29761 acaggaagaa aagattacct tagatgaaat cagacagatt cctgcggtca aagggattgt
    29821 aggcacattg atggatatac cagtaggaga ggtatggcct agggagagga tcaaagaact
    29881 aaaagaagaa attgagtctc gtgggttaac actaaaagta attgaaagcg tcaatgtgca
    29941 tgatgccatt aaaattggat taccttcccg tgagcagtat atcgaaaact ataagcagac
    30001 aatccgaaac ttggcagaat atggaataga agtcatctgc tataatttta tgcctgtatt
    30061 tgactgggtg aaatctgacc ttgactaccg cttagaagac ggctcatcca cgttagcttt
    30121 tatatctgat gatattccag atgatccaag agaaatcgtc gcgcgcatcg aacattccag
    30181 caatgaattt ggattgccgg gatgggaacc tgagcgctta gctcctatca aatccttgct
    30241 tgaagcctat gcatcagtag acgaagaaaa acttcgagaa aattttgcct attttctcca
    30301 atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc
    30361 gccatactcg atttttggat tagctcgaat cgtaaagaat cgagaagatt tagattggtt
    30421 gtgtcgcata gtagacagcc cttataacgg aatcactctt tgtacaggga gtattgcaga
    30481 agatccagaa aatgatgtat atgggatttt agcagaattt tgccaaagag atcgaatccc
    30541 atttgcacat gttcgaaata ttaaattttt agctaatcga gatttctatg aggcaccaca
    30601 taaatccgaa tacggttcat tagacatgta ccgcatacta aaaacaatgt atgacaacgg
    30661 atttaacgga tacatccgtc ctgatcatgg aagaatgatc tggaatgaga caggaagacc
    30721 aggatacggc ttatatgatc gagcattagg tgctgtttat ctaaatggat tatgggaagc
    30781 aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa aaactgtaat tttattatgt
    30841 aaataagaag gatgttcaaa ttcttgatac ataaatccgg aggttgttaa taagcattcg
    30901 gatttatgta tcttatttgg tctaggaagt aacttgattt tcttttttcg attgatccat
    30961 gagtgcttct tcttttaatt caaagatata ttctgagatc ctcataatca cttttttcca
    31021 gtcttctggt gtgatgattc cttttaaaat atagattttt ttgatatgag aaggcaattg
    31081 tttcaaaggg cagtcgctga taacgatgtt gcagttctcc ataaaatcat tttgtatttt
    31141 gatcgattca tttgaaaaaa tttgttctat cttaacgatc agatctttac tgatataaaa
    31201 atcacgcgta tagttcacac caatcacaat tcgcggctgt tttttgtagc gatcatacaa
    31261 atgtactagg tcttcaacca tccattgcac attttccttt tctaaaataa ctggtaacaa
    31321 gtgttgattt tgttctctga aatccatata aaattgttca atggcggttt ggacttcttt
    31381 caatacgcta tgttcttgat aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa
    31441 gttaagaaat ccattataaa gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc
    31501 tttttcaggt ataaacaaat caaatacagt acaaagggac tccaccattt ctcttgaata
    31561 aagaacatga ggaacgtttg ctttttttaa aagtttgtat tgctccaagc ttgtttcttc
    31621 cgattcgatt ttcgtgatag aaagtcgagc taaaatattc actaagatgc gttgttcgcg
    31681 acaaattggc agattaggtc gcaaaatatc tacagagaga tcaataaaaa agtcaatata
    31741 ttcatactct gtgaattctt tttgtacgtc tttcacagaa gttgcaggaa aattctcttt
    31801 acatattttt aatagaaaat ataatttatc tatcataccg ctatttagtc ggtcaagtac
    31861 ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt catcataaaa
    31921 ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat attgaatttg
    31981 tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag acaaaccgta
    32041 ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg cactatagtt
    32101 tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag aagtcaagat
    32161 ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc agtaagccgg
    32221 atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat gcagttctgt
    32281 tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa aatttgattc
    32341 agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat gttcagatag
    32401 gaaaagaaaa atatctaatt ttgtattgct ttttttcttc aagataaacg ttttaatcgc
    32461 ttcttgagtt tgcatgaaag aggctccttt ttacttaaat tgttaaaact tgaaaatatt
    32521 actgtttttt tcataaattt agacaaaaaa acagtaaaca tataggtaga atctctacta
    32581 tcttatttct tgcatacaaa aaaatcaagg agtgagagag ttatgagata ttcaaccatc
    32641 ttaatatcaa catttgcagc cttaagctta tcgcttacta taaatcagac agtccacaca
    32701 gaggagacag aaacagccaa atggacagaa accaagtggg ccaacgtcaa tcgaattacc
    32761 tttcaggatg aattttcatc cttcaattca ggagtcgcaa aattaagaaa aggacaaaca
    32821 gcgaccatca aagcaaaaat tgactattcg ggagacttag cacctatact acaatcagct
    32881 gttacatttg agaatgttga tcccactttg tcgattggct atgacgagaa cactttgact
    32941 atccaacaca agcaagcgat atttaccttc accgttacat taaatcagga cttgttaaaa
    33001 ccagcgcttt ttactgtcaa ggtatcagat ggactaccga acgatcatca tttaacgccg
    33061 tatagtcagc ggcagacaat tgagcaagat tcagccatcg actcaggagg agatcttgtt
    33121 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
    33181 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
    33241 ccagagccag acaataaatc aaaacctgaa ataacccctg gagaaaaacc agatgtaact
    33301 ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc agacacagat
    33361 aatgaaactg aaaatccaga aaaaccagag gtagatccag aagaaaaacc agatgtgact
    33421 ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa caaaaaaaca
    33481 attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct aaaaataaat
    33541 gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc tgaaacctta
    33601 aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat
    33661 ccggagagtc acaaaacaaa gttatactgt ggagcggaat cattctatta agtatagcaa
    33721 caatgctttc tgcaaaaaga tttaaacaaa acagatctct ctaacatttg aataatgtct
    33781 gtgaacgcat ttttcaaata aaaagaaata tccagaaaat gaccccaaaa taccagattg
    33841 aaatctgata tttggggtcg ttcatacttt atctttcttc atatttattt taagggatat
    33901 ctgtttcttt tggaagatgg ttcgggatct ttttcacttt caatacatct aaaaagaagg
    33961 taaagatcgg aatgccgaca atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta
    34021 agaccacaaa tgtataaaaa atcggcagtt ctgttttgct ggacatcaac ttcggattca
    34081 gcacataaga ctcaattaga tggacaatca aaatcgtaat caggatataa acaacatctc
    34141 ggatgcctcc ttgggaataa gctatgaaag atagaggaat acaagaaatg ataacacctg
    34201 ctactgggat cagacttaaa atgaaaatca tgatcgctaa actcagcagc tgatgaaagc
    34261 caaatacagc caaggcgatt gtagtgagca acgtatttaa aagagcgaca acaaattgag
    34321 cttctaatac caaaccaaat gtattgacaa acttatcggc aaaatagtaa atatcttgaa
    34381 agaaccaaga aaattcactt tttaaaaaca atcttgagaa tacaattgtt tttttctttt
    34441 caatcataaa aaagaaactt aagatgaatg acatagcaaa cgccactgcc atttttccta
    34501 tatcctgtaa atagccaagc gcaatcgaag cgccattttg aagctgttca aaaaaatcgt
    34561 ttcgctcaat ataagtatgg atatattgta aaacaggatc tgcatctttt ggctgatgct
    34621 cgtaaaaatt aataacggac tggatcatgt caaatgtttg atgaataagc atcgggatat
    34681 actttgtgaa tgccagatag ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa
    34741 cacctgtagg gattcggaca aatcgctgaa tgatttgaac aagatgaatc atcaaataag
    34801 taaagataaa tgtgagcaaa atggttgtaa tcatacttct aactaagaaa agaacaaaaa
    34861 cgacaaataa taaaacagtg agacggcgta ttttttcatt cgccaaaaaa cgctgataaa
    34921 cggtcaaatc gacaacctcc aaataacaaa aaataaacac gttattcatt atatgatgga
    34981 atatgaaaat aagaaagagc aaaaagaaaa accgctaaat gacgttatct tatgagttgg
    35041 ttttcctcat aaaatcgttc atctagcagt ttcagccctt tttctggtct tgatgaaaat
    35101 tatctagcat ttttcgggtc agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa
    35161 gataagactt gatcttttgt ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag
    35221 cagtcagcgt aacatttacg attctttcat cacccgtttc acgctgacga ttaatataat
    35281 ttttttctgc taactttttt agtaagggag tcagcgtacc agaatccaga cacaaatcat
    35341 ttcctagctc tttcactgaa gtctttttct tttcccataa aactaacatc acaagatact
    35401 gggtataggt aagattcagc ggcttgagga ggggagtgta gtgtctaatg atttctttag
    35461 aggcggcata taaaggaaaa catacttgat taactaaata tagatcttcc atttttccac
    35521 ctccgctgtt ttactatagt gtatcatagc ggatttacac aaaaaagtct attcactaac
    35581 tgctttcaga gtaacttcta tatttccagc tgtagctttt gaatacggac acactttatg
    35641 cgctgcctct aataattctt gcgtttcttc cagagaaaga ccttcgatag atccttcaat
    35701 ctctacacct aagacaaagc tcgtttcact ttctgaataa agggaaactt gtgcactgac
    35761 agtagaagcc ccagagatat ttttttgctg tttgatcaat tctaatgcac tgttaaaaca
    35821 tgcactgaaa ccagctgcaa acaattgttc agggttggtt gcattttcga cgcgtttacc
    35881 aggagctgca atattcaatt cgaatgtacg atcgggatta tgcacttctc cagagcgtcc
    35941 gcctgtgttg atcattttcg tagaatacat ttttttcata aaattaccac ctttaattat
    36001 attttataca attgaatttt acacaatata attgtggaaa gcaaagaaat ggattggcat
    36061 aagataaata agagaaagca catgaaacag acgcgctttc tcgaatagca aacatataca
    36121 aaaaatatca gagacaaaaa ataatctcat cataaacatt acatatattt ctcaaaaagt
    36181 tgttttcttt cttcaaaaat tacttcacga tggacctgat gaagcatcca taaataacca
    36241 aaaggatctc tgaacattga gttagtcaca ccaaagtcca tcatttctgt catttcttga
    36301 atctctacac agccatttcg taatgcttta ttatgtgtgt cctcgatatc agagacgatg
    36361 atattgaacc agatcgtatt tggatgttca gcagtaggag cagtcaaacc aagagacgtg
    36421 ttttcatcca gcatatggaa tctcgtacca tacaaggtaa aaatgacttc attattgcct
    36481 ctagggtaat ctgttgcctc aatcagttgt atttcatcaa agattgtttg atacagttct
    36541 agtgctttca aactatcatt taatacaaaa tcgatttccg ttcctttatg cataaagatc
    36601 tcctctcctt ttcagtcgaa cagttactag attcttttat tataccagct aaagaaaacg
    36661 cattcaaaaa aatagcggat ttacttacca actcattcga ttggatgata agtaaatccg
    36721 cgtcccatta cttctgctac gctgctgaca atgacaaagg cacttggatc gatttcttct
    36781 atcaatggtt tcattcggga aaattcatga tcagtcacaa cgatcaataa aacctctttt
    36841 gactttcgat tatacccgcc ttttgcctct agcagggtaa ggccacgtcc aatctcagca
    36901 cttaagcgaa ggcggatgtc ttccaaataa tgttcactca tgaccataac agatttcttt
    36961 cgattgatac ctgtttcgat ataagtcatg acaattgaag tgattgcaat agataaaata
    37021 gcataaagaa actcttctat tccaaaaata aacagattaa gagaaacaac gaatgcgtct
    37081 gtaaaaaata agccaatcga agtattcaag tggaaatatt ttttaaaaat gaggggaggg
    37141 atcgtcgtac cgccgctaga agagttgttt ttatataaaa tcgcaactcc taaagcaaaa
    37201 atcgcacttc caaaaatgac agacaacaac cgatctgaag taatcattgt ttctggaact
    37261 attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta
    37321 cctaaaaata gaagagctag taccaacata acgatattca acactaaaat aactatagct
    37381 cggtcaaagc ctaaagcagc ttccagtaat attcctagac cgctgacacc tccagcagca
    37441 atatgatgag gccctaaaaa catattgata gcgactccta atatgaagag ggcaaaaata
    37501 atcttaatgt atgtccatat aaattgtctc gtttctttca tttattcact tccttaattc
    37561 attaataata ctcttatctt attataactt acttttcacc ttaagttaac tttaattaat
    37621 aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata cttctacgcc
    37681 aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct taagataaat
    37741 tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta agtctatcta
    37801 tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat aacatttttg
    37861 aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta aaacacaagg
    37921 aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata tgcatttcta
    37981 tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat caaaaaagta
    38041 tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca aaaacaacga
    38101 ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga agtgacgagc
    38161 gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac ggactgtttt
    38221 tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc aaatttttat
    38281 tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa
    38341 acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc
    38401 gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt cgggggagat
    38461 ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa tggcatctat
    38521 ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga ttattatgag
    38581 gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga agcacataaa
    38641 agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca ttcaccacag
    38701 tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt ccacatccgt
    38761 tttttcccag tagattctta tcagatgact gacttaccag aaactgctga aaatatccca
    38821 tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa tccagaagtg
    38881 caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat tgatggatgg
    38941 cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag agaagcagtt
    39001 acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc tcaagcttgg
    39061 ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc gattaaagat
    39121 tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa tcatcagcaa
    39181 atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc tcatgatact
    39241 gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt gatggccttt
    39301 atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg gatgactggt
    39361 gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca agatctagag
    39421 ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa gattatttct
    39481 gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta tttcacacgg
    39541 aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc ttgggtagta
    39601 gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga cgggaaagca
    39661 gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt aacaacaaga
    39721 aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc acgttgaata
    39781 caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct
    39841 ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca gcagaagggg
    39901 aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa gattatataa
    39961 acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg
    40021 acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag ggacctgacg
    40081 ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat ctggcggaag
    40141 taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg acttatgatg
    40201 gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat aacaaagatt
    40261 tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa gacagtcgtt
    40321 ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg actgactttt
    40381 attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat gatggcacaa
    40441 atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct tatgcaacag
    40501 attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg
    40561 ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca tggcaagcgc
    40621 aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact ctaaataatg
    40681 gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa
    40741 acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat
    40801 tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca gcaaaggcta
    40861 aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa gcgatgccaa
    40921 acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc gatgcagcat
    40981 caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc agtgaagcca
    41041 tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat ggaacaggtg
    41101 cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt gccattatct
    41161 taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc aagataaaac
    41221 aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga tggggcagtt
    41281 tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc tattcattgg
    41341 tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg gaagtatacc
    41401 aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca tcattacaat
    41461 cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag cgaagatgcg
    41521 tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata taggtgatga
    41581 aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta cgatcatttt
    41641 cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc atattccacc
    41701 agccagttta attgattggg taggggctaa aaactttttt agtatcttct ttttaagctc
    41761 atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt tatgtgcaac
    41821 aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga aatttattaa
    41881 attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag cttttattac
    41941 tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata ctcaagtgat
    42001 tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga aaacggaccc
    42061 gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc cctatatcta
    42121 tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag cagcaaaaat
    42181 cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg tcttcctagt
    42241 ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct ccatgatcta
    42301 tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta ctgatattct
    42361 tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta tggcttcagc
    42421 aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac
    42481 aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc tcgattccaa
    42541 aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt cattatcatc
    42601 tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc agcttttagt
    42661 ttagatcttt caagtgactg gactttagga aactttactc ggctattcag tgaaacactt
    42721 tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat cgtacaagtg
    42781 actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg ccgcaaatca
    42841 agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt gactgccttt
    42901 tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac aatgatctat
    42961 ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga cactgtacca
    43021 atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat ttttttccag
    43081 attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc ttttatggga
    43141 ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt
    43201 gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc tctttttgct
    43261 gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt acaaaagaac
    43321 tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat catgcaaaca
    43381 tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc gggacgcaaa
    43441 ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt gatggtcatg
    43501 cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca atttctatcc
    43561 gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga attatctgaa
    43621 actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg
    43681 tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga gaaggaacaa
    43741 tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa ttatagccca
    43801 tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt agaaagagaa
    43861 ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct tggtcttgtc
    43921 ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg gatgacaaga
    43981 aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat gctgaatgtt
    44041 atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga ctttatcttg
    44101 atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca
    44161 agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg
    44221 aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa cttcttagtt
    44281 gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag gaaaatcatg
    44341 ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc tgtcgaagta
    44401 aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc ccaaatcaaa
    44461 atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcatc tttggcacta
    44521 ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc attgatcaac
    44581 gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa atggctgcca
    44641 aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca ttatgactta
    44701 ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg tgcttatttc
    44761 aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca tatcttagac
    44821 aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg gggaacattg
    44881 atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact aagtgaagaa
    44941 caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa atgccgtgta
    45001 ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac aagtgtaggt
    45061 atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt aaaagattta
    45121 ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg cggtgcgaca
    45181 aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt tactgaatta
    45241 gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac
    45301 taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga tcaagtcacg
    45361 aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc ttgtgcagct
    45421 tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc tggaaacaat
    45481 gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac tatttaaaaa
    45541 gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct aggattgtct
    45601 ctaaagctta gtttc
//
LOCUS       DAFFFQ010000014        42888 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000014
VERSION     DAFFFQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.014"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            6..305
                     /locus_tag="LQB69_000869"
     CDS             6..305
                     /locus_tag="LQB69_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3525051.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LQB69_000870"
     CDS             378..1739
                     /locus_tag="LQB69_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL3525052.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LQB69_000871"
     CDS             1761..2021
                     /locus_tag="LQB69_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525053.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LQB69_000872"
     CDS             2035..2787
                     /locus_tag="LQB69_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL3525054.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LQB69_000873"
     CDS             complement(2881..3486)
                     /locus_tag="LQB69_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3525055.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LQB69_000874"
     CDS             complement(3539..4513)
                     /locus_tag="LQB69_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL3525056.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LQB69_000875"
     CDS             complement(4891..5610)
                     /locus_tag="LQB69_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3525057.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LQB69_000876"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LQB69_000876"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL3525058.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LQB69_000877"
     CDS             7161..8558
                     /locus_tag="LQB69_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3525059.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LQB69_000878"
     CDS             8639..9079
                     /locus_tag="LQB69_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3525060.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LQB69_000879"
     CDS             9227..9553
                     /locus_tag="LQB69_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3525061.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LQB69_000880"
     CDS             9554..10303
                     /locus_tag="LQB69_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525062.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LQB69_000881"
     CDS             complement(10383..10838)
                     /locus_tag="LQB69_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL3525063.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LQB69_000882"
     CDS             complement(11265..11540)
                     /locus_tag="LQB69_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525064.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LQB69_000883"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LQB69_000883"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL3525065.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LQB69_000884"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LQB69_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL3525066.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LQB69_000885"
     CDS             14770..17436
                     /locus_tag="LQB69_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3525067.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LQB69_000886"
     CDS             17800..18552
                     /locus_tag="LQB69_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3525068.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LQB69_000887"
     CDS             complement(18574..19728)
                     /locus_tag="LQB69_000887"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL3525069.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LQB69_000888"
     CDS             19913..22354
                     /locus_tag="LQB69_000888"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL3525070.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LQB69_000889"
     CDS             complement(22684..23388)
                     /locus_tag="LQB69_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525071.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LQB69_000890"
     CDS             complement(23575..23820)
                     /locus_tag="LQB69_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525072.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LQB69_000891"
     CDS             complement(23837..24688)
                     /locus_tag="LQB69_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL3525073.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LQB69_000892"
     CDS             24854..25858
                     /locus_tag="LQB69_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL3525074.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(26099..26455)
                     /locus_tag="LQB69_000893"
     CDS             complement(26099..26455)
                     /locus_tag="LQB69_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3525075.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            26823..28010
                     /gene="tuf"
                     /locus_tag="LQB69_000894"
     CDS             26823..28010
                     /gene="tuf"
                     /locus_tag="LQB69_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL3525076.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LQB69_000895"
     CDS             complement(28198..30789)
                     /gene="mprF"
                     /locus_tag="LQB69_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL3525077.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(30815..31576)
                     /locus_tag="LQB69_000896"
     CDS             complement(30815..31576)
                     /locus_tag="LQB69_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3525078.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            31854..32375
                     /locus_tag="LQB69_000897"
     CDS             31854..32375
                     /locus_tag="LQB69_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL3525079.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            32434..33294
                     /locus_tag="LQB69_000898"
     CDS             32434..33294
                     /locus_tag="LQB69_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL3525080.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            33479..35794
                     /locus_tag="LQB69_000899"
     CDS             33479..35794
                     /locus_tag="LQB69_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3525081.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            35976..37817
                     /locus_tag="LQB69_000900"
     CDS             35976..37817
                     /locus_tag="LQB69_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL3525082.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            37841..38950
                     /gene="rpoD"
                     /locus_tag="LQB69_000901"
     CDS             37841..38950
                     /gene="rpoD"
                     /locus_tag="LQB69_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL3525083.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            39170..41269
                     /locus_tag="LQB69_000902"
     CDS             39170..41269
                     /locus_tag="LQB69_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL3525084.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            41465..42328
                     /locus_tag="LQB69_000903"
     CDS             41465..42328
                     /locus_tag="LQB69_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL3525085.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            42433..>42888
                     /locus_tag="LQB69_000904"
     CDS             42433..>42888
                     /locus_tag="LQB69_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3525086.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT        14098 a         7511 c         8868 g        12411 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtgag acaaaagtaa agaacacttt
    25981 tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt cttatttctt
    26041 ggagttaaac gcttctgtcc cagcctcttt ttatctaata aaattttaag accatttact
    26101 aattatcgat tttttctgcc gcatttttag gcacttcaga agcctttatt tcttcccagc
    26161 ttaatacacc tttacgagga ttgactttga gtttaagata cgcattcatc tttaatggat
    26221 gttctctttc ttcgcgaagc gtcacctgtt ttttttcacc atctttatta taagccgctt
    26281 gcttgtaggt ataagtcgtg aatttctctc cgttatctgc ttgggtagct gacatttcgc
    26341 cattcgtcgt gatttttgta taataagctt cgccgccgta aaaataatga taagctgcaa
    26401 aacttccgcc gagaaatatg cctaaaacga caagtacgcc aattaatttt ttcatgtact
    26461 tcacctcttt ccttattatt aacgaaatag aagatttcgt ctattgaaag ttgttggcaa
    26521 cagctgaatt ttaaactttt cctcattttg ttcttattgc ttcaaaagaa agttaaggac
    26581 tatcttgaag tttttctcaa aaaacacatt tttttattta taaaaggtac ttttttcgcg
    26641 tataagacag acgagtgctc ttagttgact ttgaccaata ttgacgtata ataagcttag
    26701 ttcgaatctt acaaaaagta agtcggacgt attctttttg atttagtcaa aaaaagacta
    26761 taataaaaag gtgagagctg aaatttttca gtgatctata ttatttagga ggtttgtaaa
    26821 ttatggcaaa agaacattat gacagaagta aaccacacgt taacattggt acgattggac
    26881 acgttgacca tgggaaaact actttaactg ctgcgatcac tacagtatta ggcaaaaaag
    26941 gcctagcaaa tcctcaagac tatgctagta ttgatgcagc tccagaagaa cgtgaacgtg
    27001 ggattacgat caataccgca cacgtagaat atgaaacaga aaaacgccac tacgcgcata
    27061 ttgatgcacc aggacacgcg gactatgtga aaaacatgat tacaggggca gcacaaatgg
    27121 atggtgcaat cttagttgtt tctgcaactg acggtccgat gcctcaaaca cgtgaacaca
    27181 ttttattgtc acgccaagtt ggtgtaaaat acctgattgt tttcttgaac aaagttgatt
    27241 tagtcgatga tgaagaattg atcgatttgg tagaaatgga agttcgcgag ttattgagcg
    27301 aatatggttt cccaggcgat gacactcctg tgatcaaagg ttccgcatta aaagcattgc
    27361 aaggcgatcc agatgctgaa gctgctatta tggaattgat ggatacagta gatgaatata
    27421 tcccaacacc agaacgtgat acagataaac cattactatt gccagtggaa gacgtcttct
    27481 caattacagg tcgaggaact gttgcctcag gtcgtattga tcgtggtgct gttcgtgtcg
    27541 gtgatgaggt agagatcgta gggatcaaac ctgaaacaca aaaagcagtt gtaacaggtg
    27601 tagaaatgtt ccgtaaaacg ttagattacg gggaagctgg ggataacgta ggcgtgttgt
    27661 tacgggggat ccaacgtgac gatatcgaac gtggacaagt acttgctaaa ccaggttcca
    27721 ttactccaca tacaaaattc aaagcagaag tgtacgtgtt gacaaaagaa gaaggtggac
    27781 gtcatactcc attcttcaac aactatcgtc cacagttcta cttccgcaca actgatgtta
    27841 caggaacaat cacattgcca gaagatacag aaatggtcat gcctggtgac aacgtgacga
    27901 ttgatgttga cttgatccat ccaatcgctg ttgaaaacgg aacgacattc tcaattcgtg
    27961 aaggcggacg gacagtaggt tcaggtatcg ttacagagat cgaagcataa gaaacgcgac
    28021 tcgaatattt tggtaatgaa aaatgtttaa aataaaagag cggtccaaac aggatttctg
    28081 tttggaccgc tctttgttct tcaattgtat aagaagagat tgtagaagca tgaactttcg
    28141 ttttacttca gtctctttta ttcatcatga gagtgttcat ctatcggtat gcagttttta
    28201 atactttctt cgtatccatt ttttcaaacc acttattcta ccttgtgtct gctcaactgg
    28261 cgcattatca ataatcagca aagcaatcat aacatatgca atccaactat ctctagaata
    28321 tagagtataa cgtgagaccc agtttgtagc gtacttgctt ttatattctc ttaatccatg
    28381 gaaagaataa aaatgagaac caaactcgta aaccaagtat gcaatccttt cctgtaaaaa
    28441 actctttctg gaagttccga catttgaaag aggggccatc cctaaattga accattggat
    28501 atgttcttct ttcatatagg caaacaaatg caagaacaag aaatccatac tgccagaagg
    28561 tgctttttta gaatcatatc gcatcagatc aatcgttcct actttattgt tcgtgtaagt
    28621 aggcatgatc gttgcaaaag cgacaatttc ttcttgggta tttttgacaa ctgcaatagg
    28681 cgctctttgt agataatctt cagagaaaaa gcctaaagaa aacccttttt cctttcgtga
    28741 acccaaccaa ttatcagaga tatttttgaa agtactcatt tgctcagtag aaaatggtgg
    28801 ttgtaagaca tcaaatgtaa atccttctcg ttcgatccga ttcaagacag cacgagtgcc
    28861 cttcattttt ttccctgaag ttgtaaaact attcaggtct acataagctt cttcccccat
    28921 cttaatgaaa tcgtagccga attcatgcaa aatcataaca atctcttcgc ttgtttcgta
    28981 gaaaactggt aaatagcata gtcgatctgt ttcttcgata aaagcttcga ttgcttctgg
    29041 aaaatcttct ttcttaccag aaggatcacc cattacaata catttattat tgaagcaagc
    29101 gaattggaga agaacagttg gttcaccgtc tttttcatag gcaaacatcc gcttatcttt
    29161 taagaagatc aattgactat cggagtttcc tccataagtt gtaaggatct tcagggcctt
    29221 ttcttcatta aaggcttcgc caatctgttt cttttctcct tgaaggaaat gaacaaatag
    29281 aacgatcatg aaactaacag cgatgatggc caacaatccc gaaaaccaaa tcttttcaga
    29341 aggaaataag aaaaaggaaa taaaatggtg acgccgatga gggaaatccg gcagattgta
    29401 taccccaata acgatgtaaa gcaaggacag tgcaccaata atgattccat cgatcgtacg
    29461 ccactcccat gaatagatca attgttcccg aaataattcg tttttagaag caagaatgat
    29521 cagaagcaga atagacaaga aaaccaccgg tgtgaaacta aaatcactta ataaaacata
    29581 aagtaaagct agaacaatca agaagattgt aggcaaatat gctcgtttca cacgtgctgc
    29641 gatccctctg cccataatga ttaaaagaaa tcccaatagt atactaggaa attggataat
    29701 aaaatgaaat ttcaatgggt tcaaactatg aagccagcga aactctgtaa aagcttgcgg
    29761 gatagtggca gaaagtacta gcatgattcc agaaaagtat aataagacaa ccacgatttt
    29821 atgcgcaatt tctgttgcta attgtttagg gataccagaa taacgctgat caaaagtgga
    29881 accgatattt ttgaagaaaa agacgattcc aatcaaaaac ggaatgatgt agtagaacag
    29941 gcgatagagc aagatccaca caacgactat ttctcttggc acaccaatag cggacaaccc
    30001 gataatcatc attacatcga aactcccaat ttctcctgga atcatagaca cgattcctat
    30061 cactgaagca gcaatgaata atggaattgt ttgccacaga tcaatccgga tttccattaa
    30121 ataaccaaca gaaaggaaac taaacaggac accggtccat tccaacaatg aaactagcaa
    30181 tagctgtcct tgagttttaa gtgaggtacc accaaggaat cctttctttt tgaaagtagt
    30241 aaataaaaag acgacaggaa aatatagacc gccgccgatc aaccatatcc aatattgctt
    30301 caaaaaagga gcaactggcg tgaagactac aagtaaaaaa gaaagcagac tgtaaataga
    30361 aagtccagcc atcaaaaaca agaaaacttt cgataatgct tggatcacgt ttctgctctc
    30421 tttcttttgt ccatatagtt cagagcgaag ccccacacta acaaacccgc cgaaaccggc
    30481 aatattgttg atcgtattga tcagccagct tgtctcaaac aggtaacgag gattctgctt
    30541 ttgttcaagc atccgattca aaataatatc ataaccaacc ataggtaaca cagagataag
    30601 accaatcaat agcattagtc cgcttttcca tacaggtata gtggcaaacg tttctcctaa
    30661 ttgctggata gatagagttt taccgatgga catcaattca ccgacaataa tgacaatgac
    30721 agaaatcaaa aagattgttt tgaacagtcc taggtggttt ttcagccatt gtatggtatt
    30781 ttttaacaaa taaatcacct tctattccta atagttatgt ttcagaaaga aattgaagaa
    30841 cttctcgatt gaagcgttca ggatcttttc tagctaattc atgtccttgt tctttcacaa
    30901 gcacaaagga agcttttggt atagttttag ctatatacaa agaatgtttt agcttgatta
    30961 catctttttt tcccacgatg atcaaagtgg gggagttgat tttttttaaa tcattttctg
    31021 taagcccgat gtcatgcagc aaaagtttga taactaaaag atttttgcga agacttgggc
    31081 gaaataaaga gagtaaccat acccatgcat aatgaatatt actgatgact ctggcagaaa
    31141 aacgaacgcc tttaacaagc gtgtttccag aatttaaaat caatcgatga acttttttag
    31201 ggaagctgct ggcaaatacc agtgctagat ttgctccatc actaaatcca agaaaatctg
    31261 cctgatcgat tttttccagc aacatgatcg tattcagatc ctcggccatt aactgaaaat
    31321 tcaacataga tgcttcgttt gtcgaacgac catgtcctcg gctatctact agatacacgg
    31381 tatagtagcg ttcgagtaca gggacctgtt ctgaaaaaaa gcggccgctg ccgtcattgc
    31441 catgcagtaa aaagagaggg aagccttgac cgctcttttc atagtaaatt tcgctcccat
    31501 ctggcatttt tgcatatttc ttttcttttt tcacataatc acctactgtg taaagtataa
    31561 cgattatatc agtcattggg caattgtttt tactgaaaaa caacgatcat tttcgtaaga
    31621 tgtcaaaaaa tacaggaaaa ttcatttttt cttaaggcgt acacagaaat caaggaagaa
    31681 atagccaaga agataaagaa atagtaagat aaaaaaagat gtcaaaataa ctaggtgatg
    31741 aaatgaagcg aaaacgatta tctattatcc gtattctttt ttacatgatc attctgacgg
    31801 ttattggtgc ggtgttgttt ttatgaggaa gaacttatcg ttcggtaaaa cgagtggaaa
    31861 gctatcaaat agaaataaca acagctgcaa aaaattatca tatggaaaac tacgagaatt
    31921 tgatcaaggc gataatattg accgaatcaa aaggaaaagg gacagatctc atgcagagct
    31981 cggaaagtct gcatggagaa gctgagatca ttgataatcc agctgacagt attgatcaag
    32041 gtgtcaaata tttggcggaa atgatagtag aagcaaagaa acaaggatgc gatttatgga
    32101 cagctgtcca agcatataat tttgggaaag attatattgc ctatgtaaaa aatcatggcg
    32161 gagaaaacac agtagaatta gcagaagcgt attctaaaga tgtgctgagc ccacttttag
    32221 gaaatgagga agggaaacaa taccgatatt ggcggatcca gtccttgctt tacaatggcg
    32281 gctatctcta tcataatgga ggtaacatgt tttatcctga tattgtcaaa atgaatgaaa
    32341 aaataataga gacctatgaa aaagtcttta agtaaaatag caggaaatgt tcaatcatga
    32401 ataaaattaa atgaaagaag gagtgcgcaa atagtggaaa aaaggcaaaa aatggaaaat
    32461 caagaagtgg ggtacacaac caaagactgg atgttacggt ttgtgaaggg aatgtttatc
    32521 ggttcaggat ttatcttgcc aggagtcagc ggtggtgcat tagcagctgt ctttggcatg
    32581 tatgagcggc tgatttcttt tttagcccac cttacaaaaa atttcaaaga aaatgttttg
    32641 ttttttctgc ctgttggtct aggcggagtg acaggcatct ttctactttc ttttttagtc
    32701 agttttttat taggtgcgta tgaaacaacg atattatggt tttttgtcgg atgtatcgct
    32761 ggaactgttc ctgcgttatg gaaagaagca gggaaaaaag gaagagatac ttctgatgtg
    32821 atcatcatgg ttgttacttt tgttctggct tatttgtttt tattatacgg cgctcaatta
    32881 tttgacgggc aagtcgatca gaattttgga acatggctga ttgccggcgg attgattggt
    32941 ttgggtatga tcgtcccagg attgagccct tctaattttt tggtgtatat gggattgtat
    33001 aaagcgatgg ctgacggatt taaagaagta cgacttgaag tgattgtgcc gatcggaatt
    33061 ggtgggattc tttgtgttct tggattgtca aaaatcatgg attacatttt ctcaaaggca
    33121 tatagcaagc tattccattt cattttaggt gtagtttttg cttcaacgat tatgatcatt
    33181 cctaccgatt acgcgggatt gggagttgct ggggtactag cttgtcctgt tctttttata
    33241 gccggtacag cactaggttg gtggatgagc cgtttggagg aacaatataa gtaaagatag
    33301 attgataata aaaacagcac acgggtgatt caaacaaaaa ggttggcatc ttttttgtaa
    33361 aatcattcgt gtgtttttct ataaatttta taaaaacaag ttgtttttaa tcttggaaca
    33421 cgctagactt aaacacgtga ttatttttga aagttggcgg aaaatggaga aaaagaaaat
    33481 gaatggactt acacagcaag aggtagttca gaaaatggct tctggccagc aaaatgacta
    33541 ccaggaagat gcatcaaaaa gtacaaaaca aatattcagc gataatatat tgacgttatt
    33601 caattttctg aactttggta ttgggatttg tctgttattt gtaggtgcgt attccaacat
    33661 ggcgtattta gcaattattg tcgtcaatat cgttattgga atttatcaag aaatacatgc
    33721 aagaaatctt gtacggaaat tatcgattgt ttcccaatca gaagtactag tgatccggga
    33781 cggtaaagaa caatcgattt ctactaaaga gctggtatta ggagatttgg taaaaatcgg
    33841 agcaggagaa cagataccag ccgatttaca ggttatatct ggacgggcag aagcaaatga
    33901 agctcttttg acaggcgaat ctgatttgat tgtcaaagaa actggagctg aattgctttc
    33961 tggaagcttt ttgacaagcg gttctgtcat ggctgaagtt atccgagtag gtgcagataa
    34021 ttatgctgtc cgcttgacta gcgaggcaaa gactcacaaa gctattcgtt ctgaacttgt
    34081 agattcgatt cgcaaagtat ccaaattcac tagtttcgtg attattcctt tggggatcat
    34141 actatttttg gaagcactgt tcatccgcga tgcttctatc aaaatttctg ttattgcatc
    34201 tgcagcagct ttgttaggca tgcttccaaa gggtttggta ttgctaataa gtattgcatt
    34261 aacgacaggg gtaaccaaac tggcaaaaaa acgaatcctt gttcaagaca tgtattcaat
    34321 tgaaacgctg gcccatgtcg atacattatg cttggataaa acagggacca ttactgaagg
    34381 gaaaatgagt atccagcggg tagaagcact acatccgctg tacgagaaag ccatcccaga
    34441 aattatgggg acgtatctgg cagaaagcag tgataacaac atcactatgc gggctatgag
    34501 agattatttt gagctttcta atcggtatga tgtgacaaac aaagtcgcat tttcttctga
    34561 tcgcaaatgg ggcgcgatgg aatttccaga actgggagtc gtgtacgtag gagcgcctga
    34621 acgtttggtg gaagaagatc ggcttccgga cagtctaaaa acagcccaag cgaatggcta
    34681 tcgcgtactt atgttgggga tcgctccaaa tcagcaattg aacgaagaaa aaccagcatt
    34741 tgttgagccg gttgctctgt ttgaaatcga tgatccaatc cgaaaaaacg ctcaagaaac
    34801 actagcttat cttcatgagg aaggtgtgga tctgaaagtg atttctggag acaatccttt
    34861 tactgtctcc aatatcgcta gacgtgcagg attaccgaat tatgatgcgt atgtcgatct
    34921 ttctcaaatt actgaagaaa tggaagtgcg agaagctgct catcgctata cggtttttgg
    34981 acgagtgagt ccgcagcaaa agaaattgct ggtaaatgaa ttgaaagaaa gcggcagaac
    35041 ggttgcaatg acaggcgacg gtgtgaatga tgttttggcg ttaagagaag cagattgcag
    35101 cattgctatg gcagaaggtg atggagcaac acgtcagatt gcgaatctgg tattgttgga
    35161 ctctgatttt actacattgc cagaagtcct gtttgaagga cgccgagtgg tcaacaatgt
    35221 gacgaaagtc tcaggcatct tttttatcaa aacgatttat tcatttattt tatcgattat
    35281 atgtgccgtt acagcaattg gatttccgtt tatccctatc caaatcacgc tgattgattt
    35341 agcgattgaa gggtatcctt ccttcttttt atcttttgaa caagataaaa gaaaaatcac
    35401 ttaccgttat ttgcctacag cacttattaa tgctttacca aatgccttgc ttgtagtagt
    35461 gaatatcatt gcggtgtatc tacttggaaa aacattcgca tggggaaatg ctgaaactac
    35521 tactttgatg tattacttgc tgattgggat cagttgcatg gcagttgtaa aagcttgtta
    35581 tcccttcaac ccattgcgca tcttcctagc agtaacgacg atcgtaggga tatatgtagc
    35641 tgcaatgctg ttccatggaa ttttggaagt aaacttaggg ttgggagaga gcgtaggata
    35701 cttctttgta ttcatgctcg taaatattgt tcttcgagta ttgtactgga aggctgatat
    35761 tcaaaatcag ttactgaaaa gagtagcaaa ttgatgctta atgagataga aatgtgttat
    35821 acttgaaaaa gcaatgaaat atgatacaat caaacatgta taattgtgtc ctttaaaatc
    35881 gcactgattt cagttagtgc gattttttac cggttattct cctgaaatat cttctgttaa
    35941 acccaaaaga tatcaataga gcagggggtg aaaaaatggc tcaacggatt cctcaagagg
    36001 ttatcgaaac agtacgcagt caaacgaata tcgttgaggt gattggacaa tatgtccagt
    36061 tgaaaaagtc tggtaaaaat tacttggggc tgtgtccttt tcatgaagaa cgtacaccgt
    36121 ctttttctgt tgcagaagac aagcagattt ttcattgctt tggttgtggt aaaggcggaa
    36181 acgtgtttac ttttcttcaa gaactagaag gtctaagttt tccagaagca gtggtaaaag
    36241 tggcagactt cgaacagata ccgttagaag atcaatggcg tcagcgtact atagctgtcc
    36301 aaaatcctga atctgctacg gggaaactga ttgctgctca tgaaaaagca gcggaaatct
    36361 atcatcacat gctattgcat acaaaagctg gacagccagc tttagaatat ttgcaaagtc
    36421 gtgggttgtc tttagaatta attgaagaat tcaacatagg ttttgcacct aatgagcgtt
    36481 cttttcttca gcaagtattc aagaatgaat caatttcaaa tgaattgtta gaacgctctg
    36541 gattgtttgt cacgcatgat gatggccgta tgtctgatcg tttttatcaa agagtgatgt
    36601 ttccacttcg taattctcaa ggccagacga tcggtttttc aggacgctgg cttgcacctg
    36661 aaaaagggca ggaaaaagat cagcccaaat acttgaatag tccagaaaca gaactgttca
    36721 ataaaagaca agttttattt aatcttgata aagcaagaag cgatatccgg aaaaacggag
    36781 aaattttgtt gttcgaaggg tttatggatg tgattgctgc atggcaggct ggtatcaaaa
    36841 ctggattagc ttctatgggg acaagtctaa cagctcaaca gatccaacaa atggaaaagc
    36901 tgacgaaaga attggtcttt tgctatgacg gagacaacgc aggttttgaa gcaaccaatc
    36961 gagggattga attgcttcgg caaaacagtc gattagcact ttctgtcgta gtcgttccag
    37021 aaaagcttga tcctgatgaa tatctccgta agtatggaga agcatctttc caagaattag
    37081 tggctcatgg gcgcgaaaca gtctttactt ttaaatcacg ttatttaaaa caagggaaaa
    37141 atttagataa tgaaaaagac aagatcgaat acctcgaaga attactagtt gaacttagcc
    37201 gagttgtttc accaatcgaa caagatatgt atctatctca actgtcaaca gagtttcaga
    37261 tcggcagaga gacgatacaa aaacaactgc gcgatcttcg aaggacaaat cagcaaaatc
    37321 gtcaagaacc tcaggaaagt caacacactc aggttacgag acaagaaact gcacggaaaa
    37381 agagaccttt gacgcaagta gaaaaggcag agcagattct attatatcgt gcatttaaag
    37441 aagtttctgt ccgtaatatt ttaaaagaac gagaatttca gtttattcat gatgcatatg
    37501 cagaagttta ttttttattc gatgcatatg tctcacaaca ttatgaattc aaccttgcag
    37561 agtttttaga ctttttgcaa gatgaaaatt taaggcggtt ggtggtggaa atcgaatatt
    37621 taccggtggc agaagaaagt tcgccacgag aaatccaaga cttgctagat gtgatcagca
    37681 aatctggctt agctgatgaa ataacattaa aaaaacaaat gcagcaagaa gccagacgaa
    37741 ctggaaacaa acagcttgag cttgaattga cgattgaaat catcaattta acgaaacagt
    37801 taaaacaagc caaatagcta ctcaaggggg gccttctttt atggcaaatg gaacagataa
    37861 aaaaacgtat gaagcagcag tagcagcttt catcaaagaa aacaaaccca aacgacaagt
    37921 tgtatatgat gatttatcca atcagcttgc tacaccattc actttgaatg cagatgaaat
    37981 ggacaaattg atccaaaaga ttgaagacgc aggtatcagt gtggtggatg aaaatggtga
    38041 tccatcaatt cacagcttga agagtgctga gaaaaaagct gaacaagcaa aaacagaaga
    38101 tctttctgcg ccaaccggtg taaaaatcaa tgacccggtt cgtatgtatc tgaaagaaat
    38161 tggccgtgtc tcccttttaa ctgcggaaga agaagtagcg ttagcattga aaatcgaaga
    38221 aggcgaccaa gaagcgaaac agcgtctggc agaagcaaac cttcgtttag tagtaagtat
    38281 cgctaaacgt tatgtaggac gaggcatgca gttccttgac ttgatccaag aaggaaacat
    38341 gggactgatg aaggcagttg aaaaatttga ctaccgcaaa ggatttaagt tttcaaccta
    38401 tgctacatgg tggattcgtc aagccatcac tcgagcaatc gctgaccaag cacgtacgat
    38461 ccgtattcca gttcatatgg ttgaaacaat taataaattg atacgtatcc aacgtcaatt
    38521 gctgcaagac cttggaagag aacctacgcc agaagaaatc ggtgcggaaa tggatcttcc
    38581 aacagaaaaa gtccgagaaa tcttgaaaat cgcgcaagaa cctgtttccc ttgaaacgcc
    38641 aatcggtgaa gaagatgact ctcacttagg tgatttcata gaagaccagg aagcaacaag
    38701 tccagcagaa cacgcagctt atgagttact aaaagaacaa ttggaagatg tactagatac
    38761 attgactgat cgggaagaga atgtccttcg cttacgtttt ggtttagatg atggccggac
    38821 gagaacattg gaagaagtag ggaaagtatt tggtgttact cgggaacgta ttcgtcagat
    38881 cgaggctaaa gcattaagaa aattgcgcca tccttctcgt tcaaaacaat taaaagattt
    38941 cttagaataa gcgtggacta ttgaccagaa gagaaatcag taaaactgtt tggtttattt
    39001 taaaagaaaa aaagcttgag actacgcagt ctcaagcttt tttttctata ggaaggaaat
    39061 tcattcatta tttttaagta aatcctaaaa ataagctatt ggcgtgacgt atagaatatg
    39121 atagattaaa tggtagcgta aacctagtaa aggagccgat cgctaagtga tgaaaaaatg
    39181 tccaaattgc ggatcgaaaa aaatggagca gaatactatc tgtccagact gcggtcatca
    39241 aataactgca gaagatcagc aaataaaaga atcaattgaa gaaacaaata cagatgcagc
    39301 tatgcaggat aaatcaactg aagatgcagt ctttcctgat acagaattaa acgatccaat
    39361 cgaatggtcg gaattgaaag atttacctct agaatctgta atggaattat tcgaaaaagc
    39421 agaaccagaa gacgaagaac cacctgctca cgatcaagta actaatatag agaaaaatga
    39481 acagaagaaa gcaactgttc atgagacttc ggaagaatca ggaaagaatc aagaagaaac
    39541 gcttgaatca aaacaaaaaa gaagagtagc tgaactgaaa gaaatcgtcg ataatgagga
    39601 agaaaattcg atactatccg cttatatcaa agcacatcgt gaagatacaa aagaagagca
    39661 cgcgaaagag ctgctcaaaa tgatcagtga aaaaattgca caggaaaaca aagtagagcc
    39721 agaaaaggaa caagaaactg aaaaagactc tgatagagaa aacgccacag gtaaagaaac
    39781 agtaaaaata aaagacgtaa aaacggatat cacagaaaaa actgaagtat ttactgaaaa
    39841 acaattagaa aaggaaaaaa cgaataaact gccggaagca gagaaaaaag aaatactcga
    39901 aaacacagac aaaaagtcaa cagagcccga tgaaataaat agtgaatccg atgctgaaaa
    39961 tcaatctgta agcaaagagg gtactgatct tgaagcaaaa atagaaaaag aagagaacca
    40021 gcctaagctg tttgaagcag aagcgaaatc aataacaatc gtgccagata ctgaaaaaac
    40081 acatcaagaa tcaaacttag aaaaaacaga tccagataaa gcgcaaaaga aaaaatcaaa
    40141 aaaagtacct tatctcgttt tagcagcagt acttctactc ggtggaggaa gctgggctta
    40201 ttatgaccat catcaaaaag tagaagccga agtagcagca gaagcaaaaa gacagcaaca
    40261 aatggaagaa cttcatgaga aacttgctgc cttttataca gatgatcacc atcaatttat
    40321 tcgtgcagat atgattcatc aagatttatc aaaactaaaa aatgatttat ccagtcttaa
    40381 gaatgaaaaa ggatattcag agttagaaac aacttaccaa gatatccaat ctaagatccg
    40441 agatatccaa aaagtgaatg agtggttcat agcacctgcc atcgtcgatg atcatctagc
    40501 agatgacatc aagttgaaag cagataaaga gatccaagca atcgaaacgg ataacacaga
    40561 ttttggaaaa ttgattcaac aagcacaaac agaagctaaa aatcaatatg ctgaattgca
    40621 aacagctaaa gaaaaaacag ctatagtctt taaagacgga aaagtagtgg attcagcaac
    40681 acgcgaccaa tacaagacgg caaaagaagc agtagaaaaa gtgaaaaata cagatctatc
    40741 tgctcagttg aatgaacaat tgaaacaagt agataagatg ctcactgata aagagaaaaa
    40801 agaagaagag gaaaagaaaa aagcagaaga agcgaaaaaa gcagctgaag aacaagcaaa
    40861 acaagccgct caagcagcag ctgcagctca agccgctcaa caagctgcgg tcgcacaagc
    40921 agcacagcaa gaagcggcat cctcgacccc ttcctcttca aactcagcga atcagccaat
    40981 caaatctgcc agagcaagtg atattgccga tgcatctaat ccagcatgga cgtgggcacc
    41041 aggtgtccaa agcaaagtga ttgctacatg tatccaacgt ggatatatcg ttgaaggagg
    41101 ttacagatta gaaaaagcca gaatcgaaaa tggggaaggc tattataatc tatatgccac
    41161 ttcaacaaaa tctgcattga tgaaaggcat tggagaaagt gctttgccat tttacatcgt
    41221 aaccatcaac tgtaaaactg gatggttcgg aggaaatggg agcaattaat tttaggcaaa
    41281 tgaaagaggc tgtgagcctg ccgtttaaca ccgtttattc gaaaaagggg agtatgacac
    41341 aactgttgtc atactccttt tttcaccttt ccaaaccaag gctcagctat tcaagtagat
    41401 aacattatgt tatgatagca aagaaatgta ttaagaaaac ctttcactat agagggagaa
    41461 aaaaatgaac gatttattag caactatatt tacaggtatt attattgatg aaaatgaaaa
    41521 aaactacttt gtccaaaaaa acggacaaac ctttggctta agcaaatcag aaggtgaaca
    41581 caaaatcggt gaagcagttg aaggattcgg ttaccaaaat caaaaacatg aaaatcggat
    41641 cactacacaa attcctaaaa gccgtatcgg tcattacgct tttggaatcg ttactgctac
    41701 acgaaaagat ttaggtgcgt ttgtcgatat cggtcttcca gataaagact ttgtcgtatc
    41761 tcttgatgaa ttgccgacaa tgacagagct atggcctaaa aaaggggatc gcttgatgat
    41821 ttcccttaga gttgatgcaa aagaccgtat ttggggcagt ttagcagatg aaaaaatatt
    41881 caaatcttta agcaaatatg gaacagaaga aatgaaaaac aaagatgtga taggaacagc
    41941 ataccgcttg aaattaacag gtacgtacgt tttaacggaa gatttctatc ttgggtttat
    42001 ccatccttct gaacgatata atgaacctcg tctaggcgag cagataaaag gtcgagtgat
    42061 tggtgtccga ccagacggtg tactgaatct ttcattgaag ccaagagcat atgaagctat
    42121 atcagatgat gcacaaatga ttttaaccat tttagaacgt tctgcagatc agcaaatcaa
    42181 ctttacagat aaatcggatc cagaagaaat catgaaagct tttggtatca gcaaaggagc
    42241 atttaagcgc gcattaggta atcttttgaa acaaggattg attacacaag aaaacggtgt
    42301 gacaaaacta gtcaaaaaat ctaattgaga atcccttgca tgtttttctt aactagctta
    42361 taataatttt aattaacata gtgttatatc tttttttatt tgtaattatt ataaattgtt
    42421 ggggttgagt aaatggtcat gaatgctatt tcggcgatga aaaaaacaaa acagcagcta
    42481 catgaagcgg ggtttaagct gacaccgcaa agagaagcta ctctgcttgt gcttttggaa
    42541 aatgagaaag agcatttatc tgcagaagaa atcttctttt tagtgaaaaa aaagagtccg
    42601 gaaattggac ttgctactgt ttatcgaacc cttgaaatct tgacagaatt gaaaattgtc
    42661 gataaagtaa gttttaatga tggattagcc cgttatgatt tgagaaaaga aggcgccaaa
    42721 catttccatc atcatttact atgcttgaac tgcggcacga tcgaggaagt agaagaggat
    42781 cttttacttg aagtggagca agtagtagaa gaacgttatc attttttagt aaaagatcat
    42841 cggctaacgt tccatggaat ctgtcaggaa tgctatgaaa aagaaaaa
//
LOCUS       DAFFFQ010000015        37691 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000015
VERSION     DAFFFQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.015"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            163..1230
                     /gene="rlmN"
                     /locus_tag="LQB69_000905"
     CDS             163..1230
                     /gene="rlmN"
                     /locus_tag="LQB69_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL3525087.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1423..2526)
                     /locus_tag="LQB69_000906"
     CDS             complement(1423..2526)
                     /locus_tag="LQB69_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3525088.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3079..3627
                     /gene="aac(6')"
                     /locus_tag="LQB69_000907"
     CDS             3079..3627
                     /gene="aac(6')"
                     /locus_tag="LQB69_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL3525089.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            4173..5834
                     /locus_tag="LQB69_000908"
     CDS             4173..5834
                     /locus_tag="LQB69_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3525090.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(5904..6251)
                     /locus_tag="LQB69_000909"
     CDS             complement(5904..6251)
                     /locus_tag="LQB69_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL3525091.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            6335..7276
                     /locus_tag="LQB69_000910"
     CDS             6335..7276
                     /locus_tag="LQB69_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525092.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            7277..8320
                     /locus_tag="LQB69_000911"
     CDS             7277..8320
                     /locus_tag="LQB69_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525093.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            8326..9396
                     /locus_tag="LQB69_000912"
     CDS             8326..9396
                     /locus_tag="LQB69_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525094.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            9386..10339
                     /locus_tag="LQB69_000913"
     CDS             9386..10339
                     /locus_tag="LQB69_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525095.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            10351..11361
                     /locus_tag="LQB69_000914"
     CDS             10351..11361
                     /locus_tag="LQB69_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3525096.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(11646..12467)
                     /locus_tag="LQB69_000915"
     CDS             complement(11646..12467)
                     /locus_tag="LQB69_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL3525097.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(12564..12842)
                     /locus_tag="LQB69_000916"
     CDS             complement(12564..12842)
                     /locus_tag="LQB69_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525098.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            13110..15119
                     /gene="metG"
                     /locus_tag="LQB69_000917"
     CDS             13110..15119
                     /gene="metG"
                     /locus_tag="LQB69_000917"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL3525099.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(15598..16185)
                     /locus_tag="LQB69_000918"
     CDS             complement(15598..16185)
                     /locus_tag="LQB69_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525100.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            16360..17682
                     /locus_tag="LQB69_000919"
     CDS             16360..17682
                     /locus_tag="LQB69_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3525101.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            17837..18613
                     /locus_tag="LQB69_000920"
     CDS             17837..18613
                     /locus_tag="LQB69_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL3525102.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            18606..19175
                     /gene="rnmV"
                     /locus_tag="LQB69_000921"
     CDS             18606..19175
                     /gene="rnmV"
                     /locus_tag="LQB69_000921"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL3525103.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            19168..20052
                     /gene="rsmA"
                     /locus_tag="LQB69_000922"
     CDS             19168..20052
                     /gene="rsmA"
                     /locus_tag="LQB69_000922"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL3525104.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            20503..21132
                     /locus_tag="LQB69_000923"
     CDS             20503..21132
                     /locus_tag="LQB69_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525105.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(21256..22365)
                     /gene="ugpC"
                     /locus_tag="LQB69_000924"
     CDS             complement(21256..22365)
                     /gene="ugpC"
                     /locus_tag="LQB69_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL3525106.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            22574..22996
                     /gene="mgsA"
                     /locus_tag="LQB69_000925"
     CDS             22574..22996
                     /gene="mgsA"
                     /locus_tag="LQB69_000925"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL3525107.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            23319..23855
                     /locus_tag="LQB69_000926"
     CDS             23319..23855
                     /locus_tag="LQB69_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL3525108.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(23895..25658)
                     /locus_tag="LQB69_000927"
     CDS             complement(23895..25658)
                     /locus_tag="LQB69_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525109.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(25661..27376)
                     /locus_tag="LQB69_000928"
     CDS             complement(25661..27376)
                     /locus_tag="LQB69_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525110.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(28155..28379)
                     /locus_tag="LQB69_000929"
     CDS             complement(28155..28379)
                     /locus_tag="LQB69_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525111.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            28570..31347
                     /locus_tag="LQB69_000930"
     CDS             28570..31347
                     /locus_tag="LQB69_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3525112.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            31428..31847
                     /locus_tag="LQB69_000931"
     CDS             31428..31847
                     /locus_tag="LQB69_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL3525113.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            31859..32329
                     /locus_tag="LQB69_000932"
     CDS             31859..32329
                     /locus_tag="LQB69_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3525114.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            32346..33125
                     /locus_tag="LQB69_000933"
     CDS             32346..33125
                     /locus_tag="LQB69_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3525115.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            33112..33933
                     /locus_tag="LQB69_000934"
     CDS             33112..33933
                     /locus_tag="LQB69_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3525116.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            33950..34999
                     /locus_tag="LQB69_000935"
     CDS             33950..34999
                     /locus_tag="LQB69_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3525117.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYIQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            35012..36016
                     /locus_tag="LQB69_000936"
     CDS             35012..36016
                     /locus_tag="LQB69_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3525118.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            36649..37668
                     /locus_tag="LQB69_000937"
     CDS             36649..37668
                     /locus_tag="LQB69_000937"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL3525119.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        12307 a         6644 c         7682 g        11058 t
ORIGIN      
        1 aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt tttagttttt
       61 gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa atgaatcatg
      121 tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa acagtccatt
      181 tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga gaaaaaattc
      241 cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt cacagaaatg
      301 acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat caatcctcta
      361 aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt tgaactccct
      421 gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc agtttgtgtg
      481 acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct gctcaaaaag
      541 aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca acattatttt
      601 gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat cggtgagcca
      661 tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc caaaggattg
      721 gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa aatcaaggaa
      781 ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc gaataatgag
      841 gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt gatggaagct
      901 gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat catgttgaat
      961 caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa agataagaaa
     1021 aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga tcaatattct
     1081 cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa cggcgtaaac
     1141 tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca attgcgaagc
     1201 aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt caattaccat
     1261 aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa aagattctat
     1321 agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa gaaatgtcct
     1381 caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga ttttcttata
     1441 tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg cctgtttaaa
     1501 tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca tggctttatc
     1561 taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg atactggaag
     1621 ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct gatccatcat
     1681 caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct ccattttttc
     1741 tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt tattgttaac
     1801 taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt caattgcata
     1861 tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca agagacgatc
     1921 aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat catcgccacg
     1981 agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg cccataagta
     2041 tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag gtggttttga
     2101 aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata ccgcagtgac
     2161 gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta gtttttcacc
     2221 aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga cgtctgcttt
     2281 ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat tacccccgta
     2341 ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt tgttttctgc
     2401 ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt ctttgacgat
     2461 tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga aaacactttg
     2521 actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa gagtgatcga
     2581 tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt gatgaataag
     2641 gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag tacaaaaaca
     2701 ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt ccctttgctt
     2761 ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca ggttttattt
     2821 gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga tgaacaaaca
     2881 gagttttttc ctccaattga tggctttcct gatctttcct attttgaata aagtgtttct
     2941 gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat actgattggt
     3001 agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta gaataagcaa
     3061 gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc agtattgaaa
     3121 gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga cagctcggca
     3181 gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt agaccaagat
     3241 gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg ggaattgcat
     3301 ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt agtcaattac
     3361 ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac ggatgattta
     3421 gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga taaagtggct
     3481 tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg ttataaaatc
     3541 gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat ggcaaaaacg
     3601 attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt aatcagcttc
     3661 aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat tccaacttat
     3721 ttccgaagaa gctacttttg gaacaccgtg taccagaggt tgtgaaaaag tcgcttagtt
     3781 tcgagtagta agaaaaattt tgtgaaaatg gctttctaca tttttgcata aatttggctt
     3841 aataccgaga aactggcttt tgaacaccgt ttattcagat aagggactgt gacaagactt
     3901 ttgtcacagt ttctttttgt ttattctttt ttatctttgt atggcaataa tagacaatat
     3961 aacgaaaatt atttaatggc ttttggaaga ccatggaaaa ccttgtacaa agctattttt
     4021 tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt ttatgaaaaa gacaaaaaaa
     4081 agtgttgatt ttttaataat tattcattaa actcttaaac aacttcagaa aatattaatt
     4141 attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa gaaatttaca tttggtttgg
     4201 ttgcggtatg cggccttgta cttgctggat gttatggcgg cagttcagcc acaagcagta
     4261 gttcagcaaa aagcggttca gcagatggag gaggcgtatt taacttagtc gtgcctcaag
     4321 aaatgcctac agctgactta tcagttgcaa cagatacaat cagttttaca gcattgaata
     4381 acgtttatga aggaatttac cgtcttgatg aagacagcaa accacagccg gcaggagcta
     4441 gcgagttggc agaagtcagt gaagacggat taacttacaa aatcaaatta cgagaagacg
     4501 caaaatggtc aaatggtgat cctgttacag cagcggatta cgtttatggc tggcaacgaa
     4561 cagtagaccc agctacagct tctgaatatg cttacctgta tgcgccagtt gaaaatgcag
     4621 aagacattac agctggaaag aaagataaat cagaactagg gatcaaagca gttggtgact
     4681 atgaattaga aatcaaattg acgaaacaaa caccatattt ccaatatctg cttgctttcc
     4741 catcattttt cccacaaaaa caatccgtag tggaagaaaa tggggatgcc tatgcatctg
     4801 caagtgataa agctgtttat aatggaccat tcacattaga agattttgat ggaccaggaa
     4861 cagatacaga atggacttat aagaaaaatg atcattactg ggacaaagat acagtaaaac
     4921 tttctgaaat caaagtcagt gtagtaaaag aatcttctac tgcattgaac ttattcaaag
     4981 atggccaagc agatgacgtg atcttgtctg gtgagttagc acagcaaaac gcaaacgatc
     5041 cagcttatac ttctgttaaa gaagcgcgga caagctatat cgaattcaat caacgtgaaa
     5101 agaattcacc attcaacaat gtgaacctaa gaaaagctat ttcttattct atcgatcgtg
     5161 aagctttagt aaaacaagta ctaggtgacg gatcagttgt ctctacagga ttgatcccat
     5221 cagatatggt aaaaagtcca acaacgaacg aagactttgc aaaagaagct ggaaaattag
     5281 tttcttatga caaagataaa gcaaaagaat attgggaaaa agcgaaaaaa gaattgggcg
     5341 ttgattcttt agaatttgat ttgatggctt ctgatgatga ttcatcgaaa aaagtaatcg
     5401 aatatgttca aaactcaatc caagaaaact tggatggcgt aacagtcaaa ccaacacctg
     5461 ttccattctc tgtacgtctt gaccgctcaa cttcgggtga ttttgacgca gtactaggcg
     5521 gatgggctgc tgactatgca gacccaagca gctttactga tttgtttgtc acaggtaatt
     5581 catacaaccg tggtcgctgg agcaatgcag aatatgacga agcagttaaa gcagcttcat
     5641 caacagacgc tggcgatgaa caagcccgtt gggatgattt ccaaaaagca atcaagatca
     5701 tctctgatga tatgggtgtc gttccggtgt accaaaaagc agaaggtcac ctagtgaatg
     5761 aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc atgggattac aaatggacat
     5821 acgttgaaga ataatcgtta ataccgtgag catgagaaaa aacgaggctg cggcaaatgt
     5881 cgcaacctcg ttctcatatt cacttatcct aacaaagaaa aaagcatgaa aatagcagaa
     5941 agagcaatta gtattccggc aatgatcagc caaaagctgt acataccgta gctggcagac
     6001 gagagcgagg aaaattctgg ttttttcttt cggttccttg ttcgtgcaag atggaccgat
     6061 cgctgaaaat gatcgaagaa acccgaagaa aagacccata aaaaaccgcc gataatcaga
     6121 aacggcaatg accataagaa tagatcatta gataattgaa tgagtgtaaa ggaatggttg
     6181 atgagatttt ttattaaaat aaccagaatc agtagaaaag ctactgcgta ggggatagat
     6241 ttttttttca tttgattgct cctttatttt tggttcatta tctaataatg tagcgaaaca
     6301 tctgtcagaa gtcaaaagct agggggaaac attagtgaat agttatatca aatatgtatt
     6361 aaaacgtgtc tttttcatga tcatcacgct ttggttaatt gcaacaatca cctttttctt
     6421 gatgcaattg ctgccgggca cgccatatac gaatcaggaa cgtttgagtc ctgaaacaat
     6481 cgagatgcta aataaacaag tcggtttgga taaaccagtt atcgtacaat atgggattta
     6541 tctttctaat ttgcttcaag gagattttgg aatttccttc caatttaaaa accagccagt
     6601 cgcacatttg ttagctggaa gaatcggacc atccttgcag ttagggttac aggcaattat
     6661 ctttggaaca gtcctaggga cgattttagg aacgatctct gccatgaagc aaaatacatg
     6721 ggcagataca tcttctacac tcgtagcgat attaggtcgt tccattccaa actttgtatt
     6781 tgctgtttta ttacaatata tctttgcaat caaattacaa gttttgccaa tcgcaaaatg
     6841 ggatggcttt gtgtacacaa tcttaccaac aattgcgttg gccatgtcgc cgcttgctga
     6901 ctctgctcgg tttatccgta cggaaatggt ggaagtactg catagtgatt atgtggaatt
     6961 agcaaaagca aaaggattga gccgttggga aatcgccttt aaacatggtc ttcgaaatag
     7021 tttgattcca ttgatgactt tattaggacc gttagctgta gcattgatga ccggatcact
     7081 ggtggtagaa aatatctttg ctattcctgg tatcggggaa caattcgtta agtcaatcac
     7141 aacaaatgat tatcctacga tcatggctgt aacgatcctt tactcattta tgctgatttt
     7201 tgttattttg atcgtggatc tattgtacgg tctagttgat ccaagaattc gtgtttcaga
     7261 agggagccga ggataaatgg aaatgatacc aaagcgttat gaaacagttg cagatatccc
     7321 tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa gaacgtgaac agattactgc
     7381 accttctttg aattttatcc aagattcatg gcgccgttta aagaaaaaca aagctgccgt
     7441 tatttcaatg gttttactat tggtcatcat tttgatctct gtgatcacaa tctttgtttc
     7501 tcctcatgat ccaactgccc aaaacgtggc ttatatcaat ttgcctcctc gtattccggg
     7561 aatcaatatc gatggattga atggaaaagc aatggttggt ggtgaacttg ttgataaata
     7621 tgctcaagca aacgtccctt caaacgtaaa tttcttttta ggaacagacg gtttgggacg
     7681 tgatgtatta agccgcttat ttatgggaat gagaatctcg ttgctgatcg catttattgc
     7741 tgcattacta gatgtaacga ttggtgtagc ttacgggttg gtttctggtt tgttaggcgg
     7801 acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta tcaggaattc caaacctcgt
     7861 tgtcatgatt ttgatgttgg tcgtttttga accaggaatt ttttcaatcg tggctgctat
     7921 ggccatcaca aactggatac caatggcacg tatcgttcgt gcacaaacaa tgaaattaaa
     7981 agatcaagaa tatgttttag ctggattgac attaggtgaa tcaagaatga aaattgcatt
     8041 caaacatatt ttgccgaata tctctagtgt cattattatt caaatgatgt ttagtattcc
     8101 atctgctatt ttcttcgaag cctttttaag ctttatcggt ttaggtctta cgcctccgtc
     8161 agcttcatta ggaacgatgt tgagcgaagg gtacaagaca ttcctgtatc tgccttatct
     8221 attatggatt ccggcagtaa ctttatcggt tatcatgatt ggctttaact tacttgctga
     8281 tggattgcgt gatgcctttg atcctaaaat gaaagagtga agacgatgac gaaaatttta
     8341 gaagtgaaag acttagaaat ctcttttgat acgtatgctg gaaaggtacg agcgattcgc
     8401 ggtgtcaatt ttcatttgaa taaaggagaa acattggcga tcgttgggga atctggaagt
     8461 ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt caagcaacgc caatatagat
     8521 gctggtcaaa tcctctttaa aggtaacgat atcgttggaa aatcagaaag agagatgcaa
     8581 aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc caatgacctc tttagatccg
     8641 acgatgccca ttgggaagca agtcgctgag tcccttagaa agcataataa agtttcaaaa
     8701 aaagagggac aaaaagctgc attagaattg cttaaattag ttggaatacc cgaagctgag
     8761 aaacgtatca acagttatcc gcatcagttc tcaggaggtc agcgtcaacg gatcgtgatc
     8821 gcgattgcac tggtatgtta tccggaaatc ctgattgctg atgaacctac aacagcgctt
     8881 gatgtgacta ttcaagcaca gattttggag ctgctgaaag aaattcagac aaaaatcgat
     8941 acatcaatta tctttatcac tcatgacctt ggtgtagtag cgaatgtcgc tgatcgtgtc
     9001 gctgtcatgt atggaggaag aatcgttgaa gttggaacat cggaagaaat tttctacaac
     9061 ccgcaacatc cctatacttg gggactgctt ggttcaatgc cgacattaga tagtgcgaac
     9121 gatcgattgt atgctattcc tggttctcca ccggatttat tgaacccgcc aaaaggagac
     9181 gcattctatc caagaaatga attcgcgatg aaaatcgatg cagaacaaga accaccattt
     9241 tttgaactat caaaaacaca caaggcagca acttggctac ttgctccgca ggcacccaaa
     9301 gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct ttaaagagaa acaaaaacat
     9361 acttacggtc aaggaggaat tgctgatgcc aaataaacta cttgaagtta aaggattgaa
     9421 acagtatttc aatgtaggaa aaaagaatga agtacatgct gtaaatgaca tcagctttca
     9481 catttatgaa ggagaaacat tcggtttagt tggagaatct ggaagcggaa aatccactac
     9541 tggccgtacg atcattcgct tgaatgaacc tactgatggt gagatattat tcgacggtca
     9601 agatataacg aagatcaaag ataaaaaagg tttgacgaaa ttccggcatg atgttcagat
     9661 gatctttcaa gatccatacg cttcgttgaa tcctcgtatg aaagtcagag atatcattgc
     9721 cgaaggaatt gatgttaatg gactagctaa atcatctgaa gagcgtgcaa aaaaagtcga
     9781 tgatctattg agaactgttg gattgaatcc aagccacggt actcgttatc ctcatgaatt
     9841 ttcaggtggg caacgccagc gtattggtat tgcacgagca ttggcagtaa aaccgcgttt
     9901 tatcatttgt gatgaaccaa tttctgcact agatgtgtcg atccaagccc aagtggtcaa
     9961 tttgttgcaa gatctccaaa aagaacatca attgacctat ctatttattg cacatgattt
    10021 gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg cataacggct tgttattaga
    10081 gatgggaaca agtgatgaaa tctataatca cggtgttcat ccttatacag aaagtttgtt
    10141 atctgctatt cctctaccag atccagacca tgagagaaaa cgcagaagaa tcaaatatca
    10201 acctgaacca gatgacggac aagtacgtca acttcgtgaa atcgcaccag aacattttgt
    10261 ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa ctaaaacgac aaaaggaatc
    10321 tcttctggtt gctaactagg agggacatag atgctaaaag cttttaaatt ccgtatgtat
    10381 ccgacagaag aacaaaaaca gcaattgatt cgtacttttg gatgtgctcg atttacctat
    10441 aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa caggagtagc caatttttcg
    10501 ctcacacccg ctacattaaa aaaagaatac ccattcttaa aagaagtaga cagtcttgct
    10561 ttagcaaatg cacagcttaa tttggatcgg gcttttcgca attatttcaa gggacgtgcc
    10621 agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat cttatacgac caacaaccaa
    10681 acccgtacga tctatctgga aaatggctac ttaaagttac caaaacaaaa agaactaatc
    10741 aagataaatc agcatcggcc agttgaagga tcgattcgct ccgctacgat ttctgctcga
    10801 tataatgaag aattttatgt cgcactactt tgtgatgttt cgccagtaaa aaaagaatct
    10861 ttagctaaat ggatcgggat cgcctatcat ccgaagacat tgatccaaac atctcgacct
    10921 ctagaagtaa ctttaccaaa atttcaccag acagaagaaa agctgcaaca tgcacagcgg
    10981 aaattgaatg tgaaagtccg ctccgctcac catagaaaga tcaggttaga tcaggcaagc
    11041 aattatcaga aacaaaagag aaaagtaatg gatctttatc tgaaacaaaa gaaccagcga
    11101 gaagattatc ttgaacaatt atctgggaaa ttggtcaaac aatatgacta cttatttgta
    11161 gaatcttttc caaaagaaga ggcacatgct gatttttcta ttcacgactg gcataaatta
    11221 ataacaaaat tgcgttataa gtcccaatgg tataataaaa aatttctttt gatcaatacc
    11281 gacggagcag aagaaagcaa ttccgtaaga aagagccaag tagtagaaaa aatgggcaga
    11341 cattcggtta tcaagggata aaaaatgaat gaaactgata ggagcgttag ggttagcctt
    11401 gttaataaaa agtcttaatg acttttgttt ctaggaattt ttcttttgaa gaaaagctca
    11461 ctaattatga caaaccaata aaacaatagc agaaaaagtt cgtacgagct ttttctgcta
    11521 ttgttaattt tttattcaga aagtttacga atacatcaaa attctgaaac aaaaaacgta
    11581 actcacaaag agttgttttt gcgttttttc ttgaatgatt aggtctgaat cttttgctca
    11641 caaccttatt catggtgccc cggcttgagc tgatagattt ttgaaggacg gccgattcct
    11701 acaggacgtt caccgatctc atcgaaatat tggagcatag ctttcttaaa attggaatga
    11761 tcgatcgttt tataatcgat ccctaagaat ttagcaaata cttttcgagc ttccgtaatc
    11821 gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt gttccatttt attgactaca
    11881 cggttgaatg ctttcaagat gatttgacta tggtcaaaag ccaaagtatc tttgccagaa
    11941 gactctccag tgactaaatc aagcgagata gccacttctc cgctagataa atggatcttg
    12001 ttactatgac gttctagtgt gaaccagcgt acttccttgg catcatctcc ggcgattagc
    12061 ggttcttctc caataaaggc taagtagctg actgtgacga cccagcctcg cggatctcga
    12121 ttaggcgtac tgaatgtatg cagttgctca atattttctt ttgaaataac cacaccagtt
    12181 tcttcctttg tttctcttaa gacgctttct ccagttgact catccttttg gacaaagcca
    12241 ccaggcaatg cccatgaatt tcgataagga tgcccttttc tttggatcaa caaaactttc
    12301 agctgatctt cttctttgtt atagcacatc agtacgatat cgacagtcaa agagggcttg
    12361 tcatattctg gacgttcctg tttttgatac cagttaagaa attcttcttc acttgcttta
    12421 tgttcataat ataatttttc ttcttcttta gaagcaaaac ggttcatctg accacctcca
    12481 ttgacagtaa aagtactttt taccttattg tagcagactc tgaaaaaaaa ccaaggattt
    12541 acttgaaaaa tctaggttat catttaaata tgaaaaataa atggaagtat gagattgaat
    12601 aaaaagaaaa gcagataaaa cggcaatact ttataccgct cttcacgaat ggcaaagaag
    12661 ccacaaataa cgatatatac gtcaatgata aaacgcaaaa tccaagctgc tgtcataaaa
    12721 ctgttcaaat gcccatggag gttaaaataa ccatacatac gaatataaaa atagctgaat
    12781 attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa aaaaaagatg tttcttctgc
    12841 atgattgttc cagtccctta ctaagataga tagtctttat tataccacgt gtgaggggag
    12901 aaataaaaag ccgtttttta gtagatatga aacaaaacaa aaacccgcat ttattcaacc
    12961 tattcatcct tttaaaggat agattgaacg tagatagaag tcgtttatcc agtctttttc
    13021 attttgaaaa aaagctaaca gcatgctttt tttatggtat actagcaagg aaacgaattc
    13081 gcatcatgcg ataaaggagg aagagccccg tggcagaaaa ggaaactttt tatatcacta
    13141 cgccaatcta ttatccaagc ggcaaacttc atatcggcaa ctcgtatact acgattgcat
    13201 gtgacgcaat tgctcgttat aaacggttga tgggctttga tgtgttttat ctcactggag
    13261 tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga actaggtgta aaaccgcaag
    13321 aatatgtaga taaaatggcg gctgatgtca agaaattatg gaaaacgctt gatatcagtt
    13381 atgataaatt cattcgcacg acagatgatt accataaagc agctgttcaa aaaatatttg
    13441 atcgtttatt agaacaggga gatatttact taggtgaata tgaaggctgg tactctgttt
    13501 ctgacgaaga attctttaca gaaacacaac ttgcagaagt gtacaaagat gacgaaggta
    13561 acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga attggtaaaa gaagaatcat
    13621 acttcttccg catgagcaaa tacgcagacc gtctattgga atactatgaa gaacatcccg
    13681 aattcatcca gccagaatct cgtaaaaacg aaatgatcaa caactttatc aaaccaggat
    13741 tagaggatct tgcagtttca cgaacaactt ttacttgggg agtaccagtc aagagtgatc
    13801 cgaaacatgt ggtgtatgta tggatcgatg cattatccaa ttatattact gcactaggat
    13861 atggctccga agatgaaagt ctattcgaaa aatactggcc tgcagatgtt cagatggtcg
    13921 gtaaagaaat cgttcgcttt catacgatct attggccaat catgctgatg gcccttgatt
    13981 tgcctctacc taaaaaagta tttgggcatg gctggctatt gatgaaagac ggaaaaatgt
    14041 ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt ggaacgttat ggtttagatg
    14101 cactccgtta ttatttgctt cgtgctgttc cgtttgggtc agatggtgtc tttacgccag
    14161 aagattttgt ttcccgtgtc aattatgatc tggcaaatga tctagggaac ttattgaatc
    14221 gaacgattgc tatgatcaac aaatattgtg atggactcgt tcctgactat gcctcacttg
    14281 tcactccatt tgatagtgaa ctgtctacga cggctgctaa cgttgtcgga cgttaccatg
    14341 atgcaatgga aaaaatggaa ttcaatacag cattggctga aatctgggtg ctgatttctc
    14401 gtgcaaacaa atacattgat gaaacagaac catgggtatt agcaaaagac gaagaaaaga
    14461 aagctgaatt gaatagtgtc atgatccatt tagctgaatc tttgcgtatc gcagcgatat
    14521 tacttcagcc aatcatgaca gaaacgccag agaagatctt caatcaacta ggattggact
    14581 ctgaaacaat gaatcttgaa ggattacact ttggcgaatt tccttcagga acaaaagtag
    14641 ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga agaagaagtt gcttttattc
    14701 aagaaaaaat gtctgaaggt actcaaacaa acgaagatac agtcaaatgg gatccagaag
    14761 aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt tgatgtattt gaaaaagtag
    14821 aactaaaagt agcagaagtc atcaactgtc aaaaagtaga aggggcagat aagttgctgc
    14881 aattccgact agatgctgga gacagccaag accgtcaaat cttgtcagga atcgctgaat
    14941 tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt cattgtagca aatctgaaac
    15001 cacgaaagat gcgtggacaa atcagtcaag gaatgatcct ttctgcagaa gcaccagatg
    15061 gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa tggctcagaa atcgcataga
    15121 aattcaaagg gggtatgaca ctagttgtgt cataccccct ttttccgaaa tttgagaagc
    15181 aattttcgta aattccttct tattacttga agccaatcat ataatacaga aacagagaac
    15241 tatccaaaat aaatcctaga aaaagaagta cgagcttcgc tgatttttgt taatttagaa
    15301 taatactaat aggaatcgta aaaacagaaa aaaagcataa tgataaaggg tcgggcagat
    15361 tttaattgat tcatctggta ctctttttta ttgtccttac attcataaag tttgaatgca
    15421 atgactttat tacatctata tttatatcta acagtagcgt tgatgtgtta attataagtt
    15481 ctatcacttt atgttcaatt agtttgtgct cttatatgtg tgattgaaaa tatagataaa
    15541 aaaacaatca tcggctttga cttcgatgat tgttttcttt attttgtaaa aatcctatca
    15601 cttgatttta tggtaacctt ttatttttgt cttcaaacgg cttggtaaga gagcagtaag
    15661 cagtgcaaat gggatcgaaa agacatacca gaaaatcgta cgtccgatac cataatgttg
    15721 atagagcttt gtcaacatca cgtgtaacaa gatcaaccct aaaagcaagg agccagctat
    15781 aaaccaatga ttatcccaat gcatgacttc actaagtgcc atcactccaa aaggatagaa
    15841 ataaaaatag atcccgacta aggtcatccg ccagttcttt gtaaatgttc cgatattcaa
    15901 taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga aaaagcccaa ccaaaaagga
    15961 atacaacatc ccccacaaga tcatcacagg acctttgatc aatgcaacga ttgcgatgaa
    16021 caaagtcagc aatattagtt tttgtgataa cgttgcaaca gtcattaatc caaataaaat
    16081 taatgtgaac caatgccaat gggactgctg ttgttcttta cgggaaaaat cgacccggtt
    16141 ggataaataa tctgctaatt tcggataaga acgcttaaaa tccataaact gttcctcctt
    16201 cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga aatacatccg tcttaagagg
    16261 gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt aaaatattct cagttatact
    16321 gaaaattata ccaaatttca aagaagaaag aggatgggaa tgaaaggcaa actaaaacga
    16381 gaaatcaatc tgtttggcgc attagctacc gttatgggaa ccgtgatcgg tgcaggcgtc
    16441 ttcttcaaaa ctgctgctgt tactgctagt actcagtcta caagtttgac attgctagct
    16501 tggcttttgg gtggtttctt aacgatctgt gctggattga cagtggctga attagctaca
    16561 gcaatcccgg aaactggtgg agctgtaaaa tatattgaag ctgcgtatgg aaaattgccc
    16621 agctttttat taggatgggc gcaaagcttg atttactttc ctgctaatat tgctgcatta
    16681 tcgattatct ttgcgacaca attgacgaac ttattacaat tatctaccga ttatcttttg
    16741 ttgattgcag tgatcaccgc tgtttctgtg acagggttga atttgttagg gacaaaagtc
    16801 ggtgcttctg tacaatcagt gacgttgatt gttaaattaa taccaattgc tgtgattgtc
    16861 atatggggat tattgacccc aggacaagga actgtccagt tatttccaat cgaagctgga
    16921 aaagatgtta cttttgtcga aggattgagc agtgcgttgt tagcaacgtt gtttgcttat
    16981 gacggctggt taggtgtcgg tgcaatggca ggggaaatga aacggccgga aaaagatttg
    17041 cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg tttatctatt gatcaatttt
    17101 gtttttttga aaactttgcc gattgatcat cttgccggta atttgaatgc agcttctgaa
    17161 gcatcagacg tcatttttgg aggaatcggt ggaaagctgg tcactattgg gatcttgatc
    17221 tcggtatacg gtgcattgaa cggttataca ctaactggga tccgcgtgcc ttatgcgatg
    17281 gctttagaag acgagctacc tttcagtaaa cagctgacaa agctttcaaa gaagtttacg
    17341 gttccttatg ttcctgcggt tttccagtta gcagtagctt gtattatgat gagccttggt
    17401 tctttcgact ttttaacaga catgttgatt tttgtgatgt ggctattcag tttactgatt
    17461 tttatcggtg tctttatttt aagaaaaaaa gcgccggaat tgccacgtcc atataaagta
    17521 ccactttatc caatcgttcc gattattgcg attctcggtg ctattttcat tttaggtatg
    17581 acgatgatga cgcaaacgaa attagcattg atcggtatag gtgttacctt gataggtatc
    17641 ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt aaaatatttc aacaacagac
    17701 agaaaaagta cgaactaggt cctctagatc ttcgtgcttt ttctatttca ataaaacaag
    17761 ttcttctatt ttgatttttg ctacttctgc aagtagtgct tatgtgctaa agtaagaatg
    17821 aaaaggagag ataaacatga tatttgattc tcatacccat ttaaatgctg aacagttcaa
    17881 tgaagatatt ccagaaacga ttcagcgagc acaagaaatg ggggtaacga aaatggcggt
    17941 cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa ttaagccatg actatccaaa
    18001 tatatacagt attatcggct ggcatccgac agaggctggc agttatacga aagaaatcga
    18061 aaataaatta caagaacaat tgactttacc aaaggttgta gcattagggg aaattggctt
    18121 ggattatcat tggatggaag acccgaaaga agtccaagat aaagtatttc gcagacagat
    18181 tgctatcgca aaagaaatga acctaccaat cagtattcat actagagaag cacttgaaga
    18241 cacgtatcaa atcctaaaag aagaagatgt tcgagatatc ggtggaatca tgcatagttt
    18301 cagtggtgat tatgaatggg caaaacgatt tcttgactta gggatgcata tttcatttag
    18361 cggtgtcgtc actttcaaaa aagcattaga cgtccaagag gctgcagcaa atgtaccaat
    18421 cgaacgatta cttgtggaaa cagatgcccc ttatctggca ccagttcctt ttagaggaaa
    18481 gcgaaatgaa ccaggctata ctcgttatac agtagaaaag attgctgaac ttcggaatct
    18541 tccgacagaa gaagtggctg cccaaacatg gacaaatgcg caccgattat ttggtctaag
    18601 cgaacatgga taagatgaag atccaagaga ttatagtcgt tgaaggaaaa gacgatacaa
    18661 gaaggctgcg ggaagtagtt gatgcagata cgatagaaac aatagggtct gcaatcaatg
    18721 aagagatcct gatgcaaatt gaacatgccc aagagactcg tggagtgatc gtttttaccg
    18781 atcctgattt ttcaggagaa aaaataagaa aaatcattat ggaagctgtt cctcaggcaa
    18841 aacatgcttt tttgccgcgc acacaagcta aaccaaagaa aaagggaagc cttggtgtcg
    18901 agcatgcaag tgatgctgct attttagaag cattgaaaaa ggtcgtgacg ccagacattt
    18961 cccaaacggc tgtttctgaa atcactagac aagacctttt agactatggt ttaatagctg
    19021 gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga gttgcgcatc ggttacacga
    19081 acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat tacgccgaaa gaactcgccc
    19141 aagcaatgaa aatagtggag gaacaacatg agtgaatatc gtgaaattgc gacaccctct
    19201 agaacaaaag agattttatt gaaacatggt ttttcattta aaaaaagttt aggacaaaat
    19261 tttctgactg aaccaaatat tttaaggaaa atcgtagaaa ctgcgcaaat agatgaacat
    19321 acgaacgtga tcgaagttgg accaggaatc ggtgcattga cggaacaatt agccaagcat
    19381 gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga tccctgtatt ggctgatacg
    19441 atgcaaccct atgacaatgt gactattgta catcaagata ttttaaagac cgatttgtca
    19501 acagctgtta gagaaacatt tcatgaggag cttccgttaa aagtcgtagc gaatttacct
    19561 tactatatta cgacaccgat catgatgcat tttcttgaat cagatttggt tgtggatgaa
    19621 ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct ctgctgaacc aggaacgaaa
    19681 gcttatggtt cattatcgat tgctgttcaa tattatatgg aagccagttt agcattcatc
    19741 gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt cagcgatttt gaaactgaca
    19801 agaagagata taccggcagt cgaagtgaca gacgaaaaag catttttccg attgacaaaa
    19861 gcggcatttc agcaaagaag aaaaacgctt tggaataact tgcagcattc gtatggcaaa
    19921 gatgaccaga caaaagcatg gttagcaaaa agcttggaaa cagctggaat cgatccaaaa
    19981 cgtcgtgggg agactttgtc attacaagaa ttcgccgcat taagcaatgc gatgtcagaa
    20041 aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa aaagctgtcc tgcatcaagt
    20101 aataagaaca gccaaacaaa aatagttcct catgtttttc gtttgtttgg gcttattacc
    20161 gcagatgcaa gcttttgaac accgtttaag aaagaggttg tgggcctgcc gtttaactct
    20221 aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt tttgagttag ttggtcttat
    20281 tacttgatgc aagcttttga ccactattat gccgaacaag gagatgtgac acaaattttg
    20341 tcacatctcc tatttttgtt tcttgatcaa aaaattctta tacttgatac tcacactcat
    20401 tttgtatgga tttagaaaag aatatgtgtc ttgacagtta ttagtaagtg agttatacta
    20461 agtcgacatt tatgtgtcaa gacatattag gagagagaaa aaatgagaaa aaattcagtt
    20521 cgaaaactaa cgatttctgc tttattgatt gggatggggg tcattatccc aatggtcatg
    20581 cctaaaatca tgattccacc agcttccttt actttggcgt ctcatgtgcc attatttatt
    20641 gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc taggtacgac atttggtttc
    20701 tttttaacaa cgccggttat catcgcattg cgagcattgt cacatcttgt gtttgcgttg
    20761 attggggctt ggtacttgca aaagcatccg agtatcgttt taaaaaatgg acagtttact
    20821 tttgctaatt ggcgtttcca agggttcaat tttgtgatcg gtgtgattca ttctttagca
    20881 gaaatgctag tagtcagcat cttttacttt attggtaata tgcctgaaac atattattct
    20941 caaggatatt tttatacagt attcatcttg atggggattg gtgggcttat ccacagttta
    21001 gttgattata atattgctta ttttatagca ggaacactca gcaaatattt tgatatcccc
    21061 gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga ttcaagggta cgcgaagaaa
    21121 acgacatatt agacatgatc tctcaaaaaa aaaactctca tctagtaatg gataataaat
    21181 cctctaagat gagagttttt tgtcctaaaa agagctgcac caacatcttc tggcacagct
    21241 ctaacagggg gcaatttatg gaagtttgat gacttgttct gtttctttat caaagaagtg
    21301 gcctttgctg atgttgaagg caaggtcgac tatttcgcct ggtttatgga aatctcttgc
    21361 atctactttt gagatgaact cagtagaacc agtttttgtg taaagcatcg tttcagctcc
    21421 aagtaattcg gaaacgacaa cttctgattt cactacggct tcaggtgaag cttcaagggc
    21481 tacttgttca ctgtggatgt cttctggacg aataccgaag ataacttctt ttccttcata
    21541 acctttttca actaatactt tgttttttcc ttcaggaata cgaagcttaa gcccatgtcc
    21601 atctgtgata acaccatttt tcaatgtcac attaaagaag ttcatggcag gagatccaat
    21661 aaagccggca acgaacatat tgacgggtgt atcataaact tctttcggtg taccgatttg
    21721 ttggataaat ccgtctttca tgataacgat tcggtcagcc atagtcattg cttctgtctg
    21781 atcgtgagta acatagatgg tcgttgtttc aagacgctgg tgaagtttgg caatctcagc
    21841 acgcatggct acacgtaatt tagcatctaa atttgataat ggctcatcca ttaagaagac
    21901 ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt tggcgttgtc caccagataa
    21961 agcagctggt tttcgtttca agtattctgt cagacctaag atctctgctg cattgtccac
    22021 acgttttttg atgtctgctt tatcatattt acgtaatttc aagccaaaag ccatattatc
    22081 aaaaacagtc atatgaggat acaatgcgta gttttggaaa accatggcaa tatcacggtc
    22141 tttaggtgcc acgtcattca tgacttttcc gccgatattt agttctcctt cagagatatc
    22201 ttccagtccc gcgatcattc gcaaagtagt tgactttcca caaccggaag gaccaacaaa
    22261 aacgataaat tcacggtctt tgattgatag attaaaatca gttacagaat aattttctgc
    22321 attgtcatat tttttatata cgtttttcag agccatttct accataatac gttccacact
    22381 ttctttttta gttttattaa ctatttaaag tataaatgaa agcgttaact aacttcaatg
    22441 tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg ctagttttaa gctttaataa
    22501 atatccataa gaaataaaaa ctgatacact ggagtaaata agtatgttaa caatatcaaa
    22561 aggagtgtct cttatgaaaa tcgctttgat cgctcatgac cgaaaaaaac ctatgatggt
    22621 gaaattagca acagcgtata aaacaatatt ggaaaaacat gaactttttg ctaccgggac
    22681 gactgggcag aggatcacgg aagcaaccgg cttgccgatc catcggttta aatccggacc
    22741 gttaggcggg gatcagcaaa ttggagccat gatttcggag gatcagttag atttggtgat
    22801 ttttcttcga gacccgctgg ctgcacagcc ccatgaacca gatgtaacag cacttatccg
    22861 tttatctgat gtctatgaga ttccgttagc tacgaatatc ggaacagctg aggtgctact
    22921 aagaggactg gaagccggct tcctagattt tcgaagaatc gtccatgaaa tagacaagca
    22981 tccactttca ttttgatgga taagcgtatg gaaaaataac gtttaatgtt cgcctatatg
    23041 tagctataaa agctaaacaa actgataata tgcgatataa tacgaacgta ttggataata
    23101 taaacaataa tattagtttt tcgtatttgc agtaaatgtg aaaatatgct atagtttctg
    23161 tgggaaaaga tcccaaaaat aatacagagt agaaaataaa gcgttaagtg ctaagaggat
    23221 gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt tttattttcg cattcgctgc
    23281 atgatacgat gtagcagctc ttaaaggaga tgctaaaaat gaagaaacac cggttagacg
    23341 cacgtatgat tgctattatg ggcttattga ttgcattgat ggtcactttg tcccgactgg
    23401 tagcaatcga gaccccattt attaaaatca gtgtcacatt tattccacaa gtgatcatgg
    23461 gcattctttt tggtcctttt tggtcaggga taggcgcagt tttagctgat ttagtaggaa
    23521 tggccctctt ctcgaaatct gcattcttta ttggatttac attgaatgca tttattgaag
    23581 gagcaatcta cggattcttt ttctatcgaa aagagatcac ttggaaaaat gccattctgg
    23641 ctacgcttag tgtcacgctg atcatcaact tatttttgac accattatgg cttgcgttga
    23701 tgtaccatgt gccattgttc agctgggtcg tttgggcgcc ccgcctgtta aaaacagtaa
    23761 tatggttacc gattcaatca attgccattt attacgtagg acgtagtatt ccttacaaaa
    23821 aaattttaag aagtttagcg atccacgcta aataaaaaac aagcagtctt cccatcccag
    23881 aagactgctt gtttttattg tgctgtgaat tgtgcttgat agagccgtcg gtaataacca
    23941 tctttttgaa gcagctcatc atgggtgccc atttcgacaa tagctccctg atccatcact
    24001 aatatctggt ccgcattttt gattgtggac aggcgatggg caatcacaaa actggttttt
    24061 ccttccatca ttgctaagaa ggcttgttgg atttttttct ctgtcaatgt atcgactgag
    24121 ctggtagctt catctaagat caacataggg ggattactga tcatcgttcg agcaatcgtc
    24181 aacagttggc gttgtccatc tgatatcttt aacccttgac cgccaatctt agtatctaat
    24241 ttttccggca aacgttctac aaattcatac atataagagg cttttagggc atcattgatt
    24301 tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt ctcgcaatgt actgtcaaat
    24361 agccaagtat cttgtaacac cattccaaaa cttttacgta atgtatcacg ttggatagag
    24421 cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg tgtcataaaa acgcatcagc
    24481 aaattaacaa gtgtggattt tccggcccct gtttttccta ctatagcaat ggtttgacct
    24541 ggataagcag taaaattgaa atcttcgatc aaacgctgct tggggtcata agaaaaagaa
    24601 acatgatcaa aagagacttt tcctttgata tttttgggtt ccaaggcata ggcatctggc
    24661 aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa
    24721 gcggtttgca gttgtgtcgt gattccagaa agttcgataa atggtttaga aaactgactt
    24781 gagtagatcg taaagctgga aatcacacca acagtgattg acgaaccact cttcaatgcc
    24841 aataggccgc caactaaacc aatcgataaa taagcaaggt gatctacaaa acgagaagac
    24901 gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt tttcatatag atcagcattt
    24961 agcagttcaa attgctgttg actagcttct tcttgctgaa aagctttcac gattttctgg
    25021 ttaccgattc gttcggtcac aaagcccgag atatccccaa taatcctttg ctgtctatcg
    25081 aaatttgttt gtgaagcacg agcaacgatc caactcacaa ggaaaataat tggtgtagaa
    25141 caaagaacga ccaatgtcag aagtggactt agacgcatca taaaaataaa cgaaagaatg
    25201 atgattgcca ctccagaaaa taattgattg aaggccgcag aaattgcaat ggaaatattg
    25261 tccatatcat ttgtaaatcg actgacgata tttccgtggg agttttgatc ataataacga
    25321 agtggcaatc gattcagatg agcaaatgca tcttttctta attttgcaac ggataagtaa
    25381 gctaaccgat tgcttaacac ttggatgagc cattgactga ctacgttgat taaaacgata
    25441 ccaccgaata gaataagtaa atggaacagt cgattgaagc ggacctgtcc ttctccaatc
    25501 atttgatcga tactttgacc aatttgtaac gtcatcacaa ctgttgcaat cccattgatg
    25561 attcctagta tcacagctag ccaaatgtct tttgcatagg cagtaaggta aggaatgaat
    25621 cggcggagcg tctgcaagga taattttttt actttcataa ctattcttcc tcctgtgatg
    25681 cgacgatttc ctgatattct gctgattgag caagtaattc ttcatgggta ccctgagcag
    25741 caatcttacc ttgatccatc actaagatat gatgtgcttc ttgaatagag cggacacgtt
    25801 gggagataat aatcactgta ctgccaagat aagtctgtag cgccttacgc aaatccaagt
    25861 ctgtttgata atccaacgca cttaaagagt catctaaaat cagtaatgct ggcttggcaa
    25921 tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc agagaaattt ttcccatttt
    25981 ccatgacttt tgtatctaaa ccatttggaa gctgacggac aaattcttct gcctgagcga
    26041 ttttcagagc ttcccaacat tctttttctg tggcgtcttt cttaccccat tgaaggtttt
    26101 cacgaatagt cccggtaaat agtacagaag tttggggaac aagagcaatt tgctgacgga
    26161 gatgatccag ctgccaagaa cgaacatcca ttccgtccat tataactgta ccggaactga
    26221 catcatagaa acgcggaatc aattgtgtca aagtcgtttt cccgcttccg gttggaccaa
    26281 tgattccaag gactgtattt cgcttcagct gaaatgtaat attttggaga gaaagaccag
    26341 aatcaggagt ataacggaag tcaacatggt caaaagcgat aattgcttca gaagaaaaat
    26401 cgggagtagt actgacctca gcttcaagaa tcgaattttc tgtgtctagc acctctttca
    26461 cccgatttcc tgaagcttcg gcacgtgtga agatcacgac gaggtttgaa acaacgatca
    26521 gtgccaaaag catttgattc atataattga tcaaagcaag aacttgtcct tgttgcaagc
    26581 taccaatatt tactttgatc ccgccaactg tcagcaaaca gatgatcccg acattcatga
    26641 tcaaggttgt agcaggtgat aatagggcag agatattgga aacacgtaaa taattgttcg
    26701 ctagatcatt tgtgctttta tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc
    26761 ggatgacacg aacaccgcta agattctgac taacgtgttc attgagctga tctagttttt
    26821 cttgcacttt ttggtagaga ggaacggttt ttttgatgat aaaaaataaa atgattgaga
    26881 aaataggcaa taatgctaag aaaaacagac ctacttccca gtcgatcaca aaagccatga
    26941 ccactgaccc gatacttaaa aacggtgctc tgatcaataa acgaatgacc attgctaaag
    27001 ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat caaggtatct gttccaaaat
    27061 gattcaactc gttgtgagag aaagtattga tttttttgat tagctgatta cgtaactctg
    27121 taccaaatcc ttgtgaagct actgaagcat agtattggca aatgatagca caaatcagac
    27181 caatcacaga catagcgacc atccataagc ccatttcaat gatttttcct cggtcattca
    27241 tttttagtcc attatcgatc aaggaagcca tcattaaagg caggacaagt tcgaagacag
    27301 cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt acgatagtct tttgcataac
    27361 gtaataactg aatcatatga ttcctcctat tatttatttg tccacaattg aaaaaacaag
    27421 tactaccaca ttgtagctta ggaaatttag aatgaaaaga gaacaagcct tttcataaat
    27481 gaaagtttta ttatgaagaa gctttagaaa ttgaatagac tatttttttt aaaaaaaaga
    27541 aaatgagaaa acatgccagt ctcttcattc ataggaacgt ctattttact taatttttct
    27601 ataaatgaat tctgcataaa aaatatatca agtaaatgtt tatagatgaa actctattta
    27661 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
    27721 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
    27781 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
    27841 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
    27901 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
    27961 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
    28021 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
    28081 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
    28141 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
    28201 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
    28261 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
    28321 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
    28381 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
    28441 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
    28501 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
    28561 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
    28621 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
    28681 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
    28741 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
    28801 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
    28861 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
    28921 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
    28981 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
    29041 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
    29101 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
    29161 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
    29221 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
    29281 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
    29341 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
    29401 atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
    29461 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
    29521 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
    29581 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
    29641 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
    29701 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
    29761 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
    29821 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
    29881 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
    29941 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
    30001 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
    30061 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
    30121 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
    30181 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
    30241 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
    30301 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
    30361 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
    30421 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
    30481 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
    30541 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
    30601 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
    30661 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
    30721 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
    30781 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
    30841 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
    30901 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
    30961 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
    31021 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
    31081 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
    31141 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
    31201 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
    31261 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
    31321 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat
    31381 gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa
    31441 ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct
    31501 atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag
    31561 cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg
    31621 atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct
    31681 atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg
    31741 aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt
    31801 tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat
    31861 gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac
    31921 acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat
    31981 acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat
    32041 tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt
    32101 agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat
    32161 caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat
    32221 tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat
    32281 ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa
    32341 cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat
    32401 atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg
    32461 tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg
    32521 gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga
    32581 cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga
    32641 ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga
    32701 accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact
    32761 tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg
    32821 tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt
    32881 tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga
    32941 tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct tacttagaga
    33001 ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta
    33061 tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat
    33121 tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct
    33181 tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca
    33241 atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa
    33301 cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg
    33361 gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt
    33421 atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact
    33481 ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc
    33541 cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca
    33601 ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg
    33661 gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc
    33721 acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt
    33781 caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg
    33841 ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt
    33901 ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat
    33961 gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt
    34021 ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc
    34081 ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga
    34141 tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt
    34201 gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca
    34261 aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga
    34321 agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg
    34381 ctattctgcg actgttttac agttactact cttagggata ggaattggaa taagtaaaaa
    34441 taaaatcagc aaaaaaatag aacaagacta catacaacag ttgcgcaaaa taatcaacca
    34501 tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg
    34561 tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc
    34621 agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta
    34681 tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga
    34741 aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga
    34801 gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga
    34861 atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa
    34921 aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt
    34981 cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat
    35041 caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact
    35101 ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc
    35161 gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc
    35221 tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc
    35281 ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg
    35341 agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt
    35401 tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg
    35461 attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg
    35521 ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt
    35581 tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg
    35641 agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc
    35701 tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt
    35761 cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt
    35821 gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat
    35881 cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg
    35941 catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg
    36001 tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag agagcagcga
    36061 taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt
    36121 tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt
    36181 tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca
    36241 acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc
    36301 ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg
    36361 ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc
    36421 taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca
    36481 taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa
    36541 aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa
    36601 atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa
    36661 atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac
    36721 gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat
    36781 catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa
    36841 gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg
    36901 attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat
    36961 gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa
    37021 attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa
    37081 gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc
    37141 tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt
    37201 ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat
    37261 ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc
    37321 agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg
    37381 ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg
    37441 ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt
    37501 gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt
    37561 actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa
    37621 atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa
    37681 tagttaatcg t
//
LOCUS       DAFFFQ010000016        37154 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000016
VERSION     DAFFFQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.016"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(99..1229)
                     /locus_tag="LQB69_000938"
     CDS             complement(99..1229)
                     /locus_tag="LQB69_000938"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL3525120.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LQB69_000939"
     CDS             complement(1878..3080)
                     /locus_tag="LQB69_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3525121.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LQB69_000940"
     CDS             complement(3140..3343)
                     /locus_tag="LQB69_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL3525122.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEQLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LQB69_000941"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LQB69_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LQB69_000942"
     CDS             4880..5692
                     /locus_tag="LQB69_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525123.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="LQB69_000943"
     CDS             complement(6716..6868)
                     /locus_tag="LQB69_000943"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL3525124.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="LQB69_000944"
     CDS             complement(7059..7937)
                     /locus_tag="LQB69_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3525125.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="LQB69_000945"
     CDS             complement(7976..9094)
                     /locus_tag="LQB69_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL3525126.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="LQB69_000946"
     CDS             complement(9091..11190)
                     /locus_tag="LQB69_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL3525127.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="LQB69_000947"
     CDS             complement(11241..12062)
                     /locus_tag="LQB69_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3525128.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="LQB69_000948"
     CDS             complement(12059..12868)
                     /locus_tag="LQB69_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3525129.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="LQB69_000949"
     CDS             complement(12889..13383)
                     /locus_tag="LQB69_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3525130.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="LQB69_000950"
     CDS             complement(13395..13883)
                     /locus_tag="LQB69_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3525131.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="LQB69_000951"
     CDS             complement(13960..16656)
                     /locus_tag="LQB69_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL3525132.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="LQB69_000952"
     CDS             complement(17084..18157)
                     /locus_tag="LQB69_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3525133.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="LQB69_000953"
     CDS             complement(18154..18981)
                     /locus_tag="LQB69_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525134.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="LQB69_000954"
     CDS             complement(18982..19788)
                     /locus_tag="LQB69_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525135.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="LQB69_000955"
     CDS             complement(19800..20885)
                     /locus_tag="LQB69_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525136.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="LQB69_000956"
     CDS             complement(20902..21444)
                     /locus_tag="LQB69_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525137.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="LQB69_000957"
     CDS             21702..22691
                     /locus_tag="LQB69_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL3525138.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="LQB69_000958"
     CDS             22688..23425
                     /locus_tag="LQB69_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL3525139.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="LQB69_000959"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="LQB69_000959"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL3525140.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="LQB69_000960"
     CDS             23989..24621
                     /locus_tag="LQB69_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3525141.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="LQB69_000961"
     CDS             24706..25353
                     /locus_tag="LQB69_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3525142.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="LQB69_000962"
                     /pseudo
     CDS             25358..26341
                     /locus_tag="LQB69_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            complement(26744..28258)
                     /locus_tag="LQB69_000963"
     CDS             complement(26744..28258)
                     /locus_tag="LQB69_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL3525143.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="LQB69_000964"
     CDS             28396..29202
                     /locus_tag="LQB69_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525144.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="LQB69_000965"
     CDS             complement(29377..29946)
                     /locus_tag="LQB69_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525145.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="LQB69_000966"
     CDS             complement(30029..32089)
                     /locus_tag="LQB69_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL3525146.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="LQB69_000967"
     CDS             complement(32366..33844)
                     /locus_tag="LQB69_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525147.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LQB69_000968"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="LQB69_000968"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL3525148.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..36070)
                     /locus_tag="LQB69_000969"
     CDS             complement(35150..36070)
                     /locus_tag="LQB69_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3525149.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36183..36935
                     /gene="xth"
                     /locus_tag="LQB69_000970"
     CDS             36183..36935
                     /gene="xth"
                     /locus_tag="LQB69_000970"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL3525150.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT        11109 a         7695 c         6273 g        12077 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagctgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactag cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgagccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
    35461 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
    35521 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
    35581 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
    35641 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
    35701 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
    35761 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
    35821 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
    35881 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
    35941 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
    36001 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
    36061 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
    36121 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
    36181 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
    36241 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
    36301 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
    36361 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
    36421 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
    36481 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
    36541 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
    36601 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
    36661 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
    36721 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
    36781 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
    36841 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
    36901 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
    36961 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
    37021 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
    37081 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
    37141 ttattaccgc agat
//
LOCUS       DAFFFQ010000017        36759 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000017
VERSION     DAFFFQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.017"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            191..709
                     /locus_tag="LQB69_000971"
     CDS             191..709
                     /locus_tag="LQB69_000971"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3525151.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            837..1520
                     /locus_tag="LQB69_000972"
     CDS             837..1520
                     /locus_tag="LQB69_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL3525152.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1829..1902
                     /locus_tag="LQB69_000973"
     tRNA            1829..1902
                     /locus_tag="LQB69_000973"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Arg,seq:tct)
     gene            2061..2420
                     /locus_tag="LQB69_000974"
     CDS             2061..2420
                     /locus_tag="LQB69_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL3525153.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2543..4174
                     /locus_tag="LQB69_000975"
     CDS             2543..4174
                     /locus_tag="LQB69_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL3525154.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4236..4877)
                     /locus_tag="LQB69_000976"
     CDS             complement(4236..4877)
                     /locus_tag="LQB69_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL3525155.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5063..5139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5159..5467
                     /gene="rplU"
                     /locus_tag="LQB69_000977"
     CDS             5159..5467
                     /gene="rplU"
                     /locus_tag="LQB69_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL3525156.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5494..5838
                     /locus_tag="LQB69_000978"
     CDS             5494..5838
                     /locus_tag="LQB69_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL3525157.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5851..6144
                     /gene="rpmA"
                     /locus_tag="LQB69_000979"
     CDS             5851..6144
                     /gene="rpmA"
                     /locus_tag="LQB69_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL3525158.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6237..7385)
                     /locus_tag="LQB69_000980"
     CDS             complement(6237..7385)
                     /locus_tag="LQB69_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3525159.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPLFINYMNKVFKYAINIGLTSDNPTLN
                     LLIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLFRLLAFSGCRIGEI
                     LALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLDEKTIKQLKFWKLE
                     QRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPYIGLHGFRHTHASM
                     LYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNYINF"
     gene            complement(7397..7708)
                     /locus_tag="LQB69_000981"
     CDS             complement(7397..7708)
                     /locus_tag="LQB69_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525160.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            complement(7761..8156)
                     /locus_tag="LQB69_000982"
     CDS             complement(7761..8156)
                     /locus_tag="LQB69_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL3525161.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            complement(8192..8536)
                     /locus_tag="LQB69_000983"
     CDS             complement(8192..8536)
                     /locus_tag="LQB69_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525162.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            8837..9058
                     /locus_tag="LQB69_000984"
     CDS             8837..9058
                     /locus_tag="LQB69_000984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525163.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            9185..9961
                     /locus_tag="LQB69_000985"
     CDS             9185..9961
                     /locus_tag="LQB69_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL3525164.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            9964..10152
                     /locus_tag="LQB69_000986"
     CDS             9964..10152
                     /locus_tag="LQB69_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525165.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            10154..10438
                     /locus_tag="LQB69_000987"
     CDS             10154..10438
                     /locus_tag="LQB69_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525166.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            10459..10647
                     /locus_tag="LQB69_000988"
     CDS             10459..10647
                     /locus_tag="LQB69_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525167.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            10973..11212
                     /locus_tag="LQB69_000989"
     CDS             10973..11212
                     /locus_tag="LQB69_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525168.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            11218..11904
                     /locus_tag="LQB69_000990"
     CDS             11218..11904
                     /locus_tag="LQB69_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525169.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11907..12737
                     /locus_tag="LQB69_000991"
     CDS             11907..12737
                     /locus_tag="LQB69_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBL3525170.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            12754..13605
                     /locus_tag="LQB69_000992"
     CDS             12754..13605
                     /locus_tag="LQB69_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3525171.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13602..13961
                     /locus_tag="LQB69_000993"
     CDS             13602..13961
                     /locus_tag="LQB69_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL3525172.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            13976..14137
                     /locus_tag="LQB69_000994"
     CDS             13976..14137
                     /locus_tag="LQB69_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL3525173.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            14134..14439
                     /locus_tag="LQB69_000995"
     CDS             14134..14439
                     /locus_tag="LQB69_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525174.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            14439..14795
                     /locus_tag="LQB69_000996"
     CDS             14439..14795
                     /locus_tag="LQB69_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL3525175.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            14782..15000
                     /locus_tag="LQB69_000997"
     CDS             14782..15000
                     /locus_tag="LQB69_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525176.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            15021..15110
                     /locus_tag="LQB69_000998"
                     /pseudo
     CDS             15021..15110
                     /locus_tag="LQB69_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            15185..15652
                     /locus_tag="LQB69_000999"
     CDS             15185..15652
                     /locus_tag="LQB69_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBL3525177.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            15927..16163
                     /locus_tag="LQB69_001000"
     CDS             15927..16163
                     /locus_tag="LQB69_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525178.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            16187..16528
                     /locus_tag="LQB69_001001"
     CDS             16187..16528
                     /locus_tag="LQB69_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525179.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(16521..16712)
                     /locus_tag="LQB69_001002"
     CDS             complement(16521..16712)
                     /locus_tag="LQB69_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBL3525180.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            16922..17569
                     /locus_tag="LQB69_001003"
     CDS             16922..17569
                     /locus_tag="LQB69_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525181.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            17829..18173
                     /locus_tag="LQB69_001004"
     CDS             17829..18173
                     /locus_tag="LQB69_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL3525182.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            18178..18459
                     /locus_tag="LQB69_001005"
     CDS             18178..18459
                     /locus_tag="LQB69_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525183.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            18562..18876
                     /locus_tag="LQB69_001006"
     CDS             18562..18876
                     /locus_tag="LQB69_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL3525184.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            18854..20548
                     /locus_tag="LQB69_001007"
     CDS             18854..20548
                     /locus_tag="LQB69_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL3525185.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            20568..21746
                     /locus_tag="LQB69_001008"
     CDS             20568..21746
                     /locus_tag="LQB69_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL3525186.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            21709..22395
                     /locus_tag="LQB69_001009"
     CDS             21709..22395
                     /locus_tag="LQB69_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL3525187.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            22395..23555
                     /locus_tag="LQB69_001010"
     CDS             22395..23555
                     /locus_tag="LQB69_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL3525188.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            23565..24440
                     /locus_tag="LQB69_001011"
     CDS             23565..24440
                     /locus_tag="LQB69_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525189.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            24437..24748
                     /locus_tag="LQB69_001012"
     CDS             24437..24748
                     /locus_tag="LQB69_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525190.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            24738..25091
                     /locus_tag="LQB69_001013"
     CDS             24738..25091
                     /locus_tag="LQB69_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL3525191.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            25081..25482
                     /locus_tag="LQB69_001014"
     CDS             25081..25482
                     /locus_tag="LQB69_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525192.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            25475..25879
                     /locus_tag="LQB69_001015"
     CDS             25475..25879
                     /locus_tag="LQB69_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525193.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            25891..26496
                     /locus_tag="LQB69_001016"
     CDS             25891..26496
                     /locus_tag="LQB69_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525194.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            26516..26878
                     /locus_tag="LQB69_001017"
     CDS             26516..26878
                     /locus_tag="LQB69_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525195.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            26881..27063
                     /locus_tag="LQB69_001018"
     CDS             26881..27063
                     /locus_tag="LQB69_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525196.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            27080..30499
                     /locus_tag="LQB69_001019"
     CDS             27080..30499
                     /locus_tag="LQB69_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL3525197.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            30550..31287
                     /locus_tag="LQB69_001020"
     CDS             30550..31287
                     /locus_tag="LQB69_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525198.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            31297..33588
                     /locus_tag="LQB69_001021"
     CDS             31297..33588
                     /locus_tag="LQB69_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525199.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            33612..35702
                     /locus_tag="LQB69_001022"
     CDS             33612..35702
                     /locus_tag="LQB69_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525200.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            35719..35868
                     /locus_tag="LQB69_001023"
     CDS             35719..35868
                     /locus_tag="LQB69_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525201.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            35865..36311
                     /locus_tag="LQB69_001024"
     CDS             35865..36311
                     /locus_tag="LQB69_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525202.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            36313..36450
                     /locus_tag="LQB69_001025"
     CDS             36313..36450
                     /locus_tag="LQB69_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL3525203.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            36488..>36759
                     /locus_tag="LQB69_001026"
     CDS             36488..>36759
                     /locus_tag="LQB69_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525204.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT        12841 a         5619 c         7498 g        10801 t
ORIGIN      
        1 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
       61 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
      121 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
      181 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
      241 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
      301 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
      361 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
      421 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
      481 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
      541 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
      601 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
      661 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
      721 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
      781 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
      841 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
      901 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
      961 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
     1021 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
     1081 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
     1141 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
     1201 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
     1261 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
     1321 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
     1381 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
     1441 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
     1501 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
     1561 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
     1621 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
     1681 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
     1741 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
     1801 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
     1861 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
     1921 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
     1981 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
     2041 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
     2101 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
     2161 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
     2221 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
     2281 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
     2341 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
     2401 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
     2461 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
     2521 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
     2581 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
     2641 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
     2701 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
     2761 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
     2821 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
     2881 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
     2941 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
     3001 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
     3061 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
     3121 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
     3181 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
     3241 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
     3301 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
     3361 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
     3421 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
     3481 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
     3541 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
     3601 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
     3661 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
     3721 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
     3781 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
     3841 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
     3901 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
     3961 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
     4021 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
     4081 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
     4141 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
     4201 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
     4261 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
     4321 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
     4381 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
     4441 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
     4501 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
     4561 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
     4621 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
     4681 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
     4741 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
     4801 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
     4861 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
     4921 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
     4981 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
     5041 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
     5101 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
     5161 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
     5221 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
     5281 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
     5341 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
     5401 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
     5461 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
     5521 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
     5581 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
     5641 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
     5701 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
     5761 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
     5821 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
     5881 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
     5941 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
     6001 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
     6061 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
     6121 tatccagtag ctcaagaagc ttaataaata aactgctcta tctcttgtgt agcaagggat
     6181 ggagcttttt tgtaaattat gggatttttt ttgaccatac ttttgaccat actttcttag
     6241 aagttaatat aatttgttag tttttgtgtt gtttcttctt ttttgctgtt agtaatgtgt
     6301 gtatatgtgt ccatagtagt ttttatattt gcgtggccca ttctttcttg gacttctttg
     6361 tgatctgcgc ctgcctcata tagcattgat gcatgtgtat gtctaaaacc atgcagaccg
     6421 atataaggta agccggcacg ctcacgatat atattgtatc tttctgccac tgactgatta
     6481 attgtgaaat tattttcgtc attggtaaac aaataattag ctttagtaaa tcctaattga
     6541 aataagtact ttctttgttc gagcttccaa aattttagtt gctttatagt tttttcatct
     6601 aaataaatta ttcgatttga ttttttggtt ttaggagttt cagatatata atatttatct
     6661 gaacgagcta cagttttttt aatggccatt tcatttgttt taaaattaat attgtcccaa
     6721 gtgagtgcta atatttcgcc gattctacat ccgctgaatg ctaatagtct aaagagcgta
     6781 tagtctctgt ttttaaaata tggattctgt tcttgagata cttcatttag aaataattcc
     6841 aactgttctt ttgtatataa ttttaatttc ttttctgttt taatttgtgg ctttggaata
     6901 agtaaattta atgttggatt atcagatgtt aaaccaatat ttatagcata cttaaaaact
     6961 ttattcatat agtttatgaa aagagggtac tgctttgaag ttcctttttc actccaatca
     7021 tttactacct tttgacaata tgccgtatta attttagaaa gcttaatatt tccaaatttg
     7081 ggatatatat gtttattaaa gattatttta gtccttgaat atgaactttc ttttacagtt
     7141 tttttgtagc tttctagcca taattctgct gcttctttga aagtagtatt tgtacttgtg
     7201 ggcattcctg ttttttgtaa ttctagttct aacctagaaa gtgctgtttt tgcttctttt
     7261 tgtgttctaa atcctcgttt agtagtatat tttttctttc cagttagcgg atcaataccg
     7321 agataagttt taaagtacca tgctttttcg ccatttttct ttttatattg ttttatcatt
     7381 gccatcaata ttacccctat tcaaattcta attctgccat gctatcattg aaaaattcga
     7441 tcagtccttg ttcctctata aattcaagcc atatttcctt cccgttaatt aatggaactc
     7501 ctttttcttt acgaattgtt tgatacaagt tagtcacgac ttgtttccac atttctaata
     7561 cagctttatc tattttctta tcatcccagc gttttttttg gggtctattt ttgttttttt
     7621 caatttgata attagtagct ttgatgataa atgtttcgta tccggttgtg cttttttcta
     7681 accattcttt gaattctgta tatatcattt ttgttcacct ttctattttt gaacgtatgt
     7741 tcttttttgc ttaaaaaatt ttagagagct gcataacaat agatgcgagc ttttaataat
     7801 tctttcacaa gtggttcgaa gcgaacgctt aaattgttat cttctataaa tttcatgtaa
     7861 ttgatattgt gtggttctaa ttcagacttt gcaatatatc tatctaataa tttttcaatc
     7921 atgaagcgat cagcctcata ttccattttt gaatggagag agaatgctaa gttatataaa
     7981 aaataattat catgatgttt agaagcgtgt cctaattcat gtaataaggt tctttgtctt
     8041 ctatacttag ataaattagc ttttattact atggtattca tacaagcaac ataatggcca
     8101 tcggcatcaa ggttttcacg ctcttctacc ttaacgccga gttcattaat tatcatttca
     8161 atttgactgt ccaaatgact cacctactta tttagttatc ttttttctct aaatatgctt
     8221 caatcatagc tgatagtatt tccctatcat tatcggtgag cggttttccg tcgctactca
     8281 taacagatgc taaagcttct tccacggtta gttttctttc ctctggtgca actcccgcgt
     8341 taggatcatc tgtgcgtcct agcaaataat ctgttgatac atggaagtag tcagcaactt
     8401 tttgcaaagt ttctatttta ggactttgtg tcttccattt ataaatagag ttttttccaa
     8461 atcctattct attttctaat tcagaaatag aaatacctct ttttttacag agctctttaa
     8521 ccctttcgaa aagcatgtca aatcaacctt cctagagcgt acgataataa tttatacaaa
     8581 aagatagaaa ggtagttgac aaatctatcc aattgtctta tatttaactc gtaagctaat
     8641 ttaataagct aacaagcaac aagaaataca cctaataaaa taaacgaatt cgaggtcggc
     8701 aaactaagaa tgttaatttt tagggttttt ctaggcttat ttaactgtat ataaatataa
     8761 gacagttgga tagaaatgtc aataaaaatt agctaatttt attagcttac atatttaaag
     8821 aaaaggagga aacatcatga aacaaaataa aaaaactcct tccgcaaaag gagctttaat
     8881 aactttaaat tttaaagacc aaaagtttag tgctgttgtt gatgaaattc atccacaaga
     8941 gcatcaaaaa aattcttttg caactgacga gaaaaagact cagaaacgct ctgagaaagt
     9001 tcttcgaata attcaatttc tgttggaaaa gattcgtcat tttggaagtc tattttgaat
     9061 tctttattaa cagtcttgaa tacggtttgt ttgaataagg cgtcgacatc catattaaat
     9121 tcttccatac tattttcacc tcgctttcta ttcaaaatta tatcaaagaa aggaatgagc
     9181 aaaaatgaac acaccgcaaa tttttaactt tgaacaacat gaagtaagaa cagtaacaat
     9241 acatgatgaa ccattttttg ttggaaaaga tgtggctaaa gttctagggt accaaaatgg
     9301 tagtagagat attaacagac acgtcgatgt agaagatcgg caaaactacc aaaacggtac
     9361 ttttgaatcg cctagagggt taactattat taacgaatca ggtttgtact ccttaatttt
     9421 aggaagcaaa caaccaaatg ctaagaaatt taaacgttgg gtgactagcg aagtgttgcc
     9481 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
     9541 tccagataca atcatccaat tagcaactaa gttgaaagaa gaacgtactg gaagactaat
     9601 cgcagaacaa aaaattgcag aatacgaacc caaaatttcg tatttggata gtatcttatc
     9661 ttctacagat tcagtaacta ttagtcaaat cgcggctgat tatgggatgt ctccacaaca
     9721 gatgaacaaa ctacttcata agctaggtat tcagaaaaaa gtaggcaacc aatggttatt
     9781 atgcaaaaaa cacatgagac agggttacac aaaatctcat acaactgaga ttccgaaatc
     9841 tgatggcgga acaaaagttg tgatgaatac aaaatggact caaaaagggc gtctgttcat
     9901 ttatgagtcg ctgaaaaaag aaggatatat tccagaaatc gatttattag aggaggaatg
     9961 acgatgtcac aagaatttat tttgaaagtc agaattcaat tagcaaaata cggaaagtct
    10021 cagaactggt tagctgatac tatcggaatt tctagaccat atatgtcgga cattatgaat
    10081 gggcgtagaa aaccagataa acaaatcaag ccaattgagg cagcactagc agagttagag
    10141 aaggagaaat aacatgcaaa taacaattcc agataattta gtagtttccg aattaactac
    10201 acagattacg aatgctgttc ttaattcatt ggacgaacga ttacacctta tgaacaaatc
    10261 agtagagctt cctccatatc caaacaaatc agaggtaaaa aaagttttag gcattggtga
    10321 tgacaaatta acacattgga taagcctagg cttaaaaaca cagcagtgga gcaagttaga
    10381 catcagaatt gaacgatcgg aactccaaag atttttgaaa gaaaactttg agttctaaag
    10441 gcaaaggaga atgattttat gtcctacaca ttgcaacaag aacatcaaat tctaggtctg
    10501 attaaacagc gtagaaaaca attacaagat gatcgtgcag cgcttagaaa atccgatgag
    10561 ctatcagata gacaagctga actaattgct tctgaacttg aggatttgag aatgctagaa
    10621 ataaaaaata gggagattag attatgaaga agacagacac actttttata ggattcattt
    10681 tggggttatt agtgattgta gcgcaccaaa gtattatcgg aggaagcttg ttcgcagcat
    10741 tgatggtttt aatcaatctg cttgactcaa aagaaaggag caactatggc acgagaagaa
    10801 gcgctaaaaa tcggtaaagt gattgctgat aattggtggg caaatagccg ttctattatt
    10861 ttaagcaagc aacatatcga aaagaaaaaa gcatggcaac aaataaaaaa gtgactccgc
    10921 cgaccaaagc aatgagtcac tcaaaaaatc ataactaagg agattttaac atatggaaaa
    10981 tgaactttcc actctagatc aatatttggc cgatcctgat tggggcaaat cgaatttaca
    11041 actggttgct aacaagtacg aaaaatcaat tgatgaagca attgttgcat tgactgatgc
    11101 agcttccata accgctgatg acagtaaatg gaccaagaga gacttgggca ttctaaaacg
    11161 aggcgttaat tggcttgaag atcagtacag agaagaaaca aaagagaagt gatatgagtg
    11221 tttaaaccac taatagattc atactcggcg gtactaaaga aatttaaagg taacgacatt
    11281 ggtgcaacaa tcaacgaaga agtaaatatc gaacggctga aaacgatgta tgacggatat
    11341 gatggcgatc gagtcattga aattcgcttt attgatccac gacggttcac agtacagcaa
    11401 agaaacttca tctatgcgct cataggcgat attttcatcg atacaggcat gccaacggac
    11461 ttctggaagg aattcttcta cttccgtttt gaaggtgtca cagggcgcga aataagcctc
    11521 aaagacgaat cgaatacaac cgtgagtgat gccaatatct tagcgaatat catcctagat
    11581 ttcatctttg aacatcatat tcctttcaaa gaaggatatg agattttacc agcgaatcaa
    11641 gagtattact tctacaaatg cattacaaaa agagtctgct gcatctgtgg caaaacagga
    11701 gctgatatcg atcactttga caaagcgctg ggaagacgaa agcgcaaaga agttgatcat
    11761 tcagagtaca catttgcagc actctgcaga atccatcaca cagagaaaca caaaataggt
    11821 gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca ggaaacaatt
    11881 aaaaagttaa ggataggagg ataaatttgg ctgagataag ttggatcaaa cttaaaacta
    11941 ctatgtttga cgatgaaaaa atacgattaa tccaagctgt tcctgagtcg gatgccatca
    12001 tcgttatatg gattcgatta ctagttttag caggaaagac taacgacgat ggtctgatat
    12061 atatccagag gaacatgcct tataccgaag aaatgcttgc tacattgttt ggcaaaaacg
    12121 taaatacggt tcgcttagcg ctaactacat tggcaaattt caacatgatt gatctaagca
    12181 gtgatggact aattgccatc agtaattggg aaaaacatca aaatatcgag ggtatggata
    12241 aagtaaggct aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag agacaggaac
    12301 gtctcaaatt ggaaaatgac gttagcgtga cgtcacgtga cggtacagat aaagatatag
    12361 aagaagataa agatatagat aaagaagaaa agaaaggtaa gtattctgac gaacacttac
    12421 gccttgctaa aaagttgcaa agtaatttaa ctgaagattt tccaaaagaa atgaacaaag
    12481 tagatatcga aaaatgggca gacacaatca ggttgatgga agaaagagat aaagcatcta
    12541 tagaagcgat tgagtatgtg atcaattggc tacctacaaa tgaattttgg tttggaaata
    12601 ttagaagtgc taagaaattg agagaaaaat ttgagaagct caaattcgaa atcaaagcag
    12661 acaagaagaa tcataaaaag caaagtcaaa aactacagta cagcaatcct agtgaatatg
    12721 acgacttgcc aatttaaaaa ggagatgcat cacatggaaa gcctagcaaa tgctatggag
    12781 aaactaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg
    12841 tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac atgcatgagt
    12901 tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat atataacgat
    12961 agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac agataaaact
    13021 ttgtttaaat gcaaaatgga aaattatcac gtagtggatc aagaaacaaa aatcgcttta
    13081 gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc atcctgcaca tttcattcta
    13141 tcagggaaat caggaagcgg aaaaagccac ttatcaatgg ccacagcttg ggaaatactt
    13201 gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagaatt attagagcaa
    13261 ataaagtttt cttataacaa tactgaactg agaaaagaaa ttgaaggatc gcttatagcc
    13321 gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt aggtagtggg
    13381 gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga agccagacag
    13441 aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga agcctacggt
    13501 gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa attccaacaa
    13561 acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa taccgtaata
    13621 aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag tattacgatc
    13681 tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa acttcaggaa agatttgagc
    13741 tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat attcccgact
    13801 tcacatttta taaaaacggc aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa
    13861 aagactttaa gatcaaggca aagttgttct gtcatcaata tcaagcgccg ttgatattag
    13921 ctaaaaaata tcggaatacg ttcaaggaag agcgttttta acgaggtggt ccatcatgac
    13981 aacagaagaa gtgattcaaa tgcgtattcg aaacattcag cgtgaaattg acgatctaga
    14041 acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga
    14101 gaacttaaga agggaaattc gtagattgga ggaatgagcg tggataagaa agcgacaatg
    14161 aaacgaattg ctgaattaac caagtcagaa tcttggcagg aagacaaaga aatagttgca
    14221 gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga
    14281 aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtacagctga acagttatct
    14341 gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt atggattgat
    14401 tcaaaaggac gacagtttag gtatgtggag gagagataat ggatctcatt acacaataca
    14461 gtgacatcat cctcaagaaa atcatgatga agattcagaa agacaaaaaa tcaaaagaac
    14521 gagctgaatt agttaagtta gaaatggctg aaacaggagc aggagtgcga agtagcaggc
    14581 attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa gggttcgatc
    14641 agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa gatcgtttca
    14701 aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca gcaaatgata
    14761 aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    14821 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    14881 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    14941 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    15001 taccgaagtt tagagcgtgg gatagagaaa aagacagaat gaaggcagta gctttggttg
    15061 aatttgctga ggcgtataaa tcaggagaac tcatttcacg tacaaaataa aaaaagtcgg
    15121 attcctccga ctatgaataa tatttccgac ataagtatta tatcataatt gggggaatca
    15181 gaggatggta cttttcgatg taaagaaata tgagacacca agcgcgaaag atgttgatat
    15241 ggaacgcaca aaacataatg tcgctgtgtt tctttcagca tatctatcag ctagatgtag
    15301 agtagggcaa cctcgagaac caaaagtgac agcatcttat tccttggttc caccttctac
    15361 agctgatcat atatttgaag cagaaagaat gctgatcgat aaagaagaag cacaggaaga
    15421 atttgagtat ctgcataaat tgtttgttcg aggctattca gcgatacagc atccgcataa
    15481 gcctgatgtg actgaaaggc gcaagaagat attctatgat cgttatatca atggtctgcc
    15541 catttatgta actgctcaaa ggaataatac tagcgaagaa tcggttaaag tagaatcaaa
    15601 cagaattatc atccaatttg cttcatcgtt agaactggtt gctttcaagt agccagtttt
    15661 tacacttttt ataccctttt attaccaatt tggtttccat tttatacctt ttttatacca
    15721 atcacttacc tattcaacgt tgtattatga tagtgtcgaa agattaggaa acaggatcga
    15781 caaaataaaa tgtaagggag gaaatctccc tcatcgttta attaagcttc gataggcagc
    15841 agcggaaata ttaagaataa ggatgtgaat ttcaactcct tctaaattgt tcttattatc
    15901 tatcatccgt tgctgtctat taatttatgt attggagggt atataaaatg aagatcgctt
    15961 ccgaaaaaat tgatcaacta acaattttag taaaccaaag aatcgaagat attatcaaag
    16021 aattcgaagg tgaaaagatt aagaacataa agcttacttt aatggatcat aagttcgaat
    16081 taaattcgac aaaaaggaag atcgttgagc ttatagaaga agaaatagct ctccagatca
    16141 atgagcaaca attagctaat taatttatgt attggagggg aaatgaatgg aagtcgaaca
    16201 aataaagtat caaggtatgg atcgggaacg gttgattgat attattgagc aacaacgttt
    16261 agaattgatc caaaaagaag ctgtatgtaa agagtacaaa aaacatttgg aacaagtaat
    16321 tgagtatcat tcagtagaaa agtacagatc agtggtgcaa aaaaatagag caacgagtcc
    16381 tgattctcgt caagatgtat cgattagtat caagacgcca gtatttaatt ctgttaaatc
    16441 agaaaagcta gaaagtatct caactgcttt ggtagaaaaa gcagctgaaa tgaactccct
    16501 agcaaatgaa gttagtcgtc ttatttagtc atatcgataa cttgagagtc tttggtgatt
    16561 ccgtttttta tagaattaat ggtttgttca gcggaatatt tagtcaagta tgtttcgcta
    16621 gttgctacta cttcattgtt attggactta ataacgaagt aatattggcc attagttgct
    16681 tctcttataa caaagtacaa atttttcacc acctataatt tatttcagcg gaccactcgc
    16741 tgataattaa aattataagc tatgtataaa atttcacaat atcaatttgt cgctgtggcg
    16801 gaaagggtag acgcaataaa ggatgaatgg gaaaagtcaa tgagtaatta tctgaaattt
    16861 acaagtcccc tgtttagtta tctaagatta tttgataata ttatttctga ggtgatagaa
    16921 aatggtgggc gtagttaaat ttcttgggtt tacatttgat ggatatggta cgttaggcga
    16981 atgggtaggt attattttga tagtgattac aattcaacaa acatcaaagt actactggta
    17041 ttctaatagt tctaaaattg tatcaagaat aactgaagga ataaccaaaa aagatattac
    17101 tgtcgtcggt aaatatgggt ttactataac tttattaaat gaagggaaat ctgaaaccgc
    17161 aataaagttt tgcgggatta taggtaaata taacattttc caacgtttta tcagaaagca
    17221 ttggtacaaa tatatttaca aataccctgt agttaaaaag cttattgaaa aatatgtgga
    17281 ccacgaaata tcttatctca atccaacaga ccttattaat caagaattcc agaatattgg
    17341 atacttagaa aaaggtctaa aaataccaat tgtaaatgaa agtttagtta ctgcaatggt
    17401 agctaacata aagaacagca agaaattaga aaagagatat aaaaaatgta aaaaattgaa
    17461 attttccttt ttattcttag aatataatgg caaagaatat tatcaagata tttatgtttt
    17521 gcgaggctcg aacttatata aagacattaa aaataagatt tcagtatgag attttttaaa
    17581 attcgtagat cactctttga gtggtctttt tattttgcgt aaaggaggca cgacgatgta
    17641 tagaccacaa tacttagaac agaagcatga agtaatcact gtgcaaaacg gtaacggtga
    17701 gatagtacga aagtatagaa gaccaataaa gagcgataca tataaacgga aggaaagcaa
    17761 tgaagttatt ccattgtatg gcaaaagaat agctaagtat taaataagat tgcgaaagga
    17821 gatgggacat gaccgaggaa ttctatagat ggttattaca gttgataaga gaagatcgtt
    17881 tggttaagtt ctatcagtct cctaaatggc gcaggcttag agagaaagcg atgaaacgag
    17941 atcactatga atgccaagag tgtagaagac taggtaagta tcatagagta gagaacgttc
    18001 atcatataaa ggaagtcaag gatagacctg acttagcttt agatttagat aatcttattt
    18061 gtttatgtgt tgaacatcat aatgaagttc atggcagata tcttacagcg ttagataaac
    18121 aagagaagaa gatagaaagc ttcgctaact tcgatgcaag tgaaaggtgg taagtgcatg
    18181 atcatcaatg acaatggcag agagtatgat acagaaaaga ttgaagagta ttcatcttat
    18241 actcagggat taattaaacg tttgatatac gttcgctatg taggtattag ggatctgtta
    18301 tcagataact gttgtagtaa atacaaagtg aatcaagtaa gagaagcgtt gaataaagat
    18361 aataacgtcg aaagaataaa aaatgttttt ggatatagta ttgaagagat taattattac
    18421 attgacttcg ctgaagcttt cattccgatg gtgagataac ccccccttaa aataaatcgc
    18481 aaattttttg ggggtgatga aacggagggg gctgtcagga aaagagattt tttcgaactt
    18541 tatcatgaaa ggagggctaa aatgtttaaa aacgaattgt ctcaaaatcg ctacagagaa
    18601 aaattacgcc gctctttaat aagccaattg gaaagtcaga aaacaaatat tgagccattc
    18661 ttagataatg ttgatcgtta tatcagttta tgggaaacgg cgatatcact ggaagaagat
    18721 atatccgaga acggcattag actggagaat ggtaaaaaga atgaatcagt agcgttgctt
    18781 gtttctgtca acaaacaaat gggattgatg ttggataaac ttgccattac tcctgaattg
    18841 gtaggtgaag caaatgaatc aattcctgag ttataagcat attgaaaatt ggttcaaagc
    18901 tatagaagaa ggcactatca aggtatgcaa agagcaatta ttgctaaaaa attatctaga
    18961 agaaagagtc tttactagag aagatattta cttcgataag cagatggtag aggattcaat
    19021 caatatacca gcacaatact ttccattcga attaattccg tgggaaaaat ttctacaatg
    19081 ttttatttat ggtgttcgat ggaaaaaaga taaaacacta gtgttcaata gatatctttc
    19141 attaatggga cgtggtaatg gtaaaactgg ttttgcttct tggaacaact tctttctact
    19201 aaccgctaaa cacggtatta aaaattatga tattgatatc tatgccaata atgaaagcca
    19261 agcaaagact agttttgatg atgtatttaa agtaattaaa gatcatcctg atttagataa
    19321 aaaagtattt aaagctacga aggaagttat tcaaaatatc gctacaaaca gcaaacttcg
    19381 ttataacacg gcaaatgcta gaacaaaaga tgggaagcga ccaggtgcaa accgctttga
    19441 tgaaattcac gaaaatgaag attattcaat gataaatgtg gctacttctg gtggtggtaa
    19501 aattcgagat tatagagaat tttatgatac aactaatggt catgttcgtg gtggtccgct
    19561 tgatgacatt atagaagaat caaaaatgat tctttctgga gaacttggaa ttgacaagga
    19621 tggagcagaa ttttctagtt tgtttccatt tatttgtcgc ttggataacg ataatgaagt
    19681 tgatgatccc gacatgtggg aaaaagcttg tccaactatt aattacaatg cagatttaaa
    19741 acggaaaatg tttcaagaat actctcaaat gcaacgtaat gctggtttaa gacttacgtt
    19801 catgaccaaa cgaatgaaca gacctatgga agatacacga tttgctgttg cttcatatga
    19861 tgatgttctg catacgaaag aaaaagaatt tcctgaaaaa atggatgaag tgataggaac
    19921 agtcgatttt gctgatagac gagattttgc cagcgttggg ttgctaggaa aatacgataa
    19981 agatgtttat tttacacaac atacttttat ccacgaatca gcccttcgat tacaaaacat
    20041 caaacgagag gttatagata tttctataga tcaaggaaaa tcacagatcg ttcatggaaa
    20101 aaatatagaa gctgattata ttgtaggttg gtttcttgaa atgagtaata aatattatat
    20161 taaaaaaatc gctatggata tgtaccgtgc aaaaatattg aagcccgctt tagaagaagc
    20221 aggttttact gtggaaattg ttcgaagcgg atctgttaca catggtatgt taaaagatct
    20281 ggttgatgac ctttttatta atcaacgttt attttttggt gacgatgcga ttatgcgttg
    20341 gtattgcatg aatgtatatg aagagcatat ttctaatgga aatatacgct atgaaaaaat
    20401 agaacctgaa actagaaaaa cggatggctt tttttcattc cttcatggtt tgaatttttt
    20461 agatgatatt tatgattctg ctcctgtaac agtcacaaat agctcagtag aaaatacagg
    20521 aactggattt actcctctag tattctaact tgaaaggagg tgagaaagtg gggatttttc
    20581 aaaaggcggt aggatacttc acaaaaaaag caacggttcc tttagaagaa tacttttgta
    20641 aattgcaagt tgattttgtg tatcgaaaat ttgcgattga aacttgtatt gatttgattg
    20701 caaatgcgat gagtaaagcg gaattcaagt catatgaaga tggaaaaaat aaaaagaatg
    20761 atctttacta taggctgaat gtagctccta ataagaaaaa taatgcaaca gaatttagaa
    20821 aaaaactgat caggagatta atattctaca atgaagtatt gatcgtttct ccgtctaata
    20881 attctagcga aatatttatt gcggatagtt gggatgtcac agaatatgca ttgaaagatg
    20941 atgtgttttc tcaagtgcaa attaacaaca tagtccttga tagagaattt ctagaaagtg
    21001 atgttatcta tataaaatac gcagatcaac aaattaggca actagtcgat gcgtattatc
    21061 aagcgtatgg gaaactcatt tctagtgcca tgaatgttta caagcgctct aacgctcgta
    21121 gatacgtact gaaagggaat ttattccgac cgcaagacaa tacaacacaa gatcaaatca
    21181 ataaaatgat gacatcacaa tttaaggctt ttatggaagc tgataatgca ggtgcggtat
    21241 ttcaattaca aaatgagtac acattagaag atttcagcgg aaacttccaa agcaattcaa
    21301 gagatataaa aaacttaata gacgacatct ttgagatgac agcagcagcg tttcacgttc
    21361 cgaaaaacct actaaaggga gacatgagtg ggttatcgga tcaagtggac gcttttttaa
    21421 tgttcgaaat cataccgatt gctgaactta ttcaggatgc gtttaacgct agtctctatg
    21481 aagtagaaga atacttgtca gggaattttg tacgtgtgga tacaactatg atcaagatta
    21541 ctagcttcaa agatttggtt gacgctattg atgtaggcat tagaaatgga gtatttacaa
    21601 tcaacgaagg aagagagcgc gttggaaatg atcgctctga taaggcgatg gcagatgaaa
    21661 tatttataac taaaaacaat caacaagtat cgaaaggagg tgaggcgaat gacgacaatg
    21721 aaaacatttc tagcagtaaa gaatgaaggc gcagtaccgc aaatttttat tcagggattt
    21781 attggttcta gttggttctt tgaagggaat actgacaagg gaatcaaaaa tattttggat
    21841 agtctaggtg atcaagaaga aattgaagta gtaattaatt caaacggtgg agacgtattt
    21901 caagggattg ctattgggaa cttacttaag tcaaataaag caaaagttaa cgttgtgatt
    21961 aacggcttag ccgctagtgc tgcttcaatt atcgcaatgg ctggcgatac tataaaaatc
    22021 tacaacaatg cacaattgat gattcaccgc gcttccacat acggagaagg aaatgtcgat
    22081 gacttccgta cgattgctga ccaactggaa tcaattgata aatcggtaaa ggcttcatat
    22141 aaaacacgat tcaatggcac agatgaagca ttgcaagaac ttcttgaaaa agaatcgttt
    22201 atggatgcag aaacagcttt gagttatgga ttggtcgatg aaattatcga tgcagaaaat
    22261 agctcaggta ctgaagctaa aaaagaacaa agcgttgaag aaattttgaa tgacgttaaa
    22321 gaaaaaagag cagaaaaaat tgctgcattt acagcagcat taaataaaac atttggacaa
    22381 ggagatgtaa aataatgaca gttaaaaatt taaaaggtgt aacagctgca agcgaccaat
    22441 tgatgaaagc ttttaaagat ggtaacgaag aatcttttag cgcagctatg gtaagcttat
    22501 ctaaggaaat tcaggataaa attttagaag aagcaacagc aaaaaatcaa gatcaattag
    22561 tattaatgaa ccgtggtcag cgtgtattaa ctacacaaga aacaaaattc tataacgaag
    22621 tagtgaaaaa cgaaggtttt gcaggggtcg aagaattagt gccagctact gtatttgaac
    22681 gtgtatttga agatttagaa caatctcatc cactattgca aaaaattact tttgttaaca
    22741 caactggtgt aacagaatgg attgtgtcac gtggagtcaa tccagcatgg tggggtaaac
    22801 tttgcgaagc tgttaaaaaa gttttagata atggctttga tgtaattaac atgaagcagt
    22861 tcaagctatc aggttatatt cctgtatgta aggcaatgct tgatttaggt ccagtatggt
    22921 tagatcgtta tgtccgtact gttttagtag aatcattgag aattgcatta gaacaagcaa
    22981 ttgttgatgg tactggtaaa gatatgccag tcggaatgat gcgtgacatg agcaaacaaa
    23041 ctagcggaga atatgctgaa aaaaaagcag aacctattac agctttagat gctgtaacta
    23101 tgggcggttt gatggcgcga ctatcaaaat tcaatatcga aggtgtgaat gatccgattt
    23161 atcgtaatgt gaatccttct gatgtggtcc taattgtgaa tccaactgat tactggtcta
    23221 aagtattccc agctaagact gtactaactg ctaatggaga atacgtacaa gtattgccag
    23281 taccagtttc agatttgcag tcaacggctg tgccagaagg aaaagcagtt attggggtag
    23341 cctcagatta cttcatgggt gtaggatcta cactaaaaat tgaagcttca gatgaatacc
    23401 attttgttga agacgaacgc atttatctag ctaaacaata tgcaaacggt caacctaaac
    23461 gtaacgatag tttcattgta ttagatatta gcgctttggg aactactact acaactacaa
    23521 aaccaacaac cacaacaact acaacacaag cgtaggtgat cagaatgaag tatattcttt
    23581 gtcagccggc aatcaatcgg tttaaatggg agcttgaagt ttgtttaact aatctgaaga
    23641 aactaggaat caaagatatc gtattgcttt tcagcagaca cgatgatcag attcctattt
    23701 tttttgagaa ggaatatggt gttgaagttc atgtgtacga tgatctgcgg gacgacaaag
    23761 agtatattcc ttcgattaaa ccatatttat ggtggaaata tttagaagaa gatcattcgc
    23821 gtgaggacga ccgatatttc tatatcgatt cggatgtcat tttcaataaa agaattaatt
    23881 tgcgcaaatt gccttctaaa gatgatgttt ggtattgtag cgactgctgt agttatctaa
    23941 gtcttgatta tattagaagc tgtgaaaacg gagaaaatat tctaaaagat atggcaaaca
    24001 ttgtaaatgt tacagtagaa tctttggaaa ctataaacac taattcagga ggcgcacagt
    24061 gggttattaa ccgccctaaa gcgaattatt ggaaaaaggt ttatctggat tctaatcggc
    24121 tatatcgcta ccttagaggg caaaaaacaa atatacaaat ctggacagcc gagatgtggg
    24181 cacagctttg gaacatgatg tatttcaata ttggtcctaa agttcacgag gaattagact
    24241 tttgttttgc tactgatcca atagaaaaag ttaaagaagt aaaaatctta cacaatgctg
    24301 gagtaacaac aaacgatgaa gatttatttt tcaaagggag atacgtgact tctacgcctt
    24361 ttgatgaaga tttatcattt gtaaacaaga aaaaatgctc ttacgcatat gttaaagcaa
    24421 ttaaggcggt ggttagatga cgcctgaaca agtgactgaa gaattgctaa tagctgtgaa
    24481 ggataatatt tacgttacct ggaacgaaga agatgagtca attaaaaaga tgatagctaa
    24541 aaatgctgtt tatcttcaaa gtaaagtgag tacaacactt tctttttctc ctgaaagctt
    24601 agaatacgga ttgctaatcg aaagatgtag atacgactgg aatcgtgctt tagatgagtt
    24661 tgaacaaaat ttcgctagtg agttattagg tttcattcaa cattatgcgc tacaagaata
    24721 tattgcaggt gatgtgaatg gcgaataatc gtagactcga agaaacattc aacgatggtt
    24781 ggttaaagat tttgacgcaa actaccaaaa gaaatgaact aggaaaaaag attggtgtag
    24841 aagatacaga aatcacttct ttaaaattta gaaatctttc catgagagat agtgatataa
    24901 cagctatgga tgcgatggga tcgaaattaa ctaagaaagt aaagactcca tttcatccaa
    24961 tcgccaagaa atttaataaa gatcaatatt ttatcgtaat cgatagtatg cgttacaacg
    25021 ttatctatgc cgattacgat aatttttata tctattttta tcttgaaagt gtgggtgaat
    25081 atggtgattg ataattctaa agaaaaagaa cgtttaaata agcaaatttc tgctatcaaa
    25141 acttccttag aagagcattt tggcctcaaa ctctttcaag actccgtagg cgaggatgag
    25201 ctacctgatg attttaatta cttcattctc gaaacaggag aaatagaaat gatcactgag
    25261 ccaaaatata gcgtgggtca aaatctatat ctaactttct attcagaaaa tagagaagat
    25321 ttaacaggag attcactaga tattatttca ttgattcaaa atcgttcgat tcgttttcag
    25381 agaatggatc ccaatcattt aaaactagag aaccaagatc gctatatcga tcaattggta
    25441 tttacgttta gacgattatt gaagagtgat tgtcatggct aaaaatagtt gggagctaaa
    25501 aataaatgga catgatgaac ttcttgtgcg gatggaacgc tattcaagcg agagcgaacg
    25561 actgattaac gaagcattga aatcaaaagg ttcggctatt gcagtggata ggattacaga
    25621 aaaaattcct gtttctgaag cagatttaag aagagggcac caacacgcaa aaaatagtcg
    25681 tccacttaag actcaataca ttaatttggg tttcatcatt agacctacaa gaaaatttga
    25741 gtatttaaaa tatcctgatt tggggatagg tacttctaaa agaaatcagc cggacgaatt
    25801 tatgagaaga ggattaggtc ttgcacttga tccaattaca gaacttctga ttcgtcaatt
    25861 cgataaatta aataaatagg aggaacaaca atggctaaaa caacaacagt aacaactttt
    25921 gacaatataa gtattaaaag aatttctttt aattttaaga atgcaacaaa tgcaatttca
    25981 actgattgta atggacaatt agatggcgaa acagaaatgc agagaattgt aaaaaaatgc
    26041 ggttcaacag aagtaaaatc gaaatctaaa ccaatcaata tgacggtaac aattactgca
    26101 catgtaccga tggaagttta tcgacgcttc aatgggttga aacaagatga acgtattaaa
    26161 tcgggaattt actcttatgg tcctgattcc gtaggggaag atttctctct tgctgcagag
    26221 atcgtggatg atttcgaaga aaaaaataag ctgattggta tgttagcatg cacttcgaat
    26281 acaggattaa cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa
    26341 acaaaagtta tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt
    26401 gaagaagact taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact
    26461 tcagttgtga caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa
    26521 cgaagattac tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact
    26581 gaatatcaag aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat
    26641 gactgtggga aaaggatcca tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat
    26701 tgcttaccgc caagcgaaca tgactgatta tatttcattc gatgaattta tcgataaatg
    26761 ggattttgat atggctactg ccagctatat ttatcaattg atgatgttca aacaagcacg
    26821 cgatgcttat caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa
    26881 atgccaaagc tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt
    26941 tctatgcctt cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct
    27001 tacaacaaga gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac
    27061 taaagaaagg aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa cattcatagc
    27121 taacgatgac ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag
    27181 agcagaatta aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt
    27241 aggaagtaaa ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt
    27301 aactagccaa cgtttagcca atgccaaaaa atattatgga gaaaattcca ccgaagttca
    27361 gaaacttgag agagaactga ttaaccaaca aacagcgcaa caacgtttgt caaacgaaat
    27421 tgataaaacg agtaatgcac tagctcaagc aaaaggcgaa atacagacgt acgagtctac
    27481 aatgcaacag ttggatagtg aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc
    27541 cgaatacaaa aaatggcaag caactgctgg tcaatcagct tctgaatccg agaaattagc
    27601 gaaagcccaa gaatatgttt ctcaacaatc tgaaaatgca gagaaaacga tagatatcct
    27661 gagacgacag ttagaagcta cacagtctga gtttggcgct acatccacag aagcaatgca
    27721 gatggaggcg aagcttaatg atgctgaacg tgaatttgaa gagttaggac aagctgctaa
    27781 aaatgtagat acaactaact tggacgatat cggaagcaaa atagatatga ataatctaat
    27841 ggaagcttct gacgttttaa gcgacattgg cgataagctt acagaattag ggaaacaagc
    27901 agtggactct gctaatagtg taggtagttc ccagagtaaa atacaagcta attttggttt
    27961 gactaaacaa gaggctgaag aattaacgaa tgtagccaga gacatttatt ataaaggttt
    28021 tggagaatcg ttagatcagt ccacagatgc attgattttg gtaaagcgta atttaggcga
    28081 tttaaataat caagatttac aaaatatcac ggaacaagct atggtcctag aaaacaccat
    28141 gggcgctgat atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca atttcggctt
    28201 gagcgctcaa gatgcaatgg atttaatggt ttcgggtact caaaacggtt tagataaaac
    28261 gcacgaatta ggcgacaata tggcagaata tagccaatta tggagtcaaa tgggatattc
    28321 agctgatgaa acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt ataaccttga
    28381 taaagtcaat gacttagtta aggaaatggg aatatcgtta acagatggtc gatttgagca
    28441 aaacatggat atgtttagtg aaagtactag aaaagctttt gaagagtgga aaaatggcgg
    28501 aggaacacaa aaagacgtta ttaattccat gattcaagat tttagcaata tggatggtca
    28561 atacgaccaa ttaaataaag cttctacaat ttggtctgca cttggcgaag ataacgcgat
    28621 gaaagttgtc caatctttga ctgatgttaa ccatacattt gatgatgtta gtggatctgc
    28681 acaaaaaatg aatgaagatt ctactactcc gttacaagaa ttaaacggaa aaatagctga
    28741 attaaaggat tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat
    28801 tgattttcta ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggatta
    28861 tgccgtagcg attggcggat tgactgctgc atttacttta ttaatgccaa taatagttgg
    28921 cttcatggct ctaggtggtc ctactacatt aataatagga gcagttatta ctgttattgc
    28981 tggagttata gcaattataa aaaactgggg cgcaattact gactggttta agggaatatg
    29041 gagtaaattc actgattggt tgggtggtac ttgggaaagt ataaaagaag gtgcctcatc
    29101 agtttgggat ggagttaaag aaacctggtc tggatttgta gattgggttc aagatatttg
    29161 gcaaggagtt tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa
    29221 catctggcaa ggagtccaag agacttggca agcattcgtt gattgggttt caaatatttg
    29281 gaacggagtc aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat
    29341 accatggacc ttaataacgt cattgattca agccggtatt aatattatcg tgggtatttt
    29401 tgatgtagct ggacagttat taggcgcagc ttggcaagct gtttggacac ctatttctga
    29461 tttccttaaa aacacttggg atactatgac acaatgggta agcatcgctt ggaatggaat
    29521 tgtaactaca ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac
    29581 agctattagt actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat
    29641 ttggaacagt atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca
    29701 aaatatttgg tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac
    29761 agcatcaaat atctggaata gcgtgactag tagtataagc aatgctataa acgcagctaa
    29821 aagtgccatt caaagtgttt ggaatagtat atcttcgtgg atcagcggaa tttggaacgg
    29881 tatcaaaaac actgctttga atctttggaa tggaattaca agcactatta gctctaaagt
    29941 aaacgatgga aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga
    30001 catattcaat aatgttaaaa gtacaattgc taacatttgg gaaggtatca aaaagactgt
    30061 tagcgctcca attgattgga tcagagataa aatcagtagt atctttgata atttgaatat
    30121 ttctatacca catattccgt taccacattt taaattgagc ggagaattca atccattgaa
    30181 ggggaaaatc ccaacgttgg gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc
    30241 gaatattatc ggtgtcggcg aagcaggacc tgaagcagtt ttacctttga aaagatctgt
    30301 gctgcaagaa attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac
    30361 gattcaacct gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt
    30421 tactatgaag caaacaactc aacaaatcat tgatagcatt acaaaagttc aaaatgataa
    30481 tgcttcggca tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga
    30541 tgtgaaaaaa tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat
    30601 aaattggctt tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact
    30661 tctgttttag gaaggttagg tggcttagta caagattact cgtttgaaga catgtcgttt
    30721 acattgcact ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt
    30781 tatatcatgc gtcattggtt aaattatgca aagaaattag aattctctga tgatcccaac
    30841 gtctattatg ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg
    30901 ggagagttcg atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct
    30961 ataaccgtag ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt
    31021 ccaaagatta tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg
    31081 tttagttttg aaggcttagt agtaggaact gacgtagtca ttgatagtga tttgatgctt
    31141 tgctacgaag agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg
    31201 caatatccga cattgcaagt ggatattaat tattttaatt gtactggttt gagcaaaata
    31261 caaatttatc gtaatgggtt aaggtaggtg aaatagatga tcgataattt aataactatt
    31321 tacgataaaa acgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc
    31381 gctttgtcag actggttaac agctactgtt agcaataaat taaacggagc cgagatattt
    31441 cagggtactt atccaataag cggaactaat gcagatttga ttgtagaagg acgtattatt
    31501 cagtgttatg tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaagact
    31561 tctgtaatag gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa
    31621 tcggtgttga ataaatatga cagtggaaca gaaaagatca ctgctagtca ggcatggcaa
    31681 aacgcaaaaa ctttagcgaa accagttatt ccttcacagt tttctttctc gtcattagta
    31741 gatacgcttg ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa
    31801 gagggatcta ttctagatcg atttcatggg gaatttctaa aagataataa cacattacgt
    31861 catgaaaaaa ggctaggcac ggatcataaa atcaaagcga tttatactaa aaacttaact
    31921 ggtcttgact tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc
    31981 agctcttcag aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac
    32041 gtggatgatt atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat
    32101 gtagccacat taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa
    32161 ccacaagtga gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa
    32221 tttgttgatc tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta
    32281 aaagtgaata tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac
    32341 gataaattag ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc
    32401 agcaatttga ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat cagtgattta
    32461 ctcaatgata ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt
    32521 ttattagatc ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat
    32581 accgcaagga atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt
    32641 aatggaacat atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg
    32701 gctggtgaaa tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat
    32761 tatagaacaa gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat
    32821 attatggagc tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc
    32881 atgaaaactg gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg
    32941 tgggataatg gtaataacag accgtttcta tcttttggtg cgcccaattt caggtatagc
    33001 aatgctagtt atgtaacttc tggcgacggt agttctttaa gcattaatgg tagtgcgggt
    33061 aactcatggg aatttaaggt agctggtagg actatgaaat ttactagtga tggtatgcta
    33121 acgttaccag gttgtttttt tggttcatgg gaagatggga aacttgctag gtttgaacaa
    33181 tcaacggtac aagtatataa agattttact gttagaggta ctaaaaactc aactgtacca
    33241 acagaacatt atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct
    33301 gattatgggg aagccgttac aggtgacgat ggtaaagttc gtgttgatat tgaccccatg
    33361 tttgctgaaa cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt
    33421 ttgtgtgctg ttactcatga agatattgac catttcatca ttgaaactag taagccaaac
    33481 gtattagtta gatggaattt agtggcacac cgtctagggt atgaagatat tagattaaaa
    33541 gaggatacag catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag
    33601 gaggtatata aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa
    33661 actacaccaa agctattatg gctcgtcaag gtgataaagg gattacggtg acggttaaac
    33721 cgtttctaaa tggcttgcag atggatacga gtggcggaac atttacttta aaaggaacaa
    33781 caccatctaa ccgttacgta gataatgttg caactagtgt aactagtgaa gaagtcacgt
    33841 tttctcttga tggcacattt atgagtgaag caggatatta taaacactgc tacgtagaat
    33901 atagaaaaga caatcaaatt ttaacaacgc aagatatcat ttttttctca ctaggagtgt
    33961 ctgacatttc gcaaggccaa gccgatgaat atgtttcgca attagaagag ttgattcgaa
    34021 agtacaacga aacttttgat gcttttatgg ctgaaatcaa aggtagagtg gatagcttaa
    34081 atcaacagat tactgattta actggtcaag ctaaaacgct acaagacaag ttagatgctc
    34141 tgaaagaaga aatttctaag ttaggtaact tacaagtgat gtacagtaac agcatcgact
    34201 tcgggggata tgattatagc ggtaagccga atttaatgtc caaactaaaa tcgagcgatt
    34261 ttaacgttgg ttaccacggg tcactaactt tggataacga aaagttacat tttacttctg
    34321 atggtacagg aagcattatt atgtttacgc gtattaatac acctcagctt actagtggga
    34381 aaacctatac tctgagtgcg aaagttcgat ttgatgaagg aactacagga gctattgata
    34441 aattacgttt ggtgtatcgt acatcaccag gagaaaagat attattggaa gcaaatagta
    34501 caaatattac aacagatgat gtagggaaag aaataacaat caaaggtaca gctaacgtta
    34561 attatcaaat cacaaattta gatcgatttt atatgagtat tagctttgtt gacagggata
    34621 aaataaatgg cggatttaag ttgtacgaca tcaaaatcga agaaggctca acagccacac
    34681 cgtaccagcc taatctactt gatgcaccgt attatttgag taaggtggct ttgggtgaga
    34741 atctaattaa accagaatca caacaaccag ttactaatag taactatctt attaacacct
    34801 ataacattaa accaatggta aaaggtaaga agtataccat cacacttgaa ggaactaagc
    34861 cagcaacaca ggtttttaga ccatttttca cacgagcaac aggagatgca tgggaggttg
    34921 gtgacttaca accagtagaa ggcttaacta atgtgtggtc taaaacattt acagcagcag
    34981 atgactcaca ccctactacc ccacaagtgc agatttatca agtaccaagc acaagtgtag
    35041 gacaatgtac aattaagtgg ttaaaactag aggaaggcaa tactcgaacc ccgaatatta
    35101 gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa gattatgttt
    35161 gggatctagc accagaatat gtcgaagata atttggccac agatattaaa atttctgaaa
    35221 ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat tcgcagttga
    35281 ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcaaacaaaa gcgaatgcta
    35341 atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat gatacagcta
    35401 ctaccacatt aacagttaca aacgggaata ccggatcagc aaagctttat cgtgaaggaa
    35461 aaacagtttc tatatatttt gtggctttaa acggaaaacg cagtggtgga aatgattcaa
    35521 cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa ctggttggct
    35581 cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat ggagccatta
    35641 aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact tacacgattt
    35701 agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta cggattcgag
    35761 caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt cgaaccaccg
    35821 attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa aacatttgga
    35881 aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg cttgtatccg
    35941 ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa ccgttaacaa
    36001 tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg aacgcactag
    36061 atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat gagaacggcg
    36121 atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa caggaaaata
    36181 ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg atgcttgctt
    36241 cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca gcttcaaagg
    36301 gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg gggctgtttc
    36361 actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa agcagataaa
    36421 atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag
    36481 ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa ccgctatcgt
    36541 ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga ttactgagac
    36601 tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc
    36661 agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag atacacatgc
    36721 agaaaaattg gtagaacaca cgcaacaaat taaaacttt
//
LOCUS       DAFFFQ010000018        35882 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000018
VERSION     DAFFFQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.018"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1152
                     /locus_tag="LQB69_001027"
     CDS             <1..1152
                     /locus_tag="LQB69_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBL3525205.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="LQB69_001028"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="LQB69_001028"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL3525206.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="LQB69_001029"
     CDS             2728..3003
                     /locus_tag="LQB69_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL3525207.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="LQB69_001030"
     CDS             3009..3776
                     /locus_tag="LQB69_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL3525208.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="LQB69_001031"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="LQB69_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL3525209.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEECLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="LQB69_001032"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="LQB69_001032"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL3525210.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGRNKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="LQB69_001033"
     CDS             7920..8222
                     /locus_tag="LQB69_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL3525211.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="LQB69_001034"
     CDS             8225..9388
                     /locus_tag="LQB69_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3525212.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="LQB69_001035"
     CDS             9414..12842
                     /locus_tag="LQB69_001035"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL3525213.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="LQB69_001036"
     CDS             12863..13987
                     /locus_tag="LQB69_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525214.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="LQB69_001037"
     CDS             13977..14414
                     /locus_tag="LQB69_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3525215.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="LQB69_001038"
     CDS             14398..14736
                     /locus_tag="LQB69_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL3525216.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="LQB69_001039"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="LQB69_001039"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL3525217.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="LQB69_001040"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="LQB69_001040"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL3525218.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="LQB69_001041"
     CDS             15893..16936
                     /locus_tag="LQB69_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL3525219.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="LQB69_001042"
     CDS             17095..17823
                     /locus_tag="LQB69_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL3525220.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="LQB69_001043"
     CDS             17939..18184
                     /locus_tag="LQB69_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3525221.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="LQB69_001044"
     CDS             18181..18399
                     /locus_tag="LQB69_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL3525222.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="LQB69_001045"
     CDS             18535..19074
                     /locus_tag="LQB69_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL3525223.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="LQB69_001046"
     CDS             19067..21340
                     /locus_tag="LQB69_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL3525224.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="LQB69_001047"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="LQB69_001047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL3525225.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="LQB69_001048"
     CDS             complement(22513..22767)
                     /locus_tag="LQB69_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL3525226.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="LQB69_001049"
     CDS             complement(23167..23436)
                     /locus_tag="LQB69_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525227.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="LQB69_001050"
     CDS             complement(23906..24361)
                     /locus_tag="LQB69_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL3525228.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="LQB69_001051"
     CDS             complement(24499..26427)
                     /locus_tag="LQB69_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL3525229.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="LQB69_001052"
     CDS             complement(26624..27457)
                     /locus_tag="LQB69_001052"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL3525230.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="LQB69_001053"
     CDS             complement(27649..28353)
                     /locus_tag="LQB69_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3525231.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="LQB69_001054"
     CDS             complement(28376..29002)
                     /locus_tag="LQB69_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3525232.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="LQB69_001055"
     CDS             29328..31703
                     /locus_tag="LQB69_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL3525233.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="LQB69_001056"
     CDS             complement(31727..32218)
                     /locus_tag="LQB69_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL3525234.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="LQB69_001057"
     CDS             32405..33325
                     /locus_tag="LQB69_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3525235.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="LQB69_001058"
     CDS             33479..33823
                     /locus_tag="LQB69_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL3525236.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="LQB69_001059"
     CDS             complement(34013..34513)
                     /locus_tag="LQB69_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525237.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="LQB69_001060"
     tRNA            35041..35124
                     /locus_tag="LQB69_001060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="LQB69_001061"
     CDS             complement(35448..>35882)
                     /locus_tag="LQB69_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL3525238.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11869 a         6260 c         7405 g        10348 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaatgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtaga
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DAFFFQ010000019        35017 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000019
VERSION     DAFFFQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.019"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(54..2651)
                     /gene="adhE"
                     /locus_tag="LQB69_001062"
                     /gene_synonym="adhC"
     CDS             complement(54..2651)
                     /gene="adhE"
                     /locus_tag="LQB69_001062"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL3525239.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(2956..3336)
                     /gene="yajC"
                     /locus_tag="LQB69_001063"
     CDS             complement(2956..3336)
                     /gene="yajC"
                     /locus_tag="LQB69_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL3525240.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(3433..4578)
                     /gene="tgt"
                     /locus_tag="LQB69_001064"
     CDS             complement(3433..4578)
                     /gene="tgt"
                     /locus_tag="LQB69_001064"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL3525241.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            4796..5494
                     /locus_tag="LQB69_001065"
     CDS             4796..5494
                     /locus_tag="LQB69_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL3525242.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(5662..8301)
                     /locus_tag="LQB69_001066"
     CDS             complement(5662..8301)
                     /locus_tag="LQB69_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3525243.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(8920..9951)
                     /gene="queA"
                     /locus_tag="LQB69_001067"
     CDS             complement(8920..9951)
                     /gene="queA"
                     /locus_tag="LQB69_001067"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL3525244.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            10144..11319
                     /locus_tag="LQB69_001068"
     CDS             10144..11319
                     /locus_tag="LQB69_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3525245.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11323..11964
                     /locus_tag="LQB69_001069"
     CDS             11323..11964
                     /locus_tag="LQB69_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525246.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            11961..12878
                     /locus_tag="LQB69_001070"
     CDS             11961..12878
                     /locus_tag="LQB69_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3525247.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            12875..13546
                     /locus_tag="LQB69_001071"
     CDS             12875..13546
                     /locus_tag="LQB69_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525248.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(14087..14575)
                     /locus_tag="LQB69_001072"
     CDS             complement(14087..14575)
                     /locus_tag="LQB69_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL3525249.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(14776..15042)
                     /locus_tag="LQB69_001073"
     CDS             complement(14776..15042)
                     /locus_tag="LQB69_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL3525250.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(15042..15305)
                     /locus_tag="LQB69_001074"
     CDS             complement(15042..15305)
                     /locus_tag="LQB69_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3525251.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            15781..15966
                     /locus_tag="LQB69_001075"
     CDS             15781..15966
                     /locus_tag="LQB69_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3525252.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(16108..17931)
                     /locus_tag="LQB69_001076"
     CDS             complement(16108..17931)
                     /locus_tag="LQB69_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL3525253.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(18529..21234)
                     /locus_tag="LQB69_001077"
     CDS             complement(18529..21234)
                     /locus_tag="LQB69_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL3525254.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(21540..22487)
                     /locus_tag="LQB69_001078"
     CDS             complement(21540..22487)
                     /locus_tag="LQB69_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL3525255.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            22677..22946
                     /gene="rpsN"
                     /locus_tag="LQB69_001079"
     CDS             22677..22946
                     /gene="rpsN"
                     /locus_tag="LQB69_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL3525256.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(23122..23484)
                     /locus_tag="LQB69_001080"
     CDS             complement(23122..23484)
                     /locus_tag="LQB69_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3525257.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(23495..24616)
                     /locus_tag="LQB69_001081"
     CDS             complement(23495..24616)
                     /locus_tag="LQB69_001081"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL3525258.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(24632..24982)
                     /locus_tag="LQB69_001082"
     CDS             complement(24632..24982)
                     /locus_tag="LQB69_001082"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL3525259.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(25112..26623)
                     /locus_tag="LQB69_001083"
     CDS             complement(25112..26623)
                     /locus_tag="LQB69_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL3525260.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(26945..28297)
                     /locus_tag="LQB69_001084"
     CDS             complement(26945..28297)
                     /locus_tag="LQB69_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL3525261.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(28450..29526)
                     /locus_tag="LQB69_001085"
     CDS             complement(28450..29526)
                     /locus_tag="LQB69_001085"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL3525262.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            29722..31467
                     /locus_tag="LQB69_001086"
     CDS             29722..31467
                     /locus_tag="LQB69_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL3525263.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(31747..32184)
                     /locus_tag="LQB69_001087"
     CDS             complement(31747..32184)
                     /locus_tag="LQB69_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL3525264.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(32168..33541)
                     /locus_tag="LQB69_001088"
     CDS             complement(32168..33541)
                     /locus_tag="LQB69_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525265.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(33863..34351)
                     /locus_tag="LQB69_001089"
     CDS             complement(33863..34351)
                     /locus_tag="LQB69_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525266.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(34373..34975)
                     /locus_tag="LQB69_001090"
     CDS             complement(34373..34975)
                     /locus_tag="LQB69_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3525267.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9919 a         7476 c         6466 g        11156 t
ORIGIN      
        1 ttccaagttc tttttcatct attttcttat cttttttcta cgctttcttt tgcttatccg
       61 ttgtaagcat caatgatgat ctgtttcaat tcagagatca atggttcttt cggattagct
      121 gtcgtacatt ggtcttcgta agctaattca gccatacggt ctacggttgt atctagtgtt
      181 tcttgtgtga ctccttgagc ttttagactc atgttgatgc cgacagattt tcctaattca
      241 taaacagccg ttgctaaagc ttcgactaat tcctctgttg tttcgccttt cagacctaag
      301 aattttgcga tatctgcgta atctgtatct gcacggaaat agtcatattt agggaacatc
      361 gcatgttttt gtggatcttt tgcattatag cggataatat gcggtaaaag aatcgcattt
      421 gtccgtccat gagggatccc gtattccccg ccgattttat gtgcgactga gtgacaaata
      481 cctaggaatg cgttagcaaa cgccatacca gccattgtag aagcgttgtg cattttttca
      541 cgtgattcca tatcaggtgt ttttactgat ttttctaaat agtcaaagac taacttgatt
      601 gcttgtaggc tcaatccacg tgtataatct gaagccatga ctgaaacata ggattcgatt
      661 gcatgtgtta acacatccat cccggtatct gctgtgacag atgctggaac tgacatgacg
      721 aattgaggat cgacgattgc gacatctggc gtcaatgcat aatctgctaa cggatatttc
      781 acatgtgttt cactgtcagt gatgaccgca aatggcgtta cttcagaacc agtccctgat
      841 gttgtcggga tacatacgaa ttgtgttttt tctggtttgt cgatcttata tgtccgttta
      901 cggatatcca agaatttttg ttttgcacca aagaaagatg tgtctgggtg ttcatagaac
      961 atccacattc ccttagctgc gtccattgca gaaccgcctc caagagcgat tactgtatct
     1021 ggcttgaagt ctgccatcag tttcgttcct gcgtatacgg tatttgtcga tgggttcggt
     1081 tcaacatctg agaagacttc gatcttcaca tcatttttac gtcgtgccaa tacttcacga
     1141 actgtatcgc agtaaccgaa ttggaccata ccagggtcac aaacgatcat gacacgttca
     1201 acgttttcca ttttctctaa gtacaataga gagttctttt caaagaagat ttttggcggt
     1261 aatttgaacc attgcatgtt atttctccgt ttcgctacag ttttgacatt gattaagttt
     1321 acagcagaga cgttacgaga aacagagttc ttaccataag aaccgcatcc caaagtcaat
     1381 gacggaatca tttcgttata gatatcaccg atgccgcctt ctgcagatgg tgtattcact
     1441 aaaatacgac aagctttcat tcgaagaccg aatttgacat gcaactcttc gttttctgaa
     1501 tggataactg ctgtatgtcc taatcctcca agattcaaca taccttcaca caaatcaaac
     1561 gcatggtccg tgttgttagc tttgatcatg gcaagaactg gagaaagttt ttctcttgat
     1621 agcggataat ctgggcctac gccttccaat tcagcaatca gcattttcgt tccttttggt
     1681 acactgattc ctgccaattt tgcgatttcc atcgcagaat gtccaacgat tgatggattg
     1741 accgcatatt ttccttcgtt catcactgca tcttctaatt tttgcaattc atctggtttt
     1801 acgatataaa cttggtgtgc ttggaattct gcttttactg ctgcatagat ttctttatcc
     1861 acgatcacag cttgttcaga agcacaaatc attccattat caaatgtttt cgaaacgatc
     1921 aaatcgttca ctgcacgttt aatttttgct gttttttcaa tataagctgg tacgttacct
     1981 ggacctacac ctaaagctgg ttttcctgta gagtaagcag acttgaccat tccagctcca
     2041 cctgtcgcta agacgatcgc gatacctgga tggttcatta gcatagaagt cgcttcgatc
     2101 gaaggatgtt cgatccattg gatacaattt tcaggagctc cggcagcgat agcagcatca
     2161 cgtaccactc gtgctgcttc tgcagaagat ttttgggcac ttggatggaa agcaaaaacg
     2221 attggatttc ttgttttcaa cgcgatcatt gatttgaaaa tcgttgtgga agtcggattc
     2281 gttgttggag ttactccgca aaccacaccg actggttcag caatttcaat caaccctttt
     2341 tggacatctt cattgatcac accaactgtt ttatcatgtt tgatattatg ccaaatatat
     2401 tcggacgcat acatattttt gattgcttta tcttcgtaaa caccgcggcc tgtttcctct
     2461 acagccatct tcgctaatgg catatgttga tccaaagctg ccattgccat ttcatgcacg
     2521 atatggtcta ctttttcttg atcaaagctt tccatttctt tcaatgctgc atttgctttt
     2581 tctgccaaat catcaatcat tgcttgtacg tcaattgtct tcgttttttc tttttctatt
     2641 actttagcca tcattatcct cctaatgggt catggttatt tgttaaataa ttcacatgta
     2701 tatcatacaa tatttatttt tctatgtaaa cacttttttg tgattttttt cacagttatt
     2761 tttaaatata aaattgcttt atttacaggt gtttgtatag aaaaatatat aaagttattt
     2821 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
     2881 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
     2941 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
     3001 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
     3061 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
     3121 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
     3181 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
     3241 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
     3301 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
     3361 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
     3421 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
     3481 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
     3541 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
     3601 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
     3661 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
     3721 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
     3781 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
     3841 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
     3901 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
     3961 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
     4021 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
     4081 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
     4141 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
     4201 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
     4261 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
     4321 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
     4381 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
     4441 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
     4501 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
     4561 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
     4621 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
     4681 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
     4741 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
     4801 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
     4861 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
     4921 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
     4981 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
     5041 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
     5101 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
     5161 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
     5221 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
     5281 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
     5341 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
     5401 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
     5461 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
     5521 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
     5581 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
     5641 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
     5701 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
     5761 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
     5821 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
     5881 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
     5941 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
     6001 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
     6061 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
     6121 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
     6181 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
     6241 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
     6301 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
     6361 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
     6421 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
     6481 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
     6541 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
     6601 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
     6661 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
     6721 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
     6781 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
     6841 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
     6901 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
     6961 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
     7021 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
     7081 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
     7141 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
     7201 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
     7261 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
     7321 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
     7381 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
     7441 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
     7501 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
     7561 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
     7621 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
     7681 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
     7741 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
     7801 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
     7861 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
     7921 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
     7981 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
     8041 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
     8101 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
     8161 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
     8221 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
     8281 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
     8341 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
     8401 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
     8461 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
     8521 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
     8581 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
     8641 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
     8701 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
     8761 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
     8821 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
     8881 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
     8941 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
     9001 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
     9061 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
     9121 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
     9181 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
     9241 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
     9301 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
     9361 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
     9421 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
     9481 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
     9541 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
     9601 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
     9661 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
     9721 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
     9781 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
     9841 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
     9901 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
     9961 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
    10021 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
    10081 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
    10141 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
    10201 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
    10261 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
    10321 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
    10381 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
    10441 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
    10501 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
    10561 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
    10621 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
    10681 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
    10741 attaaactag caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca
    10801 ccagaaaata ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat
    10861 cgcctactac aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca
    10921 atcacgatta caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt
    10981 gtagatacgt tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa
    11041 acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt
    11101 ttttacgtaa aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg
    11161 ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca
    11221 tcattagtgg atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt
    11281 gcagagtcag ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat
    11341 cagcacggtt cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct
    11401 ttgatcattg gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa
    11461 gctgccacga ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta
    11521 ttggcattga tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt
    11581 atttattcat tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg
    11641 aattatcggg atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt
    11701 gaattgccca ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg
    11761 attgcatggg ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt
    11821 agtggactga ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt
    11881 ttagcactat tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca
    11941 cttagagagg agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc
    12001 ttcttatact tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca
    12061 tttcgatcac aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa
    12121 tggttgaaca ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta
    12181 tcaaccatca agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa
    12241 cagatttaac gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta
    12301 ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatggttttg
    12361 aaaatacgta tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag
    12421 tcagcgatct gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca
    12481 atcgtaaagg agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga
    12541 tattacctat gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg
    12601 ttcttggcta ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg
    12661 atcgtcaatt cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg
    12721 atacgcctca tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa
    12781 tgcaggaatt gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac
    12841 gtttcttaca agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt
    12901 ctttcaacaa tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat
    12961 ccgtcacttc ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt
    13021 tgggatcatg atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat
    13081 catccagacg atcccttctc tagccatgat ctccatttta atcattggat taggcctggg
    13141 agtcaatgtc gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac
    13201 gtataccggg atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat
    13261 gactgctaga caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc
    13321 aggcataaga aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg
    13381 tgctggtggt cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat
    13441 tattttagca ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg
    13501 tattttggaa agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa
    13561 acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa
    13621 ggcaataatc cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat
    13681 ttatccattc cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga
    13741 ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat
    13801 ggaattcata ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa
    13861 ttcttacatc ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg
    13921 atgccttcag ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt
    13981 tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc
    14041 ttctcgccat gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc
    14101 cgtcgcaact agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca
    14161 attgatcgat atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg
    14221 atgcattgcc gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg
    14281 tttgagtgac attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga
    14341 cactggtatt gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg
    14401 ggaaccaata agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg
    14461 tttctcgata aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg
    14521 ttgccactgc caacactaac aacgatgctc caattttact gattttcctt gtcacacttc
    14581 ttcctccttg ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct
    14641 tcaaaggtgg agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg
    14701 ttttagaaca aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg
    14761 ttggctcttc tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt
    14821 ttcctcatca actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc
    14881 ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa
    14941 tgtctgaatc acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc
    15001 cactttcgca gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca
    15061 atatcatcga tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa
    15121 gtacctgttt gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc
    15181 aatacagcat tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt
    15241 acctctttca ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta
    15301 gccattgtct ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa
    15361 aaaatacaat aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag
    15421 accccttttt agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt
    15481 agccaaacgt aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc
    15541 ctgtgagtct atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt
    15601 gatatcttga ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat
    15661 tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc
    15721 atgttcacta tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa
    15781 atggcactga gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat
    15841 gaagacacat ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt
    15901 acattatttg gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac
    15961 agctaaatag gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga
    16021 gggcaccttt agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag
    16081 ggtctgtttt tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac
    16141 aacgatattt tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag
    16201 aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt
    16261 ttgtttcgct gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga
    16321 gcgataagaa gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc
    16381 ttcgatttct ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata
    16441 atcaccgatc gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac
    16501 taatacaaga acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag
    16561 ctgttctgct acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg
    16621 cataatgatg aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat
    16681 tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc
    16741 ttcttttttc catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc
    16801 gatagagtat aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat
    16861 cgaacctagc gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg
    16921 atacatgtga ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga
    16981 aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata
    17041 caaaatacca tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc
    17101 aggaacgatc ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat
    17161 caatgccatc atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg
    17221 tacagcatta ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg
    17281 taaaatcaaa gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa
    17341 tggcacagca cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag
    17401 gataaatgtg tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt
    17461 cagcatcatc agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc
    17521 tggcaccgct gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat
    17581 gtagtcgatc aataaagagc caccggaaat caagccagca ttctttccaa gattctcact
    17641 gctgacgaca tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag
    17701 cgttaatgaa atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc
    17761 tgcagcagac aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga
    17821 taacgcatca gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt
    17881 ttcagcagat tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac
    17941 tcctttacac acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt
    18001 tatagtacgc tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac
    18061 agaaaaatat caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta
    18121 tgggcttttt ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc
    18181 tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat
    18241 tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc
    18301 ttgcatctgc ggtaataagc tcaaccaaat taaaaacata agaagctgtt tttatttggt
    18361 cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat
    18421 cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt
    18481 tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat
    18541 atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat
    18601 tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt
    18661 agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa
    18721 tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc
    18781 gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc
    18841 gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa
    18901 tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt
    18961 aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg
    19021 ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac
    19081 acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc
    19141 tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa
    19201 gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac
    19261 ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg
    19321 ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt
    19381 ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg
    19441 gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag
    19501 gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt
    19561 ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc
    19621 cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc
    19681 cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga
    19741 agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt
    19801 aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac
    19861 agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt
    19921 tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag
    19981 gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt
    20041 caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt
    20101 tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt
    20161 aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt
    20221 tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc
    20281 ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt
    20341 ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat
    20401 ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat
    20461 tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac
    20521 gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag
    20581 gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc
    20641 tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac
    20701 tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga
    20761 acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata
    20821 ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc
    20881 ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc
    20941 ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg
    21001 ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc
    21061 aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc
    21121 atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa
    21181 aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct
    21241 cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg
    21301 gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc
    21361 ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa
    21421 agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt
    21481 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
    21541 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
    21601 gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct
    21661 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
    21721 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
    21781 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
    21841 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
    21901 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
    21961 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
    22021 ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
    22081 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
    22141 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
    22201 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
    22261 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
    22321 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
    22381 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
    22441 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
    22501 ctttttcttt aagtttacgt ttatgtcttt attttgacaa aaaaaatgtc ttttcgtttt
    22561 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
    22621 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
    22681 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
    22741 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag
    22801 atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca
    22861 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
    22921 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
    22981 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
    23041 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
    23101 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
    23161 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
    23221 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
    23281 tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt
    23341 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
    23401 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
    23461 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
    23521 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
    23581 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
    23641 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
    23701 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
    23761 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
    23821 ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg
    23881 gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta
    23941 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
    24001 tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt
    24061 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
    24121 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
    24181 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
    24241 tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg
    24301 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
    24361 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
    24421 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
    24481 gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
    24541 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
    24601 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
    24661 ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca
    24721 caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
    24781 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
    24841 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
    24901 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
    24961 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
    25021 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
    25081 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
    25141 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
    25201 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
    25261 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
    25321 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
    25381 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
    25441 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
    25501 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
    25561 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
    25621 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
    25681 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
    25741 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
    25801 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
    25861 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
    25921 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
    25981 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
    26041 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
    26101 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
    26161 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
    26221 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
    26281 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
    26341 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
    26401 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
    26461 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
    26521 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
    26581 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
    26641 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
    26701 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
    26761 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
    26821 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
    26881 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
    26941 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
    27001 cttttagcat aatactatct gttgctgcaa gattttgttt gatagcatca atcaatttat
    27061 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
    27121 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
    27181 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
    27241 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
    27301 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
    27361 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
    27421 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
    27481 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
    27541 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
    27601 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
    27661 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
    27721 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
    27781 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
    27841 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
    27901 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
    27961 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
    28021 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
    28081 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
    28141 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
    28201 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
    28261 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
    28321 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
    28381 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
    28441 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
    28501 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
    28561 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
    28621 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
    28681 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
    28741 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
    28801 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
    28861 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
    28921 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
    28981 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
    29041 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
    29101 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
    29161 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
    29221 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
    29281 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
    29341 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
    29401 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
    29461 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
    29521 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
    29581 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
    29641 gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
    29701 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
    29761 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
    29821 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
    29881 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
    29941 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
    30001 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
    30061 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
    30121 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
    30181 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
    30241 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
    30301 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
    30361 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
    30421 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
    30481 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
    30541 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
    30601 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
    30661 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
    30721 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
    30781 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
    30841 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
    30901 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
    30961 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
    31021 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
    31081 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
    31141 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
    31201 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
    31261 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
    31321 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
    31381 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
    31441 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    31501 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    31561 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    31621 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
    31681 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
    31741 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
    31801 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
    31861 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
    31921 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
    31981 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
    32041 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
    32101 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
    32161 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
    32221 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
    32281 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
    32341 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
    32401 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
    32461 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
    32521 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
    32581 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
    32641 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
    32701 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
    32761 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
    32821 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
    32881 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
    32941 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
    33001 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
    33061 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
    33121 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
    33181 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
    33241 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
    33301 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
    33361 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
    33421 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
    33481 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
    33541 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
    33601 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
    33661 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    33721 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    33781 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    33841 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    33901 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    33961 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    34021 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    34081 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    34141 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    34201 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    34261 caatgatcgg cacaaaagct tccggccata cttcttgtat gactgccatc agctcatcca
    34321 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    34381 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccatg
    34441 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    34501 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    34561 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    34621 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    34681 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    34741 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    34801 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    34861 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    34921 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    34981 ttccccacat cttttttcat ttattaaaac tgcctcc
//
LOCUS       DAFFFQ010000020        34636 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000020
VERSION     DAFFFQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.020"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            591..1280
                     /locus_tag="LQB69_001091"
     CDS             591..1280
                     /locus_tag="LQB69_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3525268.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="LQB69_001092"
     CDS             1267..2475
                     /locus_tag="LQB69_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3525269.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="LQB69_001093"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="LQB69_001093"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL3525270.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LQB69_001094"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="LQB69_001094"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL3525271.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="LQB69_001095"
     CDS             complement(6578..7273)
                     /locus_tag="LQB69_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL3525272.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LQB69_001096"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="LQB69_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL3525273.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="LQB69_001097"
     CDS             complement(8472..8663)
                     /locus_tag="LQB69_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL3525274.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="LQB69_001098"
     CDS             complement(8684..9574)
                     /locus_tag="LQB69_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL3525275.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="LQB69_001099"
     CDS             complement(9561..10328)
                     /locus_tag="LQB69_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3525276.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LQB69_001100"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="LQB69_001100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL3525277.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LQB69_001101"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="LQB69_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL3525278.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LQB69_001102"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="LQB69_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL3525279.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="LQB69_001103"
     CDS             14899..15282
                     /locus_tag="LQB69_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525280.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="LQB69_001104"
     CDS             complement(15450..16208)
                     /locus_tag="LQB69_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL3525281.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="LQB69_001105"
     CDS             complement(16226..17044)
                     /locus_tag="LQB69_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL3525282.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LQB69_001106"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="LQB69_001106"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL3525283.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LQB69_001107"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="LQB69_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL3525284.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="LQB69_001108"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="LQB69_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL3525285.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="LQB69_001109"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="LQB69_001109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL3525286.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="LQB69_001110"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="LQB69_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL3525287.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="LQB69_001111"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="LQB69_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL3525288.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="LQB69_001112"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="LQB69_001112"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL3525289.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="LQB69_001113"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="LQB69_001113"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL3525290.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="LQB69_001114"
     CDS             27086..27385
                     /locus_tag="LQB69_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL3525291.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="LQB69_001115"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="LQB69_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3525292.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="LQB69_001116"
     CDS             27973..28209
                     /locus_tag="LQB69_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL3525293.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="LQB69_001117"
     CDS             28352..30325
                     /locus_tag="LQB69_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL3525294.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEALLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="LQB69_001118"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="LQB69_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL3525295.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="LQB69_001119"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="LQB69_001119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL3525296.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="LQB69_001120"
     CDS             33204..34496
                     /locus_tag="LQB69_001120"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL3525297.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10553 a         6734 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggccctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAFFFQ010000021        33516 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000021
VERSION     DAFFFQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.021"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            216..953
                     /locus_tag="LQB69_001121"
     CDS             216..953
                     /locus_tag="LQB69_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525298.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(970..1386)
                     /locus_tag="LQB69_001122"
     CDS             complement(970..1386)
                     /locus_tag="LQB69_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525299.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            1778..3025
                     /locus_tag="LQB69_001123"
     CDS             1778..3025
                     /locus_tag="LQB69_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL3525300.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            3162..3305
                     /locus_tag="LQB69_001124"
     CDS             3162..3305
                     /locus_tag="LQB69_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525301.1"
                     /translation="MLSTKKVINRCGDFNLGMGAKVITTPTYRGSFSSPNWYKEGVTR
                     DEA"
     gene            3295..5685
                     /locus_tag="LQB69_001125"
     CDS             3295..5685
                     /locus_tag="LQB69_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBL3525302.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            5734..7569
                     /locus_tag="LQB69_001126"
     CDS             5734..7569
                     /locus_tag="LQB69_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3525303.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            7638..8501
                     /locus_tag="LQB69_001127"
     CDS             7638..8501
                     /locus_tag="LQB69_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3525304.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            8491..9600
                     /locus_tag="LQB69_001128"
     CDS             8491..9600
                     /locus_tag="LQB69_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3525305.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            9939..10739
                     /locus_tag="LQB69_001129"
     CDS             9939..10739
                     /locus_tag="LQB69_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525306.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            10827..11633
                     /locus_tag="LQB69_001130"
     CDS             10827..11633
                     /locus_tag="LQB69_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525307.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            11865..12734
                     /locus_tag="LQB69_001131"
     CDS             11865..12734
                     /locus_tag="LQB69_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL3525308.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            12754..13101
                     /locus_tag="LQB69_001132"
     CDS             12754..13101
                     /locus_tag="LQB69_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525309.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            13114..14376
                     /locus_tag="LQB69_001133"
     CDS             13114..14376
                     /locus_tag="LQB69_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525310.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            14456..15832
                     /locus_tag="LQB69_001134"
     CDS             14456..15832
                     /locus_tag="LQB69_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL3525311.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            15829..16458
                     /locus_tag="LQB69_001135"
     CDS             15829..16458
                     /locus_tag="LQB69_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525312.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            16477..16956
                     /locus_tag="LQB69_001136"
     CDS             16477..16956
                     /locus_tag="LQB69_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525313.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            17458..17511
                     /locus_tag="LQB69_001137"
                     /pseudo
     CDS             17458..17511
                     /locus_tag="LQB69_001137"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17515..18114
                     /locus_tag="LQB69_001138"
     CDS             17515..18114
                     /locus_tag="LQB69_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL3525314.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            18130..18606
                     /locus_tag="LQB69_001139"
     CDS             18130..18606
                     /locus_tag="LQB69_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525315.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            18623..19525
                     /locus_tag="LQB69_001140"
     CDS             18623..19525
                     /locus_tag="LQB69_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525316.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            19598..19792
                     /locus_tag="LQB69_001141"
     CDS             19598..19792
                     /locus_tag="LQB69_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525317.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            19897..20325
                     /locus_tag="LQB69_001142"
     CDS             19897..20325
                     /locus_tag="LQB69_001142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525318.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            20560..21147
                     /locus_tag="LQB69_001143"
     CDS             20560..21147
                     /locus_tag="LQB69_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525319.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            21147..22046
                     /locus_tag="LQB69_001144"
     CDS             21147..22046
                     /locus_tag="LQB69_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBL3525320.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            22046..22960
                     /locus_tag="LQB69_001145"
     CDS             22046..22960
                     /locus_tag="LQB69_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525321.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            23012..23227
                     /locus_tag="LQB69_001146"
     CDS             23012..23227
                     /locus_tag="LQB69_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525322.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            23417..23671
                     /locus_tag="LQB69_001147"
     CDS             23417..23671
                     /locus_tag="LQB69_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525323.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            23850..24899
                     /locus_tag="LQB69_001148"
     CDS             23850..24899
                     /locus_tag="LQB69_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL3525324.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            25076..25306
                     /locus_tag="LQB69_001149"
     CDS             25076..25306
                     /locus_tag="LQB69_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525325.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            25309..25566
                     /locus_tag="LQB69_001150"
     CDS             25309..25566
                     /locus_tag="LQB69_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525326.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            25783..26658
                     /locus_tag="LQB69_001151"
     CDS             25783..26658
                     /locus_tag="LQB69_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525327.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            26668..27462
                     /locus_tag="LQB69_001152"
     CDS             26668..27462
                     /locus_tag="LQB69_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525328.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            27491..28609
                     /locus_tag="LQB69_001153"
     CDS             27491..28609
                     /locus_tag="LQB69_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525329.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            28642..28962
                     /locus_tag="LQB69_001154"
     CDS             28642..28962
                     /locus_tag="LQB69_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525330.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            29217..29906
                     /locus_tag="LQB69_001155"
     CDS             29217..29906
                     /locus_tag="LQB69_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HBL3525331.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            29943..30110
                     /locus_tag="LQB69_001156"
     CDS             29943..30110
                     /locus_tag="LQB69_001156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525332.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            30130..31758
                     /locus_tag="LQB69_001157"
     CDS             30130..31758
                     /locus_tag="LQB69_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525333.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            31809..32180
                     /locus_tag="LQB69_001158"
     CDS             31809..32180
                     /locus_tag="LQB69_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525334.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            32265..32465
                     /locus_tag="LQB69_001159"
     CDS             32265..32465
                     /locus_tag="LQB69_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525335.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            32599..>33516
                     /locus_tag="LQB69_001160"
     CDS             32599..>33516
                     /locus_tag="LQB69_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525336.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRN"
BASE COUNT        12784 a         4502 c         6745 g         9485 t
ORIGIN      
        1 aaagtcaaaa gtaaatactt tcttaaaaag tcaaataaaa ctattaaaga caatatatat
       61 ggtttatttg acataagaaa atggtctaga tgataattgt aatatagaat tgtaggctta
      121 aataaagcat aaaatacctt ttatgataag gtatagctct taattttaag cagtttttag
      181 atataagagt gaaagtaaag tgggggagaa taataatggc agatcgtaaa ttagaaaagt
      241 tattagaaga aacgtggaat ccaaaagaat ttagtgaatt ttttatggag aattttgaaa
      301 ctgacttagc agtgattgtt aaagatgctc ttagagagca agggtatcct gaaactgcaa
      361 actatataaa cattaatttt actttgtata ctgaaaataa aggaacttgg gatttttggg
      421 ctactttagc aaataaggag ttaagtgata aatcagacac aggaattcgt aacttttttg
      481 aatcaaacag agatgattat atgtatgcta accatcaaga taaattgaac tttagagtag
      541 agtttgatga aacacctgaa gaatttatag aaagacaacc gcctaaagaa aatgtagcaa
      601 aggtattgga agatcgttgg aattcagatg aaatagtttc aacaattagc gaactaggcg
      661 gacaatatga accgctagta gaagcggtta gagaagagct aagactgaat aagtttcctg
      721 atgttcaaaa tattgatgtt tctcaaattg aaataaacgt caagatcacg aataaacttg
      781 attatggtag ttgggcagat atagcattag aaaaatacat ctactcaacg ttaaaagagt
      841 ttatcgaaaa tagaatggat atcatgtatc tacagcatcc acagtattta aactttggtg
      901 ttgaaatagc tacacctctt gaagaatgga aaatggagca agggcttgac tagcctttgc
      961 tccatttttt tattttacaa atgaatacag ttcgtcgttt ttaccaagaa tgattgtttg
     1021 cccaactttg agattgtcag gtgatccttc agcggtttgt aaggagaacg ttccgttgaa
     1081 cttatcggat accgttatag gtagctttaa ttctgatcct tcctcatctt tgagaattgc
     1141 tgtttttcgt tcatcaaaat aaacagtctt tattaaataa tttttcgtag tgatctcagc
     1201 agatttgtct ttttttgtag tagtaacagt ttctttagaa ttagaggtat tttctacgac
     1261 tattccatct ttggcatttg agcttgtgtt tatttgattg aatattttca gcgacaagat
     1321 accgctaaga atagtcaata tagaaacaag tgtaattaat actgataatt tccttttttt
     1381 gttcattttt tcttaacttc ctttttgttt tgattagtta gatattcctt atctaacaag
     1441 cctttttagg ctattaaatg aagataaacc ccaaaaaaat attgcaatta tgacagtgca
     1501 atggtactat tattatacaa taaattttgc gaaaggggta ggtctaaatc gcaaacaaaa
     1561 agaaattgaa cctagacaaa tacaacttgt acaccgcaag ggaagcaagt ttgattttgg
     1621 gtaaaggtag aaattatgtt acaaacataa tcaactatta tccaaatcgt ttgcctaaag
     1681 gttcgttcaa gaaagtgggt gacaaaacct atattataac ggaagaagga atagaagcat
     1741 tgcgatcaca aacagaaaat aaaaacaaaa agaaggaatg aatatgaaga aaaattcgct
     1801 tggtatggtt ttatttgcat cagctgtact tgcggggtta tcagtaccag taacctcaca
     1861 tgctgatgaa tggactgcaa acacaccgga tcaaatccaa attacaacag gacaatcaag
     1921 ttatgcgttc aaaaaaggag atactatttg gtcaactgta gagaagctta aaagcttagg
     1981 ttacaagact actgttgctg agtttgccga attgaacaat gttgatttgg caaaaggaga
     2041 acaacggact atcccaattg gacgaacttt caacatcagt aaagatacat taacagttaa
     2101 ggaagcagat ggcgaagtag ttagtcaaac tgttcaaaaa gataaacctg atcctgttga
     2161 aaatacggag caagagaaca aaaaatccga gactgcaaaa gaatcggaac caacagcaaa
     2221 cacttctgat aagacagaaa caagtgcttc aagtgttgat ggagcaagtt catctaatac
     2281 aaataattct acaagtgaga aagaagcatc acagggaaca agtgaaagta gcactacgga
     2341 aactgatact ggaacatcaa caaataactc acaatcaaca tcaaatgatg caaatgaatc
     2401 aaccagtaca tcttctagtg atcaatcaca aacaggttcg agtgatgaaa ctgacagttc
     2461 tacttcacca agtaaacccg aacaaccaac taatgttgtg ctaggcgttc catttattag
     2521 tcaaggtaac acaatgcttt gcgaaggaac atctttgcta gaagcgttac attataaagg
     2581 tgttgcaaca aatcaagatt taatgacttt tgttaaatca atgccactat ctcctgataa
     2641 taatccaaat aatggttttt ctggtgaatg gagacacaat gtggatggaa cttatcaagg
     2701 tatgaatccg gctcctgtag tttcatgggg tgttgctaat ggcggaaatg ttgtagatat
     2761 ttctggttca tcggtacaag gattaaaaga tgagattgta aatggaaatc ctgtagtagc
     2821 gtgggtaact tatgcctttg aacctgctca acataaatca atgttttggg gcgatgctat
     2881 ttggaatcga catgtagtag ccgttgatgg atttaaagac ggtttttacc atgttgtcga
     2941 tcctgtgttt ggggcaagtt ggattaatgc aggatcattt gaaacagcat ataacgtatc
     3001 tcaattagcg gtttcggtac gttaataaaa gagattgttt atatactaag tagttaagta
     3061 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
     3121 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaag
     3181 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
     3241 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
     3301 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
     3361 gtgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
     3421 ccgacaagtg caaaatttac agaaactggc gtaaaaacag acggttatat tagggttaat
     3481 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
     3541 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
     3601 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
     3661 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
     3721 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
     3781 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
     3841 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
     3901 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
     3961 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
     4021 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
     4081 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
     4141 tttgatggac gaggcgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
     4201 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
     4261 gttgatcaag atggaaatgc tttaaaagga gcggagttca ctctaactga caataacaat
     4321 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
     4381 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
     4441 gatggtatta ctgttaatac cgatggacaa aaatttagtt acactgctac gaacacaatt
     4501 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
     4561 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
     4621 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
     4681 actggttata caggcgcatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
     4741 tttgaatata cagctacaaa cactttgaaa aaaggtgtag taaaagttca taaagtcgat
     4801 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
     4861 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
     4921 tatagtctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
     4981 atcagtgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
     5041 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
     5101 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
     5161 acgttcaaca ttattgcgaa caaaacttac aacttagaag aaacaaaagt accaacaggt
     5221 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
     5281 tatacagcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt cgatgatcaa
     5341 gggaaagcat tagcaaacgc agaattcact ttgacggacg acaaaggtgt aaaagttgta
     5401 caaaaaacgg acagaaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
     5461 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
     5521 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
     5581 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
     5641 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
     5701 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
     5761 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
     5821 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
     5881 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
     5941 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
     6001 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
     6061 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
     6121 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
     6181 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
     6241 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
     6301 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
     6361 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
     6421 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
     6481 actgtggatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
     6541 gctaatagaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
     6601 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
     6661 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
     6721 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
     6781 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
     6841 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
     6901 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
     6961 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
     7021 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
     7081 aaaacagacg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
     7141 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg
     7201 attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt
     7261 gaaggtttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
     7321 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
     7381 actagaaacg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
     7441 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
     7501 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
     7561 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
     7621 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
     7681 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
     7741 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
     7801 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
     7861 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
     7921 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
     7981 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
     8041 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
     8101 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
     8161 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
     8221 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
     8281 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
     8341 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
     8401 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
     8461 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
     8521 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
     8581 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
     8641 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
     8701 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
     8761 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
     8821 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
     8881 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
     8941 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
     9001 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
     9061 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
     9121 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
     9181 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
     9241 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
     9301 gctgatgcca ttgatagtga atccgtttca gataagccag aaactgttac atcttctaat
     9361 gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
     9421 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
     9481 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtatta
     9541 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttag aaaagcataa
     9601 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
     9661 tttttatttt gaatagagct agattcactt ttaaataaaa taaaaactga tcttaattcc
     9721 aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat
     9781 tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat
     9841 aatgtatgga aaagctataa agcaatagat aagatgtaga aaatcttatt tgtgaatgaa
     9901 aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtggaaata tcaaaaacag
     9961 ctttagactc attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat
    10021 atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac actgaaaaaa gactatttga
    10081 atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat gtagtgtgta
    10141 cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata
    10201 cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa
    10261 tgggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa
    10321 ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaga tgacagagaa aaagtaaaat
    10381 tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag
    10441 acagcaacta ttttcctgaa ttgaaggtat atcaaaacga cgaagatttc tttaatgaaa
    10501 tgtttagtaa tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa tggaactttc
    10561 aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt
    10621 atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag
    10681 agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg
    10741 gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagc
    10801 cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg
    10861 atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat gtagaagact
    10921 ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg
    10981 aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatc gcatcgaaac
    11041 taagtcaatt tgccgaggag aaatcgtgga atgaattagt tagtttttcc gaaaaaattt
    11101 atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa
    11161 ttgatgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata
    11221 ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa
    11281 aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat acagaggtga
    11341 aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag
    11401 aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtata
    11461 tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa gaaactgttc
    11521 gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa
    11581 actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag
    11641 ctgaaaagct ctattttttt tgataaaaaa actagctatt tttttaaaaa taatttggtt
    11701 caaaatttat ttttttaact tgataatttt ttgccatcct tttttaaatt ggctatatat
    11761 caaggtttat agtggggata aataaaaata catttgacaa aatttacaat atagtttatt
    11821 atatatttat cttattatga taacacaata aaaggaatga tggtatgaaa aagaagaagt
    11881 ttagtcgatc gttgttagga attttagttt tagttgcggt tggtcttagt ggcaagggtt
    11941 actttcagaa taacagcact caggaacatg attttcagat caaaagtagt gttcaggtag
    12001 gtaataaagc agacagtgaa tcaaaagaat ttttagggat acgttatgat ccaaacaaat
    12061 atccaaataa tatcgtaaac attaatgatg ggaaatatac actgacaaaa gaagaccatg
    12121 aagcattatc agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat ttagatcaac
    12181 ttggaagagc aggagatgtt gaagcgttga tcacctatga tgctataaat tctcactcat
    12241 ctaaaaatgt ggaacgtcca tctttcaaaa gttctgtcca tattgcagga gaatacaagg
    12301 atggagtatt tgatcaagag agagatactt gggtcggcaa agattcaaac aatgaaatca
    12361 ctcagctaga aggatataaa ggttatctct ataacaaaag tcattcatta gcatggtcgc
    12421 taggaggaga tatggaggta cacaatttaa cacttggaac gagagcgcaa aatgttggga
    12481 aaaatgatca gaatggaggg atggcttact cagaaacgat tgtacgtgat gcaatctata
    12541 aaaataagaa tgaaaaagta ttgtataaag ttgatccaat ttataaaggg aatgaattgg
    12601 ttcctcgtgg ttctcatgtc gaagctttct cagttaacga taatggaaaa acggtaaacc
    12661 ttaatgttta tgtatttaac aatcaaaaag gaatcaacat caactatcaa aatggtactt
    12721 gggtaagaga ttagaaattg agaaggtgaa gaattggaaa caataagcat caatggatta
    12781 gataaatcag ttgcaaatcg aataaaaaaa ggaaatggct actatcttct gaagaagggt
    12841 aaatattcag ttgctagaga gttagaaaca atgagtatcg tgtatgtagc taagaatcat
    12901 tttgacgtag aacaggctat ttttgatcca gattctgaaa tagggaaatg tgttgaatta
    12961 tcagagaaaa ctgttagtac aaaaggggcg atgatcattg agagagaaat gttagacgaa
    13021 atcagagtaa tcattcctaa agatacgcag atgtgtgagg acagcataag tgacgatgaa
    13081 ccaaaatcag aaagaaaata aggagatatt cagatgagcg aaaaatccat tgaattaaag
    13141 gaatataagc gaagcgatgg agaaaaatat tatcctgtag ccagtttttc taaatcagta
    13201 tatctggttg gacgtgcatt ggtagttggt gcaattggca tatatatttt gcattgggta
    13261 acaaaggttg tacaactgct tgtagatacc ttagtaagta ttcttgtctc gttaaatgat
    13321 gttaacggaa cagatgattt aggattaaaa gaaatgttta aagagttact caatgcgttt
    13381 tttcccaatt atttaagtta ttcatataat gtacaaagct tttttgcaaa gctacacttg
    13441 gtagacatct ctattttagt attagaagca ggaacggtat tctttgttgg atatttggtt
    13501 actttgatgt tcaggcatca ctatggagaa caaagaccat tttttgatga tatggaaagt
    13561 agaaggttga aaagagaagc tctagaagct atggatgtga agtatgatcc aaacaagatt
    13621 ataggagatg aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt agaatccgat
    13681 gtgctatcaa cgcttaaaaa tatcaaaatt tctgttcata caagacgtga tctaagtgtc
    13741 aatgaagata ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa gaaagttaga
    13801 gaaacagttt tacgacaaat tcaagattta gggtcgatcc tgacaagagc aacaagcgga
    13861 cgagccaaat ttggtgaaaa agaagatgaa gtttcaggcg attatttcat tttccgagca
    13921 tcagtaaata ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa agaaacggaa
    13981 agagcaacat cttcaatcgc agtttcaaat tcacaatttt cttttccaat caatctcttt
    14041 gccgataatt ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata tgccgaaact
    14101 caaaagcaat ctttagaact ttatctatct tctgctgacg tacctgcaag tctaacagat
    14161 atgcacgttg ggaatacatc tattgaatat ttgtttactt tatctcgtac cagaaaaatt
    14221 caactaagct caatgaaaga agaaattgaa acttacttgc atatatcagg tgtaatcgtg
    14281 cggatcaacg cagggcgtat tcaaattact ctaccattag gagaaaatga tcgtattcca
    14341 attgatgtac catcaatgat caatagcgtg ttttaaaaga catcacaaat agaaacagaa
    14401 aatacaaact accagttcac aaacagaaaa gtaccgagaa aagtgaggaa tagaaatggc
    14461 tatgaaatta ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta ttttgctaga
    14521 taaagaacct aatataaagc ttaatggaaa attcagtgtc aatatgttga acacaaaggc
    14581 taaagacccg acagatgcta tttttggaat taagcctgat ggcacaattg ctcatttttg
    14641 catcagcgaa atgccacaca tgttggttgc aggaacgaca ggatcaggga aatcagtatt
    14701 actgaatgaa attcttgtta cagcaatgtg tcactcaacg cctgatgaat taaaaatagg
    14761 aattgtagac ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt tcatgttggc
    14821 tgatccaatt acagatatgg atgaagctta tgattttttt gaatatcttg tgatcttgat
    14881 gcatgaacgt tacaaactga tggaaaaagc agggttacaa aacatcacgc tatataacga
    14941 atatgccgaa aaaaatggat tagatagatt tccctatgtg atcttattag ttgatgaata
    15001 ttcacagttg gttggaacac ataaagaagt agaaggtttg attgttcaat taggacaaat
    15061 ggcaagggca gcaggtattc atgttatctt agctacacaa tctcctcgat caacagttgt
    15121 cactggtatt atcaaagcca attttccgtc acgtgtttgc cttatggtag caagtgattt
    15181 agaaagtagg atcgtattag atgaaggcgg tggagaaagc ctaagtccaa agggagatat
    15241 gattattaag ctcgttaatg gaagtatggt tagagcgcag ggagcttaca tcagtaacaa
    15301 agaaattgaa acaattttca atcaccttag aaatactatg ccagaaccag aatatgttga
    15361 ttaccgagca atcgtagctg aatctcgtgg tgagggtgga gaagacgagg attttggtgg
    15421 aattggtaaa gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac ctttagcaaa
    15481 ttcatcatct aaagagccat caattgaaaa gaagagtacg tttgctctaa aacttgaaaa
    15541 aaacaaagaa gaaaaagaaa aacaaggagc taaaacacta gaaaaacttc aagccgaaaa
    15601 attagaaaaa gttttaaaag cattagataa agctgattct gaaccgcaaa cctcaaacaa
    15661 cacgaataat gaacagccga tttcatctaa aactaaaccg aaaactactg atccgaaagt
    15721 ttcagtaaac gtgaccatca caaccgataa gtctgagaag aaacaagtaa acggaacgac
    15781 ttcacctatc atgcaaaaaa tgttgaaagc caaaaaacag gtgcataaat gatcattgtc
    15841 agttcttttt tgaggtggct tacgccatat aaatatacag atgaagtagt tgtgaaacga
    15901 acgaagaaaa gagataaaaa agcggtacta actcctaaac agatgcttga tcatggcatt
    15961 ttaggggtga aggttttaga agtgaataaa ccgtttgaat caaatagtgc aagagaaaag
    16021 ttgcttggag ctgaagccga tgtcgcatat ttttaccatg acaaaagcaa gcatcaaata
    16081 gctattaaat tcaatccaaa atcggcatgg cggtatcaag caagagatga cttgaaacgt
    16141 aggaaactta gaccaatgct ctatccagaa ggaaaagaag catttgatag aacccacatc
    16201 gttcccattg gttttcatgg tacagaatca gataaccgcc tattagtagg ttggaacagc
    16261 aatcaaaata gagttcagat caataaattt gagaagaaag taaggaaatt caacgatcga
    16321 aaaacgatcc tatggtttgc agatatttgt aaacagccgg atggaacagc aaagtgggaa
    16381 gcggtgatat ttgacgaaaa tggtaaaaaa gtaatgtctg aaacatggca tgataaagct
    16441 aaatttgttt ggcagtagca gtagaggtga aaaagattgc tagaactttt tgaagcaaat
    16501 agaacaggct ttaatcaaaa gttagaagtg ggagaaacta tagaatataa agataaaaac
    16561 tatattatta tcggaatcta taacacgaga attaacagaa taggtgatcc acgtataacc
    16621 agtgacttgg tttgtcaatc tgttaactat tcacctgatc tgacaagcag gtataagaaa
    16681 tacgtgttat tagaatatag acacaagatt actgatgaaa ttggtgtaaa caacacacga
    16741 aacatattta agatcggaac agtgattcct tatagtaaca acgaagaaaa aatattttat
    16801 cagatttacg gaatagaaaa gtttgaatat gaacatgtcg atctgctagt gacgtaccaa
    16861 atgcgtttaa tagaaccttg gtcacaggca gaaattgata aagcagtaaa attgaaccga
    16921 ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa
    16981 ataccaaaaa attagcaatg aacgtaggta agaacatgac aataaataat aataacccta
    17041 aaaaatataa tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg
    17101 ctgatgaatt aggcaaatca aagagcctag tatcacacga ttttaagcac aatgtttttc
    17161 cgaacccgat tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa ataggtaagt
    17221 ggattgatga agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagca
    17281 aaaataaaat tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga
    17341 caaccaatga tttggctaaa ataaatcttc ataaacaaat tattgaagac tttgaaaaaa
    17401 atctttcaac aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga
    17461 ctctttttag agaatatgga gaatcggcag ttactaaagc aaaggaagta gcgaatgaga
    17521 gaaaaaaaca aaaatagtag tcgataccta gtagcgactg tagtttttgt ttgtgtattg
    17581 attttcctgt ttgatttttt gcagattgga aaaagcataa ttggaaatac attgagtgca
    17641 aaggaaatgg ttgctttagg tggtttgtat gaaggacact tgttcccgaa tatattcatt
    17701 tctatcgttt ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt
    17761 cttgaagaag cactgaatga tcagtatgag tggttaacat tattgatcgt tttaatcata
    17821 tcaggatttc taggaagctt agttcctttt ctgctacaca gcaacatcgt aagtatgggg
    17881 cttagtggag gtatttttgg atggattgct ttatttgcga ttgcatacat ccgttttgat
    17941 agcaagaaac atttattact aagtgttttt gtattattga taatcggcat tttactggta
    18001 tctacttttg aatcaggcga gacaaacgtc ttgtcacatg taatcggatt tgtctgtggg
    18061 atgatcttgg gatttattct aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga
    18121 gtgctaaaaa tgccaaaaaa aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa
    18181 atgttatcac ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga
    18241 aataaaaggg ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat
    18301 gatatacaca ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag caaattagga
    18361 ttagctcaac aacgaatatt tgaatggctg aatacgaata ctgagcaaga accgttaaga
    18421 gcaattagta atctttttga atttggaacg aagcattggt ttaacgctgt agaaaaggat
    18481 gctgacttac aagaagcgtt cgatacacta agcgaggata gcaaatccat gcgagactta
    18541 ttgatcactt atctaaatga gcatgaattt gaacagacga aagttaaaaa gaaaaataaa
    18601 aaatagaaag ttgtgatgta tcatgctgtt taccttatta ggttatttat gtttagttct
    18661 tgcttttttt ggagcaattg ctatatttgt cggtcagttt caagaaaata atccgataat
    18721 gcgtaacata ggcattggaa ttgttatcat atccttgatt gtatttggtg ccacaaagat
    18781 ttttgggagt aatgatcagc agtttggtat ttctggagtt gataatagtt atgctccaga
    18841 ttcattggga agagtagtga tagatggaac gtttgagaat gtaacgagct acaaaaatgt
    18901 ttcattgaca gtgaaagaag atgacaagaa agcatcagat gacgttctcg ctacgatcaa
    18961 gccgtctcaa tcacagtttg aagtgtttta tacagtcaaa gagaataacc ctgtacgcaa
    19021 gctaacatta gtttttaacg ttgacggaga agaatactat aaagatgtaa aaatcaataa
    19081 agcatcaacg aaaaagattg tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac
    19141 aactgaatca agttctactg tcgaagttga actaccagat aaaattgatt ttgatcctga
    19201 aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggca gatcggttta acactgaagt
    19261 tttcaaagct atcaaaactg ttaaattgga tactgaaaag caaaggatta atgttacctt
    19321 attcaatgca gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg
    19381 gaaaagagta attggattgt atcgaggtac ggcaaaacca gattcagacg gttcagattt
    19441 gccagttcac gtagtttatg aagacggtaa agtttttgga gactctacag caaatgaagc
    19501 actaacgatt aaattagcta aatagccgga aaattataat aaacaagtgg tgtcacacca
    19561 ccatcgcata ggtgtgacac cttggagatg attactgatg gtagtaaata aagaaaaagt
    19621 aagaaagtat gttactttaa aaatagcttc tgttgaatat ttagaaaagc gagctaaaga
    19681 agaagaaaaa acaagaggta agaaggtaac agtttcagag ttgttagaag aattaattga
    19741 gagtgaccaa acaataagac gtgcatttac caataggtct gttaaaagtt agggtgatac
    19801 tatctttaat ttcaaccaaa tttctaatat atgatgaata gtttccttgt acaaaatatc
    19861 taattaagag ataaaagtta ggaggattca ttcaaaatga ttatccaaaa gtttgactgg
    19921 aaaatgaaat taggaattag tcttgtaata ttgggattta ttattttttt ggggataaaa
    19981 gcacaaaaaa atcaatctac ttttgaaact atgtcttctc aaattattga ggaatgttcc
    20041 aaaatttatt attgtgagaa caaaactgaa gaatctaatg gcttttatta tatcgatcaa
    20101 ccacatgatg ttgttataaa aatggagtca catgaagtaa acgaatggat tcaaagaagt
    20161 actaatgtat caaaaaagtt agaaagatat tctcaaacgt ttttaagtaa agtcattttt
    20221 tcaaattgca aaaatgaaga aacgtctcaa agagtagcta gttctttaga aaatttaaaa
    20281 ttttctgttt caaaaaaaga gaaatttgta gatttacgaa aataaactat cgaaagtagt
    20341 atgattatga tcttaaaata tcatcttaag agaagaatta cgattgttta taggatttac
    20401 aaaatgaata agtttgaaga gtgatttggt cgcagactag atcactcttt ttgtgtaaaa
    20461 aaactttaca cttatttttg agtgtgttaa gattttttat atagagtgac ggtttcttgt
    20521 cagagtggat gataaccatc aatattggag gaataataag tggaaaacaa taatgatatt
    20581 agatatttta gcattgggga aagagttaca acagtaagtg aattatcaaa ggattataga
    20641 gataaagaag gaacgattat ttctcttaaa aagaatcgaa gaggtgtttg gcaatatgtt
    20701 gttgatttag gggatggcgt tacagttgag tgtaaagata gtcaactgat cagaaatgat
    20761 ggcagtcatt ccattgaaaa agcaattctt gtattcccaa aagtatcaag caagattatg
    20821 aatgactggt tgcttggaaa atttggtgag tacaataaaa aagaaatcgc agaagtgatc
    20881 ggcaaatctt atccaacagt attagggtat ctaaaaaaga taacgaacaa agaagcgaaa
    20941 gttgaggatg ttttaacagt aagagaactt cagtcattaa tcgaatatcg gaataaacaa
    21001 ataacaaatc ctgagtacct taaaaaagaa gatggtctat tgaaacaatt ggaacaggta
    21061 aataaaatcc ggaaaatgca aaaggtagct cctttaagcc tagataactt taaaaaaatc
    21121 tatttgaaag agatcgaaaa agactaatgt ggagtgtcac tttaccgttt caacctgtat
    21181 ctgcaccaag accaagtgca agaaggaatg ttgctgatgc aactaatgac gtagcagaga
    21241 aaaggatttc aacctattat gataaaaagt atctcgatta tttgaaaaaa ataaaaagct
    21301 ttatcgaaga tcaaggtttg ttagatgatg aattttactc gatcgtaaat gatccgatgg
    21361 gtgcaattat ggaagtggac ttttactatc agataccaaa aagctataaa aaagtctata
    21421 acattatgaa aacgacagcg ccagatttag ataatttagt aaaaagtgct atggatggta
    21481 ttttcaacat ctcagctata cgagatagcc atatcacagg gttaattgct tttaaataca
    21541 atgtggcaaa cgaaggaaaa acagttgtga ggatcaagcg atattcagaa tttgagtggg
    21601 atacttctga aggattgaac ggagatattt gggaagccac atttgatttc aaaccagtag
    21661 caacgccaag accaaagtct aaatttagag gaaatgggat tatcacttac tatccaaaag
    21721 agtataacga ctatttggaa aatatcaaga atacgcttaa agagaaagat ttggtaaatg
    21781 atcaactgaa aaaggtaatg aatgctccta tgggggttat agctcagata gattattttt
    21841 accaagtgag aaaagataag aaaaagcttg attcactgat gcgtacttca cagccggata
    21901 ttgataactt agtcaaagga acattggacg gaatttttaa caaggaaatt ggcatcaaag
    21961 atagcagggt ggtaggttta atagctttta aatacaatac ttttgaaaag tcgaaaacaa
    22021 acgtgcgact acaggagatg ggctgatgaa taataacgaa aaaattcttc atgtacttga
    22081 tagctttgaa acaattcaag aggaactaaa gaaatatcga gatgttttgg aacaaagata
    22141 tgattttgtc aatcagcaaa agagtaatca catggatttc attctcaata tgaatgactt
    22201 gaagaagaaa ttagttgaac gaaaagaaca agaaaagttg atcaaagctt actttgaact
    22261 cggtgaaaaa gaagttaaaa aggcaatgga actgaatgaa gatcgcaggg ttttagatca
    22321 gctgttagaa caattattgg tgatgtttca aaaaggacga atagatgagg atttaattga
    22381 agaagggttg agaaagtatc ctgcaaactc aggtatcgga atagtgctga aagcgattga
    22441 tgaagaagaa attgaagatt tcataccggc agaggatttt gaatcggcaa tggaatatat
    22501 aaaatattac agccaaggga tcactgcttt tagagagttt gatcctgaag atgttattca
    22561 tgacttgaac aacctaaaag aatggtgtga aggttatgaa gttgatgatt ccggtttgga
    22621 ctacttgatc agtattatgg aaatcgaaga agagatgccg gataagcctg atccaacaga
    22681 tattttagaa ttaatccatg atgctagaaa tccaattgca tacatttcta gaggatatac
    22741 tgtattggaa tactataagc cttatatatc agcgatgaac catttaagac gtgtattaag
    22801 ggaaaaacgt gaataccgct cagtgttaaa tgcatcgaat agattagaaa aagctgtttc
    22861 agagctagat gcttattatc gtgaacatta cctgcaagca ggtggtatgc caagaaatac
    22921 gaaagcaaat atcagtaggt atataaaaga agctgagtag gcggagttag cccataaaat
    22981 ggcaaaaggt aacctgaaag aggcgttttg aatgaaaagg gatgtttttg aattgtacgt
    23041 attagaacgt ctagaagacg gcatgatggc acaagagata attgatgaaa ttgatgatat
    23101 gatcagcaga tttggtcaat tggaattgaa tgatggaaac aaaaattcgg tagtgctaca
    23161 acaattttgg ttaaataatt tttcgtataa ggattttatc aaagaaaagg caaatagatt
    23221 gtactgacta aattcacgag gatcagtgat gagagcttat tgctaagagc agttaggctc
    23281 ttttttgttt gtgttaaatt attttatttt acaatacaca tttatacaca aaaaatgttg
    23341 acggatagta cacaaatgtg gtaggatata tttgtaagca agttagatta acaagttaaa
    23401 ggaaggaaga gcagttatga aaaaagaaga actaatagat atgtttcaaa ttgttgaacg
    23461 agcaaataat atgggaatta tgttttttga tcgtatttct ttaaagatgg atttaagcgt
    23521 tgcccaccaa gaatttaatc tacgattaaa agctttattg atttctgatg atgttaattt
    23581 cgctcatgat gttgtaggta tacagaacca tatagataga gaaaacaaac gtatgggtga
    23641 tggattctta ccaagatatt caagtttatg atttggcgaa agattcaaga gtatgtgtca
    23701 ttattgatag gtactcttga attggaaagg aggaatgaat ttaagacaac aacgattatg
    23761 atcttaaaac aatcgtttat gtaattggca atcttacgaa taattttcat gcaataatga
    23821 caataaaaaa tgaaaagggg taaaatcaaa tgaaaaaatt tttagtagga attctattca
    23881 caatggggct agtgctatta tcaccttcag tttcagcaga aagctctatt tccatgtatc
    23941 gtatgtataa tcccaatagc ggagagcatc tatacactag aagcgttgga gaaagagaca
    24001 atctgaaacg ttatggatgg cattacgaag gtgtcgcgtg gcaagctcca acaagtggtt
    24061 ccccagttta tcgcttgtac aacagatata acggtgaaca tttttacacc gttgatgccg
    24121 gagaaagaga taagatttcg cacacaggat ggacgtacga aggtgttgcg ttctattcct
    24181 acaacggagc aaatagagtt cctgtgacac gcttgtacaa caaatacgtg aattggcacc
    24241 attacacgtt agatcaaaat gaaaagaacg taattagtaa acaaggttgg aagattgaag
    24301 gtacgggttg gtatgcaatg ccaaacagcg caccaaatcc tacacctgca cctgctccaa
    24361 gtaatgaaaa agtgttaaat gttcctgtca tttggcaagg taatacgatg ttgtgtgaag
    24421 gtgcttccct gttagaggga ttgcactaca aaggagtagc gacaaatcaa aacttaacca
    24481 gttttgtaaa ctatatgcca agagctaatg acaataatcc atatcatggc tattctggcg
    24541 aatggcgcta caacgtaaat ggtacgtatc aaggcatgat gcctgatcca gtagttaaat
    24601 gggcaaaaaa tgttggtggt aatgccgtta atatcactgg ttcaggaacg aatgggatca
    24661 agaatgaaat tcgcaatggt aatccggttg taacttggat tacttataac tatgcttcac
    24721 ctcaattcaa acaaatgcct tggggacgtg cagtctggaa tggacatgtg gttctcgtgg
    24781 atggcttcaa aaatggtgca taccatgttg ttgaccctgt atttggcgca aaatggatca
    24841 atgcaagcac gtatgagaga gcatttaatg tgactggaat ggcggttgct gtacgttaat
    24901 tgatagaatt aataaataaa tgaaataaga gcttaattac tgaaatggta aatgggctct
    24961 tattttttag aaaaaaactg ttttttgtaa tacgaattta taacaaaaaa cgttgaatgt
    25021 agtacacaaa agtggtatat taaaaccgta gagattatgc gaagaggtga ttttgatgaa
    25081 ccaaagaaga agaaaaaggc tagaagaaat aagcgagcga ctatccgagt taaaggatga
    25141 gttagaagta attcaagaag aagaggaaga gtacaaagaa aatatacctg aaaatctaca
    25201 gggatcagaa agatatgaaa aagcagaaga atccgttgat caacttgaag aagctgttga
    25261 tggtttatcc gacatattag aattgattga agatatagct agctagagat gattaatgaa
    25321 gatttcagga gtgagcaaaa aatgattgat atttcactga tagagaagct acttttttca
    25381 aataagtaca cagccaaaga gttggctaca agatggcaaa tatccgaatc agctattaaa
    25441 gcctatcgtc aaaataaggg aagcgtagga tggcgtgatt ggaaaggaat cagcattgga
    25501 aaagcagaag acataatgaa acgtgaagga tttcaagctg acaatgattc taaaaaagtt
    25561 gaatagttta atagtcgcca agaaaagtac caggacgagg ggaaaggtag attacaaagg
    25621 aaaacacttt taaataaact tcgcaaaagc atcaggttat gagtggctag ggctatacga
    25681 atgtgatgaa aacaaagggc tttaatgtca aagaaaacgc aatagggggg gaacaaaatt
    25741 tttaaaaaag atatttatgt ttggtttaat ggaaaaaaat atatgactac taacaaaagg
    25801 tggtcagagc tagtacaaaa tacagttaaa aaagtcatgc cagatggata tcatttcaac
    25861 tatatacaat gctcacaaac aactgaaagt atttattttt caatattaaa tgagaataga
    25921 ctctattatt tcagggtaag ttcacaccat aggaaaaaga gagaagataa tgttacgaca
    25981 tttttatcgg gaaaatacaa tggcttcaaa gagttaagtg aagccattgg tgagtatcta
    26041 aaaagtaata gatttgtcag aatttctgac aaacatatat tgatcttgtt actttatttg
    26101 aataatagct tcaaattgtt ttggagagac gacactttct actatattga gaacggcatg
    26161 gatatttatt ctggtatcaa cggcatatta aaacattata ttaaggcgtt acttcgatat
    26221 ggggttcttt atcaagaaga tttaagtaac caaattaaga ttagtaattc tggaaagtca
    26281 ttactggcaa aatataaaaa attgctttct cctatctaca aaggaataga tatggaagct
    26341 gtaaattctg aaactttatt taatttagtt gttgatgaaa agtttgcgaa tgaatttttt
    26401 aaaagtaaaa aaattgaaag actaaataac ctgtttcctg aaaatgcaat aaaagcttat
    26461 gtagataata ataaatcacg attatacaac tggtttattc ccaaacggtt gcgaggaaaa
    26521 atcaaaattg gcgatcaagt gaaagtaatt aatctaaatc atactaagat cgttaccgta
    26581 acggagttgt atatagctga aaaagaagat attacaaaaa tgaaaccagt tattaaaata
    26641 ctagataaca aagagtgagt ggaatatatg gtagcaaaaa agaatactct aaaagaagct
    26701 tctgaaaaat atcaagatat gaaaagacga ggattaatac caaaggacaa aaaaggtata
    26761 tggagagaat ttgttaagga agtcagtagt gatcgaagtg gcggtgactt tgttggacga
    26821 tatacagcaa aaggttatag caggcgctta aggatcactg ttgaatatgg aacgaatgaa
    26881 tcagctacag agctgaagca aaggtactta gatacacgaa gagaagcttc aaaaaaatat
    26941 gatgatttca agagcagagg tctaattcct aagaagaaaa aggggatttg gcgtgagttt
    27001 ttcaaagaag aagatagcaa tcggaataag ggagacttca ttggacgata cacagcaaaa
    27061 ggctacacta agaatttagg gatcaaggtt gaatacggaa ctggtgaaag ttcatgggaa
    27121 ttgagaaatc gctatttaaa tacattgtta gagaaatata aagcagaatt attggagttg
    27181 ttcgatgtta atgattttat cgagatggct gaaaagtatg gtcatgaaga aggttttatg
    27241 atcaatgaca ttatggttat tgataatttt acggatttat tagcacttgc gaaaaaagca
    27301 agggatttaa gagaaacgca gatgtatcta ttcagaaata aaaacggaag aaaccggaga
    27361 actgaatcga gtgagattga agattactat gatgccttta cacacgatct gggagccttt
    27421 gtagacgaga taagtggtgt aatgaacaaa tatagggtct agaataaaaa agccggagat
    27481 gagcagataa atgggaaaca atagcatata cacacctgaa atgcagaata aaattaaaag
    27541 acttgtcgga ttagggtgga tgacaagagg aaaattagat tcttttagta cgatcaaata
    27601 tcttttgatg gacatttacc gtcagaacaa gcaaacagga atacattttt tgggctatga
    27661 taagccggaa gatgcacctt tctttgttcc aagttcagcc tatagagcca gtacctccgg
    27721 tagcatgtat aacttggatt tgtttgagga aggagcaaag atgatcgaat atgatttcaa
    27781 cgaagcctat tcgaggatca tgagaaatta tcctttgccg tcaaacactt atttgaaagc
    27841 aaaatataat acagatcaag ctattttgag agcgataaat agaccaaaag taagaccgta
    27901 tgaaaattta aaaacgtata tgttcttttt ggttaatttt gaaggaacag ccaaagaagg
    27961 aacatatgct aatccaaaat cacagtcgat gtttgcggat tatggcgaaa atcccaaaac
    28021 agctaatgag cctatttggt tatcggagat tgagctaaca cttatccttg attattatga
    28081 tattaaagag atggaagtcc tagatagcca cacatttttg acgaaaaaag gattactcaa
    28141 agaatatttt gaaagaatcg agccattaaa aactgatcct gatacgttga agctttataa
    28201 aaatcttaga acgaaagtat ttggtgctat cggacaaaaa tcattaagag aaggagaaac
    28261 aagcaccttc gataaataca gtatgtataa tcgagcgtat tcctccatgg tagcaggcgt
    28321 atttagagat cgtatggtta gatatgaaca gaaatacgtt catagcgcat atgggctatt
    28381 aaaaattcgt actgatggat tgtatttcag atccgaagta ccagagtttg aagagctgta
    28441 taaacttgga gtagttaaga aaaaagttca tacaatatcc aaagaagaag tcgaaaaagc
    28501 tttagaattt aaaaacaaag gatttgaaaa taagggctcc tttgggaaat ctaaagatga
    28561 agttaagggt gttgtgatag acgatgattt cgagtttgat tttgactaaa tactctttgg
    28621 aaaagatggt aggaggtaaa tatgaagaag aaaattgtgg aaggaaatgt aggtgttatt
    28681 attctaaaag attcaactga ctatgctaaa tcaatggcac taaaagacga aaaagttgca
    28741 cgcatggata aagatttagt tgaatttctg atggatgata cagaaagatt aaaaggggat
    28801 cagtttttga gtttaatgaa agacaaaggc ttaaacacat atggtttatt tgattataag
    28861 aaattagcta tagagtgggt aaaagaagga gaaatatttt ttattgaaga atgggaaggt
    28921 actgagaact tagttcttca tcagtatctt ttgaaagctt aatctaagta aaacagttct
    28981 gactagttat gcttgtcaga atttcaaagg aagttttcca taaccatcat cattcaaaaa
    29041 taaaggattt gaaaataagg gttcctttgg gaaatctaaa gatgaagtta agggtgttgt
    29101 gatagacgat gatttcaagt ttgattttga ctagattttt ttgcaatgtt taagagtaaa
    29161 caaggtaaga actaagatca tgaagattga gagtaaagaa gatagaggac gaatgtatga
    29221 gaacacacta tatttattta gaagataaaa ttgtagatga aattccggca aaaagtcgag
    29281 aacaagctag aaaattattt attgaaaaat acaaagagat caatggagat agtgaagttc
    29341 caaaagtggt cgtaacagtt agaaaaaggc aaacaaaaaa gagaagtacg tttcatgtca
    29401 gccctgatat atataatctt atagagcgac atagaagtga agataatatt tctatctctc
    29461 aatatattgc tacagttatt gatgaattga tttcaggaaa gtatgactat gcttttgatt
    29521 ttgagggtga aaaggattac aaaagtgttg ctgtctttca atctgatttt gagaagctaa
    29581 aaaactatgc tggcaggaga aatgttaatt ttgcaagact actagaagag ttctactccg
    29641 aagagttggg agtaggaaat gaatacaaga gagaaactta tgcgaaattg tctcataagt
    29701 tatcccaagc gcttaaagaa gtgacagaag ttaaagcgga actagatgct aaacttgaag
    29761 aagtgaaaaa atatcaaaca gagaggaaga acaacatgaa gaatcaagtg gaatttgacg
    29821 aacagaaaga atttgaaaaa gcaagaaaac gctataacaa aattatgaat gaacttagct
    29881 atgaacactt aacagttcat gaataattga gtgagggtac agaaaaatat acgatcgcag
    29941 atatggttaa tgaagtggaa actcttttag cttctttctt tgaaaatggt cacgtaaacg
    30001 atgaatggcg aaaagaagaa cctaaaaggg ttaacgctta tattgctcga ttaaaaaggt
    30061 ttattgataa atataaggag aatagtgaca aattttctaa agataaataa ggagagagtg
    30121 tgattacatt tgccagaaat cgaaggaata ttttcgccaa aaataggtaa aaaacagtta
    30181 gatttcagca gttttccgag cgtaaataga gattataatc taggtgatga ggtacagata
    30241 ctaaatattg atttcaaaga actagacttc gctgttgctg tttccaacaa acccaaggca
    30301 ttggaacgag tgaaaaaaat ataccaagag aatgatcaaa atatcaaaaa tatgatcaaa
    30361 aagtatagac gagataattc aaaagttgaa gacgttttat tcaacggtac ggcaaaacat
    30421 gatttgaatg tttatatggc attaggagtg gctttgtact gtaattcaat taatcagaca
    30481 gatattttgg ttaatatcgt gaggaatagc tatcaaaaaa tattaaaaat aagtagcgaa
    30541 acaaaccctt attatgcaat gaaatatctt ggggataatt tattaaatga taaaaaattg
    30601 atgcacaaca ctgaagagct gttatctaat ttggctgcga ttaactttct attcaaaaca
    30661 ggttctgaag attattttat agataacgta ctaaaaggtg cttacggaac agcattttat
    30721 ccgctttaca atatgaaaca gcataaagcc gaacaaaagg ataacaaact tgaaattaaa
    30781 aagatgaaag aactattagg tataggattt gtaaaaccca attcaaaagt cactattgct
    30841 tcgttagcaa ctactaacaa tgatgggaaa atagtaaatg ggtttgacga ttcagatata
    30901 gatatattgt caaaatcata tttttctttg tatggagatt ttgcagattt gatgggtatt
    30961 gatagttact tagtcaataa ttttgagatc aagggtagcg agctgaatga tattgtgtat
    31021 ttcgttttaa tctctctata tgcaattgat cagaataccg agtaccgaca aattgactat
    31081 ttgctagttt tttatagttt gttatatcag ttcgttttaa ctcggttgtt taaaaatgct
    31141 aaggaagaat tgatcctgaa ttctgattat gaaatagcaa aacaaattgc aagtattaga
    31201 aaagaaaaca aatctgaagt aatcaaatta agaaaagaat taagtattgc gagtaaagaa
    31261 aacggtcggt tagaatcttt agtcaaatcg gcaaaaaaag aagaggaact tttgaagcaa
    31321 gagattaaag aattaaaagg aatcagaaaa gaacttgaac agctgaggtt tgaaaataaa
    31381 gtattaaagc aattgcagga gcaagaacat agacaatcat taaatggtaa aactgaggag
    31441 ccttcactgt ctgaaatgat agcttatcta aaacataaaa acattctttt agtgggagga
    31501 catgtaaatt ttcaaaataa attaactgaa ctattaccaa atattaaaat gtttgatgta
    31561 agcgaaaata atggaaagat acttaaaaat ttgaatacgt gtgatgcgat ttttctatat
    31621 acagattact tgaatcatgg tctatattat cgtgttatga gtgaaatttc taataaagat
    31681 aagatatttt atttggataa tcatgtgaat atgaacttaa caattaaacg tatttacgat
    31741 cttttatcac ttatataaag agagaagtga taaataggac acaaaatttg gaaagtaggt
    31801 aagtcactat gagagaacta actttatctt ctaaggaatt agaaaagaaa actgctttat
    31861 taaacgaaat atttagtgct gattgtataa tccaaagttt aaaagaaagt tcattttcaa
    31921 aactaacaga tcaggaaaaa atttcttata tgctaagttt agataatcta ggaactaaat
    31981 atgatgaatc agcaagtagc gataagcttt tgattgaatt agtatcaagt cttagtagtt
    32041 ttggaaaaat gaaaagattt aatgtgatat tagaaatttt tgcttggtta atcccttcat
    32101 ttatttggtt tttttatatt ttcatagttg aatctgagtt aatagccaag gtctctcttg
    32161 gactgttaat tccgatataa ataggttcag caatttactt taataacttg tatacacgta
    32221 ttcaaataaa aaagcagtta ttacatgaag gattaagtgg taatatgtta aaaagaattg
    32281 catgggataa agataataaa acagatattg agcttaaaga atataaatac aattatttct
    32341 cgagatgtat agatagaaag ttactgttta aaaaagaagg attagaattt agttttgatg
    32401 aagatgaatt tgaaaaatta gtgatggact ggcgtaagaa tttactggat acggtacaat
    32461 tctaaaagca attgaggtcg aaattccgat acttgaatca tgatctatat tatcgtgtta
    32521 tgagtgaaat ttctaataaa gataagatat ttcatttgga cagtcatgtg aatatgagct
    32581 taacaattaa acgtatttat gatcttttat atgtttataa acattaatga ggtgctaaaa
    32641 ttgacaacaa taactatgac aaaaaaaata gaagtatatg ttgatggatc acaagaagaa
    32701 agggattcct tttttgattt tttttggaaa aatagtagag aatatgtaaa agcgttgaat
    32761 ctggtatata aacagctaat gtttgaagag ttgataaaag atgcaattaa agaaatggat
    32821 caaggttatg ctgagagaga agaaagtatt caaaatcgga ttgaaaagtt agcatatact
    32881 gatccaatag atcaaaaaaa acttgataaa gcaaaagctg aattatctaa attgagaaat
    32941 gaaaaaaata aagaagcgag agaaatatta acgcaagcga tagggttgaa gagtcaaacg
    33001 agaacacgag atgtagcaaa aaaattagac tttgactttg gagattttgt tgattccgca
    33061 aatatgaaag ctagtaaaga ttttggcaat gattttatcg gaattgtgac cggacaaaga
    33121 acgcctagaa tatataaaaa tagaaacaaa tatggattac taccttttag aggtcgagat
    33181 attaaaatcc ataaagatga taagttttat atttatttac ctaaaggctt taggtttaaa
    33241 gtgaatttag gtagcaagcc aagaaaagca agagatgttg cttcagtttt acaacacatt
    33301 atggatggcg attacaaatt aaatcaaagt aatcttattt ttgatggaag aaaattattt
    33361 tttaatctaa caatttcttt tgaaaaaagc tataatgaaa tggaattcaa gaaaggtaat
    33421 gaattgatcg taaactttgc agatttgtgt agcggtgaat gtttaattaa tgataaatct
    33481 gtaatgtctt ttggtgatag ccaatatata agaaac
//
LOCUS       DAFFFQ010000022        31159 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000022
VERSION     DAFFFQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.022"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            159..395
                     /locus_tag="LQB69_001161"
     CDS             159..395
                     /locus_tag="LQB69_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3525337.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            392..790
                     /locus_tag="LQB69_001162"
     CDS             392..790
                     /locus_tag="LQB69_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL3525338.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1457..2401)
                     /locus_tag="LQB69_001163"
     CDS             complement(1457..2401)
                     /locus_tag="LQB69_001163"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL3525339.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(2697..3641)
                     /locus_tag="LQB69_001164"
     CDS             complement(2697..3641)
                     /locus_tag="LQB69_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525340.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            3862..5076
                     /locus_tag="LQB69_001165"
     CDS             3862..5076
                     /locus_tag="LQB69_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3525341.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            5098..7113
                     /locus_tag="LQB69_001166"
     CDS             5098..7113
                     /locus_tag="LQB69_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL3525342.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7196..8569)
                     /locus_tag="LQB69_001167"
     CDS             complement(7196..8569)
                     /locus_tag="LQB69_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL3525343.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(8595..8879)
                     /locus_tag="LQB69_001168"
     CDS             complement(8595..8879)
                     /locus_tag="LQB69_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3525344.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8881..10896)
                     /locus_tag="LQB69_001169"
     CDS             complement(8881..10896)
                     /locus_tag="LQB69_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3525345.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(11075..11380)
                     /locus_tag="LQB69_001170"
     CDS             complement(11075..11380)
                     /locus_tag="LQB69_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL3525346.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(11469..12500)
                     /locus_tag="LQB69_001171"
     CDS             complement(11469..12500)
                     /locus_tag="LQB69_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3525347.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            12707..13582
                     /locus_tag="LQB69_001172"
     CDS             12707..13582
                     /locus_tag="LQB69_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3525348.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(13734..14576)
                     /locus_tag="LQB69_001173"
     CDS             complement(13734..14576)
                     /locus_tag="LQB69_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3525349.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(14668..16545)
                     /locus_tag="LQB69_001174"
     CDS             complement(14668..16545)
                     /locus_tag="LQB69_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3525350.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(16542..17963)
                     /locus_tag="LQB69_001175"
     CDS             complement(16542..17963)
                     /locus_tag="LQB69_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3525351.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17966..21355)
                     /locus_tag="LQB69_001176"
     CDS             complement(17966..21355)
                     /locus_tag="LQB69_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL3525352.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            21612..23003
                     /locus_tag="LQB69_001177"
     CDS             21612..23003
                     /locus_tag="LQB69_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525353.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(23076..23723)
                     /locus_tag="LQB69_001178"
     CDS             complement(23076..23723)
                     /locus_tag="LQB69_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL3525354.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(23731..24417)
                     /locus_tag="LQB69_001179"
     CDS             complement(23731..24417)
                     /locus_tag="LQB69_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525355.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24423..25496)
                     /locus_tag="LQB69_001180"
     CDS             complement(24423..25496)
                     /locus_tag="LQB69_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525356.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            25718..26392
                     /locus_tag="LQB69_001181"
     CDS             25718..26392
                     /locus_tag="LQB69_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3525357.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26397..27737
                     /locus_tag="LQB69_001182"
     CDS             26397..27737
                     /locus_tag="LQB69_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3525358.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27886..28872)
                     /locus_tag="LQB69_001183"
     CDS             complement(27886..28872)
                     /locus_tag="LQB69_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL3525359.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            29596..30627
                     /locus_tag="LQB69_001184"
     CDS             29596..30627
                     /locus_tag="LQB69_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3525360.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            30736..>31159
                     /locus_tag="LQB69_001185"
     CDS             30736..>31159
                     /locus_tag="LQB69_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL3525361.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         9321 a         5775 c         5583 g        10480 t
ORIGIN      
        1 aatacgagag tttgtgacaa aagtcatgtc acaaactttt ttctttgtca ttttttcgta
       61 agaaaaagca aaataaaagt atatacattg attaaaagta tatacttgga tataatgtac
      121 ttactataga atgagaatgg tatagagggt ggcgaaatat gaaaaaaatg aaaaagtata
      181 aaattagaaa atctggcaat agcgatgtaa caactatccc accagaagta aaagaattca
      241 tgggtgtgca gacaggtgac gcaatcagtt atgtttttca atctgatggt tctgtacgaa
      301 tgatcaaagc acaagaagaa cctgatattg attcattagt tgattcgatc atgaatcaat
      361 atgaagacgc gttaaaagat ttggttgatt tatgaggtat ctttcgacta aagaaattat
      421 caaaatcaac gcgaaagtta ttttacgtca ctcgccaggc gaaatgatcg gtgtaaaaga
      481 tgcaaatgca ttagatatgg ccgtcaaaca gccttcacaa gcagtattca atcaagaact
      541 ctatcctgaa atctatgaaa aagcggcgat tttagcaatt aatttagcta aaaaacatcc
      601 ttttcataat ggcaataaac gaactgctct tgttgccatg ttgttgtttc ttcaattgaa
      661 taattgtcca gttgcttttt ctcgtcaaga agctgttgat tttatcataa tgattaccac
      721 tagttcattg gatttcgatt cactaaagtc caagataacc agttatttac gacaaaccgc
      781 aataaaatga acgaattaaa aactttattt ctcaattaga tcagcatgtc attaaggaat
      841 atgctgattt ttttatgttt aatcatacaa aatagttttt tcataaaaat atggaaaata
      901 tggattaatt ttgaaataat tgatttaaaa ggaatacaaa agagtgaata gtaggaaata
      961 gtggtttaga actagtgggc tgatttttta tatattttag tttacataat atatattata
     1021 caaagtagat ttaaaaaatg ataaacctct ctcgaacggt tttttatcaa aaatcgtcat
     1081 ttttccatca aaatctgctt atgcaaaatt ataaaaaaat tttgcagttt ttcttacttt
     1141 ttttgtcgtt taattgaatg ataaaaacca gtttctttat tcagttaatc aaatatgaac
     1201 gaattagata tgaatcattg tatttgccgt tgcggaagga ttttttaatt aaccacagat
     1261 aaacattgat aacgattcgc ttattgtaat caacagatta aatttcatat gttcatatgt
     1321 acggatacat tttcggaccg attaatttta accattctgt ttatttgctt acctattaga
     1381 tatcaataaa aaactgctta acagctattt cagctgttaa gcagttttag gatttttagt
     1441 tcatattcat tgtttcttat aatacagtgt tcaatacttc tcttaaagca cttgttgatt
     1501 tgctgaacat tgctttttct tcttcgttga ttgataattc aacaacttca cgaacaccat
     1561 tttcgtcaac gattgatggc acacctgtaa agatatcttt ttcgtcgtat tcaccagtta
     1621 aataagcaga tacaggcaat acagcttgtt cgttattcaa gatagctttt acgatacgtg
     1681 cagtactcat accaatacca tagtaagttg cttttttacg atcaatgatc tcataagcag
     1741 cattacggac tttatcagca atcacatcta attcatcttg ggcaatacga tggtcttttt
     1801 ctacaatttc aaacactggt ttaccaccga cagttgtatg tgaccatgca gcaacttctg
     1861 aatcaccatg ttcacctaag atatagccgt ggacgctgcg tgggtcaact tttaatttca
     1921 atgcgatttc tttacggaaa cgagttgtat ccaatgttgt tccagtaccg ataacacgtg
     1981 atgttggcag accagattcg ttccaagcga tatatgtcaa aatatctact gggtttgaag
     2041 caacaacgat aataccgtcg aatcctgatc ccatgatttc ttttacgatt tggcgcatga
     2101 ttgacgcatt tgttttaact aaatctaaac gagattgacc aggtttttgg ttgataccag
     2161 ctgtgataac aacgatatca gcatctttac attcttcata gccacctgac catacagcca
     2221 cattttcatc gccccaagcc ataccatcta aaaggtccaa tgcttcccct tctgcttttt
     2281 cttgattcac atcgatcaat actaattcat tagagattcc ttggttgatc attgcataag
     2341 cgatacttgt tccaacaaaa cctgtaccga cgattactac tttacgactt gtttttttca
     2401 tgaataaaca gctccttttc ataactattt gttactttca tgaaaagtat agtcttgttt
     2461 attcatttta tcaacatgtt aattataata gttgttatta taatttttga tggaaacgaa
     2521 ttcctgaata cgcataacag ataaatgaca aatgtttaca cctatatccc ctgataatac
     2581 aaccaaaagt ttagaactga aaataaatcc aaaaaaattc gctgcaacga tataataaga
     2641 cagcaatcgc acctaaaaca attttccttc atatgcttta gtgaaagatt ttcttcttaa
     2701 tgtttatttc tccattcatt gggagtacat gttgaatact tcttgaataa tttgaataaa
     2761 gaagtcgttt gtttatatcc gatacgatta gcgatttctt ccaacgaaaa actcgtttct
     2821 tgaatcagtt ccaacgcaaa gtcgaatttt ttctttagaa ccagttcttg aaaggaagca
     2881 tcaaaataca ttttcaattt attactaaga tagaccggat tgtagccaaa atgagctgct
     2941 gtttctttta acgtacaaga agtaaaatgc ttatcgatat aatctaatac tctaagcatc
     3001 tcattttctg cgcatccatc tgctgatgaa aagctttgtt gttctaataa atgagtcaat
     3061 tcgatcagta atagtgatgt atagagattt aacgaacgga tcttatgact gtatgtatca
     3121 aaataaatca acagcatatt ttcaatcaaa tgcgtcaaac gacttgtttt tccgccgcga
     3181 aaatgaatga attgtgtatg ttcccctttt tcttttgatg catttatcaa aaaagaagaa
     3241 agtagattat ttgtgcttgc caaatgattc aatatctctg tagataaggt ttcttctttt
     3301 aataagatgt tgattaaaat atcctcttgc cctgtgtatt cgattctttg agtagcttct
     3361 ttatcaatga tcaccacgtc actttcttcc atctctatcc tttctccatt aataatttgt
     3421 aaacatgtac cgctatacat ataattgatt tcaacaaaat catgtgtatg ctcgggaact
     3481 tccgcatatc gacggtgttt actgatatga atatttccct gttgaaatag atcccctcgg
     3541 tggatttttg gcggtgtctg aagatggtaa gctaagttta aatcattgat attcattcct
     3601 tgttcacgct gcagagtttc gattgattct tcgttacgca aaaaaaatgt ctaattcagc
     3661 tagttccaat tgactccctc ctttttgtaa gcggtttctt tcgcttatta gttcacgctg
     3721 tactgctatt atatgcaaag ctatttttgg tttcataaag actttctatg gttgggacat
     3781 gaacttcctg tgattccgct attgagagaa taaccacttc atactatatt tttgataagc
     3841 gcttacaaaa atgaaaggaa catgcttatg aataaaaaga tgatgaaatt ttctctgctg
     3901 tcagtctctc ttattttgac ttctgctggt gcagtcacgt ctgcaattcc cagtatgtca
     3961 gctacttatg ccacgcgttc tttatctgct gttgaattat taacgactgc tccctctttt
     4021 atggtcacta tttttgtttt gattagtagt tttattgcta ggaaaatcgg acaaaaacaa
     4081 actgtgatca tcggattagt gattgcatcg atttgtggta tcctccccct cttctcacaa
     4141 agttacagta ttgtcttagc ttcaagagct ggactaggta ttggctttgg attgatcaat
     4201 tctttggcag tcagcatgat tgctgccttt tatcacggag atgaacgagc ctctatgatt
     4261 ggttttcaaa gtgcatttca aggatttggt gcagctttaa tgacatttgt tgcaggacag
     4321 ctagtacagt tcggttggca atataccttt ttagtctacg ccatcagctt acctattctt
     4381 gtcttgtttg ttttgtttgt tcctacacct gaaacaactg aacttcaatc gcatggcaca
     4441 gccccaacaa gacaagtaat caaaacgaat cctaaaatga ttagttatgg tatttttctc
     4501 ttgctggtca tcgtggtata taatgctgta tcgattaaat tagccacagt cctgacgact
     4561 tattctctag gaaacgaaac taatgcagcg aatatcttaa caatcatgca attggcaagt
     4621 atggctgccg gagtttgttt tggctggctt cagacaaaag cacatcgcta tatgcttaca
     4681 ctttctttac tactgatggg aatcggcttt gtattgattg ccactgtgat aaacctgtat
     4741 gtgattgggt ttggtgcgat cctaacagga atttcttttt cgttattcat tccttactta
     4801 ttcaaccaag taagtatcca agcgcccaag tacgcggaga gtttcaatac ttcaatctta
     4861 ttggttggcg ctaatttagg tactttcatt gcaccatatg gtttgctctt tctctctttt
     4921 gcttctcttg ggagtaaaac acttgctgtt ttcgtcaatg gcagtatctt gctgttgatt
     4981 agcgcgctta ttagccttct agtagtacac aggacgaata gaaagacagt gataagagta
     5041 gatgaagaaa taaaagaaca attgacaaca aaataaattt gatggaggtc gtgtaaaatg
     5101 acaaatgaaa aacataaaga tagatggctt tggtatcctg gagactttga gatccgccat
     5161 ggactgctac aaaatttcca acgggaagaa cgaggttttg actggccagc gtactggtac
     5221 atggatgatt gccatcggaa cgtcaagttt aagagatatt actttcttga tcaaccttcc
     5281 atgtttaaag tgacgatcca aggagttggg tatgtagaaa ttaatggaca aaaacatcca
     5341 tgtggaaaat ggctgacttg tccagctggt aaagcaaaaa ttcgtatctt tgtaggacat
     5401 acatcagggc taccagcgat gtttatcgag ggagacgaag taaaatcaga tattggctgg
     5461 acagcaagta attttattga agaatatcca gctggctgga gtccgcttta tgtcgatatc
     5521 gcaaaagatc caaatcaaat ctattatcaa aaagaatgcg tccatccagt cactgaaacg
     5581 gagattggcg gaggaatctt attcgatttt gggcgagcag tgaatggctg tgtagaacta
     5641 atatctctgg atgaccatat cgaatctatc actctatgtt atggagagtc cgatgtagaa
     5701 gctatggata tcgattattg ctactataag caaataggaa tgacaagaaa aatgaaagct
     5761 cgtaaacgcg cttttcgcta tttgtttata ccagaggttt ctactggaat ggttcaactt
     5821 caagtttttc atgaattttt atctctttcg aaacaagctg ctttttcaag cgattcttct
     5881 ttactgaatc agatctggtc agtatcagaa gaagccttca agttatgtag tgatttgttt
     5941 tttatcgacg gtatcaaacg agatcgctgg atttggtctg gagatgcgta tcaatcattc
     6001 atgatcaatc aatatctttt ttttgacgag gaaatcaata aacgaacgat attagcatta
     6061 agaggacaaa atgaaatcaa gcagcatatg aatactatcg ttgactattc gatcttgtgg
     6121 ttgattggta tcgaaaacca ctatatgatg acgaaagact atgaattttt acgcatcgtc
     6181 taccctaaga tgcaatcatt aatgagatac ttgatggagc aaacgaatga attagggttt
     6241 atttatggga gagcaaaaga ttggatattt attgattggt ctgaaatgga taaagaagga
     6301 acgcttgctg cagaacaagt cttattactg aaagcctatc attccatgat cacttgcgga
     6361 gatatcttag ctgaaacata tggagagaac gaaaattatc atactcatct ctatcaagaa
     6421 aaatatcaac tattgaaaca aaacctattt gactactttt gggatgaaga acttggcgct
     6481 tttatcgatt gctatgaatc tgggagaaga aatgtgacgc gtcatgcgaa tattttcgct
     6541 gttttattcg attttgctaa caaagaccaa cagcaatcga tcttaaagaa tgtcttattg
     6601 aatgacacta tcacacagat tactactcca tatttcaaat tctatgaaca agatgcttta
     6661 tgcaaactcg gacagacgaa tatcgtctac caaacgattc ttgattattg gggagggatg
     6721 ttatctcatg atgcggtaac tttctgggaa gaatacgacc ctactcaaac aggaaatgaa
     6781 aagtacagta tgtatggaga tccttatgga aaaagcttat gccatgcatg gggagcaagc
     6841 ccaatctatc ttttaggacg ctattttatc ggtttgtatc caacaaagcc aggttatgaa
     6901 gaatttatga tacaacctaa tctagattca tttgaaacac tgaattgtac gctacctatc
     6961 aaagatggaa agatttcgct tgaaaaaaca aaagataaga tcgtgattac ttctgataga
     7021 acaggcggaa cagtgaaaat gaatggtaaa agttatcctc ttctagcaaa acagccactt
     7081 gtcttaacaa gctattcaaa aataaatgca taaaacaaaa aactgcttgc gacatatcag
     7141 aagactttaa atcttcttct gatattgtcc aagcaatttt tattttgaag tttgcttaat
     7201 tctgttcttt agaaacaatg tttgttcttt ttccggcaaa aagaaagcta gataatacga
     7261 caagtaaaaa gaaagcgatg attccaactg tcaaggctat ttgaccgtat tctcctagtt
     7321 tcccaagtac aagtcctggt actagataat cagtatcggc aaatgtagaa gaagcgattt
     7381 taagatctcc taatactgga aggataaata atggcagcca actaatcaat aagctattga
     7441 aagcagaacc taataccgcc cctcttctac cgccagtcga gttgccgaag acaccagaag
     7501 cagctccgca gaagaaatgt cctactaccc caggaatgat aactgtagtt tgcaagccta
     7561 tcatagcaaa catggatagt gttcctacaa agaagctaac aaaaaagcca attaatacag
     7621 cattaggtgc atatggaaaa acgataggaa catctaatgc tggtttagaa tcgggtacga
     7681 gttttttagc aatcccttgg aatgctggaa cgatttctgc caacaccatt cgaacccctt
     7741 gaaggacgat aacaaaccca gctgtgaatg ttccagcttg gatcagtgca aagattacag
     7801 cgttgtctcc attgcttagt tctctttcaa caaatccagg accagctaac aaagcaagtg
     7861 cgacatatac gatcgacatc aagagcatga tcgatacagt agaatctcgc aaaaagctga
     7921 aacttttagg aatctttatg tcctctgtag aacggtcttt gtttccaaaa aactttccga
     7981 tccatccact agaagcataa ccgatatttc cggtatgccc taacgcaact tgattgtttc
     8041 cagtgatgtt cttcataaac ggttgacaaa gagcaggcgt aatagttaat agtgttcctt
     8101 cgaagacccc gcctaataag atcatccaca aatttgcagt aaaccccgcg cttactaaaa
     8161 tgactgctgt tagacaagaa acataaagca ttgcttgacc tgtaagaaaa atgaatttat
     8221 atcttgtgaa tctagcaagt aaaatattca cgatcatccc tactagcatg atcaaagcag
     8281 taggtgtacc gtattgttgc aaagcaatcg ccactactgc ttcattgcta ggaactactc
     8341 cttgagtatg cagagctgtt tggaacatcg aagcaaaagg ggttagtgaa ttactgatta
     8401 aaccagcacc gccgatcagt actaggaatc cgacaaatgt tttgatagtt ccttggatca
     8461 cttctgaaaa tttcttcttc tgaagaacga gaccaagcag tgcaatcaaa gaaacgagga
     8521 tggagggctg actgacgatt gacacaaaca aattcaacaa tttatccatt gatagttcct
     8581 cccttgaatc atttttattc ttttgacaag atcttcgtta ctttttcttt tacttcttcg
     8641 ctgtcaatga tcgattgcaa aggtacgagc ttttcatcag gtatagaagt tagcgtatct
     8701 ttcaaagaat gctcaatgaa gaaataatca gccatatcgc tagatacact tccaacatcc
     8761 gtatgactta cttcaaactc gtccacatct gctccaagat ctgcaagtgc ttgtttgatg
     8821 ttcatctcta ccataaaact tgttcctaat ccactttgac atactgcaaa aattttcata
     8881 tcattcgtct ccttttctta tgatcgctaa tatttcttct tttgttgatg cgtgtaataa
     8941 atcttgaaga ttttgtttat tagaaagtag ctttgttaaa tttgccaaag cccgaagatg
     9001 tgcctcatta tcgatagcag ctaaacagat gaacaaacta atgggatggg ctggattatc
     9061 aagcagatcc acaggttgtt gaagtttcag caaagacatc cctacttcgt ttacgccatc
     9121 ttctggacga gcatgaggta atgcgatgtg atcgccaata tggataaacg caccatactg
     9181 ctccacgcgc tcgatcatag ctttgatata actttcttct attttatttt ctgataatag
     9241 cggctgtgcg gcaaatgtga ttgcttcttg ccagtttttt ggttgatccg ataattggat
     9301 catttcttct gttaataatt cagatagcat aggacgttga tcctccttct tttctaaaag
     9361 tttgttcatt ttacgattta atacttgata gagcttttct tcgtctacgc cttcttttaa
     9421 ttcgatataa ggcagtagcg ccgacaacaa ttcttgggca gaaggcattg aaaatcctgg
     9481 tatcagccaa tctctttgta cttgattgat cagctgattt ttttccaagt cagtcatgat
     9541 tggatgcaga acatagagag actttttctt atcagtaagt gtgagaggta cagtagaaaa
     9601 aataatatcg taagaagttt cgtcaacctc gtgtaagcgg tcagctgaat tagtctctgt
     9661 aaaaaagatc atcgggaata attgctgaag ttcacttttt aagatcaatg aagagctaat
     9721 gccacttgga cagacgatca cggctcttat ttttctattt ctttcttctg cgtccgcttt
     9781 tctgatctct ccgccaaata gtatagtgaa aaaaccaatc tcttcttcag ggatgctctt
     9841 ccctattcgt ctttctaacg gaagtaatgc ttttcttgtc atttcgtaga tggaagcata
     9901 ttgatgctta atttgatcga tcatcacatt aggtaaataa aaagaatagg aaatacgaaa
     9961 ataggcaggg accaagtgat aaaagacatt catcaataat tcacgaaacg aagcaaattc
    10021 tacggccatc aggaattcca tgcgatggat gacttctgaa gaacattcca gcaaaaaatc
    10081 aagtgcaggc tcctggacat ctccttcaac gatcgatgct aagcaaagag agaaaaatat
    10141 aatctccgaa ttttgatgtt cggaatagcc aaggccagtc aatacttgtc gactaatgtc
    10201 agtgactgca agagaagcca acagttcagc atcttttacc agttccatgg atgtttttcc
    10261 aattcgaatg gttgtcaaac tcaaataata agctaattca tccattcgat ttggtgcgaa
    10321 tcgaagatct gatagtgaaa tcgttttatc tagcaaatct ctaactttgg cgtaataatc
    10381 aatggaaagg gttgcggtta aatacgtgag cccaaatctt ccagcaagtg actgcatcaa
    10441 acgagcagtg cgattttttg cttctcttct taatattgct tctgacccta gcagcctaaa
    10501 accagttttc ctagtatacg acagctgaca atcttccact tctaattcat ttcttatctt
    10561 tttgatatct gacaggactg tccctttaga aacctgaaga aaatcttgaa aatgaaaaac
    10621 agaaacagac gttgcagtcg aataaaccat caaataaaga agatctcttc gctcttgttc
    10681 ggtatacaca gcttcaaatc gagtaatatc caaaaatttt tctgcccagt cgattgctcg
    10741 gaatttcttt atacggatac gggtatcatc cacatattcg gcaaaaggag ttaattgttt
    10801 gatcgcctcg cttgtattta cttcgatgat ctctgctgct ttttctagac tgatttctct
    10861 tggtgttttc aggagataac taattttgta gttcacagcg ctgttcacct cctacttata
    10921 ttctagtatg gtagcgcttt taaatcgagg ggtgtttggt acaacttaca gttggatttt
    10981 ttttacgact gtaccgaacg atttctatcg aatggataca gaaaaaaagc aggtgaccaa
    11041 atgtctcctg cttttatgaa ttgatttcgt ccgatcaatg atgatcatgt ttattttttg
    11101 aggtcagttc ttcaaatttt tctttgacat cttcaaccgc tttttcgata tctttttcaa
    11161 agtgattggg ctcattaggc tttgtttcct cttctacaga atttgaacca gataaagctg
    11221 tttctttatt ttctgtttca ccagagtttg tgccagctaa tgattcttct ttgtcttctg
    11281 tttcaccaga attagttcct gctaaagcca cttcttcttc tgttttaggg gccattgaac
    11341 ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat gttggttcct cctagtagtt
    11401 ctatactttt tcatcgtaca ataagacagt ttatgcgtca aaaaacaaac ccgattctta
    11461 caaatatgtc atatttttcc ttctgattct tgaatgcttt tgattccttc ttccaaaatt
    11521 ttcatttgta agattgtttc ttcatctttg atgacttttt ctgcgccatt aactaatgtc
    11581 tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct cggaaattac tttttcttca
    11641 tgataactcc cttgttcatc tatataggtc aatacaccat aatgttcggg caaatcgata
    11701 ccaaagtctt catgatcagg catgtaaaac attttcagat gttcttcttg tcgatctttt
    11761 gtttgtttca cgaacatccc tttttttccg taaacgacga aacttggacg ttctttgatg
    11821 cggaaataac tggattttat cgaaattttc atatttccat agaaaaaatc cagatcgaag
    11881 taatcattca ttcgtccttt ccctaaaagt tgacgtacat catagctgat atcatctggt
    11941 tcgccgaaat aagaaagaac ttgatctact gtatgacaag catgtccata taaatagctg
    12001 ttcgcagtag aaaattcatg cgtattttct ggtacttccg gtctgaaata atcataatgc
    12061 atttctactt ctaaaatatc ccctaatttt ccgctttcga ttactttttg gactgtcaag
    12121 aagtcagaat cgaaccgtct gttttggtaa cattggataa atagaccttt ttcttttgct
    12181 aatgcaaata actcttctgc ttcctttgac gtttctgcaa aaggtttctc tactagtaca
    12241 ttcttaccat gttcgagtgc catttttcca tattcataat gaaattgact aggtgtagtg
    12301 atgacaacta attggatctc aggatcatcc caaagatcag acagctgatc agtatattcg
    12361 atgtcttcga atgttttcca cactggttca gatggggaaa aaattttttt gatattgatt
    12421 ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat ttgtactctt tccattgcca
    12481 agataagcga ttgtaagcat gagtggtgcc tccttttatt gatgttttca gtatatccta
    12541 gaaaagcgca gaaagtattt ataatagcta ttctggtaaa aaagttcaga aaaacacttg
    12601 ttatcattcg gtttggtgaa attaatgttc aaagtaagga taaaaatcaa aaagatagta
    12661 gcggaaccaa gtgtttatca gtaagataga atttggaggt cagatcatgc ttattgaaga
    12721 aaaactaaca aaacaagagt tgtttaccac gacagagaaa cggattgtcg attacatccg
    12781 taggaatatc gaagctgcag tctacatgac gattgaagaa ttagcaaaag ctacgtatac
    12841 ttcccattca gctatcattc gtttatgcaa aaaaatggga ttcagcggat ttaaagaatt
    12901 ccgtttcgag ctggggcggg aagttcatca attgattgct cagttcaatc aaacgattga
    12961 tcctaatttt cctttttcaa gtgaagatag cactcagcaa attgcaaaga aaatggcgga
    13021 tttatcgatc cagtcaataa aaaaagcaca attgcagatt gaacatgaag atttagatca
    13081 aatcggtaaa gtattgaaca aggcacagcg aatctttctt tttgcaaaag gtgattctca
    13141 aatcacggca aggaaattcc agaataaaat ggtcaagttg aataagtttt tgatcatggc
    13201 tgaagaatac agtgattctt cttggaatgc agccaatttg actagcgagg attgcgccat
    13261 ttttattagc tacagcggtc gcatccatca ttatgaacga ataatgacct atttgaagca
    13321 tgtagaggcc cctaccttgc tgattaccgg aaatcagcat tcagaaatgg ccaaacaggc
    13381 tgatatgtgt cttgttatat cgcaggagga atatgatttt gctaaagtag cgactttctc
    13441 ctctcaaatt gccttcgatt acgtattaaa tacgttattc tcagtgatat attctcaaaa
    13501 ttttgaagag aatatccgca acttgaaaaa caaacaagaa ttgatgcaaa aaggccttct
    13561 taaagaatat cggaaaactt aagaaaagat cctcatttag taataagaaa gatagtgttt
    13621 gataggctct ctttgatatc atcataaaaa caggctgaag ttttcttttt caaacctcag
    13681 cctgtttgtt tacggaacga ttcgaataga tatattatag aaatgaggaa tagctaactt
    13741 ctgtttctta ttttgaacga ttgacgaaaa agatatataa gaaggaggaa aatcagtata
    13801 catgcagcta acaatccata caggaaaaat cgtttgttct cggatacttt ctgtacttct
    13861 ttttgaactt cttttgcatg ataaggaacc cggtgccctc tgacaagtaa gcgatgactg
    13921 ttgaccatat aaggtgtaca agttaggagt gtcacgaaat cctgcccttt ttcgattctc
    13981 agatcttttg tatcagtcgg ttctaccgtc ttgatctgat cgacttgata agcgagtgtc
    14041 cgtccattga tttcgatata gaaagaatca cctaaagtca gtttcggtaa gtctgtgaac
    14101 aactttgctt gaggcagtcc tcgatggctg gaaataacgg catgtgtatt ttctcccccc
    14161 gtaggataag acgttccttc caataatgag gcaccttttt ctagcagcac agtattcgtt
    14221 tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg tcaaaatacc gatcgtatgt
    14281 ttctcaaaat aggttctatc ctttttcgca ggttcttttt tcttagtaaa gggatctgca
    14341 cctggatttc caccttcttt tgctaattgc tggtttttct ttgtcatgtt ttcttggatt
    14401 ttagccatga cttcttcatt ttctttgacc gcttgttgct gataatgaga gatgatctgc
    14461 tgatccagat aattattcag cgcgtcgcta acaaatggat aagctagtat ccctattcct
    14521 gagagaagca agaaaagcat cagcagatct agaagtttcc ttttctttgg tttcatttgt
    14581 gttctccttg tgatgactgt ttgataataa aagaaaaagg agcatttgcg taatccgctg
    14641 ctcctttttc caattcttgc aaaaagacta aatctgactg tgcttgcgtc tagcaaagta
    14701 cagtccagca atcagtagaa ggactactcc tgcaccgata tacacataga ttcccttacc
    14761 gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt ttttcaggag aaatcaactg
    14821 tcctgctgtt acataagatt gttcatcgat agtaaatgct acacggtttg ttaatggaac
    14881 atatttttct ggcgctttcg tttcttccag atagtacgtg ccatatttta gacctgtcac
    14941 atcgattaaa ccgttacttg tggttgtaaa aaccgttgct gattctttcg ccgataccca
    15001 gctgacggct tttgtggatg ggtcgatcga taaatatttc gctgtgtcac tatcttgatc
    15061 acgaacgacg aattctgctc cagcaagtgt ttggtctgat gtaacgtcac catctacttt
    15121 aacaaatcgt ttgcccccag taacgacatc gactgacggt ggtgtttgat catttgtatg
    15181 cccgttatcg acatttgctt ggttagaaaa tcctttggtt ggatctgctt tttcgttcaa
    15241 atgcatatag taaacgaatt tcaatgtacc gccaggagtt aatgaaggaa tataattcgg
    15301 atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca gaataattta ctgggtcgat
    15361 ttctttattt ccatcatata aggcataagc atacgttcct gaagaatcat tatcaaatgt
    15421 taaagcagcg tcatgagtat cgataagttt gaatgttgtg tacttgtttt gcgttccttc
    15481 tttatcagcg atacctaatg ggatattgac agaaatctca tattgaattt tttcaccgat
    15541 tgcgacatct tttccattca attgaggtgt tgttttatca actttagacg tatcattttt
    15601 gatggtcttt tctactggtg tttggctatt tgccaggatc tcaaaaggcg tgattgtttg
    15661 cttttcaatc atttccgcat tatcaggagc ttttacttct tctaaattat attttccaac
    15721 ttctaaatga ttaacgatca actggccttt ttcaatagat gcctcggcaa agtcattgtt
    15781 gccgatgtca taagaatgac cagtaatgaa atgtttggct ttggtttgat cagttgtcca
    15841 agtgtacaat ccatctgtga cactttggat gtattttttg acgcttggtg ttccttcttc
    15901 tttagaaata ataaattctg ctccatttag tgcttcgttt tcggcagtac cgatttttgt
    15961 aactttcaac gttccatcat taccgactgt atttttaggg tagagatgga tagtatctag
    16021 ttcttctgtc ccgtatttat aagagccatc tgcttgtttg atcatctcat atacagggaa
    16081 agctaaaacc atgtttgcgg cagctgacac tccgtctttt ggttcttctt tgatcgtgta
    16141 gactgcatct ttcccatttt gttttttagg taaagataaa gtgacatttc catcagcatc
    16201 tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac tggactgctt gttttgctgc
    16261 atccacggac gctcctttat ctcgttgcgc ataaaattct tgagtgacgt tataaactga
    16321 aaatgaaata tcggctaatc cttggtattg atcgaattcg ctcatttctt tcccgctgtt
    16381 ttggattaaa ggatcgggta aatcagtcat tttcttttta tgaaggatga cttgagctgc
    16441 tgtctcctct gcaaacactt tttttgcgcc gaagctgttt gtgagtaatg gtaaaataag
    16501 gaaaaggact cctaacataa cgtttatttt tttatgattt ttcatttttt cactcctgtt
    16561 ctttttttga taaacaacca gattgcaaag cttgcgaata ccataccaac aacgcctaaa
    16621 agtgtatttt tcgtttcgtt tgtttttgga agaaatggac ggctattttc ttttttattg
    16681 actactttca atatactgtc cgcctcttta tcctcagaaa gaaaagttga aatggtaaac
    16741 ggaatttctg tttcacttaa aatataacca gaaggtgctt cgatctcttt taatcgataa
    16801 cttcctgttt tcaacccgga aattgaaaaa gcgccatttt tatcggaaat caggtgaagc
    16861 gcatctgatt cgttctttgt ccacttgtat ccgttggctg tttgctggag gtaatttcct
    16921 gctttgtttt taataaggaa tactgcgcca gctaatcgtt tatctggttt atttgtctcg
    16981 attttgacaa attgcttact ccctgtatat acagagactt tctttgtact gacaccaaaa
    17041 ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg cctgtgctgt actgcttagc
    17101 tgcatctgat aagaaatggt cagttttttt ccagcatatt tttccaacga agggatcgaa
    17161 aataaaacat caaatccgtg aggtttcttg tgtatgacaa agccttctgt cagcttttct
    17221 ccattcgatg aaatcgttaa actattaggt aaaaacgtta atgcttcatc cgcactatct
    17281 gacaattcga acttttgata gtcaaggata tttacaggaa ttgtcgtatg caaggaataa
    17341 gtgatttttt caccaatcgt aaaatcattt ctaggctctt ctaatacctt ctcaaaaggt
    17401 ggatcagggt agtcgttttc ttcattttta ggatacaaat ggatagttga aagtttttga
    17461 ttgttttgtc catgtacagg aagaacaact accatattct ctgccttttc tttgacgact
    17521 tctggtactt tggattcaat gaaaagatac gctttatctc ttttttcttg atctttagcg
    17581 gccagagaaa aagaagcgat accatcttcg ttgttaagag ttgtagttgt tgcttctgcg
    17641 gtaaacatac cggaagacgc accgtttttt gcgatctctg cttgtgcttc ttctaccgtt
    17701 ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat cataaacttg gaatgtgaca
    17761 ccatttaatc ctcgatacgt ttgtaataaa gctttttctt gtccgtcatt tggatgattt
    17821 ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag taatctgaac catttctgtt
    17881 tctgtttctg ttgctacctg agtaaaagct ggtttaaata gtaacaagaa gagacacata
    17941 ctaagccaac caagtttttt catatttagc tgccccctct ttcttttttc aaaaaaagat
    18001 accaaataga aaacgcacaa ccaatcatcc ctactagata gattcctaaa cggccaattc
    18061 ctcccgtttc aggtaatggt acttttgcct gattcgtgat gtctaaagaa atctggttgt
    18121 ttttggctcc tggtgacaga acagattcat gatcttgtcc atccacttga attgacccat
    18181 cttcgtgtat aactatagta actggctctt ttagaccttg gtatccttct ggtgtaaaag
    18241 tttcggtcag cgtataagtt ccaggtttta aattttcgaa tagaaaggta tctgtttctt
    18301 gtccatcttt tggcgattct aagtccattt ctggtccctg cagtctgaat tttgctcctt
    18361 ttaatgtctg tccgttgtct tcttttttgt gtaccgttag atcaaacgct tttagtcgat
    18421 tttgatgagt gagcgtccat acattatttg tcatctcggt attttttccc gtgtaaatga
    18481 tttttccata tttatcgatt tggaattcat aatttcccgg cgaagtatca tatccggcag
    18541 gaccagctgt ttcaacgact cgatatttac caggtgtgag tgaatcaaaa ctggcaagac
    18601 ctttttcatt cgttgtttgg ctgtcaattg gctgataagt accatttgct tcttttttct
    18661 gtagggaaaa tgctgctcct ttaagattta tctctttgtc cgtcccttgc atcgcatact
    18721 tacgtactgc tacaggaact tcaacaaaag gattttcaat agtcaactgg atcgtatcat
    18781 cggaagtagt gactgttttt ccatcaatgg ttaccgtacc atcagaagcg atcttgattt
    18841 cccaagtagt ctttttgctt aatccatgcc cttctggagc ttttgtttct gtcagcgtat
    18901 aggtcatttc tttttgcaat ttgacatttt ctggaagaga ataggttccg tcgccatgat
    18961 ctgttagtaa agtatcaata gaatctcctg ttaatttgaa aacagcgcca ataagatcct
    19021 tttcacctgt agaggaattt ttcgttattt tcaagtttaa cggtgtgaat tgtttagtat
    19081 ttttccatgt cattgcatct atttgttgag aatcgtatcc tggtacaggt aattctttga
    19141 ttgtttggta actgaatgct tgaccttgat tattgtattg aggtagtgat aatatctctt
    19201 gataactttc tccgctattt gcagataatt tgtcaatgtc tgcacgttcc cacgtatttg
    19261 ttgtatcttt agctggttta atgattcgaa tatatccgtt tttccaagct gcagcatttg
    19321 tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg acgatcagct agattgttgt
    19381 caaattcttc ccataacttt ttgatgtgaa gactgactcc gggagcttta gcagatggta
    19441 taccgaattc agctaattca ttggtatcaa tgtttggctg gaaagttgtc ctgccgttca
    19501 tttgatacca gaaatttgga tggaagtcct cattttctgt ttggattctc acttggtaat
    19561 ggatttggat ttcttggtct tttcctaaat agatctgatt gctcttgatg gtatttcctt
    19621 ctatggaaat agatggagtg accgttgtag gacttgtccc cacactcttg actgaaagtg
    19681 taccaggctg ataaatgaat ggttctgcga ttggatcatt tatttgtcca ttgcttacag
    19741 ttgctgagat ctgtacagct tttttggcta ggtattcaga gatatctgtt gcatgatcag
    19801 ctgattcata gtaaagatcg cctttttcat ctgatgaaac catttgtcgc atacgagact
    19861 ctacttctgc ttttgagaga ccagctgccg gatcgctttg aagttggatg ccaagaccat
    19921 gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc gatggttgcg ataaacgtac
    19981 tgtcgattct tctggataga ttgttttggt caggtgcatc atagattctt gagattagag
    20041 aagtatttcc cggccgatct tgcgtattag aaaactgagt tccgtaataa ttgctgcttg
    20101 attgtgcgtg tacccgctgt actttatagg aaaatgttgg tacaccatcc gtcagcaaaa
    20161 cgatcacttt tttatgtcca tttggaacgg atagcatgct tcctgcatct cttagtgctt
    20221 tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat ggattttact tgatttttca
    20281 ctgaatcaaa tgaacccatc tgtactgcac cgttactgta gctatatcct tcgcttgagt
    20341 agccgacata tcccatattg attttgtctg tgataccgct atctgctaaa gtatcgacaa
    20401 aacgatcgac accaatcttt acttcaccga tccgattatt gtcgttcata cttcctgacc
    20461 agtctacgac caatacgaga tcaagaggcg tgatatcttt ttgtacattt ccacgagcat
    20521 tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt tgcatacttt cgtatcgcat
    20581 agtctgcttg attaccggtt cctccatatt cgatatacga atgggtccga tcattaggat
    20641 ctccattcca agaatttata ccatcccatt gatttgttcc gtttttattg ccttgatgat
    20701 caagaacatt tgtatttcct gtaggttgcc aactggcttt aggataagtg ccctgatcat
    20761 ctgttgtata ttctgtagga atcgtttgat tagatggcac actcacagct ggcaaggata
    20821 atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc ttttgatgat tcttcttcta
    20881 tttcgtctgg ctttggtact gacacatctg tatctttctt attttctgtc tcatcaataa
    20941 tgtcatattc tttattatcg acgatcagct tgtttgcctg gctgtggtaa agtgctggat
    21001 cgattggaaa actcagatcg atagtttgtt tagtgtttga tgagatttcc aacgtaattt
    21061 tcccgctatt tacctgataa gcatagccat ctccttcttt tctttccaca tcacttaagt
    21121 aattttcgac tagaccaatt tctttttcta tcttttcagc actggtgtta ttcgcattta
    21181 gcttgatgtt cagtgctttt tttccagact gatccgaaat gacttcagct gaaattgttt
    21241 ctaaaactgt tggatcatct gtagtgtctg ctattgcaga taatggcaca aagtttgata
    21301 aactcaatat tatcagcatg aaaataactt tcagtttctt ccctgtggtc gtcatttttc
    21361 ccctcctttt tctttttttc tgttttgacg tgaatagtga aaattattaa aataaacagg
    21421 cgttacataa ttttatttat tttaatatga taattattat acgaagggcg ctaaagggaa
    21481 tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc gattaatgag
    21541 cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg ttggaacgaa
    21601 actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag tggatgacta
    21661 aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg tatatcgaag
    21721 agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa ttggggtatc
    21781 gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg tcgcaaagtt
    21841 ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg attgagtatt
    21901 attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa caatgcaatc
    21961 aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag ttaactagcc
    22021 ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt tactacggaa
    22081 gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg gtcaagaata
    22141 attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc tttattatgt
    22201 attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg aaagctggag
    22261 actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg aaaaatctct
    22321 taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc aatacgattt
    22381 tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga aaaaatatgt
    22441 cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat gttgttcaat
    22501 tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact catttgacga
    22561 tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta tatgctggat
    22621 ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt tattatttat
    22681 ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat acttctgata
    22741 aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt gaagaatata
    22801 tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt actgaagatc
    22861 agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa cccattttga
    22921 atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat gaagaagaac
    22981 tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac caccctgata
    23041 aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc agcaatccaa
    23101 cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt tgcaatgaga
    23161 tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac tgcttcttcc
    23221 gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc gttcattgga
    23281 gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg ggttaacaag
    23341 tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc taaatatttt
    23401 tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat atcttgatgg
    23461 actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc ttgaaaatat
    23521 ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac tttgacttgg
    23581 ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg tttctttgcg
    23641 ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc tctgattata
    23701 cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt cttctgtcaa
    23761 gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga catcatgagt
    23821 aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt tgacgacttc
    23881 atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca agcttggctc
    23941 attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa ggtctttagg
    24001 ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga tttttgttga
    24061 atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta ccgttaaaaa
    24121 aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta aatctgctcg
    24181 ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc cggaagtcgg
    24241 tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag aaggaccaat
    24301 gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag ctgagacctt
    24361 tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt ttttcatttt
    24421 tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat cgaaaacaat
    24481 ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg tgaccaaaaa
    24541 acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa caaaagagca
    24601 agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt agcgatgaag
    24661 aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg aagtgtcatc
    24721 acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta tatcatcgca
    24781 tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt cccaatagtt
    24841 aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc ctttattacg
    24901 atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata cttcaaagca
    24961 gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt ttcaggaaag
    25021 ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc tccaatttta
    25081 tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt aaattcatgc
    25141 cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac tgtaacattt
    25201 tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt ctctcctcct
    25261 tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg atttgcatcc
    25321 tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa ctcttctgaa
    25381 agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa cataataccg
    25441 acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag aaacatttgt
    25501 ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata gtgtaactct
    25561 aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc taagacatct
    25621 ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt atgctttcca
    25681 tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat tagtagtaga
    25741 agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg gcgagataac
    25801 acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt atgatcttgc
    25861 aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa aaatacggaa
    25921 agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg aagataagat
    25981 tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc gagaagaact
    26041 gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg aaaatgttct
    26101 gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata aagaacaagt
    26161 tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa ataagggaat
    26221 cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg atacgacgat
    26281 tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta ctggattgga
    26341 taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt gatgcaatga
    26401 atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta gcagcatttg
    26461 cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa tctgcataca
    26521 gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta caattagaga
    26581 tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa caaaacagaa
    26641 caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact caagtgatct
    26701 tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt ttcaaccaaa
    26761 tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt gaattaaatg
    26821 aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg gagacgaatc
    26881 aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag aattcattag
    26941 cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc tctattggta
    27001 tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg agaagacaaa
    27061 aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt attcaaaata
    27121 gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc gaaagcatcg
    27181 ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg ctgacaatcg
    27241 ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca caagaatata
    27301 tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa gagtttattc
    27361 tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat caagttttgg
    27421 ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc attttggatt
    27481 catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct tctatcagtg
    27541 aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct cgggcgaaag
    27601 agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa cagcatcatg
    27661 gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc caattgccgt
    27721 tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat cttgtcacag
    27781 cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa taagccgaaa
    27841 tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg attttttatt
    27901 gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc ggctaacatc
    27961 ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt cttcacgaat
    28021 aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa tcgtgtcatc
    28081 ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc gttcgatgac
    28141 gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca catgtttgtg
    28201 gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt cttcttttag
    28261 ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct tgcttgtacc
    28321 cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca tattcaatgg
    28381 atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg ttccagtgcc
    28441 agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg tatccattgt
    28501 atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt ttccatcaga
    28561 aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct cagaatcata
    28621 ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt cttcaccatc
    28681 catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc ctagtctaga
    28741 tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt cgttacgcaa
    28801 ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga ccgttaaatt
    28861 tttctcttcc atgtaaattc ctcctagcaa atagatttat gattggtata ttgttactta
    28921 taaatttatt ctataggaga cgattgtttt ctacaaatat ttagattgga atttgattaa
    28981 aacaatttaa ttgaaatttg aaaacattta tgttaatgaa agtcatattc taataaaatg
    29041 tcatttgttt ctatatgaat ttttatgaaa aatttcatat aatttccact cgttgtgatg
    29101 gtttaattta tccaatccat caataagcaa acaacttata taaaaaagga ttaattctca
    29161 atataattgc tcctaccata gaataaatct tcatgtaaag taatgaagta ccaactattt
    29221 ctaatacttc tttaagaatt atcccaacaa tatcgttaga caaaccatta ttgaataaat
    29281 ggagagccaa tcgattctga aaaaatgttt tctaattttt cggaaataaa agaaagagcc
    29341 tcctgccatt agaacgaacc aaatccattt cattgctcat gaagtaacca catagctaaa
    29401 aagaggataa atcaattaaa agatcaaaag aatgactgca ctaataaaat aactaaaaat
    29461 gtatcttctt acttcatatc ttttagctag tacttagtta aaaatttgct tcctaagatc
    29521 aagacaaact gtctatcttc atttgatgaa atgcggtaaa atatagaaaa tgaacactat
    29581 tggagggaat ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa gttaaagcaa
    29641 cgtacacact tgctcccttc cgtctttcaa cagtttttag aagaaaatcg ttcttccctt
    29701 tcattaggca gtcgctatga atacgcaaaa gattttcatc tgtttactga ttatttgaga
    29761 aaagtatttt ctgaagaagt ggatgatcag ctgattatcc atttagaaaa acaagattat
    29821 cagaatttct tagctaaaat ctatcgacat actcgttcgt tccaaactac agcgaaccaa
    29881 gaaacggagc aattgtttga aaatagctat tacgggattt caaggaagca atattcatta
    29941 aatcattttt tacgattttt gtatcaaaaa ggactgattg aagaagagat ttctcttttg
    30001 ggtacaagtt taccggaaac aaccaaagca gctccgcgat atatcgatgc agaacttttc
    30061 aaagattttg agcatttgtt tatcccgatt gaaggctttc aaacaagcag agaagctagc
    30121 tttgaagaaa agaatagacc gaggaacctt gcaattactg ccattcttct gtattgcgga
    30181 ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg tatctttgac caagcagatt
    30241 cttaatctga atagaaaaga aaacagactg aataaagtac caataccaaa agaagcgatg
    30301 ccatttctgg agaactattt taagttaaca gcacaaaata cggacgaaaa ttcttttgtt
    30361 tttcgttcct cacatgatca gcaaatttct cctcggacta ttcgccaaat tttgagcaaa
    30421 attacagtat ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg ttattatgca
    30481 gaactatatt tagatgatgc tgacgcggta aattatttat gtggtaatcg atacctctca
    30541 tcccattctt ttcaagatat ctgggaaaag atggttgatt tttcttatca cgaattagcg
    30601 gcattcgttc gggtttcttc catataaaac aaatgtatga tctttttttc acaataaact
    30661 atgttattat ataaaggatt atgttacgat tatcttgaat ttgtatatta cttaacaaaa
    30721 gataatggag tgtgaatgat ggagaccata acgtattccc ctttgaactt atttacgaat
    30781 tttcaagaag ctgcagctaa cttttccgag accccgatta tctttgatga gccactttca
    30841 gctttcccag aattaggact tgaaacaact tacaaaaaat gctgcgaaag tgtgttgaaa
    30901 cgggcttatc aattggctca tcttggagtg aaatccggag acaaagttat tatttataag
    30961 agttccgctt ttgacacata ccttctagct gtttctgtgt cttaccttgg tgcagtgcct
    31021 gtcatgactt cttatcatct accaacaaca acaatggaag tatttatcga tcgtttagaa
    31081 gaccctttca ttttatttga tgatgagaca aaaaagcgag tgagagaaat ttcaaatgga
    31141 acaaaatcaa aacagattc
//
LOCUS       DAFFFQ010000023        30945 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000023
VERSION     DAFFFQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.023"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            160..1101
                     /locus_tag="LQB69_001186"
     CDS             160..1101
                     /locus_tag="LQB69_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL3525362.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1170..1901
                     /locus_tag="LQB69_001187"
     CDS             1170..1901
                     /locus_tag="LQB69_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL3525363.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2045..4225
                     /locus_tag="LQB69_001188"
     CDS             2045..4225
                     /locus_tag="LQB69_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL3525364.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAFLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4245..4727
                     /locus_tag="LQB69_001189"
     CDS             4245..4727
                     /locus_tag="LQB69_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL3525365.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4743..5504
                     /locus_tag="LQB69_001190"
     CDS             4743..5504
                     /locus_tag="LQB69_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL3525366.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5635..6969
                     /locus_tag="LQB69_001191"
     CDS             5635..6969
                     /locus_tag="LQB69_001191"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL3525367.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7276..7761
                     /locus_tag="LQB69_001192"
     CDS             7276..7761
                     /locus_tag="LQB69_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL3525368.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7754..8437
                     /locus_tag="LQB69_001193"
     CDS             7754..8437
                     /locus_tag="LQB69_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL3525369.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8563..11208
                     /gene="polA"
                     /locus_tag="LQB69_001194"
     CDS             8563..11208
                     /gene="polA"
                     /locus_tag="LQB69_001194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL3525370.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11253..12089
                     /gene="mutM"
                     /locus_tag="LQB69_001195"
     CDS             11253..12089
                     /gene="mutM"
                     /locus_tag="LQB69_001195"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL3525371.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12053..12682
                     /locus_tag="LQB69_001196"
     CDS             12053..12682
                     /locus_tag="LQB69_001196"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL3525372.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12734..13066
                     /locus_tag="LQB69_001197"
     CDS             12734..13066
                     /locus_tag="LQB69_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL3525373.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13378..13869
                     /gene="nrdR"
                     /locus_tag="LQB69_001198"
     CDS             13378..13869
                     /gene="nrdR"
                     /locus_tag="LQB69_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL3525374.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13892..15265
                     /locus_tag="LQB69_001199"
     CDS             13892..15265
                     /locus_tag="LQB69_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL3525375.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15265..16191
                     /gene="dnaI"
                     /locus_tag="LQB69_001200"
     CDS             15265..16191
                     /gene="dnaI"
                     /locus_tag="LQB69_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL3525376.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16320..16760
                     /locus_tag="LQB69_001201"
     CDS             16320..16760
                     /locus_tag="LQB69_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL3525377.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16904..17605
                     /locus_tag="LQB69_001202"
     CDS             16904..17605
                     /locus_tag="LQB69_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL3525378.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17602..18723
                     /locus_tag="LQB69_001203"
     CDS             17602..18723
                     /locus_tag="LQB69_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL3525379.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18738..19970
                     /gene="pepT"
                     /locus_tag="LQB69_001204"
     CDS             18738..19970
                     /gene="pepT"
                     /locus_tag="LQB69_001204"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL3525380.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20102..21010
                     /locus_tag="LQB69_001205"
     CDS             20102..21010
                     /locus_tag="LQB69_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL3525381.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21045..21317
                     /locus_tag="LQB69_001206"
     CDS             21045..21317
                     /locus_tag="LQB69_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL3525382.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21328..22374
                     /locus_tag="LQB69_001207"
     CDS             21328..22374
                     /locus_tag="LQB69_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL3525383.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22625..25234
                     /gene="clpB"
                     /locus_tag="LQB69_001208"
     CDS             22625..25234
                     /gene="clpB"
                     /locus_tag="LQB69_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL3525384.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25385..26071)
                     /locus_tag="LQB69_001209"
     CDS             complement(25385..26071)
                     /locus_tag="LQB69_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525385.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26049..26543)
                     /locus_tag="LQB69_001210"
     CDS             complement(26049..26543)
                     /locus_tag="LQB69_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL3525386.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26563..27783)
                     /locus_tag="LQB69_001211"
     CDS             complement(26563..27783)
                     /locus_tag="LQB69_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525387.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            27930..29765
                     /gene="lepA"
                     /locus_tag="LQB69_001212"
     CDS             27930..29765
                     /gene="lepA"
                     /locus_tag="LQB69_001212"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL3525388.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(29859..>30945)
                     /locus_tag="LQB69_001213"
     CDS             complement(29859..>30945)
                     /locus_tag="LQB69_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3525389.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10302 a         5542 c         6629 g         8472 t
ORIGIN      
        1 ttctgtgcga aacaggaaag cgaagtcttt ttaattcaaa taataatatt atgtaaacaa
       61 aaaactttcc atgcgtaatt atacacaagc aaataaatcg tcaaatgcga acgccttctt
      121 tatactgttt aggaacgaaa agaaggaagg aagaaataca tggattatca agtactgctt
      181 tattacaatt atacaaaaat cgaagaacca gaggaatttg ccaaaaatca tttagccttt
      241 tgcaaatctt tggatcttaa aggtcgtatt ctagtagctg aagaaggaat aaatggtact
      301 gtgtctggaa ccgttgaaaa cacgactgcc tatatgaaag caatgaaagc tgatcctcga
      361 tttgctgata cgatgttcaa gatcgatcca agcgaaggac atgcctttaa gaagatgttt
      421 gtaagatacc gtccagagct tgtaagttta aagctagaag aggacattga tccaaaccaa
      481 gtgacaggtg cctatctttc accgcaagaa tttaaagaag ctattttaga tgaacagaca
      541 gtggtgattg atgcacggaa tgattatgaa tatgacttgg gacattttcg aggggctgta
      601 cgcccagata tacgcagttt tcgagagctt ccagactgga tccgcgagca caaagaagaa
      661 tttatggaaa aacgggtagt gacctactgt actggtggca ttcgttgcga gaagttttct
      721 ggttggcttg tccgtgaagg attcaaagat gtcggacagc tgcatggcgg tattgctact
      781 tatggtaagg atcctgaagt ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat
      841 gaacggatcg ctgtcgatat caaccaagta gataaacaaa tcgtcggtcg cgactggttt
      901 gacggaacac catgtgaacg atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt
      961 cttgcttcca ttgaaaatga agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa
     1021 catccgcgca atcgctatgt tcaagaccat ggaatctctc cagaagaagc agaactgcgg
     1081 tttacagctt taattaaata gaaaacaata gttttgattg tcgaccattt ccaatatgcg
     1141 ctatactagc gttgaggatg gtgatccaca tgaaatcatt gatttatgct attggggatg
     1201 tccatggcaa acatgagctt tttgaaaaac ttattcacta ttatgatcca aagatccatc
     1261 agcttgtttt attaggggat ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat
     1321 taggaaagca attagtcgaa gaaacaggag ccatttacct ccggggaaac catgaagaat
     1381 attttttgca atttctgcaa tcaccggagg actggtatgc gccttatatg agaaatggtg
     1441 gaaaagaaac gtttgaaagc ctgattcacc caggggcatc ggaggaatac tccccaactg
     1501 aaatctcgat gatgatccaa tcgagatatt cagaactgat taaattttta gtccagcgcc
     1561 ccttgtatta tgaatgggaa aattatcttt ttgtccatgc cggtgtggat ttgacaaaga
     1621 aagactggcg taagacaagt ctaagagact ttatttggat aagggaacca tttcatgaag
     1681 ggaaaaacaa tacaggtaag acaattgtat ttggacatac gattacacca atgctccatg
     1741 gagatatgca gacaacagac ctctggattc aggatcataa aatcggaatg gacggtgggg
     1801 ctgtattcgg agggtcattg catggggttg tttttgacaa ggaaggtatt cttcaagata
     1861 ttgaatatca gaacaacgaa ggaccttggc atggaatctg actaatggaa agaatgaagg
     1921 agctgtgact ttgttgcggc tctttttatt tttatagaga atattgtttc tttcagtagt
     1981 ttaacccagg ataaaaagta tgatagaata aagaagcaat ttttagcaga aaagaggcat
     2041 tttaatgacg gaaaatttga accagacgat cattcagctt gtacaaaaag aactttcaga
     2101 ctatcgtcct aagcagttga ctactgtact taatttgtta aatgaaggaa acactgttcc
     2161 ttttatcgcg cgttaccgaa aagaaatgac tggaagtctt gatgaagtcc agattcgtga
     2221 aatcgaagag cggtatgcct atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg
     2281 gctaatcgat gaacaaggga aactgactcc cgagttagaa gcagaaatca cccaagccgt
     2341 gaaaatgcaa caagtggaag atttgtatcg tccatacaaa caaaaacgac ggacaaaagc
     2401 aacgattgct aaagaaaaag gacttgaacc actcgcgaag tggctgatgc agcttacaga
     2461 tggagaggtg caatctgaag cagaaaaata tattgacaaa gaaaaagaag tctcttctgc
     2521 cgaagaagcg ttgcacggtg ctcatgagat tattgctgaa caagtcagcg ataatgcaaa
     2581 atttcggact tggattcggt cttatacgta taacaaaggt atgtatgtca gcaatgtcaa
     2641 ggatgagcaa gcagatgaaa aaggcgtata tgaaatgtat tatgactttg cagaacccgt
     2701 ccataaaatg gtttctcatc gaatattagc tactaatcgt ggagaaaaag aagatattct
     2761 gaaagtattc tttcaagtag atgaagcagc aattttagct ttcttggatc gccaaatagt
     2821 caaaaatcct gcctctcctt cttcttcatt cgtcagagaa gcctatcagg atagttataa
     2881 acgctttatt cagccagcaa tcgagcgaga actgcgcaat gaattgactg aaaaggcaga
     2941 tgagcaagca atcgctattt tcggcgaaaa cctccgtaat cttttgttgc aaccgccact
     3001 aaaaggtaaa gttgtcctgg gatttgatcc ggcttatcgt actggatgta aactagcagt
     3061 cgtagatgcg acagggaaag ttttagcaat cgaagttatt tatcctcata aacctgcagc
     3121 gcaagcaaaa agagaagctg ctggaccagc ttttatccaa ttaattaata aatatcaagt
     3181 agatatggta gctatcggaa atggtacagc cagtcgggaa tctgaacttt ttgtggctga
     3241 gcagttgaaa tccgccgacc ataaaaccta ttacgctatc gtcaacgaag caggcgcttc
     3301 tgtttactcc gctagcgaaa ttgcacgaaa agaatttccg catctacaag ttgaagaaag
     3361 aagtgctgtt tctattgccc gtcgcctgca agatccttta gctgaattag tcaaaatcga
     3421 tcctaaagct gtcggagtag gccagtacca acatgacgtt tctcaaaaac gtttggctga
     3481 acaattggat tttgttgtgg aaacagcggt taaccaagta ggtgtggatg tgaatacagc
     3541 aagcccgcag ctcttgcagc atatatcagg attgaataaa acaactgccc aaaacattgt
     3601 catttatcgt gaagaaaacg gagaattcac cgcacgaact caattgaaaa aagtgccacg
     3661 cttaggacca aaagcatatg aacaagcaat cggattcttg cgagtccctg gtgggaaaaa
     3721 cattttggac aacacaggta tccatcccga aagttattct attgcaaaag aaatattaac
     3781 ttcagtccaa ctttcagaaa aagagcttgg tacagaagaa gcagtagaaa aattgacacg
     3841 attatccgta gagaaactgg ctgaaagcct atcagtcgga gaagaaacgt tagccgatat
     3901 tttagctggg ctgacacaac ctggacgaga tatgcgtgat gagatgccag cgcccttatt
     3961 acgtacagat gtattgagta tggaagactt gaaaccaggg atggaattga ctggaacggt
     4021 ccgtaatgtc attgacttcg gcgcatttgt tgatatcggt gtgaagcaag acggacttgt
     4081 ccatatatcg aaactcagca aaaaattcgt caagcatcct acagatgtcg tgtcagtagg
     4141 agatatcgta acagtttgga tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac
     4201 gatgctttcc ccttatgaag agtaacaatg aggagtaaca aaaattgaca caagaagaac
     4261 tgcagcagtt agtagaagag atatcattaa aaagcttcca gcttcctttc aagcaccaag
     4321 caatgttcaa tccacgctta cgtacaactg gcgggcgcta tcatttaaac gatcatcatt
     4381 tagattttaa tgcgaagcta tttgaagagt cagacgaaga gacgattgca ggaatcatca
     4441 aacatgaact ttgccattat catcttcatc tagctggaag agggtatcag cataaagata
     4501 tggatttcaa gtatcttttg aaaaaaacag gagggttgcg ctatgcacca gccttgacga
     4561 agaaacagat aaagatggag tattatcgat gccaagattg ttctgccacg atctgccgca
     4621 agagaagaat cgatgtacgc aaatatcgtt gtggaagatg tcatggagaa atcaaatgga
     4681 ttgagacaaa aagagaagac tttaatgaag cacaaaaaat cagatgaaag gatagaaggc
     4741 ctttggaaaa aagtgagcag aggaatctaa aagactggaa tcagctatta aattttttag
     4801 ccgcacctga agagggaaat tttgaggaac tgacggcacc tacacttatt ttagctggaa
     4861 actgtcttcc tatcttagct gataaagcag cacaaatgta tttaaatggc caagtagatc
     4921 aattgttttt ggtcggaggg gtggggcatg cgactagaat cctttatgag aattttgaaa
     4981 agcaaggatt tcattttgaa gatggcatga gtgaaagtga aatctgtcgt cagtatctaa
     5041 aagaagtcta tgatttgccc gataaggctt ttctgataga atcgaagtct acaaattctg
     5101 gagagaacgc cactttttca ctggagattc ttcattcctt aggagctgta ccagaaaaaa
     5161 tcttattgat gaatgatccg acactgcaac gcaggactcg agcaactttt gagaaagtat
     5221 ggcaaaatga acaaactatt ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact
     5281 tttctgaaga gatcatcttt acagcaaaag aactgaatca tcaatggcct aaggaatatt
     5341 tccatgcgct agtattagga gaaatggaac gtctccatga cgatgagaac ggctatggtc
     5401 cgaaaggaaa agattttatt cctcacattg atatatcaga ggaagtttgg tcttcttaca
     5461 ccagaattaa agagtcaata aaaacggatt tttctcgtac ttgaccattt tagcaagcga
     5521 atcctccttt ccaaataaat ttggttgaaa ttttcaatcg tttataagat aatgaggagc
     5581 gtgttgaatg taatagctaa aagaatggac gctagataag agaagaggat tcaaatggaa
     5641 aatcaaaata aattgtatca ttttgttggt atcaaaggtt caggaatgag ctccttggct
     5701 cttgttttac atgaacaagg tctaaatgtc caaggatcag acattgaaaa atatttcttt
     5761 acacaaagag atttagaaaa agcaaatatc acgattctgc catttaacgc agataatgta
     5821 aaaccaggga tgacgatcat tgcaggaaat gcatttcctg attcacatga agaaatccag
     5881 cgagcaaaag aattaggttt ggaagtgatt cgctatcacg actttattgg tcattttatc
     5941 caaaattata cgagtatcgc tgtaacaggc tctcacggaa aaacaagtac gactggactg
     6001 ctctctcatg tattgagtgg tgtccgtcca acaagttatc taattggaga tggaacagga
     6061 catggtgatc cgcaagcgga attcttttca tttgaggcct gtgaatatcg ccgtcatttc
     6121 cttgcctatt caccagacta tgcaatcatg accaatatcg attttgatca tccggattat
     6181 tatacaagta tcgacgatgt atttacagct ttccaaacaa tggctggaca agtgaaaaaa
     6241 gctatttttg cgtatggtga cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt
     6301 tattattatg gtgtcacaga aaacgatgat atccaagcac gaaacatcga acgcacaact
     6361 tctggttctg cttttgatgt ctatcatggg gatgaatttg ttgggcactt cactgtacca
     6421 gcattcggta aacacaatat cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa
     6481 aaactggatc tgaaagaagt tgcagaagaa atgctgactt tcccaggagt aaaacgtcga
     6541 ttcagtgaaa agatcgtagc agacatgacc gtagtcgatg attatgcgca tcaccctgca
     6601 gagatcaaag caacgattga tggcgcacgc caaaaatatc cagataaaga aataattgca
     6661 gttttccaac cccatacgtt tacgcgtacg attgcattga tggatgaatt tgcagaagca
     6721 cttgatttag ctgataaagt ttacttatgc gatatttttg gttcggctcg ggaagaacaa
     6781 ggaaacgtta agattgaaga ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa
     6841 gaaaataatg tatctccatt gttagattat catgatgcag tagtaatctt catgggtgct
     6901 ggagatgttc aaaaatttga acaagcgtat gaaaaacttt taagcagcac gacgaaaaat
     6961 gtactgtaat acaattactg cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa
     7021 atcttgccaa gccccctttt ttgcaactta tgataaacgg tgttcaaaag cttgcatctg
     7081 cggtaataag cccaatcaaa tcaaaaacat gagaagctgt tttttcttgg ctgttcttat
     7141 tatttgatgc aggacagctt tttcacgacc tctgtaaact ataggaatct gttaaatcaa
     7201 gggagcagaa cttgcctttt acgctacttt tggtacaata atgtatgatt aaaaataaac
     7261 tggagggttt ctatcttgaa cataggtaag ccgagaaaac taggaaaaac aataaatgaa
     7321 atcgaggaag gcgactcgtt atccttaaca gaatctattg aagataacca gcttttgctg
     7381 tatttgggat tgacgaatga tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa
     7441 acagaatatc aacagccaat cgttccatca gtgatgctga tggggatcat cacaagtgca
     7501 atttcaaagc atcttcctgg accaggatca catgttgtca atttttctgt caattttatt
     7561 gaaccggttt tccattatga aacactgact tttcagtttg aggtcataaa agtagataag
     7621 atgaaggatg tggtgacgat ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg
     7681 gatgctgtcg ttatggtcca accgccacaa gttcctgaat tggaaatagc aatcgaaagt
     7741 gaaggtgaat tgaatgaata atccaagtac atcaatgtca ggtatcaatc gtttttatgc
     7801 aaaagtttat ggatttttag gtatgggact tgcccttagt gcagtcactt cttacttggc
     7861 tctttgggta tttcctttgc aattagcaac ttttatcaat aattttccac taggctttgt
     7921 gggtctgtgg atcgttgaac tgattttagt aatggttcta ggcgtaaaag cacaaaagaa
     7981 tccgagctta gcaatcggtg gatttattgc ctactctctt ttgaatggca taacattagc
     8041 ggttactctt gcatactacg atattggaac agtaacgcaa gctttcttga cagctacttt
     8101 gatgttcatg gggatgtctg ttgtcggagt aacaacaaaa agagatctta gcggggttgg
     8161 aagagcagga caggcagctt taatcggagt gatcattgca ctagtcttga acatcttcat
     8221 tttgagaagt agcgtagttg agctgttcat ctccctactg ttagtcgtca tctttgcagg
     8281 gattacagct tatgataacc aaagcatccg taatatctat gtccagacaa acggaacagc
     8341 acatagtgga attgctgtat tcatggcatt gaagctgtat ttggacttta tcaacttgtt
     8401 cttggcgttt ctgagaatct ttggacggaa caactaaaaa agaaaaaaat tcatcagttg
     8461 gggctatacc gaattatttg gtatggtccc gctttttctt ttcgcattcc gcaggattct
     8521 ttgttagaat gaataagaac tttacagtaa ggagttaaga agatgacaaa aaataaatta
     8581 ttactcgttg atgggaacag tgtggctttt cgcgcattct ttgcgcttca caattctctt
     8641 gaacgtttca aaaatagaaa cggtcttcat acaaatgcaa tctatgcatt caatacgatg
     8701 tttgaaaatg ttatgcaaaa agagcaaccg actcatgttc tggttgcgtt cgatgcagga
     8761 aaaacgactt ttcggacaga aatgtatgcg gagtacaaag gtggaagatc caaaacacct
     8821 ggcgaattca aagaacaaat gccatatata cgtgatttgt tgacaggact aggggtacag
     8881 tattacgaat tgcccaatta tgaagcagat gatattatcg gtactttggc agaaaaagta
     8941 gacaaagatc aatttgatgt cgtcgtgctt tcaggtgatc gggatttgac tcaattagct
     9001 agtaaagaag taaaagtgga tatcactgta aaaggtgtca gcgatatcga aagctataca
     9061 ccagaacatg tagcggaaaa atatgatggg ttgacgccaa aacagattat tgacatgaag
     9121 ggactagccg gagatgcctc tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca
     9181 gctatcaagc tattgaaaca atatggctca gtcgaaggaa tttatgaaca tatcgatgaa
     9241 atgaagcaaa gcaaaatgaa agaaaatctg ataaatgaca aagagcaggc tttcttatcg
     9301 aaaaaattgg cgaccatcaa tacttcagcg cctgtggatg tctctatcga ctcattgaag
     9361 tatgaaggga aaaatctcga taaactcgtt ccgttttata aagaaatgga tttcaatcag
     9421 ttcctttcta aattgaatat cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa
     9481 gtcgttcatg aattcaaaga ggaaatgttt actacggata tggcgttgta cgtggaaatg
     9541 atgggcgaca attatcatac agaggaaatc gtcggagtag cgtgggggac agaaaagaaa
     9601 atttatgtca cgaacgatct atctgtattc gaaaaccgag cgttccattc ttggatcact
     9661 gatccgactc ggctgaaaaa agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc
     9721 tatactggaa aaacgaaagg aatcgatttt gatgtcttgc taggtgcata ccttctcgat
     9781 acgaatgaaa aaagcacaga tatcgaagga atagctgctc attacggata caacgatatc
     9841 caatcagatg aagctgtata cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa
     9901 ctcttttttg ctcatttagc gcgaaaagta gctgcaatca atgcgttgac accaaaattg
     9961 agccaagaac tggcagataa aaaccaagaa gatcttttcc ggaaaatgga actgccattg
    10021 tctatcattt tggcagagat ggaaataagc gggatcaaag tagatgcgac tcgcttacaa
    10081 gaaatgaagg gcgagttctc tgctcgctta agagagatcg aacaaaaaat ctacgaagaa
    10141 gccggcgaag aattcaattt gaattcacca aagcaattag gagtgatctt atttgaaaaa
    10201 atgggattac ccgttatcaa aaaaacaaaa actggatatt ccacagccgt agatgtactg
    10261 gaacagcttc gtgaacaagc accaatcgta gaggatatcc tcacctatcg tcaaattgca
    10321 aaaattcaat ctacctatgt cgagggactg ctgaaagtga tccaatcaga tggaaaagtg
    10381 catacccgtt atgtccagac attgacccaa acaggacgat taagctctgt tgatcctaat
    10441 ttgcagaaca tcccgattcg attagatgaa gggagaaaga tacgtcaggc atttgttcct
    10501 agagaaaaag actggctgat ttattcttct gactattcac aaatcgagtt acgcgtactt
    10561 gcgcatatct caaatgatga acatttgaaa gctgccttcc tagaaggtca agatatccat
    10621 gccagcacag cgatgcgtgt atttggaatc gaaaaggcag aggatgtcac accaaatatg
    10681 cgtaggcagg caaaagcagt gaattttggt atcgtgtatg gcatctctga ttacggactt
    10741 tcacaaaatc taggaatcag ccgaaaagca gcacagcaat acatcgatac gtactttgaa
    10801 aaatacccag gtgtcaaaga atatatggaa agaatcgtac gcgaagcaaa agaccaaggc
    10861 tacgtcgaaa cgctgtatca ccgccgtcga tatctgccag acatcaattc aagaaattac
    10921 aatatccgtt cttttgcgga acgaacagct atcaatacac caatccaagg aagtgctgct
    10981 gatattttga aaatcgcgat gatcgaattg gataaacgat taaaagaaac aggtcttcaa
    11041 gctacgatgc tgttacaagt tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt
    11101 gaatcattag ataaattggt gaaggaagtt atggaacaag ctgtttctct tcatgtacca
    11161 ctgatcactg acagcagttg gggaaaaacg tggtacgaag caaaataaac attgtttaag
    11221 acagagcaat ataatgaagg tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg
    11281 ttcgtaaagg attggaacgg ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt
    11341 ggcccaagat catcgagcaa ccagagacac ccatctttga ggcttctctt gttggcgaaa
    11401 ccatccaatc cattggacgt agagggaagt ttttaatttt tcatctcgat cattgtgaac
    11461 tgatctcaca cttgcgaatg gaaggaaaat atcaatttac gaaagaaaat acaccaatcg
    11521 ataaacatac gcatgtactt ttcttttttg aggatggaag ccaacttcgc tacaacgatg
    11581 tacgaaaatt tggtcgtatg acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa
    11641 tcatgaaatt gggacctgag cctttgccag atagcttctt attagcagat ttcgcaaatg
    11701 gactgaaaaa gtcccacaaa gcaatcaagc cattgctgct agaccaacgt ctagtgactg
    11761 gtctaggtaa tatttatgta gatgaagcat tatgggaagc gaagatccat ccagaacaac
    11821 cggcgaacac actgcgcccc aaagaggtag aacaattacg tctatcgatc attgatgttt
    11881 tggatcgtgc aatagaagcc ggaggtacca cgattcgcag ttatttgaat gcattaggag
    11941 aatcaggcgg atttcaagta gcactgcatg tctatcagca aactggcaaa ccatgtatac
    12001 gttgtggaac gccgatcgtg aaaacaaaag tggcacaaag ggggacgcac tattgtccga
    12061 attgtcaaag gctaaaaatc gcaaagtagg atttgtttta ggtttgacgg gtggaatagc
    12121 taccggtaaa agtacggcag ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg
    12181 cgatgtgatt gccagagaaa ctgttgagcc tggaacgctt gctctgagga aaattgtatc
    12241 agtgtttggc caagaggtat tgcagcctga tgggcaacta gatcgaggga aacttgggat
    12301 gattgttttt ccatcaaagg aaaaacggca aaaacttgat cagctattag atccgtttat
    12361 ccgaaaagcg ataaaagatc aaatcgcctc tctaagttcc aatcatcctt tagtaattgt
    12421 agatatccca ttgctctacg aaggtcatta tgaccactat atggatgcag tagctgtcgt
    12481 ttatactacg ccagaaactc agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga
    12541 acaagcgcag cagagaatta gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga
    12601 tattttgttc gacaacaatg gaacaaaaga aaaacttgtg ggacaaatcg aaaattggct
    12661 ttttgctaat cgctttcttt gagtatctgt gtcaattttg ctatcctgag tatatagaaa
    12721 gtgagggata gcgatgggat cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa
    12781 agaaaaaatc ggcgagatgc tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc
    12841 agaaacagca gaagaaaaag aaaagcagta ccaagcaaga atcgatgaaa tagaagacca
    12901 gcaggcagaa gaagaagcga agcggcaaga tttgactcaa gaacgaaaaa atccgacacc
    12961 tgatcgtagt aaaacaccgc tcccagaaag tgaccacatt tccgatgagc aaaaaatcga
    13021 ggaacagcga atgaaacact atacaggaat cgaagagaag ttataaaata gtaaaaaatc
    13081 gagatgtgac ttgaagcaga tgagaaggtg ttcatctgct tcaagttttt tgtgctagat
    13141 gactaaatca gcttttgtgt aagttatttc tcagtaatca aggtgatcag tattggaaaa
    13201 ttgaaattta gtaaggacag gggacacaat tacgcatgaa agtagaaaaa attgttctta
    13261 tatccgtgtt ttttgggtag gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac
    13321 tagttgtaaa gaaagacaca tcctttatat aaaatgcgat gcaagaggtg agacaaaatg
    13381 agatgtccca gatgtcaaca taataattca cgagtaatcg acagccgtca agcagatgat
    13441 ggacgtgcga tccgtcgccg tcgcgaatgt gagaattgtt cgttccgatt cactacattt
    13501 gaacgaattg aagctgctcc gttattagta atcaagaaaa acggggaacg cgaagagttc
    13561 aatcgcgata aaattttaag aggactcatc cgttcagcag aaaaaagacc tgtagctatg
    13621 gaacaaatgg aacaaatcgt caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac
    13681 gaagtatcga cgaatctaat tggtgaatat gtgatggaag atttagttga tttagacgaa
    13741 atcgcttata ttcgttttgc aagtgtttat cgccagttca aagatatgtc tgttttctta
    13801 aaagagttgc aagatatcgt ggataaagca aaagaaaata ataaagagaa taataaagaa
    13861 aacaattaag aaacaaaaag gaggcatttc cttggaaaat gcttggaaag agcttcaacc
    13921 aaaaaatatt taccaaggaa gaaaagcggc tccgctgaga gaagacagca agaacgcctt
    13981 gctgtatctt tatcagccac taatcggcgg agaagccttg agtatctatt tcaccctgtt
    14041 atcagaaatc tctcaagaat ctggcaaagg tcctgaaggg atgcatgcag atctgcttag
    14101 tagtctaggc tgcggtatcc cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg
    14161 cttactggat gtttatttta aagaagatag tgatctggga cctcgtttta tttatgagct
    14221 gttagaacca ctcattccta aagcattttt caaagactct gttctggctt ttcttcttct
    14281 ggaaaaagtt ggtgaacgac gattccagaa gcttacccaa ttctttgaac ctaaaaggca
    14341 tacagcagag ggatatcaaa aaatcacaaa aaaattttta gatgtttatc gcttcaatga
    14401 agcagtgtac gctgctaatc aagaaaacat cgaatcgctg aataaatctt ttgaagaatc
    14461 tgaatcagta gcattacttg aagcagattc gacattcgat tggcagtttt tgaatgattg
    14521 gctggcaaaa aaacatattg caagtcttga tgatccaatc atcaaacaag tcgacatgta
    14581 tcatcagctt tacggtttcg atgaattgac acttggcgaa ctgatcagtc aatcttatga
    14641 ctttagtgaa caaaaagtat ctgaaaaaga actgcaaaaa gccattttgg caatgcagag
    14701 acgtccggtg aagcaaacaa aagaaacagt tgcggtttct gaaaacagta atgaaccctc
    14761 gtcaaacgaa catttgacaa acgcagcgct tcagctgata aaagaagcac agagcattcc
    14821 accgatgaaa tatctggaag caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga
    14881 aaactggtta ctgcaagatc ttgtttctca atcaggtctt tccagtagcg tgatcaatgt
    14941 tttgctgaat tatgtactag tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa
    15001 tacagtggca aatgaatggg cacaaaagaa aatcgccaca gcagaagaag cgatccagca
    15061 cattcggcaa ataagtaatc aagcaaaaca atcaaaacaa aagaagcaaa caaattatca
    15121 aactggcaag cgaaatgttc gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga
    15181 tgaaaaacag atcagcagcg agaaaaaagc agagattgac cgccgtttta aagaatacct
    15241 ggcaaaaaag gagggtgata tctagtggaa gacgtaggca aagaattgaa gaggatcatc
    15301 agcaaacgca attatcaaga aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg
    15361 gatgttcaag cttttctaag cgcacaccaa gaagaattga cacaagcgga tattgaaaaa
    15421 agttacgcta aattgtatga attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca
    15481 acgatgatcg ctcccggcta tgagccgaag ctgacgttga attttcatta tgtggatgtc
    15541 acttatgtac caacggaagc attgttagca cggcaaagac aagaagaaat cagaagccga
    15601 gtaaaagcaa tggatatgcc aaaagatatt caatcggctt cttttgaaaa ctttgaacgt
    15661 acagatggac gaggatatgc gagcttagaa gccctagatt ttgtagagaa atatactgct
    15721 gatccaaaag ggtatcataa aggactgtat ctagtaggaa gttttggtat tggaaaaaca
    15781 tatttattag gagcaacagc gagagaatta gcgattgctg gattcaccac gacgctagtc
    15841 cattttccga catttgccgt tgagatgaaa caggcaatcg gaaaagatca agtggcagaa
    15901 aagttagatg cagtgaaacg ttcacctatt ttgatgatcg atgacattgg tgcagatgcg
    15961 atgagtagtt ggatacgaga cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa
    16021 cagttaccaa ccttcttctc atcgaatttt acaatggatg aattggaaca gcatttgtcc
    16081 gtcactcaac gtggagatga agagccgcta aaagcgaaac ggataatgga acgcatacgt
    16141 tatttgacaa aagaaatcga aatgactggt cgcaaccgac gcaatggttg aacagttgac
    16201 aagtccaact gacatttgac ctagtagtaa aagcaggtca gcgaatcatt atatggacag
    16261 aaatgaccgg agacaatcac cgcaacaagt aatgcagaaa cttaaacgga ggatttttta
    16321 tgagggctat tttttctagt atatctgtag ttggtctatt ttttctttta gcaggctgcg
    16381 gtagcgcaga tagcaacaca ggttctgttg attcgattga tgcgggtcaa acagtaaaaa
    16441 caagcagtac gaaaacggag agacaaagca tatttgtcgg aacgattgaa agtattactc
    16501 aagtagaagc agaagaagaa acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag
    16561 atccagcgaa tatgggaacg tcattcagca atgacggagt gatcttaaat gctactgctg
    16621 aaaaaataag cgtggacata aatgaattga aaatcggtga caaagtccaa tttacattag
    16681 ttgagatgcc gatcatgacg atgtctatcc caccgcaagt tcctggagat tcgattattg
    16741 atgtttcatt gaacaaataa ccaataacat tttttcaaaa ataagaaaat gttgttttat
    16801 agaacgaaaa ggtatatttt ttaaggagat atgggaattt ttttattcct agtctcctta
    16861 ttttatttgg aaaatgctat aattatctag aaggagtgaa gctatgaacc atttagattt
    16921 atctcagcgc ttagccgcag tgggtgactt tgtccctgaa tccgctcgtt tagcagacat
    16981 tggatcagat cacgcttact tacccgttgc attgatatta agaggaaaaa tcgaatatgc
    17041 agttgctggt gaagtcgttc aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga
    17101 tggattagaa gaccgtatca ccgtacgcct tgcaaatggt ctagatgcta tccagccaca
    17161 agatggtatt acagctgtca cgattgcggg tatgggaggt tcactgatcc gagatatcct
    17221 agaagctggc aagttgaatc aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa
    17281 catcggagag cgtacattac gatcttggct gcaagaaaat cagtataaaa tccaatcaga
    17341 aaaaatactt gaagaaaata aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa
    17401 accagttact tacaatgaaa tggagctgat gttcgggcca tttctgcttg aagagaaaag
    17461 tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca
    17521 gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat
    17581 gattgaggag gttttaaagc catgacaatc agcggacgca ctttcatcca aaagtttgaa
    17641 agctattgtc ctcagtggct agctgaagaa ggagacccag tcggactgca tatcggaaca
    17701 ttggataagc caatccaacg agtaatgatg acattagacg ttcgtccaga agtggtcgaa
    17761 gaagcaatca agaaaaaaat cgatttgctg attgctaaac atcctccaat ctttcgtcct
    17821 gtaaaacggc tggttactga tcagccacaa gaaaaaatgt atgcagattt gttgaaacat
    17881 gatattgccg tctatgcagc acacacgaac atggatatta tttgggatgg actgaatgac
    17941 tggttttgtg aacttctcgg tattgaagtg gagagttatt tagtaaagac tcatgagatt
    18001 cattataaaa aactggccgt ttatgtacct gtggatcatg cacagaaaat gcgtgaagta
    18061 ttagctgcag ctggtgctgg tacacaaggt gattataccg gtacaagttt tacctcgatt
    18121 ggacacggtc gatttactcc ggaagctggt gcacaaccag cgattgggaa agtagggaag
    18181 acagaacaag ttcaagaggc aaaagttgag gttattttgc cggaaactat cgaaaaacaa
    18241 gtgattcaag ccatgcggtc agctcatcct tatgaggaac ctgcttatga tcttttcgct
    18301 atagatgaac cagtcgaaat gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt
    18361 tctatagagg catttgtaga acaagtaaaa gaagcgttcc aactagatgg actgagaata
    18421 gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg cgatttgcgg tggaagcggt
    18481 gaaaaatttt atccgcaagc aatcgcccaa agagcagatg tctatatcac gggagatatc
    18541 tattatcata cggctcatga tatgcaaagc gctgggttga ttgcaattga tcccggtcat
    18601 tatattgaat cgttatgcaa gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa
    18661 gaaaattggg atattaactt tttcgtttct gaaacaaata caaacccatt tcaatttaat
    18721 taaaaggaga gaatactatg tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa
    18781 cagaaacacg ctccgatgca acaagcaaaa cgactccatc gactcagaca caagttgctt
    18841 ttgcgcaaga attgaaaaaa gaattagaag agttaggctt agctgatgtt ttctataatg
    18901 aagataacgg attcgttatg ggaacattac caagcaatgt tgatcatgaa gtgcgttcca
    18961 ttggatttat ttcccacatg gatacagcag attttaatgc agtaggagtc aatccacaaa
    19021 tcattgaaaa ctatgatggc gagtctacga tcgtactaga tgcggaagaa cgctatacat
    19081 tgaatacaaa agattttcca aatttgaaaa attatgaagg ccacacattg atcacaacag
    19141 atggcagtac attactggga gcagatgaca aatcagggat tgcggaaatc atgactgcaa
    19201 tggagatctt gatcaataat ccttctatcc ctcacggtac catcaaagtg gcttttggtc
    19261 ctgatgaaga aatcggtgtc ggagcggata aattcgatgt tgagcaattc gatgtagatt
    19321 ttgcttatac agtcgatggt ggtcctttag gtgagttgca gtatgaaacg tttagtgctg
    19381 ctcaagcaaa tattacgatc caagggaaaa atgtacatcc agggacggca aaagatacaa
    19441 tgatcaatgc tctacagtta gcaatcgatt ttcataatca gcttccagcc gatgaagttc
    19501 cagaaaaaac ggaaggatac gaaggtttct tccatttgat ggcattggat gggaatcctg
    19561 aagaagcttc tatgagttat atcattcgcg atcatgatcg tgaaaaattc gaagcacgca
    19621 aagcacaaat cacacatatc caagaagagt tgaatcagcg atttgatcaa gaacgaatca
    19681 aagtagatat gtatgatcaa tattacaaca tgaaagaaat cattgaaaaa gatatgagca
    19741 tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat ctctccagtg atcgaaccag
    19801 ttcgtggagg aacagatggt tcaaaaatct cttacatggg tattccgacg ccaaatctat
    19861 ttgccggagg agaaaatatg cacggacgat tcgaatttgt ttctttacaa gtaatggaaa
    19921 aagcaacaga cgtgattgta aaaattgcag aattgaacac aaagagttaa aatcgaaaaa
    19981 gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag
    20041 ggcaggacaa tggaattggt tgtcccgccc tcttttttga agagtaaata ggaggtaaag
    20101 aatggaaaag ctgctagttt tttataacaa aagttcaggg aaagatgagg gtgagaaatt
    20161 agcacgctgg tttcaagatt atgtaaaaga aaagcagcca gaattatatg tagaactagc
    20221 tgaaactggc ccatcgattg atgatgcgga attagtcaag aaagcagaag ccatagaagc
    20281 tgatacttta gtaattatag gtggagacgg cacgattcat catatcgtcc aagcgttcca
    20341 aaaaacatta tctcaatacc atgtcggatt acttccaggt ggaactgtca acaaccttgc
    20401 acgtgttctt gagattcccc tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa
    20461 tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc attatctcta cactgacaat
    20521 cggaatctta gcagatacag cagtttggat cagtcaagaa gaaaaacagc gttacggctc
    20581 gtggatcttt atcaagaagt tttttaagtt attgctgaag aagaaaaaat atcatttgga
    20641 tttagaaaca gacgaggagc attggaaagg aaaatcccaa ctgttgacag tcacgatgtc
    20701 taactccgtg ggcggtttta caaattttga cgactcagcg accctagatg atggaaaatt
    20761 tcatgtaacg atcgtacctt ccttagatat tttccgattt attttttatt tgcctagaat
    20821 catgagaggc aaattctata cgatccccgg cattacttat ttcacagcca aacgcatgaa
    20881 aatccaagcg caggaaaaat ctgtgggcac tcgaacggat ggagacacga cagatgatct
    20941 tccagttgaa ttatctgttg tatcaaaagg attaacagtt tattcaccaa agaaagaaga
    21001 aaaagcttag aaatagtagt taacagaaag ttggaagaag agtaatggat gtattgttac
    21061 acaatctttt gtggttttac cttttcattg tgaattttta cttattttgt ctgatggggt
    21121 atgataaaca tcaggctaga actagcggct ggcgtgttcc tgagaataat ttgatatttg
    21181 tcagcctgtt aggcggtggt ttgggaggat tgatctctcg aaaagttttc catcataaaa
    21241 ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt acttgttgat ttgattctca
    21301 tttatttttt ccattaagga gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt
    21361 ctgaatctgc ttttgctgat tgtgattttt gccattattt attatttaat caatcaatca
    21421 tttgcagata tctttgccga attgctgtct accagtgttg ttgttttggc agcggctgtt
    21481 atattaggga cagtttacca aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct
    21541 ttcgcgccga attttacaac gaaagatggg ttcttgacct ctagctatat tgcgttttac
    21601 cgaatcgttt cgtttggtac aggaacgctg atctcggaga tttattttta taacaaaaaa
    21661 ggaatgaagc tatcccaggg agcaggggtg acagcactcc atatgattat gtacaaactg
    21721 gcagtgatca cttatgcatt agctggcttg gtcatccaat tctctctttt ttattctaat
    21781 ggtccatcaa tgatctggtt tatcatcgct ggtatccttt tgacgatcgt gattgttgcc
    21841 tttttattgg cgatgtccgt cagcttgaat gttcaaatat tttttgtcag cttatcgaat
    21901 aagttattta aaagtgagcg tttgcggaaa tgggtggata catgcaatca gcagatctac
    21961 tctttaaggg aaacggtcca aaccattatc caagacagga ctgcgctatt gcggatttat
    22021 ctatggaata tgttaaaact tgtattttgg tatgtacttc cttatttgat tctgattgaa
    22081 aaccatccta aaatagattt tctactgacc atttcgttta tcagctttgc agtagtgctt
    22141 tcaggggtta ttcctacgcc tgctggtatt ggttcctttg aatttgtcta tttgttatta
    22201 ttcagaccag ttgttggaac agttgacgca gtttcttcgc ttttactgta tcgtttcagt
    22261 tcctttatct tgccatttct ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg
    22321 atcaaagaag aaataataga agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt
    22381 tataagatct acccttttta tcaaatcaaa gaaaaacgtt atggataaga taattttacg
    22441 tttgtttggc tcttttttta tatacataca atcaattttg tttttttaag atttatataa
    22501 gtcgagaaaa aaagtcaaaa aaccatgtgt atattttgac caaaattgac caaagcgata
    22561 taatagtttt tgtaaagatc aaagaaataa gtgatcttac ctttaattag gagggtatac
    22621 ctgtatgaat attgagaaaa tgactacgac gcttcaagaa gcgattgctg aagctcaaca
    22681 gattgcggtc actcgaaaac atcaagatat cgacattgcc catgtatgga aaatcttttt
    22741 acaacctaat cattttggtc gaaatttcta tacggatgcc ggattggatg tcgaatcttt
    22801 tgaacatgaa attgatcgtc tactggatga gtatccagtt gtttctggtg gaaacgtcca
    22861 atatggacaa aacctcagcc agaatttctt tcgattattg aatgaagcag accaaatacg
    22921 tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt
    22981 aaaaaattat ccgttaactg tttatttgaa taaaaacgga ttatctgaaa aagaattgcg
    23041 taaaaacatc gaagagatga gaggaggaga aagagtgacc tcacaaaacc aagaagaaca
    23101 atacaaagca ttagaaaaat acggcgttga tttagtacag caagtcaaaa gcggaaaaat
    23161 ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa
    23221 aacaaaaaat aatcctgttc tgataggaga acccggtgta gggaaaacgg caatcgttga
    23281 agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat
    23341 tttttcatta gatatgggtg cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga
    23401 aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat
    23461 cgatgaaatc cacaatatcg taggggcagg aaaaacagaa ggaagtatgg atgccggaaa
    23521 tctgttgaaa ccaatgcttg ctcgcggtga attacatttg atcggtgcca caactttaga
    23581 tgagtaccgt caatatatgg aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt
    23641 agtcaaagaa ccaacagtgg aagatacgat cagtattctg cgtggattaa aagaacgttt
    23701 tgagatccac catggtgtta acatccatga caacgcatta gtagcagcag ctactttatc
    23761 tgaccgttat attactgatc ggttcttacc ggacaaagca atcgatttga tcgatgaagc
    23821 cagtgcaacg attcgggtag aaatgaactc tatgccaacg gaattagatc aagttactag
    23881 acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag
    23941 taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc
    24001 gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc
    24061 tgaaatcgat aaagcaaaac acgaattaga agatgcagaa aataactatg atttagaacg
    24121 cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa aaagaattga aggaattaga
    24181 agctaaagca aaagacagcg agattaagat ggttcaagag tccgttaccg aaaatgagat
    24241 tgctcaagtc gttggcagat taacgggtat ccccgtaacg aaacttgtag aaggtgaacg
    24301 agaaaaacta attaaattga atgaaacact tcataaacga gtgattggtc aagatgaagc
    24361 agttgatgcg gtcagtgacg cggtaattcg ttcacgggct ggattgcaag acccaaatcg
    24421 tcctctaggt tctttccttt tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa
    24481 agcgttagct gagaacttgt ttgactcaga agatcatatg gtacggatcg atatgagtga
    24541 atatatggaa aaacatgctg tgtctcgtct agtcggagct cctccaggct atgtaggtta
    24601 cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt
    24661 ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc ttgttacaag tcctagatga
    24721 cggacgcttg actgattcaa aagggcgagt agtagatttc aaaaatacgg ttctgatcat
    24781 gaccagcaat atcggttcac agttactgct ggaaggcgtg actgctgatg gaacgattcc
    24841 agaagcagtg gctgaacaag tcaacacgct attacgtgga aacttcaaac cagaattctt
    24901 gaaccgaatc gacgacacga ttttatttac accattgagc ctagacaacg tgaaaggaat
    24961 cgttgacaaa atggtggcgc aattggcaca acgtctggaa catcaagaaa tcctgctgac
    25021 aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct tatgaaccag catatggtgc
    25081 aagaccattg aaacgattta tcactaagga agtagaaaca ccactagcta aagaaatcgt
    25141 tgcaggacat gttatgccta aatcaaaagt aacgattact ttacttgatg gacaacttca
    25201 tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc attgagacta taaaagaaaa
    25261 aaggggtctg acacaactag tgtcagacct ccttttttca aataaacagt gttccagaag
    25321 tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct
    25381 tttattaggt atttttcact ttttctaatt tataactttc aaagtaacgc tcatcttttt
    25441 tactttgaga ccatgcaaac aatcccatta aggcagcacc tatcgtattt gtcagcaagt
    25501 cgcccatcgt atccatcaat gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa
    25561 aacgttgaag attcatgcct aagaactggt cgcataagaa ttcccagaac tcccaaaaca
    25621 cgccgcaaag tccagcaaac gcaaagccga ataagaggaa caaccaagga ctggtttttg
    25681 atatctcagc atctttcatc aagtaaccaa tcaatccata gccaagagct gtcaggacca
    25741 ttggactcac cgcatgtaag attttgtccc aaaaagagat gatactgatc aaatgcatcc
    25801 ctgtgcctaa aaagacagag atgaacaaga aaaaccagta aaacaaaacg attgctgctg
    25861 gtattttaag tttgaaaact ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta
    25921 ataaagattc cacgataaat gtgattcctt gagaagtcga atatttttgt gtgataaatt
    25981 cataaaaatt atagcagaca acgaatacac caaaagcaag taaagctatt tgcacctttt
    26041 tgatttcttt aatcactgaa agtccctcca tctaaaaatc tttttacttc tgaaagcaac
    26101 tcaatttctg aagaaaccac ttttccgttc aatttgatca aacctattgt atataagttc
    26161 aggtagtgaa attgattttc agctgtttct tgtagtgcag cgattttttt cgggtgggtt
    26221 ccgccttgct gtcgagtatc agtatataag ccgatcactg ggatgccttt cgcataagcc
    26281 acaccgattt ccgatgctac cccggcatcg atcgtcagcc catctagtaa agcaatcatt
    26341 aaatcactag ccaacacttt ttctgtgtca gcaagagcaa tcattcgact gtctgcataa
    26401 gcagttttgt cattgattgc ttcattttct tgaggaagat agatcgaaag ctcagaagag
    26461 agctctctga tttctttaac taactgttgg ttatataaca aatcactttt agcaaacatt
    26521 ggtgcggcaa aataaatttt catctaaaca tcatcctttt tatcaaaaat aatcgctaaa
    26581 gtaagcttcc atgtttggaa tgatacgctc tagtgacttc agtgtttctt catctgtttc
    26641 taaccgttcg attcttgcca aataagaagc acggcgataa ttcatcatca agcaagtcaa
    26701 tgtttggata tccagtcgaa cggattttcc tataggttca tttaagacac ggacttgtcc
    26761 ctgttcatcc catgttaaag cgaagacacc attattccaa ggagcgacag gatcttcaat
    26821 gatgaaatga aaagcatcag ctgttccaac aaacggaaac cgttgaagga attcttttac
    26881 atcaacgatc cgagccataa aatatggttc gatctgctct ttgatctgac tatcttcaat
    26941 caaaaatgct aaaggctcat ttttataaat atctccatgt acccagtaga tcatggaaaa
    27001 atgtgcggaa ataaagttcc ataagccatt tcgagcttct tgatcaagat agaacatttc
    27061 ttttacgtga aatatttctt cagctaccca gtagaacaga acacctcttg gttcatggtt
    27121 gctatcgtag tagacagcgg ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc
    27181 ccaatgaaaa tcactgcgga ataaagcacc atggttttgt tgggcaaatt tgtcataaac
    27241 ggaaaatacg tctggatgat caactggtaa tcgctcaacc ataccactta ctgggatttg
    27301 tttaggtagc tgtgtatctc tgattttaaa ggaaagttta tcagacatga tttcccatcc
    27361 tttacgccga tagtagggga tactgtaagg atacaaataa gagatccatt gcttattctt
    27421 gcgcatatta tccaatgcaa cgataattag atcttgcatc aatccatggt tagcatattc
    27481 tggataagtt ccaactccag tcacaccacc cattttataa cgtgcaccgt gaatattgac
    27541 ttcacaagga tagatagcga tttgagagat cagtttattt tcgtggaacc atccgaacac
    27601 tttcgatact tccaaaatcg gttttttgga acggataaaa gctcgtttat tctcaaaccc
    27661 gctttcctcg atgtctgctt cagtcacttg gaacacatag gaaagcaatt cattgaattg
    27721 atcgatatgt tcttgagtga ccggttttaa ttcaagattt tctctaaaat ctttttgatc
    27781 cattttttca tctccattta ttagcttgat aacagtatag caaaattcta caaaaaaaac
    27841 ttctatagct gactaattaa aaactcttta aattactgct tttcgttggt ataatggaaa
    27901 gtgctgatta tatgaaaaga ggagacgcta tggatataaa gaaaatgaaa gaacgccaag
    27961 agcagattcg taatttttcg atcatcgctc atatcgacca tgggaaatct acattggccg
    28021 atcgtatttt agaaatgacg cacactgtga cgtctcgcga aatgcaagat cagctgttag
    28081 actcgatgga tttggaacgt gaacgtggaa tcacgattaa attaaatgct gtagaactca
    28141 attacacagc gaaaaatgga gaaacatata tctttcactt gattgacacc ccagggcacg
    28201 tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg
    28261 tcgatgcagc acaaggaatc gaagcacaaa cattggccaa tgtttattta gcgttagaca
    28321 atgatttgga gatcctgcca gtcatcaata aaatcgacct acccgcagca gatcccgaac
    28381 gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc tagtgaagct gtattagcca
    28441 gtgccaaatc agggattggg attgaagata ttctcgagca gatcgtggaa tatgttcctg
    28501 caccaagtgg agacttagat gcaccgctaa aagctttgat ctttgactct atttatgata
    28561 gttaccgcgg tgttgttcta aatgtccgga tcacggacgg tatggtcaaa cccggagaca
    28621 aaatcaaact gatgagtaat ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc
    28681 caaaagcagt tgctagagac tttttgatgg tcggtgatgt cggttatatc acagcaagta
    28741 tcaaaactgt ccaagataca cgagtagggg atacagtaac gttagcagac aatcctgccg
    28801 ctgaagcttt accaggttac cgaaaaatga atccgatggt ttattgtggt ctttatccaa
    28861 ttgatacttc tcgttacaac gatttgcggg aagctttaga aaaactgcaa ttgaatgatg
    28921 ctgctttaca attcgaacca gaaacctctc aagcattagg ttttgggttc cgctgtggat
    28981 tcctcggact tcttcatatg gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag
    29041 aattgatcac gactgcacca tccgttattt accatgttaa taaaacggat ggtacgactg
    29101 ctacagtaga taacccggca gattttcctg aacctgtaac gattcaggat gtagaagaac
    29161 cattcgtcaa agcacagatc atggtcccaa atgattttgt cggtgcagta atggaacttt
    29221 ctcaacgaaa acggggagaa ttcattacga tggattattt agatgattac cgagtaaatg
    29281 tcgtctataa tattccgctt tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa
    29341 gcacgaaagg ctatgcctca ttggattatg aaatgtctgg ttatcaaaaa agcaagttag
    29401 tgaagatgga catcttgctg aatggagaaa aagtcgatgc attaagcttt atcgttcacc
    29461 gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac
    29521 gccaacaatt tgaggtgcca atccaagcag ctattggaca aaaaatcgtt gcccgttcag
    29581 acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta tggtggagat gtttctcgta
    29641 aacgtaaact cttagagaaa caaaaagaag ggaagaagcg gatgaaacaa atcggctccg
    29701 tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga
    29761 aatagcaagg gtttgtttga aaacagtgta aaaaataaac catgtttaca tggatttgcc
    29821 accggtttgc caccggtggt ttttttcggt ggcaaaaatc aagaattctt tctaagattt
    29881 tccatgatat cgaccgtttc atttctcatc ttatctgtta catgcgagta agtatccatc
    29941 gtaattgaaa ttctgctgtg gcccaatcgt tcagagattt ctttcatttt tgcgccattt
    30001 tcgagaagaa gtgtagcatg agtatgtctg agagaatgga agttaaaaga gagggagagt
    30061 gcattcgata tcctcctagt attccatttc actacacttg gcgtaactag ctcaccgtct
    30121 tccttcgtac atactgcatt tgaatcaatg tagagctttc catacttcat tcgattttct
    30181 aattgttgtt tcttatgttt tttcagaatt gctagcaagg tttgtccaat aaaaatcgtt
    30241 cgattggaac tgcttgtctt tggcgtacca tatacccatg cgccgtcatt ctttaccatt
    30301 tgtttctcta cagtaattgt tccatttgaa aaatcgacat catcccacgt cagaccacaa
    30361 acttcgccaa cgcgcattcc cgtataaaat ccaatattca aaggaatata gaaaggatgt
    30421 ccttcaggag taatttctag catatgatca aagtcctcaa gagaaatgat ttttagatct
    30481 tttttagtcg ttggtcgtgc ttcatatttt ggtattttta catacagcat aggattttgc
    30541 ttgattaatc cccaaggata aaccgccata ttcagcgcat tcttaaggac agagtgagta
    30601 atagtcattg ttttcttcga gtaacccttt ttaaattcag cattgatgaa attttgtaaa
    30661 agagcagggg agagatccgt aagttttttc tttcctaaat aaccgtttat atgatttttg
    30721 atggtaaatc ggtagttttc ataggtattg tattttagat ttagtttaac gtattcctcc
    30781 atccaaaaat caaggtattg ttttactcga gtatccgtac ctaaaaagta ttgtcctgtt
    30841 tcgtcaatat ctgataaaac ttttcgtaaa gcagcttcgg cctctggtcg ggtgtctccg
    30901 ccaactttct ccactttttt tcttgagcca tcatcattga tatct
//
LOCUS       DAFFFQ010000024        29154 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000024
VERSION     DAFFFQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.024"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..1030)
                     /locus_tag="LQB69_001214"
     CDS             complement(<1..1030)
                     /locus_tag="LQB69_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3525390.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="LQB69_001215"
     CDS             complement(1112..1801)
                     /locus_tag="LQB69_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3525391.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LQB69_001216"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="LQB69_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL3525392.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="LQB69_001217"
     CDS             complement(2328..2831)
                     /locus_tag="LQB69_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL3525393.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LQB69_001218"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="LQB69_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL3525394.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LQB69_001219"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="LQB69_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL3525395.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="LQB69_001220"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="LQB69_001220"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL3525396.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="LQB69_001221"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="LQB69_001221"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL3525397.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="LQB69_001222"
     CDS             complement(6443..7462)
                     /locus_tag="LQB69_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL3525398.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="LQB69_001223"
     CDS             complement(7635..8540)
                     /locus_tag="LQB69_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3525399.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LQB69_001224"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="LQB69_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL3525400.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LQB69_001225"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="LQB69_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL3525401.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LQB69_001226"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="LQB69_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3525402.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="LQB69_001227"
     CDS             9753..10397
                     /locus_tag="LQB69_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3525403.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="LQB69_001228"
     CDS             complement(10453..10956)
                     /locus_tag="LQB69_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL3525404.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="LQB69_001229"
     CDS             11553..12518
                     /locus_tag="LQB69_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL3525405.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="LQB69_001230"
     CDS             complement(12617..13789)
                     /locus_tag="LQB69_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525406.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="LQB69_001231"
     CDS             complement(13776..14921)
                     /locus_tag="LQB69_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525407.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="LQB69_001232"
     CDS             complement(14935..15867)
                     /locus_tag="LQB69_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525408.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="LQB69_001233"
     CDS             15984..17063
                     /locus_tag="LQB69_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL3525409.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="LQB69_001234"
     CDS             17060..17689
                     /locus_tag="LQB69_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3525410.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="LQB69_001235"
     CDS             complement(17799..18026)
                     /locus_tag="LQB69_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3525411.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="LQB69_001236"
     CDS             complement(18129..20717)
                     /locus_tag="LQB69_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL3525412.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="LQB69_001237"
     CDS             complement(20737..21396)
                     /locus_tag="LQB69_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3525413.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LQB69_001238"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="LQB69_001238"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL3525414.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="LQB69_001239"
     CDS             complement(22169..23035)
                     /locus_tag="LQB69_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3525415.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="LQB69_001240"
     CDS             23144..23785
                     /locus_tag="LQB69_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL3525416.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LQB69_001241"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="LQB69_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL3525417.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="LQB69_001242"
     CDS             complement(25226..26125)
                     /locus_tag="LQB69_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL3525418.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="LQB69_001243"
     CDS             complement(26127..26924)
                     /locus_tag="LQB69_001243"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL3525419.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="LQB69_001244"
     CDS             complement(26902..27606)
                     /locus_tag="LQB69_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL3525420.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="LQB69_001245"
     CDS             27737..28312
                     /locus_tag="LQB69_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL3525421.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="LQB69_001246"
     CDS             28430..29107
                     /locus_tag="LQB69_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL3525422.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8582 a         6173 c         5242 g         9157 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa aggctctttg tcaaatgatg ttggtaaaga gaaatcttag aata
//
LOCUS       DAFFFQ010000025        29046 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000025
VERSION     DAFFFQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.025"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(105..629)
                     /locus_tag="LQB69_001247"
     CDS             complement(105..629)
                     /locus_tag="LQB69_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525423.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LQB69_001248"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="LQB69_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL3525424.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LQB69_001249"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="LQB69_001249"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL3525425.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="LQB69_001250"
     CDS             complement(2351..3031)
                     /locus_tag="LQB69_001250"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL3525426.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="LQB69_001251"
     CDS             3157..4026
                     /locus_tag="LQB69_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3525427.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="LQB69_001252"
     CDS             4052..4567
                     /locus_tag="LQB69_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525428.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="LQB69_001253"
     CDS             complement(4614..5207)
                     /locus_tag="LQB69_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525429.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="LQB69_001254"
     CDS             complement(5232..5411)
                     /locus_tag="LQB69_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525430.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="LQB69_001255"
     CDS             complement(5488..5997)
                     /locus_tag="LQB69_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525431.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="LQB69_001256"
     CDS             complement(6093..6473)
                     /locus_tag="LQB69_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3525432.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="LQB69_001257"
     CDS             6803..7552
                     /locus_tag="LQB69_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL3525433.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="LQB69_001258"
     CDS             complement(7736..9013)
                     /locus_tag="LQB69_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525434.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCHYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LQB69_001259"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="LQB69_001259"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL3525435.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="LQB69_001260"
     CDS             complement(9935..10879)
                     /locus_tag="LQB69_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL3525436.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="LQB69_001261"
     CDS             complement(10881..11567)
                     /locus_tag="LQB69_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL3525437.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="LQB69_001262"
     CDS             12041..12361
                     /locus_tag="LQB69_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL3525438.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="LQB69_001263"
     CDS             12515..13420
                     /locus_tag="LQB69_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525439.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="LQB69_001264"
     CDS             13417..14190
                     /locus_tag="LQB69_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3525440.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="LQB69_001265"
     CDS             complement(14371..15795)
                     /locus_tag="LQB69_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3525441.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="LQB69_001266"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="LQB69_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL3525442.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="LQB69_001267"
     CDS             complement(16517..18499)
                     /locus_tag="LQB69_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3525443.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LQB69_001268"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="LQB69_001268"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL3525444.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="LQB69_001269"
     CDS             complement(19412..20473)
                     /locus_tag="LQB69_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3525445.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21250)
                     /locus_tag="LQB69_001270"
     CDS             complement(20684..21250)
                     /locus_tag="LQB69_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3525446.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LQB69_001271"
     CDS             complement(21278..22780)
                     /gene="eat(A)"
                     /locus_tag="LQB69_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL3525447.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="LQB69_001272"
     CDS             complement(23038..24159)
                     /locus_tag="LQB69_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL3525448.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="LQB69_001273"
     CDS             24285..25226
                     /locus_tag="LQB69_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL3525449.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="LQB69_001274"
     CDS             complement(25376..26272)
                     /locus_tag="LQB69_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL3525450.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="LQB69_001275"
     CDS             26541..27881
                     /locus_tag="LQB69_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3525451.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="LQB69_001276"
     CDS             28099..28542
                     /locus_tag="LQB69_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL3525452.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT         8365 a         6015 c         5220 g         9446 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagtg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaacgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat tccaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttt
//
LOCUS       DAFFFQ010000026        28684 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000026
VERSION     DAFFFQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.026"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(23..1189)
                     /locus_tag="LQB69_001277"
     CDS             complement(23..1189)
                     /locus_tag="LQB69_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3525453.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(1201..1554)
                     /locus_tag="LQB69_001278"
     CDS             complement(1201..1554)
                     /locus_tag="LQB69_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL3525454.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            1682..2704
                     /locus_tag="LQB69_001279"
     CDS             1682..2704
                     /locus_tag="LQB69_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL3525455.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            2723..2884
                     /locus_tag="LQB69_001280"
     CDS             2723..2884
                     /locus_tag="LQB69_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525456.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(3143..3577)
                     /locus_tag="LQB69_001281"
     CDS             complement(3143..3577)
                     /locus_tag="LQB69_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525457.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(3687..5546)
                     /locus_tag="LQB69_001282"
     CDS             complement(3687..5546)
                     /locus_tag="LQB69_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3525458.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(5664..6823)
                     /locus_tag="LQB69_001283"
                     /pseudo
     CDS             complement(5664..6823)
                     /locus_tag="LQB69_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7130..8554)
                     /gene="araA"
                     /locus_tag="LQB69_001284"
     CDS             complement(7130..8554)
                     /gene="araA"
                     /locus_tag="LQB69_001284"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL3525459.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(8570..9268)
                     /locus_tag="LQB69_001285"
     CDS             complement(8570..9268)
                     /locus_tag="LQB69_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL3525460.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(9225..10886)
                     /locus_tag="LQB69_001286"
     CDS             complement(9225..10886)
                     /locus_tag="LQB69_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL3525461.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            11104..12504
                     /locus_tag="LQB69_001287"
     CDS             11104..12504
                     /locus_tag="LQB69_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL3525462.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(12756..13835)
                     /locus_tag="LQB69_001288"
     CDS             complement(12756..13835)
                     /locus_tag="LQB69_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3525463.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(13921..14880)
                     /locus_tag="LQB69_001289"
     CDS             complement(13921..14880)
                     /locus_tag="LQB69_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL3525464.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(14892..16403)
                     /locus_tag="LQB69_001290"
     CDS             complement(14892..16403)
                     /locus_tag="LQB69_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL3525465.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(16425..16988)
                     /locus_tag="LQB69_001291"
     CDS             complement(16425..16988)
                     /locus_tag="LQB69_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL3525466.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(16994..17857)
                     /locus_tag="LQB69_001292"
     CDS             complement(16994..17857)
                     /locus_tag="LQB69_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3525467.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(17871..18767)
                     /locus_tag="LQB69_001293"
     CDS             complement(17871..18767)
                     /locus_tag="LQB69_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3525468.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(18942..20231)
                     /locus_tag="LQB69_001294"
     CDS             complement(18942..20231)
                     /locus_tag="LQB69_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3525469.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(20203..21720)
                     /locus_tag="LQB69_001295"
     CDS             complement(20203..21720)
                     /locus_tag="LQB69_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL3525470.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            21918..22808
                     /locus_tag="LQB69_001296"
     CDS             21918..22808
                     /locus_tag="LQB69_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL3525471.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="LQB69_001297"
     CDS             complement(22881..23705)
                     /gene="nadE"
                     /locus_tag="LQB69_001297"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL3525472.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(23717..25189)
                     /locus_tag="LQB69_001298"
     CDS             complement(23717..25189)
                     /locus_tag="LQB69_001298"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL3525473.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(25826..26188)
                     /locus_tag="LQB69_001299"
     CDS             complement(25826..26188)
                     /locus_tag="LQB69_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL3525474.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            26411..27163
                     /locus_tag="LQB69_001300"
     CDS             26411..27163
                     /locus_tag="LQB69_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3525475.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(27303..28226)
                     /locus_tag="LQB69_001301"
     CDS             complement(27303..28226)
                     /locus_tag="LQB69_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3525476.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(28241..28513)
                     /locus_tag="LQB69_001302"
     CDS             complement(28241..28513)
                     /locus_tag="LQB69_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525477.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         8478 a         5987 c         4883 g         9336 t
ORIGIN      
        1 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
       61 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
      121 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
      181 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
      241 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
      301 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
      361 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
      421 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
      481 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
      541 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
      601 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
      661 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
      721 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
      781 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
      841 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
      901 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
      961 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     1021 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     1081 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     1141 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     1201 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     1261 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     1321 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     1381 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     1441 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     1501 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     1561 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     1621 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     1681 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     1741 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     1801 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     1861 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     1921 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     1981 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     2041 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     2101 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     2161 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     2221 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     2281 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     2341 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     2401 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     2461 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     2521 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     2581 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     2641 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     2701 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     2761 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     2821 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     2881 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     2941 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     3001 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     3061 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     3121 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     3181 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     3241 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     3301 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     3361 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     3421 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     3481 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     3541 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     3601 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     3661 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     3721 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     3781 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     3841 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     3901 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
     3961 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     4021 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
     4081 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
     4141 gccgtgctgc tataggaaat atcttgcaga taattctcta agtcaagtgt cccacttcca
     4201 tctgaagttt ttgcaggata gtctagatta tcatactttc catcgtaggg catatgtgtc
     4261 tgcatcgtga ctaaatgaat gaactgaggt gttttttctt ccttgagaag tgtcattact
     4321 tctttgtaag ctgactcatc agaaatatat ggattattct ctatcgtatc cgtataggtc
     4381 atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct gataaacatc ctctctttta
     4441 tacatcgaag tattgtatgg atggatcgct gtcgtctggt atgattgtgc gtttaacgct
     4501 gaaacgatag aaggaagctg atccattttt ggtacaagca tcgtgtaagg ggtggtgagt
     4561 tgcgcattaa ataacgccat agaaaaacca gtcaatgctt caaattcgat atttgctgtt
     4621 ccaccgccat agttttgaga aagcatattg ccactatatg tttgatcagc tacttcataa
     4681 taatctgcca atggctcacc agatacttct acgccgttta atcgactagg atctgagaaa
     4741 ctttcactca tcacgaacac gatgttcggc gactcttcct ctacagtttt atttttttca
     4801 tcggctagct tctggtattt ttcagtgatt tctttgatct tttctttcga atatccttca
     4861 ggttcatcca ttggttctac cttcagattg tacaaaaacc caccgataaa accagtgtta
     4921 tagtagttca ttttctggct gtaaggtatc catagtgccg ttttattata tgcttttcgc
     4981 aacaaattat cgggattgtt gaaattactg atatacccta gcagtccaat tgccacaagc
     5041 agtgtcgtta atcggatcaa ttggaatcgc cttccttttt tcaggcttcg aaacatgtac
     5101 cagcaaaaca atcctatcac ggttcctgcg gcagctaaga tcactgtaaa cagcatagtg
     5161 ccggtcatat ctttcagcaa tccgatttca gtgatcatct tcaaatcgtc tggataaatc
     5221 ggttcttgcc gataactcat tttcatataa tttgcaaaac ctaatacacc gatggctacg
     5281 acatacgtaa gggaacctaa agggacagat cctgctagtg caatcagaaa aataagaata
     5341 atcagcaaga cgagacaggc taagaagaat ttttccgtat gccaagaaaa agcaaatttc
     5401 aacgcaaggt ctacggatag atcattttga caccactgca aaaataaatt actaaaaaca
     5461 acaagaaaaa agatactgat gattgctgct gttcctttta aaatcttttt ctgtttggtc
     5521 gaaagtctgc ttatatatct tttcaaatgc ttgctccttc atttctatca ataaaagaat
     5581 gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa agaacggaaa gaagaattaa
     5641 tgaaatccaa atattctctt tctttatggc ctttctggtt tctttaataa gcggaccttt
     5701 cgtactccag aaattttttc tagctgttgt ttcacttgat tgagctgttc aaaatcagta
     5761 ctgtctatgt caatcaatat gtagacatgc tcattcttat ggttacgcga tatatctgcg
     5821 atcgctagct cgttctcatt cagcagagaa aatatctctg ctaatatatt ggttttcttt
     5881 tgataaaata tagtgaatcg aaacggcgct tggaaaaaca ttcgtgcgga tggataattg
     5941 accgcttctc taaccgtacc gaacaaaaga taatctttta aggcttcaac tgccaaacgt
     6001 ccgctgtctg ccaaagcttc ctgagtcgat cccctagatg aggcaacata agtatacggt
     6061 cattttctaa gaactcttct tctggaaaat cagtcaagta tttagccaga tgttgttttt
     6121 ccaatgcttg cataacagca tatttatcaa caatttcact tcttcctaca ttgataagta
     6181 cagcatcttt tttcattttt tctatattcg cctgatcgat caatcctttt gtatcttctg
     6241 tcaacggcag catgactacg atgtaatccg aggtagaaag aactgtttct aaatctgctt
     6301 gccgaacata atccaactgg gcatcgcgga tagaataacc caagacatcc atccctaagc
     6361 tatagcaact tagcgctact tttgtgccta tcgctccaag accaagaaga ccgatggttt
     6421 tgccttgcaa ttctcgtccc acataagcac ttcttttgtt ttctgcctgt tctaaaatat
     6481 taggacctgt tagtgtttgt accatacgac ttgcttcgat tatcggacgg gaagataaga
     6541 gcaaacagca caaaacaagc tcttttactg cattagcatt tacacctggt gtattcatga
     6601 cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt gttcacccca acaccagctc
     6661 ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt cactttcgta ctacgaataa
     6721 ctattccttc ggctggcaaa tcatctatta ttttgaaata ttccccttgt aacttttcta
     6781 tctcttgttc tggaatagca tcaatcaaat gaacgtgata cataaaaccc cctccttact
     6841 aacaagtatc tcgaaatttc tgcaattaga caactgttta ccatttgact tcctagaagc
     6901 aaaaagagca gttagagcag agtaggctga gtgataaaaa taaggctatg acaagttact
     6961 gccataagcc ttattttcga gagcaaaata ttgagggtga gatatcttga taaatggtgt
     7021 tccgttagct gacattgata ataagactaa atttcaaaaa agttgaaaag caaatttcgt
     7081 caatctcttc ttatttctta aaactaagtg gctttttcac aacctctttt tatctaataa
     7141 ccactgtttc caactcaact aattttgccc acgtttcaat ttgatctgtt gttagattta
     7201 gcgagacaac ggtatgatga ccgccacctg cctgaatcca tgaatggaca ccttcatgga
     7261 aattcggttt agttttccat aacacacgtg caacaggtaa attaggtgct tcagtcgttg
     7321 gttcaaaggc ttctacttca ttgatcagca gtttataatg cgtaccgaaa tctgccatcg
     7381 atacgactac cccttctcct gctttcccat caaatacaag acgcgccgga tcttctctat
     7441 ctcccattga taaaggagaa acaacgattc ttggtttcgt cgcagcaagc gtcggatcta
     7501 cttccatcat atgggattcc aagattgctt cttggccact agctagctcg taagtataat
     7561 cttccatgaa tcctgtatct ttccctcgag ccatgatttt cagcaaccga tcaatcgctg
     7621 cagttttcca gtcgccttcc ccagcaaaac cgtatccttc tgccatcaaa cgttgtacag
     7681 ctagtccagg tagttgtttc attccataca ggtcttcaaa attagtcgta aaagcgttat
     7741 agcctccttc ttctaagaaa cggcgaatac cgatttcctg ctgagcttgt acttttacat
     7801 gttcctccca tgtcttttct tcataatcac catagtcgaa ggtatacaat tccttgtact
     7861 cttcaaataa ttgatcgact tcttcgtctg atacgcgatc gattacttgt acaagatctc
     7921 cgataccaaa ataatcaaca gtccaaccaa attgaatctg tgcttccact ttgtctcctt
     7981 ctgtgacacc cacgtttctc atgttgtcac caaaacgagc cacttttatt ccaaaacttt
     8041 cattataggc tactgcaaca tccatccaat cagcaatctc tttttggatc tcaggtcgtt
     8101 tccagtagcc aacgacgatt ttattttgtt tatttaatcg agcattgatg aacccatatt
     8161 cgcggtcacc atgagcggat tgatttaaat tcatgaaatc catatcgatc gttttccaag
     8221 gaatgctttc gttatattgc gtagctaaat gaagcaatgg tttttgtaga agctttgtcc
     8281 cgcggatcca catttttgct ggtgagaaag tatgcatcca agtgatcaca cctgctactt
     8341 cttctcgata attgacctct ttcatgattt ttgtgatcgt atcagcagta actgctaatt
     8401 cttgaaggcg gattggataa ggcaattggc cattttcatt cagttcatcg accatttctt
     8461 gtgcatgttt ctttacttct cgcagtgctt cttccccata taaatgctgt gatcctacaa
     8521 caaaccaaaa ttctttttct ccaatattca acataattag ccactccttt tatttgtttt
     8581 gtccgtaata cgcatttttt ccgtgttttc gtaaataatg tttatctaaa atccgttgag
     8641 gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa attcatcttg catacttcat
     8701 ctagtacgac tgcgttcatt accgcacttt gcgcatcttt cccccaagta aatggaccgt
     8761 gtccgtgtaa aagtacccct ggaattgcta agggatcaat tttgcgttct ttgaatgttt
     8821 ctatgataac ttttcctgtt tcatattcgt acccgctgtc gatttcctgt tgtgtcaaaa
     8881 atcgagcaca aggtaccgat ccataaaaag tatctgcatg cgtcgtcccc atcgcaggaa
     8941 catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga gtgagtatgg acgattcctc
     9001 cgatttgagg aaatgaacga tataaaacag catgagtagg cgtatccgaa gagggattga
     9061 gtttaccttc aatgatatga ttgtccaagt ccacaacgac catgtcgtct gctgtcatct
     9121 cttcataact gactccgcta ggtttgataa cgaaatatcg tgtttcagga tcaaaagcgc
     9181 tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg caagtcaaga tttgcttggt
     9241 atacttcttc tttcagttgt tccagcattt tctatcactc ctttttcttg caattgatga
     9301 atcgctgttt tttcaatgtc gatcccctca cggtaacgtc tcataaatat ctgatagcct
     9361 gctatttctt ctgctgaagg tgtgatggtg attccttggt cttgtccaaa cacgtcttct
     9421 gccaagaagt tttctaaaga cttcccatgt cgatccatca tataggctgc taataacgcg
     9481 attccccacg ctccaccttc tccggctgtg tccatcactg taactggtgc ctccatagca
     9541 gatgctagaa tccgctgtcc aacttctggt gttttaaaaa tccccccatg ccccactaaa
     9601 cgatcaattt ggacattctc agatttcaat atatccattc cgattctcat tgcaccaaaa
     9661 gcactggcta aatggatccg catgaaattc gctaaatcaa aacggctacc tggtgtacgt
     9721 acaaataatg gccttccttc atttactcct gtaatgtttt caccggaaaa ataaccataa
     9781 gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt tgttgaataa taccgtgaat
     9841 aatttatctc tgcttatctc catacccaaa gaattggtaa attcttcaaa aatactgacc
     9901 caagcattga tatccgaaga gcagttattc gtatgaacca tgcctactag ttctcctgaa
     9961 ggtgtagtga ccatgtcgat ttccggatgg acattttcta gttctttttc taatacgatc
    10021 atagcgaagg cggaagtacc agccgaaatg ttccctgtcc ctttggctac actgttcgta
    10081 gcgaccatac cggttcctgc atccccttct ggcggacaaa ccgggattcc tggtctcaaa
    10141 tttcccgtag ggtcgatcag ataagcccct gattcgctta acttgccagc ttcttctcct
    10201 gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg ctacgtgcgc tttttcgatt
    10261 aactgaccaa aaatattttt catttcttga tggtaggttt tcgttgaagg atcgatagga
    10321 aacatgcctg atgcatctcc tacgcctagt actttctcac cagtaagctg ccaatggaca
    10381 taacctgcaa gcgttgtcaa aaaattaatt tctttcacat gttcttcttt attcaaaata
    10441 gattgataca aatgagcgat actccatctc tgcggaatat tgtattgaaa tttttccgtt
    10501 aatattcgtt ctgcttcttc agtgattgta tttcgccaag tacgaaatgg tactagtaac
    10561 tgttccttgc tatcaaatgc tagataaccg tgcatcattg cactgaaccc aatcgacccg
    10621 attgtcgtaa gattctcgcc atagatttcg aatacttctg ctgccatttt tcggtaactg
    10681 acgcgcagtc ctttccaaat atcttctaat gaataggtcc agataccatt ttctagtttg
    10741 ttttcccact cataactacc agcggcaata ggagtaaaag atgaatcaat caatacagct
    10801 ttgatcctag ttgatccaaa ctcaatccct aaagatgtac gaccatctat gacatctaac
    10861 atactttgcg catttacttc cttcataatg atttaactcc ctccttctcg tcaaacctga
    10921 tcaaaccgtc tttttagtag cgcttacaat gattattgta tacttttgta cgtacatgtc
    10981 aagcagttat ttacaagaat acaataaaaa acacttgttt tgtacgtaca taagttataa
    11041 aatgaaattg cttacaaaat ctgatcaacc gccttctcga aattctagaa ggagagaata
    11101 cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt atttttttgg cgcatttggc
    11161 ggcattctat ttggatatga tattggtgtt atgaccggcg ccttaccttt tttacagcat
    11221 gattggggtt tggcaggtaa agcttctttg ataggatgga tcacgtcttc ggtcatgtta
    11281 ggtgcgatcc ttggcggctc tttatctggt cttctttctg ataagcttgg acgacgaaaa
    11341 atgattcttc tttctgcact tatttttatg gctggttctg tcctttctgc atctgcccct
    11401 cacaatggtt cttattttct gattgcagca aggattcttc taggtttagc tgttggcgct
    11461 gcatctgccc tagttcctgc ttatatgtct gaaatggctc ctgctagact aaggggaaga
    11521 ttgtcaggga tcaatcaagt gatgattgct tctggcatgc tcctttccta tgttgcagat
    11581 tatttgctaa aagggttgcc tgaaacgatg gcttggcgcg tgatgttagg gctcgctgct
    11641 gtccccgcgc tgattttatt tttcggtgtt ttagctctac cagagtcacc gcgttttctt
    11701 atgcaatctg gccgcttgga agaggcaaaa agagtattga actatattcg gacaccaaaa
    11761 gaagctgaac aggaattcga acaaatccag ctgaatgtca aacaagaaaa gacaaccgga
    11821 acttcttggc atactttgtt tttagaaaaa tatcgttcct tagtcattgc cggtattggt
    11881 gtagcagtat tccagcaatt tcaaggagct aatgctattt tttactatat tccgctgatc
    11941 gtagaaaaag cgactgggca tgcagcgagc gatgcattga tgtggccgat catccaagga
    12001 atcattttag ttgctggttc tttgctcttt ttggttattg cagacaagtt caatcggcga
    12061 actttattga aaataggcgg tagcgtgatg ggactttctt tcatcctacc agctgtttta
    12121 ggaactgtac tggatgctca tacaaattcg ttgttgattc tcttgttttt atgtatttat
    12181 gtcgcatttt attcttgcac atgggctcca ctgacatggg taatcgtagg cgaaatattc
    12241 ccactagctg tccgcgggcg agcatctggc ctagcctctt cttttaattg gattggttct
    12301 ttcctagttg gcttgctttt tcctgtgatg actgcttcta tgtctcaaga aatcgttttc
    12361 ggcatttttg ggatcatctg ctttttaggg gttttattta ttcaagagat cgtaccagaa
    12421 actcgtggga aatctttaga ggaaatcgaa caaagtgcat ctaaaaaaac ttatcctaaa
    12481 cgtatttcta tccatcattc ttaaagaggt tgtgacagaa gtggtcagct tcaagaaata
    12541 agaaggaacc gcttagtttc cagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    12601 tgatgaatat tgtgacttgt gaccagttgg taggagaaca ccgtttagtg gagaggtggt
    12661 gaaagaaacg ttcagccttg agcgatgaca aaagctctca caaacattgc gacgagtaac
    12721 cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt tttcgttttc tttcactgtt
    12781 tcccgttcaa ctagtacagg ttcatagaga atatttggct gtgcctgtcc tttttcaatc
    12841 gttgtgacga tccaccgtgc ggcatcttgt cccaactttt cttttggatg cgtcaatgtt
    12901 gtcaattttt tattccctaa atggcttaaa gaagaatcgt ctgttcctac gatcgatagg
    12961 tcatctggaa tatgatagcc attttctgtc aacttatcta gcattaaact agctatttga
    13021 tcgttatagc aaatgatgcc atctaaagct tgttcttctt tcaaccgttg tactgttttt
    13081 tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga tatcttccgt caaaaaacgc
    13141 acattatact gttcacacgc gctgataaaa cctttcatcc tgtattttcc ttgaagatcg
    13201 tcgatttttg tgatgaacaa cagacgctga tgtccttttt ttatcaaata ttctgctgcc
    13261 ataaaaccga ctttcacatc atctaaacaa atataagagg catttaactc ttcataagca
    13321 gcattgatca tcaccatcgg aatgccttgt tctctcaaag caacataaag tgctaagttt
    13381 gggttatact gattactttt cgtaggctca acgatcaaac catctacccc ataagcaatc
    13441 attttcttca aacactcttt ctccaattcg tgatcgttat tcgtactggc taaaagcaat
    13501 gaatacccag attctcgtaa ttcattttcg attcctcgga taatcgacgg aaaaatataa
    13561 tcagagagat atgtggtgat gacaccgatc gtttttgttc tgttcaatgg tgttttttct
    13621 ttcttatagc tgtcatctac atatgtgccc gacccttttt ctttacgcag atagccctca
    13681 tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc tgacgtcata tatttcttgc
    13741 agcttgaatt ctgagggaat ggttgtattt acttcgtatt cctcacgaag gatcttttgt
    13801 tttatctctt ctgcaatatc ttgatatttt gccatttgcc ttgccccttt ttatttgtac
    13861 gtacatatat cataacgagt ctgcaaggtt tattcaagca aaaatcaagg tttcttgtgc
    13921 tcataaggca tacggaacag gttcaccaaa ttctggaaat ccttgctcat tccacgtaaa
    13981 gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga tccccttttt ggttcttctc
    14041 tggtcgagca tgatagatca aaatatcaaa gcacccgtct tctgaaaccg taaacgagtt
    14101 gtgaccagga ccaaattgct tatttttttc agaagaaacg aatactggct ccgataattt
    14161 atgccatgat tcttttaata aaagattctt gtgctcatca gcccacagca ggcccatggc
    14221 atagttttca tctgttgcac taccagaata cgtaatgaat attttcccat tgcgagtaag
    14281 gactgctgga ccttcattta ctgaaaaacc ccgtttttcc cattcatact caggtataga
    14341 aaggaggatc tgcttcccct ttaacgtcca aggattatcc atttcggata gataaagatt
    14401 agaattccca gtaatttcag gatcttgttg ggcccacaca taatatagct ttccttgaga
    14461 agagaaagtt gttccgtcta aactgaaagt ttcccttttc gtttttattt gtcccttttc
    14521 tatccaatct gtattcattg gatctatatg ctgattttca agtacgaaca tgcggtgatg
    14581 atgagattca tcacgtatag ttttatgatt gcttgcagca aagtaaatat accattttcc
    14641 attgatataa tggacttcag gtgcccagat caattcactc atcggtcctt cttcatgagc
    14701 ctgccataca atcaggcttg cagcatgttc taattcattc agtgactttg cccttcttaa
    14761 ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa taataaccat ctgtatgttt
    14821 atatacccaa ggatctgccc gttgccgaac gataggatta ttataaagtg tcttcatcat
    14881 aacaaatttc ctcacatttt ctttattcta attacattcc aagagtattt ttctatccct
    14941 acatttaatt cgtcatgttt ttctattgaa aaatagttta tttcaggttt tattttttct
    15001 ggttcttcac gtgtattttt gtccaacaga tcgctactgg ttaatgtgta gtgctctcct
    15061 tgatctagca aattgaaatc atgttcaatc gttatctttt gttgttcatt tgtgcgattg
    15121 atcaagaaaa tagtcagctg attgccgtta tctacgatca cactgtccac ataaggaaca
    15181 ttttttatct tttcacaatc ataaacaggg caaatgattt gttgtttcaa tacttgacct
    15241 ttagcatatt tcgataagtg ttgcatcaca taataagttg gattcctcca tgcttgtcct
    15301 ttagcatctg tcagtattaa aggtattgtg tttactaaca atgattgaca actaatcttt
    15361 acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac caaataataa tgtatcttcc
    15421 atagtaaagt aacaccaatc gatgggacta ccgacttcaa aatccctatg tcttttttcc
    15481 ggttgggcat gtacattcca ttcatcaaac gctagataca ttgttttctc acttctcaaa
    15541 tatcctttta cataatcaca agtagcaacg atttcttcaa tttgacgatc gaatcgataa
    15601 gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt taggaggttt agtcaagtct
    15661 tcttcatcat acttatcaat atagttatgc aaagctaaat aatctacatt ctcataagct
    15721 tcttctaatg cgatacgatc ccattcaggg tacgatggta aacgtggcgt agagcttcca
    15781 actaagattg tttcaattga atcatccact aacttcatga cttttgaggc ttcatttgct
    15841 aacctaccat attcataagc actcttagcc cccacttgcc atggaccgtc tagctcattc
    15901 cctaaacacc ataatttaac gccaaaaggc tttttttgcc cattttttat tcttaaatca
    15961 ctccagaagg ttccttttgg aaaattacaa tattctacta aattttttgc ttcatcaatt
    16021 ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt gtacatgact gatccattta
    16081 aaaaattcgt ggagtccaaa agaatttggt tctatacctc gccatgctaa atcaattttt
    16141 tttggtcgtt gttctttcct acctacagta tccacccaat cataaccaga tgtaaagttt
    16201 cctccaggat agcgaatgac acctaattct aattccttta cataagagag gacatcttgt
    16261 ctaaacccgt caccatcagc tgtttcatgg cccggttcat aaatccctgt atagacgaca
    16321 cttcccatat gctcaacaaa tgagccaaac agccgagaac tgatctctcc taccgtaaaa
    16381 tcctctctga tttttacttt cacttcttcg tccaacagct ttccctacct ttcatttcct
    16441 atagtcaaga taatagatac aagtttccat acgccaccag caaagataaa aataaaagga
    16501 gggaaaagaa atatgagcag tacaaatcct aataacaata cgcaacaaag aatatttctc
    16561 aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa tttgtaaaaa tgtgatatct
    16621 ttgttttcag aaatcacata tatccaggat aaaagaaaca tggaataaat aagtaaaact
    16681 acatatccac taattgaaag tacgattgtc tgttcttcta ataattgatt aacaagaaaa
    16741 taaataatga tcgttaacag actaaggaac aagttcttac caaaatgttg ctttattcct
    16801 tgaaagaaaa aataaatgat atttcctgat ccctcacctt tccattgcgc cattacataa
    16861 aaaacagcac atgtagcagg cacgatagta ataacaggca aacacacgat tacccagaga
    16921 atacctaata agatgtaatc agaaaccaca atcaactgtc cataaagttt agtctcaact
    16981 attttcatct tcctcactct ttcattccag acatagctgt tccttgaaca aaatatttct
    17041 gtgcaaaaat ataaaccact agtaatggca gcattgaaat aaccgttcca gccattacag
    17101 gtccataaaa agaaatgttc tgccctgtaa ataaagctaa ccctgctggt aaagttctca
    17161 tttttgaact acttgtcaat atcaatggat aaagcaagtc attccaacta ttcatcagcg
    17221 tgaaaatccc taatgataaa agagtaggtt tgctcaatgg caacatgatt ttcaaaaaaa
    17281 tattaaactc acttaatcca tcaatgcgtg ctgcctcttc caaagaatct ggtaaagtaa
    17341 taaaaaagga acgcatcatg aagataccaa atgcagttgt catacgagga atgatcaaac
    17401 ctgcataagt atcaagcatg ccaagcaaat tgacttcaat aaatagagga atcatgaaga
    17461 cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa caaaactgat tttcctttga
    17521 aacgcatacg agcaaatgca taccccgcaa gagaatcaaa gaatacagaa gtgatcactg
    17581 tccctccagc aaaaatcact gtatttttga tgtaatctaa taaagggatc gttgtccaaa
    17641 ccttaatata gttatctagg gtatagctat ctgcaaatat tctaggcgga aatgagataa
    17701 tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa aaaaggaaac gcaataacca
    17761 acaatataac tagcatacta atcgttcgaa tgattttttt accactaact gcttttcgac
    17821 tattcacagt aactgtaatc gagctttctg gtgccataca ggtttccctc ctatttcatt
    17881 tgtttttctt tccgtgacat gtacacatta gtggagatag ctatagttgc aacaatgata
    17941 aacaaaaccg aagacagtgc tgatgcatag cccaattcat aaggtgctgt aaatcctcga
    18001 ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg ggccaccagc ggttgcaaca
    18061 tatacttggt caaacacttg catcgaacca atcagattcg ttaaaataca aaaacctaag
    18121 gagggaacga tctgaggtag cgtaatgtta aaaaactgct gtcgatttgt tgcaccatcc
    18181 atttgagcag attcatacaa agagggagag attccctgaa tagcagtcaa gagaatcgtc
    18241 atagtaacac caaaattctt ccaaacagtc atgatggcta ccgtcggcat agcaagggtt
    18301 ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg aaaacaagta aggaatcgcc
    18361 cctacattcg gatcaagcaa catagaaaaa ataatcgcaa ttgacgtcaa tgaacaaata
    18421 actggaatgt aaaatgtcga acgacaaaaa cgattgaatc tggtattttt agataatgct
    18481 actgctgcta ataaaccaac gataatttgt gttggcgttt cgagtacggt gaaatataaa
    18541 gaatgaatca acgaattaac tgctcgttca tcactaaata tacgtgtgaa attttcaaaa
    18601 cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg taaaactaat agcaaatgtt
    18661 cctaaaagcg gtatgacagt aaataataat aaaactaaaa aagatggagt taaaaacaaa
    18721 tacgcagcac gatcaggacg attccaatat ttggtgaatc cgttcatata gtccacctca
    18781 tctttcattt tgataaaata tacaatcttg gtttaaacga atctgaataa gatatccgaa
    18841 caatcgctgt gatttttgca ataacaccta tctgcaaata ctaggtcgat cattcagata
    18901 tctgtatctt gccaacagtc tatcctgtat aaagttctgt actatttttg cagtaattga
    18961 ttgatggcat catctgcatc gttcatgagt gtctcaggat cttctcctgc taataatttt
    19021 tcaatcagtg gattgatgat atcgttattg atcgcagaga tttgggtaaa acctggtaaa
    19081 tatggctctg catagtccat ttgtttcgat aattcagata caataggatc attttttact
    19141 tcttcatctt ttgctactga atccaagtag gcaggaaaac cattttcgat actccatttt
    19201 ttaccaattt cagttgtatt ccaatatttc aaaaactcat agacagcttc ttttttatta
    19261 ttatctgtac ttgccggaat ggcatatcct acgccatcta aaatagctgt ttttttgtct
    19321 gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa tctcgtttgc tttcaatccg
    19381 ttatttaacc atggcccatt gatttcaatg gctagttgac cagcattcat taaattgtcc
    19441 atttcggcgc cagaaatcga ttctggacca gtcttgtcta catggatcat tttttggatc
    19501 gcttttaacg ttttaaggtt ctcaggagac gcaaattcag acttagtata atcatcgttc
    19561 actaatttac cgccatttgc taacatccag ttatataacg gtgcagttcc atccttattg
    19621 aagacaaaac ctgatacatt tttactacta tcagttaatt ttggtgccat ttctgtcaat
    19681 tcttcccaag tagtaggtgg ttgttcagta tctaatccag cttgtttaaa caaatcatta
    19741 ttccaataca tatacatgcc ttgtacctgc atggggacaa aatactgttt accttctact
    19801 tcacctaatt caatagccgt ttctttaaaa tcagatttat ctactccatc atattcccag
    19861 aaatcgtcta atggttgtac cgctccgttt tctacatact gtgcaaaatc tggataattt
    19921 aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg taggaagttt ttcattcaaa
    19981 ttggcccaag tcatgatatc catttcaatt ttgatgtttt tatcatttgt tttattaaat
    20041 tcattaacga tttcttgcaa tatttcacca tcagatgcag taaacccatt ccaaaaagtc
    20101 aaagtcactt ctttatcaga atcagattct gctgaacttc cgccaccgca agctcctaaa
    20161 atacctgttg cagctaccaa caaagagcca attaaccatt ttttaccttt cattttttgt
    20221 tcctccttca tctgataaac gacatctgac cacattccaa gatagcggtt tcaataaaca
    20281 attgattttc ccatcttcag cttttacatt tgttgaatga cttggtttta cttgttcact
    20341 tttaggaaga ttagttgctt ttgtatcaaa tccactcatt tcagaaaaat caatcactcc
    20401 ctctaatgat aattcagaca agtgaacagt tagattcatt tcttcttctc cacggttaac
    20461 tgcaaatatg acaagttctt tgtcatttat tacggctata ctttctaaat acggcacttc
    20521 ttcaaaatct tccgtcgcat agtttgctga acgaatttgt gggcttaaca cctctccttg
    20581 tccatacaca gcagcttgca taaatggata atagatcgtc tgtctccaag cttctccgtt
    20641 tgtttctgtc ctgattggtg caataacatt taccaattga gcaagacaag caatctttac
    20701 tctatctgca tgtttcaata aagtgattaa tagacagcct aatagtaatg catcttcaaa
    20761 attatagtta tcttctagta aaggaggagc aatctgccaa ggcgctgctt gctcatcttg
    20821 ttccgctgaa tgataccaaa tattccattc atcaaaggac aggttgatct gcttcttact
    20881 atgtttcttc gccttgactg tatcgcacat cgctgtcact tcagaaatga accgatccat
    20941 gtctaaggaa cgtgcaagat aatgagaaag atcatttttt tggttgccat aatattgatg
    21001 cagagacaaa taatctattt gatcgtacgc ttgatcaaga actgttaact cccaatcacc
    21061 aaatgtaggc atttgccgat tcgaacttcc gcataaaacc acctcaattg aatcatctac
    21121 gagtttcatc gccttagccg tttcctctgc taaccgtcca tattcttccg ctgttttatg
    21181 tccaatctgc caaggaccgt ccatttcatt ccctaaacac catgtcttaa ttccaaaagg
    21241 gctctcttga ccattcgaac gccttaaatc actccaataa gttcctttag gaaaattaca
    21301 atattcgact aaattacgag ctgcatctac tccacgagta cccagattga cagccatatt
    21361 gatttcggcc cccacatgct gacaccagtt ataaaattca tgaatcccaa cttcattcgt
    21421 ttcaattgtc cgccaagcta aatccaacct tctaggtcta tttgctatgg gcccaatact
    21481 gtcttcccaa cgaaatcctg acacaaaatt tccaccagga tatctaatca tgggaatgtt
    21541 caattctttt accaagtgaa tgacgtcttg gcgaaatcca cttttattag ctgatggatg
    21601 atctggctga taaatccctt catatactgc tcgtcccata tgttcaataa atgaactgta
    21661 caaccgcgga tcaactttgc ctacacgctt gacactacta atcaacattg tggcttccat
    21721 aactgacctc ctttaattta tacatgaata atacacctcg ttgaaagcgt tgtcaataat
    21781 ataaatgtta aacttactta ttttttatcg attttaatta accaaaatat taataatgat
    21841 taaaacaaat aatttaatat aacttttatt aacaaatatg ttaatatata agaaaaacct
    21901 tttaagaggt gatatatatg caactacaaa taaacgaaga atccttaccg gtatatgaag
    21961 cattagctag taaaacaagg atcaagatca tccagctact ttccaaaaaa aagatgaatg
    22021 tcaaagattt ggcaaaagaa cttggagtga gcagtgctat cacgacgatg cacgtgaaaa
    22081 agctagagga agcaaacatc atcaaaacag aaaaagtagg acaacaaaaa atttcgagcc
    22141 ttcgtgtcga taaaatcgat atcagttttc cagaaaaaat attcaatgct tttgatacga
    22201 aagaaacatc gatacctatt ggtcactata caaattatgc aattgaacca acatgcggtt
    22261 tagctacgat ccatgatttt atcgggaaag tggatgaacc acgctatttt atggatcctc
    22321 gaaggatgga tgcccgcatt ctctggttta ccagcggatt tgtcgagtat caggcaccta
    22381 attttttaaa tacagaggat acattggaga tgttggaagt ttctgtggaa atttcttctg
    22441 aatttccttt ttcaaatgac aattggcctt cagatatcac tttcagccta aatggtgtgg
    22501 agctaggaac atggacgagc cctggagatt ttgctgatat ccgcggtaaa tatacgcctg
    22561 attggtatcc agataatctt aatcaatacg gcttgctgaa gacgatccga attaccaaac
    22621 acttgacgaa tatgaatgga gaacccttgt caaacatcac gatcaacgac atccctaaag
    22681 agcaagacac ttggcatttg cgaatcgaag tgaaagacga tgccaaacat gtggggggct
    22741 gtactttatt tggtaaagga ttcggtaatt acgatcagga tatcaaatta aaagtttact
    22801 acagctgata tttttctttt ctattttata aaacaaaaac agccaatccc tatgaaggga
    22861 cggctgtttt tgctttattg ttatttccag aaatcatcca aaacggaaat tggcaaatgt
    22921 cgtttatgtt gtgtcttgtt ccaccatgct tcgatttttt cttgtgcttg agcgggaact
    22981 tcttttcctt ctagataatc atcaatttca ttatatgtga cacctagtgc gacttcatct
    23041 gcaatcattg gtttcccatc ttcaagatct gcagtcggaa tttttgtgta taatttttcc
    23101 ggtgcattca atgcttgaag caattgtttc ccttgtcgtt tgtccaaacg gaataatggc
    23161 aaaatgtcgg ctccaccatc accaaatttc gtgaagaatc cagtaatgtt ttctgcagca
    23221 tgatctgtcc ctaatacggc tcctgctctt tctccagcta cggcatattg cgtaatcata
    23281 cgttgacgtg ctttgatatt tcctttgtta aaatcgctga ttcccacacc tgcttcgctt
    23341 aatacttgta cttgtgcatc aacagctggt ttgatattta ctcgcagact aacatcgggc
    23401 tggataaagt caagagcagc ttttgcatct tcttcatctg cttgttcacc ataaggaaga
    23461 cgaacagcga taaattgata gttttcatct tttgtttctt cacgcatttc ttccatcgtt
    23521 agttgcgcca aacggcctgc taaagtagaa tcttggccgc cgctgatacc tagtacaaaa
    23581 gttttaagaa acggatattt tttcaaatat gctttcataa agtcaatact tttacgtatt
    23641 tcttcttttg gatcaattgt tggtttgaca cctaattcat tgataatttc tgtctgcagg
    23701 ctcatttatt ctcctcctat gcttctgtat gaagttctgc tacgcttttt ttcattcgtt
    23761 ccatgatagc cattttatgg ttccagcatt cagtagacag atcgactggg tatttttgcg
    23821 gattcaagtc tcgtttatac tcttcccata gtgaatctag attttctttt gtatattctt
    23881 tgatttcgtt tagtgcaggt aattcgtaaa ctctttttcc ttcgataaag atatcttgta
    23941 aaactgggcg agctgtaaag tctcgaacag ttttattgat aaacgtatgc actggatgga
    24001 acatgaagat agcttcttct tctcgagggt cttcatccca taatgtcacg tagtctcctt
    24061 cagatttccc atcaaaattt cgagtgatgc gccacacttg ttttttccct ggagtagtta
    24121 ctttttctgc attgcttgat aacttgatcg tatccatcat ttttccttca tcatcttcga
    24181 tcgatactag cttgaatact gcaccaaggg ctggctgatc ataggctgtg atcaattttg
    24241 tcccaacacc ccatacatca attttagctt tttgcatctt taaattcaaa atcgttgctt
    24301 catccaagtc attcgaagca tagatttttg cttccgtaaa tccggctgca tctaattgtt
    24361 cccgaacacg cttagatata taggccatat ctccactgtc gatgcgtacg ccttggaagt
    24421 tgattttgtc acctaattct ttcgccacgc gaatcgcact tggcacacct gatttcaacg
    24481 tatcataagt gtcgactaag aacacgcagt ctttatgggt tttagcgtag gccataaatg
    24541 cttcataatc atttccataa gattggacta acgaatgagc atgggtcccg ctagctggta
    24601 tcccaaatat cttcccagca cgtacattgc ttgttgcatc tgctccgccg atatatgccg
    24661 ctctagttcc ccagactgct gcatccagtt cttgtgcccg tctagttcca aattccaata
    24721 gtggatcttc tccgatgaca gatttgatac gagctgcttt tgtcgcgatc aacgtttgga
    24781 aattgaccat attcaacagt gctgtttcta ctaattggca gtgcgctaaa ggcccttcca
    24841 cttggattaa tggttcattt gcaaaaacta gttctccttc acgagcagaa cgaactgttg
    24901 ctttaaattc aaaattttgt aaatactcca aaaaaccttc aggatagact tctaatccac
    24961 gcaagtaagc aatatcggaa tcattgaacg tcagattctc taaataattg actaaccgtt
    25021 ccaatcctgc aaaaaccgcg tatccgtggt taaaaggcat ttctcgaaag taacattcaa
    25081 aaacagcatg taaatcagct cgacctaatt cccagtatgt ctgcatcata ttgatttgat
    25141 atagatccgt atgcagcgtc aaactgtcgt ctgggtaagt attattcatc ttgtattact
    25201 cctcttggca agttttcatt tcctacccat tataacaaaa aaaattaata ggaaaagaaa
    25261 tcttccattt tttctgaaaa agcacatgtt ttacggatat ttcttgctaa aaatgaactc
    25321 catctgctaa aactatcctt tcttattggt atttaaccta aacgagaaag aatacttatt
    25381 ttattcaatg tatccgagac acgaagaaat tagcgttttc tatttttctt acttgataaa
    25441 ataagtagat tttcctacta caatcgtagc cgttaagata ccgatgattg aacaaatata
    25501 accaaatgtc tctacaacat aatcaatggc aaaagttttt gaagcaataa tacgtctttc
    25561 cttttttgct tcttatcccc aaagaaacgg atataactta ttatgtattg aatttttttg
    25621 gaaaggcgaa ttatgacaag atataccttg cgttgaaaaa actcgcggaa aaaatatcac
    25681 acgtttttaa aaagatacaa agtaagaaag cagctgtcaa tagaatggaa acaaaaatgg
    25741 cgagacatca atcagtctcg ccttgttttt tattgtatac attagatatg gagcaggtta
    25801 ccataaagat gattattagt cgttattaaa tagaatcctt cgtatttttt tgttctatca
    25861 ctacaatatc tttatttgga cgtttgaatt gtattccggc acgttctagc tcctgtttca
    25921 ctcctacatg gactttgaca tcttctcgtg tcatttttaa aacgtgatga atcgcatagt
    25981 cgatttcttg ccttgtgatt tttttcttcg tattgatcat cacgacttgc tttttatcat
    26041 atgtgtctac caatagcagt tgtgttcgat ctaacgaaaa taatttgtaa taattaatag
    26101 ccgtattccc aaaaaagaat tccactactt taggataaaa tgtcttaata tttaaattac
    26161 tattcacagg tgtttcgatt agcttcatct cattcctctt ttctatatag tcgatttaga
    26221 tccaatctta agttatgtta ccatctttcc tagcattaag gaatcggtta caattgaacc
    26281 tttaaagaca aaaggattta ccttttatca cttaatagta taacataatt tcgtgagaaa
    26341 aaaatacaat tttttcttga ccatctcgtt acgtaaagga ttatgcttac atttgtcagg
    26401 aggaaaaata atggagtaca caataaagaa aatgtcagag atatcaggag tcagtccgag
    26461 aaccttgcgc ttttatgatg agattggact gctaaaacca gctaggatca attcttcagg
    26521 ttatcggatt tatggcaaaa aagaagtcga tcgtctgcaa cacatcttat tttatcgaac
    26581 aatggcattc aagttggatg atatccaaga ggtcttagac aatccatctt ttgaccacca
    26641 gaaagcttta atcaagcatc agcaaatgtt gctggaaaaa cgggcacaaa tcgataccct
    26701 cttgacaact gtccaacaga cacttgatat gtacgaagga ggaagaaaaa tgagtgacaa
    26761 agaaaaattt gaagggttta aacaacaaaa gttgaaagaa aatgaagaat cctatggaca
    26821 agagattcga caaaaatacg gaaaaaaaaa ggttacagca tccaatgaaa aatggatgaa
    26881 tatggataaa gaaacgtatg aagaaatgca aactgttgaa aagcagatgt tatttgatct
    26941 cactctttac ttaaaagaac cagaagatga aagcttagca gaccgtatct tccaagctca
    27001 taagaagtgg ttctcttaca gctggcctga atatcagcca aaagcgcata aaggactagg
    27061 aatgatgtac gaagccgatg aacggttcac tgcatattat gacgagcgaa gtggtcatgg
    27121 tgcagcaaaa gctttaaatg aaattatcca aaaaaaggcg taatctttta catgatccaa
    27181 caaaaaagta cacgtaaaat gacctaataa caaaacagca gacacccaag ttagattttt
    27241 atgctaactt ttggtgtctg ctccattctt tcacaagttt tttcatccta ctttaaatat
    27301 attcatcgtt gaataaacgt ataaggtatt ttcactttat cctttttttc tgtaagaaac
    27361 aattgaaatg ccatttctcc gcaagcttcc agatggtggt cgatggttgg gaaaccaaga
    27421 agctcgctga tcagcaaatt atcgcgtcca atcaccactg gtgtcttatc ttgcagatag
    27481 ctttgcaaga tgcctgctgc aaccatatcc gaatttgtaa agacaccatc aactccaagc
    27541 tctttaaaaa agaagcctgc ttgttttcct ttctctgcat ctatacaatc ccaaaaaaca
    27601 agtgattcat caaaagatgg aaaaagttga tgacatagct gcaaagtgat ttttgagtta
    27661 taacttagcc tgccacttcg ccccaacgtg atgcccaatt gctttatacc ttgctgcttc
    27721 aaataagtaa gaccttctgt aattgattgt tctcgatcaa taaagacaca gcttgcctgt
    27781 tttcccttga tttcttcaca aaaaactaga ggtccatatt tttgatatgc caataaatca
    27841 tctagtgtat tggcacgaga cgtaatgatc aatccatcga aagctttagc tgcaaattct
    27901 tcaagatacc ttcgctcttt ttctttatga tagcccgtcg gtaaaagtgt cactttgtaa
    27961 tcataatcaa acgctgcttc aatgatccca ctgatcaact gatcaaaata cggatgattg
    28021 gtataaggca gaatcactcc aatgcttttc gtttttccat aacttaactg tctggcattt
    28081 gcattaggga tataatcgac ttctttcatg accgcaagta tttctttcct tttttcctca
    28141 tctacatatg gatgtccatt gattacacgt gaaatagtag aaactgaata acctgtgagc
    28201 tttgcgatat ctcgaatgtt tgccactgct gcttcgccac ctatacaaaa agattttctt
    28261 gtaaatagtc taccacagct ggaccgcatt caaaaaactg atacattttt ccttcatgcg
    28321 aaaaactgat ccgctgcccg ccccaatata tatcttttgt caaattttcg atttcattca
    28381 ttggtatttc ttttactata ttcctattca acccttgaaa actgtacaac gttaatacgg
    28441 atcccttgaa ctcgacaaac gcataaaaat aattgttcgt tgtgccatct gttatctcta
    28501 cataagtatt cattgcaagc cctccttttg atactttgag tatagtgtat gaaacgcgtc
    28561 tcatagcaag gagtttgttt tgccaaagaa aagttttatt cctctttcat tattaccaaa
    28621 gtctcttatt ttaaattgaa tacattataa tgatactgta aatatatctt tattattagt
    28681 gaat
//
LOCUS       DAFFFQ010000027        28615 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000027
VERSION     DAFFFQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.027"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(321..1118)
                     /locus_tag="LQB69_001303"
     CDS             complement(321..1118)
                     /locus_tag="LQB69_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL3525478.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1115..2299)
                     /locus_tag="LQB69_001304"
     CDS             complement(1115..2299)
                     /locus_tag="LQB69_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL3525479.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2324..3571)
                     /locus_tag="LQB69_001305"
     CDS             complement(2324..3571)
                     /locus_tag="LQB69_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL3525480.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3786..5531
                     /gene="dnaX"
                     /locus_tag="LQB69_001306"
     CDS             3786..5531
                     /gene="dnaX"
                     /locus_tag="LQB69_001306"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL3525481.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5551..5865
                     /locus_tag="LQB69_001307"
     CDS             5551..5865
                     /locus_tag="LQB69_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL3525482.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5890..6486
                     /gene="recR"
                     /locus_tag="LQB69_001308"
     CDS             5890..6486
                     /gene="recR"
                     /locus_tag="LQB69_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL3525483.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6550..7194
                     /locus_tag="LQB69_001309"
     CDS             6550..7194
                     /locus_tag="LQB69_001309"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL3525484.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7209..7538
                     /locus_tag="LQB69_001310"
     CDS             7209..7538
                     /locus_tag="LQB69_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL3525485.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7552..8490
                     /gene="holB"
                     /locus_tag="LQB69_001311"
     CDS             7552..8490
                     /gene="holB"
                     /locus_tag="LQB69_001311"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL3525486.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8526..9350
                     /locus_tag="LQB69_001312"
     CDS             8526..9350
                     /locus_tag="LQB69_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL3525487.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9343..9690
                     /locus_tag="LQB69_001313"
     CDS             9343..9690
                     /locus_tag="LQB69_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL3525488.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9759..10631
                     /gene="rsmI"
                     /locus_tag="LQB69_001314"
     CDS             9759..10631
                     /gene="rsmI"
                     /locus_tag="LQB69_001314"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL3525489.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10740..11861
                     /gene="dinB"
                     /locus_tag="LQB69_001315"
     CDS             10740..11861
                     /gene="dinB"
                     /locus_tag="LQB69_001315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL3525490.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11915..12517)
                     /gene="nrdG"
                     /locus_tag="LQB69_001316"
     CDS             complement(11915..12517)
                     /gene="nrdG"
                     /locus_tag="LQB69_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL3525491.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12832..15021)
                     /gene="nrdD"
                     /locus_tag="LQB69_001317"
     CDS             complement(12832..15021)
                     /gene="nrdD"
                     /locus_tag="LQB69_001317"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL3525492.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15320..16114
                     /locus_tag="LQB69_001318"
     CDS             15320..16114
                     /locus_tag="LQB69_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525493.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16104..18146
                     /locus_tag="LQB69_001319"
     CDS             16104..18146
                     /locus_tag="LQB69_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525494.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18427..18576
                     /locus_tag="LQB69_001320"
     CDS             18427..18576
                     /locus_tag="LQB69_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL3525495.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18587..20101
                     /gene="dltA"
                     /locus_tag="LQB69_001321"
     CDS             18587..20101
                     /gene="dltA"
                     /locus_tag="LQB69_001321"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL3525496.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20098..21303
                     /gene="dltB"
                     /locus_tag="LQB69_001322"
     CDS             20098..21303
                     /gene="dltB"
                     /locus_tag="LQB69_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL3525497.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21348..21581
                     /gene="dltC"
                     /locus_tag="LQB69_001323"
     CDS             21348..21581
                     /gene="dltC"
                     /locus_tag="LQB69_001323"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL3525498.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21584..22852
                     /gene="dltD"
                     /locus_tag="LQB69_001324"
     CDS             21584..22852
                     /gene="dltD"
                     /locus_tag="LQB69_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL3525499.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22993..24075
                     /locus_tag="LQB69_001325"
     CDS             22993..24075
                     /locus_tag="LQB69_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL3525500.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24075..24878
                     /locus_tag="LQB69_001326"
     CDS             24075..24878
                     /locus_tag="LQB69_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL3525501.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24948..25688
                     /locus_tag="LQB69_001327"
     CDS             24948..25688
                     /locus_tag="LQB69_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3525502.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25699..26703
                     /locus_tag="LQB69_001328"
     CDS             25699..26703
                     /locus_tag="LQB69_001328"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL3525503.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26748..28592)
                     /locus_tag="LQB69_001329"
     CDS             complement(26748..28592)
                     /locus_tag="LQB69_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525504.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         9022 a         5059 c         5886 g         8648 t
ORIGIN      
        1 tttaccaaga ggttgtgagc ctgccgttta actctgagtc acaaggaaca atttcaaaaa
       61 acagcttttc atgtttgatg aatattgtga cttgtgaccg aggagttggt aggagaacac
      121 cgtttaccaa gaggttgtga aaaagctgtt ctgcatcaag taataagaag agccaagtaa
      181 aaacagtctc ttatgttttt aacttggctg gacttatcac cgtagatgta agcttttgaa
      241 cgccgtttat ttgcaacaaa aggggctgtg acacgatttt tgtcacagcc ctttttttgc
      301 gaacattcat ttagcaattt ttagaaactt ctgccgccac cgccaaaagt cccgccaccg
      361 ctagaatggg tagtacttcc gccaccgccg cttccaggag gtgggctttt ggggattctt
      421 cttgtcgtga caaaagaatt tgtcagacga tcggtttttt cagttaattt gatactggat
      481 ttttctcgga atggatattt gtagatgcct gatttcaatt gataacgcga aacagtcaca
      541 aaataaaaga caagactaag gataagagcc aaaacaacag caatgatgat ttctactgtc
      601 gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt ctacacgata atgtcctcct
      661 ggaactccgt cttccacata tgttgctgat ttagacaggt aagcttttgc agcattgtaa
      721 taggcagcat ttgtcatctg atcataaaca tcatctaaga tcgaatcgat tctgctatca
      781 gtcaaataat cgatcatatt accagaagtc gaaatgtaga tttcacgctg tcccatatct
      841 aatagcaata ctgagccatt ttgatcattt ccgactgcgt ctcttaagta attatcagca
      901 aagtctcttg gttcttctga attagatgta gtcgttacga tcaagactcg tcctttgatt
      961 ttttcattca gcttttctgc ctgttgttcc aaagattgga tttgatctgt tgtgaataac
     1021 ccagcctcat catttaccgt agctgtatct gcatgagtag ccaccggcca gccaacactt
     1081 actagcaaaa gaaagaataa aaaaaatttt ttcatcatat caagtaacct ccgatcagaa
     1141 agaggatcgc tagcccagcg aaaatcccaa gagcggtcag tcccaatttt ttctggctga
     1201 tcggcaggac accgctgacc tttcctgttt gtccattcat tgcgtagtag tagacttttt
     1261 tacttgagtc attgcttcga taagtaacaa gccataccgg cagtaatacg taattattta
     1321 cttcgtttgt gattgaagca gaagttcgca caccggtcaa tgttgtatat ccatttgcgg
     1381 tatcttttaa cagcttctct gaatagtctt ttaattcaga ttgaatctgt gatttgattg
     1441 cctcatattc gatatctctt ttttctgctt ggaacccggc taaatactgg cttttaaaat
     1501 caattgcttt atctaacggg aaaggttgca cgccttcgac catcttttgt tgcgtatttt
     1561 ttgataaggc attttttact agttctttaa aagaaagctt gcctgctctt gaaacagcaa
     1621 attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca tatgcggata actgtaccat
     1681 tcgcctgcat ggcaccatct acttcagcat ctgtagccca ataagggaaa tacacacctg
     1741 tcaacttatc gatttgatcc ttattgaaaa aagctttggg aacaaaccat tttttcttgg
     1801 tccaagcgag aaatctgttg attgcttctt ctttctcgac agcaaaagga agtactttat
     1861 ttggcaagaa ttctccgctt aaacgtcccg atagtacgac gggattatgg cagtagtaac
     1921 aataagtcgc agcagttgtc gcatcagtca cgatctctgc accacagttg ggacaaagaa
     1981 aaagatctat cgctgcggta ttatctgcag attcgtccgc tgtatctgaa aaaccgcctg
     2041 cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc aagctgttct tgggctgtaa
     2101 aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc atacgcgctt
     2161 ctttttgctt ttgctcatat tggctgactt cttcttctgt atagatattc aggcagtatt
     2221 cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg accgccgcaa ttaggacatt
     2281 tatgtgtaag aacatccatc agctgcacac ctttctaaat aaattaatta acggggattc
     2341 ctttaaacgg tttaccacag tttggacaga atttagggat gccgttggaa agatccacga
     2401 tctcattgca ttcactgcat cgcatttgta gcttaggctg aacgttttct acaggtttgg
     2461 cagctccgca gttggaacag aattttccgg aattttctgt cccgcaaact ggacatgtcc
     2521 aagtatccgc atttccttgt gcagtttgtt gattttgttt ttctgcttgt tgtttgattt
     2581 gttcttggtt attttgagct gcttgagaaa gataagaacc atttgcattc attcccatac
     2641 ctacacccat aaagccggtc attgctcctg cttcattttt tccggctgct tccataccac
     2701 gtgcaatgga accttgcacg tatccttcac gtacgctagg atcacctagc atcgcccctt
     2761 cgttacgcat attgatcagt ttcacagaat catcggtgta agaaatgctg gctacagcca
     2821 cagaaacaat ctccatccca cgtaattcac gccaagaatc atccaatgca gtatccatgt
     2881 atttgcttaa ttccaaactc ttagatggta cataagaaat ccgttgacca tctgcagaca
     2941 tctgattgat tgctgattgt agcgctgtta ggaattcagc taaatactgc tcattgatat
     3001 cattgatttc cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat aaaatcgggt
     3061 caacgacatg aatagaataa gtgccatgtg cccgtaagaa taattctgcg ttatagaaat
     3121 tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat tccctttatt tcttggagat
     3181 tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat cggtcaaaag
     3241 tttcagcaat tgcttctttc agtgttccat tgaacatgga aggagctgcg tcatttttaa
     3301 tggtgtaata accttcctca gcggtataat cgatgatttt tccaccatcg accaaaagca
     3361 tcatcatgtt tggatagaca tggacgactg ttccgtctgt taaaacatct tctgttcctt
     3421 tacgattgga gccacgtttg tcatctttgc ggactttgac accttttgtc atgaccgttg
     3481 tatcactcat attgtctggt tcgatgactt ctagccattg atctgccaga ccgccgccaa
     3541 ccatacttgt tgctgctttg ataagaccca taaaaaattc ctcctctgat tcagctagtg
     3601 ccaaacaatt attgtttatt tattatacca taggaagatg tgaaacttct cggactagag
     3661 acggaattga aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg agaaggatag
     3721 tagttttttt acctaatatc acgtataata agagatagtg aattttttaa aaaaggggga
     3781 tccttatggc atatcaagca ctttatcgag tctggcgttc gcagcgtttc gaagacatcg
     3841 ttggacaaaa agctgtgaca cagacactaa aaaatgctat tgtttcccac aaaacctcac
     3901 atgcctactt gtttacagga cccagaggaa ccggaaagac gagtgcagca aagattttcg
     3961 caaaagcaat caactgtccg aacagtaaag acggtgagcc atgcaacgaa tgcgaaatgt
     4021 gtcgatcaat tactgctggt acgcaagaag atgtgatcga aatcgatgct gccagcaata
     4081 acggagtcga agaaatccga tttatccgtg atcgggcaaa ttatgcgcct acaaaggcag
     4141 cgtacaaagt gtacattatt gatgaggtac atatgctttc aacaggagct ttcaatgcct
     4201 tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt catcttagca acgaccgaac
     4261 ctcacaaaat accagctacg atcatttcac ggactcagcg atttgatttt aaacggatca
     4321 atacggcaga tattgtcgaa catcttgaat ttatcttgaa agaatcgact atcagctatg
     4381 aaccagctgc attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg gatgcattga
     4441 gtattcttga tcaggcaatc tcgtttagtg aaggaacaat tactttagat gatgccatgc
     4501 aagtgacagg tagcttaact gacgaaatga tggacagtta cttgagtgct tgcgtgaaaa
     4561 aagaagtacc agaagcattg gaaacattga accaaattct cgcagccggc aaagaatcac
     4621 gtcgctttct agaggatatg ttacaatact gccgtgactt gctgatgtat cagcaagcac
     4681 ctaacctagt tgaggaacaa tcgagtacga tcagaagtac atttacagaa ttggcacaaa
     4741 ctgtttcagc ggatcagctg ttttcaatga taaagatttt gagcgatact caaaatgaaa
     4801 ttcgttttac caatagtgcc gctatttatt tggaagtcgc tacagtcaaa cttgcacagc
     4861 taccgtctgc ttctgttcct tcagcagatg tagcatttgc agatgatcag atagtaaaac
     4921 agctacaaca agagattaag gaactaagaa acgaaatcaa ggaactacgc agtaatacag
     4981 gaattgagaa tacaaaagta cagccggtac agcaaacaaa tcataaaaaa cagacaagta
     5041 cctatcgtgt accgacggaa cgagtatata aagttctgcg ggaagcgaca agacagcatc
     5101 tgaaccaagt aaaagaagta tgggaagact tgctgatgag tctatctgtc actcagcgag
     5161 caatgcttaa agcaagcgaa ccagttgcag ctggacctac aggactagtg attgcgtttg
     5221 attatgagat tgtttgtcaa agagcagcaa atgacgaaga attgcagctt tcggtacaca
     5281 acaacttaag ccgaatgatc aaagattatg cgcctgatcc tgtttatatt acaagagaaa
     5341 gctggccaga acttcgaaga aattatttga cgcaggcaca agaagaggga acagaaaact
     5401 cttttgcttc tgaagactca gaaggagaaa tcgagttgct tcctcaagaa caagaagtac
     5461 cagaagaaac acttgtagta aaagcagagg aactgtttgg ctccgaagct gttacagtgg
     5521 tagatgatta ataaaaagga gtagataatt atgatgcgtg gaatgggcaa tatgcaaggc
     5581 atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga gctgctaaat
     5641 aaaacagagt ttacaggtaa agccaccaat gaattggtca aagcggtttt tacaggcgat
     5701 cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg atcctgatga tatcgagatg
     5761 cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga tgtggaaact
     5821 gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat tttagtttta aactgaagga
     5881 gtaatgaata tgcaatatcc agaaccaatt gcgaaactga tcgacagcta catgaaactt
     5941 ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt atacgatcga tatgaaagat
     6001 gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa aacgcgattt gcatttttgt
     6061 agtatctgcg gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa tccttcacgt
     6121 gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg taatggcaat ggaaaaaatg
     6181 cgagaatatc acggacttta tcatgttctc catggtgtgt tatcaccgat ggaagggaca
     6241 ggtccagaag acatcaatat ttcttctctg ttgcagcggc tgcatgatgc agatgtaaaa
     6301 gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag caacagctat gtatctctca
     6361 cgtctgatca aacccgcagg tatcaaagtg actcgtctag cacatggtct gtctgtaggc
     6421 tctgatatcg agtatgcaga tgaagttact ttattgaaag ctgtcgaagg aagacgtgag
     6481 ttataaattt ctaattaagt gttttttcag atacaatata aaagcagcac aagagatggg
     6541 ggcaacaatg tgaacggact atttattaca atcgaggggc ctgatggtgc aggaaaaacc
     6601 agcatcttga atgaacttta ccctcttttg aaagaagtag caaaaacaga aatcattcaa
     6661 acaagagaac caggaggcat tccgattgct gaagagatcc gagcagtgat ccttgatcca
     6721 aaaaatgatc ggatggatga acgtacggaa gcactgcttt atgcagcagc aagaagacaa
     6781 catctagtcg aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt atgcgataga
     6841 tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt taccgagatt
     6901 gctcggttga atgagtttgc tacagaaggt actacgccgg attttaccct ttatcttgac
     6961 gtagattcag acaccggatt aagaagaatc aagaaaaaca gacaaaatca aatcgaccgt
     7021 ttagattcgg aaggtttgga attccaccag cgtgtacgtc atgcttactt aaagctggca
     7081 gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa aaagctttga agaagtgctg
     7141 caaacaagtt atcatacaat tattgaacag tatccgcaat tttttgaaaa ctaggaaggt
     7201 gaacgtttat gaaaattatt ttagcgatta tccaagacaa agacagcaac cgtttatcaa
     7261 atgaattgat tgatgcaaat atccgtgcca caaaactatc atcgacaggc ggcttcctta
     7321 aggctggaaa cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa gaagcattgg
     7381 aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt atctacacct gttacactgg
     7441 atatttcgat ggatgggggc gtcccttatc cagtagaagt agaagtaggc ggtgccacag
     7501 tcttcgtctt acctgttgaa ggattccatc aattttagga ggagctgagc tatgaatcaa
     7561 gaaataaact tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg gagttttgag
     7621 catgggcgta tcgctcatgc ttatcttttt gaaggggaga aaggaacagg aaaacatgaa
     7681 gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa tctgccttgt
     7741 ggaaaatgca acaattgtca gcggatcgaa aagaaagaac atccagatgt tttggttatt
     7801 gaaccggaag gacaaaccat caaagtcgat cagatcagac gtttgcagac ggaattcagt
     7861 cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca aagaagctga aaaaatgaat
     7921 agcagtgcgg caaacagttt gttgaaattc ttggaagagc cccctggaga ttttttagcg
     7981 gtgttagaaa cagatgcagt aggaagaatt ctccctacta ttcaatctcg ttgtcaagtt
     8041 ctgcattttc aagaactttc gaaagaagca ctgatccatc gattgcaaga agagcagatt
     8101 ccattggaaa aagcaaaact tttggctttc ttaacaaaca gtttaggaaa agcagttgaa
     8161 atctctcaag atgaatggtt taatgatgct aaggacttga tttatcaatg gttcgtttac
     8221 ttgcaaaagc aagatactca agcctttatc tatgtccaaa aaaaattggt aaaatcattt
     8281 aaagaaaaaa cacaacagtt tacaggatta tctgtcttga tgttttatta tcaggaagca
     8341 cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga aacagataaa ccagataatt
     8401 gaacggatat tgatcgcaga gcagaaacta cgcagcaatg tcagttttca ggctgttgcg
     8461 gaacagttcg ttttgcatac gattttttag cttgccgcaa aggatgatga tcaaggagga
     8521 tggaaatggt agaagtagta ggtgtccgat atagagatgc aggtcatatc tattattttg
     8581 caccaggaga ctctgaatat gtttatagtg acaaggtatt agtcgaatct cagcaatcct
     8641 tacatattgc tactgttgtc cttcctaaaa aagagattga tcccaatgac cttcctgaag
     8701 ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt agaaaaagac aaaaaaaaca
     8761 aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg cgtgagttgg
     8821 aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt ttttctttca
     8881 cagcggacgg gcgaattgac tttagagaat tggtaaaaga tctggccgct gtatttcgta
     8941 ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc aaagataata ggcggaattg
     9001 gtccatgtgg gcgtccatta tgctgttctt cttttttagg cgatttcgtg cctgtatcaa
     9061 tcaaaatggc aaaagatcaa agtttatctt tgaatccaac aaaaatttcc ggtttatgcg
     9121 gacgcttgat gtgctgtctg caatatgaga gcgaagaata cgagacagcg aaaaaagaac
     9181 tccctgactt tggaaaagta attgctacac ctgatggtaa agggaaagtc gtagggttga
     9241 atctgcttag ccgtatcgtt agtattcggc tgactggacg ggaaaatcct gtcgaatatg
     9301 aatgggatga attgaaagaa tttatccaag taggtgagat gaatggataa gagatcatta
     9361 tacgacggat tgaatcaatt agaatcagag ttgagaaata cattaacaga acttgttgaa
     9421 atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa ctttggagat ggaaaatcaa
     9481 cgtctgcggg aacacctgcg ggaattgaac caagttgcac aagctccagc tgaaaatgtg
     9541 aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga aggattccat
     9601 gtctgctacg atttatatgg atcaagaagg gacaatgacg agccttgtgc gttttgtcta
     9661 gaagtgatct atcgcgaaag cggtcgatga ccaataacaa gaggagactg gagcggagaa
     9721 atactgctcc agtcttttta ttaaggagat tgtgacagat gtataatcaa aaaagtttca
     9781 agggaaatcc ggtatatggg gatttatatc ttgtaccgac gccaattgga aatttagaag
     9841 atatgagcta cagaagtgtt cgtatgctgc aagaatccga tcttatcgca agtgaggata
     9901 cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa aaaagtttac
     9961 atgaacataa ttataaagaa cgtgtccctc aattgattgc tttgttaaaa gaaggaaaaa
    10021 cgattgctca agtcagtgac gcaggaatgc cttccatcag cgatcctgga catgagctag
    10081 ttttagcctg cataaaagaa gggatttctg tgatctctat cccaggacct acagctggga
    10141 tgacggcttt gattgcttca gggctagtgc ctcaaccatt tttattttat ggcttccttc
    10201 cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct aaaagaacag acagttacgc
    10261 tgatttttta tgagtcacct tatcggatca ctgctacatt gacgaatttc ttagaaattt
    10321 ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa gaatacctta
    10381 gaggaagtac ggaagagcta ctcgcttata tcgaagaaca tccagtaaag ggtgaatgtt
    10441 gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac ggaagttgat gaagagttgg
    10501 gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca aatgctgcta
    10561 tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga agtctaccgt cagtaccatg
    10621 aattagactg agatagtata agttgtctat aatgaaagac taatgaggaa atcagctact
    10681 aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta taatgaataa gaggtgagga
    10741 tgatggagct gcaatttcct ttacataatg atacatcaag aaaaatcatt catattgata
    10801 tggatgcttt tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc ggacatccat
    10861 tagtaattgc cagacacccc agtgacactg gtgggcgtgg agtagtgact acagcaaatt
    10921 atgaagcaag aaaattcgga attcattcag caatgagtgc acaaaaagcg tatgagcttt
    10981 gtccgcaagc catctttaag cctggcgacc atcataaata cagcgatatt tccaaacaag
    11041 tacgggaagt attctatcgt tatacagata ttgtggaacc agttagtatc gatgaagcgt
    11101 atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc gcccgaatga
    11161 tccaagcaga tatttgggag agtgtccagc taacatgttc tgcgggagta agttacaata
    11221 agtttttggc caaattagca tcagactatc aaaaacctag aggaatcacg gtagttgccc
    11281 ctcaagacgc tgtgccattt ttaaaagctt tgccaattga aaaatttcat ggggtaggga
    11341 agaaaactgt acctagaatg catgagctag ggatttatac aggaaaagac ctatatgaat
    11401 gcacagaaat gatgttgatt cgaaattttg gaaaaatggg ctattctctt tatcgtaaag
    11461 tcagaggaat ccatgattct ccagtaaacg ttacaagaga gcgaaaatct gtgggaaaag
    11521 agcatactta tgggcagccg ttgcaaacgg aagaagcagt gctgactcag ttgcgccaac
    11581 tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa acatgggaaa acagtagttt
    11641 tgaaggtcag gtacacggac tattctactg tgaccaaaag agtgacattg ccagaatata
    11701 tctataaaaa agaagcactc ttttatcaag caagccttat atgggaggaa atcttgggag
    11761 tagaaaaagg aatccgattg ttagggatta ctttgacgaa tctggacccg atgacttatg
    11821 aaaacatcgt ccttccatta tgggaaaatc aagaaatata aaaacagctt tgagagttga
    11881 aaccggaaat ccgttttctc tcccaaagct gtttttatat caagtcttgc tttttgattt
    11941 gttcataggt ttctgttgca tccgtacatt tttcccaaat cacaacttga ttggattcta
    12001 aagatttttg gacgtcaatg atccgctggt tgctacttcc gcgaaattgt agttttaggt
    12061 cacgtttgct taattcaaag cgtccgtcaa caaggatatc gatttgcgaa aggagttcta
    12121 atttatcttc tgaatcttgt agcaattcat caaaggtata gcctgtccat gaccagatat
    12181 cttttgtcgt accgaactct tttcggatac gtcgtacgac ttgcagacaa acatctgtat
    12241 tcaaaaaagg ttcgccacca agtaaagtta atccttgcac gtaatcgttg cgcaagtcag
    12301 cgatgatttg ttcttctaat tcaggtgtga atggtttgcc atatcggaaa ttttgtgcag
    12361 ctttgttgaa gcagccttcg caggcaaata agcagccact aacgtaaaga ctgttgcgga
    12421 caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt tggctgtagt
    12481 cttttgctaa ccattctttt ggttttggat ttctcataaa aaaacttctt tcatttaagg
    12541 ttcaaagata tagctattga agataaaatc tactcgacca cataaataag aacaaactga
    12601 cctaaatcca ttgttcaaga aagcttgttc ttattttgat agagggcgtt caaagataca
    12661 aagccatcga aagagggtgg tgaaaaaaga cttgtcatag cctcttattc tgaataaacg
    12721 gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa acgaaaaata tgaggaacta
    12781 tttttgttta gctattctta ttacttgatg caggacagct ttttcacaac ctcatttcat
    12841 gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt accatcggac gagcttgcgg
    12901 atttcctaaa tagccacaag tacgtttgac tacatcacat gtttttggat catgatttcc
    12961 acattctgga cattcgaacc ctcgttctgt cggtttgaaa tcaccttcga aaccacattg
    13021 atagcaatga tcgattggcg tattcgtacc aagatacccg attttgtcat atgcaaaatc
    13081 ccatacagct tctaaagctt tcggattttg ttggagcata gggtattcac agtagtggat
    13141 gaatccacct gaacaatatt ttggataatc cttctcaaat tccaattttt caaatggcgt
    13201 tgggttttta cgcacatcat agtggaaact gtttgtataa tattctttat ctgtgatatt
    13261 ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa cggtcagtta gactttcact
    13321 tggagttgaa tagacactga aatgataacc atattcatcc ccccatgcat ctgcattagc
    13381 ttttaattct ttgacgatat caagtgtgaa agcttttgct tctgggtttg tttcccattc
    13441 gccaccgaag aaagctgaag caacttcata caatccaatg tagcctaacg atacagtagc
    13501 tcgacggttt ttgaataatt cattgacaga atcggtacgt gaaagacgtt tcccgaaagc
    13561 accatacata tataaaattg gcgcatttgc tggaatggct tctttacaac gttctacacg
    13621 atagagaagc gcatctttca tgatttctaa gcggtcagac agtaactgcc agaatttatt
    13681 ctgatctcct tttgattcga tcgcgattct tggcaaattc aatgtaacaa cacctaaatt
    13741 cattcgtccg acatttactt cttgaccatt ttcgtctttc cacccttgca agaatgaacg
    13801 acagcccata ggtactttga aacttccagt taattcaacg attttatcgt aattcagaat
    13861 atcaggatac attcttttcg ttgcacattc caatgctagt tgtttgatgt cgtaattagg
    13921 atcttcaggg tttaagttca cacctttttt caaagtgaag atcagtttag ggaagatagc
    13981 tgtccgcttt tcactcccta atccattgat tcgaatctgt aaaattgctt tttgaatttc
    14041 tctttcaaac cagttttctc ctagtccaaa tcctaatgaa gtgaaaggag tttgtccatt
    14101 tgaagtaaat aaggtattga tttcatattc caaactttgc atagcatcga agatatcttt
    14161 tttggttttt gcttttgcat attctttttg gcgttctggc gaatcgatcc attcttccgc
    14221 gtcttttaga tgttttttat agttcaattc tgcaaaagga gcaagcaatt catctgttcg
    14281 gtcggcagaa cagccaccat attgacttga cgctacattg gcaataatct gtgagatttg
    14341 tgctgtggcg gtctggatag attttggcga ttcgacttcg gcattgccga ttttgaaccc
    14401 attgttcagc atgcctttga aatcaatcaa gcagcaattc gtcatcggtg tataaggatg
    14461 ataatccaaa tcatggtaat ggatatcgcc tttttgatgc gcatttgcta tatgtggtgg
    14521 caacattttc agtccgattg attttccaac gatcccggct gtcagatctc gctgtgtatt
    14581 gaaaacatta ctgtctttat ttgcgttttc gttcacgaca gtttggtcct tgttgattag
    14641 cttttcaatc gaaaagttga tatctgttgc tttactgcga gcaaaatctc gtttggtacg
    14701 ataattgatg tactcttctg ctaattcata ttcgtttttt tctagtaata tatgttccac
    14761 gatgttttga atctcataga ttttgacatt ttcagcaaac cggcttttga tttcttggac
    14821 aaccaagtct gcaatcattt gaattttttc ataagtaagt ggtgataatg agccgtgaat
    14881 tttcttttct gctttgatca gtgcttcatt gatcttttgt tcatcgaaat caacatggcg
    14941 gccatcgcgt ttgatgactt tcatctggga tgtctctggc tgctgagtaa cgacagtttt
    15001 ttcttcataa tttactttca ttatcttcaa ctcctgctaa attgttcttg tctatcataa
    15061 aacaaaatcg gggaaggagc aactaaatat cggctatata tagtattcaa tgtgctgtat
    15121 aattggattt ctatactata tgtagtgtat attttattgt gaaaaaaggt gaaaacttgt
    15181 agctggtgga tttgtttttt gcatatttaa ttttgctgaa taaaataatt gaataaaatt
    15241 ttttattcag tccaagtaaa agtgacaaaa acataagagc aaattattcg tagatatgct
    15301 actatgcaaa taaatagaaa tgaaaaatga aagaggagga gaagaattgg aaaagatgat
    15361 cgaagtgaaa catctaagta aaagctacgg cagcaaactt tctcccgtca aagccttaga
    15421 taatatctca ttcacagtag aagaaggcga gtttgttggg attatgggcc caagtggtgc
    15481 aggaaagaca acgctgatga atatcttgtc cacgatcaat ttgcctacga tgggcagtgt
    15541 ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg actttcgtcg
    15601 aaaaaaacta ggattcattt ttcaagagtt caatttgatc gataccctaa atgcaaaaga
    15661 caatatccta ttgcccctag cagttgaacg gatgactaaa gaggaaatgg aaaagagagt
    15721 aagacatgta gctcaattgt tgaatataga agaactgctc aaacggtatc ccgatgaact
    15781 ttctgtcgga cagagacaac gaattgcagc tgctagagca cttgttgttc agccacaagt
    15841 gatttttgca gatgaaccaa ctggctcttt ggactcgaag tcggctacag aattgttgaa
    15901 ctacttgaag accatgaatc aaaaagaaaa agcaacgata ctgttagtca cccatgatcc
    15961 atatacagca agctactgcg atagaatttt atttattaaa gatggtgtga ttttttctga
    16021 agttgtcaga agagggacaa gaagagagtt ctttgagaaa gtaatagaca tgcaggcgac
    16081 gattggcgga ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt ttttttcggc
    16141 agtcacgagg ctatcttgtt tattttttca gtttgacttt atccgtaatg atctattatt
    16201 ccttcagcgc aatgacctac gatcaatctt tagtcagaag agcaagtcaa gatacttcga
    16261 tagatggtgg attgagtttt ggtggattta tcattactgt tgttttgctt tttttcgtat
    16321 tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga aataggcatc tatcagcttt
    16381 ttggtatgaa taaactgcag atatcaggta tatatgtgtt agaaatcatg ataattggat
    16441 tgttcgcctg tatttctggg atattactcg gtattatttt ttccaaactc ttttcaatga
    16501 ttttggttcg aatgatggat atggatctta ctagtccatt ttttatctcc attccatcag
    16561 tagtagatac tttatttgct tttttcatca tccttttagc agtatctgta tattcaatat
    16621 ggaaaatatg gcgctatcca atgattcgaa gttttggaga aaaagaacaa gcagatagtt
    16681 caacaatgcg cttcaggaca aggcaccgat tactcgcagt catcgggtta ctacttttaa
    16741 cgagcggata ttttggagct agtcatttta gggaaatcac ttcatggatg atcagttatc
    16801 attatcttga tacattagtt ttcttttttc cttttttaat ttttttcgtt tgtgtggtcg
    16861 gtacttattt gtttttctgt tatacatttc ggttattttt gatcatattg agtaaatgga
    16921 agatttatta ctatggtatc aattttttga taatgggtaa cacgcaagtt catttgctca
    16981 aaagttggcg tacaaattct ctgattacag tcattctcgg aatttcttta atgacaattg
    17041 gcggcacggc tagtatctct actattttat cctaccagga gaaagtttcc tccccgatgg
    17101 actatcagtt tgatgttgag acagagaaaa aaatcaagcc gattctagca gaggaagggc
    17161 agaagataac taaagaaatc accttgcatt ataaagtcat tgggagtatc tatagtcaaa
    17221 atctcgaaga acctatgttt caaactgcta atctgatcac ggaaacacaa tatcaagcct
    17281 tcagaaaagt taatccagat ttgccgaaca tttctttgaa atcacctggt catactgttt
    17341 tgcttgatca aatgcaaact ctttttcgga atatttcatt atatggatct agtattggac
    17401 tgccagataa tcaagaattg aagattcaac aaatgcttcc aagtgtatta ggtgacgaaa
    17461 caatggtata tctaagtcct gtactgattg tagatgatac cgtttttgaa aaagcaaaag
    17521 gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa aaaatagatg
    17581 aacgactgag caatgacctg tcgataaagt ggctcaatgc tgtttattat tcttatgata
    17641 tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa acttccggat gaccaaatga
    17701 aggaagcaca atttagccaa gaaagctctc gtttgaattt ctcttccagc tattcgaaat
    17761 tgcgtgcagt taatcggcga atcggtatca atatttttgt cacactattt gtcgggatga
    17821 tcgtgattat tgctactggt agtattttga cagtccggca attagcagaa gcagaggaag
    17881 aaaaagaaaa ttatcaccta ttgactaagc tggggattcc acaaagaagg atcagacgga
    17941 tgatcttcga gcaaaacgca cttaccttct ttccacccat gatattaggg atcactcacg
    18001 cagtatttgc aatcaatgtc ttcactcagt atataaaaac tgccgattac tggttggctt
    18061 atcttgtgag tggcttattg atccttatct atcttttgct ttatgtcatt acgagtcagt
    18121 tatattgtcg gattatcgag gaatgatata cggaataaga caagataata aatagaacta
    18181 aattttgaat accatttatt tggaataaga ggttgtgaca cgatttttgt cacaacctct
    18241 ttatttttta tattttttaa aagtacgaga aattataaag aaaaaagcag tgatttgatg
    18301 tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt tctgtaagct
    18361 agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat atgaggaggg
    18421 aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa attcatcatt
    18481 cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca ctataaaaat
    18541 attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg aaatcaaaac
    18601 gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg cttatcaggc
    18661 ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg cttattattt
    18721 agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg agtttgaaat
    18781 gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga ttgaagagca
    18841 tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta tggtcatcag
    18901 tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa aagaatttca
    18961 agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag gtacagaaaa
    19021 tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac aaatcagtca
    19081 tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca agcctggtat
    19141 gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta tttatcctgc
    19201 attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag actttcgtca
    19261 attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc catcatttat
    19321 ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat tggacatgtt
    19381 tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg atcgattccc
    19441 aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta tttcaagtat
    19501 tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct acgtaaaaaa
    19561 agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag aaacgggtga
    19621 aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg aaaagacagc
    19681 tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg caggtagact
    19741 agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca aattacatgg
    19801 ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc gaatcaaaca
    19861 agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg cttttgtcgt
    19921 attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca aagaacaatt
    19981 actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg atctgctgcc
    20041 tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga atcctgaatg
    20101 atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt agccattgcg
    20161 cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt ttatcaatca
    20221 gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca acagggagcg
    20281 gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt tgtttatcgg
    20341 caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat aattccactg
    20401 tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact tggtttttta
    20461 ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag agatggcatg
    20521 atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc aacaatctct
    20581 tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc gcctgttcgt
    20641 gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg ttttttatat
    20701 aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg acgcattgcc
    20761 ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta cagtatgtat
    20821 ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta catgatggga
    20881 tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa agaattctgg
    20941 aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat gcgattgatg
    21001 ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa tatcggttat
    21061 tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta catcgtatat
    21121 ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt taagaagaag
    21181 cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt tttgacattt
    21241 caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact tttttttagc
    21301 taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg gaagaacaaa
    21361 ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat cgtgatattg
    21421 atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta gtcgagctag
    21481 acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat tggagcacac
    21541 cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa agaaactttt
    21601 tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt ttacctcctc
    21661 ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt ccatgtcagt
    21721 gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac aatatattcc
    21781 tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg ttctggcaga
    21841 aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac aatcgttaag
    21901 ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag tcgtctttat
    21961 tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta atacgtatta
    22021 ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc ctgctgatcg
    22081 ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata cgttgacggc
    22141 gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga atcagctgcg
    22201 cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg ggttgaaaga
    22261 tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc aagaaacaga
    22321 attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc cattcgggtt
    22381 gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca aacagtcaca
    22441 aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg tcttggatca
    22501 attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg agaaatggag
    22561 cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga tcaaatttca
    22621 attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg gtaccaacta
    22681 ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag atcaacagat
    22741 cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg acgatgcttt
    22801 ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat aaagaaaaca
    22861 ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa gataaaaata
    22921 ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga atatagaaat
    22981 gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac ttctgcaaaa
    23041 ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc caaaccattc
    23101 gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg tgctggcgga
    23161 ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt aacaaaaata
    23221 accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca agtcatgctt
    23281 gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt gattggttcc
    23341 aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat aaaggatatg
    23401 ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat acgtcctgga
    23461 gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata tttactggca
    23521 aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga gaatctatct
    23581 aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga agtaggcttg
    23641 cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc tttagatact
    23701 ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat tttaggaaaa
    23761 ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt attgaatttt
    23821 gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat ttctggcgga
    23881 ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt agccattacc
    23941 gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga ttatttgaat
    24001 acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt tgaaaaaatt
    24061 cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa aaagcacgag
    24121 tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct ataatattta
    24181 cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa agcatgcagc
    24241 agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac tatgcattgt
    24301 tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt ttattttcag
    24361 ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat tcagtcatca
    24421 actggctact caacagttta atctttctgc ccatgttttt aatcagcgga gtgagtactt
    24481 atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca gaagattttc
    24541 ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca ctcatcatga
    24601 gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat cctgatttgt
    24661 cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt ggtacgtata
    24721 ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct ttgttcatcg
    24781 gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat gaacaagcac
    24841 ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt ttattcatca
    24901 aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg aaattaactg
    24961 ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt tatttgcttc
    25021 aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc aaattacaag
    25081 aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat catgatcata
    25141 ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga aatccggaga
    25201 cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa ttgacgttgg
    25261 aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt ggtccttttc
    25321 tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt atcgtagaag
    25381 aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa agtttcgctg
    25441 attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa ggaaatgaac
    25501 atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca gctaacgtaa
    25561 agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg aaaaatgaag
    25621 cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac aaagtgttta
    25681 acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc aagaatcaat
    25741 ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat tttgctcttt
    25801 tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga agaaatcaat
    25861 cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg aggcggagct
    25921 gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga tggggattca
    25981 ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga attgggtgtt
    26041 gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa attttctggc
    26101 aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat gttcgactct
    26161 gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga atcaaaagga
    26221 atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga agaaaagcaa
    26281 aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg cgtagagtca
    26341 gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat caatcgaatc
    26401 tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga tttcaatttc
    26461 tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt ctcagaagga
    26521 atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat ctctgacgtg
    26581 gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat agtcagccaa
    26641 attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac cttactttat
    26701 tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta tacaggattc
    26761 gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt ttcctgcgtt
    26821 agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa acgttcctga
    26881 tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg tgcactatgt
    26941 tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat ttttcgatac
    27001 agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg gatcaaaagt
    27061 tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg gaaggttatc
    27121 tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt tgtcacctgg
    27181 tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat cttttccaca
    27241 tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc catctcttgg
    27301 acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg ctcataatgt
    27361 tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc tttgtcggta
    27421 tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat aatatataaa
    27481 atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat gtaaatcaag
    27541 caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa acggttattc
    27601 ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac actagcaata
    27661 ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata aagcagaacg
    27721 ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt atagtaaaac
    27781 attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa taaatgagat
    27841 aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc tgatacagct
    27901 tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc aagacgtttt
    27961 tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa aacagcgatt
    28021 atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa tggtccagtt
    28081 gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga tgagactgta
    28141 agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa tgagagtccg
    28201 ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc tagatataca
    28261 gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg tttttcaagt
    28321 gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg agtgtacttg
    28381 ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc ttttatttct
    28441 ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc aattcctgtt
    28501 aaaaataaca atgataatat aatgattatg ctccacttta tacgtaaata aaaagattct
    28561 tttttattat atatgttatt aaaaaaagac ataaaggtat tcctttctaa aactt
//
LOCUS       DAFFFQ010000028        28599 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000028
VERSION     DAFFFQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.028"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            234..1874
                     /locus_tag="LQB69_001330"
     CDS             234..1874
                     /locus_tag="LQB69_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL3525505.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2028..3350
                     /locus_tag="LQB69_001331"
     CDS             2028..3350
                     /locus_tag="LQB69_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL3525506.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3412..3843)
                     /locus_tag="LQB69_001332"
     CDS             complement(3412..3843)
                     /locus_tag="LQB69_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525507.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3907..4743)
                     /locus_tag="LQB69_001333"
     CDS             complement(3907..4743)
                     /locus_tag="LQB69_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL3525508.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4933..5235)
                     /locus_tag="LQB69_001334"
     CDS             complement(4933..5235)
                     /locus_tag="LQB69_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL3525509.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LQB69_001335"
     CDS             complement(5261..5692)
                     /gene="ruvX"
                     /locus_tag="LQB69_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL3525510.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5689..5955)
                     /locus_tag="LQB69_001336"
     CDS             complement(5689..5955)
                     /locus_tag="LQB69_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL3525511.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6209..6934)
                     /locus_tag="LQB69_001337"
     CDS             complement(6209..6934)
                     /locus_tag="LQB69_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL3525512.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7300..7370)
                     /locus_tag="LQB69_001338"
     tRNA            complement(7300..7370)
                     /locus_tag="LQB69_001338"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7336..7338),aa:Gly,seq:ccc)
     gene            7487..8239
                     /locus_tag="LQB69_001339"
     CDS             7487..8239
                     /locus_tag="LQB69_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL3525513.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8255..8734
                     /locus_tag="LQB69_001340"
     CDS             8255..8734
                     /locus_tag="LQB69_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL3525514.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8985..10565)
                     /locus_tag="LQB69_001341"
     CDS             complement(8985..10565)
                     /locus_tag="LQB69_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL3525515.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10558..11301)
                     /locus_tag="LQB69_001342"
     CDS             complement(10558..11301)
                     /locus_tag="LQB69_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525516.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11477..12253)
                     /locus_tag="LQB69_001343"
     CDS             complement(11477..12253)
                     /locus_tag="LQB69_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3525517.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12501..13337)
                     /locus_tag="LQB69_001344"
     CDS             complement(12501..13337)
                     /locus_tag="LQB69_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL3525518.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13348..14277)
                     /locus_tag="LQB69_001345"
     CDS             complement(13348..14277)
                     /locus_tag="LQB69_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL3525519.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="LQB69_001346"
     CDS             14470..14823
                     /locus_tag="LQB69_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL3525520.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="LQB69_001347"
     CDS             complement(14897..15553)
                     /locus_tag="LQB69_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL3525521.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="LQB69_001348"
     CDS             15779..17713
                     /locus_tag="LQB69_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3525522.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="LQB69_001349"
     CDS             complement(17799..19022)
                     /locus_tag="LQB69_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3525523.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20914)
                     /locus_tag="LQB69_001350"
     CDS             complement(19016..20914)
                     /locus_tag="LQB69_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL3525524.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="LQB69_001351"
     CDS             complement(20908..22026)
                     /locus_tag="LQB69_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL3525525.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="LQB69_001352"
     CDS             complement(22109..22942)
                     /locus_tag="LQB69_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3525526.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="LQB69_001353"
     CDS             complement(22935..23852)
                     /locus_tag="LQB69_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3525527.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="LQB69_001354"
     CDS             complement(24079..25203)
                     /locus_tag="LQB69_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3525528.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="LQB69_001355"
     CDS             25386..26294
                     /locus_tag="LQB69_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525529.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="LQB69_001356"
     CDS             26435..28048
                     /locus_tag="LQB69_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3525530.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5620 c         4868 g         9219 t
ORIGIN      
        1 tatttatcta tctaacatat cctttattct tctacttttc tttctttagc atataagaga
       61 tacgcatagt aatcaagcaa ggattgttga tataaaaaga acaaataccg ctattttgat
      121 ttcttttgac tctattactc atataaataa aatttattat acctgctctt tttgactata
      181 tcctcatctg actcctatac ttcgtttgta ttaaaactta ggaggaatca ttcatgctta
      241 cagggctaaa cttattaaat aatccttttc taaataaagg aacagctttt acaaaagaag
      301 aacgggcaaa atacggaatc actggtatgc ttccaagtac tgtccaaaca ttggaacaac
      361 aatctgtcca agcttacggt caatacttaa gcaaacagac cgatttggaa aaacgtatct
      421 tcttaatgaa tctattcaat acgaaccgga cattgtttta taaattgatg ggcgaacatt
      481 tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc ggattcaatc gaacaataca
      541 acgaaatttt cttgaaacca caagatgctg tatttctttc aatcgacgaa ccagaaaata
      601 tcaaagcaag tttgaaaaac gcagcagatg gacgcgacat tcgtttgatc gttgtgacag
      661 atgcagaagg aatcttaggt atgggtgact ggggtgtgaa cggtgttgac atcgctatcg
      721 gtaaattgat ggtctacact gccgcggctg gtatcaatcc tgcacaagtc ttgcctgtat
      781 caatcgacgc tggtactaat aacgaagaat tattgaataa tccattatac ttagggaatc
      841 gtcatgctcg tgtagaaggc gaaacttact acgaattcat tgatcaattc gttcaatcag
      901 ctactgaatt attcccagaa ttgttattac attgggaaga ttttggacga ggaaatgctg
      961 caacgatctt agaaaaatac gaagataaaa tcactacttt caatgatgat atccaaggca
     1021 caggaatcgt tgtactagct ggtgtattag gtggattgaa tatatcaggt gagcaattga
     1081 aagatcaaac gatcttgaca tttggtgctg gtactgctgg tgtaggtatc gcaaacatcc
     1141 ttttagatga aatgatccgt caaggtgtac ctgaagaaga agcacgccaa catttctatc
     1201 aagtagataa acagggttta ttgtttgaag atacaaaagg attgactcct ggtcaaatcc
     1261 cattcgctcg taaacgctca gaattcacta atgctgatga attgacaaac ttagaagcag
     1321 ttgtaaaagc aatccatcca acgatcatga tcggtacttc tacccaacct ggagcattca
     1381 ctgaagaaat cgtgaaagaa atggcggctc ataccccacg tccaatcatc atgcctttgt
     1441 ctaatccaac aaaattggca gaagcaaaag caaaagattt gattgaatgg acggacggaa
     1501 aagctttagt tggaacaggt atccctgctg ctgatgtgga atataacggt gtaacttacc
     1561 aaatcggtca agcaaataat gcgttgatgt acccaggttt agggcttggt ttgattgctt
     1621 caacagctac acgagtaaat gctgaaatca tttctcaagc tagtcgagca ttaggcggaa
     1681 tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc agtagcaaaa atcactgaat
     1741 tctctcaaac gattgctgaa actgtcgcaa aatcagttgt tgcacaaaaa ttgaatcgtg
     1801 aagaaatcac ggatatcaaa gaagctgttg aatctgctaa atgggttcct gaatataaat
     1861 ctttagaaga ttaagattgt tatagtaaaa gaacatacag aaactttgta tgacgtctaa
     1921 aaagaaaaaa gcgactgcgg caaaacagca gccagccaac tactgctctt ttgccgcttc
     1981 tctttggctt ttttcatacg atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa
     2041 caacaaccgc aactgcagtt gaggaaaaga aaaaagaagg gttttggtca acaaaaatta
     2101 ccggcgtaag tttgcccctt tatttgatca tggtccttgt cctgatcatt gcaatggctt
     2161 taggaaaatt accgacgaat atgattggcc caatcgctgt tttggtcatt ttcggtaatt
     2221 tatttcattt tatcggaaca aaaatcccta tagtcaaaag ttatttaggt ggtggctccg
     2281 tttttgcgat ctttgcctct gccgcgattg ctacatttgg gatcttacct gagtatgtag
     2341 taaaatccac agaaaacttt gtcagcaata tgggcttttt agacttttat attgctgcct
     2401 tgattactgg tagcatcctt ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt
     2461 ttattcccgt tgcacttatt tcaatggtcg tctccttttt tgccgtagga ctcgtaggta
     2521 tgcttatcgg aaatggtttt gcaaattccg ttttatatat ctcattgccc gcaatggctg
     2581 gtggtgttgg agccggagct gtacctttat caacgattta tgcaaatgtt ttaggtacgg
     2641 acgcttcttc tatcatttcc cgcctgattc ctgcgacagc aatgacaaat gtcctagcta
     2701 tcattggtgc agcgttagtt gctcgtgcgg gacaatcaat gccaaaattc aacggtaatg
     2761 gtaaattaat ggaaaaaggc gaccttggcg atggaaaacc tagaaatgta aaaccttcca
     2821 ttccacaatt aggtgtcggt ttgatggtat cattgacttt cttcatctta ggacaaatct
     2881 gtaactattt tgttcctaaa gtccatgctt acgcattcat gatcattatc gttgttatct
     2941 gtaaaatcgc aaacctatta ccagaatatt acgaagatgc agctatcatg ttcaacaact
     3001 tgattgtcaa aaacttgaca gcagctgttc ttgccggtat cggtattgcg ttattgaatc
     3061 taaatgtatt ggcttctgct ttgacatggc aatttgttgt cttatgtttg acaagtgtta
     3121 tcgcgatttc agtagtttca ggatttgtcg gtcgtttgtt tggtttatat ccaatcgaat
     3181 caactattac tgctggctta tgcaacaact cgatgggtgg tacaggaaat gtagctgtct
     3241 tatctgcagc taatcggatg gaacttattg cttttgctca aatgggcaat cggcttggtg
     3301 gtgccatcgt tttgatcatc tcaggtttct tgatgcaatt actttcttag tcaaaaaaaa
     3361 tacctgatca tcaagtatcg gcttgatgac caggtatttt ttgataaaaa tttacaggat
     3421 tttcaaaaat cttttttgca ttttatcgga atgataaccg atgcgttcat aaaatcggtg
     3481 tgcttctact ctgtattctg cagaattaag tcttattgct gatattcctc gtcttttcgc
     3541 ttcttctacg ccagtcatca aggcctttcc gattcctctt ttttcagcat gtttagacac
     3601 agcaagtgcc ataatattga acatcggatc gctgaaaatc gattcatagg actccgcttg
     3661 tacatagcca gctgcttttt tagaagtagg atcttcatag accaagaaaa aatttttttc
     3721 tggctccttc aacagtttct tcatttgtct caccgtaacg gttaatggta catcatatcc
     3781 taattgttcc gcattgattt ctcgtagtgc ttctgcatct cctaaacgca tttcccgcaa
     3841 catccaaatc accagccaat ttttattttt ttaaatgaat ttttttatca cagtatactc
     3901 cttttttcac ttaaaaaaag acttttcagt gattttgact tgcttgaatc cagatccttc
     3961 taatgtttgc aagatgacga aagcattgta aagattgatc aaaagaacca cgatgacaat
     4021 cgcttgaaaa ataaaggtca ctttttttgg agacaaaacc ctgctaaatc ttgctcccca
     4081 caaacctcca ataacagcag cggggataac ataaaacaac atagataaat caaagcgcat
     4141 aaagctactt gttaaagcaa tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga
     4201 tagagaatat agtgttgctt cttttattgg caatgcaaac atcagcatca acaaagaaac
     4261 attgactggt ccaccaccaa tacctaaaaa actagctaaa aagcccagaa cccccccaca
     4321 tataaggtac caagtccatg acgtcaaatg aaattttggc tgatgatgct tggtgtagaa
     4381 gaacgcaaaa accaaggtca ataccgtcaa aaaaatttgg atcagctgca catgcttctc
     4441 gttttcaaat aactgtaaaa aaacttcgaa tacaatgttg ccgactactc ctccaacaac
     4501 agctccacta gatacccaac cgacaatctg ccaattcagt gatttcccgc ttgatacttg
     4561 tcgcacagtc gaaacaatgg acatcgtgaa taccgcaact gttgagtaaa aagaaatccc
     4621 tgcaacagaa tgggcgccga taagatcaag aatcggtttg atcaacacgc ctccacccat
     4681 cccggaaata gcgccaactg tgttcgccag aacgatcacc acaaaataaa tcagaccaat
     4741 catcttgtat ttcctttctc ttgtttatat cttttagcga ttgttactct tttcttttat
     4801 tatacagtaa aagaaacaaa cgaggcttcg aaagcttttc tactttttaa ggaaatttag
     4861 tgctaaacga aaaaaccggg ctctactttt cagagagtcc agcttttctt acaccttatc
     4921 gattcttttg ttctattctt cttccgccat gaatgtattg aatacttctt cgatcatatc
     4981 ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt cgttttcgat
     5041 gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagctg gatataaaag
     5101 aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agcaagattt cgtataatgt
     5161 ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcgtgtt cgtgggaatg
     5221 gttatgttct gtcataagat tacctctttt ctttttatta ttaaaataat tgcttacctg
     5281 agccgtctaa ataattttgt aaaatcatca cagctgctaa tttatcgatg actttttttc
     5341 gtttcgctct tgaagtattt gcctgctcta ctagcatacg ttctgcctga acagtggtta
     5401 gtcgttcatc ttgataatca accggcaagg agaatagttc tttgatcttc tctccatatg
     5461 acatggaagc ttctgccctt ggtccgatgg aattgttcat attcttaggt aatccaacta
     5521 caaaacggtc tacttcgtac tgcgccacta attccttcaa ccgatcaaaa ccaaactctt
     5581 cttctgcctc attgattcga atgatttcta ctccttgagc tgtccagcca aagggatcgc
     5641 tcacagcaac gcctactgtc ttagaaccga catccaagcc cattactctc ataaattcac
     5701 tccatgattt gagaggtagt ctttgacaat tacttccatg atttcatcgc gttcgtgacg
     5761 gcgaatcaaa tttcgtgcat cacgataacg aggaatgtag gccgggtctc cagaaagcaa
     5821 gtagccgaca atctgattga ttggattgta gcctttttct tccaaagccc gataaacaat
     5881 cgctaatgtt tcgctcactt cttttttgcg actatcgtca aaaccaaaac gtacggtttc
     5941 atctgtaaaa cccatgattc tacacctctt tcccgcaagg taattccagc ttaccttcta
     6001 cacccttatt ttactcgaag aaagggaaaa ctacaaacaa atattccaaa cttctgaata
     6061 aattaagaaa cacgatttgt ctgatttttc cttttttctg tggctttttc aaaagccctc
     6121 tttccataaa gtatacgcta tatttctttg aaagtatgtt tttacacaat tttttcataa
     6181 agttcataaa aaattcgaat tgctgctact aatttttcgt tttcgttgct tgccctgtct
     6241 cataattgat ggtgtagcca tcttgaacat tgtagataaa gacattgaat tcaatttttt
     6301 tgtcttcaat gctttctgct tccaactgca cacctcttgc taccagttca tttccctcga
     6361 aatgaggagt gacacgatag cgtacatgat gatttgtttc cttgatatat gccgcaatat
     6421 cattttcgtg ggcaagcatg tacggagcat tcaacgaacg tgtccctgtc attaagtttt
     6481 taatgttatt attttgacca gtcaactgga aaccaatcaa atggctgcgg ttgtataacc
     6541 attgtccgtc acttaatttc ttttgtttcc aacctgtcgg atcaatatac aaaggttctc
     6601 tcttttcagt aggaaacaag tcttttccca acatcgcatt ggcttgtcct actctgttca
     6661 atttgtctat atttgaatag ctttcccatg tccctttgga aagactaaga tcctcttttg
     6721 taaactccgg ctgattccca ttgatttcta tttcttgatg ttttccatca tattctggca
     6781 catccgctac ttgagaagct tgcggtgtgg aatcaaaaat ggttgtaatt tgttggaaga
     6841 tatctgaatc tttttgtgtg gtggaaatac cgagtgcccc tgcaatcaca gcagcgacga
     6901 tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac taacagttcc
     6961 ctaaatctta acatatgttc ccctataaat aaatactgga aagaaataga aacagtaaaa
     7021 aaataaaacc agtttttaga cggcttaaga atacaaaaaa agagacggat tcatcgtctc
     7081 tactcaatca caaaacacta tttacaaggt atttattatc attcaaaaaa tttttcttaa
     7141 tactcaaaac taaacggctt ctgtacgacc tcttggtaaa cagtgttcaa aagcttgcat
     7201 ctgcggtaat aagtccaacc aagtcaaaaa cataagaagc ttttttgctt gactattctt
     7261 attacttgat gcagggcagc tttttcacag cctcttttaa gcgggtgacg ggaatcgaac
     7321 ccgcgacatc agcttgggaa gctgggattt taccactaaa ctacacccgc gatacaaaaa
     7381 cagtatacgc ctctttcact ccttcctcaa gtttttttat tctttacgct tccgatatgt
     7441 tttgtagtaa aataaaacta atcaatcaat tattggagaa gatgctatga cgggaaaaga
     7501 attagatctg ttgctggaca cttgtttact cgctgggaaa atcatgatgg aaagcaatgc
     7561 ggagatgtat cgggtagaag atacgatgag tcgaattgct ttagcttctg gaaatttccg
     7621 tttggtcagc tatgtcactc aaaccggtct ctttattggt cttgatcgta cctcgaccat
     7681 ccgaatggaa caaattacca accgttcgat taatttagaa aaagtagtaa aagtaaatga
     7741 tctttcacgt aaatatgttg cttcagagct aacattagaa gaactttact gcgaactgca
     7801 agcaatcgac cgagaccgtt ctttctttcc tgtctggttg caaatcgtta gcgcggcagt
     7861 gatcagtgga acaattatgg tgttatttgg cggaaagatg accgatttac ctgtgacttt
     7921 agtcattgga ggattgggat acgcagctta tctatatagt ttgaaatttt ttagaatcaa
     7981 atttttatcg gaatttttat cttctttatt gattggcata gccgctatct acagcgtacg
     8041 ttttggctta ggaagcaatc aggatctaat cattatcgga tgtgtgatgc ctttagttcc
     8101 tggagttcaa attacgaacg ctatccgtga tttgctagct ggacattatg tctcaggagt
     8161 ttctcgcgga acagaagcga tgatgactgc tacgatgatc ggttttgcga ttgcttttat
     8221 tttccagctt ttttattagg aaaggaacac aagtatgggg acgatattta ttcagttttc
     8281 ttttagtttt ttagcgactg cggcatttgc cgttattaca aatgtaccta ggcgttcatt
     8341 gatttgttgt ggtctttctg gtgcttttgg ctggatgatc tactggatct gtgttcaaat
     8401 aggcggatct gctgcttttg gttcattgct tggttcttta ggagtagcat tcatcagcga
     8461 tcttttctct aaacgtttga aaatgcccgt aacgattttc aatattcccg ggatggttcc
     8521 tctagtccct ggtggtttag cttatcaagc ggttcgaaat ctcgttactg aatcctatcg
     8581 agaagcggct cattatgcgg tccaagcaat tatgattgct ggagcaatcg ctttaggtct
     8641 tgtattatca gaagtcttta accataacat ccgtaatttc aaagtaaagc gtgacgcaat
     8701 catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt
     8761 gaaagagacg ttcagccttg agcgatgaca aaatatctca caaacattgc gacaagtaac
     8821 cgcaggagca ataagaagta attatgagta ttaaaaaaag ttttgtaaaa atagcttctc
     8881 acatttttac ataaaattga cttaatactg agaaactgac ttttgaacac cctctatctg
     8941 taaaaaaggg gctgcgacat gatttttgtc acggcccctc ttctttattc aaattttttc
     9001 caaaacgttc gttgataata aacgatccat agagcggaaa gtattagaac taaaccgatc
     9061 acaggaatat tcactaacca aaatggataa aacaaagcca acattccgta taagacgata
     9121 agaataatac tgatgatcaa tgaacccatc atagttgcga ctaaacccaa acttcctgaa
     9181 cctctgttaa ataattggtt aatattggtc cagttcagca gcagcaaccg ataatctcta
     9241 gcgaaataac gtagtgacaa taagtagcag ccgaataaag aaccaaatag caagctaggg
     9301 ataaaaaaca aaggcagctg aaaagacagt ccgcttaata atgcgatccc accagttaaa
     9361 aaactctgca atatccatcc gaatctgaac ttttctttta aatattggtt catagagatt
     9421 ggcaaagaac ggataaacag aaaattttct tgatccaaag aaataagatt gctgataaaa
     9481 gaagtctgat taactgttaa gaaagccaat gcgatcccta ccaggaaaac aacaccgatt
     9541 aacttattat ctaaatcagt taattttatt tcgcctccta gggcaaaagc aagaataaaa
     9601 atgattggag ttaacaagga agaagaaaga acttgcataa tcaaattagg ttcttttact
     9661 aattgagcat tgtaactaaa taaaagctga tgcacatttt ggttcttttt atgggttctt
     9721 tttattttga actcactgtt tgaatcagtc agttgctctg taagctttgg tactatcagc
     9781 tgttttacag caaggaaaaa tcccagagcc gtgatagcta gtattcccca acccaataaa
     9841 ccttgttgag aaagtggctg actggcagca tcatagaatg gcaataatat cgaaatcgga
     9901 tgccgatctg ttaactcatt ttcaatagtc cccgtttgat gattcatcca catgattcca
     9961 gcaaatgcga tcagcatcga gctgccaagc aagatactag aaactgtttt tctatgtttt
    10021 ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac tagccagaca aaaaagaata
    10081 atcaaaaata gagaaaacaa aaggattgag ataatgatag taagcaaaat ccctagatca
    10141 gctcgccatc cggttaaaat aaacatgact agaagcggaa aaacaaaagg aatgatggtc
    10201 aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa
    10261 aatacttgca ggtcatttcc ttcaaaaaag atattataaa tgactgatat actttgagtt
    10321 aaacctaaca taccaaaaag tgccacgtaa taagtaaaga aacctggcaa tttaacaaag
    10381 ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt
    10441 tgagacaaaa gatagatagt taatcgcttt cccttttttc ctttctctct tgctttattc
    10501 gtgagctgtg gattaacata taaaagatta actcgaatta attctaataa ttgtcgctta
    10561 tacatacggt ccatctcctg tcgcttgcct tccagccatt ttcaaataaa tcttttctag
    10621 cgtttcatca ggtgagccat tcaataattc actaactgcc ccctgataaa tcaattcacc
    10681 tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg gtatccaata catgtgtaga
    10741 aaaaatgact gtttttcctt ttgcggcatg agccttcatc atttctttca aatcaaatgc
    10801 tgcttgagga tcaagtcctt gcaaaggttc atccaatacc caaatatctg gatctggcag
    10861 caacgtgccg atcaaaatcg ttttttgacg cattccatgt gaaaaacttg cgattgtttc
    10921 attttgatga ctatacatat caaaaagcgc tgtcaattct gctaaacgct tttctttttt
    10981 ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg
    11041 taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt
    11101 ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac
    11161 taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc
    11221 cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact
    11281 tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac
    11341 ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga
    11401 ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata
    11461 tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta
    11521 gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt
    11581 cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt
    11641 tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa
    11701 tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg ctcattagat
    11761 aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc
    11821 tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag taactttgga
    11881 tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct
    11941 ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca attttacact
    12001 tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat
    12061 cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
    12121 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
    12181 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
    12241 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
    12301 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
    12361 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
    12421 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
    12481 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
    12541 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
    12601 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
    12661 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
    12721 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
    12781 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
    12841 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
    12901 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
    12961 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
    13021 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
    13081 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
    13141 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
    13201 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
    13261 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
    13321 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
    13381 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
    13441 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
    13501 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
    13561 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
    13621 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
    13681 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
    13741 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
    13801 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
    13861 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
    13921 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
    13981 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
    14041 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
    14101 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
    14161 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
    14221 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
    14281 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttatttaa
//
LOCUS       DAFFFQ010000029        28357 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000029
VERSION     DAFFFQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.029"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(27..725)
                     /locus_tag="LQB69_001357"
     CDS             complement(27..725)
                     /locus_tag="LQB69_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL3525531.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="LQB69_001358"
     CDS             complement(718..2070)
                     /locus_tag="LQB69_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL3525532.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="LQB69_001359"
     CDS             complement(2293..3693)
                     /locus_tag="LQB69_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL3525533.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="LQB69_001360"
     CDS             3854..4591
                     /locus_tag="LQB69_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL3525534.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="LQB69_001361"
     CDS             4716..5195
                     /locus_tag="LQB69_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL3525535.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="LQB69_001362"
     CDS             5192..5959
                     /locus_tag="LQB69_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525536.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="LQB69_001363"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="LQB69_001363"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3525537.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="LQB69_001364"
     CDS             complement(6862..7464)
                     /locus_tag="LQB69_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3525538.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="LQB69_001365"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="LQB69_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="LQB69_001366"
     CDS             8145..8729
                     /locus_tag="LQB69_001366"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL3525539.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="LQB69_001367"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="LQB69_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL3525540.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="LQB69_001368"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="LQB69_001368"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL3525541.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="LQB69_001369"
     CDS             10630..10809
                     /locus_tag="LQB69_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525542.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="LQB69_001370"
     CDS             10831..11856
                     /locus_tag="LQB69_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL3525543.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="LQB69_001371"
     CDS             12031..13275
                     /locus_tag="LQB69_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL3525544.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="LQB69_001372"
     CDS             13428..14375
                     /locus_tag="LQB69_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3525545.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="LQB69_001373"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="LQB69_001373"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL3525546.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPGGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="LQB69_001374"
     CDS             complement(15277..15519)
                     /locus_tag="LQB69_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525547.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="LQB69_001375"
     tRNA            15640..15712
                     /locus_tag="LQB69_001375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="LQB69_001376"
     CDS             complement(15831..16469)
                     /locus_tag="LQB69_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3525548.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="LQB69_001377"
     CDS             complement(16592..17224)
                     /locus_tag="LQB69_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL3525549.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="LQB69_001378"
     CDS             17575..17874
                     /locus_tag="LQB69_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3525550.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="LQB69_001379"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="LQB69_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL3525551.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="LQB69_001380"
     CDS             complement(20806..21291)
                     /locus_tag="LQB69_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL3525552.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    21381..21726
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21771..22412
                     /locus_tag="LQB69_001381"
     CDS             21771..22412
                     /locus_tag="LQB69_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3525553.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="LQB69_001382"
     CDS             22431..23066
                     /locus_tag="LQB69_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3525554.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="LQB69_001383"
     CDS             23084..23899
                     /locus_tag="LQB69_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3525555.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            24218..25654
                     /locus_tag="LQB69_001384"
     CDS             24218..25654
                     /locus_tag="LQB69_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3525556.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            25843..28290
                     /locus_tag="LQB69_001385"
     CDS             25843..28290
                     /locus_tag="LQB69_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL3525557.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
BASE COUNT         9796 a         4832 c         5775 g         7954 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gggggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
    24061 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
    24121 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
    24181 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
    24241 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
    24301 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
    24361 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
    24421 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
    24481 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
    24541 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
    24601 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
    24661 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
    24721 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
    24781 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
    24841 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
    24901 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
    24961 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
    25021 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
    25081 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
    25141 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
    25201 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
    25261 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
    25321 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
    25381 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
    25441 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
    25501 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
    25561 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
    25621 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
    25681 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
    25741 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
    25801 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
    25861 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
    25921 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
    25981 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
    26041 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
    26101 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
    26161 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
    26221 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
    26281 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
    26341 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
    26401 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
    26461 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
    26521 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
    26581 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
    26641 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
    26701 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
    26761 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
    26821 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
    26881 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
    26941 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
    27001 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
    27061 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
    27121 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
    27181 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
    27241 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
    27301 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
    27361 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
    27421 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
    27481 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
    27541 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
    27601 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
    27661 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
    27721 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
    27781 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
    27841 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
    27901 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
    27961 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
    28021 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
    28081 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
    28141 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
    28201 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
    28261 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
    28321 taaccaataa aaagacaagg catgatgata ccaaaag
//
LOCUS       DAFFFQ010000030        27993 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000030
VERSION     DAFFFQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.030"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(977..1150)
                     /locus_tag="LQB69_001386"
     CDS             complement(977..1150)
                     /locus_tag="LQB69_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525558.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1153..1551)
                     /locus_tag="LQB69_001387"
     CDS             complement(1153..1551)
                     /locus_tag="LQB69_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525559.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1767..2822
                     /locus_tag="LQB69_001388"
     CDS             1767..2822
                     /locus_tag="LQB69_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBL3525560.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2842..3195
                     /locus_tag="LQB69_001389"
     CDS             2842..3195
                     /locus_tag="LQB69_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525561.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3377..3673)
                     /locus_tag="LQB69_001390"
     CDS             complement(3377..3673)
                     /locus_tag="LQB69_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525562.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3677..4495)
                     /locus_tag="LQB69_001391"
     CDS             complement(3677..4495)
                     /locus_tag="LQB69_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3525563.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4721..4942
                     /locus_tag="LQB69_001392"
     CDS             4721..4942
                     /locus_tag="LQB69_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBL3525564.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4944..5210
                     /locus_tag="LQB69_001393"
     CDS             4944..5210
                     /locus_tag="LQB69_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3525565.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5323..5862
                     /locus_tag="LQB69_001394"
     CDS             5323..5862
                     /locus_tag="LQB69_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525566.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="LQB69_001395"
     CDS             6356..6640
                     /locus_tag="LQB69_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525567.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6914..7168
                     /locus_tag="LQB69_001396"
     CDS             6914..7168
                     /locus_tag="LQB69_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525568.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="LQB69_001397"
     CDS             7250..7435
                     /locus_tag="LQB69_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBL3525569.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="LQB69_001398"
     CDS             7502..7897
                     /locus_tag="LQB69_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL3525570.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            7887..8264
                     /locus_tag="LQB69_001399"
     CDS             7887..8264
                     /locus_tag="LQB69_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525571.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8286..8661
                     /locus_tag="LQB69_001400"
                     /pseudo
     CDS             8286..8661
                     /locus_tag="LQB69_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8967..9365)
                     /locus_tag="LQB69_001401"
     CDS             complement(8967..9365)
                     /locus_tag="LQB69_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL3525572.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9556..10323)
                     /locus_tag="LQB69_001402"
     CDS             complement(9556..10323)
                     /locus_tag="LQB69_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3525573.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(10358..11581)
                     /locus_tag="LQB69_001403"
     CDS             complement(10358..11581)
                     /locus_tag="LQB69_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBL3525574.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12562..13365
                     /locus_tag="LQB69_001404"
     CDS             12562..13365
                     /locus_tag="LQB69_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3525575.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13950..14405
                     /locus_tag="LQB69_001405"
     CDS             13950..14405
                     /locus_tag="LQB69_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525576.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14524..15582
                     /locus_tag="LQB69_001406"
     CDS             14524..15582
                     /locus_tag="LQB69_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBL3525577.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15624..17573
                     /locus_tag="LQB69_001407"
     CDS             15624..17573
                     /locus_tag="LQB69_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL3525578.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17576..21217
                     /locus_tag="LQB69_001408"
     CDS             17576..21217
                     /locus_tag="LQB69_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525579.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21617..21955
                     /locus_tag="LQB69_001409"
     CDS             21617..21955
                     /locus_tag="LQB69_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525580.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            22065..22286
                     /locus_tag="LQB69_001410"
     CDS             22065..22286
                     /locus_tag="LQB69_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525581.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22306..24462
                     /locus_tag="LQB69_001411"
     CDS             22306..24462
                     /locus_tag="LQB69_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525582.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24474..24707
                     /locus_tag="LQB69_001412"
     CDS             24474..24707
                     /locus_tag="LQB69_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3525583.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24697..25563
                     /locus_tag="LQB69_001413"
     CDS             24697..25563
                     /locus_tag="LQB69_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525584.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25578..27548
                     /locus_tag="LQB69_001414"
     CDS             25578..27548
                     /locus_tag="LQB69_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525585.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            27715..27942
                     /locus_tag="LQB69_001415"
     CDS             27715..27942
                     /locus_tag="LQB69_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525586.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
BASE COUNT        10028 a         4253 c         5045 g         8667 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaaatca aaatttaaat gttttaatat tacaaataaa atcatgttat
      541 tgtaaaatgg tataaaaaag ctaaatattc tatttttgtt attttattag caggaaaatt
      601 taaatagttt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaagta tcaacataaa catatacata tacacgttta tgttgattca cttaatttgt
      841 cctttaaaaa gcacttattt taatataatg ttattttata atcttattac aagctgattg
      901 ttaggcgttg tcctttcatt acatttatta acggttattt tattgtttaa tttattaatt
      961 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
     1021 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
     1081 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
     1141 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
     1201 agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga
     1261 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
     1321 tctaataccg tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
     1381 ttcttctaaa gaatcaaaat atttttcgac agcaatcttt gaagctttta ctattgaagc
     1441 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
     1501 aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
     1561 tccttaatat aatttatgtt tttataatat cataataaga taaatttgca agcatttatt
     1621 tacacctttt tatttacact ttattataat taattagact tttatgattg catttttttc
     1681 ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
     1741 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1801 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta
     1861 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1921 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga
     1981 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     2041 gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac
     2101 gtttagaaga tcaatctgat gacgttttaa ccgctaatct agttttaggt ctgccttcat
     2161 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     2221 ttgaagttga cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc
     2281 cgattggaac actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat
     2341 tgatcagtca gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg
     2401 tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt
     2461 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     2521 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg
     2581 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     2641 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     2701 gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     2761 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2821 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2881 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca
     2941 ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag
     3001 aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagcagaa
     3061 gtaaagaata aaccaactga tcataaccct attcaacaag agcatccttc aacggttaca
     3121 tccgatcatt cagcaaagaa agaaaagcca atgctgaaag ggttcaatgc accaagtaac
     3181 aaagacttac tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac
     3241 aaatataaac ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat
     3301 aaacacgtat attaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg
     3361 atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac
     3421 caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc
     3481 tttgcgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta
     3541 gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt
     3601 caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt
     3661 tcacttcaat cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg
     3721 tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa
     3781 tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat
     3841 aattagataa cgttggtacc agttctagga actcacgact tgaattagta ttatgtttga
     3901 tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa
     3961 tcaactgttt tgctagtgca tcaatatctt taccaagctt cagcaaagct tcaaaaccat
     4021 ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta
     4081 gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt
     4141 catagttaac aaccatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt
     4201 gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttacag
     4261 ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt
     4321 tactgaaagc atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca
     4381 gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt
     4441 tacctgttcc acctttttca atgtttccag taatgattga caacttcttt tccatgatta
     4501 aacctccctt attaaccttt tgtaactcca ttatacacta taaacacgtt tatgtatatg
     4561 tttatataca taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata
     4621 tatatgtata tgtttatgtt tatgtatata ttcatatata taactattgc atttgtcata
     4681 caatcgtagt acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg
     4741 tgtatctaaa gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat
     4801 ttctgaattt atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac
     4861 ctacaaggaa ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat
     4921 gctaaaagag ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa
     4981 aagcgctaaa aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa
     5041 atcttgtagg cactgatgat ccaagaaaac atggtaaagg tctaacagct aataaatcag
     5101 gctactggcg atatagggta ggtgcctacc gtataatagc tgatattagc gagcatgaag
     5161 tgacaattct tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct
     5221 gataaggctt gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta
     5281 cctaaaaaag aacaataccc tcttgttatt atacacactg gtgtgtataa tataagtgta
     5341 aacaagctaa ttaactttca aaaggagcga ttttttatgg gatggtatgg tgcttattca
     5401 tcagcaaatg aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta
     5461 gataccaaag taacgaagac ctatggtatt atccttttca aagatgcaga aggaacctac
     5521 gatattgaca gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac
     5581 agtcctgctt actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa
     5641 gaaaaaatgg ttaagcaaca actagataga attaaagaag aaaacaataa accaaacctt
     5701 gatgacttat tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtt
     5761 tatcagtata aactcaaacg tagctttatt tttagactac aagatggatc actcactcgg
     5821 ttcactcgac tatcagcagg agatgttaag gaagtagcct aaaacttaaa tacataaaca
     5881 taaacgtgta tacgtttatg tttatgttta tgtatatgta tatgaatata aacgtattag
     5941 gtacgaacgt ttttaaatgc ttttctgggc tttgatttta gggtagggct aaaacatcca
     6001 ttgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactga taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 accggactag ccgtttcctc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacacta
     6301 gtgtgtataa tgtaattgta aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tagggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tagatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttgact tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg agggtagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgtttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt tttttataag
     8461 tcagattgtt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatctttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataattta tccaaacaca
     8701 ttacaaagca cctaattcga gcttatatca agcaaatttg ctatttattc ctcaatacat
     8761 atctaaaaat tacttgatgg aaaactagat acatggtaag catgaaaaga aaatcaattt
     8821 gagcagatgc agtataatac atagcaattt aaaagctctc agaacgcttc tgagagcttt
     8881 taatatattc tttgataatt tgtctttctt aaatccatac gtcctaaaac aagctgtttt
     8941 tgcttaatat gatcatctga gagacatcaa atcaactttt ctctcaacaa atcattcaaa
     9001 gctttcgaca gattaatatt ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc
     9061 actgattttc gatcgtaatt ttgcttgttt cgagctttag caactttcgt attagctttt
     9121 acgacaaata aattaacatt ctcagatcgc ttgattcttg catttaactc ttgaacaggc
     9181 gtaggcgatg gaatttcttt ttcttgcaac tctaaaagca ccaagtatcc ttccaatact
     9241 tcttgcgcca tctcaaaagc atcttctaaa gtttcaccat gcgtatataa gtctggaaaa
     9301 tctgggaatt taactgcata ttcatttcct ttccattcga atactgcaca aaaaaagtat
     9361 ttcatcgcat tgcacctctc taattaaaaa ataatttttg ctttttagtt tcaaaaaaag
     9421 aatcgcctac caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta
     9481 ataccttgct cccaaggcac tacatatctt agcatacgtc tgaagcatct tcaaacgagt
     9541 aagatatatt ttgcattaac ctaatttttt ttctctataa ggtttttcat caaactcact
     9601 agaaatatta aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc
     9661 aatcttcagc attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc
     9721 aaaattcgat attgttttgt actcattttc tgttttcgta atctcacgaa ttttaactat
     9781 ttcaatcagg ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat
     9841 aagatatttt ttataataca tcgccaaata tttgtataac acaatgctat attttttttg
     9901 aaagcttgct aaatcttgaa aaacaaaata tggatatttc tttttgattg aaagaattag
     9961 gggaattact tcttgcgaga aattgaaagt tataatttgt tcttctaagt cccaatgaat
    10021 ttgatcaata gcagatactg tagcataagt cttatattct ttatctaagt tcgatatttc
    10081 ataaactgct tgtctctgaa gggtgttaac tatatttttt agttctgttt caagattttg
    10141 atcaacatcc atgtatttga ttattgcttt aacaggtaaa actatttccc ctgttttctt
    10201 taatttgtta ggagagatat aactgaacag caattgataa agttcatacg actttaaatc
    10261 cattttcatg tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt
    10321 caaaagtttt tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag
    10381 gcaaataact cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct
    10441 ccttatgagt agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg
    10501 ataaacgatc ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc
    10561 ataagtcggc agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca
    10621 tagctttcaa gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag
    10681 cagggtctct ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga
    10741 actttatgtt tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag
    10801 tgtatttatt cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag
    10861 ctttatattt tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat
    10921 tttttaatct atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca
    10981 gattaaaatc tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa
    11041 ctacttgatc aaatgtatat tgcgtaaaat gatcttctaa gttcgttaat aaaggcataa
    11101 ttgaactact aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta
    11161 gcggtgagat gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga
    11221 cagattgctg atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg
    11281 aactagaaga catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag
    11341 gcggattttt agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat
    11401 ccatttcaac aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt
    11461 ttagattgct ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac
    11521 gattgcctct tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca
    11581 ttgaataagt atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata
    11641 acataactca atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata
    11701 cagcagaaat cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga
    11761 aattataaaa attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc
    11821 acatactttt tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg
    11881 catcgctttt tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca
    11941 ccctaaaatc tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga
    12001 ttaaaatata tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt
    12061 ttttcaatta ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt
    12121 aagatttttt atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat
    12181 ctgttaatat atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg
    12241 ggagagtaag tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag
    12301 tggacagtaa gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat
    12361 cacactagga taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa
    12421 ttagattagt aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac
    12481 aagcagaaaa aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa
    12541 aagaagattg gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat
    12601 gtagatttag tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca
    12661 acagctttgc gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa
    12721 atgaaacgtc aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc
    12781 aaatacgaca gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa
    12841 ctaggtaatt taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt
    12901 caaaaattcc ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt
    12961 gaatttatct taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca
    13021 aaactaagaa gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa
    13081 gagtggcgaa atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata
    13141 ccttcaagtt atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa
    13201 tttttagctc ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc
    13261 tatgcaaaga aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca
    13321 aacttgccaa ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt
    13381 aattttaata caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc
    13441 aagaagtttt tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta
    13501 tacaaatttt aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt
    13561 cttaatttgt tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt
    13621 gatatcagtt ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg
    13681 gacaagtcaa ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg
    13741 tctgataaat ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa
    13801 tagctaaaat aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc
    13861 tgattgattt atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc
    13921 tcattttaaa aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta
    13981 actatatgga gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc
    14041 atgggttacc agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta
    14101 ttaagctgag aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg
    14161 aactaaaagg caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta
    14221 ttggaaaata tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta
    14281 ctccaatggt tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa
    14341 tagcaaaaat cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa
    14401 gataattttt gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga
    14461 acctagaatt accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa
    14521 ctaatggaaa aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg
    14581 ttgcttggtg ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt
    14641 ctaggagtgc tttttttatt aacgggcttg ggtagctaca tttatcaatt aggaaactat
    14701 aattattatc tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca
    14761 ggagcagaga tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta
    14821 aaactcggtt actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac
    14881 ttatatatgg caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg
    14941 ttaagtactg atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat
    15001 caagttttta ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat
    15061 accaaagtca atgtgaatga agcagattca gttacaaaag cactttctca atattctatg
    15121 aatgatgtta ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact
    15181 cctgataata tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga
    15241 agctttctta ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag
    15301 aataatgttg taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa
    15361 attgaagcta caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat
    15421 tcgcaagaaa gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat
    15481 gatacacaaa aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga
    15541 gacatgaaga cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt
    15601 ttagaggaag tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa
    15661 ctgaatttaa tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg
    15721 atgacgaact ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct
    15781 tgttaggtga agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc
    15841 gacaagatgg aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg
    15901 gagatgatgt cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc
    15961 gaattttaaa atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata
    16021 aagttgttag ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt
    16081 tagcaaatga atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata
    16141 tcaaaagaga tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa
    16201 gcgatgttgt cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc
    16261 ctcaggcttc ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac
    16321 aagcaaacaa gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg
    16381 aggatttgat tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc
    16441 aaatatttgg taagtataat attcagctag attctgattt tgatatggaa aaacttcaag
    16501 aggaattaaa gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta
    16561 aacgtatgaa gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc
    16621 tgtttacagg atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac
    16681 tttatggcga tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg
    16741 atgcaatgcg ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta
    16801 ttgtgtgtga tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt
    16861 tagatgaagg taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca
    16921 ctgtttcatt gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg
    16981 attcgttaaa cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat
    17041 atagcactga actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga
    17101 ttggtgaagg tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg
    17161 ctttatccga acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat
    17221 tagtacgaaa acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg
    17281 gtcaaaattt ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag
    17341 aggattctaa atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag
    17401 aagcgattac tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga
    17461 gtgaagccgg agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt
    17521 ccgttgctga aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa
    17581 attcaagaaa tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt
    17641 tattggagga gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc
    17701 tgacaatcaa gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag
    17761 taaaccggca gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga
    17821 agttaatgat agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag
    17881 tgccgatttc ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc
    17941 aggtactcaa ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt
    18001 ctcaggaaat gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat
    18061 tggtagtttt atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga
    18121 actccttgga aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg
    18181 tggatcggat gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg
    18241 attaaataat gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat
    18301 actaggttat caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc
    18361 accagtactt gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc
    18421 aggatcgcta ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat
    18481 accaacaaat gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa
    18541 tcttgctcca tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat
    18601 caagttcgtt gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa
    18661 tactagcagt tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct
    18721 attaaaagaa tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa
    18781 tgtcgccgaa aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt
    18841 aggggatgga aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg
    18901 gtcagcaaat caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca
    18961 taactttgat gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc
    19021 ggtagcttta ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa
    19081 tatcgctcct tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg
    19141 gcgtacagca tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat
    19201 gaacgctgaa tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa
    19261 tgtgaatatc gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac
    19321 aggatcactt gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc
    19381 tctgatcgga ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac
    19441 tgctgtggga ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc
    19501 tgctatgatt ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa
    19561 tttgattaat atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc
    19621 taagttatta tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc
    19681 aatcaagaaa agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa
    19741 agatatggtt gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa
    19801 caatatggga gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac
    19861 accagaagga atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt
    19921 caaagattta gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt
    19981 aggacgtatg gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga
    20041 tagtaaatac ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga
    20101 tgaagatgga aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa
    20161 tattggtatg gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga
    20221 tggaaatctt gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag
    20281 taacggacgt ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga
    20341 tgagtttggt aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt
    20401 cgatgaggat gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga
    20461 actcgaacaa caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga
    20521 gattacttta gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc
    20581 agacagagtg catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct
    20641 taacagcaac tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc
    20701 gctaacaaaa gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc
    20761 taatgttaat caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa
    20821 tcaatcaatt agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc
    20881 tacttcagat aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc
    20941 agccgttgaa tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt
    21001 agcaaaagct ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat
    21061 taaaataggt aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac
    21121 tggtaaagta gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa
    21181 tcaaaaatca actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc
    21241 cttcttttta ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa
    21301 aattatttta aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa
    21361 aaagggctta tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc
    21421 atttttagcc aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa
    21481 atttatgtta ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga
    21541 tcattgacaa gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta
    21601 aaaaggaggt ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga
    21661 tatgaaaact ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc
    21721 aggattcact gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt
    21781 tggggcgatt attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat
    21841 gggtactaaa acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc
    21901 agttgttggt atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg
    21961 aattgaacaa atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac
    22021 gctccaagcg tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag
    22081 aaaaaatgtt agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg
    22141 caaagataat gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga
    22201 ttgaaaaagc cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag
    22261 ataaacagaa aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa
    22321 gatattaccg aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg
    22381 aagccacaat tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct
    22441 aaagcaaagg tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat
    22501 tatatggtaa ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat
    22561 gctattctcg attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct
    22621 gatctaccag aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt
    22681 ggtcaattat tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct
    22741 acagacttct atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt
    22801 gctgagacct tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca
    22861 gatgaaaaag aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa
    22921 gtaagcatag aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa
    22981 aaacccgaat cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct
    23041 gaaagtgatg taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc
    23101 attgctgaca aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac
    23161 gaggagatgc tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg
    23221 aacgctcgca tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag
    23281 caattgatcc tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca
    23341 acctcattaa atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac
    23401 gaagcagaag catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa
    23461 gattatgata tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg
    23521 tatgatgctg agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact
    23581 gaagaaagtc ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg
    23641 aaacaaagcc aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca
    23701 gaaattgaaa aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca
    23761 aagtcagaaa atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc
    23821 aaatattttg cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta
    23881 gaaacaaaac ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag
    23941 gtcaaagatt tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa
    24001 gctttaagag aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg
    24061 aaatcattag cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa
    24121 aagatggcta ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc
    24181 tactttatta gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga
    24241 gtggaaaatt cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca
    24301 acaccaacta cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca
    24361 actaaggctg aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga
    24421 aatcagtaca aaattcaagc tcacttatct gataataact aaggagggct attatgcgag
    24481 aagaaaaaat agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc
    24541 acttacagga agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa
    24601 tgagcaagtc aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg
    24661 tttaccgtaa gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga
    24721 tttaaacata acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa
    24781 aagcatgtca atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt
    24841 gttgcagtcg ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa
    24901 tttatttttg ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat
    24961 atgtttattg atccatctag tggttcattg atttctggtt ctattagact gttcaaatca
    25021 gtagttaatt cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt
    25081 ttaaaagtga aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc
    25141 aatctagatg gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg
    25201 aaatcaatac aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca
    25261 tctcaacctc agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca
    25321 aggagtaaca aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat
    25381 attgatggtg tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg
    25441 gtattggatg aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt
    25501 gtcactcagt tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa
    25561 tagaggtgag agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa
    25621 agagaaaaac taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg
    25681 ttagtgattt aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta
    25741 agcggtgggg aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat
    25801 ttggttggat ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa
    25861 ttgaagcaga taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg
    25921 ggggatcaac gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta
    25981 gcaaaactgt tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa
    26041 ataaaattat tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca
    26101 gcgaacagct aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta
    26161 tgtcatatgg aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga
    26221 atgtctatga gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca
    26281 aaggtttaga tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg
    26341 gtgtagcttt tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca
    26401 aaaacgctac aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat
    26461 tgattgctaa taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat
    26521 ttgattactt tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg
    26581 taatagaaaa ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc
    26641 aaaaggacat tggtgattta tatagacgga atctcgataa actaactcag attttttatt
    26701 tgttagcagg cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta
    26761 acttttatat aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg
    26821 tgaacagtag tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg
    26881 aaacaaaagc caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag
    26941 acacattgag aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga
    27001 aagaaatcgg taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag
    27061 aaaccaatgt tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa
    27121 aaaaagatga tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc
    27181 taattaaaaa aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg
    27241 atacaatcgg ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat
    27301 tcaatacaga tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg
    27361 gaaagatgac cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag
    27421 gagaagatga tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta
    27481 ataaatttca gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta
    27541 ttctttaagt tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat
    27601 atttgagact aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga
    27661 aatttcataa agtgaaacct tattgagtga aataattata gtggaggtta aataatgaaa
    27721 ccaaacataa aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt
    27781 caacatgaag aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat
    27841 tgtaatcagt tgtataaaaa ctttactgat gaagaaaaag cgagagtttt ttttaatgaa
    27901 gtcaaaggta tgagagaaca gtatgaggtg gttgaagcat gaataagtta ccagaaaaga
    27961 accgtgtatt tagattgaaa ggtgatctca gtc
//
LOCUS       DAFFFQ010000031        27633 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000031
VERSION     DAFFFQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.031"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            119..766
                     /locus_tag="LQB69_001416"
     CDS             119..766
                     /locus_tag="LQB69_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL3525587.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            850..1794
                     /locus_tag="LQB69_001417"
     CDS             850..1794
                     /locus_tag="LQB69_001417"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL3525588.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1929..3380
                     /locus_tag="LQB69_001418"
     CDS             1929..3380
                     /locus_tag="LQB69_001418"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL3525589.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3401..4237
                     /locus_tag="LQB69_001419"
     CDS             3401..4237
                     /locus_tag="LQB69_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL3525590.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4237..4896
                     /locus_tag="LQB69_001420"
     CDS             4237..4896
                     /locus_tag="LQB69_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3525591.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            5022..5711
                     /locus_tag="LQB69_001421"
     CDS             5022..5711
                     /locus_tag="LQB69_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525592.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5717..6673
                     /locus_tag="LQB69_001422"
     CDS             5717..6673
                     /locus_tag="LQB69_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL3525593.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6717..7229
                     /locus_tag="LQB69_001423"
     CDS             6717..7229
                     /locus_tag="LQB69_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525594.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7485..8033)
                     /locus_tag="LQB69_001424"
     CDS             complement(7485..8033)
                     /locus_tag="LQB69_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3525595.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8122..9303)
                     /locus_tag="LQB69_001425"
     CDS             complement(8122..9303)
                     /locus_tag="LQB69_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL3525596.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9539..11047)
                     /locus_tag="LQB69_001426"
     CDS             complement(9539..11047)
                     /locus_tag="LQB69_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL3525597.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11206..11397
                     /locus_tag="LQB69_001427"
     CDS             11206..11397
                     /locus_tag="LQB69_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525598.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11600..12517
                     /locus_tag="LQB69_001428"
     CDS             11600..12517
                     /locus_tag="LQB69_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL3525599.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12510..13583
                     /locus_tag="LQB69_001429"
     CDS             12510..13583
                     /locus_tag="LQB69_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525600.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13731..14249
                     /locus_tag="LQB69_001430"
     CDS             13731..14249
                     /locus_tag="LQB69_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525601.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14230..14622
                     /locus_tag="LQB69_001431"
     CDS             14230..14622
                     /locus_tag="LQB69_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL3525602.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14698..15633
                     /locus_tag="LQB69_001432"
     CDS             14698..15633
                     /locus_tag="LQB69_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL3525603.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIDGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15766..16545
                     /locus_tag="LQB69_001433"
     CDS             15766..16545
                     /locus_tag="LQB69_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL3525604.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16538..16855
                     /locus_tag="LQB69_001434"
     CDS             16538..16855
                     /locus_tag="LQB69_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL3525605.1"
                     /translation="MTKLYLLLLVVCLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            17099..18709
                     /locus_tag="LQB69_001435"
     CDS             17099..18709
                     /locus_tag="LQB69_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3525606.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18866..19714
                     /locus_tag="LQB69_001436"
     CDS             18866..19714
                     /locus_tag="LQB69_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3525607.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            19945..20424
                     /locus_tag="LQB69_001437"
     CDS             19945..20424
                     /locus_tag="LQB69_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525608.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            20457..22685
                     /locus_tag="LQB69_001438"
     CDS             20457..22685
                     /locus_tag="LQB69_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3525609.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            22814..24457
                     /locus_tag="LQB69_001439"
     CDS             22814..24457
                     /locus_tag="LQB69_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL3525610.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(24530..25075)
                     /locus_tag="LQB69_001440"
     CDS             complement(24530..25075)
                     /locus_tag="LQB69_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL3525611.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25098..25583)
                     /locus_tag="LQB69_001441"
     CDS             complement(25098..25583)
                     /locus_tag="LQB69_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3525612.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(25573..26385)
                     /locus_tag="LQB69_001442"
     CDS             complement(25573..26385)
                     /locus_tag="LQB69_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL3525613.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(26524..>27633)
                     /locus_tag="LQB69_001443"
     CDS             complement(26524..>27633)
                     /locus_tag="LQB69_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL3525614.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         9049 a         5027 c         5526 g         8031 t
ORIGIN      
        1 tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca tgacagaaaa
       61 ttttttcttc gttatacttg taatagaata ctattttata acaaagggga tcgaatcaat
      121 gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg cttgtcaatc
      181 aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc gctcttcaca
      241 ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa aaagttctag
      301 tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg aattgttcca
      361 agataaatac ccagatactg caattactag tttggaatta gatagtgatt ggggatctta
      421 cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa ttgatgctca
      481 aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac aaaatggtca
      541 aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg aagcagcaaa
      601 aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg ataaagattt
      661 aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat cagtcaagat
      721 caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat ttcttcggaa
      781 aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt gtttgaagga
      841 gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa tcctttgatg
      901 aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc tcattcagaa
      961 gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa tcccgaacca
     1021 cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa cttaggtttt
     1081 tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca aaatcaaccg
     1141 ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct tgagaaaata
     1201 gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa tgtgcctgga
     1261 aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga agtattcagc
     1321 atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc tcattttgat
     1381 cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat caacagtgtg
     1441 ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc aaaagaaggg
     1501 ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt tcgcgctttt
     1561 tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg gacaggacaa
     1621 gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac cgagcttcat
     1681 aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat tatgtcagaa
     1741 aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc atagcaaaga
     1801 gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct gttcattcct
     1861 gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga ggcaaaaagg
     1921 aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa gaatttagtt
     1981 ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat gaattgaatg
     2041 cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt ccccagtcag
     2101 aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa gaaaaaaagg
     2161 gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct catacatcta
     2221 attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc aatgcgccag
     2281 agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga aacccttgga
     2341 accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt tcatcaggaa
     2401 tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc gcttcgtttt
     2461 gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca tatgcttgga
     2521 gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga gacacggagg
     2581 ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta tcgccagttg
     2641 agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt gattcaccga
     2701 tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta gatttccttg
     2761 gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt aaactgatca
     2821 cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg attgaaacag
     2881 gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact ttgttccaat
     2941 atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg gatgaagcag
     3001 caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca acgactgcat
     3061 ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca cagatggaac
     3121 acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg tttgaggaga
     3181 gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca gcaatcagct
     3241 tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg gctgccagag
     3301 gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat cagttccatt
     3361 taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa aaagattgtt
     3421 tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg ccgctaatca
     3481 ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag cgacttctgg
     3541 cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt atgatgtaga
     3601 tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag aatacaacgt
     3661 ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga acgattttgg
     3721 tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat attcatttga
     3781 cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag atcttaaagg
     3841 gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa aacacggagc
     3901 taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg tagcaaatgg
     3961 tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat ctgctttacc
     4021 agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa gtggtttttt
     4081 attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa aagagatgaa
     4141 agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg tttcagtcaa
     4201 accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct aattgatttt
     4261 tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac attagggatt
     4321 ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt tttaggtctt
     4381 ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt tagaggcatt
     4441 cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt aagtgcctta
     4501 acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga aatctatcga
     4561 ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc tggtttttct
     4621 ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc agtaccggca
     4681 ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac aatcacagtg
     4741 ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta tttttctctc
     4801 tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa agtgcaacag
     4861 tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt gctagcgtgg
     4921 ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta aaaccatttc
     4981 atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc ccgctaacca
     5041 tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg gttgtagaag
     5101 aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg acaaaatttg
     5161 atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat tttcgctcta
     5221 cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt aaactatatg
     5281 aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt ccgtacacgg
     5341 ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca gcaaagaaag
     5401 agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta ttcagggaag
     5461 taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat caattgtaca
     5521 aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga tattatgaac
     5581 ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa gttatcgcgc
     5641 ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg cctacagagc
     5701 cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg acctattagc
     5761 attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag tgatcgaacc
     5821 agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa agcaccatcg
     5881 aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc agcatgtgca
     5941 cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa cgccagaaaa
     6001 tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac agcactaccc
     6061 aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt tgataccaga
     6121 tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg actcttttca
     6181 aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta gtgatgacta
     6241 tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg aaggtagccg
     6301 ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt atattgatgc
     6361 ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg ctaaagacca
     6421 agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc gtcaccatca
     6481 tcaactcttg attacttcag gaatgattga attattggct ctttaccagc aacaaaaatt
     6541 ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac ttattagtca
     6601 cctgctagat catccaaaag attggcttaa atattattca aaagttcagg aactatatga
     6661 agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga aactggatgt
     6721 atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt aatgccgcag
     6781 cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa ccaacaaaag
     6841 aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg gaagatcaaa
     6901 ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa atagatggta
     6961 gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta gcagaaagta
     7021 ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt gcagccagca
     7081 aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg gaaaagacca
     7141 tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa cacggcgaaa
     7201 gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa gataaagaca
     7261 ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag aagtggccag
     7321 cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg aattttagcg
     7381 tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg acatagcttt
     7441 tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat caataatcct
     7501 ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac taaaagtgag
     7561 gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc cagtctggct
     7621 gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt ttctttagag
     7681 atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg ccaaaaacca
     7741 tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat ttctgcttta
     7801 ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt tcccttgatc
     7861 atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt ttccccaggt
     7921 cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat agcttctgcg
     7981 agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt cattgtcatt
     8041 tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa gcttttaaaa
     8101 gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac ttaaaaagct
     8161 gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat ttcctttttc
     8221 ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt tggcaaatga
     8281 aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat taccatttgc
     8341 taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga tactaccggc
     8401 atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa agatgtaacc
     8461 taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga tcatggcaat
     8521 caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga aaccatcaag
     8581 aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa tgatcagctc
     8641 atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat aaccgccaaa
     8701 taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca tataagatcc
     8761 gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca aacgtttctc
     8821 agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc ctaaaatagc
     8881 agaagctact ccattataag attccaattt gcccatcccg ttgattttac ttaatcctag
     8941 accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac cgataatagc
     9001 agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc ctttatttgc
     9061 tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat gatcaaacat
     9121 ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata cgaaaccaag
     9181 aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt ttttgttact
     9241 gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta ataaataact
     9301 caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa ataaaatact
     9361 aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt tccattagaa
     9421 aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag tctataaaaa
     9481 aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta aactttttct
     9541 attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg attcctagtt
     9601 ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct tgtgctccct
     9661 caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact gcactgattg
     9721 ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga agtgtctttg
     9781 atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg tcatctgcga
     9841 tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc atcaatgaac
     9901 ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca gcagaaggat
     9961 cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg actaccacgt
    10021 ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga ttaactgctt
    10081 cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga cggatgattt
    10141 cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct acacgattca
    10201 cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt tcaataatac
    10261 gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact ttattgtagg
    10321 catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg gctgcttcat
    10381 aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt tcgaacttgg
    10441 agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag gaagtagcga
    10501 gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt gtttcaatat
    10561 actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact ggatagtctt
    10621 cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg ataaagactt
    10681 tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat aagccataac
    10741 cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca taatcaccac
    10801 ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca gcttgatctt
    10861 tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt acacgtttcg
    10921 catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca tagtggttca
    10981 ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca aaaccaattt
    11041 ttttcactag gttccctcct taagtactaa agcatttttt catttttttg aggattcgtc
    11101 aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca acttgtctag
    11161 atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa aaaaagacag
    11221 aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta tgaaagaatg
    11281 cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga aacgcagcta
    11341 actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag taaataaaaa
    11401 ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact agttgatcga
    11461 aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc cgctcttttt
    11521 tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa aaacagatga
    11581 gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg agccagacga
    11641 actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt tttacgatat
    11701 caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg cttttgaaaa
    11761 agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc gattgaatga
    11821 agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga tgcgtaagat
    11881 ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca tctcgccaac
    11941 gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg gtattgatgc
    12001 aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga aatattccca
    12061 accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg tgatcactgc
    12121 tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac aaatgactga
    12181 tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa ttcaagaatt
    12241 agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt atattgagac
    12301 gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag cagaccagct
    12361 tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg gtagcaaaga
    12421 gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg agaatcctaa
    12481 taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca gaatggacaa
    12541 aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa gaacaaacaa
    12601 gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa atacacgaaa
    12661 aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct tacgaaataa
    12721 ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc gtcagtttga
    12781 ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg atcgaatcag
    12841 attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa ccaatctcca
    12901 cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa ccgttgaatg
    12961 atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga catttttcga
    13021 tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta ccgaccatcg
    13081 atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag acaatggaaa
    13141 tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg gatcgagtat
    13201 tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta gcccatgcaa
    13261 gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca cttcgtttga
    13321 atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt atcgtggcaa
    13381 tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa gtacgattag
    13441 ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc ttgtcgatct
    13501 catctggaga ctacccacga tctgtcattg atttctttat caattacgaa ttcttggtcc
    13561 cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca ttcagataat
    13621 tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct tttctgtatg
    13681 tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga atgtccacaa
    13741 ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg atcgcttttc
    13801 aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta ctttacttaa
    13861 gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt ttttattttg
    13921 gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg agtttgtttt
    13981 caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga gtctgttttt
    14041 ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg ttatatcgtt
    14101 tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg cacagtatag
    14161 gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac aatcattcag
    14221 gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag tatactcatt
    14281 ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga ggaagtaaaa
    14341 acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc ggaaaaagaa
    14401 gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt tgaagtcaaa
    14461 gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa aaatcaactc
    14521 tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga gatcaaattg
    14581 aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata aaaaactgtt
    14641 aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga aaataacatg
    14701 gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc taatattcta
    14761 gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat gctaaacgaa
    14821 agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg tgataaacaa
    14881 gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa atactttttc
    14941 agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt gatggaagct
    15001 tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt tatagcaata
    15061 ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa agaaatcaaa
    15121 gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg acacagtgaa
    15181 atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt ggctttagca
    15241 ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag cggattgctg
    15301 atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta tagtctgatc
    15361 atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt tgaacgtcct
    15421 gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga gattttagtt
    15481 gcagcaaaag cagatatcga tggtaacaaa gagaatgaag cttactcggt catcaatgat
    15541 attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata cattgaaaca
    15601 gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat tgaagcaaaa
    15661 atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga atttatgtta
    15721 caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa caaaaagaaa
    15781 agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag ctatatacca
    15841 gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct cttatcagga
    15901 ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc tatgcttacg
    15961 acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt acgctataca
    16021 ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt tttggctgtc
    16081 ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt ttcaactgac
    16141 ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac tgcatgggcc
    16201 ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc tgaccttgtc
    16261 cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa aagcgcacta
    16321 ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt attattccaa
    16381 aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt tatgctggag
    16441 aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga tgtcaatgag
    16501 ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat atttgctact
    16561 tttagttgtt tgtctgttca tcgtggcata tatcccgcgc ttgttcccga tgctttattt
    16621 tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac cggtggcatt
    16681 atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg atattgcttg
    16741 gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa cacgttcgat
    16801 ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc tttaaactgt
    16861 tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct atccgaactg
    16921 catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa gattagtgtt
    16981 ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt ccttctatga
    17041 ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg gaatttaaat
    17101 gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc tatgtaaagt
    17161 tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc aagatggtat
    17221 gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc tgatgcttgg
    17281 tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa aagaaggaga
    17341 taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg tcatcggtat
    17401 cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca gtccgattgc
    17461 taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga ttgctatccc
    17521 aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc cttatggaac
    17581 ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta cttattactt
    17641 gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag tcgcttcttt
    17701 cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa aagacttctt
    17761 cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc gatctgcctc
    17821 tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca ttactatcgt
    17881 tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc cagagatcgc
    17941 agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata aattgacatt
    18001 gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat taagcacgat
    18061 gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt cgattgcgtt
    18121 attgattctt cttccttcat tgactggaat gtctttatta gctggaccac tatacacatt
    18181 gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc tagcttcttt
    18241 gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc accatcgttt
    18301 tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag taatcggatt
    18361 aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg gtattgtctt
    18421 tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag caaagatcag
    18481 caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat gttttgccgt
    18541 tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa tgatttattg
    18601 tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg gaaaaaatgg
    18661 gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg ttaactaatc
    18721 gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg tttttctatt
    18781 ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt ctggaaaaat
    18841 tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata gttcggcata
    18901 tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc ctttgattat
    18961 ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt atggactttt
    19021 aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag ttttgggttt
    19081 atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc tttcttcagg
    19141 gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg gagtaacggt
    19201 cattccttat gactctaaga tcactagtat gccgatggga cacatggtag aagctgcctt
    19261 agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg atcgaatccg
    19321 agataatacc tacgcttatc tgattgtaga tgatttgaac aatttagtac gaggaggacg
    19381 tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga tcctgacatt
    19441 ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag catttgctcg
    19501 agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca aactttacgt
    19561 gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc agaaaaaata
    19621 tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac atttaggtga
    19681 aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa aacataccaa
    19741 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
    19801 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
    19861 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
    19921 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
    19981 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatac ggatgcaaaa
    20041 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
    20101 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
    20161 attttagatt tagcgatctt agctgcacaa ggaagtttat acttccaaga tgcttatcgt
    20221 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
    20281 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
    20341 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
    20401 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
    20461 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
    20521 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
    20581 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
    20641 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
    20701 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
    20761 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
    20821 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
    20881 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
    20941 attacccttc tgaaatcgtt cgagcgattg aaaaaggata tttattagag attcaagagc
    21001 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
    21061 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
    21121 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
    21181 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
    21241 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
    21301 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
    21361 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
    21421 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
    21481 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
    21541 agcatacaaa aggaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
    21601 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
    21661 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
    21721 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
    21781 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
    21841 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
    21901 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
    21961 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
    22021 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
    22081 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
    22141 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
    22201 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
    22261 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
    22321 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
    22381 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
    22441 tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag
    22501 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
    22561 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
    22621 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
    22681 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
    22741 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
    22801 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
    22861 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
    22921 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
    22981 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
    23041 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
    23101 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
    23161 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
    23221 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
    23281 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
    23341 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
    23401 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
    23461 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
    23521 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
    23581 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
    23641 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaact
    23701 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
    23761 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
    23821 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
    23881 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
    23941 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
    24001 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
    24061 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
    24121 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
    24181 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
    24241 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
    24301 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
    24361 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
    24421 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
    24481 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
    24541 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
    24601 gggttttcaa acgaggtctg acaggtactt ggacatgttt gatatgcata ccgattgatg
    24661 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
    24721 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
    24781 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
    24841 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
    24901 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
    24961 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
    25021 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
    25081 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
    25141 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
    25201 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
    25261 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
    25321 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
    25381 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
    25441 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
    25501 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
    25561 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
    25621 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
    25681 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
    25741 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
    25801 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
    25861 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
    25921 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
    25981 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
    26041 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
    26101 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
    26161 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
    26221 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
    26281 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
    26341 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
    26401 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
    26461 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
    26521 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
    26581 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
    26641 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
    26701 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
    26761 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
    26821 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
    26881 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
    26941 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
    27001 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
    27061 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
    27121 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
    27181 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
    27241 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
    27301 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
    27361 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
    27421 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
    27481 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
    27541 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
    27601 caaagcaggt tgttccatta aataaagaat agg
//
LOCUS       DAFFFQ010000032        27078 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000032
VERSION     DAFFFQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.032"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            244..1248
                     /locus_tag="LQB69_001444"
     CDS             244..1248
                     /locus_tag="LQB69_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525615.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1249..2193
                     /locus_tag="LQB69_001445"
     CDS             1249..2193
                     /locus_tag="LQB69_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525616.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2193..3155
                     /locus_tag="LQB69_001446"
     CDS             2193..3155
                     /locus_tag="LQB69_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525617.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3182..4096
                     /locus_tag="LQB69_001447"
     CDS             3182..4096
                     /locus_tag="LQB69_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525618.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            4122..5903
                     /locus_tag="LQB69_001448"
     CDS             4122..5903
                     /locus_tag="LQB69_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3525619.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6251..6937
                     /locus_tag="LQB69_001449"
     CDS             6251..6937
                     /locus_tag="LQB69_001449"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL3525620.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6957..10538
                     /gene="smc"
                     /locus_tag="LQB69_001450"
     CDS             6957..10538
                     /gene="smc"
                     /locus_tag="LQB69_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL3525621.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10547..11356
                     /locus_tag="LQB69_001451"
     CDS             10547..11356
                     /locus_tag="LQB69_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3525622.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11367..12365
                     /gene="ftsY"
                     /locus_tag="LQB69_001452"
     CDS             11367..12365
                     /gene="ftsY"
                     /locus_tag="LQB69_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL3525623.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12552..14819
                     /gene="gshAB"
                     /locus_tag="LQB69_001453"
     CDS             12552..14819
                     /gene="gshAB"
                     /locus_tag="LQB69_001453"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL3525624.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14908..15360)
                     /locus_tag="LQB69_001454"
     CDS             complement(14908..15360)
                     /locus_tag="LQB69_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL3525625.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKSNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15510..16460
                     /locus_tag="LQB69_001455"
     CDS             15510..16460
                     /locus_tag="LQB69_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL3525626.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16453..17412
                     /locus_tag="LQB69_001456"
     CDS             16453..17412
                     /locus_tag="LQB69_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL3525627.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17409..18164
                     /locus_tag="LQB69_001457"
     CDS             17409..18164
                     /locus_tag="LQB69_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525628.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18187..19140
                     /locus_tag="LQB69_001458"
     CDS             18187..19140
                     /locus_tag="LQB69_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL3525629.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19784..20080)
                     /locus_tag="LQB69_001459"
     CDS             complement(19784..20080)
                     /locus_tag="LQB69_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL3525630.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20435..20791
                     /locus_tag="LQB69_001460"
     CDS             20435..20791
                     /locus_tag="LQB69_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3525631.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20801..21700
                     /locus_tag="LQB69_001461"
     CDS             20801..21700
                     /locus_tag="LQB69_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL3525632.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22217..22948
                     /locus_tag="LQB69_001462"
     CDS             22217..22948
                     /locus_tag="LQB69_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3525633.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            23093..25120
                     /locus_tag="LQB69_001463"
     CDS             23093..25120
                     /locus_tag="LQB69_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL3525634.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25228..26325
                     /locus_tag="LQB69_001464"
     CDS             25228..26325
                     /locus_tag="LQB69_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL3525635.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            26632..>27078
                     /locus_tag="LQB69_001465"
     CDS             26632..>27078
                     /locus_tag="LQB69_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525636.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         9079 a         4683 c         5706 g         7610 t
ORIGIN      
        1 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
       61 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
      121 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
      181 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
      241 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
      301 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
      361 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
      421 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
      481 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
      541 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
      601 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
      661 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
      721 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
      781 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
      841 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
      901 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
      961 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
     1021 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
     1081 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
     1141 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
     1201 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
     1261 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
     1321 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
     1381 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
     1441 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
     1501 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
     1561 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
     1621 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
     1681 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
     1741 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
     1801 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
     1861 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
     1921 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
     1981 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
     2041 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
     2101 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
     2161 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
     2221 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
     2281 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
     2341 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
     2401 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
     2461 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
     2521 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
     2581 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
     2641 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
     2701 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
     2761 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
     2821 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
     2881 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
     2941 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
     3001 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
     3061 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
     3121 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
     3181 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
     3241 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
     3301 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
     3361 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
     3421 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
     3481 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
     3541 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
     3601 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
     3661 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
     3721 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
     3781 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
     3841 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
     3901 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
     3961 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
     4021 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
     4081 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
     4141 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
     4201 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
     4261 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
     4321 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
     4381 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
     4441 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
     4501 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
     4561 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
     4621 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
     4681 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
     4741 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
     4801 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
     4861 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
     4921 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
     4981 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
     5041 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
     5101 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
     5161 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
     5221 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
     5281 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
     5341 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
     5401 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
     5461 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
     5521 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
     5581 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
     5641 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
     5701 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
     5761 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
     5821 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
     5881 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
     5941 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
     6001 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
     6061 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
     6121 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
     6181 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
     6241 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
     6301 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
     6361 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
     6421 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
     6481 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
     6541 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
     6601 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
     6661 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
     6721 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
     6781 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
     6841 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
     6901 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
     6961 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
     7021 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
     7081 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
     7141 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
     7201 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
     7261 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
     7321 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
     7381 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
     7441 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
     7501 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
     7561 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
     7621 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
     7681 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
     7741 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
     7801 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
     7861 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
     7921 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
     7981 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
     8041 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
     8101 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
     8161 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
     8221 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
     8281 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
     8341 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
     8401 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
     8461 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
     8521 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
     8581 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
     8641 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
     8701 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
     8761 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
     8821 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
     8881 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
     8941 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
     9001 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
     9061 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
     9121 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
     9181 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
     9241 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
     9301 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
     9361 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
     9421 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
     9481 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
     9541 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
     9601 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
     9661 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
     9721 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
     9781 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
     9841 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
     9901 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
     9961 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    10021 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    10081 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    10141 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    10201 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    10261 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    10321 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    10381 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    10441 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    10501 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    10561 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    10621 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    10681 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    10741 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    10801 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    10861 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    10921 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    10981 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    11041 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    11101 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    11161 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    11221 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    11281 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    11341 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    11401 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    11461 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    11521 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    11581 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    11641 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    11701 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    11761 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    11821 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    11881 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    11941 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    12001 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    12061 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    12121 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    12181 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    12241 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    12301 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    12361 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    12421 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    12481 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    12541 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    12601 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    12661 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    12721 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    12781 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    12841 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    12901 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    12961 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    13021 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    13081 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    13141 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    13201 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    13261 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    13321 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    13381 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    13441 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    13501 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    13561 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    13621 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    13681 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    13741 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    13801 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    13861 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    13921 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    13981 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    14041 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    14101 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    14161 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    14221 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    14281 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    14341 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    14401 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    14461 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    14521 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    14581 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    14641 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    14701 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    14761 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    14821 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    14881 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    14941 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    15001 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    15061 tgcagtgacg taatcgttga ctacagtgaa acggctattg cttttcgttg ctagctggat
    15121 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    15181 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    15241 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    15301 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    15361 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    15421 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    15481 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    15541 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    15601 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    15661 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    15721 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    15781 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    15841 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    15901 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    15961 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    16021 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    16081 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    16141 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    16201 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    16261 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    16321 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    16381 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    16441 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    16501 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    16561 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    16621 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    16681 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    16741 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    16801 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    16861 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    16921 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    16981 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    17041 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    17101 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    17161 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    17221 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    17281 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    17341 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    17401 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    17461 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    17521 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    17581 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    17641 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    17701 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    17761 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    17821 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    17881 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    17941 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    18001 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    18061 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    18121 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    18181 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    18241 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    18301 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    18361 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    18421 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    18481 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    18541 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    18601 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    18661 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    18721 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    18781 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    18841 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    18901 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    18961 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    19021 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    19081 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    19141 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    19201 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    19261 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    19321 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    19381 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    19441 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    19501 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    19561 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacgatgtt ccatctgctg
    19621 cttctcggca ataagcccac tagactagaa aatatgagaa gctattttct gtccagcgtt
    19681 cttattactc aaagcaaatc gcagttgtca caatctcttc taaacaatgt tcaatcagct
    19741 gaccttcggt attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag
    19801 caagcggttg atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc
    19861 accgttttaa taattgatag aaataactct cttctgcttt gattttatac tcgcttaata
    19921 tcaatccaaa actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg
    19981 ttttatcttc attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg
    20041 tttcttcgat aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt
    20101 cttttatttg ataagtttac tctaccgaag agttgttaat aatccgtgaa caaaaagtaa
    20161 ttctttttcc aatagttgtt aaaggttttt tattttttat agtatttttt gttatttaag
    20221 aaaggaataa ggttaaacaa ataacaggtt gctttttggg tctttttccg ttcaagaaac
    20281 tgaacgaaga aaagggaaag ttgtctcttt tgtgataaga gaaatcataa taaacgaatg
    20341 acttactttc caataaattg agaacagtca gaaccagaca aattagttgc atctgatgaa
    20401 taaagtgatt attctatgac atgaggtgat acagatgaaa ttagaagtga caaaagaagc
    20461 acaagaagta ttgaagaata aattagaagc agacgatcaa cttttattag atatcgaaaa
    20521 cggtgacggt ccatttgcag aaagccaagt cagttgccaa ttggatacgg catttcgatt
    20581 gatcattgtc aaaaaagata cacaaacaga tctaagtctt tattctgtag tggctgatac
    20641 accagttgga ccaatcaaaa tcaaagacag tgcaatactt tacatggatg atccatccac
    20701 acttgcttta gaaccaactt ataatagttt acaattaaaa ggaccaagcg gcttgagaaa
    20761 aggcaacttg caagttgttc gaaaagacta gagggataaa atgggaaaat tggcgatcat
    20821 cagcgaccta catgtggaca tcaataaatt cggcgaatca gagctgatgc agctttggca
    20881 agttttgcaa aagcacaaga tcactcggct tcatctagcc ggagatacgg ctaataaagt
    20941 agaccgttgc ctagctgttg tcgaattttt cgtcaataaa ggattatcta cgacatttca
    21001 tttcggcaat cacgagttgg cagatgtgcc caatgaacag atgatggacc attttccaga
    21061 cccacatttt ttaaatgaac ggtatattgc tttaaatgag cagaccgttc ttttagggat
    21121 gaatggctgg tacgactatc agttttccga attgaaagat tacgaggcaa tcgtacgaat
    21181 gaagcgtttg tattggtatg atcggttcat ccagcgtggg aaaacagatc tcgaggtcaa
    21241 tgcagaaatg cttgcagctg caaaaaattt gctagaccag ctggaagcaa aaaaacttga
    21301 ggtgatttta tccactcatt tcgttcctaa acgggcattt atcatgtatc aatctgctcc
    21361 ttatgagcgt tggaacaaat taaatgcatt tttaggatca gaatcattcg gagaattact
    21421 tgaccgttat tcaaatatca aacaagtagt ttttggccat acgcatcgtc gctttgaaga
    21481 taaatccatc ggtcacacga tttacagctg cagacctttt ggttattatt atgaatggca
    21541 gctgacccga gattttgtct tgaaagaaca gttgctagac agctatgatc ctatgaaatt
    21601 acgtgtcctc ttgcgtgccc atcacccagc ttttcaggcc tatcaaacac aacatttgca
    21661 agaggaattt gaacaagcga tgacaatcgt tccgtattga gacagcatta gtaatgtgta
    21721 aaagaaaata ccttgtaaaa cataaggata agtaagagca gtagatccat ttatcgtgga
    21781 tatgctgctt tttttgcgga aaataatcag cgaatgttta tttgatcgga tgatttgtaa
    21841 ctcgcagagt actagatgca agcgtgtatt ctttttttga acgataatca caaaaaaaaa
    21901 tataatatac attttttatt taattataat taatattgat tgaagtctag gcagtcaagt
    21961 cacattttga tagataggag ggggacttac gaattatttt aaaggctatg acagctgtat
    22021 ttctagattg tgtttgcatc aagttgcaat aaaaactaga aatcaaatcg ggctgataat
    22081 catttataac taatcgatac ataggaccaa atataggtct atgttctttt atttcataag
    22141 tatcgtaaaa cagaattttg ctatggatta atccaatcga ctgatatgtt tagtaaataa
    22201 agaaagagga atgtcaatga tgaaaaaaga aatcgtatta acagcagcaa tgatgttatt
    22261 cagcatggta gcaagtacga cttttgtttc tgcaactgaa gtatacccaa aggaatataa
    22321 cacagaagga acgattacat ttgaagcagg agatgagggt gtaactcctc ctgttgatcc
    22381 agagaatcct gatcccaata aacctgtcga tccatcagat cctccaagcc ctggaacagg
    22441 aggggcatta tcgattgatt atggatcaaa attcaaattt gggactcaaa aaatttctac
    22501 tgcggataaa acgtattatg cagctgctga tgtgatgaac gatgggtcta gaaaaccgac
    22561 atatgtccaa gtgacagata ggagaagtac cttatctggg tggaaattaa gtgtctctca
    22621 accagaacag tttaaaactg ccagtgggga tgaactagtt ggagcacagc tgaaatttac
    22681 gaaagggcag gctgtttctt tggttgatcc aacatatacg ccgcaaacgg tgaattctga
    22741 attaacgttg acaccgggag gaaacaatac gttagcaatt aatgctaaat caggaacagg
    22801 tgtggggact tgggtctatc gatttggtgc aaatgaaaat gaaaatcaag atgcggtaca
    22861 gctttcggta cccggtaaat ctgtcaagtt agcgcaacaa tattccacta aattagtttg
    22921 gacattagaa gatacaccta acaactagtt tactgaaaag aactgttaaa aataaatacg
    22981 tagtcatatt gtcttgcgag gtaatcgagc aaaaattgtc aaaggctagg ctaaatttgt
    23041 ttggatacga atagttcatc taggaattta acgatcagga ggaaaagata tattgaaaaa
    23101 gaggaggatc gtgctcattt taagcagtct cttagtgact aatatctttc cgatcatagg
    23161 gtactctttg gaattgaatc agttggagca tggaaataga agagacgaag aaagcaaact
    23221 cacttcgaca ttcgacagtt taatagaatc caaccagaca caagaaagca gtgaggattt
    23281 aagtatttcg agtagtggag agaccactga caacgtagaa gctgagggtt cggacaaaca
    23341 aggactagaa gatgcaccaa tttctgcggt tgagctgcct gacgatccca atattatttc
    23401 tatcgataaa atttttcaaa cacctatcgg tactagcacg tccatattgg aaaatgggaa
    23461 gttactgcag ttgaacccag cagaaaggtc tcaaagagga gctatttggt caaataagaa
    23521 gattagttta ctctcggatt ttacatttaa atcttattta tacttaggaa atcaacgaga
    23581 tagagcggga gatggcatga cctttacatt gactggcgat cctagaatgg ctagtaatcc
    23641 cagtcaagtt attggatcgc caggtatggg aattggctca tattctactc gttctggcca
    23701 accttacatt gcgaatgcgc tctcgattga gtttgatacg tataaaaata gtggttcttc
    23761 tgatcgaatg gatcgagaga taactgctga taatagaggg catggacatg tcgcttttgt
    23821 cacgcctaaa ccaaataata ataattattc tggagagcat actggcgtga cggtagctcc
    23881 tacatttctt tctaatggca catggcgctt gttaataatt aaatgggacg cggagcaaag
    23941 aagtcttact tatgatttgg aaggtgtcgg tcgctcttct cggattatac ctgatctcat
    24001 taggcagttt ggttctacag aagtaaattg ggggtttact tcttcaacag gtactttttt
    24061 tcaagaaaat gctgtcgctg tcacgcgact tccatcgaat gtcacatcaa cagctgatgt
    24121 aaaagtcaac gatagtgatt atggggccat cgctgaagct ggaggaaacg ataagataac
    24181 tatcagaaat acattgaagg tcgaggggga ttttcttgaa tataagaaaa tgcagactag
    24241 tatcgacctc ccacaagaat taactattcc cgagcatatc ttgatcaatg gaaatcaaat
    24301 tgatgccaaa gatatcttga taaccggaaa tcgagtcacg ctaaatctga cagattattt
    24361 tattgctgga gatacgttaa taatcgaatt ggagacaact ttgcaggaag taattcctga
    24421 aaaagtactc actatgcatt ttgaatacct tgaaaacggg aatttgattg agcaatcgaa
    24481 tgaaataaac ctaaagatcg caaagctgaa ggagatagat atccatgttt attataaaga
    24541 taaagacagt cagattgata ttgctgaaat gaaaacaatc actgggaaaa taggagagaa
    24601 gtataaagaa actcctattg atattgaagg gtatgttttt gactcggatt cagcgaatac
    24661 agaaggcatc ttctccgaaa atacagagga tatctatttt tattacagaa aaggcaaact
    24721 ttatctctca actgctcctc aatatgtcaa ttttggaaaa cataaaatat ccagtagacc
    24781 attaagtgaa ttcggacagt tttcaggtgg gttgaaaatt atagatgaac gggcttttgg
    24841 aagttggaaa ttacaattga aacaagagaa agttcttaca aatggagatt ttgagcttcc
    24901 agaagctctt tcctttgtta cctctgaaaa tacaacggtt atagggacat ccgctgttac
    24961 gattttcgaa agtgatcaaa agggtgaaag cgatttgtcc tctcttttag attctacagc
    25021 tcatcgtgga atcagaatag atattcctgt agaaaaccaa agaatcggaa cctttgaggg
    25081 gaaactatct tggatacttg cagatgtccc aggaaattaa caggaagaga agaatataac
    25141 aaaattattg agtttcaaga agggcacagg taccataggc tcttgtgtcc cacagagaca
    25201 agattttgtt atagtcttct cttacatatg attaaaggag cgattttctt gagaccaaaa
    25261 caacaattat ttataacact cctagttatt ttaggggcac tttttaacaa tatatcagtg
    25321 tcagctagtg catttaattt ttcagtgacg ccgataactt cagagaatca aatcgacaag
    25381 agaaaaactt acttcgatct tcaacttgat ccggatcagg aagtagaagt aaaagcggaa
    25441 ttgagaaatg atacagaaaa agaagtgaaa atcgatattt ctgtgaatag cgcaaccacc
    25501 aattcaaatg tgatggttga atacggaaaa aacgagattg aaaaagacat gagcctgatc
    25561 tttgatctgg tagattatgt gagctatcca cagatagtga cgctaaaacc taaaagtgtc
    25621 caaaccgttg cttttcatgt caggatgccg aatgagcgat ttgatggtgt attggctggt
    25681 gggatcacat tccaagaaat ccagacggaa aaggaccaaa cagaaactaa ggatcaaagc
    25741 ttatcgattg agaatgaata cacctatatt attgctttgt tgatgcaaca gacactgaac
    25801 gaagttgctc cgaaccttac tcttcatgaa gttaaaccag atcaaatcaa tgcaagaaat
    25861 gtcatcttag caaacgttca aaatgatcaa aaaacctata tcaatcaagt agttattgaa
    25921 acaaaaatta caaaaaaagg acattctgaa gttttatatc aagaggaaaa agaaggattg
    25981 cagattgcgc cgaatacaaa tttttctttt ccaactgctt taaatggtca accgctaacc
    26041 ccaggtgaat atcaccttac aatgactgtt ttaggaaatg aaaatgaaaa tggaaaattt
    26101 tcgagaaaaa aaggaaatac tacgatcaac tataccaatc aatgggtatt tgaaaaagat
    26161 tttacaatag acggaaaagt agccaaggaa ttgaacacga cggatgttac gattaaaaaa
    26221 gacaataatt ggatttatat cttagtagct tcacttttct tattgtttat tttattatgc
    26281 atacttttgt ttgttttgag aaaaaaagaa gagaaagaaa agtaaggtta agataacaaa
    26341 tagtaaattt ttattattgt aggatggttg ggctcttttt ataacaatat aaaatatacc
    26401 attttattaa taaaaatgca tactagtaaa tgatataatt ctaataacag gtttgatcgc
    26461 gagaaaggcc aaataaaacg ctctccaaaa tgttaaaaaa aagacaggac gatgtatatt
    26521 atacaaacaa gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag
    26581 ctagttggtg aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat
    26641 gagttttttt taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca
    26701 agctcaaatt tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt
    26761 ctcttgaaaa aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa
    26821 aatttgcgag taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct
    26881 agcaaagctc tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct
    26941 ttctttcagg aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat
    27001 tctgtggctt ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt
    27061 tttgaaaaga gaaagttg
//
LOCUS       DAFFFQ010000033        26127 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000033
VERSION     DAFFFQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.033"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            12..278
                     /locus_tag="LQB69_001466"
     CDS             12..278
                     /locus_tag="LQB69_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525637.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            291..449
                     /locus_tag="LQB69_001467"
     CDS             291..449
                     /locus_tag="LQB69_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525638.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(446..676)
                     /locus_tag="LQB69_001468"
     CDS             complement(446..676)
                     /locus_tag="LQB69_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3525639.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            748..903
                     /locus_tag="LQB69_001469"
     CDS             748..903
                     /locus_tag="LQB69_001469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525640.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            1060..1215
                     /locus_tag="LQB69_001470"
     CDS             1060..1215
                     /locus_tag="LQB69_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525641.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(1212..1436)
                     /locus_tag="LQB69_001471"
     CDS             complement(1212..1436)
                     /locus_tag="LQB69_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3525642.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1703..2038
                     /locus_tag="LQB69_001472"
     CDS             1703..2038
                     /locus_tag="LQB69_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525643.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2271..3212
                     /locus_tag="LQB69_001473"
     CDS             2271..3212
                     /locus_tag="LQB69_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL3525644.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            3214..4104
                     /locus_tag="LQB69_001474"
     CDS             3214..4104
                     /locus_tag="LQB69_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL3525645.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4147..4947
                     /locus_tag="LQB69_001475"
     CDS             4147..4947
                     /locus_tag="LQB69_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3525646.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            4962..5813
                     /locus_tag="LQB69_001476"
     CDS             4962..5813
                     /locus_tag="LQB69_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3525647.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            5810..6127
                     /locus_tag="LQB69_001477"
     CDS             5810..6127
                     /locus_tag="LQB69_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525648.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            6120..6302
                     /locus_tag="LQB69_001478"
     CDS             6120..6302
                     /locus_tag="LQB69_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525649.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            6304..6774
                     /locus_tag="LQB69_001479"
     CDS             6304..6774
                     /locus_tag="LQB69_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525650.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            6771..7313
                     /locus_tag="LQB69_001480"
     CDS             6771..7313
                     /locus_tag="LQB69_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL3525651.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            7310..7693
                     /locus_tag="LQB69_001481"
     CDS             7310..7693
                     /locus_tag="LQB69_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525652.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            7690..7920
                     /locus_tag="LQB69_001482"
     CDS             7690..7920
                     /locus_tag="LQB69_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525653.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            7927..8130
                     /locus_tag="LQB69_001483"
     CDS             7927..8130
                     /locus_tag="LQB69_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL3525654.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            8127..8423
                     /locus_tag="LQB69_001484"
     CDS             8127..8423
                     /locus_tag="LQB69_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525655.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            8500..8913
                     /locus_tag="LQB69_001485"
     CDS             8500..8913
                     /locus_tag="LQB69_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL3525656.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            8926..9312
                     /locus_tag="LQB69_001486"
     CDS             8926..9312
                     /locus_tag="LQB69_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525657.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            9804..9876
                     /locus_tag="LQB69_001487"
     tRNA            9804..9876
                     /locus_tag="LQB69_001487"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9837..9839,aa:Lys,seq:ttt)
     gene            10262..10522
                     /locus_tag="LQB69_001488"
     CDS             10262..10522
                     /locus_tag="LQB69_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525658.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            10565..10738
                     /locus_tag="LQB69_001489"
     CDS             10565..10738
                     /locus_tag="LQB69_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525659.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            11127..11960
                     /locus_tag="LQB69_001490"
     CDS             11127..11960
                     /locus_tag="LQB69_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBL3525660.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11953..13368
                     /locus_tag="LQB69_001491"
     CDS             11953..13368
                     /locus_tag="LQB69_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL3525661.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13380..14909
                     /locus_tag="LQB69_001492"
     CDS             13380..14909
                     /locus_tag="LQB69_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL3525662.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14827..15879
                     /locus_tag="LQB69_001493"
     CDS             14827..15879
                     /locus_tag="LQB69_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL3525663.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15881..16198
                     /locus_tag="LQB69_001494"
     CDS             15881..16198
                     /locus_tag="LQB69_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525664.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16325..16948
                     /locus_tag="LQB69_001495"
     CDS             16325..16948
                     /locus_tag="LQB69_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL3525665.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16963..17853
                     /locus_tag="LQB69_001496"
     CDS             16963..17853
                     /locus_tag="LQB69_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL3525666.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17876..18091
                     /locus_tag="LQB69_001497"
     CDS             17876..18091
                     /locus_tag="LQB69_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525667.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18103..18444
                     /locus_tag="LQB69_001498"
     CDS             18103..18444
                     /locus_tag="LQB69_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525668.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18444..18815
                     /locus_tag="LQB69_001499"
     CDS             18444..18815
                     /locus_tag="LQB69_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525669.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18808..19203
                     /locus_tag="LQB69_001500"
     CDS             18808..19203
                     /locus_tag="LQB69_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL3525670.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19205..19582
                     /locus_tag="LQB69_001501"
     CDS             19205..19582
                     /locus_tag="LQB69_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525671.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19583..20191
                     /locus_tag="LQB69_001502"
     CDS             19583..20191
                     /locus_tag="LQB69_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525672.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20191..20532
                     /locus_tag="LQB69_001503"
     CDS             20191..20532
                     /locus_tag="LQB69_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525673.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20774..23563
                     /locus_tag="LQB69_001504"
     CDS             20774..23563
                     /locus_tag="LQB69_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL3525674.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            23553..24293
                     /locus_tag="LQB69_001505"
     CDS             23553..24293
                     /locus_tag="LQB69_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525675.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            24290..>26127
                     /locus_tag="LQB69_001506"
     CDS             24290..>26127
                     /locus_tag="LQB69_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL3525676.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         9485 a         3736 c         5519 g         7387 t
ORIGIN      
        1 gcgaataaaa tatggatacc gcgaagaaag aaaaatatac attgagacaa ttaagagctt
       61 taaagggtta ttcaaaagaa gaactgtcaa ggaaaagcaa agtgacatca agaacaatat
      121 ttatttacga aaacgatgta gataaaatga ggaacggaaa gtacgccact ctagaaaaaa
      181 ttgctaaggc attgggtgtt agagtttccg atattttttt agaccctgat tcggaaaaac
      241 cgaaatcaat aacaggtgaa acaaaacaag tcagctagga ggtgtgaata gtggaagaga
      301 agaatataaa agtaccgact cttgaagaat atacaaaaat tgttgtcgaa acagacgagc
      361 agaacccgaa aacaatcgca gttattacgg cgaatgatat tgaatcatgc gaaggttttc
      421 gagttcgaat gacccctaga tatgattaat gttctttatc acgtggaggt ataggatcgt
      481 ttccaaaact atcttttgat tgtatttgtc cattcttttt ttggacaata aactctgatt
      541 gctgtttttt agaaatcttt ttaccatact caacagcatc tgatttatta tcaaatagtt
      601 ttgtatcttc ggaattacct tcaccacgta cagcccattt gccatttcta ggagataccc
      661 attgattttt acccataaca attcacctcg ctttcaagtt aattctatta agaggtgaaa
      721 agaaaacaaa ctgatggagg tgagattgtg agtaacaagc caaaaatcac tttgaatggc
      781 acacctaatc ttcaaagatt agcagatgaa atggctagaa tacgttctag acattgggga
      841 gttactgtgg tggcaaccat aattgaacaa aaagaaaagc ccacacagcc ggcaagcaag
      901 tagtgggcac catagaacaa taatattata tcacacactc gccacttccg aaaagaggag
      961 ttgctgctct cctttcaagt ttgccaaagt ccacgtttct tcattttggc aactcctctt
     1021 cttggaggtg gtgacggcga gagacgaagg agggatgaca tgaaggaaga atcaaaaata
     1081 gggattttga atgagaacaa ttacacaaga ttaattattg aaacagaaga aggggaaaaa
     1141 gtagcagaag ttacattgac cgatgcaact cctgctgacg gttatcgtat tcgattaact
     1201 cctaaatatg attaaccttt tggtgggtgt ggatcgtttc cgtgactatc acggctttga
     1261 atttttccat ctctgccatg aataaatagc tcagatcctt gatttttaga aatctctcta
     1321 gcgattttta ctgcttctga ttttgtatta gtatgtgcag tcgctttaga atttccagca
     1381 cctttgacat tccaaccacc attggaatta ggtacaacat gttgattttt tcccatagtt
     1441 ttcacctcca tttctataga aatattttat caaatagaaa tggagaaatt caataaacga
     1501 aaggagggat caaatgacat tacaagagca tcaacacttg atgcgaaaac taaatcagga
     1561 ataccacgag gaaatgaaac ctagattgct aggggaacaa tggcgtgaac gccagaagaa
     1621 gtggttagaa ataaaaaagt gactcagccg tccaaagcaa tgagtcacaa accaaaaatt
     1681 atacctaagg agattttatc acatgaataa gaaaattgaa aatcttatcg aagaattgaa
     1741 acgtgaatgc caaaagcaag gtgtttctat tatttgtaca gcccaaaaag aaggggaact
     1801 taaaagtctc gtttatggtg agacaacaga aattttactt tgtcttgcta tgcaagaaga
     1861 acatttagat gaaaatctcc cattgtcagc acatattatg cgtagaattg ctgtggatgc
     1921 ttacgaacaa gcgaaaaatg aagaagaaaa tcaaccttca aatcatactt ttgttattaa
     1981 taacaaggaa gatttagctg atgtgatgac tcgtattctc aagggggaat ttcaatgatg
     2041 aatgaaaaaa caattgatct actcattatt ttatttttca cgttagtgat tgtagcgaaa
     2101 agttatgtaa tctttggctt gttattcgca attaccatgc ttgtgctttg cgtgttagca
     2161 agcagaaaag aggttaaaca tgaacgagtt agcaaatctt gacaactatt taactgatcc
     2221 tgattatgct aaaccaccat atgaagcgcc aattgatgag gaggatgaag atgagtaaat
     2281 ctaccttaga aatgagccat caagaatggc tcgaagaccg caaaagaggt attggtggct
     2341 cagatgttgc aaccgtactt ggattaaaca aatacaaatc tccttatcaa ttgtggcttg
     2401 agaaaacggg tcaaattgaa ttgaaagatt tagaaagcga accagcttat tggggtaatg
     2461 ttttagaaga agtggttgct aaagagtttc aggaacgtac aggcaaaaaa gtacgcagaa
     2521 gaaaccaagt atttgaacat ccattacatc catttttaag agcaaatatt gatcgggaag
     2581 tagtgggaga aaatgccatt ctggaatgta aaacagccaa tcaatttctc ggaaaagagt
     2641 gggaaggtga agaggtaccg cttagctatc tctgtcaagt tcagcattac atgaacgttt
     2701 taaacaaaga ctattgttat atcgctgttt tgatcggtgg acaaaaattc atttggaagc
     2761 ggattgaacg agatcaagag ctgatcgata caatcactga acaattagta gagttttggg
     2821 aaacgaacgt tcttggaggt atcgagcctg taattgacgg aagtggagcg actgctgact
     2881 tcttaaaaga aaaatatgca gatgtagaag aaaatcaaac agctctacca tcacgttttg
     2941 atgaacttat cgagcaaaaa agagaactca agcggacgaa aaaagaaatt gaatcatcta
     3001 ttcgtcaagt agacaacgag atcatcagtg agctaggtaa acgtgaagcc agtatcggta
     3061 tcacacaaag aaacattatc agctggaaac ttgttcgtac aagacggatg aacacgaaga
     3121 aactagcaga gaaatatcca gatgtcgcaa atgatgaaga gatttataac gttactgaat
     3181 caagaaggct aaccgaaaag gagatcaaat aatatggcaa caaatgaatc gttaaaaaat
     3241 caattggcag aaaagccaca gaaacaagtt gcaccaggac agttagggct taaagctcta
     3301 atgaatacac caacaatgag aaagaaattt gaagaagtac ttcatgacaa tgctaatgct
     3361 tttatgtcga atgttatgac tcttgtatct aatgacagtt atcttgcaga tagtgaaccg
     3421 atgtctatca tgagtggtgc gttaactgct gcaacattaa atcttgggct agataagaat
     3481 ttaggttatg catatttagt tccattcaat agtaaaaaca agcaaacagg aaaatgggaa
     3541 aagaaagctc aattcatgct tggctataaa ggatatatcc aattagccca acgatcaggt
     3601 aaatacaaag cattaaatgt gatcgaagtt tacgaaggag aactaaaaag ctggaaccga
     3661 ctgacagaag agtttgagtt tgatccaaat ggtagaacgt ctgatgaagt cattggatat
     3721 gttggctatt tcgagttact gaatggattc aaaaaaactg tctattggac caaacaagaa
     3781 atcgaagctc atcggattgc taacaataaa gatcgcgata agacaaagtt aagtggtgtg
     3841 tgggcatctg attacaatgc aatggcacga aaaactgttt tgagaaatct tctttctaaa
     3901 tggggaatct tgtccatcga aatgcaagaa gccaccacat cggatgagag agtccaaagg
     3961 gttcaagaag acggcagcat tattgctgaa acagaagttg aagaagatat tcctgaaaga
     4021 aaagaagcag aggttatatc tgaagaaaat gaagatgtac aaactggatt atttgatgca
     4081 tctaatccgc cgttaaacaa ataatgaggg agctttctcc cttgcttttc tagaggagaa
     4141 atacgaatga acacaggata tgtcaaatta catcgaaaag tgatgaattc attcgtttgg
     4201 accaatcctt atatgtataa attatggagc ttgtgtttga tgaaagcgag tcatgagaat
     4261 cgcaagatac tttttaatgg aaaagagata caagtgaaca gcggagaatt cgtcacaggg
     4321 cgcgatgcta tcacatctga gatgaacgaa ggcgtgaaac gtgaacatca agtgaacagc
     4381 ggttctgtat ggagatggct aaaacaattt gaaaaaaacg gaatgttgaa catcaaatca
     4441 actacgaaat acagcgttat atcaataaaa aactggtctt tgtaccaaag cagtgaacaa
     4501 caaatgaaca tcaaacgcac aacaagtgaa caacaagtgc acacaaacaa gaatgaaaag
     4561 aattataaga atgaaaagaa taataaaaca tcatcgtcgc aaccacgcaa aaagcgtgtt
     4621 tacgacaccg actcagttta ctacattctc gcggaggagt tattcaaaca gatttgtcag
     4681 aatcaggaaa tcaaaaagcc gaatctgcaa agttgggcag ataacattcg gaaaatgatc
     4741 gagatcgaca agcggaccga aaaccaggta cggggaatga ttgaatggag tcagcacaat
     4801 gtgttttggg catcgaatat tttatctgct aaaaaattgc gagaaaaata cgacacaatg
     4861 gcagcgcaag caaatcgtga ttataaaaca aaacaaacta aaacgcttga atacgagaaa
     4921 tttagcacag atgagttgcc tatttgagag gaggcgtagg catggaaact gttggggaaa
     4981 tcatggaaaa gctgatacag aaagtgcttg tccaacgtgg cgaatgtcct gaatgcggac
     5041 agcctttgta tggatggcgt acgaagaacc ctgatggctc agaacgttgt aagccaacct
     5101 gtatgcaatg tgggtataaa gcattgcgag ttcaggaaga cttacaaaca gaacgaattt
     5161 acaacgagag cctgaaagca agagcaatca attttttcaa aggtggttct gttgtgccta
     5221 atcaagcgtt gtttgattgc acattgcaga attatcaaat tgtcgatcaa gaaacaagac
     5281 aagcggttga agtaaccaaa cgctttgtta attcagtctt gttaggaaat ccaagtcacc
     5341 ttgttttaac tggaaaacaa ggaacaggta aaagtcacct agcgatggca gcggcttggg
     5401 aagtcttgaa gcgatcaaac tacgataaaa aaatcttatt tatcgggtta caggaaatgc
     5461 tggatcaaat caaattttcg tacaacaatc ctgaactcag aaaaacgatt gagggatcgt
     5521 tgattgcaga tatcaaaaca gcggatttag tcatcattga tgatatcggt tcagaactag
     5581 gaaaagatgc atcagatagt cgagcgtttg gcataaacac gctaaattcg ttcttggatg
     5641 cacgacagaa cctagcaacg attatcacaa cgaacttgct tggtgaagaa ctgaaaaaag
     5701 cttacggtac gagaacaata tcaagaatgt ttgtcaactc tgatggattt acgatggtat
     5761 tttctcaaac agcagacaag cgcataaaac cagtcaaagg agcgatcgca tgaatttgac
     5821 taaacaagaa aaaagaatct gtcgtcagat aatgttgcaa attataggtg atcgtgacat
     5881 ggacgaatta tctcaacaag agagtcgaat ggttcggaag ataagtaagc taatcggtgc
     5941 agatacgatg gatcgatcca agccattacc caaactgaat tttgaatcat ttacgtttga
     6001 gaaatatagc catctgagag aggtaggcta tagtgtaaat gctattcgaa aagcacttgg
     6061 ggtaggaagt agtgtatggg caaaatggcg gaaagaaaat gtaaatgagg taaatcaaaa
     6121 tgattaagct aataggcaaa gagacgctca aggtttacta tgtagcggaa acaaaggcag
     6181 aagtcagccg ttggctattg aaaatgttta ataagactag agccaatgct tcagtaagtc
     6241 agaaagcaag tgtatcagaa acaggcatgc ctgaagcaat gtggatttca aggaggaact
     6301 gagatggcaa tgataccgaa gtttagagcg tgggatagag aaaaagacag aatgatttat
     6361 cctagcacga caggagtttg ttttgaaatg aatgatgatg gtataagtgt gcttgatatt
     6421 gatgtagatt atcccaatga ctatggtttt cccaaaatag cttcaatcct catgcaatct
     6481 accactctga aggataagaa tggtgtggag atatttgagg gggatatagc gcttatcact
     6541 cataaatcaa cgaactacgc cgatacttat tggcacagtt acgtagaagt ttttagagca
     6601 aaaaacggag cctacagaat tcgcggtgaa cacatctacg aaacagaatt atatagtaat
     6661 agaaaacaat tgacggtagc ggggaatgtt tataattcac cagaactttt gaagagagat
     6721 cgatttatga ctactaccga aaatccagag ttattgaagg aggaacagcg atgaataaac
     6781 aggaattgat tgataaatat actgcagaaa tatctagatt aagaccttat tgccctaata
     6841 gacatttaat tagcgatcaa cttaaatatg gtctttataa agaaatccta gaagatttaa
     6901 aacaactaga cgaaccgcag aaaccagcgg tgcctaagtt tgtggcggaa tggatagaat
     6961 acgctaaaaa gaaaggcgat agtctagcta tttcattcaa gccgtggaac ctctacggtg
     7021 ttgagtatag caagactgat agatggattg aagataatca aggaacgttt gctcgtgctt
     7081 ggctaattgg ctacgaggtc gagaaagagc cgttgtatta tgtgaagttg ccagttgtgt
     7141 attttaatca ttgggattta gagacgtatc taatgaaaga tgatagagga aatataacaa
     7201 ttgcagacaa caacgatttt gatgatatga aatttacgga atcagaaata aaagcaattg
     7261 atgaaagata ctggccattt gctgtgccag tggaagaggt ggcggaagga tgagcaatct
     7321 taaagttgga gatattgttt tttacaaagg tgacgaaggc atcatccgag aagttatgac
     7381 ggatggaaaa gaaaattttt atagggtaga tgtcagcaga aaatctatac tctacctgta
     7441 tgaagacgaa ttagatttag gtactcaaaa actcaacgac aaccagaaaa tggtgtttga
     7501 ttatcttaaa tcaaacgttg aacaaggtgg caaatcagtt atgtattcga tattccttct
     7561 aggtgattgg gactctcgaa ttggagcagc aagaaacgtt gatatagctt actgtggatt
     7621 aaatggtaag caggaagcag aagtcctatc agcattcgct caatggggat tagaacagga
     7681 ggaagcggaa tgaaatacga aataccacta agtgaagcag acgtccagtc aattattaac
     7741 ggtcgggagg ttaataaaaa acttcctgat ggtactgaat tagtcatcag acaaagttat
     7801 ttgaaagata tggcagctcc agtattaatt gatcgtttta acgtgactga ttctgtggta
     7861 gagaaccatt taaaagaatt tcgatcaagt atagacgaca ctttcagatt agggagttga
     7921 ttgacaatga acaacagaca tcgcagaata acaaaactaa gaaaacagga actgaatgta
     7981 ctaaagacaa agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca
     8041 agtcagtgtg ttgctgatgc gagtaatgct attcgtaagt ttgggatttc gatattaaat
     8101 gatgatcgta aatgggagga aatgagatga aactaaaaga cggattttac gctagtagtc
     8161 acggcatcgg cggtttaatg ctagatatgc cgacaaagaa tcctaaaaca cgtaagaaac
     8221 caaaagtcaa agtcggtgac atggttcgct gcgaagcaga agggttcatc tatccatttc
     8281 gtggatatgt agaacacgtc tataatcact cagcaatcat tcgtattgaa aacacgatgg
     8341 aatgtgataa atggacagct aaaagcaaag agaatttagc agtggtgaga ttggtggata
     8401 ttgaactaat caatgacaaa taaaaaagcc ggatcgctcc gactgattca ataaattcca
     8461 caagtttatt atatcacata aaaggggcgg tttgacttga tgcaattgtt acgagaggta
     8521 gctttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg gcgtttggag
     8581 cggatggcag gtcgctcttt gatagatatt aagtcgccga ttattacgga catgccgaag
     8641 gcaccgaagc acggcaataa ggcagaagac gcgatcattc agatgatgga tatagaagca
     8701 gagagagacg cgattttagc ggctttgatg gcgcttagtc tgattagtcg tcagatactc
     8761 tactacagct tttgtgtgcc agatagcttc tcaaactaca ggattagccg tgaagtgggt
     8821 tattcagaaa gaagcataca acggatgaag tcggaagctc tgatagagtt tgcagaagca
     8881 tataaacatg ggaaaataat tgcttataaa taggaggact atataatgtg gaaagattat
     8941 gtatcattga aagagttgaa aaaagatctt attttcaaaa aaatcgtaga atggtcagag
     9001 agtgaattaa ttttagaaga tggaaccaaa atggaagttg tatgtagcga atcagattgc
     9061 tgcgcatggg ccgaaggtga attcaaaaat gtaaagcttg atgcagtgat tacagatata
     9121 aagatttttg ataaaggtaa ccgtctctat aatggcgatg gacatagttc ttatgccgaa
     9181 gtcgttgttt atcataatcg aaatgagatt gctaaagcgg aatgtacagc aaacgatggg
     9241 aatggaggct actattacag tgtctgtgct ttaaaagtca aagaaaaact ttgtgtagtg
     9301 actgatgcat aaaaaatggc ggttttttgg cggaatgatg gcggttttta gctatttacc
     9361 agtgatatta tggtagtgtc gaaagattag tgataggtct gagacaaaat aaaatgtaag
     9421 ggaggaaatc tccctcatcg tttaattaag cttcgataga cagcagcgga aatattaaga
     9481 ataaggatgt gaattttaac tccttctaaa ttgttcttat tatctatcat ccgttgctgt
     9541 ctattgttat tatgtcactg tggcggaaag ggtagacgct taaaaataag gtcaatacgt
     9601 cgagggatag ccttaacgtt ttatgatttg accatgcaag gttcgattcc ttgccagcga
     9661 cttagacgaa aggtagctta aatattaacc acatcaacat cgtcagcgag ttgataaagg
     9721 taactagcag actctaggat ttaagccagt cttaccgatt agtctgttat agtagcaagc
     9781 ttgctagagg tagctcctct ggttgacgtg cagctcagtt ggtagagcgc ctgactttta
     9841 atcaggaggt cgccggttcg aatccggtca cgttaataaa gaacctacgg aaacaatcaa
     9901 tcttatcgga tgccgattgg ttggctgaat taagcagtgt gtctgtgaat cagttaatag
     9961 gcataaaata ctagcacaga cgtgtgccac tctcaggtgt aggttaggag agaaacatta
    10021 gcaaccgagg gttggaaatg ggcgctcaaa gtacacgagc aaggcgaggt cgatagtaat
    10081 cgatggaatc ggtgtaggtt gctattacat aattgattgg gttagattga gttttgggat
    10141 tcggtacaaa tgaatcgtca aatgactcaa gcacaggatc ggaaacgtcc ctgcctgtgc
    10201 attacatatt agatcactcg ttgagtggtc tttttatttt agaaaagaga ggattttgaa
    10261 aatgaatcat gagaagttta tcgaaaaatg caaggctatt gtacgtgaaa gaattgagaa
    10321 tgagattgct gacctaagtg gagcagtacc tgagtttagt gttttcatag tctggtcatg
    10381 caaaacacta cagaatagca aagcattagt cagcgctagc ttaaaaggag caccgtattt
    10441 tgaaattacg ttgaacgggg acaaaggtga aatctatgta gatacttatc tcaaaaaatc
    10501 aaatgaatgt atcaaagtct agcaggtgct aggctttttc tttacataaa ggaggaatga
    10561 tctcatgaaa caatataccg ctaaagattt cgaggaaatg aagcgattaa agaaggacta
    10621 tgaagaagtt gatatggagc taactgttgg agtcattcaa cgaagactgc gggtcggatt
    10681 agagacagca aaggctattt acaatgatct aaatgctatt gaagagaaga atggctaatg
    10741 aggaactact ggtatatatc gctaactaat gaatatcctc gaaccattga tgattgttca
    10801 gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga aatggggaga
    10861 gaagcagaac catgtgagat tgatgcgtgc aagctcgttt attgcggtca tggtttctat
    10921 gatgaaccaa acattcaaaa taatattaac aagaatttga gggattagaa tgcaagaaat
    10981 ggcttaaaaa tctccaatca ggacaaatca agttagacaa agtttccgat ttaaagatat
    11041 taattgaagc agatctaatg ttgaaagata ttgaaaatta gaaaacaaaa ctcaacctaa
    11101 gaagattgcg aggtggtgtg tattgaatgg caagacaacg tgatccaaga cgtgatgaag
    11161 ccaaaagaat ttggttagaa tccaacggag aaaagcagtt aaaggaaatt gcatctgaat
    11221 taaatgtttc agattctcag gttagaaaat ggaaatcgca agacaaatgg agcgctgaat
    11281 tgaaaagtaa cgttaccaat ggcaaaagta acgttactaa tcaaggtggc gctcctattg
    11341 gtaatcaaaa tgctaaaggt aacaaaggaa atagccgagc ttctccgcca gtgggtaata
    11401 aaaacgcttt gaaaacaggc gagtatgaaa ccatattttt tgagacacta agtgatgaag
    11461 agaaggacat ctattctagt ctgaatgata atccttcttt tgttttgtct gaagaaatac
    11521 gtctacttaa gataagacaa tttcgtatga tgaagagaat ccaacaagct gaagctggac
    11581 taaatgatga agaagtcgaa cgattgcagc agctaagaaa gattaaaaat ccgattgaaa
    11641 aaaatggtaa aaagctagaa atcaagcgtg aggttatgca agatgtgcag attagcagaa
    11701 aaaaacatcg caaaattgat gatattcttt caattgaaga ttcattgact cggattagca
    11761 accagttagc taaagccatc aagcaaatga atgaacttta tatgaatgaa tacagaactg
    11821 atttaattaa agctcagact gataagatcc aagctgagac aaatgaaatt ggcggaaata
    11881 attcaggtga agaaatagaa gaatggaaac aggcagtttt aaatgccgca aacaaacggg
    11941 cggtgaaaga aaatgaataa tgaatttatt ccttttgctg atattggttc tgccattgat
    12001 tattactatg ataaaccagt agctttttgc caagatattt tgcatttgaa tcctgatgaa
    12061 tggcaagaaa atgttttaaa tgatttagct gaattttcaa aggtttctgt tcgttctggt
    12121 caaggagttg gaaaaacagc attagaagca ggagcaatac tttggttctt aacgtgtcga
    12181 ccctacgcta aagtaatagc aacagctccg acaatgaagc aactttacga tgtactttgg
    12241 gcagaggtag ctaaatggtt aaatgatagc ttgatcaaaa acttactgaa gtggacaaag
    12301 accaaaattt atatggttgg tgattcagag cgttggtttg ctacggctag aacagcgact
    12361 aaaccagaaa atatgcaagg ttttcacgag gaccatatgt tgattgtggt agatgaagct
    12421 tctggtgtgt ctgatccaat tatggaagct attcttggta cgctatcagg ttttgataat
    12481 aagctgttga tgtgtggaaa ccccaataat attgaaggtg ttttttacga ttcccacaat
    12541 tcagaccgtg ataaatacag agttcataaa gtatcaagct atgatagtaa acgtacaaac
    12601 aaagacaata tagaaatgat tcttaaaaaa tatggaaaag aaagtgatgt tgctcgtgtc
    12661 cgtatttttg gagaatttcc caaaggtgcg ttggattcat ttatcagtct tgaaacggtt
    12721 gaattggcta cagaaaaaca aattagtgat tctttagtca ataaaacaac ggttgctcat
    12781 attggtgttg acgtagctcg atatggtgat gattctacga ttctctttcc tagaattgct
    12841 accagggcat tggagtatga gaagtattca aaacgtagca ccatggaaac aacaggatat
    12901 gtcatcaaca tggccaagaa tctaatgagt caatatccga gtattgataa agtgatgatt
    12961 aaagtcgatg acactggtgt cggaggtggt gtaaccgacc gcctagaaga acttatagaa
    13021 gacaaacatt atccttttga ggtgtttgga gtgaataacg gttcaacatc agaagacgat
    13081 ttttacgata atttaggtac tcaactatgg ggaaacatca aggaaatgtt agaagaaaat
    13141 atgacagcaa atcttaacgg agaacagcct gttattgaat tgccttctga tagttcgtta
    13201 atcaaagaat taagtactcg caaattcaaa atgacaagta gaagtcgtat acgtttagaa
    13261 agtaaagatg atatgaaaaa gcgaaatatt ggtagtcccg atattgctga cgcactggct
    13321 ttagcgtttt atgagccacc aagtcactat caatttattc aattttagga ggtgagcttt
    13381 tgattagtac agtattatct ttagagagat ataagaagct acgtgttaaa tatgcaacac
    13441 aaattgaaga tggaatgttt gatccgaatg gttttataga agatatgaaa ccattttttg
    13501 ctgatcgcga aagaaaatat ctagcttata ctagcgaaaa gaatgaaata gataatagac
    13561 caaagcctaa cactgatatt gtaaaagtta ataataaact tcatgctggt atgtattcaa
    13621 ttgtcgtaga ccaagcagtc aatcatttta ctggtatacc tatcaaatgg gattacgatg
    13681 tatcggaaca aaaaagaaca ctcattcaaa gattaaaaga taaattctta aaaaatgata
    13741 tagaacttcc gacagttcca gaagctttta acaaattaac aagcaatctt gattccatga
    13801 gattcgcaat gcttgattct gaaactgcaa cttttcaagg agcttgtgga gtagctttcc
    13861 gattgttaga gcctgtggaa gaagatgatg gttggaagtt aagagcaagt aatattgaac
    13921 cttggagagc agaaagatat ggaaatgccg gaatctatat caaagaaaag tatgactctt
    13981 accagaaaaa attttttcaa gaaatgaaag ttatgacaag aaataaaatt ctgacatatg
    14041 cttgctatgg tgatttgaat tttgttacaa gcggaacgtt caaaaaaatt gatgagaccg
    14101 ataacccttt ggggacgatt accttggcag aatttaaaaa taatacgaat cgttattgtg
    14161 attttgaagt agctgaggaa attggagatg caattgatcg tgctttgtct gatcaacaaa
    14221 acgaaatcga acaatttaag cttgcgtaca tgcttgttac tggcacaaca atgagcaaag
    14281 gcactgctaa agaaatgatg aatcagctag ggattatcaa cttaaaagac cctactgcaa
    14341 aagctgaata tgtcacaaaa aatttagcaa aagatttcaa tgaatatcat atggatctat
    14401 tgaaaaaaca gttttacacc atttgtaaag caatcgattt taacgatgag gtgtttaaat
    14461 caaacagttc tggagaagct cgcaagtggc aaatcattag cctagaagcg aaaacaaata
    14521 ctaaagagca atattttaga gaaggattga aagaatgcgc agaaacaatt gctgcttttc
    14581 ttaagtttca tgacaaagta gaaattgagc ctgaaaaaat tatttttact ttctctagat
    14641 ctttgccaac tgatttaagt tatttagctg aagctttacc taaacttgca ccatacgtat
    14701 ccaaacgaac tattcagagc caaattccat ttgtaacaga tgttgattat gaaaatgaaa
    14761 tgatggaatt ggaaagtgga agtgcttatc cagatagtga atacaatttt ggcggaggtg
    14821 gcaatagtga cgatagaaac gaagtattgg accaaacgtc gagaactgga ggatcaagca
    14881 aggctcaaac aagaaaatca gacacttaaa aaattaacta gtgtatttcc tgaagcactg
    14941 aaagagatac aagcaaaact attatcacaa gctgacttac acaatatcac ttatccagaa
    15001 atgatggagt tttatagtac aagtaatcag aaaaaatatc gtgaatatgt ggaaaaaaat
    15061 tataagtcat taaaaatgta tgatgcaaaa tacaaagagt ttatcgatga attttttcca
    15121 ccatttgact atgcaaaagt caatcgctta ttacaaatac gatcagatgt atttaaaatt
    15181 cttgcagaat atgcgatgga tgcagatgtg aatcaatatt tttctgatcg cttagaggaa
    15241 attcttcaaa gaacatattc ttctaatgct aatgtttttg ttcaactttt aaacgttgat
    15301 ataccaaatt atttaccaga aaatgaaatt aaacattatt tgaactatcc atggtcagga
    15361 aagacatttt caagaagact ttggggaaat atttcatcgc ttgaacagaa actctctaat
    15421 gctattgtaa aaagtgttgc tagtggagaa ggcgttatac acgcattaaa caccatgaga
    15481 ttagactcag aaatttgtga catgtttaag ttagaagaat caaagtataa caaagcgata
    15541 gaaaatctcg ttcgaacgga gtatgcaaaa tttgcacaag atggtattga aaaatcatat
    15601 ttagaaacag gtattgagga atacaacgta ttgactgcaa aagatgagag agtttgccgg
    15661 atttgtggag gaaaggcaag taagaatccc tataaactga aagatgctgt cataggtgaa
    15721 aatcgagcac ctttccatag tcgttgtaga tgtacggatg ttcctaattt accaaaatta
    15781 ggaaaggata ttgatgaaga atatgaccgt ttatttggcg atttattaga tgagtttgca
    15841 catgattctt ttggaattaa tttgaaacgg aggaagtaga atgaaagatt tttttgaagc
    15901 agtactaaca attaatgtaa atgctgatat tgcagaagcc tacaaaacag ctattgagtc
    15961 tgagaaccat cctaatggct tgagagacca ttggaatggc aattatgcct acgtggttat
    16021 tggcgatcaa actgttaatt atcaagataa tactccagtt gataagaata ccgttaattt
    16081 aacgattcaa ttattatctc attcattacc aaatttaaaa gaaacagttg attggtatga
    16141 aaagatggga tgtattgttg ttagaactga ctacaaagaa ggaaagtcta gtaattaggc
    16201 ttttttattt tgtccgaaat gacgttaaac tagcgcaata ctgggcttgg ttgaatggtg
    16261 gggcgcaact attaaaactc aaagcaatgc ggggcgtgaa aacgaatcgt ggggcgaaag
    16321 gagaatgagc atgaaacaca aatcattaat gccaatgaat ttgcaatact ttgccgaagg
    16381 tgacgatcaa aagttttctt ttgatgactt caaatctttt gtggaatcca atgaagaagc
    16441 tcaaaaattt attcagtcac aatctcaaag tgttgccgac aaacaattgg aagcttggaa
    16501 acaaaataac ttagataaaa ttaaacagga taccatcaag gaatatgagg aatctaagaa
    16561 aaacaagtca cctgagcaaa ttcaactgga aaaactacaa gctgaatttg aagcagaaaa
    16621 agcgttgcgt gtgacaagtg ataataaagc atttgttgca gaacaaattg ctggattaga
    16681 actagatgga gagttaaaag agtctatttc tcaatttatg ctaaataatc ttgttagttc
    16741 ggatacagat ttcactaaga atgctgttga aggttttaca agtgtcttga atgcaattaa
    16801 tgagaaacat gcagatgcat taaaagaact acaaatgaag tctgcatttg gtggaactca
    16861 acaatcgaat aaccaggttc agcagaacaa tgaaacatta acaaatccag aagaacaatt
    16921 aggacaaatt cttcaacaat ttaactagga gagtgaaaaa ttatgaaaaa aacatcttta
    16981 aataatttag agtatttgga tatttcacca gcgattaatg ctatgcaagt accaaataca
    17041 ccttttttaa gctatttatt tggtgctgga aaaacagagc cagcaaactc gacagaaatt
    17101 aaatggcgtg aatatgatat caacaacgat gattcttctg aaaaacttga gggcggagaa
    17161 tatccagatg ctgaatcagg tcgaacttgg tttaacaact atactgaaat ttttagaaaa
    17221 tcaacctctg tatctggtac attagatgct attaatgtga atggagtcgg aaatgaatta
    17281 actaatcaag tagcactacg tggtatggaa atgaaaattg atttgaaccg aaaattgatt
    17341 actggtgtaa aagctgatga aaatggttct aaaggtcgtc gaatgaatgg gattttgaac
    17401 ttgatcaatt cagcaaataa ggcagaaaca gccactgcgg gtgcagtaac aagaaaagat
    17461 atcgatactt tatttaaatt gatgtatgaa aaaggttata tgggagaaaa actatgcttg
    17521 atttctccag atatgcagga gttaatgact gatgagttag atggaaaatc aacaaaaatt
    17581 gttcagttcg gggaaagagt aacttttgga ttgcaaattg gaaatatcgt gtctaattac
    17641 ggtacaggta ttgctctact agaaccatca ttgccaaaag gaacaattgc cgcaatcgat
    17701 actaattatg tgaaactacg tccattacgt gaatggagag cagaagaact tgcaaaaaca
    17761 acggattcta gacgtatcgg tcttgtaggt gaatactctc ttgaatacaa tgcttcaaac
    17821 tctggggcaa ttttaaattt aaagtctgaa taaaagggag ttagtactcc cttttttgat
    17881 aggaggaatt acagtggtaa aaaaagatga aactaaaaaa gatgaagtcg tgaaatatag
    17941 agtaggtaaa actaaaaatt ttgttgggtt tgttcatcct aaaactcgta gatttatcac
    18001 agcagattca aataacgaat ttatcatttc tatagatgat aaagaagcaa ttgcaatttt
    18061 ggaagatgca attgatgtta atgaaattta ggaagtgatc tgatggatga atcgctaaaa
    18121 acggaaatca ttgagtctac aaaagaagat tttccagatt tgagtgaaga acgcataact
    18181 aatttattag aaataatttt gctagaaatt gaatcataca acacttgtaa aaatgatatt
    18241 tcatgggaaa agttaaaaag cgtgattagt gaagtgttgt ataaaataat aaaaaatgaa
    18301 tcagaaaaaa cagtatcttc agttagacgt ggcgatacaa cgattagtta tgcttcaaca
    18361 acaaatgatg ttagtgagtt acttctaggc tatggtgatt tgatacgaag agttattggt
    18421 tgtggaggat tggagttttt ttaatgaatg aagcagatat tttagaaact acttatgaag
    18481 atagttgtat tattgaaaga ctgacggaca ttgaagattc taatacaaat attactattc
    18541 aagattacaa aaaagtatat gataatccta tttcttgcgc tctttcacaa ggtcaaatcg
    18601 atggactagc agtcatagaa gatggagaga tggtaaatgt ttcaactgac acatataaat
    18661 tatttgttca tcctaagatt aaactaaaaa aaggagatcg aataacaata actcaaaagg
    18721 ccagtggctt aattttttct ctatttgcta ctaagccttt ttactatcct agtcattgtg
    18781 aagtgaattt gataggaagt gagaaaaatg ggtaatctca aatatgaatc aaatgcagaa
    18841 aagatcattg agaattttaa aaatatgact gtaattgctc aaaaagaagg aatatccttc
    18901 gttaacgatt caatgaataa agtcgttagt ttagctaaac cattaactcc tgtaaaatca
    18961 ggtaatttaa ggcgaggata tcgtgtagta aaagctagaa aactatcaag cggtcgaatt
    19021 gttggagcag ttataaacaa tgaacattat tttaaatatg tagaagaagg tcataggact
    19081 aaaaatggtg gctttgtcaa agggaaattc atgttaactc gtgcaacgaa tcttgcaaat
    19141 atgtcttata ttcctcgaag atttaaacaa atggcaataa aaatcgttaa gaaaggaaag
    19201 taacatgaaa gatgaaatca ttgctgcaat cagtagcaaa ttaaaagaaa tctatcctga
    19261 tgggacaata tatcttgatt cagttatgca gtcaactaaa gatttttatt tcgttttatc
    19321 agtaatggaa tctggaactg aaaatgtagg aattgatatt caaaatgttt ctttcttaat
    19381 tgatattgca ttgattgata ataaacctaa tagaaattta attaatgaat tagtctcacg
    19441 ttgtgggact ttttttaata cgattacaat cgatgagcaa atattatttc caaaagccta
    19501 tttacctgat gaagcagatg gtgttcaaca tattcgtttt acattagaat ttccacaata
    19561 tattgaatgg agtgaaagat aaatgggaga aaaaagaagt aaaaccggaa ttatttctgt
    19621 cgaaaaacct acttggtttc ctttggaaga tgagacaggg aattttccgg tatatggtac
    19681 tgcgttgcca atgggaacag cagtaagtat caaaccaaca gctaattatg aaacaacaca
    19741 agattatggg gattcagtgg ttcaagatca atttacagct tttggtggtg cagaagttga
    19801 actggaagca aatggatata ctcataaagt tttatctaca attactggag ggaaaattgt
    19861 taaaggtggt gccttgcgtt ctggagaaga tattgcgcaa gatggtgcgt ttgcatatag
    19921 acgtaaaaaa tcgaatggga aataccgtta tacggtcttt tataaaggac agtttgctct
    19981 agattcagat gaaacatcaa cgattgaagg aagtaaagta agttttactc atccaacttg
    20041 gaagggttca tttgtagatg taccaggact tggatatatg tattcagttg atgaagatga
    20101 tgaaggcgtg gataaagcaa tgatcggaaa ttggtttaca aaagtagcaa ttccaattga
    20161 agaagcagaa ctttcaggag gtacagaata atgtcaaaat atcaaacaac gattaaatta
    20221 acgaaaaaag atgaagaagg taaatatgag caagtacaat ttaaatctgc tgaattttta
    20281 ccaggaacag ttgtagaaga tgctgcagga gttatggaag aaatgcaaac agcgactgat
    20341 aaacaatcag ttaaaaaagc tttaagtcgt gcttattcat ttattgcaga tactttattt
    20401 gagggacaat ttactggtga agattattgc aaaggaattg atgctcgtga gattgcctca
    20461 ttgacaggaa aattattgaa gtctgttact gcaggttttg atgaaactta tacagagacg
    20521 aaaaaaaagt aagtgaggcg ctcaagtcac cttcatttaa gtattcgatt acttaccgag
    20581 aattagatat aaagacgcaa ttactcgaag caggttggac gttaccagaa attgaacgta
    20641 ctgacttgga tgagttaatg cgtctttttg cttttagaga tgcggtaaaa gagcatgaag
    20701 atgttgagta ctatgataat ttcactcaat tttaggaggt gatactttga ataacgatga
    20761 cctaattctg aagatgatac tagatgaatc tggttttact gccggtatga ataatgctgt
    20821 caaaaagttg aactcttttg atgaaacaat cgaaagaaaa agtagaaata gtggcagttc
    20881 attaggtagt atttggaaaa tatttgctgg aagttttcta gctagcggag taactagaat
    20941 cgtaggagct ggctttgatc taatcaaggg ttccataagt ggagcggttg atcgagtaga
    21001 tacgatgaat aacgctctac gaaatttcca aaatatggga tttagtaatt cagaaattat
    21061 gaagaatatc ggaaagaatg ggcttttatc tcaaggtatt caaggacttc ctactgcttt
    21121 gaatgatgcg ataagtcatg ttcaacttct tgcttcttct acaggtgatt taactcgttc
    21181 aactcaaata tttaaagctt taaatgatgg aattcttggt ttcggtggtt caactgatca
    21241 agttaatgaa gctgttattc aattatctca aagtttctca aacggaaaag tagacgcaca
    21301 aacttggaac tcaatgatca acgctcaact tggacctact ctttctgcta ttgctaaaaa
    21361 gatgggaatt acaatgggag atctgaaaga aggtttgtct caaggtaaga tttctgttga
    21421 agaattccaa aatcaattaa tagaaatgga taccaaaggt ggcggaggac ttaaatcatt
    21481 aagtcaaatc gctaaagatt ctactaaggg aattaaaacc tctatacaaa atgcaaaaac
    21541 agctgttaca cgtggtgttg gtgaggtaat agaaggatta aataaagcat tagtagattc
    21601 agatttaggt ggatttaagg gaattattga taaagtgggc agttcaatgg aatcgttctt
    21661 aaaagtaatt gctgcaaata ttcctacagc agtatcattt ttaagtaatt tatttaacga
    21721 aattcaaaaa tttggttctg ctttgaaatt catgatgccc tttcttgttc ctgcagctac
    21781 cgcttttgga gcgttcatgt ttcaacttaa aggtatacca gcaataataa aaagcttcaa
    21841 taattttaag aatgccataa ttggcgttgg aaattcatta aagattatgg gggcaatagc
    21901 tgctgctaat tcatttgttt taattattgg tgcagtcgtt ggtgcaatag ctgttttcgg
    21961 ttactttatg gcaaccaatg aagagtttag aaacaaggtt atatctattt ggaatgatgt
    22021 gaaagattcc gtaattggtg tattaaaaaa tataaaggac tggggaattg atacttggaa
    22081 ttctgctaaa gaaatggcat caaatgcagt tgagggtgtc aaagatgctt ggtcaggaac
    22141 aaaggaatgg ttttctaaca cctggaaagg tattaaggat ggggccacag gtttatttga
    22201 taaaacagta gagacttcaa gaaatgcagt cgatagtgta aaaaatgcat ggtctaatat
    22261 gaagaaatgg ttttctgata cttggcaaag tataaaagat tcagccagag aaaaatggaa
    22321 tgaaattaaa ggttctatta tgtcggttgc tggtccattg attacaggta taaagaatgc
    22381 atttttacat gttacttttt atctagagac cttatggaat aatttagttg agattggtaa
    22441 aaacgtcttt gagattttaa aaaatgttat acttgcgccg gttttgttta ttacctccat
    22501 gatttctggt ggatgggaag aaacaaaaaa taatatgatt gctgtttgga ataatattaa
    22561 agaaagtgcc ataaatattt gggaatctat aaagaatata tttgtaagtt attttacaaa
    22621 tatttatttt gctgcactca atatttggac agggttcaag cttacattga taaatatttg
    22681 gaatgaagtg gtaaatcaag ctaaatcgat ttggttaaac ataaagtatt tttttattaa
    22741 tctttggatt gatataaaat attttgcaat tcagaaatgg attgaattga agttttctat
    22801 tattcaaact tggatcgatt taaaatataa tgccattact acttggaata atattaaaca
    22861 gtttttcaaa gatacttgga aaaatattaa agatacggca tacaatacgt ggatttctat
    22921 aaaaaatacc atgattaata catggaataa cattaaggac tctttctgga atattgttac
    22981 tggaattgtt aattctgctg aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc
    23041 tattaatcgg gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg
    23101 caagaacatc attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga
    23161 tactatcaaa ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca
    23221 ttctccatca agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt
    23281 tggtattgat aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc
    23341 gtctgttcaa cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa
    23401 tatatctgct agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc
    23461 tgattcaatg gttcggaaga ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa
    23521 acgagatgct ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta
    23581 ttaatcaaca ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt
    23641 cagctagtcg agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata
    23701 aaggctatta cagaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg
    23761 atgtggtgca ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg
    23821 attttattct ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg
    23881 aatttaaagg aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagcattc
    23941 gaccatttaa ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa
    24001 tttataaccc agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg
    24061 cttcttttta tattaataag gacaaatatg atttgaaatc gttggacaga gaattattta
    24121 tagattctaa attagaagag gcttaccgaa aattagatgg taatttagaa catcaggatc
    24181 aagtcacttt atttttagat tttccatttt tatatcctgg aaaaaatgaa attaaatgga
    24241 cgaaaaatat tcattcattt gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag
    24301 aatatatagt cctactgaaa cagatttctc aacgaatggc ttaggaattt taaaggattg
    24361 tacaagatgt gaaatatatg aagtagctaa tggaaaatat gaattagagt tggattatcc
    24421 tttaggaact agatttgatg aatattttga aaatgactat caaataaaag caaagccaaa
    24481 cgatcaagaa gagtatcata tcttttttat tgatgataaa gatatagata cttttttaaa
    24541 tacagtaact atttatgctc agagtcgtac aaatcgactt ggaagacggg tagtcactct
    24601 tgcggaagta gactctaaaa ctggtcaaga agcgatatca attattgaaa ccaaaatgga
    24661 taaaaaatct gacatacgac tttattctga tattacggcc gtttctagca caatctttga
    24721 agcgagaaat gttttaaatt gtattgctgg tgaacaagga tcattgcttc aatattgggg
    24781 tggagaaatt aaacgtgaac catttaagct ttctttgtta aagcgaagag gtcgcaataa
    24841 tgttggaacg attcggtatg gaaaagatat gtctggttta aaggtcaaat tagattggac
    24901 aggtataaaa acaagactta ttccatatgc tgatcctcaa agtgaggtag gtacgactaa
    24961 tcgaatttat ggttcgccag tagatagtgc ctatattaat aactatcctg atgtgtatac
    25021 agagcatgtt cagtttacag aagaacaagg agtaaaagac attaagagct taaataaaat
    25081 agctaaaaat tactttaaaa cgatcaatcc tggctgtgat aagcctaaag tttctattac
    25141 ggttgaattt gataagttga ctgatagtga agaagcgaaa gaatttgcga agattagaaa
    25201 ttatggtctg tttgatacat ttaaaatata tcataaaaaa tataatattt atcttgaatc
    25261 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
    25321 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
    25381 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
    25441 tcaaggcagt gcaggaggat atgtaagttt atggccaaaa gaaaaacctt ccaacatctt
    25501 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
    25561 gattgctttt tctaagaaag gatggcgggg gccttttact tcagcttgga cattagatag
    25621 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
    25681 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
    25741 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
    25801 aattggaacg attggaacaa ctgattctgc tggtaatcct tttcctgggg cttctactcc
    25861 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
    25921 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
    25981 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
    26041 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
    26101 agatgcagaa aagagagctt ggagaat
//
LOCUS       DAFFFQ010000034        26027 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000034
VERSION     DAFFFQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.034"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="LQB69_001507"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="LQB69_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL3525677.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="LQB69_001508"
     CDS             808..1242
                     /locus_tag="LQB69_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL3525678.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="LQB69_001509"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="LQB69_001509"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL3525679.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="LQB69_001510"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="LQB69_001510"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL3525680.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="LQB69_001511"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="LQB69_001511"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL3525681.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="LQB69_001512"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="LQB69_001512"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL3525682.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="LQB69_001513"
     CDS             9513..10439
                     /locus_tag="LQB69_001513"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL3525683.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="LQB69_001514"
     CDS             10621..11514
                     /locus_tag="LQB69_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL3525684.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="LQB69_001515"
     CDS             11648..11773
                     /locus_tag="LQB69_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3525685.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="LQB69_001516"
     CDS             complement(11907..12539)
                     /locus_tag="LQB69_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525686.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="LQB69_001517"
     CDS             complement(12687..13229)
                     /locus_tag="LQB69_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL3525687.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="LQB69_001518"
     CDS             13364..13753
                     /locus_tag="LQB69_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525688.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="LQB69_001519"
     CDS             13757..14521
                     /locus_tag="LQB69_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3525689.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPNIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="LQB69_001520"
     CDS             14650..15597
                     /locus_tag="LQB69_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3525690.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="LQB69_001521"
     CDS             15727..16467
                     /locus_tag="LQB69_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525691.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="LQB69_001522"
     CDS             complement(16758..17426)
                     /locus_tag="LQB69_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3525692.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="LQB69_001523"
     CDS             17560..17847
                     /locus_tag="LQB69_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525693.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="LQB69_001524"
     CDS             18263..19801
                     /locus_tag="LQB69_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL3525694.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="LQB69_001525"
     CDS             19798..20763
                     /locus_tag="LQB69_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525695.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="LQB69_001526"
     CDS             20750..22072
                     /locus_tag="LQB69_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3525696.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="LQB69_001527"
     CDS             22313..23056
                     /locus_tag="LQB69_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525697.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="LQB69_001528"
     CDS             23071..24063
                     /locus_tag="LQB69_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3525698.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="LQB69_001529"
     CDS             24075..24812
                     /locus_tag="LQB69_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525699.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="LQB69_001530"
     CDS             24802..25524
                     /locus_tag="LQB69_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL3525700.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="LQB69_001531"
     CDS             complement(25608..>26027)
                     /locus_tag="LQB69_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL3525701.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8954 a         4425 c         5429 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctaatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata gaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAFFFQ010000035        25806 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000035
VERSION     DAFFFQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.035"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            6..848
                     /locus_tag="LQB69_001532"
     CDS             6..848
                     /locus_tag="LQB69_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3525702.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1795
                     /locus_tag="LQB69_001533"
     CDS             848..1795
                     /locus_tag="LQB69_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL3525703.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1810..2043
                     /locus_tag="LQB69_001534"
     CDS             1810..2043
                     /locus_tag="LQB69_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL3525704.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2374..2958
                     /locus_tag="LQB69_001535"
     CDS             2374..2958
                     /locus_tag="LQB69_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3525705.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3089..3756
                     /locus_tag="LQB69_001536"
                     /pseudo
     CDS             3089..3756
                     /locus_tag="LQB69_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            3767..5182
                     /locus_tag="LQB69_001537"
     CDS             3767..5182
                     /locus_tag="LQB69_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3525706.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            5200..5769
                     /locus_tag="LQB69_001538"
     CDS             5200..5769
                     /locus_tag="LQB69_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL3525707.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5879..7633
                     /locus_tag="LQB69_001539"
     CDS             5879..7633
                     /locus_tag="LQB69_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525708.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7593..9359
                     /locus_tag="LQB69_001540"
     CDS             7593..9359
                     /locus_tag="LQB69_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525709.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            9882..10151
                     /gene="rpsN"
                     /locus_tag="LQB69_001541"
     CDS             9882..10151
                     /gene="rpsN"
                     /locus_tag="LQB69_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL3525710.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10166..10345
                     /gene="rpmF"
                     /locus_tag="LQB69_001542"
     CDS             10166..10345
                     /gene="rpmF"
                     /locus_tag="LQB69_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL3525711.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10377..10754
                     /locus_tag="LQB69_001543"
     CDS             10377..10754
                     /locus_tag="LQB69_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525712.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10775..10924
                     /gene="rpmG"
                     /locus_tag="LQB69_001544"
     CDS             10775..10924
                     /gene="rpmG"
                     /locus_tag="LQB69_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3525713.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10948..11931
                     /locus_tag="LQB69_001545"
     CDS             10948..11931
                     /locus_tag="LQB69_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL3525714.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11961..12083
                     /locus_tag="LQB69_001546"
     CDS             11961..12083
                     /locus_tag="LQB69_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL3525715.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12101..13624
                     /locus_tag="LQB69_001547"
     CDS             12101..13624
                     /locus_tag="LQB69_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL3525716.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13648..13887
                     /locus_tag="LQB69_001548"
     CDS             13648..13887
                     /locus_tag="LQB69_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525717.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14080..14541)
                     /locus_tag="LQB69_001549"
     CDS             complement(14080..14541)
                     /locus_tag="LQB69_001549"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3525718.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14736..14954)
                     /locus_tag="LQB69_001550"
     CDS             complement(14736..14954)
                     /locus_tag="LQB69_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525719.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15072..16688
                     /locus_tag="LQB69_001551"
     CDS             15072..16688
                     /locus_tag="LQB69_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL3525720.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16929..18269)
                     /locus_tag="LQB69_001552"
                     /pseudo
     CDS             complement(16929..18269)
                     /locus_tag="LQB69_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18545..18700)
                     /locus_tag="LQB69_001553"
     CDS             complement(18545..18700)
                     /locus_tag="LQB69_001553"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL3525721.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18996..19328)
                     /locus_tag="LQB69_001554"
     CDS             complement(18996..19328)
                     /locus_tag="LQB69_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3525722.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19842..20033)
                     /locus_tag="LQB69_001555"
     CDS             complement(19842..20033)
                     /locus_tag="LQB69_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525723.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20127..20513)
                     /locus_tag="LQB69_001556"
     CDS             complement(20127..20513)
                     /locus_tag="LQB69_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3525724.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21207..22412
                     /locus_tag="LQB69_001557"
     CDS             21207..22412
                     /locus_tag="LQB69_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL3525725.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22429..22863
                     /locus_tag="LQB69_001558"
     CDS             22429..22863
                     /locus_tag="LQB69_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525726.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23480..>25806
                     /gene="esp"
                     /locus_tag="LQB69_001559"
     CDS             23480..>25806
                     /gene="esp"
                     /locus_tag="LQB69_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL3525727.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8894 a         3698 c         4830 g         8384 t
ORIGIN      
        1 ataatatgct tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc
       61 taattacatc tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa
      121 gaggaatgtc tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt
      181 cttatatttt aggtattaat ttttttattg gtgcgattgt atttggtcta ttagcttcta
      241 tcattattac ttttataaaa agcaatagtg taataaaagg agatacagca attggtatta
      301 ctttcagttc ttttttagca ttaggagtta tccttatagg agttgcaaat agctcaacgg
      361 atttatttca tatcttattt ggaaatattt tagctgtaca agatattgat aagtggatta
      421 caattggcgt ttctgtttta gtgttggtta cagttgttct attttttaaa gaattattaa
      481 tcacttcttt tgatccatta atggcaaaag caatggggat gaatgtcagt ttttatcatt
      541 atctattaat gattctatta actttagtag cagtaacagc tatgcaaagt gtgggaacta
      601 tattaattgt tgcaatgtta attacaccag ctgctacagc atatttatat gcaaacagct
      661 taaaaactat gatatttatt tcggcgacag tcggagcgtg ctcatcattg ctaggtttgt
      721 tcattggtta tagttttaac atagcagcag gatcaagtat tgttttaaca gcggcattct
      781 tatttgtaat tagttttcta atctcaccaa aacaaaaatt tttaagaaag aaggagagaa
      841 agttatgatg agaaaatgga aagcagtact gggaagtctg gggattttga ttgctctttt
      901 tatattcggg gcatgttcaa caaatagtaa agacaaagat acagtggctt cgaacgaaaa
      961 attaaaggta gtagttacta attcgatttt agcagatatt actgaaaata tagcaaaaga
     1021 taaaattgat ttacacagta tcgtacctat tgggaaagat ccccacgaat atgaaccttt
     1081 gcctgaagat gttcaaaaaa cttcaaaagc agatttgatt ttttataacg gtgttaactt
     1141 ggaaactgga ggaaatgctt ggtttacaaa attagtaaaa aatgcgaaca aagaggaaaa
     1201 caaagactat tttgcagcaa gtgatggcat agatgttatt tacttagagg gtcagagtga
     1261 gaaagggaag gaagatcccc atgcttggtt aaatttagaa aacggtatta tttacgctaa
     1321 aaatattgaa aaacagttag cggaaaaaga tcctgataat aaaaaattct ataaagaaaa
     1381 tctagataag tatattgaaa aattggattc tctagacaaa gaagctaaat ctaaatttgc
     1441 ttcaattccg aatgataaaa aaatgattgt tacaagtgaa ggatgcttta aatatttctc
     1501 gaaagcgtat aatgtgcctt ctgcttacat ttgggaaatc aacactgaag aagaaggaac
     1561 accagatcaa ataaaacact tagttgaaaa attacgcaca acaaaagttc cctccttatt
     1621 cgtagaaagc agtgtggacg atagaccaat gaaaacagta tcaaaagata ccaatattcc
     1681 tatctattca acgattttta ctgattcaat tgcagaaaaa ggacaagatg gtgatagtta
     1741 ctatgcgatg atgaaatgga acctggataa aattgctgaa ggcctttcga aataatttgg
     1801 agggtaggta tggtacaaat taaaacgact actatactaa tttatttact agcaattcag
     1861 gaattatcaa aaagaaaaaa gaaattaaaa aataatgatt tagcaaaagt tctaagtgtt
     1921 agtccagcat ctgtaagtga gatgctggct aaacttagat atgaggacta tttagatata
     1981 ggatttcaat taactgataa agggaaaaaa tttattgacg attacaaaaa aggttttaag
     2041 taaaagactt tatttttagg gaggataaga aaatggctat tggagtgatt atgataggac
     2101 ttaccctaat agctggatat aattttttta aatctttctc ggattatttt agaaaaggtg
     2161 aatgatttag atgagtctat taattcttgt aggattgctt tttgtatcag ttattttgct
     2221 ctttctcatt agtttagctc taaggaatga gttcagtaat agacgttaac gattgaaact
     2281 tcaagtcctg tgtaagataa tatagttaat tcatcttatt aggaggaagt acccatagaa
     2341 accagagatt atattacaac tcaccaaaaa ttaatagaaa gtggactaag ccattttgaa
     2401 aaagaaggat ttcagcaaac taagataagg caactgtgct tgaccgcagg cgtaactacg
     2461 ggtgcttttt ataaacattt tacttctaag gaagcgttat ttgaagaaat tattcagccg
     2521 catattaatg cgcttattaa tatgtataat gaagagttta ccaaatacct cttaaactta
     2581 gaaaatatag atatagagaa aagttggaga gagaacttca atgaattaga tccatttatt
     2641 aattatattt ttgagaataa acgtatattc gatttggttt tattccaatc agctggaacc
     2701 aaatatcaag atattgttga aaaaattaca tcatttgtga caaaaaagac agtctgttgt
     2761 ctagataagt taaagactat cggaatgatc aatcaaaatt tagtctttaa tgaagaagaa
     2821 atccacttct acatttactc attctacgct acattctatg atatattgaa gcatagctat
     2881 tcaaaagaaa agactatgct attaaccaga agattgtatc aattttctgt tcctgggtgg
     2941 attgaattac tgagttaatt aaaaaaaagc ttgcattgag aattgttctc agttatgctt
     3001 tttgataaga tcgcactgtt atctgaataa cggtgcattt ttgtcagctt aagataacag
     3061 tgcgatctta ttttttggag gtgaaagagt gtcaaaaatc aatccgttaa ccttgattac
     3121 tttaaatatt tttttccggt cattattttt ttaggaaagg gaaatatatt tcaagtgggg
     3181 tgtttcatta ttgctacagt tttagtattg atgtttcgta aaataaagac atgtatttat
     3241 ttcatcttga tttattcagt tctgttttta gtaatgaaaa ttattgatgc tatcaagata
     3301 gagtttttta ctatattttt tgggacatcc ttatatattg tatttcgaat gttgccggtc
     3361 gtaatgatct catggttgtt ggttagtgtc tatactagca atgaattatt atcctcttta
     3421 gaaaaaatac atttaccgaa aaaaataatg ttaagtatta cagtagtgat cagattcttt
     3481 ccgacttatc gttctgaaat aagaatgatt aaagaatctt taaaaatgag aaacattcgc
     3541 ttgtcttttt ttcagcctct gcaatatttg gaatttctgt tagttcccgt tcttatacgt
     3601 gcaactatga ttgctgaaga aatgaccgct aatgcaataa caaaggggat agaatctcct
     3661 gtgaaaagaa gttcttacta taaaacaaac atgagcttat tagactatat catcctgttt
     3721 tccgttacta tttttttctt aagtattttg atttgaggtg aaagaattgt ttgaatttaa
     3781 caatgtttct ttttcatata aggatgttaa ggaacaagcg cttgttgacc ttaatttttc
     3841 aataaaaaaa ggcagtctta cattgattac tggttcttct gggagtggga aatccacact
     3901 attaaagtta atgaatggac ttattccaag actttatgac ggaaaattgg ttggggaaat
     3961 tttatttgaa aataactcgc tacaatcctt aacacaagaa agtatatcaa aaaatattgg
     4021 ctacgtaagt caaaatccta gaggacaatt ctttacaaca aatacaacga gtgagttagt
     4081 attttcgatg gaaaactttg gatttacaaa agagcatatg gataaaagaa tcaatgaatt
     4141 agctgaatta ttagatcttt caaatatatt agataaaaac attttttcgc tatcaagtgg
     4201 tgaacggcag aaaatttcta ttggatgtag tttatcgcta aactctaaat taattatatt
     4261 ggatgaaccg tcttcaaatt tggacttcaa agcgactaag aaacttgctg acttactttg
     4321 tgatttaaaa aataaaggat ttactattgt tatagctgaa catcgttttt actatttgaa
     4381 agaattaatt gatcaagttc tatttgttaa aaatagaact gtaaaaacta caactattca
     4441 gttgttaaaa actgaaaaca ataacgcatt aaggaaattt gacattttta atagttcgat
     4501 tgaagttccc tatatcgaaa aggtaagtgc agatgtatta aaagttgaag aaattactta
     4561 tgaaaatatt ctaaaagaca taaattttga tgtcaaagtt ggagatgtta ttggtttggt
     4621 agggaaaaac ggtgtgggga aaacaacctt actaagaata ctcatgaaga ttattcgccc
     4681 taccaaagga aagataatcg aaaataaaaa aaatttgtca tcgccgtttt tagtaatgca
     4741 agaaatggat taccaatttt tcacagaatc agtgaggagc gagttagccc tggggaatga
     4801 aatagttagt aaagatcagc aagaaaaaat tttaaaaaaa atggaacttt ataaaatgaa
     4861 agatcaatta ccgtttgatt tatccggtgg agaaaaacaa agattattgg tttctatagc
     4921 ttcattgtca aaaactaatt tatttctatt tgatgaacct acaagtgggt tggattatat
     4981 caatatggaa aggattgctg accttattaa agacttaaaa gaaaagggtg cagtagttat
     5041 agccactcat gatccggaat ttctttataa aacatgcaat agaattattt atttaaaaaa
     5101 tggtggaata aaaaaagata taaaacttag agtagaagat tcaactttga tagaacagat
     5161 ttttaatgac ataacgaaat agaattgagg agaatatata tgaaaacaaa agattatatt
     5221 tttattggtt tagctacagt tatttcccta gtaatttact ttttttcaat aatggttagt
     5281 agtattggtg gggcgtttgg tcacagcata tccccaggga tttttggatt attatctgga
     5341 atcatatttg tttatattag ctataactac cctagaaaag gaatttttac tatttacaca
     5401 attgttttat tgttattttt tacgttaatg ggcggggcgt acttaccttg gtttatcagt
     5461 tcgattagta tggcgatctt agctgatatt attctatctg tatttggata tgatcgagca
     5521 attcctcaag ttgtgtcttg ggttctaatg caactaggca gtgctgccgg acagtggatt
     5581 cccatatggt tctttacaga tcgttttcgt caagactgga tagatagagg acaatctgct
     5641 gcaacaatgg atgcaatgat ccactacgcg gtgggtatat ggggaattat ttcagtgtta
     5701 gttgtagcaa gtttatctat gattggtgta ttgattggaa gaaaagtctt aaaaaaatac
     5761 aaaaaataga acgtagttta ccttctatct gtttgtacat ctctaattta cgctattacg
     5821 gaaagcatta acagttgtaa acgaataaga gaagaaattg aatgcggggg aggaaaaaat
     5881 gaaagtatac aaaaaattat ttagttatgt ccctaaaatg aaatactacg gatggttagc
     5941 cactttattt tctgattttt ctgtcgtatt aacagtctat ggatattaca gtatttacaa
     6001 atttcttcag tctctaatta ttgtaggaga tgaatattta gctaagagtt atgcggttca
     6061 aactgttgct tggttaactt taggagcttt attttatatt ttttctggct tattttctca
     6121 tatccttggg tttcgcttag agactaattt gagaaaaaag ggaattagcg gtttaacaga
     6181 agcaagtttt cgtttttttg atttaaattc ttcgggatat attcgaaaga caattgatga
     6241 caacgcggct aaaacacata tggcaattgc tcacttgatt ccagatagtg ctcaagccat
     6301 agtaacacct gttttatcag ttgccttagg ctttttcatt aactttaaaa ttggattggt
     6361 tctaatcgcc atgttagtaa ttggtgtatt cttgttaaag aaaatgacag agaatacaaa
     6421 ttttattcaa aaatatcaag aatcgttaga tgtgttaagc tctgaaacgg tagaatatat
     6481 ccgcggaatt caagttataa agatatttgg tgctaaagta acatcattta aagccttgaa
     6541 taaagcaatt aacgattatg ctaagtttgc atataagtat tctttgagtg ggaaaacacc
     6601 ttttgttctt tttcaatggc tattttttgg catagtttgc attttgatag ttcctataac
     6661 cttttttata acttctctta ccactcccaa gatcttagct gttgacttgt tgatgttgtt
     6721 ttttttaagt ggaattattt ttgtttcttt catgagaata atgtatgtaa gtatgcactt
     6781 gtttgaagca agatatgcag tcgaaacact agactccctt tatttagaaa tgaagcaaga
     6841 ccaactaaaa tatgggaacg agacacagat taaaaattgt gacattgcat ttgaaaatgt
     6901 cagcttttca tacaaagaag agtgtgtttt agaaaatttc aacttagttt tagaacaaaa
     6961 aagaatttat gcattagtag gaaaatcagg aagcggaaag agtacgatag ctaaactaat
     7021 ttcaggtttc tacaatgtgg ataagggaca gattaaaatt ggtggtaagc ctttaaatca
     7081 ctattcaaaa gacacaatta ttaacacaat tgcttttgtc tttcaagata cgaaactatt
     7141 caatcagagt atttacgata acgtttctat agcaaatcct caagcgccag caagtaaagt
     7201 atttgacgcg ctaagattag ccggttgcga atcgatttta gaaaaatttc ccgaaagaga
     7261 gcatacctta attggtagta aaggagttta tctgtcagga ggagaaaaac aacgtatagc
     7321 tattgccaga gcaattttaa aagatgcaaa gattgttatt atggatgaag ccagtgcatc
     7381 tattgatcca gataatgagt atgaactaca aaaggctttt caatatttga tgaaagaaaa
     7441 aacagttatt atgatagctc atcgattatc cagcattaaa tctgtagatg aaattcttgt
     7501 cttgtcagaa ggaaaaatag tagaacgtgg aaatcataat gaattaatgg ctaaacatcg
     7561 agtgtataaa caattggtca ctaattatga gaatgcaaat gattggaggc ttgacaatga
     7621 aaaattttta taaaaaaaaa tttgctttaa ctgatcaggg cgcagaagct ttaactaaag
     7681 cttctatttc tagttttttt gtatactgca ttaatatggt accagcattc attataatga
     7741 tgctgataga cgaattagtt ttagaaaatg caaagccccg ttggctttat tttgcagtat
     7801 cgtttgttac tttgcttttc atgtattggt tgttagatag ggagtatgaa aacttatata
     7861 atagtactta taaagaaagt gcgcatttaa gagtgcaaat tgcagacgat ttgtcaaatt
     7921 taccattatc ctatttttca aaacataatt tatcagattt atctcaaact atcatgtctg
     7981 acgttgaagg tattgagcat gcgatgagtc atgcaatacc taaagccggc ggtatggctc
     8041 tgtttttccc ttttatttca gtgatgcttt tggttggtaa tgtcaaaatg ggattagctg
     8101 ttattttgcc aacgttattt agttttgtct taatcttgtt atcaaagaaa tcccaaacga
     8161 aagccaatac taaatattac gatactttga gagaaaactc ggaagaattt caagaaacta
     8221 ttgaattgca gcaagagatt aatagcttta atctatctaa aaaagttcaa gacagacttt
     8281 tcaaaaaaat ggaagagagt gaaaggattc atttaaaggt agaattaagt actttttcag
     8341 tcatggcctt atcctctatt ttctcatatg ttagtttagc agtagtcatt ctagtaggtg
     8401 ttcacttact gttaacgggt gaagtgacta tactctacgt cgttggttac ttactagccg
     8461 caataaaaat aaaggattcc ttcgattcta tgaaagaagc agtgcttgaa atattttatt
     8521 tagctcctaa aatacagcga attagagcta tgaaggaaac cagtatccaa gagggatcag
     8581 atagtcctct gaaatctttt gacattgagt tgcgagatgt ctctttctca tatgataaca
     8641 ataccccaat tttagatcat atctctttta cggctaaaca aggagaagtg actgcgttag
     8701 taggtgctag tggttccggg aaaactagta tcttgaaatt agtgtctaga ctgtatgatt
     8761 atgatgaagg ctgtatttta attgatgggt atgatattaa acgtgtatct ccagcttctt
     8821 tgtttagcaa aatcgctatt gtttttcaag aggtaacttt gtttaataca agtattttgg
     8881 aaaatatacg tattggaaat agtcaagcgt cagatgagga agtgaaaaaa gcagctcgtt
     8941 tagctaactg cgaagacttt attgaaaaac tacctgacgg ttatcataca ttggtcggag
     9001 aaaacggaag ttcactttct ggaggagagc gacaacgcct atccatagca cgggcttttt
     9061 tgaaaaatgc gccaatttta attttagatg aaattactgc cagtttagat gcggaaaatg
     9121 aaaaacgtat tcaggaaagt ttaaatcgtt taatacaaga taagacagta ctaattatat
     9181 cgcatcgctt gaagtctatt gaaaaggtaa ataaaattgt agtgatggat caaggaaaac
     9241 tagttgatca aggtactcat aatgagttgt atcgaaggtc agaaatatac aaaaatctca
     9301 taaagaaaac taaactatct gagaaattcg tctatgaaaa agaagctcaa tcacgatgat
     9361 gaaataggct ttaaattaac ctacaatttt tgggatatag ttataattct ttttcaaaaa
     9421 gaataatttt attaaacagc atctaatttt gaatacacga aagaatcagt atgattgaat
     9481 tagttcactt aatgtctatt cttataatat aaggaataat caaagtttta aaaaattggc
     9541 gataaaccag ttaaacataa aagattgaat aaaaagttat agtttctttc atattgccgt
     9601 tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt tgattggact aataaaagtg
     9661 aattcttatt tttcagagcc taaactgcat attcttttaa atgatttgat tatctctatg
     9721 taccgataaa ttcgttttca aatagttatc tattcatatg agttttattt catgttattt
     9781 attgttgaca agagcaactc ttgttggtaa agtatcagtg tcaaaacgta acgattacga
     9841 tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa
     9901 ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta tgcagataaa cgtcaggaat
     9961 taaaagccgc aggagatcgc accgctctcg ctaaattgcc aagagattca aatccgaatc
    10021 gtctgaggct tcgtgatcag acggatggtc gtccaagagg gtatatgcga aagttcggta
    10081 tgtcccgtat taagtttaga gaacttgccc atcaagggtt gattcctgga gtaaaaaaag
    10141 caagttggta aaaggaggaa atatcatggc agtaccagca agaaaaacat ctaaggcgaa
    10201 gaaacggtta agaagaactc atcaaactgt agtaaaacca gaaatttcat ttgacgaagc
    10261 gaatggtgat tatagacgta gccatcatgt atctttaaaa gggtactata atggaaagaa
    10321 agtaattaaa ggagcttcaa aatagtttaa tatataactt ttaggaggga atcaaaatgg
    10381 aaattattta cccaccttta gttgaagaag gattaaagta ttatcttgaa acgacccagc
    10441 aatcgctaga taaatgcact ttttatcgtt cgatggtaga gagaggaatt attactgaaa
    10501 cgggtttacc gacccaacag gcaattgaaa atggattagt caaagattac tatgaggacc
    10561 aaggcttatc atttgatgat tttttaagaa tctatccaat ttttgaagag tatgacgagg
    10621 agttatttca atgtatcgat ggttattggg agatacctgt tgatatgaaa gaaaatttag
    10681 tttcacaatt ggaatctggc gagttaactt ttgaagatgc acaacaaatt caagcatatc
    10741 ttgaagatcg ataagtattg aaggaggaag atttatgcgt caaaatatta ttttggaatg
    10801 tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat aaacggaata accctgaacg
    10861 gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg gcaattttta aagaagtgaa
    10921 atagctaagt ggtaaaaagg tgacaaaatg ggcataccag ttacaatcgt ttctgggttt
    10981 ttaggggctg gaaaaacaac gctaatcaat caagccttgc aagtctcacc ctaccccagg
    11041 gaagaaatta ttataattga aaatgagttt ggtgaagttg ggattgatca caaattatta
    11101 gttcatagcg aggaacgggt gttacaacta aataatggat gtatgtgttg cagtcttcgc
    11161 agtgatttat tagcaatatt gatttcttta ctggaaacaa tagaagaaca tcaacaacca
    11221 atttcacaaa tcatcattga aactacaggt atagcagatc cccaaccaat cattcaaaca
    11281 cttttaacag cgcctcaact aagaggaaga gtatatatcg atagtttatt gactgttatt
    11341 gatagtgaca gttttgaact tattgaaaga gaaccacaag cccttaagca acttgtcatg
    11401 gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt tatctgcact aaaaaaatta
    11461 cagagagaaa tcaaaagtat taacccttta tccgatattc gaatgttcac acatagtgaa
    11521 acgattcaaa aaaaggattt ttataatttg aaaaaattta atcatccctt agtttttgaa
    11581 agtgattcat cagaaaatca ctataatggc aaatactatc atcaacacca tcaagtacat
    11641 gagtttaaat caattctctt aacttcagat caagacataa aaaaagattt tttactccaa
    11701 tggattgact ggctgatttt aaccaaccaa gggagcctgt atcgttttaa aggattgatt
    11761 gctttacaag agcaggattt tatagttgcc atgcaagagg taaacgaaca ggttaatttt
    11821 caatacacca ctcgacaaaa agaccaaacc actattattc taatcggaaa aatgttggat
    11881 gaagaaagga tcaaacagag ttttgataaa ttactggcgg aaagcaaata atgataagta
    11941 gtgattgggg gtaatttcta atggagaaaa cggatatatc tagtgcatat cgacgactta
    12001 aaagcccgaa tattaaaact agaaaaaggg cattaaaaat tatcaaagaa cataaacgaa
    12061 ataaaatgaa gaaattagca taattaagga aggaaaaaga atgaagaaaa tcagtgtagg
    12121 acttattggg atagcagctc taggattatt gggagcttgc tccagcacta atgatgcaaa
    12181 agtatcgaat gataaagatg gaaaattaga aattgtaaca acattctatc caatgtatga
    12241 tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat ttaatggttc cagcgggatc
    12301 agaacctcat gactatgagc cctcagctaa ggatatggca aaagctcatg atgctgatgt
    12361 ttttgtatat cataacgaaa acatggaatc atgggtacca aaagctaaag aaagttggaa
    12421 gaaagctggg cctaatgtag tagaaggaac aaaagacatg attttattgc caggtagtga
    12481 agaagaagat catgatcacg gagaagaaga tcaccatcat gaactagatc ctcatacgtg
    12541 ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc aaggatcagt tagttaaatt
    12601 gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa aagtatttga ctaaactgaa
    12661 acggttggat gctgactata ccactagtct taaagaagcg aaacaaaaaa gttttgtgac
    12721 gcaacacgca gcctttggat atttagcttt ggattatgga ttgaaacaag ttcctattgc
    12781 tggactaagt cctgaagaag agccatcttc tggtcgatta gctgaattaa aagagtatgt
    12841 taaaaaaaat aagatcaatt atatttactt tgaaaagaat gctaatgata aaattgcaaa
    12901 aactttggcc aacgaagctg ggataaaatt ggaagtactg aatcctcttg aaagtcttac
    12961 aaaagaacaa atggataatg gtgaagatta tgtttcagta atggaagaca atctaaaagc
    13021 gttagaaaaa acaactatgg ttgctggtga agaggtagtt ccagaaaaag aagcgaagga
    13081 tgaaaagaca gttgctaatg ggtactttaa agatgccgat gtgaaggatc gcgaattgag
    13141 tgattataca ggcgaatggc aatctgttta tccgttgtta aaagatggaa ttttggatga
    13201 agttttcgat tacaaagcaa aaatcaataa agacatgaca gctgccgagt acaaagatta
    13261 ttatactact ggctataaga cagatattga tactatcaac attaaagaca acaccattga
    13321 ttttgtagtc aacggtgaac atcatcaata cacatacaaa tatgttggat acaaaatttt
    13381 gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt gaaactgatg atgcaggtgc
    13441 tggtcgtttt aaatatattc aatttagtga tcatggaatt gcaccaagta aggcagagca
    13501 tttccatatt ttctttggtg gagaaagtca agaaaaactc tataatgaat tgcataattg
    13561 gcctactttc tatccagctt cattgtctaa acatgaaatt gctcaagaaa tgatggctca
    13621 ttaaaattca atttttggag gaaatacatg gtaagaaaag tattattgtc tatggattcc
    13681 aagaatattg atgcttatcg gcgttatcga tttgcaaagc aaatgagatt aaatgggagt
    13741 acattagctg atattgctgc aactttacat gttagtattg acacagttca tagagtatta
    13801 aaacgggatc ctgattgttg ttttatcaag gtcaatactg gaagaagtag acgtttagca
    13861 aaacggcata atagcttggc caattaattg ataataccag agtagatcat ttttccctta
    13921 tttggatttg atctgctttt tattatgtct tgaattgtca aatcaagcgc aacactttat
    13981 caaagaagac ttcataaggt gttctccaat ctaaacactt tctaggtctg ttattcagtt
    14041 tttcaatcat ctgatttatc gttgagttat ctaccgtagc tatctctttc gattttggca
    14101 agtattctcg aatgagtcta ttcgtatttt aatttgttca ttgttgccaa gggcatgagg
    14161 atctggaaaa taaaagggca tattattcaa tgcttctgta actgcgaaat gattggagaa
    14221 ttcttttcct ctatctggag tgatcgtttt gaccgcctca tttggaatac tgcttaaaag
    14281 ttcaatcatt ttctcgctca cataagcggc tttctttctg gggaccttct ttcctgctaa
    14341 aaggaagcat gattttctgt ctgtcaaagt cactaaacaa gtaccaccag ttttgccagc
    14401 tacaatatcg gctttccaat gaccaaaatc acttctatca tctgcggatt ttggacggcc
    14461 atggatcgaa tgagtaaggc aaatctttcc acgagactcg acatgatttt ttgtatgtct
    14521 tgtttttcct cgatggtgca atgaacggat caggccttta ttgccgtgtg agaggggttc
    14581 tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg ataaaactaa ttgaaaagtc
    14641 catatgttca tgcttcaaac gattcgagat ttcctctggc gaccactgaa aatcgacaaa
    14701 taaatgacga acaattgacc aaactcacgg atcatttaga atgtgataac gtccgcattt
    14761 ttttcttcga taagcataat ttttttccgc tttggaaggt gaatagtttt tttcttgttt
    14821 cttctcagtt catgtaaaat agtaaacggg ctatgctgaa tgtcgttgcc aattatacta
    14881 atagaaaatc cttcttcgtg aagcagaaag attctttctc gttcgagtat agtaagatga
    14941 cgatactggc tcataattat tttccttcta ggagtgttct cgacaaactt atactagttg
    15001 gatatcgatt atgactcatt tttatgtgtt gcacttaaag tgtaaattca aggtatagaa
    15061 agaggttccc tatgaaaaaa atacctatta caatcttaac tggctatctt ggttcaggaa
    15121 aaacgatact tctaaatcat attctgtacg gtgatcacgg aaaggaaatt gctgtcattg
    15181 taaatgacat aggggaagta aatgttgatg gggtattagt cgacaagaaa ggttttaaaa
    15241 gaactggaga aaaacttgtt tcgatgccga atggatgcat ttgttgtaca ttacgcgaag
    15301 atttgattgt tgaactagat caattagtgc aattttctga cttgaatcaa attgttattg
    15361 aagcttcagg aatcagcaaa ccaattccaa ttattcaagc aattatatta gctaaaaacc
    15421 ctttagacat tgatttgacc caacgattag agattggtgc cattattata gtagtggatg
    15481 taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat tcaggaaaga aaagctgaag
    15541 gtaatgtagt tgaagaagaa caggatattc gtggcttgtt aattgatcaa ttcgagtgtt
    15601 gtaacatatt acttttaaat aatagtgact tagtgtcaac caagacgatt tacaaaatta
    15661 acggtctaat tgctaagtta cagtccaatg ccaaaataat taggacaatt aaaggtgaag
    15721 tagacttatc gaaaattcta aatacaaatc tttttaatat agatgagact tcctattcag
    15781 tgggctggtt caaagagtta gagcttggct atcaaaatca tattcctgaa acagaagaat
    15841 atggtatctc aagttttatc tatcgaaatc ggttactcct tgacgcacaa aaatttgccc
    15901 atcttacgga taaaaatttt cctggcaata ttacaagagc aaaagggttt ctttggtttg
    15961 cccaacatag tgatttttcg acgatctttg aaacatcggg tagctataaa agtattgaat
    16021 cattaaatta ttgggtagct agtttaccta aaattgagtg tcaaatgatt atacaaaaaa
    16081 atcccgaatt gaaggaagcc tggcatccgt tgtttggtga acgcattaat aaactagttt
    16141 ttattggtat gaagatggat caacaatgta ttaaggaaca acttgagggt tgtttaactg
    16201 attctcttaa tgcagataat ttccgaaaaa tttcaccatt tccatggaaa aagtctaata
    16261 tgtttagcca gatatcgtta atgaaatttt taagaaaagc tggtgtcaag ataggcgatg
    16321 tcataattat tggcattttg attattacct cttttattcc cttatttgta ttctctatag
    16381 aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc catcgttcga attgatggca
    16441 aggaaatgga tcgcttcgat ttggataaga tcaatcataa actcgtgaat tatcatcctg
    16501 cgaagggaca atataatatt gttgagatta aagatggaag aattcgagtt aaagaagata
    16561 atagtcctga tcaaatcgcg gttagaactg gatggatctc aaaacccggt caaacgagta
    16621 tttgtttacc ccataagtta gttattagta ttgagaaaaa agaaagcaca gattactaca
    16681 tttactaatc tggaaataga tgaggaggtt tttaataaga aaaagatatt attattaata
    16741 atagcagggg ctttattaag tgtgttaagc gcttgtggac aagaaaaaga agcggtgaca
    16801 acaggcacaa gtatctttaa cgggacctgc aatggtttag ttaacagtcg gttagattac
    16861 ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt caatagaata atcgagatac
    16921 tcacacggct agacagctat cttgtctagc cgtgttgttg ttctggcttt agtcagttgg
    16981 gtagtattct tagttttctt aaaatatttt cctcgatata aaaagattcg ttctcggtag
    17041 ttttgaaagt ttctatagcc ataggcaatt cgttttagta ctttgatatg gttattcaaa
    17101 cattctatag ggccgttgga atagggaacg cgcaacgctc gtttgatttc tgtttggtat
    17161 ttcttgaagg tcgtgaacac tggttggtag tgctctggca actccttgta gtcttctttt
    17221 aggagcgcga cgaattcagg aacatcttgt cttctgataa cggaaagaaa atcctggtag
    17281 acttcgtagc ctcgctttaa ggaatcatca taagataata gccgatcgac tatctctgct
    17341 tctgtcagat gatctcgaaa tgacggacgc cagtagcgat gctgaccttt caattcccac
    17401 gcattctttt gaagtagttt ccagtatttc tttaagtgct tcgaccgacg atcccctttt
    17461 ttaaaggtct tcattacttg aattcggtgg ttggtaaaag cccgaccaat gtgttggcta
    17521 atatgaaagc gatcggcgac gattcgggca ttcggaaaac actctttcac caatgtgcga
    17581 tagggtgtat agaaatctga aaccactaga cgcacttgtt cacggacttt tatagggtag
    17641 cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt ccacaatatc aatcagttga
    17701 tgggtcttgc cgtccatcat gacaaagctc atggctcccg agacattttt cactgatttg
    17761 aattcatcga aacacaccac ttctggtaac agctggctca aagaaatggt tttaggccga
    17821 aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt gcttcatacg tgcgatcaaa
    17881 gacatagaaa gaggttcgct taatagctca agaatggctt gtttgactct gcgagcaatg
    17941 gagcagcgac gatcactaac tgaatcttct gctaaaaaag tctttccaca gtgtttacat
    18001 ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt tactgacatc attcaataag
    18061 atggatactt ttttccagcc ctatttaatg atcgtttgtt cattttgata gtggcgatga
    18121 aagcattcct ccggttgata ggtcaatgtg ccatcaaaag caaaacatgt ttcgtcattg
    18181 atgcgctctt tgcgaaaggc atcctcatga aaggtaagat ttaggtctag aatatctagt
    18241 aactctttgg taaactctga ataggacatc ttgaaacact actttgaatg gtttttgtcg
    18301 actttattct aaaggatttt aaggtgtctg tttgcgttta ttagaaaaaa gaaaaagtat
    18361 tggcaacaga aggcgtcacc aacactagat attatagatc ccttattttc ttcgcttcaa
    18421 attgtcaagg agtcatcata aattttgtat ggattctttt gtttgggtag tagtcttcta
    18481 aataatcttg gattaacacc ttcatctcta ttttactttt atatttgttg gagtcgatta
    18541 attctcattt gattgatcga tgaaacgctt caattactgc attgtcaaca ggagtcccag
    18601 tttggctcac tgaatgtgtg atgtggtggt cgttcaacag ctcttgataa ataaaagacc
    18661 gatgctggct acctctatcg gaatgaatta ccgtaggcaa ttctccttaa taaatttgta
    18721 atggacggac gacaatctct ttttttatgt gtgaatctac catatagtaa cgtagaatac
    18781 gagttgctaa gtcaatatag gtggacaaat agaggcgacc gtcattacat ggaatgtaag
    18841 taatatccat cacccaaaca gcatcaattt ggttctgttc aaattatcga ttcaacattt
    18901 ttttaccatt tctttgattg ttttttctct accatgagaa gatttgcgtc gatgaaatcc
    18961 tcttgtgcat aaattcattt cacgcataag aacagctaca acacgtttat tgatttcgat
    19021 tccttcatcg taaagttttt gagtgatccc tggagatccg tatcgatctt tgtgttcgta
    19081 gaacactcgt cttcctcttt tagtcgttta ttttcctgtt caagaatgta gaaatctgct
    19141 ttatcaggta atctttttcc ttgtccagca aaagcatttt tcccgtatcg acgatattct
    19201 gaaacccatc gggaaattgt tggacgagag atattgtatt cttcagcaat tttcttggta
    19261 gttacttttt tctcaaggat atcctgtgaa atctttagtt taaatttacg atcatatgtt
    19321 tttctcaata gagttcattc cttccggtaa cgtaactgat agtcaagaaa agtgtatcat
    19381 agcccaactt agtatcaggg tttgtcaact aaactgtgga agttgataga taagagtttt
    19441 taacaaccaa aattctcttg actatttttg aaatcagaca aattttgatt ggacacatag
    19501 ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg cttttcaatt ctagaggaga
    19561 agtcctcgtt gaagtaccat agcccagttc tggattcgac ctcctgccca cttcatacgt
    19621 aaataaagta attttaggag tgagactgtg acaaaagtta tttttaagct aaaaacgaat
    19681 taaaaaacgt tgataattaa aatcattcgt tttagattct atgaatcatt atagaacgct
    19741 gtaaaatcga tactttataa ttataaagaa gatagtttca aatttattgt tcgaaatttg
    19801 aaactatctt tttacttatg tcacagactc ccttagttga gttattcttt aatttcgaat
    19861 ttcgagatag ctagatatat tgcaagcata cattcttgat aacaatcttg atcatatttt
    19921 ccagttctcc aggattgctt atgaatcaaa gagctaaatc ttttgagaat tgcctcaaca
    19981 gcttcttcat ttccagcctt tgctaaaatg aaatcttctt ttaaagaatt cataaatcta
    20041 ccttcttccc atggacttat aatctatttc tttgggaagg aggagaacga gcaaagaagc
    20101 atacaaaatt agttattatg cagatattaa accagcattt tttgaatttt tttgtaaagt
    20161 cgatgtttta aatttgtcgc accctgtctt gtaatcccca gaacctttcc aatttcttta
    20221 tcagttttat caaagataaa tttttcgata agaaaaattt gttcaggttc tttcaacaaa
    20281 ggaaggattt ttgctaattt tatatcttca acaaggagag tcacatttaa aacattaatc
    20341 ggttgtttaa attttatagt atctttagta aacaaatatg gtgggagttg atcaagttgt
    20401 tcatgttttt gaatttcttc ttgtttttta taataagtat tagcggtgtt caaaatgacc
    20461 ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt cgttatctga catattgcac
    20521 acctcctcca ttaagattta aagggaaaag aggcaaaaaa cacgaaaaga agctaatgca
    20581 attatgctgc gctgcgaata acttctataa gctcacatgc attgatagga ctgctgatgt
    20641 ttattttcaa aaatgttttt ttagttgata atggatagtt atctttatac ttagaaaaga
    20701 aaattcatat atcttcttaa atatcaaaca tgtccttatg gctaagaaaa tgtcttgagc
    20761 tttttacgta taataaatct tttctaaaac ctccttttgg ataatattct tattcgtgta
    20821 tagattgtat tttttatttc ttcccccttc ataattaaca actagaaaag agttcgaata
    20881 gacacgtatt tgaacgaaaa tttttattgg attcccaaat tggaatattt agaaacgaaa
    20941 aattcctatt atctgtatag tgcttagata ataacggatc ataataatta ttgtctttga
    21001 actagatata tgtaaatagt attcatcatg atgaaaaatg atgagttctc ggtgataaaa
    21061 tgtacttttt acggtggaat atacatgaaa gcgataagtg gatattttat aatgaagtaa
    21121 gttagcttat tttgacagag gaatagtatt ttagataacg tataaaatac tatttaatga
    21181 tgtagaaagg aaagagagaa acaaaagtgt tttcagataa agaagtctta ttaaagtctc
    21241 ttcatgtttg tttaaagtta cctatcgtgg tttttaattc cgaattcagt attataaaag
    21301 agtatcgctc agatagaact acttattttt tttatgatta taaaaagtta ctaactgata
    21361 atattaaaaa aactaaagat ttccatttct ttactggtga ttttaatgag atgtttcttc
    21421 tatacatgtt taaaaccaga tattacttat ttggtccatt tagagcaaat aatattgata
    21481 aagttttttt taaactaaaa atgaacaatt tgaatgttgc aatggctgat agagaacgac
    21541 tctataattc attgcaaaat ttgactttgt attctttagg agacattcga gatattctga
    21601 ttttagttca ttattttttt actggaaaaa tagaagatct attccatgaa ccgttaattg
    21661 aatatacaga aaatctaagt aaaactatcg agcaaatcaa aatagataat ctgttatcac
    21721 aaaattacga tccagaaata tatctgtttc tttatgaaaa taaaatactg gaatatgtta
    21781 aaaatggaga tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc
    21841 caagtgttag tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa
    21901 agttagcaca aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag
    21961 atgctttgat acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg
    22021 attctgggat tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc
    22081 ccttaatttc ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa
    22141 aggaaatagc taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg
    22201 aaatgcatat tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa
    22261 tgttgaaatc aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt
    22321 cacacttttc gagagtattc aaaaagtacg caggtgtatc tcctaagcaa tatcaattag
    22381 gtttagtgga caatttcaac aacattagct gagaggtttt attttattat gagactatat
    22441 catttacgta gtttggagga gttgaggaaa ttggatttta acaaaaaatt tttttgggaa
    22501 atttctgatt tgagttgttt cgtagaatct gagcgtctaa gtaatggtaa taatagtgtg
    22561 ttaaatgaat atgacaaagt agtaattaat aaagtaaaaa gtttatgtga agaagctaga
    22621 gaatctatca gtcatattaa tggtagcaat tttaaacaaa cattatttaa tctgattaag
    22681 ctagatgcta aaatatcaag ttatttattc tttttgatac atgatgaatt tatggattat
    22741 caaaaattgg atcagttaat tgaaagagaa agctgggaag attattacgt gggtctaact
    22801 ttttcagaaa agttaaacaa ctacaagtta atagattata agtatttgag tgaaagcaat
    22861 taattattca tgtagagtac tagacacttt aaatatggac aattcaatta taaagaaaaa
    22921 gaatcaataa tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta aaaacgagtg
    22981 aaaaaatgtt gataactcca atcattcgtt taagattcta ggaatcattc agaaatgctg
    23041 tgaaaccgat actttataat tataaagaag gtgatttcaa attccgaaga aggaatttga
    23101 agtcaccttt ttacttatgt cacagtctca ttcagtaatg cgggataagg ctccttattc
    23161 cgttatttga atactctgaa gtttaatcta agaaagggta atattttgaa aacatgcttc
    23221 atcgcataga aatgaactta atttcagaaa gaatacatac atatcttttt atatgttttc
    23281 agctgctgaa tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa tgtacttttt
    23341 ttgttgtaat gttcatgaaa ttctctaagt aaattagtta acataaggtt agtaagattc
    23401 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
    23461 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
    23521 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
    23581 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
    23641 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
    23701 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
    23761 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
    23821 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
    23881 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
    23941 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
    24001 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
    24061 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
    24121 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
    24181 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
    24241 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
    24301 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
    24361 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
    24421 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
    24481 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
    24541 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
    24601 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
    24661 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
    24721 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
    24781 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
    24841 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
    24901 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
    24961 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
    25021 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
    25081 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
    25141 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
    25201 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
    25261 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
    25321 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
    25381 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
    25441 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
    25501 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
    25561 ttcagatgtc tataataagg acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
    25621 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
    25681 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
    25741 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
    25801 cgcagg
//
LOCUS       DAFFFQ010000036        25694 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.036, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000036
VERSION     DAFFFQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.036"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            126..3653
                     /locus_tag="LQB69_001560"
     CDS             126..3653
                     /locus_tag="LQB69_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL3525728.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3650..7372
                     /gene="addA"
                     /locus_tag="LQB69_001561"
     CDS             3650..7372
                     /gene="addA"
                     /locus_tag="LQB69_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL3525729.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7441..7782
                     /locus_tag="LQB69_001562"
     CDS             7441..7782
                     /locus_tag="LQB69_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525730.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8091..9134
                     /gene="pheS"
                     /locus_tag="LQB69_001563"
     CDS             8091..9134
                     /gene="pheS"
                     /locus_tag="LQB69_001563"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL3525731.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9139..11559
                     /locus_tag="LQB69_001564"
     CDS             9139..11559
                     /locus_tag="LQB69_001564"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL3525732.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LQB69_001565"
     CDS             complement(11729..12739)
                     /gene="trpS"
                     /locus_tag="LQB69_001565"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL3525733.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12993..13730
                     /locus_tag="LQB69_001566"
     CDS             12993..13730
                     /locus_tag="LQB69_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3525734.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13744..14559
                     /locus_tag="LQB69_001567"
     CDS             13744..14559
                     /locus_tag="LQB69_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL3525735.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="LQB69_001568"
     CDS             14572..15219
                     /locus_tag="LQB69_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3525736.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="LQB69_001569"
     CDS             15235..15891
                     /locus_tag="LQB69_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL3525737.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="LQB69_001570"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="LQB69_001570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL3525738.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="LQB69_001571"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="LQB69_001571"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL3525739.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="LQB69_001572"
     CDS             18233..18751
                     /locus_tag="LQB69_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL3525740.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="LQB69_001573"
     CDS             18858..19358
                     /locus_tag="LQB69_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3525741.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19638..20558)
                     /locus_tag="LQB69_001574"
     CDS             complement(19638..20558)
                     /locus_tag="LQB69_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL3525742.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20723..21736
                     /locus_tag="LQB69_001575"
     CDS             20723..21736
                     /locus_tag="LQB69_001575"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL3525743.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LQB69_001576"
     CDS             complement(21846..22601)
                     /gene="fabI"
                     /locus_tag="LQB69_001576"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL3525744.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22821..23261
                     /gene="fabZ"
                     /locus_tag="LQB69_001577"
     CDS             22821..23261
                     /gene="fabZ"
                     /locus_tag="LQB69_001577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL3525745.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23376..24137)
                     /locus_tag="LQB69_001578"
     CDS             complement(23376..24137)
                     /locus_tag="LQB69_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3525746.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24326..25420
                     /locus_tag="LQB69_001579"
     CDS             24326..25420
                     /locus_tag="LQB69_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3525747.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25506..>25694)
                     /locus_tag="LQB69_001580"
     CDS             complement(25506..>25694)
                     /locus_tag="LQB69_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3525748.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         8509 a         4454 c         5416 g         7315 t
ORIGIN      
        1 gtatacttaa aaaagtagaa atatcagaac cttaaaagga tataatttga agaatataga
       61 acaaatgttc agtatttttt gatataataa aggagcaccg tatcatgtgt tgaaggagga
      121 aaagcatgtc tttacaattt attcttggaa acgggaaaaa agatcaccgt caagtcttga
      181 tcgacgaagc atccgcttgg ctcgaccagg atgcaagtaa ccaagtattt ttccttgttc
      241 ccaactacag caaattcgaa caggaacaag aaattttagc ggagatgcga agaagaagcg
      301 gaaaaaaaac tttcagcaca acaaacgttc aagtatttag tttttatcgt ctggcttggt
      361 atttgctgca acagacaact cttcttacag gaaatgaatt atcagaatca ggatctgcaa
      421 tgatcttgcg acagatttta gaaaaaaatg aggaagaatt aacaatcttt cgcggagaaa
      481 tcaataaaag agggtttatt cgtcaattac aagaactcta cagtgaattg cgtacaggaa
      541 atattcaacc agaagacctg atgcttttat ttggtgattc acctaatgca gaagaccagc
      601 aacttaaaat gaaagacttg aagctgattt tttcagctta tgacagcgaa ttgactcaac
      661 gtgcactaac aaatgaagat gcactatcta tactagcaaa ttatatgcag acacaagatt
      721 tcaacaacgt caaatttatt gtcagtggtt tttctcgaat caatggtcaa gagtatcaat
      781 tactgcaggc aatgatggaa aaggggcaat tggttgtcga tttattgctg gatcgtcctt
      841 atacagcgga tcttccagaa gtacttacgc tctttcatga gactggaaag ctttatttcc
      901 gtctttttca aagtgcaaga gaacggaaag tccctatatt agtggaccga tttgcaccag
      961 agcagactcg tccgacagag cttcaactgc tgcaaacaat gtgggaagag acatctagcc
     1021 aaaagaaagc ggcagtacac aaaatagggg gacaagagca tctccatttg tgggaagctg
     1081 aaaatccaac tgaagaagtc cgacaattag ctgttgaaat cagacgacta gtcagtgaag
     1141 aaaactaccg ttatcgtgat atccaagtgc tgacaaaaaa tatgtcttta tatggaaata
     1201 tccttcggcc gatctttcaa gaaatggcaa ttccttttta tatcgatgaa gaacaaatga
     1261 tggaaaatca tccgctgatc gaatggatca acagtctgtt cgctcttgac cgctattctt
     1321 atcggctaag tgatattatg cgttttttca aaactgaatt atttctgtcg acggaagcag
     1381 aaaccgaact tgatgaatgg aaacaagagc gagatgaatt caggaaaaaa ctggacatca
     1441 ctgaaaatgt agcacttgct tacaattatc aaggaactta ttggactcgt gaaaaagact
     1501 ggcagtttgt ggcgtatgat tttgaagcag aacagattga agacgtacag tatttggaag
     1561 aacagtcaaa tacgattaga cgattatttc agaaagctgt accagtattt ttgaaaaaaa
     1621 taaagaaaag caaaacagga caagaggccg caaatgtttt ttatcaattt ttgatccaaa
     1681 gtggcgtaca gcaacaatta ttgttttggc gtaatcagga agtagacaga ggaaacttag
     1741 aagcggcaag aaaccatgag cagacatggg gagcgctgat tgatctgttg gatgaatatg
     1801 tagtaattta tggtgaatcg gcttttgact ggtcactgtt ccaagaaatc attacaagtg
     1861 gtctagaaaa tctaagttat ggtaaaatcc ctaccgcaat cgaccaagtg cgaatcaatc
     1921 gtttggaatt agttcgacca aatcaagctg ccgtcacttt tgcattaggg ttgaatgatc
     1981 aagtgtttcc agaccgaaaa gagacaaaag gattgttgtc cagtgaagaa cgagaagtat
     2041 taaatcaggc cttgccagat ggacagtttt tattcgaccc atcaaaggaa agtatctctt
     2101 ttgagccttt ccaagcctat cttgtttttc tttccggaac agatcgatta tatcttagtt
     2161 atgcacaaag ctatgacaca gatggttcgc tgaagatgtc tccttatttg cgtagaatca
     2221 cagaatattt atctgttccc gtcgagagaa aagagcattt gacgatcgaa agcgacccta
     2281 attgttatgt agggacttac agaagcggaa tcaatacagt caataggatt tatcgattag
     2341 ctttagatga aaaaaaacct gtaccaaagc actggcaaaa gctcaaagaa ttagtactga
     2401 attcgtcatg gcgaaatttt gctttacaga tttttgaaag tcaggaacat ttaaatgttc
     2461 ctgtttcgat gccagaaaaa atggcggaag agctttatgg aaaagagatt tatacttcaa
     2521 tttcacgtat ggagaatttt tataattgtg aatacaaata cttcatgcaa tttggtttga
     2581 aattgaaaga acgaactgtt tatggtctaa ctccagcagc aactggtgat ttttatcatg
     2641 aatcattgga tcgtttcttc aagctactgt tttcaaacca attatcctta gtgaatatgt
     2701 ccgatgcgca gcgtagagac tttactgaaa aagtgctgca ggaagtattc ggagaacttc
     2761 ggtttgagat attggatagt tctgcaagaa tgaactatat ccgctaccag ttaggacaga
     2821 cgattcaaaa agtgacatgg gctttgcaaa aacagagcaa aaatacaggc atgcatcctc
     2881 ttcagacaga gattctattt ggtcagattg ctggagcaaa aggtatcaca ggtctggaat
     2941 taccgttgag taatggtgga aaactccatg ttcgagggaa aatcgatcga atcgatgtag
     3001 cttctgaaca agaagatact tggctaagcg tcgttgatta caaatctagc ggacgttcat
     3061 tcgatgtcac agaagcttat tatggcatgg ctatgcaatt attgacgtat ctggacgtag
     3121 cattgatgga tgcggtacgt ttgactggaa aggcggctgt taaaccagca ggatcatatt
     3181 atttacatgt tcataatcct gtattgacgg agacagatga tgtagagaag aatacattga
     3241 aaaaatttag ttatgatggc gtttttgtcg atgatcccgc tcttttagaa gtactagatc
     3301 attcactgga agcaaaagaa caatcactga tctatccagt gaaaaaaaat gcgaaagatg
     3361 aataccaaaa aggtagtttc agtaaagaca aatttttcac ggaagaagaa ctgcaactat
     3421 tcatggcgca taatcgggaa aatatgcggt tagctggaga tgcgattact tccggagaaa
     3481 tcaaactaaa tccagcttat aaagataaac agcggattgc ctgcgaattt tgtccattca
     3541 gaagcatctg tacgtttgat gtgatgctga aagaaaataa ttaccaccgt ctggagaaaa
     3601 tagataagga agaagcactg cgtagaatat tgaaaaggag tgaagaagag tgaatcaaat
     3661 tcctttaaaa ccgcaaaatg aacgattcac agatgatcag tggcaagcga tttttgatca
     3721 aggagataat ctattagtct ctgcgtctgc cggatcaggt aaaactacag tgcttgttcg
     3781 aagagtgatt gaaaaactaa aaatgggttt tgatatcgat gaattattga ttgtgacatt
     3841 tactgaagct gccgcaagag aaatgaaaga aagaattcaa gaggcattac aagaatccgt
     3901 caatagcgaa agtgaccctg tacgtagaca gcattttact aagcagttag ttttattgcc
     3961 cacagcgaat atctcaacct tacatgcttt ttgcctgaca gttattcgac gttattatta
     4021 cttaattgat attgatccag tctttcgcat gttaacagat gaaacagaaa cgatcttgat
     4081 gaaggaggat gtgtgggacg agctgagaga agcactttat gcagaaaatg atgaacgatt
     4141 tttccaatta acaatgaatt tttctaatga tcgttcagat gacggcttga cgaatctggt
     4201 gttttctctt tatgagtttg cccgtgccaa ccctgacccc caaaaatggc tagaacagtt
     4261 aagcgataat tatcgtttgc ctgaggggct agcgaaatct cgattatatc aaaaacaaat
     4321 ccgtccttta gttttggcag atatatacca atgtgtacag ttatatgaac agatgactca
     4381 gttagcacaa ggtgaaggat tagaaaaaat gcatgagcaa gtagctggag aacaacagca
     4441 gatcaagaat atctatgaag cgttctcgca agatcggtta gaagaagctt atgctggttt
     4501 ggaacaacta actttttcaa cttttaagag cagtcggaaa gcagaattga aagaaatttc
     4561 caatgaagtc aagggcatga gggataaagc aaaaaaactg attcagcaga taagtaaaag
     4621 ttattttcct gtttccccaa gccaaatgga agaattaacg gacaaagcac taccgttagt
     4681 cgaagaaatg acaaaagtca cgcaaagctt catggacggt ttcagtatgc gtaaacggga
     4741 aaaaggtgtg ctggatttca atgatctgga acatttagcg ttgcagattc tcacagaaaa
     4801 aacgaaagat gtatggctgc caagtgaagc ttcaaaacat tatcgaaaaa aattcaaaga
     4861 agtaatggta gatgaatatc aagacgtgaa ccaattacaa gaagccattt tatactggct
     4921 aagagaaccc gatgatacaa aaggaaatat gttcatggtt ggggatgtca aacaatccat
     4981 ctattctttc cgtttagcag atcctagttt atttatcggg aaatacgaga atttctcaaa
     5041 gaaagaaggc ggacgtcgaa tcgttttggc tgagaacttc cgctctagaa aagaagtcct
     5101 ttcttttacg aatcttattt ttgaacaact aatggatcca gcagtgggtc aaatcaatta
     5161 tgacgaagca gccaaactga tccaaggatt ttcagacttt ccagagaatg aacagtttga
     5221 gccggaaatc atgatctatg aaaaagaaca ggaagaatca gagattgaga ttccgacgga
     5281 tgatatactg gaagacaaaa cagaaggcga actttttatg acaggactaa aaattcgtca
     5341 attaattgat tcctccttta tgatttatga taaaaaatca aaaaaaagtc gtccgataga
     5401 gtacaaagac attgttctct tgacacctac aaagaagaac aacttaacga ttttagagat
     5461 tttcaaaaca ttggatattc cattagagat gaatgatgct caaaactatt ttcaagcgac
     5521 agaaattcgc acgatgatct cgctgctaca attaatcgat aatccctatc aagatattcc
     5581 gcttgcggct gtattgcgct caccgatcgt gggtctgatt gaaccagaat tagccagcat
     5641 tcgattagct aaccgtgcac atacttacta tgatgctgtg cttgcttatc aagcatcaaa
     5701 cgaagatgaa ttagcagcta agctggaaca ttttggcaag caactagagc attggcgaga
     5761 acttgctcgc cgttcttcga tcactgactt gttatgggat atctattatg agacgggata
     5821 tctagagtat gtggtcggtc tgccagcagg ggcacagaga caagccaatc tttatgcatt
     5881 ggttgatcgt gccaaagctt acgagcaaag ttctttcaga ggattatacc agttcgtgcg
     5941 tttcattgaa aaaatgcaag aaaaagataa agacttagca gaaccagtca tttcaataga
     6001 agataatgct gttcgcgtta tgacgatcca tgcaagtaaa ggcttggaat tcccagttgt
     6061 atttttgtta gatatgacaa aggagttcaa cttacaagac ttgcgcaatc ggtatgcttt
     6121 tgaagaaaaa cttggagcag gaattcgcta tatggatcct gaaacacgag tgttatatga
     6181 tacgttaccg tttcaagcca tcaaattggc aaagcaaaac aaattgttgt cggaagaaat
     6241 gcgtaaattg tatgtaggat tgactcgggc agaacaaaag cttttcattg tcggttccta
     6301 taaaaataaa gagcaaatga tccaaacgtg gtctgaagca gctgatcatg aggagttagt
     6361 ttttgatcct gcgctacgtt tgaagggcag aagcagcttg atgaattgga ttggttacgg
     6421 attgatccgt catcctgaaa tgcagaaata cttagaggaa gaaatttcga cttctcttct
     6481 tcaacattca aatgcacagt tttctatttc atggatgaat cagcaatcga tcattgaaca
     6541 aagacagttg cttgctgaaa aagaactggt taacttagat cagcagatga aagaagatga
     6601 aactttatta gcagattcgt tacaaaaacg tctggcatat gaatatcctt atcaagcttc
     6661 ttcacaaacc acaagttatc aatctgtatc tgaaatcaaa cgattattcg aagatccaga
     6721 tgatacacaa gaatcacgtt tgactctgga aagcagtcag aacaaagcag catcccgtca
     6781 atttcgttat acgcaagaac aactagcaga accaaaattt ttgcaaaaag atcggcaagt
     6841 gtcagcagct gctgttggaa ctgctacgca tgctttgtta caattactgc cattagagat
     6901 gccaactaca gagagtatcc atcaaaaatt acaggaattg gtaaggaaac ggttagtaga
     6961 cgaaaaagtc gcgaaaaaag tagatgtttc ttctattata tggttctttc aaacagaact
     7021 tggtcaacag ctgattgcca acaaagaaaa cgtaaaacga gaacaacctt tttccatgct
     7081 gcttcctgct gacgaagtat ttcaagacta tccaaatcaa gaagatgaac tattaatcca
     7141 cggaatcgtc gatggatacc tagaagaaaa agatcatctc aatatctatg attttaaaac
     7201 agattttatt ttacatccgg atgatccagc agaaatagat gcaatcgtcc aaaaatatca
     7261 aggacagcta agactttacc aacaagcaat gtcagaagct ttgaataagc cagtagaaaa
     7321 tgttttccta attttgcttc gagtgaaaca aattataaac attaacaaat aattataatt
     7381 gagcaaactt gacttatgtt ttgtaaggat tatgctatac tctgggaaga ggtgagtatg
     7441 atggagctaa cacagacaga attcgtttta tgccctaaat ttgaaaaaac attctctatt
     7501 ttaggaaaaa aatggaacgg attgattatc gatgtactac tcgaaaacgg tccgcaacgc
     7561 tttgttgagc ttgcggcaaa aatcccgatg gtcagtgatc gagtattagt cgaacgcttg
     7621 aaggaattag aaaaagaagg aatagtcact cgaaactttg cctgtagcga aaagagccgt
     7681 tcagaatatc tcttgactga aaaaggagca gacttgcaaa aagcgatgcg tcaaatacaa
     7741 gtttggagcg aaaaatggat cacagaggaa gaatgcggtt gattttgtaa aaaaaatctt
     7801 gtaaactata gtccatacga ataagtaaag aacgatgacg aaaaagagta gtttggctaa
     7861 accagttcag ggagatctgt cgcagactga aagcagatcc tgtgtttgaa aaatgaagtt
     7921 cactttccga gttcgtctga taaaatcaga ccggcaattg tcgttaacga agtaagagtg
     7981 cggtgtaagt catttatccg caaattagga tggtaacgcg aagacctcgt tccttaacag
     8041 gaatgaggtc tttttgtttt gtatttggaa tttaaaggag gaacaagcac atgtctctac
     8101 aagcacaatt agagagcttg aaagaagcaa cgctcgtcaa aatcacagcg gcacaagatt
     8161 taaatgaatt aaatcaaata cgtgttgaaa cgttaggaaa aaaaggaccg atcacagaag
     8221 tattacgtgg gatgaaagat ctaagtccag aagagcgacc aaaagtcgga agtttcgcaa
     8281 atgagatccg agatcttttg acagaagaga tcgagcgaaa aaaacaagta ttagaagaag
     8341 aagcgttaaa tgcagctttg gcaaaagaaa caatagacgt gacactacct ggtagaaaag
     8401 taaaacatgg tacacgtcat atcgtgacac aaatcatgga agaaattgaa gatatttttg
     8461 tgggtatggg ctatcagatc gtagaaggat atgaagtaga atctgatcat tacaatttcg
     8521 aacggatgaa tctgccaaaa gatcatccag caagggacat gcaagatact ttctatattt
     8581 cagacgagat attgattcgg acacatactt caccagtcca agcacggaca atggaaaaac
     8641 atgatttctc taagggtgct ctacgaatga tctcgccagg gaaagtcttc cgcagagata
     8701 cagatgatgc gacccacagt catcagttcc atcaaattga agggctagtt gttgataaaa
     8761 acatcacaat gggcgacctt aaaggaacat tagaagtcgt aatgaaaaaa atgtttgggg
     8821 aagatcgtga aattcgtttg cgtccaagtt atttcccatt tacggaacca tcagtagaag
     8881 tagatgtcag ctgtttcaaa tgtggtggtg ccggttgtaa cgtatgtaaa tacactggat
     8941 ggatcgaaat tttaggagct ggcatggtac acccgaatgt gttgaagatg tcaggaatcg
     9001 atccagaaga atattcaggc tttgctttcg gccttggacc agaccgcgta gctatgttgc
     9061 ggtatggagt gaatgatatc cgtaatttct atcaaaatga cttacgtttc ttaagtcagt
     9121 tcaaggggga gtaagaaaat gttagtttca tataaatggc tcaatcgtta tgtagattta
     9181 tcgaatgtaa caccaaaaga attagcggat aagatgtctg tgacaggaat cgaagtagag
     9241 ggagtaacgg ttccagaaga aggcttgaaa aaaatcgttg tcggtgaagt caaggaatgc
     9301 gtaccacatc cagattctga tcacctttct atttgtcaag tcgatattgg cgaagacgaa
     9361 ctgtctcaaa tcgtttgtgg agcgcctaat gtgaaggttg gaatcaaagt gattgttgca
     9421 cttcctaact cacgtatcgc tggtaatgta aaaatcaaaa aaggaaaaat gcgtggccaa
     9481 gtatcaaatg gcatgatttg ctcattacaa gaaattggct attcagatag tgttataccg
     9541 aaagaatacg ctgaaggtat ctattatatg ccacaagaag ctgtaaatgg cgatccagtt
     9601 tttccttatt tggatatgga tgattcgatt attgaattat ctatcacacc gaatcgtgcg
     9661 gatgcattgt caatgcgtgg cgtagcttat gaagttgggg caatttatcg tcagactcct
     9721 acattcgatg acgaaaagct ggtagaatcc tcacgtcctg ctagcgataa aattcaagtg
     9781 gaagttgaag ataaagaagc agttcctgct tatcaaatca gaatcattga aggagtaaga
     9841 atccagccaa gtcctcaatg gctgcaaaat cttttgatga acgaaggcat tcgtccaatc
     9901 aacaatgtag tagatgtgac gaactatatt ttgcttttat tcggacaacc actacatgca
     9961 tttgactacg acaaattgaa atcaaaagag atctttgtcc gccgtgcaaa agaaaatgaa
    10021 acattagtaa cactagatgg agaatctcgg gaattgacaa ctgacaatct cgtcattact
    10081 aacggcaaag aaccagttgc tttagctggt gtcatgggag ggttagactc agagatcacg
    10141 aatgaaacaa caacagttgc actagaatct gctttatttg atccaatttc gattcgtcgt
    10201 acagcaaaac aatttaattt gcgtagtgaa tcctctgcac gttttgaaaa aggaatcaac
    10261 catgcgactg ttggagaagc cagtgaggtt gctgcagcaa tgatcgtttc tcttgcgggt
    10321 ggggaagttc tacaaggcgc ggtcaaaggc tcagagatca aagcagaaga cgtatcagtg
    10381 aagatttcac ttgaacgcat caatcaatat ttaggaacat ctcttgtcgt taaagaagta
    10441 aatgagattt ttgaagcact tggatttagc tatgaagaaa aggagtatgt ttattccgta
    10501 gtagtaccac caagacgctg ggatatccaa atcgaagcag atattatcga agaagttgct
    10561 cgtatttatg gatacgacca gttgccatct acacttccaa gtggagaaac ggttgctgga
    10621 agcttgtcaa aagaacaaga aactactcgt aaaattcgta caatctttga aggaagcgga
    10681 ttgagcgaag caatcagtta tgcattgaca acagaagaaa aatcaagaca gtttgttata
    10741 aatgaaagta atattacacg tttggactgg ccaatgagcg aagaacgttc tgtattacga
    10801 atgaatttga tcagcggact attagatgat atcagttata acactgcccg aaaaaataca
    10861 gagattgctt tatatgaaat tggtcgggtg ttcttccaag aaaatgatcc acgtacacat
    10921 ctgccgcaag aagtaaaaca tgcagcaatc gctatttctg gtatttggga agaaaaagat
    10981 tggcagacga aagctcaaaa tgtagacttt ttcaccatca agggtctttt agaaaactta
    11041 tttgaacaac taggcatcac tgaagatgtc cattatcaaa cagtgacaga aatgaaggaa
    11101 atgcatcctg gaagaactgc tgcgatttac cttggcgaaa aatttattgg ttttgtcggt
    11161 caagtgcatc caacgattac aaaaagttat gatattccag aaacatatgt tgctgagttt
    11221 gatttatctg ctatagtgga agctgctcaa aaagggatcg ttttcgaagc agtgacaaaa
    11281 ttcccagcag tcagccggga catcgctttg cttgtaaaag aaactgtgac aaatcaggaa
    11341 ttaacagaag ttatccgttc tgctgccgga agatttttaa cagatatcca attatttgat
    11401 gtctatcaag gagaaaacat tgaaaaagga cataaatcca tggcgtatag cctgacattc
    11461 gtcaacccag aagcaacatt gacagatgaa gaaatcaata aagcaatgga aaaagtcgaa
    11521 aaagcgttgg tagaaaacct agaggcaagt attcgctaag aaggaaaata tagtaagtaa
    11581 cgatcaagta gcttttgaag accatacatt tgaaaaaaag atgtgacaca acttcgtcac
    11641 atctcttttt ttgattttaa aaaaggaata gcacagagac gagataaaaa aacaggcctt
    11701 tacagcctgc ttttttagag taaatgaatt aatggaagta attgattccc attgctgatt
    11761 ttacttcaga aagtgtttgt gcagctactt tagcagcttc ttcactacct ttacgtaaga
    11821 tttccataac agctgaagga tctttttcca attcttctcg gcgcgcacgg atcggtgcaa
    11881 attcagcttc caatacatca atgaggtatc gtttgatctt tacatcacca agtccgccgc
    11941 ggcggtaatg agctttcatt tcttcaattg cttctttatc tgttccgaat acatccaagt
    12001 aggtgaagac catattccct tctacttgtc caggatcttg tacatggatg tggtttggat
    12061 cagtatacat actcatgact tttttctgta agacatcggc aggatcggag atataaatac
    12121 cgtttcctaa cgatttactc attttgccat ttccatcgat tccaggtaat ctacccattc
    12181 cttttggtgg gaatacgcct tttggttcca ccaagacttc accatatgta tgattgaagc
    12241 tttgtacgat ttcctgtgtc tgctctagca ttggtttttg atcctcacca acaggtacaa
    12301 gattcgcttt gaatgcagtg atatctgctg cttgagaaac aggatagata aagaatcctg
    12361 taggcacgct ttcaccgaat tttttttgtt cgatttctgt tttgacagtc ggattgcgcc
    12421 gaacgcgtcc tacgctgact aagttcaaat aatacatggt taattcagct aattccggaa
    12481 tttgtgattg gataaataaa gtagattttg caggatctaa tcccacggct aaatagtcta
    12541 aagcaacttg caagacattt gatgaaacct tctcaggatt tttagcatta tctgtcagtg
    12601 cttgcatgtc agcaatcatg acaaacagtt ggttgtcttc atctgcctgc atagccactc
    12661 gggtttttag agaaccgacg taatgcccta aatgcagctt cccagttggt cgatctcccg
    12721 ttaaaataat atttttcaaa aaagacacct ctttacgatt tgtaagtctg agcacttaat
    12781 atcttaccgt ttttcctgaa aagaaacaac agttcctaag gaaaaaacaa ggaaagagga
    12841 taaaataact aatagattag aaatggaaga aatatttatg ggagaataaa cgttctaaga
    12901 aactttacaa attactaaac tatcagatat aatgtatttt attatacatc tttaaaagaa
    12961 atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt gatcacgtgg
    13021 ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc gagaaaggag
    13081 aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt cgctgtatca
    13141 atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac ttgcatgata
    13201 aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa catttcaatt
    13261 tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa gtgttgaagc
    13321 aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc aatatgttag
    13381 ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg gtggcaatcg
    13441 ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact tctgctttgg
    13501 atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat gacggtatgt
    13561 caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat cgtgttatct
    13621 tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt ttcgaaaatc
    13681 caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga taggaggaaa
    13741 aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatta ttttccgaca gcaattggac tgttcggaat cgtgaaacca
    19681 gaagctgtca attctttgac ataggagctc aagaactctt cttttaaggc atattgtttt
    19741 ccgttcaatg cgtagcagac cgtacgaatc gcaaaattgc tgtttccaag atccactaat
    19801 ccaaagatag aaggttcagt ttggagctct tcttggtatt tttcagctaa tgtacgattt
    19861 actcgatcaa tgatctcata gattttgtca tatccttctt caggaacaat ccgaatatct
    19921 aaaagtactt gcatatttgc acgagagaga ttgctgatcg tggtgatgtt tcgattcggg
    19981 atgaaatgga cagtaccatc cgttgctttc agctgaaggg tacggatacc aacagatacg
    20041 acggttcctt caatcgttaa gtttgctagc ttgatgtagt ccccaacatc catctgttgt
    20101 tccatgataa taaaaaatcc ggtaataaca tcactcatga acccttgggc accaagaccg
    20161 attgccacac ctgcaattcc ggcaccagcc aaaagtgatc cgatcggcac accgatagca
    20221 gataaaattg catagataaa aaagaaacca agggtataat gaaaaatagt atttaaaaga
    20281 ctatgtaaag tctttaagcg gctctcgttt agaatggctt ttttagaata ctgattaaaa
    20341 gacttatcaa tcagatattt tcccacacgg tgaagaatcc aaaaaaggac gatcaaaaaa
    20401 atcagatata aacttttttg gataaaggca gccaaaatgg cgtcccaatc aatcccgtcc
    20461 caaaaccgct gaaacgcatt tactttgctg acagcttgat ctgtcaatgt ctcgccagaa
    20521 gagtctgtcg taccggcagt tgctaataat gaaaacataa aatcctcttt tctaaaattt
    20581 gattctttct gtattgtacc aagaagtttt caaaaaagga aatagcaaaa ataactataa
    20641 tgaaggaaaa aagctgaaaa taaaatgaca aataatatgg ataatgataa aatgtggaga
    20701 aaataacgaa ggtggtaagt atatgcaaat cgattggaac aatctaggtt tttcttatat
    20761 caaaactcct tggcgtttta ttgccaaatg gaaagacgga caatgggaag aaggagaatt
    20821 gacagaagat aattacctga cgattcatga aggctctcca gcgcttcatt atggtcagca
    20881 atgttttgaa ggtctaaaag cttatcgtcg aaaagacggc aaggtcaatc tctttcgacc
    20941 tgaacaaaat agtcgacgga tgaatcaaag tgctagacga ttgctgatgc caatggtccc
    21001 tgaaaagatg ttcgttgatg ctgtcaagca agtggtaaaa gcaaatgaag attttgtgcc
    21061 gccctatgga acaggcgcta cattatactt gcgcccttta ctgattgggg tcggtgaaaa
    21121 cattggtgtc catccagctc cagaatattt atttgtcgtc ttctgtacgc cagtaggagc
    21181 gtatttcaaa ggtggactaa aaccaacgaa ttttattgta tctgattacg atcgtgctgc
    21241 tcctaagggg actggtgcag caaaagtcgg cggaaattac gcagcgagtt tacttccagg
    21301 aacagaagca aaagaactgg attactcaga ttgtgtttat cttgatccag ccacacatac
    21361 gaaaatcgaa gaagtaggtg cagcaaattt ctttggaatt acaaaagacg gaaaattcat
    21421 cactccgcaa tcgccctcta tcttaccaag tatcacgaaa tattccttgt tgcagttagc
    21481 tgaagaacga ctaggactgc aagcaattga aggagatatt ttcattgatg atctagatca
    21541 atttgcagaa gctggtgcat gtggtacagc ggctgttatt tctccaatcg gtgggattta
    21601 tcacaataac aatcttcatg tattttatag tgaaacggaa gtaggaccag taacaaaacg
    21661 gttgtatgat gaattgacag gtattcaatt tggagacata gaagcaccag aaggctggat
    21721 tgaagtcgtg gaataatttc aaagtaatga gttgaaaaaa acgtaaaaag aaaaaatcag
    21781 gaagagactt taaaaagttt tttcctgatt tttatatgtt ttactttaaa gaatgcaaca
    21841 aaagtttatg aaagatggac acctttgtca acgtagataa tatcaccgat gattccgctg
    21901 gaaagtgggc tgattaagaa agcacaagca ttaccgactt cttcgatcgt cacaccttgc
    21961 tgatcaggcg tacgagattc agataactgg atcagttttt gatagtcttt aacacctgtg
    22021 acagctaatg ttttgattgc cccagctgag attccattga cccgaattcc tttaggcgag
    22081 aactcagctg ccaaataacg gatagcagct tcaagagaag ctttcgcaat acccatcatg
    22141 ttataattcg ggattgcgcg ttctgaacct agataagtca tgctaacgat cccgctgttt
    22201 ggtgcaagaa acggttgtgc gtattttgat acagcaatta aagagtagct gctgatatct
    22261 tgagctaatt gatagccgtc tctggaaata tcagacacat ttccggacaa ttcttctttg
    22321 ttggcgtaag caactgcatg gacaaggccg tggatctccc cagcatattc accgatggca
    22381 ttcattgctt tttcgatgct ttcgtcgcta gaaacatcac attctacagt gaatgcttct
    22441 tcgccgacca atttgactaa tgattttttc attcgttcat tttggtaagt atagataatt
    22501 tctgcccctt gtttcaacat tgcttcagca caaccccaag caatgctttt tttgttagcg
    22561 acgcccataa tgacgatttt ttttccttct agaaatgaca taatgactct cctttgtttc
    22621 tgataaaaat ttgatagtcg taaaaaaata gagaataata gctaaaataa cttcttgatt
    22681 tcttaattat tagtcaaaat gttttgattg tcaaactatt ttttcttaaa ttaaaaaaag
    22741 attgctaaat atttaggaat atgatacttt gatagtcaaa gtaatgaaaa tggcaattgc
    22801 ctgaaagaag gagtttaatt atgtcttacg tattatctgc tactgaagta atggaattga
    22861 ttcctaatcg ttatcctatt tgttatattg attatgtaga tactttagaa ccaggaaaaa
    22921 agatcgttgc gacaaaaaat gtaacaatca atgaagattt tttccaaggt cactttcctg
    22981 acaatccagt gatgccgggt gcgttgattc tagaaacact ggcacaagca ggttcgatac
    23041 ttatcctaaa atcagaagag tttctaggaa aaagagctta tatcggtggg atcaataagg
    23101 ccaaattccg tcaaaaagtt gttccaggag atgtgctgaa atgtcatttt gaaatcacca
    23161 aaatgaaggg atcagtcggt acagcgttat ctgcagcata tgtagatgat aaaaaagtct
    23221 gtgagtgcga gttcacgttc atcgtcaacc aaaatgcttg actataaaac ctgatatagc
    23281 aaaacaaaag gaagcttact tgctttttat gtaaaaagaa gacgcctgaa tcctagcata
    23341 cgttgtcatc tatgcaggat tcagacgtta tttttttatt tttgttttgc ttcttgcagt
    23401 atattgattt tatcgatttt tacttcttct tttggtttgt ctgaactatc tgtttcgata
    23461 tttgcaatag catccacgac atccattcct tcaatcactt ggccgaagac agtataatgg
    23521 ccatctagtg aaggataacc gccgttttta taagcgtcga tgattgcttg aggatagtcg
    23581 tcttttaaaa gaccatcatg catatcatct gtattttgaa cgataaagaa ctggctgccg
    23641 ttactgttag gatcttgtga gcgagccata gataacgcac ctcttatatt ataaaggtgg
    23701 ttgttgatct ctgtttcaaa tccttctccc caaatgcttt cccctccagt gccatcgcct
    23761 ttaggatcgc caccttggat catgaaatct ttgatgacgc gatggaaagt cagtccgttg
    23821 tagtaaccgt cttttgcatg tgtcataaag ttttcaaccg cttttggtgc gtgttcggga
    23881 aataatttga ttttgattgt gcctttattc gtcaccattt cgaccagatc ttcattttcg
    23941 gcgacatctt tcgtcaattg tggtaattcc aatgcgttca aatcgacgct actggcagtc
    24001 gtgctttccg cagtagatgt actttttgaa ctttctgtag acgaactaga agaagtcgtg
    24061 ttgtttgcag aacaaccagc agcaaacagc aatgctgctg ctaaactaat acttaaaata
    24121 actttatttt tcttcataac tgttcctttc caatttacta ttttgttatc tttaatgata
    24181 acaaaataga gggaaaatac ctacttaaat tattggaatt tatcatttat tttaaaagaa
    24241 aaagaaaagg tgtagaccat ttactttgtg tttgctataa tgtaagtgga tacaaaaacg
    24301 aaaagatgca ggaggaaatg gtaatatggg aaaattagga atctcgattt atccagaacg
    24361 gtcaacattt gaaaaggaca aagcgtactt ggatttagct cataagtatg ggtataaacg
    24421 ggtttttacc agtttgctgc agatcaatga tgataaagaa aaagttcttt cagaatttaa
    24481 agaagtagtt gattacgcga atagcctagg catggaagta atggttgata tcaatccagc
    24541 gttatttgaa cagttggaga tttcttacga tgacttatct tttttccata agatgggtgc
    24601 ctacggggtc cgcttagata tcggattcac aggagcagaa gaagcaaaaa tgacgcgcaa
    24661 tccttttggt atcaaaatcg agatcaatat gagttcgggc acgaattacg ttgacaacat
    24721 catgagttat tcgccaaaca cggataattt attaggttct cataattttt atcctcatcg
    24781 atactctggt ttaggatacg aacactttgt tttttgttca gaaaaattca gaaaatataa
    24841 tttgaatacg atggcattcg tcaattcgca atctgctgag tttggccctt ggcctacgca
    24901 agatggcttg tgcacgctgg aagaccaccg agatcttgaa attgcgacac aagtgaagca
    24961 tttaatcctt acaggattga ttgatgatat ttctattgga aatgcgtacg caagtgaaga
    25021 agaattgaaa gaaatggcag aagcatttaa cgcggattat ccaacattga aagtagatac
    25081 ggaagaaggt attacagaaa acgaacgaat ctgcttgttt gataaccttc atagttatcg
    25141 aggggatcgc tcagaatata tccttcgttc aacgatgact cgtgtatact acaaagataa
    25201 agatttcccg cctcataata cgcgagatat gcatcatggg gatgtactga ttgacaatga
    25261 agggtacggt caatacaaag gagaaacaca aatcgccttg aaggatatga aaaacgacgg
    25321 acgtgtcaat gttgtaggaa gaatctccga tgacgaatta ttcctccttg actttttgaa
    25381 accatggtca agctttaaat taattgaaaa taaaaaatag ataattagtt attgcagaaa
    25441 taatccgcta gaaatagcca gttatttctg cttttttatt ttgttttttt ataggttatg
    25501 atttgtcaaa ttaagctaga ataaaatgct tcttgtacat agctcaaaaa tatttcgatt
    25561 ggtgttctgt aattcaatga ttttcttgga atatgattac gttgattgct gacactggaa
    25621 ataaatgtct gattcacttt tctaaaatcc attgatttcg gcagtccatt acgacgcaga
    25681 atcccgttag aatt
//
LOCUS       DAFFFQ010000037        23803 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000037
VERSION     DAFFFQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.037"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..1250)
                     /locus_tag="LQB69_001581"
     CDS             complement(<1..1250)
                     /locus_tag="LQB69_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HBL3525749.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYL"
     gene            1523..3994
                     /locus_tag="LQB69_001582"
     CDS             1523..3994
                     /locus_tag="LQB69_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL3525750.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4097..5833)
                     /locus_tag="LQB69_001583"
     CDS             complement(4097..5833)
                     /locus_tag="LQB69_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL3525751.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5830..6534)
                     /locus_tag="LQB69_001584"
     CDS             complement(5830..6534)
                     /locus_tag="LQB69_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3525752.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDLHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6665..7168
                     /locus_tag="LQB69_001585"
     CDS             6665..7168
                     /locus_tag="LQB69_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL3525753.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7128..7466
                     /locus_tag="LQB69_001586"
     CDS             7128..7466
                     /locus_tag="LQB69_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL3525754.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7487..8905
                     /gene="ffh"
                     /locus_tag="LQB69_001587"
     CDS             7487..8905
                     /gene="ffh"
                     /locus_tag="LQB69_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL3525755.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9017..9595
                     /locus_tag="LQB69_001588"
     CDS             9017..9595
                     /locus_tag="LQB69_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL3525756.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9599..10120
                     /locus_tag="LQB69_001589"
     CDS             9599..10120
                     /locus_tag="LQB69_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3525757.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10199..10753)
                     /locus_tag="LQB69_001590"
     CDS             complement(10199..10753)
                     /locus_tag="LQB69_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL3525758.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11006..11281
                     /gene="rpsP"
                     /locus_tag="LQB69_001591"
     CDS             11006..11281
                     /gene="rpsP"
                     /locus_tag="LQB69_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL3525759.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11293..11544
                     /locus_tag="LQB69_001592"
     CDS             11293..11544
                     /locus_tag="LQB69_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL3525760.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11669..12460
                     /locus_tag="LQB69_001593"
     CDS             11669..12460
                     /locus_tag="LQB69_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525761.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12630..13151
                     /gene="rimM"
                     /locus_tag="LQB69_001594"
     CDS             12630..13151
                     /gene="rimM"
                     /locus_tag="LQB69_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL3525762.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13141..13902
                     /gene="trmD"
                     /locus_tag="LQB69_001595"
     CDS             13141..13902
                     /gene="trmD"
                     /locus_tag="LQB69_001595"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL3525763.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14038..14385
                     /gene="rplS"
                     /locus_tag="LQB69_001596"
     CDS             14038..14385
                     /gene="rplS"
                     /locus_tag="LQB69_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL3525764.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14758..15420
                     /locus_tag="LQB69_001597"
     CDS             14758..15420
                     /locus_tag="LQB69_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525765.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            15499..18726
                     /gene="fss3"
                     /locus_tag="LQB69_001598"
     CDS             15499..18726
                     /gene="fss3"
                     /locus_tag="LQB69_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL3525766.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18840..19154
                     /locus_tag="LQB69_001599"
     CDS             18840..19154
                     /locus_tag="LQB69_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3525767.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            19167..19541
                     /locus_tag="LQB69_001600"
     CDS             19167..19541
                     /locus_tag="LQB69_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3525768.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            19551..19895
                     /locus_tag="LQB69_001601"
     CDS             19551..19895
                     /locus_tag="LQB69_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL3525769.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19966..21111
                     /locus_tag="LQB69_001602"
     CDS             19966..21111
                     /locus_tag="LQB69_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3525770.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQCATCF"
     gene            21826..23685
                     /gene="ltrA"
                     /locus_tag="LQB69_001603"
     CDS             21826..23685
                     /gene="ltrA"
                     /locus_tag="LQB69_001603"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3525771.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         8092 a         4211 c         4937 g         6563 t
ORIGIN      
        1 tgtaaataga tccgtgcccg aaagttataa aagttgcgat aaccaaacgc aattcgttta
       61 atcagcttga tcttgttatt cagcccctca gtgacgccgt ttgagtggga caccttaaag
      121 gcatttgaaa tacccgattg gtagcgatgg aagatcgtga atttcttttt aaacgactga
      181 ggtaacgtag atgaaattgt tttaagggtt tcttgaaagc cttccgtacc tcgtttaacg
      241 aaataatagc gtaataattg acaaaaatga tacgcagttc gcaattcttc gttgtagctt
      301 aacagctcat caacgatttc tgtttgacta attggtcgtt taaacagtcg atgataatgc
      361 cgtttgagtg gctccaatgt cgtggaatct ttgagtaaga gtttccaata tcgcttgagc
      421 cgacggtact tcttctgatt ttcagagtgt gaggttcgaa aacgattcat gatctggaca
      481 cgtaattgat tgaagctgcg attgatgtgt tggacaatat gaaagcggtc agttacgatc
      541 tccgcatttg gaaagactgt tttaagcaac tgacaatagc tggcattcat gtccatcact
      601 aggtacttga cctttaaacg ggcctttcta ggaaagcgca ggaagtattg agttagtttg
      661 taaagtctgc gatcttcaag gatcgtaaat agctgattgg ttgtcccatc aacacaaata
      721 aaactcatgg aaccagaaac agaattcatt gatttgaact catccataca caacaccgtc
      781 ggaaggaagt tcatacgtgg ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc
      841 gtcccatccg agacgaagta cttcttagcg attagtttac gtgattgatt ttcgtacagt
      901 tccatgagaa tcacctgctt cagttccttt gttagttgat gatgttcatc aactaagtcc
      961 gttttcgcag aaaaggtcct tccacaatca tggcagagaa aacgggtacg ttttaattct
     1021 aaaatagtta acttttcttt gactttcaac atctgagtgc gtgtcgtata ggaaccgttg
     1081 cggataatac gaaccgatcg acagtgttca caataggaag aagagtcatc caattttcct
     1141 ttaataaact gcgcttgaac gcctttatat tctttttcaa tcagccattc ttcatcaaaa
     1201 aataaatgtt tatctgttaa tccgagtaat tttctagtat gattatccat tgagagactc
     1261 ctttggtagg atgacgtttt ggtcgactaa attttaccaa aagtgagtct ctttttgagt
     1321 tgaaacgaag aaaaagtgtt agtagaaaat cgccttcgat tttccaccaa cacaaaatat
     1381 tatagaaccc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
     1441 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
     1501 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
     1561 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
     1621 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
     1681 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
     1741 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
     1801 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
     1861 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga
     1921 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggata
     1981 tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt
     2041 gacaggcgaa gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt
     2101 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
     2161 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
     2221 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
     2281 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     2341 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     2401 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     2461 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     2521 gtctgttcaa aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     2581 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     2641 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     2701 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     2761 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     2821 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     2881 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     2941 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     3001 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     3061 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     3121 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa
     3181 tacagtagct atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg
     3241 cagtatagcg atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact
     3301 tgattctgac ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa
     3361 tattgagcga tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact
     3421 gttttcagtg atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag
     3481 tttgtttact atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat
     3541 cagagggaaa ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat
     3601 gatcgtagtc ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt
     3661 ttctacagca tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg
     3721 ttcaccgctg aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt
     3781 ttcggtcatc tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt
     3841 gctgttgagc attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt
     3901 agaatatttg tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa
     3961 aaaatttcta tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa
     4021 aaagaacaac cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac
     4081 gagcagttgc tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa
     4141 gatgactgtc tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta
     4201 accctaatcc ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct
     4261 caaagatccg ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg
     4321 atagatcatc tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga
     4381 cagcattttc aattagattt ttgataattg gataaaggat atctgtttta gtagtaaatg
     4441 gttcgttctt tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat
     4501 tttgaacgat ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct
     4561 cgccatcttt tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt
     4621 tttgcatgat ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta
     4681 aagtctcggt aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc
     4741 caacgaaatc cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc
     4801 ctagaatctg tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct
     4861 gccgaccttc tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat
     4921 gaatcagctg aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat
     4981 agcttaccac gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag
     5041 catcatcaat caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt
     5101 ctgtggtagt acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct
     5161 gatacaattc ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt
     5221 gcaccatttt ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca
     5281 gataatagat cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac
     5341 ggcgaaaact ctcagttctc ggtaaaaaac cgcttgtcgg ttctgcgata cgtaaaatgg
     5401 cttctaactg gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag
     5461 tagtactttt gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg
     5521 gacgggagtt tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag
     5581 tagcatccat taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt
     5641 tgttttgcgc tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt
     5701 ttgtcaggta gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt
     5761 gccagccacc aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt
     5821 ttttccattt catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa
     5881 gtactttggg tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtaaatc
     5941 tacgattcgg ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg
     6001 gtcgatcaca cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt
     6061 tagttcaatc agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg
     6121 tccaatcgat agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg
     6181 tctgaatatt gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata
     6241 gtcgtcagca cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag
     6301 catcaaaatc ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc
     6361 catcgttgga agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc
     6421 taatccttcg cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa
     6481 cgtcaagagc gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat
     6541 ctcctttaaa taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat
     6601 tttacaaaag aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca
     6661 gtcaatgatt ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa
     6721 aaaagaagta ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc
     6781 agaagtgatc ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg
     6841 attcgatgtg tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga
     6901 agcgtacaaa aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct
     6961 agtcatcatg aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt
     7021 ttccggaata aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat
     7081 gactgtgctc tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa
     7141 agaccaatcg aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa
     7201 tgaactacat ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat
     7261 acgaagtaag ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg
     7321 attatgaaaa aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa
     7381 atatgactgc atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac
     7441 agcaaattca agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag
     7501 cttaactgaa cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga
     7561 agctgacgta aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa
     7621 cttgcaagta gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt
     7681 gctggaaagt ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat
     7741 cacacttggt tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat
     7801 gatggctggt cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt
     7861 aaagaaaaat gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc
     7921 gatcgaccaa ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac
     7981 tgaagtcagc ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa
     8041 cgactatgta ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga
     8101 gttgaaacaa atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat
     8161 gactggtcaa gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg
     8221 ggtcgtcatc actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc
     8281 agtgacaggc gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt
     8341 attccaccca gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat
     8401 tgaaaaagcg cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg
     8461 ggaaaattca ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg
     8521 cccactggaa gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa
     8581 cgtcaaggta gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc
     8641 agcagaaaga gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg
     8701 ttctggaaac agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa
     8761 aatgatgcag caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg
     8821 ttcaggcgtc aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa
     8881 gaagaaaaag aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg
     8941 tctactgccg gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa
     9001 cgaagaggtg gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata
     9061 tcatgatact gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat
     9121 gcttgatatt aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt
     9181 cgatgaagca tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat
     9241 cagtgaacta atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt
     9301 ccaaaacgcc aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct
     9361 ttggtcgttt tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc
     9421 aacaacaagt cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt
     9481 tatcggaagc acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct
     9541 tgacagttgt ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat
     9601 gcgaaaaatc atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga
     9661 tgatcttcaa tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta
     9721 tgaaacaatc gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt
     9781 ggagaccgaa gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt
     9841 acaattgcca gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca
     9901 aatgacagaa ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt
     9961 ggaacatcaa atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg
    10021 gattccttta ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg
    10081 attcggtgaa tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag
    10141 gcaactatat gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt
    10201 actctgttaa tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta
    10261 atgttccttc aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg
    10321 cttccggttg ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt
    10381 ttgcgccaaa gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat
    10441 cccaaacact gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg
    10501 gcacagaacg gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct
    10561 cacgaaaacg agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa
    10621 atggcagatc atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa
    10681 aagcttctgc tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa
    10741 ttttttttgt catacgattt ttcctccttc aaactatcta taataatctt acaaaagata
    10801 agtaactgga gcaaataaaa acacttactg acaaaagcta catagaagat agattactta
    10861 tttttaacaa aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa
    10921 gctgtaaaga aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat
    10981 aaaacaatgg aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc
    11041 taaaaagagt ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg
    11101 tttcatcgaa actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa
    11161 agaagactta gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa
    11221 catcctttca aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata
    11281 aggtgaccgg atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc
    11341 acttatcctg atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat
    11401 ctaacagtgg ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa
    11461 gcaatccgca caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat
    11521 atcatcgatg ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt
    11581 ttttgttttt tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat
    11641 ccttatagga ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc
    11701 tctattgtta agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag
    11761 cagtatatct gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac
    11821 tgaacaaaca atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga
    11881 acaacagcca aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat
    11941 cgcagcgaca catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct
    12001 cgggttgaat gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata
    12061 tgggtttgct tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa
    12121 tggtcagcaa gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc
    12181 aagttttctt gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga
    12241 aggccaagat cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct
    12301 accagcacct gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc
    12361 cgtagaagta agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat
    12421 gtctgcgttg aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag
    12481 cccatcattt aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat
    12541 aattttggct taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa
    12601 aacagacaaa aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg
    12661 taaacacaca aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag
    12721 aacgttacaa aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat
    12781 taacagtcaa aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc
    12841 cttctatcaa tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact
    12901 ctgtagatct agctgaaaat gaattctatt accatgagat catcggtctg catgtattag
    12961 aagatggaac tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt
    13021 gggtcgttca acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca
    13081 aagaagtcga tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg
    13141 atgaagattg atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc
    13201 atcatcggta aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat
    13261 tattcagata acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg
    13321 ctgctcaaag tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat
    13381 gtcaaaaaaa gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca
    13441 gaagaatttt cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat
    13501 gaacgtatcc gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg
    13561 ggagaattag gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta
    13621 gggaatcaga cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa
    13681 tatactcgcc cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat
    13741 cataaattga ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg
    13801 cctgatcttt tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc
    13861 aagaaagaag aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct
    13921 ttacagtagg ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta
    13981 cgatattccg ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg
    14041 aatccattaa tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc
    14101 cctggtgaca ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag
    14161 ttatttgaag gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta
    14221 cgtaaagttt ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt
    14281 gcaaaaatcg aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt
    14341 gcattacacg gaaaagctgc tcgtatcaaa gaaatcagaa gataaacacg gaaaacttcc
    14401 ttggaaatct gaaatttgtg aaaagtcctt gtatatcaag ggctttttct tttttctcta
    14461 tcacagtttt aagccatttt tggctgtttt ggaccatttt tcaatcatat tcccttaaaa
    14521 attgtcctag attgtccgac attgactatc agtgtcgtca aaatagggca aaatttagta
    14581 gtcagcttga cgacaaatat aatacgaaaa ataccaaaac atttaccgaa tttagcaact
    14641 tttgaaccca agtttctcaa agggtcagct attagcaaaa aagacaaaac atgtactttt
    14701 ctactaaaaa gttgatgtga taggcttcta tctaaataac tattaggagt ttttatcgtg
    14761 aagcaaaatg atggacgtat catttggaaa aatcaaagtg agttaaaact gattttaaca
    14821 attaatgaat tcatcgaaaa acacggaatt acatctagcc gtcaatacca gaaaaagcta
    14881 tcagaaaatc caaattctgc accaagtatg tggttcatta acaaaaaata tggatcatgg
    14941 gagaatttat tgattagtat aggacgagaa aatactgggt atggaaaatg ggctagaatg
    15001 tcagaacaag aattattgga aatagtagaa gcctttatta agtgcgagaa gataacctct
    15061 caacgaatgt acgaacaaaa atcagtaggg aagaatattc cttcacttag taccattaaa
    15121 aagatgttag gagatatacg tccacttttt aaagaaaaaa atgatggatc tcgatttact
    15181 gatttcgaac tgctgttaga actaaaaaat gaaataatcc gattaaaact acaggatgat
    15241 ttatcaatga caaagtttcg aaaactagtt caatccccaa aactaccatc agttgataca
    15301 attatgaaaa gaacgaacaa aaattgggag gaattgatgg cagagattgg atttgattat
    15361 cgaagaatca aaatttataa gcagagaaac aacctatcca agacaaagaa aactaaatag
    15421 tatcactcta aatcagacgt tcaaaattaa aatttggacg tcttttttat tacccaaaaa
    15481 cgaaaggaga tttattttat gaaaaagcca agatttaaga attggcgttt gctcgcaaca
    15541 ctgagtctat tatgccaaac aattggtgga tcgcttggcc caacgattgc ctttgctgat
    15601 gaaattactc atccacaaac ggtaactgtg gaattggatt tggctcacca gtatgctgtc
    15661 gaaggtacat ttagcgatgg tcgtcccatg tcagaagtta ccgttccgca ctatgcagtt
    15721 tacaatggtg tgaagcaaga tattttctgt attgagccag gggtgccgat ttacaatgag
    15781 ttcactcctg gttatgagaa aaatccattg cctgatatgt cggagaaagc gaaattagtt
    15841 tccgttttat ggaaggaagc tggtacggat gtggatacac atattgtggc ccagaagatg
    15901 atttggcaag aagttaatgg ctacacgctt cactcaatta aacgttctga tggtagtgca
    15961 gtaaatatcg cagccattga gtcaaaaatc aatcaagcca ttgcggatta tcaaaagaaa
    16021 ccaagcttcc ataactccac agtgaaaacc gtgttaggtc aatcgactac tgtgacagat
    16081 acgaacggct tgaatttatc ggagtttgat gaagttgtcg agaatatcgc aaatattgat
    16141 tatcgtgtga acggcaatca actagtgatt actccaaatg ccaattcaaa agagagtggt
    16201 gaattaaccc taaagaagtc agctaataca ggaacaccgg tagcttataa aatggctggt
    16261 caacaaactt tgatggcggg ggcaattgat aagcccaata cctacacaat caaaattgat
    16321 gtggaaactg agggttcttt gaagattaaa aaagtcgata aagaaacagg aaatgttgta
    16381 ccgggaaccg ttttccattt agactttgga aaaacattac ctgcaaaaga cgtgaccacc
    16441 gataaagaag gcattgcgac attggatgag attcctcatg gcgcgaaagt aaccattact
    16501 gaaaaatcag tgccagctcc ttatacgatt gacactacac caatcaccgc aaccattaaa
    16561 tctggtgaga ctatttctgt gacttcaaaa aatactcgcg aaaaaggtca aatcattttg
    16621 aataaaatgg gggtggaaac aggaactgat ctttggaacg acaaatactc attagccgga
    16681 aatacgtttg ccattcgcaa agacagtcca accggtgaaa ttgtccaaga aatgaccacg
    16741 gatgaaaaag gtcatgcgga aacaccaaaa gagattgcca atgcgttgga attgggaacc
    16801 tattatgtga ccgaaaccaa agctagtaat ggctttgtga atacctttaa accagtaaaa
    16861 gtcgaattga attatgtcaa tcaaaccgtg gctcttgtta ccagtaacgt aaaagggcaa
    16921 aaccaagaaa ttactggtga aaccactttg acaaaagaag acaaagatac cggtcatgaa
    16981 actcaaggga aagctgagtt taaaagagct gaatacacct tgtttactgt taaggatgga
    17041 aaagcggtca aatggaccga gaactttaaa ccagaattgt tgaagggaac gaaagcttct
    17101 gatgaaaccg taactttgac tttagatgaa aagaatcaag ttgccgttaa acacttagcg
    17161 attaatgagt attactggca agaaaccaaa gcgcctgaag gatatacctt ggatgaaaca
    17221 aagtatcctg tatccatcaa aaaggtggat gatagcgaaa aaaatgctgt gattactcga
    17281 gatgttgcgg caaaagaaca agtgattcgc tttggctttg atttctttaa atttgctgga
    17341 tcagctgctg ggactgccga aactggcttc aatgatctga cctttaaagt atcgccattg
    17401 gaaggaacca gtgagattac aggtgccgaa gatgaagcaa ttacagctta taacgagcaa
    17461 ctaggttttg atggctatgg caagtttgaa aatcttcctt atggggatta tttacttgaa
    17521 gaagtagagg ctccagaagg atttcaaaaa attacgccat tagaaatccg ttctactttt
    17581 aaagaaaata aagcggatta cactaagagt gaatacattt ttacgattac cgaaaaagga
    17641 caaaaacaac caattaaaac ggtgaccgtt ccttacgaga aactgacgaa taaagcattt
    17701 tctgttagct tgaatcgttt gatgctctat gatttacctg aagaagaaga tagcttaact
    17761 tctcttggga cttggaaaga cggaggcaaa gaattgacag accttgattc taccgagcta
    17821 gttgataaat tgagctataa cttgcatgaa atcaaagaag actggtatgt ggtagcgcaa
    17881 gccattgatg tggaagcgac aaaagctgcc caagaaaaag atgaaaaagc caaaccagtg
    17941 gtaattgccg aaacaaccgc aacgttggcg aataaagaga aaacaggaac ttggaaaatt
    18001 ttgcataaat taaccgcaga acaagttttg gataaaacca tcgtcttatt caactatgtg
    18061 tatgaaaaca agaaacccta tgaagcaggt gatgatccag tagcgaaaga tgctagcttg
    18121 aacaatcaag cccaaactgt caattgtacg gtggaacgcc atgtttccat ccaaacaaaa
    18181 gcccacctag aagatggttc gcaaaccttt actcatgggg atgtggtgga tatgttcgat
    18241 gatgtgtcga ttacccatga tgtactggat ggctcaaaag aagcttttga aacaattctg
    18301 tatgcattac ttccggataa tacgaccaaa gaaatttgga aatctggcaa aattgattat
    18361 gaagtgaatg acaaagaatt cactaaaacc gtacttgcgg aaaaagtgga cacaagtaaa
    18421 tatccagaag gtacaagttt taccttcaaa gaaattaact atggcaaaga cggaaacatc
    18481 aatgggaaac acaatgagga tctaaaagaa aaaactcaaa ctttaacacc taaaaaagtt
    18541 ccgacaacac caagtgcacc agaacaaccg gaaataccaa ctgttccaag tgactctcaa
    18601 gaatctagtc ctacagtaaa gacattccca caaacagggg agaaaaattc taatgttctg
    18661 ctgtttattg ggtttacctt gatctttgcg actgctgggt attatttctg gaatcgccga
    18721 aactaaggtg atgtgatgaa acaactaaat aggaaaaacc aacgtgcgcg accgcctcct
    18781 aaaaaaagtg gtcgcgctat ttttgtatcc aaaactaaaa atgaaaagag gaaaagaaga
    18841 tggaattgaa atttgttgtg ccagatatgg ccgaaacctt tggaaagata aggtatgccg
    18901 gcgaaggcga agttttaact gaaggatatg ggcggaatac tacagtaatt ggtcgtagtt
    18961 atcatttgta ttccagtaag caacgagccg atgatattga agtggtggta gctgcggaag
    19021 ccggcgaaaa ggattttgat caagatcaac cactaaaagc cgtgaatccc catttggttg
    19081 ccaaaggcta tgagattgag aatcgtgggt ttaccgacta cgtgttgtat gtcgatgatt
    19141 tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtt gtagattcag
    19201 gactaacgtt tgggaaatta cgattttccg cattacgccg agaagtacgg aagcaaaatg
    19261 aggatggaac ggttagtaac gaggtaaaag aacggactta taatttgaaa tcctctgcgc
    19321 aaggtcgaat gatccaagtt agtatcccag caaatgtacc cttacgcgag tttgcctatg
    19381 acgcagaggt agagttggtc aatccaatca tggatacggt cgccaattat gttttccgag
    19441 aaggaactac tgtcaattgg ttcattaagg ccgatgattt agtcttgaaa cgacaaccag
    19501 atcagggaaa ttctacaaac caaaatgaag gaaagaaata ggtgaagcgt atggaaatta
    19561 atgttgaaca tattttagat tgtctcgatc aatatggaaa aggtgagcta acggaggaac
    19621 aactcactaa agcattaacc tatgatgaaa aaatgtttct gattatgcat caagggttac
    19681 tggaagtagg taacgatgcc gaagaagatt ttgatgtact gaattggtta ggggaagaag
    19741 agtccttctt tatggttatc gaagtaaatg aaagtctatg tcgagaagcc gaaaccgtat
    19801 tggaagaaat tggtgtggaa atgccggatg ccattgaagt ttttttaaag caactagtgg
    19861 aaacgaaaca actccctata gcagtaaatg actagcataa ctgcttgaaa ttggatcaat
    19921 gaaatcctat gaagcttttt tcgattggag gtggaaacca taaaaatgta caaagggcat
    19981 cgtatacggg caggagatca gcacttggtt tatcactttg ttcttggctg gctactcgct
    20041 ctgttcatcg gttggatgag cgtcttttat tttcaagagc ttagacaatt tgatatttct
    20101 aagctatcac tttctactat agaaatcgtt cgttctataa aagatttaat ctatttgcta
    20161 ggaagtctcg ttctttcagg aagcacgatg ttgctttata tacacttttt tcaggatcat
    20221 tggcgcagtt tgtggcatcg acaaaagcta gcccggatga ttctagaaaa tcattggtac
    20281 gaagtaaaac aaactcagag tgaaggattt ttcaaagacc tcaatagtag tcgaaccaaa
    20341 gagacaatca gttacttccc caaaatctat taccgtatga aggatggttt actttctatt
    20401 cgcgttcaaa tttcactggg aaaatatcaa gatcagcttt taaagctgga aaagaagtta
    20461 gaaagtggtc tatattgtga actagtagaa aaggaactca aagactccta tgtggagtac
    20521 actttactgt atgatataat tgccaatcga attgggatag acgaagtagt ggcagaaagt
    20581 ggggctttgc gattgatgaa aaatcaagta tgggcttatg attctttacc tcatatgtta
    20641 atcgctggcg gcacaggtgg tgggaagact tactttttac tcactatcat cgaggcttta
    20701 ttaaaatctg atgccgagct ttttgttctt gatcccaaaa atgcggactt agccgattta
    20761 ggtacggtaa tgcctcatgt ctattcccaa aaggaggaaa tttctgcttg tgtggaagat
    20821 ttctatgagc gcatgatgac tcgtagcaag gcaatgaaag aaatgcccaa ctacaaaaca
    20881 ggagagaatt atgcgtttct tggacttcca ccaaactttt taatctttga tgaatacgtg
    20941 gtttatatgg aaatgttaac gacgaaggaa agtgcggtga ttttgaataa gctcaaacaa
    21001 atcgtgatgt taggtcgtca atctggtttc tttctgattc tagcttgtca aagaccggat
    21061 gcgaaatatt taggagacgg gattcgcgat caatgtgcga catgtttcta actgaaaagg
    21121 ttaggcactt aaaagaagaa aacataagtt tccttttaag gagaactgat attttgagca
    21181 gtggaatgat ggggtaacgc cctgaaacgc tcccttaata ctccgactgg ctgtttaaca
    21241 tatagctgtt agatggtcag aagctcggtg aagtcggctg aaagttaccg taaatatgat
    21301 aaattcatat acgaaagttg ttcagaggtg aatgatgtaa cctataggtc ggaggtctat
    21361 aaaatactca cggtgagaat gtaagtaata ctactgacga acttgcgaat gaaagaatct
    21421 atacacggac tgtatagaaa tgtacaggat tctaccgtat caacggaaca gaatgtatgt
    21481 tgtggtttag taagattatt ggttatgaac aaccatgcaa tgttacaggc attggaaata
    21541 tacaggttca aagcaagcac ctaaaagtat atataaagat aaaaatatcg aaacatggga
    21601 agctggcaat gtggaggaaa ctatattcca ttgaaacatt tgtgacgaag aataataagc
    21661 acatttatgc cagtgaaagt ggtggcacag taccaatgag gatatagaaa tatatcggag
    21721 gaatagccac tagtcaaaag aaatcgttaa ttaaatgatg tatttcatag aatcgagtaa
    21781 gacaaaataa acactacaac ctaagtaaac aaaggtagtg gtgctatgaa taaagtaaaa
    21841 acaaataaaa cacttcgtca tagtgaatac tatggcatga cacaaatatt tgatgaactg
    21901 tatgtaaaaa gtgataaaaa agaagttttt acagagctca tgacaattat tatgagtgat
    21961 gaaaatattc gactagcata cagaaatatc aagaataaca agggcagtta tacagcgagt
    22021 accgataaga aaagtattcg agacattaaa acactgtcag aagatacctt tctaactgag
    22081 gttaaagttc tttttcgctg ttacaaacca cgagcagtta gacgtgtaga aattcccaaa
    22141 ccaaatggaa aaacacgccc tctgggcatt ccatcaatct gggataggtt aatacaacag
    22201 tgtattttac aagtcttaga accgatatgc gaagctaaat ttcacaatta tagttatgga
    22261 tttcgaccaa accgttccac acatcatgcg attgctacct gcaatcgact ggcaaatagg
    22321 gggaagctac actttgtagt agatgttgat attaaaggat tctttgataa tgtcaatcac
    22381 tctaaacttt tgcgtcaaat gtggacgatt ggaatccgtg ataaatggct attaggtatt
    22441 attcgatcca tgctaaatgc tcctatagtc ctaccaaccg gtaaagtcga acaccctaaa
    22501 aaaggtgtac ctcaaggtgg tgtcctatcc cctttactat ctaatatcat attgaatgaa
    22561 ttagattggt gggttgcttc acaatggcaa aataccacga ttgagacaga aaagattatg
    22621 gataggcgag ataaaggcaa aggcattgat aagactaatc agtatcgggc attaagacga
    22681 acaaacttaa aagaaatata cattgttcgg tatgctgacg acttcaaaat cttttgtaga
    22741 aaacgaagtc atgcagataa aatatttcac gcaacgaaaa tgtggcttaa agaacggtta
    22801 tcacttgaaa ttagcgaaga aaaatccaaa attgtcaatc tcaaaaaagg taaatcagaa
    22861 tatctaggat ttgaattaaa gctaatcaag aaaggtaaaa aatacgtagt cgaaagtcat
    22921 atgagcaaaa aatcaatgac aagagttaaa atccaattaa agaaacaatt acgtaaagtt
    22981 gccagaccga agaatcattg cgaacaagca aaagaaattg gattatataa ttctatggtg
    23041 attggaatcc atcaatatta tggaattgca acctgtgtaa atctcgattg cagtaaaata
    23101 gcctatacac taaaaccgtt catcaatcac aaactgccga caaagaaaca tggaaagata
    23161 ttaaatacat ttatcaaatc gaagtatgga aaaagtaaag aaatgcgatg gttgaataat
    23221 gtgccaattg taccgctcaa ctatgttcag tttagacttg ctgtccccct atcagaggga
    23281 acttgcaagt acactgaaag tggacgaagc aagatacaca gtaaattaaa attagaccta
    23341 gcccttttac ttcacatgat gagaaatagt aattatgaaa gaagtattga atttgtggat
    23401 aacagaatat ctaaatattc tgctcaaaag ggaaaatgtg ctatcacggg taaatttctc
    23461 gaatacgaag aaatccattg ccaccatatc gtaccagtaa aacaacaggg tacagacaaa
    23521 tacagcaacc tcattattat tcacaaaaat gttcacgcat tagtccatgc tatcgaagaa
    23581 aaaatcatac acaaatacct gaatgtatta aatctaacca atgaacaaat agaaaaacta
    23641 aatgaattac gtgtcaaagc tggtaatagt gttttaacag tttaacttga aaaatatatc
    23701 atacttaaca ttcttttgat ggaacgccgt atgaagtgaa agtttcatgt acggtgtgaa
    23761 gcgggggaaa atctggagat gacatcaaag gattacctat cgc
//
LOCUS       DAFFFQ010000038        23655 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000038
VERSION     DAFFFQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.038"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            257..532
                     /locus_tag="LQB69_001604"
     CDS             257..532
                     /locus_tag="LQB69_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525772.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LQB69_001605"
     CDS             545..2623
                     /locus_tag="LQB69_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL3525773.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LQB69_001606"
     CDS             2783..4669
                     /locus_tag="LQB69_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3525774.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LQB69_001607"
     CDS             4681..5628
                     /locus_tag="LQB69_001607"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL3525775.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LQB69_001608"
     CDS             5648..6175
                     /locus_tag="LQB69_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3525776.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LQB69_001609"
     CDS             complement(6238..6891)
                     /locus_tag="LQB69_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL3525777.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LQB69_001610"
     CDS             7023..7865
                     /locus_tag="LQB69_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3525778.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LQB69_001611"
     CDS             8023..8919
                     /locus_tag="LQB69_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3525779.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LQB69_001612"
     CDS             8922..9635
                     /locus_tag="LQB69_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL3525780.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LQB69_001613"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LQB69_001613"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3525781.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LQB69_001614"
     CDS             10170..10397
                     /locus_tag="LQB69_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL3525782.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LQB69_001615"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LQB69_001615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL3525783.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="LQB69_001616"
     CDS             complement(11183..11683)
                     /locus_tag="LQB69_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL3525784.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="LQB69_001617"
     CDS             complement(11962..12360)
                     /locus_tag="LQB69_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3525785.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="LQB69_001618"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="LQB69_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL3525786.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="LQB69_001619"
     CDS             13473..14240
                     /locus_tag="LQB69_001619"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL3525787.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="LQB69_001620"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="LQB69_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL3525788.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="LQB69_001621"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="LQB69_001621"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL3525789.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="LQB69_001622"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="LQB69_001622"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL3525790.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="LQB69_001623"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="LQB69_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL3525791.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="LQB69_001624"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="LQB69_001624"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL3525792.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="LQB69_001625"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="LQB69_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL3525793.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="LQB69_001626"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="LQB69_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL3525794.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="LQB69_001627"
     CDS             22691..23566
                     /locus_tag="LQB69_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL3525795.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8191 a         4079 c         4983 g         6402 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagagtatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcg
//
LOCUS       DAFFFQ010000039        23578 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000039
VERSION     DAFFFQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.039"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            44..676
                     /locus_tag="LQB69_001628"
     CDS             44..676
                     /locus_tag="LQB69_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL3525796.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(678..1583)
                     /locus_tag="LQB69_001629"
     CDS             complement(678..1583)
                     /locus_tag="LQB69_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3525797.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(1668..2327)
                     /locus_tag="LQB69_001630"
     CDS             complement(1668..2327)
                     /locus_tag="LQB69_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3525798.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            2643..3587
                     /gene="mvk"
                     /locus_tag="LQB69_001631"
     CDS             2643..3587
                     /gene="mvk"
                     /locus_tag="LQB69_001631"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL3525799.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            3580..4557
                     /gene="mvaD"
                     /locus_tag="LQB69_001632"
     CDS             3580..4557
                     /gene="mvaD"
                     /locus_tag="LQB69_001632"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL3525800.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            4570..5655
                     /locus_tag="LQB69_001633"
     CDS             4570..5655
                     /locus_tag="LQB69_001633"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL3525801.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            5652..6716
                     /locus_tag="LQB69_001634"
     CDS             5652..6716
                     /locus_tag="LQB69_001634"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL3525802.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(6965..7768)
                     /locus_tag="LQB69_001635"
     CDS             complement(6965..7768)
                     /locus_tag="LQB69_001635"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL3525803.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="LQB69_001636"
     CDS             complement(7788..8630)
                     /gene="kduI"
                     /locus_tag="LQB69_001636"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL3525804.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(8646..9653)
                     /locus_tag="LQB69_001637"
     CDS             complement(8646..9653)
                     /locus_tag="LQB69_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL3525805.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(9660..10304)
                     /locus_tag="LQB69_001638"
     CDS             complement(9660..10304)
                     /locus_tag="LQB69_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL3525806.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(10404..11162)
                     /locus_tag="LQB69_001639"
     CDS             complement(10404..11162)
                     /locus_tag="LQB69_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL3525807.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="LQB69_001640"
     CDS             complement(11289..12440)
                     /gene="fucO"
                     /locus_tag="LQB69_001640"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL3525808.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="LQB69_001641"
     CDS             complement(12522..12833)
                     /gene="rhaM"
                     /locus_tag="LQB69_001641"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL3525809.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="LQB69_001642"
     CDS             complement(12835..13680)
                     /gene="rhaD"
                     /locus_tag="LQB69_001642"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL3525810.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="LQB69_001643"
     CDS             complement(13694..14950)
                     /gene="rhaA"
                     /locus_tag="LQB69_001643"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL3525811.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="LQB69_001644"
     CDS             complement(14947..16407)
                     /gene="rhaB"
                     /locus_tag="LQB69_001644"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL3525812.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            16560..17429
                     /locus_tag="LQB69_001645"
     CDS             16560..17429
                     /locus_tag="LQB69_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL3525813.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(17555..18454)
                     /locus_tag="LQB69_001646"
     CDS             complement(17555..18454)
                     /locus_tag="LQB69_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525814.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(18619..19443)
                     /locus_tag="LQB69_001647"
     CDS             complement(18619..19443)
                     /locus_tag="LQB69_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL3525815.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(19454..20749)
                     /locus_tag="LQB69_001648"
     CDS             complement(19454..20749)
                     /locus_tag="LQB69_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL3525816.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(20765..21244)
                     /locus_tag="LQB69_001649"
     CDS             complement(20765..21244)
                     /locus_tag="LQB69_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL3525817.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(21250..22278)
                     /locus_tag="LQB69_001650"
     CDS             complement(21250..22278)
                     /locus_tag="LQB69_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL3525818.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(22295..23401)
                     /locus_tag="LQB69_001651"
     CDS             complement(22295..23401)
                     /locus_tag="LQB69_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL3525819.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
BASE COUNT         6779 a         5199 c         4239 g         7361 t
ORIGIN      
        1 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
       61 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
      121 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
      181 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
      241 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
      301 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
      361 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
      421 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
      481 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
      541 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
      601 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
      661 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
      721 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
      781 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
      841 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
      901 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
      961 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
     1021 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
     1081 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
     1141 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
     1201 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
     1261 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
     1321 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
     1381 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
     1441 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
     1501 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
     1561 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
     1621 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
     1681 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
     1741 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
     1801 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
     1861 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
     1921 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
     1981 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
     2041 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
     2101 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
     2161 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
     2221 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
     2281 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
     2341 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
     2401 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
     2461 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
     2521 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
     2581 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
     2641 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
     2701 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
     2761 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
     2821 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
     2881 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
     2941 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
     3001 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
     3061 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
     3121 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
     3181 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
     3241 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
     3301 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
     3361 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
     3421 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
     3481 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
     3541 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
     3601 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
     3661 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
     3721 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
     3781 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
     3841 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
     3901 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
     3961 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
     4021 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
     4081 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
     4141 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
     4201 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
     4261 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
     4321 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
     4381 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
     4441 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
     4501 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
     4561 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
     4621 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
     4681 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
     4741 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
     4801 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
     4861 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
     4921 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
     4981 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
     5041 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
     5101 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
     5161 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
     5221 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
     5281 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
     5341 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
     5401 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
     5461 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
     5521 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
     5581 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
     5641 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
     5701 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
     5761 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
     5821 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
     5881 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
     5941 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
     6001 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
     6061 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
     6121 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
     6181 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
     6241 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
     6301 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
     6361 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
     6421 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
     6481 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
     6541 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
     6601 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
     6661 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
     6721 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
     6781 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
     6841 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
     6901 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
     6961 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
     7021 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
     7081 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
     7141 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
     7201 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
     7261 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
     7321 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
     7381 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
     7441 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
     7501 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
     7561 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
     7621 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
     7681 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
     7741 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
     7801 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
     7861 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
     7921 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
     7981 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
     8041 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
     8101 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
     8161 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
     8221 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
     8281 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
     8341 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
     8401 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
     8461 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
     8521 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
     8581 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
     8641 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
     8701 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
     8761 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
     8821 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
     8881 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
     8941 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
     9001 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
     9061 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
     9121 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
     9181 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
     9241 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
     9301 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
     9361 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
     9421 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
     9481 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
     9541 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
     9601 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
     9661 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
     9721 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
     9781 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
     9841 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
     9901 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
     9961 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    10021 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    10081 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    10141 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    10201 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    10261 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    10321 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    10381 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    10441 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    10501 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    10561 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    10621 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    10681 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    10741 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    10801 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    10861 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    10921 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    10981 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    11041 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    11101 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    11161 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    11221 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    11281 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    11341 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    11401 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    11461 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    11521 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    11581 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    11641 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    11701 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa
    11761 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    11821 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    11881 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    11941 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    12001 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    12061 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    12121 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    12181 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    12241 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    12301 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    12361 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    12421 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    12481 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    12541 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    12601 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    12661 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    12721 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    12781 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    12841 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    12901 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    12961 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    13021 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    13081 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    13141 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    13201 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    13261 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    13321 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    13381 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    13441 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    13501 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    13561 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    13621 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    13681 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    13741 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    13801 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    13861 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    13921 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    13981 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    14041 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    14101 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    14161 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    14221 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    14281 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    14341 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    14401 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    14461 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    14521 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    14581 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    14641 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    14701 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    14761 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    14821 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    14881 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    14941 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    15001 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    15061 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    15121 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    15181 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    15241 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    15301 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    15361 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    15421 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    15481 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    15541 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    15601 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    15661 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    15721 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    15781 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    15841 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    15901 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    15961 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    16021 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    16081 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    16141 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    16201 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    16261 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa
    16321 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    16381 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    16441 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    16501 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    16561 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    16621 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    16681 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    16741 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    16801 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    16861 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    16921 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    16981 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    17041 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    17101 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    17161 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    17221 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    17281 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    17341 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    17401 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    17461 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    17521 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    17581 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    17641 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    17701 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    17761 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    17821 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    17881 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    17941 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    18001 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    18061 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    18121 agcaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    18181 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    18241 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    18301 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    18361 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    18421 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    18481 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    18541 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    18601 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    18661 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    18721 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    18781 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    18841 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    18901 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    18961 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    19021 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    19081 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    19141 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    19201 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    19261 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc
    19321 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    19381 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    19441 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    19501 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    19561 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    19621 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    19681 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    19741 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    19801 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    19861 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    19921 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    19981 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    20041 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    20101 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    20161 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    20221 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    20281 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    20341 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    20401 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    20461 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    20521 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    20581 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    20641 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    20701 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    20761 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    20821 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    20881 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    20941 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    21001 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    21061 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    21121 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    21181 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    21241 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    21301 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    21361 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    21421 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    21481 ctcatcagtt agttgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    21541 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    21601 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    21661 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    21721 catcacacgg atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccat
    21781 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    21841 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    21901 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    21961 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    22021 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    22081 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    22141 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    22201 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    22261 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    22321 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    22381 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    22441 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    22501 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    22561 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    22621 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    22681 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    22741 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    22801 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    22861 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    22921 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    22981 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    23041 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    23101 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    23161 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    23221 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    23281 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    23341 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    23401 tctttacgcc acttttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    23461 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    23521 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgtttt
//
LOCUS       DAFFFQ010000040        23571 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000040
VERSION     DAFFFQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.040"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="LQB69_001652"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="LQB69_001652"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL3525820.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="LQB69_001653"
     CDS             complement(1172..1867)
                     /locus_tag="LQB69_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL3525821.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="LQB69_001654"
     CDS             complement(1880..2596)
                     /locus_tag="LQB69_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL3525822.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="LQB69_001655"
     CDS             2926..3531
                     /locus_tag="LQB69_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL3525823.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="LQB69_001656"
     CDS             3856..4179
                     /locus_tag="LQB69_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL3525824.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="LQB69_001657"
     CDS             4361..5731
                     /locus_tag="LQB69_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL3525825.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="LQB69_001658"
     CDS             complement(5861..7162)
                     /locus_tag="LQB69_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL3525826.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9350)
                     /locus_tag="LQB69_001659"
     CDS             complement(7392..9350)
                     /locus_tag="LQB69_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL3525827.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="LQB69_001660"
     CDS             complement(9527..9793)
                     /locus_tag="LQB69_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3525828.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LQB69_001661"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="LQB69_001661"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL3525829.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LQB69_001662"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="LQB69_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL3525830.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="LQB69_001663"
     CDS             13137..13700
                     /locus_tag="LQB69_001663"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL3525831.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="LQB69_001664"
     CDS             complement(13953..14474)
                     /locus_tag="LQB69_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL3525832.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="LQB69_001665"
     CDS             complement(14477..15319)
                     /locus_tag="LQB69_001665"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL3525833.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="LQB69_001666"
     CDS             complement(15312..16037)
                     /locus_tag="LQB69_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL3525834.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="LQB69_001667"
     CDS             16213..16509
                     /locus_tag="LQB69_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525835.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="LQB69_001668"
     CDS             17077..18669
                     /locus_tag="LQB69_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3525836.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="LQB69_001669"
     CDS             18682..19422
                     /locus_tag="LQB69_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3525837.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="LQB69_001670"
     CDS             19590..20939
                     /locus_tag="LQB69_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3525838.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="LQB69_001671"
     CDS             complement(21067..21657)
                     /locus_tag="LQB69_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL3525839.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="LQB69_001672"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="LQB69_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL3525840.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DAFFFQ010000041        23568 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000041
VERSION     DAFFFQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.041"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            149..1012
                     /locus_tag="LQB69_001673"
     CDS             149..1012
                     /locus_tag="LQB69_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3525841.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1141..2763)
                     /locus_tag="LQB69_001674"
     CDS             complement(1141..2763)
                     /locus_tag="LQB69_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525842.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3012..3533
                     /locus_tag="LQB69_001675"
     CDS             3012..3533
                     /locus_tag="LQB69_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL3525843.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="LQB69_001676"
     CDS             complement(3719..5449)
                     /gene="spxB"
                     /locus_tag="LQB69_001676"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL3525844.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5614..6432
                     /locus_tag="LQB69_001677"
     CDS             5614..6432
                     /locus_tag="LQB69_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL3525845.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6535..6732)
                     /locus_tag="LQB69_001678"
     CDS             complement(6535..6732)
                     /locus_tag="LQB69_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525846.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6901..7479
                     /locus_tag="LQB69_001679"
     CDS             6901..7479
                     /locus_tag="LQB69_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL3525847.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7574..7711
                     /locus_tag="LQB69_001680"
     CDS             7574..7711
                     /locus_tag="LQB69_001680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525848.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8524..9027)
                     /locus_tag="LQB69_001681"
     CDS             complement(8524..9027)
                     /locus_tag="LQB69_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3525849.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9386..10411
                     /locus_tag="LQB69_001682"
     CDS             9386..10411
                     /locus_tag="LQB69_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3525850.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10758..11636
                     /locus_tag="LQB69_001683"
     CDS             10758..11636
                     /locus_tag="LQB69_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL3525851.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12112..12228
                     /locus_tag="LQB69_001684"
     CDS             12112..12228
                     /locus_tag="LQB69_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL3525852.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13156..13494
                     /locus_tag="LQB69_001685"
     CDS             13156..13494
                     /locus_tag="LQB69_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525853.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13588..13818
                     /locus_tag="LQB69_001686"
     CDS             13588..13818
                     /locus_tag="LQB69_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525854.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14218..14589
                     /locus_tag="LQB69_001687"
     CDS             14218..14589
                     /locus_tag="LQB69_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL3525855.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14863..15555)
                     /locus_tag="LQB69_001688"
     CDS             complement(14863..15555)
                     /locus_tag="LQB69_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3525856.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15558..16343)
                     /locus_tag="LQB69_001689"
     CDS             complement(15558..16343)
                     /locus_tag="LQB69_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3525857.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16580..17978
                     /gene="lacG"
                     /locus_tag="LQB69_001690"
                     /pseudo
     CDS             16580..17978
                     /gene="lacG"
                     /locus_tag="LQB69_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
     gene            18003..18320
                     /locus_tag="LQB69_001691"
     CDS             18003..18320
                     /locus_tag="LQB69_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3525858.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18333..20006
                     /locus_tag="LQB69_001692"
     CDS             18333..20006
                     /locus_tag="LQB69_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL3525859.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20020..21651
                     /locus_tag="LQB69_001693"
     CDS             20020..21651
                     /locus_tag="LQB69_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL3525860.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22523..>23568
                     /locus_tag="LQB69_001694"
     CDS             22523..>23568
                     /locus_tag="LQB69_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HBL3525861.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKE"
BASE COUNT         7357 a         3982 c         4767 g         7462 t
ORIGIN      
        1 ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg
       61 aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt
      121 aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga
      181 tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt
      241 gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa
      301 tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga
      361 atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg
      421 attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga
      481 agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt
      541 attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct
      601 ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga
      661 acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc
      721 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
      781 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      841 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      901 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
      961 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
     1021 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
     1081 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
     1141 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
     1201 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
     1261 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
     1321 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
     1381 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
     1441 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
     1501 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
     1561 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
     1621 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
     1681 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1741 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1801 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1861 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1921 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     1981 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     2041 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     2101 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     2161 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     2221 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     2281 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     2341 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     2401 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     2461 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     2521 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     2581 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     2641 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     2701 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2761 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2821 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2881 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     2941 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     3001 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     3061 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     3121 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     3181 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     3241 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     3301 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     3361 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     3421 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     3481 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     3541 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     3601 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     3661 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3721 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3781 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3841 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3901 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     3961 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     4021 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     4081 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     4141 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     4201 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     4261 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     4321 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     4381 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     4441 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     4501 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     4561 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     4621 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     4681 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4741 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4801 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4861 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4921 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     4981 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     5041 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     5101 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     5161 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     5221 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     5281 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     5341 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     5401 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     5461 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     5521 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     5581 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     5641 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     5701 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5761 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5821 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5881 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     5941 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     6001 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     6061 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     6121 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     6181 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     6241 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     6301 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     6361 cgtcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     6421 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     6481 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     6541 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     6601 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     6661 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6721 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6781 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6841 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6901 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     6961 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     7021 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     7081 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     7141 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     7201 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     7261 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     7321 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     7381 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     7441 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     7501 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     7561 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     7621 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     7681 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7741 atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg
     7801 aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat
     7861 aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga
     7921 taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt
     7981 gtggtttttg gaataaaaaa ataacatatc tcattttacg cttttgttat tttatactta
     8041 aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact
     8101 gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc
     8161 aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata
     8221 aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc
     8281 ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa
     8341 aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc
     8401 ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa
     8461 taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg
     8521 attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa
     8581 taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt
     8641 tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt
     8701 gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag
     8761 gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac
     8821 cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc
     8881 aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg
     8941 tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc
     9001 taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg
     9061 aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg
     9121 aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag
     9181 ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt
     9241 tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt
     9301 tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt
     9361 ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca
     9421 agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg
     9481 ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt
     9541 ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg
     9601 actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt
     9661 tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa
     9721 accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat
     9781 cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc
     9841 ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt
     9901 aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc
     9961 cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac
    10021 cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt
    10081 ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc
    10141 gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct
    10201 agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc
    10261 tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt
    10321 cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta
    10381 tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt
    10441 atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat
    10501 taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct
    10561 atcaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa
    10621 ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa
    10681 agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat
    10741 aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa
    10801 taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc
    10861 aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac
    10921 taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg
    10981 aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt
    11041 atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct tttactagat
    11101 ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt
    11161 ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt
    11221 ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc
    11281 cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt
    11341 tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat acttggttac
    11401 tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg
    11461 attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac
    11521 gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag
    11581 agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt
    11641 agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg
    11701 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
    11761 acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc
    11821 tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag
    11881 ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt
    11941 aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta
    12001 acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg
    12061 atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa
    12121 acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt
    12181 gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac
    12241 ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt
    12301 aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa
    12361 tctttctttt caaattgata ccattcccta attccagtta tatagttaac tacagaacca
    12421 caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct
    12481 cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa
    12541 catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat
    12601 gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc
    12661 agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa
    12721 aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg
    12781 aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa
    12841 taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa
    12901 taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta
    12961 atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga
    13021 tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga
    13081 aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc
    13141 caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt
    13201 gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt
    13261 gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg
    13321 gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa
    13381 aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt
    13441 ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta
    13501 gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg
    13561 atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat
    13621 tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc
    13681 tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact
    13741 gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat
    13801 gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat attatgttat
    13861 tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata aagagtatcc
    13921 agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc tatataaatt
    13981 cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga ttctataata
    14041 tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag aacgggttgg
    14101 tttttaatag atttaataag tactgataat gaaatttctc ctggttggga aaacgaagtt
    14161 ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga taggataatg
    14221 agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc attaaatgaa
    14281 ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat tagtgactat
    14341 attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg catgataaaa
    14401 gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac ttacctagta
    14461 ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat ttcctctttg
    14521 tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga actagtggaa
    14581 atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac tcctgatgat
    14641 ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa ttatagatag
    14701 agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg ctatatttca
    14761 ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta taaaaggagg
    14821 tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat taacggatgt
    14881 ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat ctttatttag
    14941 ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa taattgttcc
    15001 gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa caagacaatc
    15061 agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt gcggtttccc
    15121 agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg ctaaatcgaa
    15181 aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg ttaatggaat
    15241 ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact gaagctcgtt
    15301 ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca atacggcgat
    15361 ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat tcgtccgacc
    15421 atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat catgtatcat
    15481 atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt tgaaatcaaa
    15541 tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc agcaatttgc
    15601 agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg atccgtaatg
    15661 agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt gaatttttta
    15721 ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg tgcttctcct
    15781 tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact gataaatgct
    15841 tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa tgcgccggta
    15901 tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg agcaagaata
    15961 tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa atgcgtcgcc
    16021 aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg aatcaaggag
    16081 gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt ttcagtgtga
    16141 gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac acgcagcagc
    16201 ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga aacatctaat
    16261 tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc tacgatagta
    16321 agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat ctatcatata
    16381 aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt ttttatgttt
    16441 gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt tgtttttgtt
    16501 gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa actgattata
    16561 gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg gcggagccac
    16621 agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg ttgcttggga
    16681 tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt tttatcatca
    16741 atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa gattatccat
    16801 tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag gaatcgcttt
    16861 ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg tgacgttgca
    16921 tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc gagaaacaat
    16981 agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag tcaaggtgtg
    17041 gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag gtgttttccc
    17101 accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata tgatggttgc
    17161 tcgcccgtgt agtgaactat ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc
    17221 actcattaga gacgaaatat gcggcaacag acgcgccgga agataagcat gctgcctttt
    17281 tagacgatgc attgtcgatc cgtttcttat tagatgctac ctatttaggc tattattcaa
    17341 ctgaaacctt gactgctctt gatgagatat gtgaagcaaa tcaggccagc tatcattttc
    17401 ctgaagaaga tttcgttgaa ttgaaaaaag catccacgcg taatgactac ttagggatca
    17461 atcactacca atgccatttt gtcaaagctt acgatggaga aaatgcaatc catcataacg
    17521 gtacgggtga aaaagggacc tctgtttata aagtgaaggg aatcggagaa cgtatttata
    17581 aagaaggaat tcctcgaact gattgggact ggctgattta tccagaaggt ctatatgact
    17641 tgttgctgcg aattaaaagc gattacccac attacaacaa aatctatatc actgagaacg
    17701 ggatggggta taaagatcag tttgaagatg gcattattat ggatcagccg agaatcgact
    17761 atttgagagt ctatttggaa tccttaagca aggcgataac agccggggta aatgtgaagg
    17821 ggtatttttt gtggtcgctt atggatcttt tctcatggac aaatgggtat aacaaaaggt
    17881 atggcttgtt ttatgttgat tttgaaaccc agaagcgtta cccaaaagaa tcagcttact
    17941 ggtacaagtt ggtcagtgaa accaaaacga ttatttaata agtgataatg gaggaagaaa
    18001 agatgaataa agaggaatta caaatgcttg gttttgagat cgttgcatat tcaggcgacg
    18061 ctcgcagtac attattgaaa ttattgaaag aagtaagaca aggaaacttt gaacatgttg
    18121 attcggcgtt gaaagaagcc gacgaaaatt taacccttgc acataattcg caaacaaaaa
    18181 ttttagcgga agaagcatct gggaaagaga tggagatggg attcattttt atccatgggc
    18241 aggatcattt aatgaccact ttattgctga gagatctgat tcaagacttt attgtactgt
    18301 acagacagaa tcaaggatag gagaagaacg ctatggatgg aatcattaaa caaatcgaaa
    18361 aagggaaacc attttttgaa aaaatctcac gtaatagtta tttaggagcc gttcgtgacg
    18421 gatttttaac agcgatgcca gctattttat tttcaagcat tttcattttg attgctgcgg
    18481 ttcctgagat ttttggttac caatggcctg aatctgtctc cacatggcta tggaaagtct
    18541 ataattattc gatgggtgtt gtagggttac tcgtttcagc aaccactgct cgctgcttgg
    18601 ctgattcgat gaatcgtcgc ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc
    18661 tggcctctgt ggtaagtttc ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa
    18721 cggaatatat ggggacaaag ggactactgg cttcctttgt cgctgcattt ttaacagtta
    18781 atttatataa gttttgtgtg ctgaaagata ttaccattaa aatgcctaaa gaagttcctg
    18841 gaacgatttc gcaaaccttc cgagatattt ttcctttctc tttttcagta tttgcggccg
    18901 ttttaattga tttaggcatt cgacaaatct tccaaatctc atttgcagaa gcaatcatta
    18961 cggtgctgca gccgctattt acagcagctg acgggtactt aggaatcgct attatctggg
    19021 gagcgatggc gctgttttgg tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa
    19081 ttgcggcaat tatctatgcc aatgtggaaa ccaatctgca attgttcaat tccggtgaac
    19141 aagcttctaa agtactgacg gtgggtttag ggaattttgt aggaactatg ggaggaactg
    19201 gggcaacatt ggttgttcct tttatcttcc tactctttgc taagtctaag cagttgaagg
    19261 cagttgggaa agcttccttt attccagtaa gtttcgctgt caacgaacca ttgctatttg
    19321 cggcacccat gattttgaac ccttattttt ttattccatt tttgttaaca cctgttgtaa
    19381 acgtttgcct gttcaagttt tttgtcgatg tattgggcat gaacagcttt atgtacgttt
    19441 tgccttgggc aactccagca ccgatcggtt tggttctagg aacgggcatg ggtatcttag
    19501 cttttgttct agcagcggtt ctaatcgtcg ttgacttcct gatctatcta ccattttgca
    19561 aagcatacga tgctgtctta gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg
    19621 aggaaggtgc ttctgccgat gtacgtgagg aagtacctgt ggcagcgagt caagttatca
    19681 gcgatgcaaa taaagaaatc aatgtgttgg tactttgtgc tggcgcaggg acaagtgcga
    19741 tgttagctaa tgctttaaaa aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg
    19801 cgggtgctta tggttctcat tacgaaatgc tgcaggattt tgatatggtg gtcttagcac
    19861 cgcaagtcaa ttcttactat gatgatatca aagaagacac cgatcgctta ggaatcaagc
    19921 tggttgcgac aaaaggagca gaatatatcg gattaacgag agatcctgaa aaagctgttc
    19981 agtttattct gaaccaattt caataaagga ggagccataa tgaaaatggg aatttggttg
    20041 cgtaaagctg ttttatttgc agctgtgtta ggggctttgg gggctgtttc aaaaactgtt
    20101 gaagcatctg ccagcgaatt tattcgcgga gcagatattt cgatcctgtc agatatggaa
    20161 aaatctggtg caaaatatta tgaagacgat atttcaaaag atgccttaga gattttaaaa
    20221 aataatggtg tcaactatgt tcgactgcgt ttgtggcagg atccttataa tagtaatgga
    20281 gaaagttatg gagcagggac aaatgatctg gaaacgacga ttgaccttgc aaaacgagca
    20341 aaaaatctgg gacttaaggt gctgctggat tttcactaca gtgatttttg ggttgatcct
    20401 gggaaacaga atcttcctaa agcatggcaa ggtctgacct ttgaagaaat gaatacggca
    20461 ttgtacgact atacaaagaa cgttctgtcg gaaatgaagc agctggacgt ttatcctgat
    20521 atggttcaga tcggcaatga actgaatagc ggaatgctct ggccttatgg caagtcttgg
    20581 ggagaaggcg ggggagaatt tgatcgattg gctgcttttt tgaagtctgg tatccaagct
    20641 gttcgagaca ctcaacctaa aacaactcca gtaatgctcc acttagctga tggtggcgat
    20701 acaggagcat ttacttggtg gtttgatgaa atcacgagtc gaggtgtgtc atttgattta
    20761 attggagtgt cgtattatcc gtattggcac ggttcaatga atgaacttca aaccaatatg
    20821 aatgcgatca gccaacggta tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat
    20881 acaacagcga atgccgatac tatgccaaat gcttttggtg aagccgaagc agctgcgggg
    20941 ggatatgaac caacaccgac aggacaagca gagtatttgc tggatcttgc cgatcggatt
    21001 caggcagtac cgaataatcg cggggcgggc ttcttctatt gggaacctct ttggtacaac
    21061 ggaaatgtga gttgggcaac acaagcaggt atgaattatc tcagtgttca atccactatg
    21121 ggaaacgaat gggacaacca agcgatgttt gattttgctg gaaacgcctt gccaagcttg
    21181 cgtgcgttta aacaagcagg ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc
    21241 gatggaccaa cgaccagtcc ttccaattgg caaacctggt ttcaacaagg aaccgctaag
    21301 ggtacgatca aaacggaagc gggcactttt tttggggact acaaactaac tttttggcag
    21361 gatcaagcat acgaggcatc agcttatcaa aatgttagtg gcataagcaa aggaacttac
    21421 acattgagtg cctatgtaat gtctggggga gagtttgata ctaatcagtt atatatcaaa
    21481 aattatggtg gagcggaact aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt
    21541 acaatcaaag acattccaat tacaacaggt gtttgtgaaa ttggggttta tagcaaggct
    21601 tcaggaaatg cttggacgaa cgttgatttt gtttcattgg tgaaacaata gactgcggat
    21661 cgtttcgatt caagatcgga ggttcccttc agccacatcg ttagcgtaga aggcaggaat
    21721 cgaacctgca cctcgccttt gccaaaaata accagaaacg atttccagtc atttttacca
    21781 aaacgagtaa agcaccagac ttcatctaca ctttgtatta tgctaaagag agttcaacga
    21841 tttcgtaaga agcaatgaaa aaattgcaaa aagttgtgca gataacataa agtttgttta
    21901 agaaagggta accttagaat tccgtcgtga atatgatttg agcacttaga tggatagaaa
    21961 aaagttctcg gaaaacagtt cgtttttcga gaacttcttt ttatgataag aatgaaacaa
    22021 attaacttat attatagtct cttttggtag ggaaccatct tatttttaat gaaaatcaag
    22081 cattttagct attgaagaat gttgatttat agggattccc cctccatatt atgttggctc
    22141 tttattccca ctctgttttc aacgtaaagt gtataaaaca gaatgcgata ctatcaaggg
    22201 agttaaaaca atatacagaa cagtaaaaca ctataaataa aaataaaagt gcaataaaag
    22261 tgcaattttt gcacctaatc atactctgtt tatatatgaa aactagattc tgaatccaat
    22321 atttggttaa cgagaggcgt tcgaatttga acgtctttta tatattactt tcaaataaat
    22381 atgaggtaga atactatcta ttttcttttt aacccaattg ttataatgga gagtgtaaaa
    22441 tattttgtgt aaatagaaaa aaggaagtcc cttctgtaga atagagttac cacaacacat
    22501 tcacagaaga gaggacttcc ctatgaacga ttttactaca gaaattctaa agactctagc
    22561 gaacaaaggc gatttgaatg aattattccg tgtccatttg gagaaagcag tcaatacgct
    22621 tctcaaaacg gagttaacgg ctttcctaga ttacgaaaag tatgatcgca ttggttttaa
    22681 cacgggtaat tctcgtaacg gctcctatga ccgtacggtc aagaccgagt atggggaact
    22741 tcatctccag attccgcgcg accgcaatgg tgagttcaag caacagactg ttcctgctta
    22801 tagacggacg aatgacacgt tagaggagac cgtcattcac ctcttccgaa aaggtattac
    22861 catgtcggaa atcgcagact tgattgagaa aatgtatggg cattactaca cgccccaaac
    22921 catgtccaat ataacaaaat catttacaga agaggtaacg gcgtttaatg ggcgggagct
    22981 tcatgaccgt tatgctgcta tttatatgga cgcaacgtat attccgttaa agcggaaaac
    23041 cgtcgccaag gaagctattc atatcgcagt tggcattcgc ccggacggat caaaggaagt
    23101 attgagctat gcgattgcac cgactgaatc catcacgatt tgggaggaaa ttttattgga
    23161 ccttcaagag cgcggtttga aaaatgtcct cctgttcatc acggatggct taaaggggat
    23221 ggtaggagcg attagtcggt tctatcccaa agctcgtttt caacattgtt gtgtacacgt
    23281 ttcccgtaat atcagtcata aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt
    23341 taaaatggtg tatcaagcct catctaaaga ggtggcgttg gaagcacgtg gtacttttgc
    23401 ggaaaaatgg aaaaccagct atccaaaagt ggttgaatcg attctttcga acgatcactt
    23461 gctcactttc tatgatttcc ccttggccat acgcaagagt atttattcta cgaacttgat
    23521 tgaatccttt aataaacaaa tcaagaaata cagccaccgc aaggaaca
//
LOCUS       DAFFFQ010000042        23143 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000042
VERSION     DAFFFQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.042"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     misc_feature    111..236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            280..804
                     /gene="infC"
                     /locus_tag="LQB69_001695"
     CDS             280..804
                     /gene="infC"
                     /locus_tag="LQB69_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL3525862.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            876..1076
                     /gene="rpmI"
                     /locus_tag="LQB69_001696"
     CDS             876..1076
                     /gene="rpmI"
                     /locus_tag="LQB69_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL3525863.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            1129..1488
                     /gene="rplT"
                     /locus_tag="LQB69_001697"
     CDS             1129..1488
                     /gene="rplT"
                     /locus_tag="LQB69_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL3525864.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            1740..3047
                     /locus_tag="LQB69_001698"
     CDS             1740..3047
                     /locus_tag="LQB69_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL3525865.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAIFA"
     gene            3124..5253
                     /locus_tag="LQB69_001699"
     CDS             3124..5253
                     /locus_tag="LQB69_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL3525866.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKQQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            5228..5917
                     /locus_tag="LQB69_001700"
     CDS             5228..5917
                     /locus_tag="LQB69_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525867.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(6325..7011)
                     /locus_tag="LQB69_001701"
     CDS             complement(6325..7011)
                     /locus_tag="LQB69_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3525868.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7147..7341)
                     /locus_tag="LQB69_001702"
     CDS             complement(7147..7341)
                     /locus_tag="LQB69_001702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525869.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(7391..7831)
                     /locus_tag="LQB69_001703"
     CDS             complement(7391..7831)
                     /locus_tag="LQB69_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL3525870.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(7835..8680)
                     /locus_tag="LQB69_001704"
     CDS             complement(7835..8680)
                     /locus_tag="LQB69_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBL3525871.1"
                     /translation="MLINEQLVKRIITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            8829..10247
                     /gene="pepV"
                     /locus_tag="LQB69_001705"
     CDS             8829..10247
                     /gene="pepV"
                     /locus_tag="LQB69_001705"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL3525872.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(10304..11251)
                     /locus_tag="LQB69_001706"
     CDS             complement(10304..11251)
                     /locus_tag="LQB69_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3525873.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(11474..11824)
                     /locus_tag="LQB69_001707"
     CDS             complement(11474..11824)
                     /locus_tag="LQB69_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL3525874.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            12024..13103
                     /gene="pepA"
                     /locus_tag="LQB69_001708"
     CDS             12024..13103
                     /gene="pepA"
                     /locus_tag="LQB69_001708"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL3525875.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            13247..13567
                     /locus_tag="LQB69_001709"
     CDS             13247..13567
                     /locus_tag="LQB69_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL3525876.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            13589..14053
                     /locus_tag="LQB69_001710"
     CDS             13589..14053
                     /locus_tag="LQB69_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3525877.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            14258..14863
                     /locus_tag="LQB69_001711"
     CDS             14258..14863
                     /locus_tag="LQB69_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL3525878.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(14902..16191)
                     /locus_tag="LQB69_001712"
     CDS             complement(14902..16191)
                     /locus_tag="LQB69_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL3525879.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENIKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            16619..17098
                     /gene="rlmH"
                     /locus_tag="LQB69_001713"
     CDS             16619..17098
                     /gene="rlmH"
                     /locus_tag="LQB69_001713"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL3525880.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            17183..17692
                     /locus_tag="LQB69_001714"
     CDS             17183..17692
                     /locus_tag="LQB69_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL3525881.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            17792..18442
                     /locus_tag="LQB69_001715"
     CDS             17792..18442
                     /locus_tag="LQB69_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525882.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            18893..19831
                     /locus_tag="LQB69_001716"
     CDS             18893..19831
                     /locus_tag="LQB69_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL3525883.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            19844..20896
                     /locus_tag="LQB69_001717"
     CDS             19844..20896
                     /locus_tag="LQB69_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3525884.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            21162..21797
                     /locus_tag="LQB69_001718"
     CDS             21162..21797
                     /locus_tag="LQB69_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL3525885.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYRLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            21988..22779
                     /gene="msrA"
                     /locus_tag="LQB69_001719"
     CDS             21988..22779
                     /gene="msrA"
                     /locus_tag="LQB69_001719"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3525886.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         7376 a         4068 c         4789 g         6910 t
ORIGIN      
        1 ggaatcactg ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta
       61 tgaaaaacat tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc
      121 agaagcaccc gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt
      181 tctattaggt gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat
      241 tcggtaagct tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg
      301 atggttaacg acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa
      361 ttaggagtta agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt
      421 gtgttagttg cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac
      481 cgtttcgaac aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta
      541 aaagaagttc gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat
      601 gcacgtaagt tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt
      661 gccattaccc ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg
      721 gatattgcaa cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg
      781 gcaccgaaaa acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt
      841 cggataacga aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg
      901 gatcagcaaa acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta
      961 caagtcaccg tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca
     1021 tggtttctag tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag
     1081 tacaaataac agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt
     1141 aaaggtggaa cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac
     1201 tacggttcaa aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac
     1261 tacgcatatc gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt
     1321 atcaacgcag cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa
     1381 gttgctgaaa tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca
     1441 gcatttactg cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta
     1501 taaagaggaa agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct
     1561 ccatcaaatg aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta
     1621 attgaaaact gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa
     1681 tgagtttgac tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca
     1741 tgagtgatat tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact
     1801 gtgtaaaagg ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat
     1861 tagcgctagt cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg
     1921 atgtgttgac gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa
     1981 atgtcttctt tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc
     2041 tgattcgaaa ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat
     2101 gtattgtaac cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg
     2161 cagttattgt gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct
     2221 catttatggg ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc
     2281 aaacgattta tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc
     2341 aaattggtat gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat
     2401 caatgaacaa aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg
     2461 caccaatgat ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc
     2521 ttccaatcat cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat
     2581 tgcgcggagg ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag
     2641 tagatgttgc accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg
     2701 cgtatgcttc tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag
     2761 tcttggcaat cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc
     2821 tagtcggttc aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc
     2881 cagccttttt gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta
     2941 cacaatcttg ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga
     3001 agaaatccat cccaactggt tggatcatcg gagcgatctt tgcctgatca cgttcttatt
     3061 gtacgggaac gcctagtaaa caaaacgtcg ataaaaacga ttagatgaat ggaggaaaag
     3121 aaaatgaaac gttttgtgcg aatgggaatt gatgttggcg gtacacatac gaaagcagtc
     3181 gcaatcgata atgcgacgca cgaaatcatc ggaaaatctt cagtgaaaac aacccacgat
     3241 gatgtccgtg gagtagccgc aggtgtcgtt cagtcttttc aaaattgttt gcgggaaaat
     3301 aatatcagtc cagaagatgt tgtgtttgtt gcacattcta ccacacaagc gactaatgca
     3361 ctgattgaag gagacgtagc aaaagtcggc gtaatcggga tggcaaaagg tggattagaa
     3421 ggttttttag caaaacaaca aactagattg aacgatatcg atttagggaa taagaaaaaa
     3481 atagagattg tcaatgcatt cttacctgtc aaacacttaa atgtagaccg tgtttcagaa
     3541 acgatttctt ctttagaaag agaaagagcg gaggtcttgg tttcttctat ggcatttgga
     3601 gtagataatg gggaaccaga acgagttgtt tatgaagcag catcggtaaa atcgatccct
     3661 acaacgatgg cttctgacat cacaaaatta tatggattga caagaagaac tcggacagca
     3721 gcaatcaatg catctatttt accaaaaatg ctagatacgg caacctctac agaagattcc
     3781 gtacgagaag caggggtcaa tgtttctttg atgattatgc gcggagacgg cggagtaatg
     3841 gaaatcaatg aaatgaaaaa acgacctgtt ttaaccatgc tttccggtcc agcagcatca
     3901 gtcatgggct ctttgatgta cttgagagcg tctaatggtg tttattttga agtaggcgga
     3961 acaacaacca atatcggggt cattaaaaat ggacgtccag caatcgatta ttcaattgta
     4021 ggtggccacc caacatatat ctcttcatta gatgttcggg ttctaggtgt ggcaggcggt
     4081 tcgatggtac gggctaatca gtcaggaatc atcgatgtcg gtccacgttc tgcacatatt
     4141 gccggtctag attatgcagt atttacggag acagaaaaaa tcaaaggtcc taaggttgaa
     4201 tttttctctc ctaaagaagg agaccctgct gattacgtca aggtagtgat ggaagacgga
     4261 gaagaagtga ctattacaaa tacatgtgca gccaatgtcc ttggattagt gcaagaagaa
     4321 catttttctt atggaaatgt cccatctgca cgaaaagcca tccaagcatt agctgattat
     4381 tgtcatacga cggttgaaga catcgcagaa cagatcatgg aaaaatccta tgctaagatt
     4441 gaaccggtga ttttagaatt ggcagacaag tatcacttgg agaaagatca gatttctctt
     4501 gtaggtgtag ggggcggcgc tgcctcattg attacttact ttagtaataa aatgggtgtg
     4561 aaatattcga ttcctgaaaa cgccgaagtg atttcttcta ttggtgtagc acttgcaatg
     4621 gtccgagatg ttgtagaacg tatcatccct tcgccaagta aagaagatat acgatcgttg
     4681 aaaaatgaag cgatgaataa agcgattgaa tcaggcgcta caccagaatc gatcgaagtc
     4741 cacgtagaaa tcgatccaca gacttcaaaa gtaacggcta tcgctactgg ttctacggaa
     4801 gtgaaagcaa ccgatctaac caaggagatc acaacagaag aagcactaga gcttgctgca
     4861 gaggatatgc gattaaataa aaatgaagtt tgcttattgg aaaatacacc tttcttttat
     4921 gtatgcggcg aacagaatcg ttcaaaaaat gctggaagtc ttcggatcat cgatcaaaaa
     4981 ggatttatca aggtccaacg tggacatgct tcatgtatga aaacaactgc agccaattat
     5041 atgacagctg tcgaacaact ttgggaagat atggcagttt atcaaactga attgattgcc
     5101 cgaccagaat tttatctctg cttaggagca agagtctctg attttacagc gacggatctg
     5161 gaacaactac agcttttgat ggatctggaa gtatctacta tggaaccgga ggaagaagtg
     5221 atcgtcgttg ccggaaatat caaacaaaca taagttagaa gagttactaa acgaattaca
     5281 acagatcccc gaagaaattt ggggattcta ccagtttcgg cgagatttat tttggaaaaa
     5341 gattcctttg tccaagcaga aaattcttat acaacaatca atcgattgtg gtatcgaaac
     5401 ggcatgtagc atcaaaaaaa agtacccttt tgccgatgta ggagaaattt gtgaacaaat
     5461 ggctgttcca atcgtttcct gtgaatcaga gcaaataaac gaacgaatca cttttgcaac
     5521 ttatgcagaa gatgaaggga ttcgattgat gacggagccg ttagaaaaat taaaatgttc
     5581 aggactagct tcgatctcta aagaaacagc gcaagctttg attattggac atgaactatt
     5641 tcaccacatc gaagcttctg ttaagggaat ttatacgcaa agcgaaaaaa ttgtgctgtg
     5701 gcggttgcct ttttatacac atcaatcaaa cattcgtgca ttgagcgaga tagcagcgat
     5761 gagtttttca aaagagatga atcaaagcga tttttcgcct tatgtattag aggcagtctt
     5821 actttggcca tataatgaaa ttcgtagcca agggatctta gaagaaataa aaaaaataga
     5881 aaaaagatgt acggagtatg atttagatta caaataaaga gactgtgaca aaagtcgtgt
     5941 cacagtcctt tttttccgaa taaacgatgt tctcctgtca actcctcggt cacaagtcaa
     6001 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagatgaa
     6061 cggcaggctc acgacctctt ggaaaacggt gttcaaaagc ttgcatctgc ggtaataagt
     6121 ccagccaagt taaaaacata agaagctatt tttacttggc tgttcttatt acttgatgca
     6181 gggcagcttt ttcacaacct ctcaataaac ggtgtgcaat cagctgaccc tcggtattag
     6241 ctcaaaaaaa acggcaaaat atgaggaact attttgccgt ttctttctct aatactcagg
     6301 tcaaaacgct gattccaaaa cctcttattt gatgggtctg tcctgaatat cttcaaaata
     6361 atcggggtaa ttatgaatca cgtattcttg ctcctcgata atcatatgta aatgcatcgt
     6421 gcaaagaaat ccgacttcat caaagttatg atcaatcatt gattgaagca ttgtctgatg
     6481 ttcatctaat actcgacccc agtctaattc tgaaatcgtc aaacgtaacc atctgaaacg
     6541 gttcaagtgc gtactataac tttctagcca atcccaaatt tctcgttttc cagcaatttc
     6601 ataacaagtt cggtgaaaat cagtatccaa atcaaaaaaa gcttttttat tttgagcgat
     6661 tactgcctga tcggtttgtt ctagtatttt ttctagttta gcttgatctt gcttggtcat
     6721 tttcgcactg cattcttgca ggatcggttt ttccaacttt tcccgcgtat aacgggctaa
     6781 tgaagcagat cggatatcga tttttgaaac ataagtgccg gactgtggaa tggtttttac
     6841 aagctcttga ttgcgcagtt ggatcagtgc ttctcgaatc ggtgtacgcc cgatagctag
     6901 ttgttcttct aacactttat cggagatttt acttccaggt gctaactcgg aataaatgat
     6961 ttgtttacgt atagattcat aagcttgctg ttgcaaatta ttgataacca tttaaccttc
     7021 ctttctatgc agatataatc tattataaca taaatcgctt acaaatcgtg tgggcataaa
     7081 aacacagtct gcaaaaaaag ccctgatagg catgcctatt agagcctcga aaagaaaaat
     7141 attgaatcaa tctggtcgta cctcttgtat cggtttgaca cgaccgccga ttcttttagc
     7201 aaaagcttcg gattcttttt tgtctttgaa aatgagcaga ttgtctaagt ttttttcccg
     7261 agtgcgacag ccagttttat cgatgtaacc gtgcattact ttaacgacat acatggggat
     7321 cacctgcttt tttcttttca ttatacgaac tactcgacac aatacaagaa aaatgcttag
     7381 acggtcttta ttaagaattt acatcatcca aagaagcgta cgcttggaat aactgatcta
     7441 actccggatt gactttgctc agcttcacta atcgttgcga atcagaaagt aaaaaacagt
     7501 gtttactact gccagtagtt tttaattttt tgtcttcagt tcccacaatt tcgtagctaa
     7561 aatccaaccg tgtccccgta tacttttgga tagttggatg gatgctcact ttttctccgt
     7621 aatggatcat ttctttatag ctgcaattta cttccagaac ggggatgatg atcccagatt
     7681 cttcgatttt tttataggga aaccctagat gtaccaataa ggctacccga gcctcttcaa
     7741 accagcggat ataattgctg tgatgaataa tccccatttg atctgtttca taataaaaag
     7801 gttctcgcac ataacctgta aatttctcca tgactcatgc ctcgttttcc attgattttt
     7861 tacaaaaata attcatccat ttcggaagtg cttcgctgat ttgtgtcggc aaaacaatat
     7921 attgttcttt tgccagctgg tctccaatga aactatgaag ataaatagct gctgcaatcg
     7981 tttggtcatt ttgaggaaac tgggctaaaa atcctgtgat catcccagct aaagtgtcac
     8041 ccatgccgcc cgttgccata gcagcagaac ctaaaggatt ggtgtagaag tgctggtgac
     8101 cataaatttt tgtctcatga cttttcaaaa cgagcacagc accgatttcc tgttgcttct
     8161 ttttgttttc tttatcccgt tgttcttgta tcggtagaga actcaaccgt tgccactcca
     8221 tttgatgagg tgtaagtaca gtttgttcgg gatagggtag caaaagatga ttagcagcaa
     8281 gtaaacttaa agcagagcca tcaatcacca gccattgttc ttttgattgg ttttctagta
     8341 cttgtttgaa acgttccaaa ctcttagaag aagtacccaa tccgggacca atcaagagta
     8401 catctgcttg ctttattacg gaaagaaact ggtcagtatc cgtccaaact acaatcattg
     8461 cttctggcag atgactgtga agagcactcc gatttttttc atcagttact acagtcgtga
     8521 gacccgctcc gctgtgtacg gcagctaaag cactcataat gattgctccg ccatattgtt
     8581 cattgccgcc aatcaaaacg atacgtccaa atgttccttt atgtgaaatt tttggacgag
     8641 gtgtgatgat tcttttcact agttgttcgt ttatcagcat ctaaaaacct ccttttactc
     8701 attatagcgt atattttttt tagagaaaaa cagataagaa aaggaattgt cagatttttt
     8761 gaactatttt tttctgtaat ttgtggtatc atgaacacga agacttaaat aagtggagga
     8821 ttaactttat gacaattgat tggcaaaaag aagtagaagc acgtaaagaa gacttgcttg
     8881 aagacttgaa aaatctttta cgtgtcaaca gcgagcgcga tgactcaaaa gtaacaccag
     8941 atgcaccatt tggacctgga cctcgcgacg cattgaaaca tatgttagct tatggcgaac
     9001 gtgatggatt taaagtaaaa aatgtcgata actatgcagg gcatattgat cttggcgaag
     9061 gagacgaaac gctagggata tttggacaca tggatgtcgt tcctgcagga gatggctggg
     9121 acacagatcc ttatgaacca gtaatcaagg atggaaagat ctttgcacgc ggatcaagcg
     9181 atgacaaagg tccaagtatg gcagcttact atgcgatgaa gatcattaaa gaacttgact
     9241 tgaaactatc aaaaaaagtt cgcttcgtag tcggcagcga tgaagaaagc ggctggggcg
     9301 atatggctta ttatttcgaa catgaagaag aaccagattt tggtttctca ccagatgctg
     9361 aattcccaat catcaatggt gaaaaaggaa atgtttcttt agctttacgc ttcaaaggcg
     9421 acaatgcagg agattatgtc ttgaaatctt ttgtttccgg acttcgcgaa aatatggtgc
     9481 caggaacagc aactgcagca cttcaagtac caagtgcaga tgcagctatc gcgatggaag
     9541 aagcatttta ccaattcatc gaagcaaatc cagtaagcgg aacgattgaa gcggacaata
     9601 cgtatgtaaa aatcgaatta gttggtaaag gcgcacatgg tgcaagccca caatctggta
     9661 tcaatgcagg ttctttcttg gcattgttct tagataacta tgaattccta ggatctgcta
     9721 aacaatttat ccatgtagca gctgcttatg tccatgaaga tttttatgga gaaaaactag
     9781 gagtcgctta tgaagatgaa aaaatgggca aattgacaat gaatgctgga ctatttgctt
     9841 ttgaagaaaa tggtacagag gaagcgaact tcatcaatat gaatttccgt tttccaaaag
     9901 gtgtaacagt agacggattg caaagtgaca tcgaacaaac agtggggcaa gaaggcgcaa
     9961 cagttactcg aggtgctcgc gtaatggaac cacattacgt accaatggat gatccattgg
    10021 tggctacatt attgcaagtc tatgaagacc acactggaga aaaaggctac gaacaaatca
    10081 tcggtggcgg aacttacgga cgtttgttga aacgtggcgt tgcttatgga gcgatgttcc
    10141 caggatatac tgatacaatg catcaagcaa acgaatttat gagtcttgat gacttgttca
    10201 gagcaacagc aatctatgca gatgccatct atcgtttggc gaaataatta attaaaaaga
    10261 ggatcaagaa ttgcaaatta tttttgcagt cttgatcctt tttttacttt ctactgtggt
    10321 attcatgata aacatgatat agattcaatt catattcttt cttagcgttc gtgacgactg
    10381 tatccaaaat caaccctgtt actaatgaaa gaagtgctaa agtcatcaat ccagtactta
    10441 aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat atattcgcga atcacaggaa
    10501 tccccgtgac caatccaaat aaaaagaaga agatggtcca aatcccaaag aaaagcaaag
    10561 gcttataatc tttaaacatt ttaataatca tcatgatcac tttgaatccg tcagagaatg
    10621 tattcaattt tgattcgctg ccttcaggac gatcacgata atcgattggg atttcaacga
    10681 acttgaattt tttatctaaa gcatggatcg tcaattccgt ttcgatttga aaaccagaac
    10741 tcataatagg aaaacttttg acaaagatac gattgaatcc tcgatagccg gtcatgacat
    10801 caagaatttt tgttttatac attctagtaa ttgtatttct aacaaggtta tttccaaaat
    10861 cgtggaaagg tctcttattc tcatcaaagt aggttccatt tgataggcga tcaccaatga
    10921 ccatatctgc ttcaccagaa cgtaatttct cgagcaaacc atgtacagct tctgcaggat
    10981 aagtatcatc gccatctacc attaaataat agtcggcatc gatatcaaag aacatttggc
    11041 ggatcacatt tccttttcct tgacgaggtt ctttttttac gattgctcca ccttgaacgg
    11101 ccaattcgta tgttcgatcg gtcgaattat tatcatagac ataaatatct gcttctggta
    11161 attctcgttt aaagtctgca atgactgttg aaattgttgc ttcctcattg taacaaggaa
    11221 tcagaacagc aattttttct tgttgttcca tttttcactc tccttaacta tagaaaacaa
    11281 aagctaaatt aaaaatacct cataatcaaa ggaaatgcta gtttttcccg ctaaatatca
    11341 tttatttaaa aagaaaatac tggaatatat gtttggaaag ctctatctaa aaaaccaccc
    11401 actacaaaag aaggacatga tacctctctt ttgaatagtg ggtgcgtatt cgttctctca
    11461 cttttttgac gtattaatct gttgcacctt tttttcttga aatttctaat acggttcctg
    11521 tatgagcatc tgctaaaaat tcataataaa cagtttcgcc atcttctatt cgagaaagac
    11581 ctccccgata agcactcgac cgaacggcga atttcctaat aggttccttt tcaaaagaga
    11641 tccaagaacc ttcgattgca ccttctttta gaaaagatga cttgatattt tcaagtactt
    11701 ggtctgctga caatactttt ttgcgatgat accaaatcgt tgaagctacc ccgctcaata
    11761 atcctagacc gattccaact gctaacccgc ctttaaaata gcttagttcg ctttcttcat
    11821 acatgtggac atacctcctt cttctcactt gttactcctt gtgcttattt taccacactc
    11881 tcatattttc tttgaaataa ataaccgcta ccagaaaaaa taagctataa taaatacgat
    11941 cggtctgcag aaaaaatcat attttatccg cagatcaata taagtctgag aggagtttag
    12001 tctgaaattt agacgaatag catatggaag aaaaaacatt tcaacgaatc aaagagttaa
    12061 cagaattaca aggaacaagc ggttttgaac aagatgttcg cgcttatatg gaaagtcata
    12121 tggaaccgct ggtagatgag ttacagttag atggtttggg cggcattttt ggtctgcgcc
    12181 atcatgaaga agcggatgca ccccgtgtaa tggtcgcagc acatatggat gaagtcggat
    12241 tcatgctgac acaaatccaa gaaaacgggc tttttaaagt cgtacctcta ggcggctgga
    12301 atccttacgt tgtttctgct cagcgtttta cacttaaaac ctctactggt aagaattatc
    12361 catgtatttc ttcttccgtt cccccacatt tattgcgagg aacaagcgga caaaaatcag
    12421 tagaagtaac cgatattttg ttcgatgctg ggtttgaatc aagagaagag gcaatgagtt
    12481 acggtgtcct tccaggagac acaatcgttc cttacgctga aacgatcaaa acagccaacg
    12541 gaaagaatat tatcagtaaa tcatgggaca accgctatgg ctgtacaatg gtattagaag
    12601 ctttagaagc gttgcaaaat gaaacattag gtcatacatt gattgcaggc gcgaatgtcc
    12661 aggaagaagt aggcttgcgt ggttctaaag cttctgtcaa taaattcaag ccagatctgt
    12721 tctttgcagt agactgctct gcagcagatg atacagtgac gaaaaatggc acattcggac
    12781 atttaggaga aggtacattg atgcgtatcc aagatccagg tttgatcatg cttcctcgtt
    12841 tgcgcgagta cttgctggat atcgctgaaa caaacaatat cccttatcaa tactttgttt
    12901 caaaaggcgg tacagatgca ggtgctgctc atacacaaaa cgaaggaatc ccaagtacag
    12961 tgattggcgt agtgggacgt tatatccata cgcatcaaac gatgttcagt attcgcgatt
    13021 ttgaagctgc acgagaaatg ttgattcaaa cactgaaagg tttggataaa tcaacagtga
    13081 atacgatcgt atacggaaaa taatgcgata gtgaaactat aaaatctggc aaaagattgc
    13141 tttttgccag atttttttta taaaaagagg atcaatgttt gttagttcga aagaaaggtg
    13201 tataatttaa ttgaaaaata cataaacaga aagtagagga caaagaatga ttatcccaaa
    13261 tagtttagaa gaattagcag gctacgtaga aactggaaag agtgtatttt tctttactgc
    13321 cggctggtgc ggagactgcc gttttatcaa accgcaaatg cctgaaattg aaaatgactt
    13381 ttctgattgg caatttattg aagtagaccg tgacaagtac atagatgtag ccgctgaatg
    13441 gaacattttt ggtatcccaa gttttgtcgt gatccaagac ggaaaagaat taggtcgtct
    13501 agtgaataaa gatcgtaaaa caaaacagga aatcgaatcg ttcctagaaa gtgtaacaaa
    13561 aggctgacat ctgttaggag gggtcaagat gaatcaagaa tataagaata ttttagtagg
    13621 tatcgacggt agtgaacaag cacttgctgc tttcaaaaaa gcggtagaag tggctcgccg
    13681 taataatggg aaagtatatg tagcaaacgt aatcgatcag caattttata actttatggg
    13741 ctatgcacct attgatcaaa atctagtcga tcagcaaact gaaacagcca aggaaatgat
    13801 cgaagaatgt aaagaatatg ggaaatctgt tgattacaca aatatagaag gaattattgc
    13861 atatggttct gttcgtgaat ccatggcagt caaattgcca aaaaaatatg agatcgatct
    13921 aatcatggtt gggcagtcag gtctaaatgc agtcgaacgc tttatgacag gaagtgtagc
    13981 cagctacgtg atacgtcatt caccttgtga tgtcttgatc gtttctgacg aagaagaaca
    14041 gccaagtaat taagcaagtg gaacttgtac gaccttttta atcaattaac taaaacagta
    14101 aagggactgt gacaaaagtc ttgtcacagt cccttgttac gaataaatgg tgttccagaa
    14161 gaggcttctt cggaaataaa gcatgaaaaa aatttcatgc agtgaatgca attttttgtt
    14221 aagataaatc tcagcaaagg ataatggagg aaagaacatg atttttgctt ataacaaagc
    14281 acacgtcgga gacaccttga tggttattat agccgacgat aaaggaacag aaaacactgt
    14341 tgaacgcaaa tggaatgttg ctcggatcaa agatgaacaa ggtcaagttg ttgcttggaa
    14401 ctttttccat atctccgatc atctaacaat cgaagggaac ggccaagtaa gcttaacaga
    14461 tgaggaatta gcgaaattga atcgattgat caaagaagca ggattcgaag aacagcttga
    14521 agctgatcat gaaccgaaga tcgttgtcgg gtacgtaaaa acatgcgtcc cacatcctga
    14581 ttctgaccat ttatcgatta ctgagacaga agtagacaat ggtacagtat tgcaaatcgt
    14641 ttgcggtgca cccaatatcg aagctggaca aaaagttgtc gttgcaaaac caggagctat
    14701 gatgccagat ggaatgatga tctggccagg aactttgcga ggggtagata gctatggaat
    14761 gatctgttcc gctcgtgaat tgcatttgcc aaatgctcca gcaaaaaaag gaatcttagt
    14821 attgcctgag gatgccgtag tcggagaagc atttccaaaa taaagaggtt gtgacaagtc
    14881 tttgtcacaa cctctttttt attaattttc actattttga ttttgtttta atgccgattg
    14941 atcgatcgtc aattcaacag tagttgtttt ttctttcgag ccttcatagt aagtgatttt
    15001 gacagaatct cccacttttt tcttatacaa agcagattga agttctacac cagaagaaac
    15061 ttctttgtca tcgatcttag taatgacgtc gtattgtttc aatccagctt tttcagccgg
    15121 tgtagctgtt tggacatttg tcacgatgac accgttagtt acagatgaag gaatcttcaa
    15181 gatttgttct tgttgctgag tagaaacggc agataagtcg accattgtga tacctagagc
    15241 tggacgtgtc acttttccgt ctttttccag ttgattgatg atattcacta catcgttact
    15301 tggaatagca aatcccattc cttctacgct tacattagaa gtagattcag aagtacttgc
    15361 gatcttactt gagttgatac cgataacttg tccttcgata ttaactaaag gaccaccaga
    15421 atttccaggg ttgattgctg catctgtttg gatcgcattg atattgactg tttcgttcga
    15481 ttcattcgta cttgtcactt gtcggttcaa ggaagaaatg attcctgaag tgacagaatt
    15541 tgcatattca gatcctagtg gggaaccaat cgcaatcgca ggctcaccaa ccttcagagc
    15601 acttgagtca ccaaaagagg caactgtttc gactttgtct gaattgatct tcaacaccgc
    15661 taaatctgaa taagcatctg ttccaactaa ttcagctttt accttcgtac cgtctttcat
    15721 caagacttca agaccttgct ggccatctac tacgtgatta ttcgtgacta cataagcagt
    15781 attcccgtct tttttgtaga tcacgccgct tccttcacta taagcttcca aattactatc
    15841 atctgagctg ctactttctt cttgctgccc gaatagtccg ccaaaaccgc ttgattggtt
    15901 ttgactttgt aagttgataa cagaaacgac agcgtcttga actttatcta ccgctttggt
    15961 gatatcagaa tctacattga ctttgatatt ttccacaact gtttcaccgg cagaattctg
    16021 attgccgctt gtagcagtag aactattgtt gccagtgccc atcgctgcat aaaaaatgcc
    16081 agctgtcagt agtcctccaa cgatccctcc aacaagcccc agacctaact tacgccagag
    16141 gctattattt ttactttttt tcatctttgg tgtcacattt tttctatcca tgtgttcctc
    16201 ctccaattac attcatttat ctactgttag tatagtctta tttgttgact ttagtctact
    16261 ttgagactgt gaacttttta tgaaaaactt taggaaaaat cattgttttc agatgaaaat
    16321 atttgatttt ctttttccac agatactgtg taaaactacg cggataggat tggaaaaata
    16381 agggataaaa catatccaca gcctgtgtat aaagtggata aattcttata aaacgtatgt
    16441 ttgcctaatg aatgtgatga aataaggtaa aataacactg ttttctgtgg ataaacctgt
    16501 ggatgttgtg cataactgtt ggggaaatct ttttaacaaa aatagccaaa ataaacatat
    16561 agacgaaaaa attgattata ataaacaaag tgagcatgaa gaccaaagga gagaaaacat
    16621 gaatatcaaa attatctcag taggaaaatt aaaagaaaaa tacctgatcc aaggtatcaa
    16681 tgagtatgtc aaacggctga atgcctatgc aaaaatcgaa ttgatcgaag ttcctgacga
    16741 aaaagccccg gagaatttaa gcgaagcgca aatgagacaa gtaaaagaaa aagaagggga
    16801 acggatctta gcgaaaatca aagaacaaga atacgtctat gccttagcaa ttgaagggaa
    16861 aaatcctaca agtgaatctt ttgctaaaca aatcgaccaa ttaggtatcc aaggaaaaag
    16921 ccaccttgtt tttgttatcg gcggctcttt aggactcagc gaagccgtca tgaaaagaag
    16981 caatgctcag atctcttttg gaaaaatgac gtatccacat caattgatgc gtctgatcct
    17041 tgtcgagcaa atctaccgtg ccttccggat caatgcgggg gcgccttatc ataagtgatg
    17101 cggtttttat cattgatcct aaaaatctta gcaagacaaa aagtacgtaa gaaagtaaag
    17161 actaaatact tcggaggaac aaatgaataa caatgatagg ttattgcgat tgcgttatgc
    17221 gattgatttg aaagatacgg atttgattaa agcttttgag ctaggcggtg taccactgac
    17281 taaagaagaa gctcaggcgg tactgaccaa agtacaggat aagaacaaag ataatgcaga
    17341 aaataatgtg tatgaaaaaa ccatcaacaa ccaagttttt gacgcattcc taaatggcct
    17401 gattctcttg gcgcgtggtc cgcaaaaaga taagccgata gtagacacgc cgcaaaaaag
    17461 caaggaaatc aaatatatca acaatgtgtt gctaaaaaaa ttaaaaatcg ccttgtctat
    17521 gaccactgat gatattttag acgtttttgc tgaggcagaa atctatccat caaaaggcga
    17581 aatcggggca tttttgagaa aagaaggcca acgaaatttt aagccttgcg gagataaata
    17641 tatgcgtaat tttctcaagg ggttaggaat ttataatcgg agaaaagtat aaagaaattt
    17701 catgagaaat caaaacaatg gttgttttga tgagagctag tttttgtatg agattaaaat
    17761 attatcttaa tcgaggagct ggattggttt tatgaatgat gaatataaga atgatgaaga
    17821 taagatgctt tttgaagaga tagagaatcg atgtaggctc aattttgagc ttcggggaaa
    17881 gatgtcttta atacaacaaa aaaagtattt agcgaataaa tcagagttta ctctgggaca
    17941 tgttgaaaaa ttaatctcgg attggattag tagtagaagc gaatttacaa aaataaaaca
    18001 gccgattaaa tttgacatga aaaaactttt gttaaataag agtgagatag gtaatagaga
    18061 tcaatatatt agagctaaag ggcaagaaat aattgattca ttaggtgaaa tgaggtcata
    18121 caattatttg tatgtaacac atagagctga tggaatggtc attactgtgg ggaaatcttc
    18181 ttctaatgat atttttttag atggagatct tttttatcaa ctcaatacaa atcacttgtc
    18241 tggcactgaa aatataattt taaggacgga atatggcaat gaaatatttg caaaatatga
    18301 tgagatttta aaaaattatt tggactgggc ttggataatt ccggttgagt caggagatgc
    18361 taagaaactg gagagattat tgggcgatga actaattaat aaaaaagtac caattttaaa
    18421 ttattattcc catagacaat aaaaggctat ttttcatgat aatactagtt ataactgtaa
    18481 aaatggaaga aaatctggga aagaagtttt taaataagtg tattgttcga ttttaataaa
    18541 taataagcaa taccatccgt ctgtgttgcg caacatcgta ctacaatgaa tattttttca
    18601 caaaaagaaa atcatccatc gaatgaatgg aaggttttag tatacacgag gttaatgccc
    18661 cctcttttat aatcagagga gtgaggtttt ttctatgaat gtttctattt tcttattgct
    18721 agtctagttt ctcttacgat gaaattggga ttctattcca gtttttattg atttgctgat
    18781 aaaataactt gaatggtgga tataaacatg ataaataaga gaagaataac aaaacaaggt
    18841 tgtgaaatga ttctcaaatg aatataatag aaagaaaaag gaggaagaat caatgaaaat
    18901 agcaattgca ggcgctggcg caatgggaag tcgctttggg ttaatgcttc accaaagtgg
    18961 gaacgaagta ttattaatcg atggctgggc agaacatgtc caacaaatca aagagcatgg
    19021 attacaagca aattttaatg gtaaagaggt agaagcaaaa ctaccaatcg ttcttcaatc
    19081 cgaagtagaa aaagaagatc aagttgatct gattattcta tttaccaaag cgatgcagct
    19141 ggaaaaaatg ctgcaggata tccaatcatt aatcaaaaaa gatacagagg tcttatgtct
    19201 attaaatggt atcgggcatg aagatattat tgagaaattt gtaccaatgg aaaatatcta
    19261 tattggaaat accatgtgga cggcaggtct tgaaggtcct ggtcaggtca aattatttgg
    19321 aagtggttca gtagaattac aaaatttagg tgatggaaaa gaagcagctg caaaaaaatt
    19381 agcagacaaa ttgtctgaat caggattaaa tgctcatttt tctgacaata ttcactattc
    19441 tatttatcgc aaagcatgtg tgaacggaac aatgaacgga ctatgcacaa tcttagacgt
    19501 taatatggct gagctaggaa aaacatcgac tgctcataaa atggtggcga cgattgtcaa
    19561 tgagtttgcc aaagtagcag cagtagagaa gattgaacta gatgtcccag aagtcattgc
    19621 acattgtgaa tcttgttttg acccggaaac aattgggtta cattatcctt caatgtatca
    19681 agacttgatt aagaaccatc gattaacaga aatcgattat atcaatggcg caatttctag
    19741 aaaagggaag aaatatggtg ttgcgacgcc ttattgtgat ttcttgactg agcttgtcca
    19801 tgcaaaagaa gatagtttga acgtaaaata aaggagagat tgaatggaaa cttcacaaaa
    19861 aatatcaccg aaaatttttc tgaataaagt cctcgctggt acagcaacag gaatcattat
    19921 tggattgatt cctaatgcag tattaggtgc cattttaaaa tattttagtg cgtatccctt
    19981 tgcggtaacg atcgcacagt tagcagttat ttttcaacta gcaacacctt tgatcattgg
    20041 tggtttgatt gccttacaat ttggatttga tccgatgaga atgatggttg tagccggagc
    20101 ttcttttgtt ggttctggtg tagtcaaatt cacacctgat ttgggtgaaa cgggtgttta
    20161 cgttggtgct ggtacaggcg atattattaa tacgatgctg acagcatcga ttgctgtcgg
    20221 gctgatttta ctgattggaa atagttttgg gtcagtgtcg attgtgttaa caccgattgt
    20281 tgtaggtgta ggtgcaggtt tacttggttt ttatttattc ccttttgtaa cagcaattac
    20341 ttctgcaatt ggatcattga ttaataactt cactacctta caaccgctgt taatgtctat
    20401 cttgattggt tgttcctttg cctttttgat tatctctcct atttcaacag tagcaatcgg
    20461 aatggcaatc caactagatg gtgtttctgc tggtgcggct gcgatgggcg tagctgcaac
    20521 cacttttgtt ttagtagtga actcttggaa ggttaataag tcaggtgtga caattgcgat
    20581 tgctttaggc gctatgaaga tgatgatgcc taatctattt agaaaacctg tgattcttat
    20641 tccttgttta gttagtgcag tgattacagc tattcctgtc gctctatttt ctatttcagg
    20701 tacgcctgct tctgctggtt tcggcgtggt cggacttgtt ggtccgttgg cttctttaga
    20761 tgcagggcta aatggctttc ttgttgttct ttgttggtta gtcatccctg tttttgcggc
    20821 actaattact caatttttat gcgagaaagt atttaaactg tatgatcgag aagaagtctt
    20881 caaattttta ggctagtaga ttttactaag aaattttcaa tcaatggatg cgtaagtgag
    20941 atgtcaacaa catctgactt acgttttttt gcctttttga gtaggagatt gatgctgaca
    21001 actgaactct tcagtgccat tcgatttttt aaaatgtaga agaaactcac ctttatgtta
    21061 ttgattgttc aaaaggcaac tagaataaaa atatgtgtta aaatgagaaa aaatttgatt
    21121 gttggatgta tcgaataatc actaaaaagt aggagaagaa aatgattttt ttgcaaaaat
    21181 atggttttta ttttttatta ttaggtgtga ttagtgattt atcgacaccg tatatccttg
    21241 gcctttttta tcccaaactg aatcaaatga ctaccgtaat tagtgtgttt ggtgacgtag
    21301 atagtccagt cagacgtgct ttcttggtct ggtcagttgt atctggactt ttttttgttc
    21361 tttcgttgcc tgcgttgtat cacctattta ttggaacatc aaaaacactt gcaatacttg
    21421 ctgttgcaac agttggttta tatgggatag gtgattgtat ttttactggg ttattcagta
    21481 tcaatacgaa tgaaagctct tggaatctat cgacttggat ccataatata ggttctgggc
    21541 taggctatgc aggctttcta cttttcccat tgttacttgt tctgctttat cgtcaaagtg
    21601 gtcaaggtac gcttagccat ttttatcttg ttttaacagt tattagttta ctaattgcag
    21661 gtctttacag attagcacgg attccgtcga ttagtcagtt tgccttcttt aagcaattgg
    21721 gcttctttca acgcttaagt tttttcttta actatttgcc gatgatttgt ttaagttttt
    21781 ttcaactacg aaagtagaaa aagcagtgtg aagctgatta agtaaaagaa tttctctatg
    21841 ggtaaccatt cgtgtggatg tgttcgacta agaatagaga tgaggagagc gaaaagcaag
    21901 ttagtcttaa cttgttatgc gttgtgctaa atttcaggta gtcatttttt gaatgaataa
    21961 gaagaaaact tgaaagaggg tgacttcatg ttagatgtgg agcagatgga aatagacctt
    22021 tataatatta ttttaaatcg aaatacaaga gaatgggaaa gaacacaatt tgaagaggca
    22081 aaagaagacc tagcaaaaat gtcgataaag caggtgatag aaaaactaga ggtcaacttg
    22141 cgtccgctag cactacgaga taatttgaca ccagatgcga tggattttta tttaagaatc
    22201 acaggggagt cagcgagtgc aaaggcttat gattatgcca aacataagat aacttcaccc
    22261 ttataccaag aacgggcgat ttttgccggt ggctgtttct ggtgtatggt tgagcctttt
    22321 gaaacaaaac caggtgtaat ctcagtgtta tctggttata caggtggaga gaccaaaaaa
    22381 ccaaattacg atcaagtcag tggtggatat actgggcatg ttgaagctgt tgagatcata
    22441 tttgatacac gtcaaataac ctatgaagag ctgttggaga tttattggca aatttcagac
    22501 cctacagatg cctttggtca atttcaagat cgggggaaac aatatcgttc aattctattt
    22561 gttgttaatg aaaagcaaag gcaattggct gaaaaatcaa gagaacaact gattcaatct
    22621 aaccgattta gccgtcctat cgttacagag attcgccaag caaagacttt ttggccagct
    22681 gaaaatcatc atcaacaatt ttatcagaag caacctaaaa gatataaaaa gatcaagcat
    22741 tcgcatcagc ttcatttatt ttttcagcga catagataga ggaaggttgt ggcgcaaagc
    22801 gttcagctcc gacaaaataa tgagcaactg acaaaaatag cttttcatat ttttggacag
    22861 ttatgaatta tttcgaggag ttgctttgag aacaccgagg aaaaggttgt ggcgcaaagc
    22921 attcagcttc aagtcgcaag gaaacaggag gtatgacaag tcttttgtca caactcctgt
    22981 ttttttacta gcctttattt ccatgtgaaa gagaattcga attgtagaat cttgattttt
    23041 ctatggaatt ttgtagagaa tcatcttgat aattaataga cacctaatcg aattccacat
    23101 taatgatgaa ctaaattccg tataaaagcg caaacaataa cta
//
LOCUS       DAFFFQ010000043        23012 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000043
VERSION     DAFFFQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.043"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(260..1492)
                     /locus_tag="LQB69_001720"
     CDS             complement(260..1492)
                     /locus_tag="LQB69_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL3525887.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1747..2316
                     /locus_tag="LQB69_001721"
     CDS             1747..2316
                     /locus_tag="LQB69_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3525888.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2494..2934)
                     /locus_tag="LQB69_001722"
     CDS             complement(2494..2934)
                     /locus_tag="LQB69_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL3525889.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3092..3856
                     /gene="map"
                     /locus_tag="LQB69_001723"
     CDS             3092..3856
                     /gene="map"
                     /locus_tag="LQB69_001723"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL3525890.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3888..4811
                     /locus_tag="LQB69_001724"
     CDS             3888..4811
                     /locus_tag="LQB69_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL3525891.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4887..6026
                     /locus_tag="LQB69_001725"
     CDS             4887..6026
                     /locus_tag="LQB69_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL3525892.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6019..6819
                     /locus_tag="LQB69_001726"
     CDS             6019..6819
                     /locus_tag="LQB69_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3525893.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6819..7646
                     /locus_tag="LQB69_001727"
     CDS             6819..7646
                     /locus_tag="LQB69_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL3525894.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7624..8358
                     /locus_tag="LQB69_001728"
     CDS             7624..8358
                     /locus_tag="LQB69_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3525895.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8458..9324
                     /gene="rfbA"
                     /locus_tag="LQB69_001729"
     CDS             8458..9324
                     /gene="rfbA"
                     /locus_tag="LQB69_001729"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL3525896.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9338..9910
                     /gene="rfbC"
                     /locus_tag="LQB69_001730"
     CDS             9338..9910
                     /gene="rfbC"
                     /locus_tag="LQB69_001730"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL3525897.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9932..10960
                     /gene="rfbB"
                     /locus_tag="LQB69_001731"
     CDS             9932..10960
                     /gene="rfbB"
                     /locus_tag="LQB69_001731"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL3525898.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11058..11909
                     /gene="rfbD"
                     /locus_tag="LQB69_001732"
     CDS             11058..11909
                     /gene="rfbD"
                     /locus_tag="LQB69_001732"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL3525899.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11943..13976
                     /locus_tag="LQB69_001733"
     CDS             11943..13976
                     /locus_tag="LQB69_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525900.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14020..15299
                     /locus_tag="LQB69_001734"
                     /pseudo
     CDS             14020..15299
                     /locus_tag="LQB69_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15509..16315
                     /locus_tag="LQB69_001735"
     CDS             15509..16315
                     /locus_tag="LQB69_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525901.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16327..17547
                     /locus_tag="LQB69_001736"
     CDS             16327..17547
                     /locus_tag="LQB69_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525902.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17537..19123
                     /locus_tag="LQB69_001737"
     CDS             17537..19123
                     /locus_tag="LQB69_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3525903.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19162..21300
                     /locus_tag="LQB69_001738"
     CDS             19162..21300
                     /locus_tag="LQB69_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3525904.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21668..22651)
                     /locus_tag="LQB69_001739"
     CDS             complement(21668..22651)
                     /locus_tag="LQB69_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL3525905.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
BASE COUNT         7217 a         3934 c         4747 g         7114 t
ORIGIN      
        1 cgagtctctt ctttctgagg agagacatca agagtcgctg cgctactgct cctgacctct
       61 ctcctcagaa agaaaggcat ggcagttagg ttcacaacgc aaaacattgt gcttttgatg
      121 aggttgaact tttacacaag attgtggact tgactaaaaa aacggtgggg ttgtttagcg
      181 aaataaagga tctaagccaa tagcttagat cctttattct atgctttata ttattttttg
      241 catgtgtgcg aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc
      301 catcttcacg gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg
      361 tttgacttct attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt
      421 tcacactgaa ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata
      481 atgtattgaa aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga
      541 cttctcctaa acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc
      601 cttgttcggc agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga
      661 tgatggtgcc agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac
      721 tgtcaggtgc tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag
      781 cattatagct tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg
      841 taccgggagc agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt
      901 ttgctaattt ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag
      961 tcgttttgaa aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga
     1021 agacttttgc tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg
     1081 cttgagttgc cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta
     1141 ctcgatggct atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc
     1201 ttgctccttt ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa
     1261 tacgatccgt tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa
     1321 cgataacctc agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg
     1381 cctgttccac gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt
     1441 ctgcaattaa gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa
     1501 ttcctcctca ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa
     1561 caatgaggct ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat
     1621 gttactactt gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa
     1681 aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta
     1741 aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa
     1801 gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat
     1861 tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt
     1921 tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat
     1981 acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat
     2041 cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact
     2101 tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc
     2161 gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca
     2221 agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct
     2281 atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc
     2341 agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca
     2401 tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga
     2461 ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc
     2521 gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc
     2581 ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca
     2641 gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc
     2701 caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta
     2761 tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt
     2821 acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc
     2881 gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg
     2941 cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa
     3001 ataaaaccta gagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa
     3061 tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat
     3121 tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt
     3181 tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag
     3241 tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag
     3301 tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt
     3361 ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta
     3421 tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt
     3481 gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat
     3541 ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt
     3601 tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt
     3661 gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa
     3721 aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca
     3781 atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga
     3841 agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga
     3901 acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca
     3961 ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat
     4021 ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg
     4081 tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc ggtcctgcaa
     4141 tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt
     4201 tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg
     4261 tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt
     4321 tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg
     4381 ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt
     4441 ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta
     4501 atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga
     4561 tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt
     4621 atcagatcat aggaagtttt attgtactca tgctttggct taattttgct gctacgatca
     4681 tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca
     4741 aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata
     4801 aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat
     4861 gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt
     4921 ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa
     4981 caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg
     5041 gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta
     5101 tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag
     5161 tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt
     5221 ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg
     5281 gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca
     5341 cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg
     5401 gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc
     5461 cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc
     5521 cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc
     5581 tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca
     5641 tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt
     5701 ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc
     5761 ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg
     5821 tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg ttgtttgctg
     5881 caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa
     5941 cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga
     6001 aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg
     6061 acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat
     6121 gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga
     6181 ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga
     6241 tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt
     6301 aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa
     6361 catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac
     6421 ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg
     6481 gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat
     6541 gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt
     6601 aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt
     6661 ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat
     6721 ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg
     6781 atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag
     6841 tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag
     6901 cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa
     6961 atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag
     7021 cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc
     7081 tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta
     7141 cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc
     7201 tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag
     7261 ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga
     7321 ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag
     7381 tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg
     7441 ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc
     7501 gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa
     7561 gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct
     7621 catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat
     7681 ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat
     7741 gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac
     7801 gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat
     7861 tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat
     7921 gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag
     7981 ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg
     8041 tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat
     8101 attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca
     8161 gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta
     8221 agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat
     8281 tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga
     8341 aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa
     8401 caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg
     8461 aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca
     8521 tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg
     8581 atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc
     8641 aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa
     8701 agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt
     8761 gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa
     8821 cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa
     8881 cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct
     8941 gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt
     9001 tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat
     9061 aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg
     9121 cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa
     9181 aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc
     9241 gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat
     9301 ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt
     9361 aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga
     9421 aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa
     9481 ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc
     9541 tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat
     9601 ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca
     9661 tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt
     9721 gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt
     9781 tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga
     9841 aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg
     9901 agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc
     9961 cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt
    10021 cactgttttg gataaactga cttatgcagg aaacaaagag aacttggctg gacttcctag
    10081 cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt
    10141 aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa
    10201 agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg
    10261 ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc
    10321 attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac
    10381 accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa
    10441 agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg
    10501 tccttatcag catattgaaa aatttattcc tcgccaagta acgaatatct taagcggtat
    10561 tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca
    10621 ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg
    10681 tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggtca
    10741 accaaaagat gcgtacgacc atgtaaacga ccgtgcagga cacgacttgc gttatgcaat
    10801 cgattcgaca aaattacggg aagagttagg atggaagcct gagtttacga acttccgtga
    10861 tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa
    10921 agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagagaaa atgtcactgt
    10981 attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc
    11041 aaggagcgga taatcatatg tttttattaa ctggaggaaa tgggcaacta ggtacagaat
    11101 tgcgccattt attagatgaa aaaaatctgc aatatgtttc aacagatgca aaagacttgg
    11161 atatcacaga tgccgagaaa acaatggctt acatcacaga attaaaacct gatgttattt
    11221 tccattgtgc agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg
    11281 agaagatcaa tgttgatggt acaagaaatg tagcacttgc agcaaaagct gtgaatgctc
    11341 aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg
    11401 aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa
    11461 aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga
    11521 tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga
    11581 ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg
    11641 tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct
    11701 gttcttggta tgaattcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg
    11761 ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga
    11821 aaaagacaga agagttagga tttgtcatac caacttggca agaagcactt gcgcaaatgc
    11881 tggaagctgc caaaaaaatg aataagtaat ttccttttat caaatagaat ttggagcatg
    11941 atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aaccaggcag
    12001 attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca
    12061 tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg
    12121 tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc
    12181 ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttc
    12241 cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc
    12301 ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa
    12361 gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat
    12421 tcgaagtact gatgatatta acgaagagaa aaaaagctct atcgctaatc ggtgcatttg
    12481 cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt
    12541 tctttacttt gggactgatg actgcttttt atcattattt ctacaatcat gataaaaaat
    12601 ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga
    12661 tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc
    12721 ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg
    12781 cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt
    12841 tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga
    12901 ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt
    12961 cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc
    13021 catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga
    13081 ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa
    13141 tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt
    13201 gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt
    13261 ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga
    13321 aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga
    13381 ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt gccgccttag
    13441 ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa
    13501 aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt
    13561 ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac
    13621 gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt
    13681 attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt
    13741 tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga
    13801 tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt
    13861 tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg
    13921 attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg
    13981 ctagctgaga ataatcctga aatgttagta gaaaggagag tggagataat tcaagcaatc
    14041 gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttact gacgctagtc
    14101 ttttttgttt ggacagcagg gttaggtttt ccagcaactt tgtttatata taacaatagt
    14161 tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa
    14221 ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt
    14281 cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt
    14341 tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc
    14401 ttttcggtct tcctcggaat cacgtttatc gtgtatttac agagatccaa gtaccagaag
    14461 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
    14521 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
    14581 gatggtatgt acgaatcatc ggtgcagtag cagttgttgc agcttttctc gggatttggg
    14641 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
    14701 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ttacacggtt acgattcttt
    14761 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccggtc
    14821 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caaattttac
    14881 taggaatgaa aacatttatc ttccaacgag gaaaccaatt tttaggaaac cacaatagtt
    14941 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    15001 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    15061 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcataac
    15121 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    15181 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    15241 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    15301 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat
    15361 tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat agaaaattaa
    15421 gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact tgactgtaat
    15481 tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat ttatgaagga
    15541 catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca aatcaagata
    15601 tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta ctgtattgat
    15661 ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa aatacccatt
    15721 tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg catttgcatc
    15781 aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat tcaatattcg
    15841 tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct tcattctgat
    15901 tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt tacagctgat
    15961 ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga cggcatcgat
    16021 ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg
    16081 gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa gcatattgaa
    16141 attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg
    16201 gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag taatgttcgt
    16261 aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc tgtaaggaga
    16321 atagctatga cagaatatgc aattagacta aaagatatca caaaatctta taatatgtac
    16381 gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat
    16441 gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc
    16501 gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg agtactgaca
    16561 ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga actaggatca
    16621 ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat ggttctagga
    16681 ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc tgacatcgga
    16741 gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg tctagctttt
    16801 gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt ggcagtaggc
    16861 gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa ctcgggaaag
    16921 acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga tcgttcctat
    16981 tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac gacgaattat
    17041 gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa ccaagtagaa
    17101 cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga taaagacaaa
    17161 aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac ttatgaagta
    17221 aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt cgacaatttt
    17281 tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa aaaaggagag
    17341 aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata ttattttgac
    17401 gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa gtacatgaac
    17461 ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca taaatgggaa
    17521 gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac aaagcagtgt
    17581 gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg gtcccggaaa
    17641 cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca ttgttgagtt
    17701 agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt atggtgatat
    17761 cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg tcttgtttgc
    17821 ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca gagaattttt
    17881 gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt ctgtcatgat
    17941 cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg aaacccataa
    18001 ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat tattcattaa
    18061 tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga
    18121 attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag tctaccaata
    18181 ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc cacaaaattc
    18241 taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa caatccaaca
    18301 aataccattc aataatttca caggggaaaa tgagacacta agtttccccg tatcagaaac
    18361 aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat tcagcgcaga
    18421 agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa cagtaaatga
    18481 ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata tttctgggaa
    18541 agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa caatgaaaga
    18601 attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg agaaaaatga
    18661 cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag accatacgtt
    18721 aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga caataaaaca
    18781 tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag aactttctat
    18841 atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca tcggatgggg
    18901 aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc aatcgccatt
    18961 tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc tcgcaaaagg
    19021 aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat tcttagtgga
    19081 agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga ttaacttata
    19141 aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc
    19201 actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga tgaagtgacc
    19261 aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc
    19321 gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag cggttttaag
    19381 gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac agcgagacat
    19441 caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg cattgaatct
    19501 tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag gactcatggc
    19561 gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat cgactatgca
    19621 gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga agcacaagga
    19681 tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga aatcaaatgg
    19741 ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga actatgtatc
    19801 agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta tcaagcaaaa
    19861 gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct ggcaactaat
    19921 tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga tgatgaatta
    19981 ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga actggatgtg
    20041 atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc gcatttcaaa
    20101 gcagactggt cgcctgatac attgatggga aataattata tctctcactt aggcgtatat
    20161 cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg ttctcaagat
    20221 tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta ccacattgac
    20281 cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg cgaagccaaa
    20341 agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag aagaaacatc
    20401 aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta tgacgtcttg
    20461 caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga tctaaaaact
    20521 tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat tattgcggat
    20581 aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca tcagctgaaa
    20641 gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat taacaattta
    20701 gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac agaagtgatt
    20761 gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat cggctgtgta
    20821 ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt attaggaatc
    20881 ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg ttatttcggc
    20941 cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat ggtcaaggca
    21001 gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt caacgatgtt
    21061 gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca tcaagcagaa
    21121 ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa acaaagacgt
    21181 tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc gcatgatcca
    21241 ttttacaatg ataatttaac aaaagaagga atcggcgact tttcgttgag accagattaa
    21301 actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt ttatagtttt
    21361 ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg ggacagaagt
    21421 gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    21481 tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt taaagaatga
    21541 gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc gccaatagat
    21601 acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata ttttatttta
    21661 atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc actctatcaa
    21721 ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta gcgtcaatat
    21781 aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa tctaataccc
    21841 accataaata gccatatctc atattttgta agtcttctaa agcactggtt ctaggtttga
    21901 acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc cctttattta
    21961 ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca tatcctgccg
    22021 tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta tccattgtaa
    22081 actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt atccctatag
    22141 gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc tgatacaaca
    22201 agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt ataccttttc
    22261 ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag acttttgtcc
    22321 atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg attgtaatag
    22381 cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct aatactttat
    22441 catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa gatacaatac
    22501 ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc cattcatctg
    22561 tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt tgtcgaaaac
    22621 caataaaatc atgcaactct tgtttattca taagatatta cctcactttc ttctataagc
    22681 ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac aggtattctt
    22741 atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg ttcactttat
    22801 gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca ttagtgaaaa
    22861 tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt gtataaagct
    22921 ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt tttaaaggtg
    22981 actatggtaa gataatagag atattatttt gt
//
LOCUS       DAFFFQ010000044        22943 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000044
VERSION     DAFFFQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.044"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..1235)
                     /locus_tag="LQB69_001740"
     CDS             complement(<1..1235)
                     /locus_tag="LQB69_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL3525906.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQK"
     gene            complement(1228..2820)
                     /locus_tag="LQB69_001741"
     CDS             complement(1228..2820)
                     /locus_tag="LQB69_001741"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL3525907.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3329..4054)
                     /locus_tag="LQB69_001742"
     CDS             complement(3329..4054)
                     /locus_tag="LQB69_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525908.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4208..4534)
                     /locus_tag="LQB69_001743"
     CDS             complement(4208..4534)
                     /locus_tag="LQB69_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525909.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4571..4936)
                     /locus_tag="LQB69_001744"
     CDS             complement(4571..4936)
                     /locus_tag="LQB69_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL3525910.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4973..5188)
                     /locus_tag="LQB69_001745"
     CDS             complement(4973..5188)
                     /locus_tag="LQB69_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525911.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5317..5652
                     /locus_tag="LQB69_001746"
     CDS             5317..5652
                     /locus_tag="LQB69_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525912.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5728..7725)
                     /locus_tag="LQB69_001747"
     CDS             complement(5728..7725)
                     /locus_tag="LQB69_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525913.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7823..8098)
                     /locus_tag="LQB69_001748"
     CDS             complement(7823..8098)
                     /locus_tag="LQB69_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525914.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8330..9064
                     /locus_tag="LQB69_001749"
     CDS             8330..9064
                     /locus_tag="LQB69_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3525915.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9103..10245
                     /locus_tag="LQB69_001750"
     CDS             9103..10245
                     /locus_tag="LQB69_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3525916.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10340..11731)
                     /gene="sufB"
                     /locus_tag="LQB69_001751"
     CDS             complement(10340..11731)
                     /gene="sufB"
                     /locus_tag="LQB69_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL3525917.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11736..12206)
                     /locus_tag="LQB69_001752"
     CDS             complement(11736..12206)
                     /locus_tag="LQB69_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL3525918.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(12193..13428)
                     /locus_tag="LQB69_001753"
     CDS             complement(12193..13428)
                     /locus_tag="LQB69_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3525919.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(13425..14711)
                     /gene="sufD"
                     /locus_tag="LQB69_001754"
     CDS             complement(13425..14711)
                     /gene="sufD"
                     /locus_tag="LQB69_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL3525920.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(14724..15497)
                     /gene="sufC"
                     /locus_tag="LQB69_001755"
     CDS             complement(14724..15497)
                     /gene="sufC"
                     /locus_tag="LQB69_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL3525921.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(15694..16542)
                     /locus_tag="LQB69_001756"
     CDS             complement(15694..16542)
                     /locus_tag="LQB69_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL3525922.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(16566..17252)
                     /locus_tag="LQB69_001757"
     CDS             complement(16566..17252)
                     /locus_tag="LQB69_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525923.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(17245..18279)
                     /locus_tag="LQB69_001758"
     CDS             complement(17245..18279)
                     /locus_tag="LQB69_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525924.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(18342..18598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18674..19012)
                     /locus_tag="LQB69_001759"
     CDS             complement(18674..19012)
                     /locus_tag="LQB69_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL3525925.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(19014..19370)
                     /locus_tag="LQB69_001760"
     CDS             complement(19014..19370)
                     /locus_tag="LQB69_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL3525926.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(19376..20563)
                     /locus_tag="LQB69_001761"
     CDS             complement(19376..20563)
                     /locus_tag="LQB69_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL3525927.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(20754..21599)
                     /locus_tag="LQB69_001762"
     CDS             complement(20754..21599)
                     /locus_tag="LQB69_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3525928.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            21833..22798
                     /locus_tag="LQB69_001763"
     CDS             21833..22798
                     /locus_tag="LQB69_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL3525929.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         6607 a         4742 c         3878 g         7716 t
ORIGIN      
        1 atcttttgta agaagccttt tttcgtttcc ttcagcaaat ctaacttacg ttgatgaaga
       61 gcgatcgtgt cgtcgagttg tttgaagaaa gcgccgattt tttgttgttc ctctttattc
      121 ctagtaaaag gaaacttgat attagcgaaa ttctcataga ataagtattc tcgtacgctt
      181 ccttcagtgt tgcgcttgac ctctttgata aatgttggtg acttgataaa ctgaagtatg
      241 aactcattat ctaagtcctc gctcattttc acaactacat aaagagagct aacaatgacc
      301 gtcataccaa gattgttaaa tgcaattgat cctacgttta ctcttgctgg attataggcg
      361 aattcatttg gttcaacgaa tttataattt gttttttcta aatcatccaa cctactacca
      421 tcaaactgtt ctgtttgaga aattaaacct gctttattac ttaccgaata ggcagggaag
      481 tctaaatctt tcgtgttctt ttttccagtc ttcttactaa actctttgaa cttacgctgt
      541 tcccaatctt ccgtaaatcc aggaaaccga atttctggca cttttgctcc gtttttaggg
      601 aacatttttt gtaagaatcc ttttttcgtt tcctttaata aatctagctt acgttgttga
      661 agagcgatag tgtcgtccag ttgtttgaag aagttcccaa ttttcttttg ttcttctaat
      721 attggaaatt gtactatcca ttttttcact tctgacattg gtacgagatt tgtaattgct
      781 gaacctggat tagctcctgt gagttttctt tgcataaaat ctgcacgaat aaattgaata
      841 aaatattctg aatctgtgat ttctttattt attctaagtc taatcaaagc ctgattgaat
      901 acacccggtt ttatttgctt tggtactctt gaaattctcc caattgttcc agcgccactc
      961 atgataaaat cgcctggttc taatttaaat ttgattaatt cattatattt tttttcagta
     1021 attttatatc ttgtttcgta attatcataa atagcatttt gttgttcata gacaacaaaa
     1081 ttgctattag atacaaagaa ttctttcttt aaggcactcc caaatggtcc cctccttact
     1141 ccatcttttt tatcaagtaa gcattcaaac ttacgctgtt cccaagcttc cgtaaatcct
     1201 gggaaacgaa tttctggttg tatatcatta ctcatgtttg aacacctcca gcgcaccttg
     1261 taaccacgct tcattttcag gtgttgtttg cagggaagag atagcttcta aaagttcttt
     1321 ttctagcact tgctgttctt gtcgtatctt cttgatgtca tttccgacat gaaccatatc
     1381 aattggttct tcttcttcaa acgtatctac atagcgtgga atatttaagt tatagtcatt
     1441 ttctttgatt tcatcaaagt ttgccaaatg agcgtatttc tcgacatctt ttctttcagc
     1501 ataggtttcg aggatttttt gaatgttttc ttccgataat ttattctgat tttttccttt
     1561 gacaaattct cgactagcgt caatgaataa tacgtctcga gtttggcggt tctttttcaa
     1621 tacaatcacc gtcgttggaa tcgatgtccc gaagaacaag ttcgcaggca taccgatcac
     1681 agcataaata ctgccgtctt ctagtaattt ttgacgaatg accccttctg cagcgccacg
     1741 gaataaaact ccgtgtggca agacgatggc catagtccct gtttccttca aatgatagaa
     1801 accatgaaga agaaacgcaa aatctgcttt ggattttggc gctaattttc cataacgatt
     1861 aaaccgagaa tcgtctaaga aggtcgtatc tgctgaccaa ttagccgaat aaggtgggtt
     1921 cattactact gcatcaaatg tgtaaggttc atctgttggc caatccttat tcaacgtatc
     1981 cccattgcgg agattcattt cttcggcatc taccccatgt aaaatcaagt tcatttttgc
     2041 tagattgtat gtcgttgtat ttaattcttg cccatgatat ttaacgttat ctgggtgagt
     2101 taagtagtta cgcacattta acatcaatga acctgaaccc atggttggat caaaaacact
     2161 aaagaatcgt cgttcttttt gatccaacgt cacgatttgt gccatcatat cagaaaccat
     2221 atgaggcgtg taaaattctc cggctttctt ccctgcttct gaagcaaatt gactgatcag
     2281 aaattcataa gcatccccga tcacgtcccc atcgtgacct aaaacttcca cgtcattcaa
     2341 tttcttaatg acttcggtaa tcgtcacatt tcgttgttgc tcatctgttc ctagtttttt
     2401 tgattggaga tccacatcat caaataaccc attaaattga ttgtaggtag atgccaattg
     2461 cacaaaggct ttgtttagat cattcaattg gaacgttgct tgttttgctt gatccgctaa
     2521 gacgttgaaa agatactctg gcgaaatcgc ataccccaaa atatcaacga ttgttgcaat
     2581 cagatcttct tttgattctt catctgaaag taattcgcga tacaacatcg tttgtttgct
     2641 taccgtatca tattcctcta aggattcatc tgctaaaaga accacttgtt ccaacaaacg
     2701 gtcggataaa tatttataga aaatcagtcc caataaataa tttttatatt ctgacgcatc
     2761 cattttgcta cggagattat ctgccgcact aaataatttt tgatttaatt ctgcacccaa
     2821 aaagatacac tcctatctct tatttaaagt tgctatatac cctcttattt tagcatactc
     2881 ccttttagaa aaagcaaaaa aaaaagaaag aaacttattc ttgctaataa actacgcgaa
     2941 aggagtctaa cgttgtttga tataacttag actgatcgtt aacttatttt ttatatttct
     3001 acatagttat ctttagaacc atttcagata catatgaaac aattacatat gattttttca
     3061 taaaaaaaca gtactgtcta gtagtataaa atatacaaat ttcttaaaaa ttttttcact
     3121 aaaatcacta cctgaaacag tctaaaacac ttatatatca acgtttaatg aaactatctc
     3181 cgtttcattt cagatacaaa taaatatagt cgggctccaa atccgaaaaa aaataccccc
     3241 agtagcttat cctaagctac tggagatgta taagtacggt catataacaa cggggtaggg
     3301 taaataaatg aaaaagatgg aagaatcatt attcaataac ttttttaaat acttcgttgt
     3361 aatcagttac ctgtccgttc ttaaatacca cagaataatc ttttttccca ttttcaagta
     3421 accgaagtgg aatatcttta ccaactttct ctgtcactgc attactgata cctgttagat
     3481 tagctaataa atctgataaa gattgagcat ctgtagaaat ctctgtatca gtatttatcg
     3541 tgattccttt atcttcattt acaagtttcc aagtaggtgg tatagctgtg tatgtttcta
     3601 caaatgaatt aagtttttca ttaagtgact tctcttttat atttccatct aaaaactctg
     3661 tatatatggc ttgagctata ctaaaacaaa gctggccttg ttctaataat tttacatcat
     3721 tagttagtaa agctgtgttc acgttatcaa tcgcttcaag aagagtatct ttggtgtccc
     3781 taacagcttt aggtgtgcct tctggaattt ccctacttga tacctcgtcc attttatctt
     3841 ccataaaccc cttaaaatca tatctttctc ccaaatccat agaagttttt gagaaagctt
     3901 tcatttctga gtctatttct tcaataatat ttaacatgtc tgatacatat tgtttaggct
     3961 gattcgtttt taatctatat gtggtaaata tctcaattcc tccaattatc aataaaattg
     4021 ccaaacaaat ccataataat tttttctttt tcatttttcc tcctatacac tgctgttata
     4081 acagtgacta atgtaaagat tttcccagca atcatcagac tattatttac agggaatcaa
     4141 aaaaactcct tagtaagctt acacctttag gagttcctgt ttgaacggta tgcataatac
     4201 atttaaatca atttaacatt aacacctcag tatatggtat ctctacatat acacggtctt
     4261 tttttggata gtactcgaca tttatattga tagcacttcc attgtctaac gtagctttag
     4321 cccggtacgt gttatcacca ttatcatgag tgatcgtcac atcatcacat gtccgattta
     4381 agttatactg ttcttgtaaa atctgagtta ccaatggtac agatgacttc tctagtccag
     4441 atgaacccgt cattcttgaa aaaattatac ctataatagc caatacaacg atacctataa
     4501 tgacaaactt cttattcata tttactttat tcaacttttt atctcctatc tccaaaatga
     4561 ttttctctca tcagtaactc attgacatcc ccaatagtac accaaagatg gctccagcta
     4621 cccctgctat catcaaaatc gtacccattt gttcatcttt ctctcgatat aagtaaccca
     4681 taataacagc aacagcacca aataccacag gaataaatgc taatgatact accatagaaa
     4741 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
     4801 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
     4861 tttcttgtaa tgctccgcaa aactggcaat acttactttc atttgttatt tctttaccac
     4921 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agctaactgt
     4981 ttactttatt aaatacatca gaaaacagct catttattgg tacacttaat atctcagaaa
     5041 gcattttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
     5101 gctgtgtaac attcattttt ttggctaact cagtttggga aagtcctttt tcttttctgt
     5161 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
     5221 atatgatgta tttcaactca taaaatatat aaatacttca aatgtcatta caaatacaac
     5281 aacttgttgt actctactga gaaggggggt gtattaatga aacagaagca atatgcaaag
     5341 gtcattagag aaaagcgaaa agcactcggg tggacacaaa aagaattagc taaaaaaatc
     5401 ttctctacac aacaggcagt agcaagatgg gaaaattctg taacagaacc taacctcgat
     5461 agcttaacag ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt
     5521 gacatagatt atgaggaaga gtttttggct ctctatcgct ctcttagtac tgaagatgca
     5581 attcgaacaa ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta
     5641 ttgaaagatt gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa
     5701 taagattaag agctatggat aacctgtcta gtttaatata attgggtctc ttctattcct
     5761 tactctccgt ccatcatctc ttgttacact tgtaaattga taaataggaa ctaattcaac
     5821 tcctgataat atcaattctt ttattacttc atctatccct gcccctttcg ctagatatac
     5881 taaaaaacta ctgcttgctt tcacattgta tagtttctta atatccttag aagctcgttc
     5941 aagagtgaat ccttgtttaa caaagtagcc tctgttttct attaaagcat ttttcacgct
     6001 gttatccctg acatttcgta ggcgttcaca aacagtgtta ataagttctt tttctgtcat
     6061 tagatatgtc ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa
     6121 ttgtttttcc ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt
     6181 ttcgttaaaa catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt
     6241 ttccagaagt ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac
     6301 gtctaatcga tagacattgc ttctcttatg tttaaagttt cctaacgctt tctcttcctt
     6361 taacctattt aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat
     6421 caatcctata aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt
     6481 accatagtaa gaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact
     6541 ttccttacta tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat
     6601 ttcctctaga taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc
     6661 aaaaagtact tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt
     6721 ctctaatagc caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac
     6781 tccaataaaa ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc
     6841 agttttactg gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa
     6901 gtttttactt gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc
     6961 attaaaagac atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc
     7021 tttgtcgtca ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct
     7081 ccctgaatca ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt
     7141 aatatctaac atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa
     7201 aaacattcta ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta
     7261 ttcgtaaaca gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat
     7321 cttgtatttt tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt
     7381 atgaactaaa ttgatagata aaaccttttc tatgcttaag tatctactat catctttact
     7441 caaatctact accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac
     7501 tctggtttcc acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa
     7561 tgattgtgat gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc
     7621 taaacaccta tctcttacat cagttttttc tttttttgta actggcttct ctaataattt
     7681 ttcttgatct acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt
     7741 ataaaattct gttaaagact aaaacataca gataataaaa acaaactctt tattactata
     7801 tgttttagtc ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag
     7861 attttttaaa tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat
     7921 attttctaat cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat
     7981 actctagagt gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct
     8041 ctttcacttg ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat
     8101 ttccccctcc tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg
     8161 ctcaaaataa aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc
     8221 atcgacatac acataatacc tcgataaaaa aggggtgtca acagctttgt aaaatttatt
     8281 tacaacaccc cttttttgag gtatactaat tttaggaggt gtggtcaata tgataaaacg
     8341 tccaccaata aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag
     8401 aaagagtaaa aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat
     8461 tgacaaaaaa actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt
     8521 atcccgaatt gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga
     8581 agaatatatt ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt
     8641 tccagataaa aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt
     8701 agatacatgg ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa
     8761 caagacttgt aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga
     8821 agaattattc atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag
     8881 cagtatagaa gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga
     8941 catgtcttat cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca
     9001 aacgatttca tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat
     9061 ctagtggtaa gtactccttg taaactaaga ggagatgatt taatggcaaa aatatcaaag
     9121 tacaaaaaga aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca
     9181 cgaactggtg aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa
     9241 aaagattatg acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat
     9301 atgacatttg aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct
     9361 tcaacaatcg caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag
     9421 cttaaactat cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt
     9481 aaatatgaaa gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt
     9541 gcaatgcaat atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag
     9601 tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt
     9661 aaagattttg gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg
     9721 agaaagagtg aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta
     9781 catgtcaata aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa
     9841 acaagagctt ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac
     9901 tggaaattac agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag
     9961 caacatgttt ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg
    10021 ttaagatata ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat
    10081 acacatgcta gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta
    10141 gggcatgaaa atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct
    10201 aagaaaacag gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa
    10261 tctggtcatt tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt
    10321 tctagctcct ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat
    10381 tgagttcgac tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga
    10441 cgatcatttc agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt
    10501 cttctgagat ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct
    10561 cattgaatgg gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga
    10621 tatgagagat cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt
    10681 ctccgccgtc ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt
    10741 ggatcatttt tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca
    10801 tggtgcctcg tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg
    10861 cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg
    10921 ttactaagtt gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat
    10981 cttttcttgt aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg
    11041 ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt
    11101 cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt
    11161 tgacgccttt tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg
    11221 ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt
    11281 attctttcaa agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt
    11341 tcatattatg gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc
    11401 gttcagcttc tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt
    11461 cccagtcacg agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga
    11521 tgtctgaaag gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt
    11581 tcagacggaa ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca
    11641 cttcttctgt caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga
    11701 atttatattc ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga
    11761 tggaaagctg cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc
    11821 gcacatttga tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat
    11881 tggtcgggat ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt
    11941 tctgcttctt ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta
    12001 ctgatggagc agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg
    12061 acttgcaatt cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag
    12121 tcagtcaagc ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat
    12181 aaattgtcta atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc
    12241 aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc
    12301 tgatacttca aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac
    12361 cccttccatg tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc
    12421 aacgactcct gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc
    12481 ttgtaatttt ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat
    12541 accgagtttt tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg
    12601 tgtccctgct tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa
    12661 atcgatcatt tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc
    12721 atataaaaca ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc
    12781 acagtctagt gcttggacat caatggccat gtgcggggca gattgagcac catctactac
    12841 gatgaccccg ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc
    12901 taagacattg gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc
    12961 tgcatcctcc atatccagga agccttcttc tgttagttcg atatatttca agacagcacc
    13021 tgttcgtttt gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag
    13081 cactatctcg tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa
    13141 gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc
    13201 ccgtactttt tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac
    13261 accgcgatga acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg
    13321 ttttggcttt tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc
    13381 ttgaaataag ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc
    13441 ccttcgatta cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca
    13501 gaaccgagga atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc
    13561 atcagatagt acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt
    13621 acttcgtttt catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc
    13681 atcaagacac gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg
    13741 ataccattaa aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg
    13801 atcgtatgag gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag
    13861 ctgattgcca cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag
    13921 tcagcgatca catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt
    13981 aaaatgtgcc cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct
    14041 gaatatttga tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat
    14101 tttgctactt gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca
    14161 tctgccacga tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat
    14221 aaggactcga gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg
    14281 ttcataaaag ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc
    14341 atataatatt cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa
    14401 atgactcctt tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt
    14461 tggacaagca caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg
    14521 atggtttgtg tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata
    14581 tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa
    14641 tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt
    14701 ttttctttca ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct
    14761 ttactgatac cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc
    14821 atgactactc ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat
    14881 cgttgatagt gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct
    14941 ttagcaacga ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca
    15001 aatgtaggtt ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa
    15061 aaaccttcgt tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt
    15121 tcatctaact ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca
    15181 ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc
    15241 atagctaaaa ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc
    15301 ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca
    15361 gataaggtag attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc
    15421 attgttaggt ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt
    15481 atttctaaga cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga
    15541 ctaattgaga atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta
    15601 ttaaacaatt ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta
    15661 cagacttatt tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt
    15721 aacagagccg ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc
    15781 aatcagtttt ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac
    15841 gtaaggagag ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc
    15901 gtaagcgaag tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc
    15961 taaaagtgat gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc
    16021 atcgaatgtt gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg
    16081 tagaattgtg atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc
    16141 atcttttaat tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg
    16201 gacagcaccg gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg
    16261 aatatgctgg aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac
    16321 atagtcatcg aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac
    16381 atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc
    16441 tgctttattt gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt
    16501 cagcaaaagc gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata
    16561 tcctattatc ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat
    16621 acgattagta aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag
    16681 ccttcctgcc aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta
    16741 aatccgatca tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca
    16801 ggtatcaaga ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc
    16861 agtacaccag ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct
    16921 gcggataaaa caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct
    16981 ttagtaaatg gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta
    17041 ctgatgatcg atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata
    17101 gagtaaagga tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat
    17161 gtcatatata gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa
    17221 tctagatatg tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg
    17281 ttctttgatg aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt
    17341 caatgaacca aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc
    17401 gatgttatag cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa
    17461 ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt
    17521 cgaatcagct tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg
    17581 ttgtctgaag acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc
    17641 gactttgttg cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa
    17701 attcaatttc ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc
    17761 caatgcgcta gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc
    17821 gataccgacg cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc
    17881 ttctaacccg actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac
    17941 gccagctaat tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt
    18001 gaaatgctgg aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa
    18061 ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt
    18121 caggagacgg actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc
    18181 ccctttttca acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt
    18241 gaatgtcttt ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta
    18301 tgatttttca aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga
    18361 acttccgtgt taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt
    18421 ggacttagct tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc
    18481 ttacgctcgc ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca
    18541 gcaaacaggg ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt
    18601 ttcatatagg attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag
    18661 tgattttctg attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga
    18721 tccacgcatt catttcatct gaatcattca agatattgat atcttcaccg attttatcat
    18781 taacagaaga aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt
    18841 caacttcgat cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa
    18901 acgtgatgtc tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc
    18961 cattaaataa gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc
    19021 agttctcctt cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag
    19081 atcagtggtc gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc
    19141 gtaaactcgc ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg
    19201 aacaggcgac ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc
    19261 ggtacttctt tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct
    19321 ttggcttttt tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc
    19381 tccttgaaga tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac
    19441 cgatcatgtt ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg
    19501 ttaaaggaag taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca
    19561 tgatcaaacc gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa
    19621 tcatacgata gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga
    19681 agttttcacc gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc
    19741 ttacgttgaa tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg
    19801 gctgataact cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa
    19861 actgataaga tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca
    19921 agaagatcgt tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc
    19981 aagagattcc agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc
    20041 caaagtcatc ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc
    20101 caatcagcca aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta
    20161 cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa
    20221 acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga
    20281 atttgagcaa gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc
    20341 agatccattt ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc
    20401 agacaacttg catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg
    20461 ctacatataa tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat
    20521 aatcaatacg attatcgttc gaagctttta attttttttc catgtttcat cccttttctt
    20581 aaaaaccata ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag
    20641 acatttggga aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag
    20701 cctactgctt atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag
    20761 ctgagattct cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg
    20821 gcaatctcta gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga
    20881 tggaaattca acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag
    20941 cgaccgattc cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc
    21001 ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt
    21061 gcttctctta caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc
    21121 atcaaagagt atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg
    21181 gattcatgtt tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa
    21241 tgcactttag gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca
    21301 aaggtgtggg aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt
    21361 tgctgagttt cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct
    21421 tgcttagttg gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc
    21481 cgctccaact gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct
    21541 gctctggaaa aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg
    21601 gcaccacttc cataactttt tatgataatt actataaaaa ctatttgatt taaatataca
    21661 aaaaaccatc tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag
    21721 atgaagacaa tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt
    21781 attcgttcat cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa
    21841 ctttttgaaa attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg
    21901 aaatctgttt aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat
    21961 gattcttatg atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac
    22021 gttgcatgat ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat
    22081 ttgtacttat tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg
    22141 cgctgttatc ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc
    22201 tgatctttca attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt
    22261 catcactgta actgccggga acttcttccc acttattgga aaaattttct tttggatttc
    22321 attgatctgc tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa
    22381 tatggctcat ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct
    22441 aacaggttat cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc
    22501 tttatctcaa gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg
    22561 tttttatcca agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt
    22621 tactactgtt cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa
    22681 agttgcggaa tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg
    22741 tttcttaatt aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata
    22801 atcgattcaa taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa
    22861 aaacagctgt tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa
    22921 caccgtttac caagaggtcg taa
//
LOCUS       DAFFFQ010000045        22467 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000045
VERSION     DAFFFQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.045"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..640)
                     /locus_tag="LQB69_001764"
     CDS             complement(<1..640)
                     /locus_tag="LQB69_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL3525930.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQ"
     gene            complement(637..1704)
                     /locus_tag="LQB69_001765"
     CDS             complement(637..1704)
                     /locus_tag="LQB69_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL3525931.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(1730..2566)
                     /locus_tag="LQB69_001766"
     CDS             complement(1730..2566)
                     /locus_tag="LQB69_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3525932.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(2563..3432)
                     /locus_tag="LQB69_001767"
     CDS             complement(2563..3432)
                     /locus_tag="LQB69_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3525933.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(3502..4785)
                     /locus_tag="LQB69_001768"
     CDS             complement(3502..4785)
                     /locus_tag="LQB69_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL3525934.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(4799..6484)
                     /locus_tag="LQB69_001769"
     CDS             complement(4799..6484)
                     /locus_tag="LQB69_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL3525935.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            6759..7784
                     /locus_tag="LQB69_001770"
     CDS             6759..7784
                     /locus_tag="LQB69_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3525936.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(8379..12053)
                     /gene="rpoC"
                     /locus_tag="LQB69_001771"
     CDS             complement(8379..12053)
                     /gene="rpoC"
                     /locus_tag="LQB69_001771"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL3525937.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(12111..15728)
                     /gene="rpoB"
                     /locus_tag="LQB69_001772"
     CDS             complement(12111..15728)
                     /gene="rpoB"
                     /locus_tag="LQB69_001772"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL3525938.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            16456..17424
                     /locus_tag="LQB69_001773"
     CDS             16456..17424
                     /locus_tag="LQB69_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3525939.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            17442..18347
                     /locus_tag="LQB69_001774"
     CDS             17442..18347
                     /locus_tag="LQB69_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3525940.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            18344..19150
                     /locus_tag="LQB69_001775"
     CDS             18344..19150
                     /locus_tag="LQB69_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525941.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            19286..20275
                     /locus_tag="LQB69_001776"
     CDS             19286..20275
                     /locus_tag="LQB69_001776"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL3525942.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(20592..21116)
                     /locus_tag="LQB69_001777"
     CDS             complement(20592..21116)
                     /locus_tag="LQB69_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL3525943.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            21403..>22467
                     /gene="scm"
                     /locus_tag="LQB69_001778"
     CDS             21403..>22467
                     /gene="scm"
                     /locus_tag="LQB69_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL3525944.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         6501 a         4796 c         4214 g         6956 t
ORIGIN      
        1 gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt tgcgtagtca
       61 atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg atgatctgca
      121 catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa ttgatcatat
      181 ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct tcggctactt
      241 tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg cttcggggtg
      301 aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata acgattcctt
      361 tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca ccattaagac
      421 gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct gtcactactg
      481 ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt aacaacttac
      541 cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt tctaaaatgt
      601 ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc ctcggacttg
      661 aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt aagcttctgc
      721 gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt aaccgttcat
      781 caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt aatctgggtt
      841 tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg cttcgcgacc
      901 aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga tcccgccata
      961 gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt caataactga
     1021 atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg ctttcatagg
     1081 atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg ctatgggatc
     1141 gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa tttgcccgat
     1201 agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt cccggatacc
     1261 acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat ccaaccgatc
     1321 aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac gaatgggtcc
     1381 ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg tttcttcgat
     1441 cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc gttggcttcc
     1501 ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga taaatggact
     1561 taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa ttactcggat
     1621 acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg ctattcttgg
     1681 ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt tatcctttta
     1741 cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac ataatgatga
     1801 ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag tctggtgtac
     1861 tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac aggaaaatcg
     1921 aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca gaaatgattc
     1981 cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta agaccatcga
     2041 tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt agcatgaata
     2101 tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg gtatcataca
     2161 aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg gaaaacatat
     2221 aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt tttgacaaag
     2281 cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag gagatcacta
     2341 aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata tcctgataat
     2401 gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct tcttgtgaat
     2461 ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct aatagcaaca
     2521 aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca agtccacctt
     2581 ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg atatagctgc
     2641 tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc ctaagatatt
     2701 gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga tagatcgtaa
     2761 aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct tgaacttctg
     2821 ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga ctgtttgcaa
     2881 aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa acaaaacgac
     2941 cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga aaagctgcaa
     3001 cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat atttccaagc
     3061 aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac gcaccgtttt
     3121 cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag ctgtactgcc
     3181 tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa attcactatc
     3241 acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt taggattcag
     3301 cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca ctaaaacagc
     3361 taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag aatgtttcct
     3421 tggacgattc attgaaacct ccccctgata atctgccaat agctatctac ttttttcgaa
     3481 caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta aagcttgatc
     3541 gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac ctgagcttgt
     3601 gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt gtacttcatc
     3661 aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt cattttcttt
     3721 gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag gagacatcaa
     3781 gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt cagcagcctc
     3841 tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc catagacagc
     3901 ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg cttcataaac
     3961 agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca tgaaagcatc
     4021 tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt tttgataaaa
     4081 cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat catccaaaac
     4141 atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg ttccttcagc
     4201 agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt gagcaatttt
     4261 taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt tttctttgtc
     4321 ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt ttccatcttc
     4381 tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt cctcgataac
     4441 gttttggact gccgtctgat caatcagttc atctttatcc atcactttag caaaatcctg
     4501 actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga tttttgtatt
     4561 gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt ctttttcaaa
     4621 atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag agtgcatgaa
     4681 ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag cagtaagaag
     4741 aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt tccacctctt
     4801 attttattat tttcagccac atatattcat acggctggaa tatcaacttt cgttcttgac
     4861 tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt aaaagataac
     4921 ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc gcttcgcgaa
     4981 taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc ggatggaagg
     5041 ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact gctgttctta
     5101 atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc cgattgattt
     5161 ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc agcacacttt
     5221 gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca gctaaaaagc
     5281 gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga tacgtagcgt
     5341 ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact cgggcaccct
     5401 ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata cctcgtaccg
     5461 gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt tttttcgatg
     5521 cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa cgaatcgctt
     5581 gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc tcgccattcc
     5641 caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat accgttcctg
     5701 gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt tctggcaggt
     5761 gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag cgaataaatg
     5821 aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa acttctggat
     5881 ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt ttttctttcc
     5941 cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc acgctgcttg
     6001 tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc aagtttaaat
     6061 attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg aacattaacc
     6121 gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg ttgattgcct
     6181 gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc aagaaatgta
     6241 caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta cgcaaagttg
     6301 gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct tcttcgtccc
     6361 aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt tgttcaatca
     6421 tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa agttttgtat
     6481 acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat attatggaaa
     6541 acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa tacaaataag
     6601 aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc atgatcatgc
     6661 aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt actatggaat
     6721 tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact atttatgaca
     6781 ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat aaccagccaa
     6841 atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg aattatgaac
     6901 cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta gcattatctc
     6961 atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt caatccattt
     7021 ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa acaccagaag
     7081 aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga attattattt
     7141 taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat attcctattg
     7201 tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac acggataatt
     7261 tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt catatcggct
     7321 gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt taccgtcaat
     7381 gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc ggctttcgct
     7441 tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga ccaactgcga
     7501 ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa caatctggaa
     7561 agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg tcctccctat
     7621 tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc aaagccacag
     7681 aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc attctttcaa
     7741 ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga attataaaag
     7801 aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact catccctcgt
     7861 taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta gatagctgtg
     7921 gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa ctatttttaa
     7981 ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc ttatttcaat
     8041 ccatgttatt tggggatact tatctctaaa acataaatag aataacagac atgtctactg
     8101 gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa aataaccgct
     8161 ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact tttgttgatt
     8221 gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg atgttgttaa
     8281 gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca aaaaagaggt
     8341 tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg attgttcatt
     8401 gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt ttcgcttgct
     8461 acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc agctgggatg
     8521 atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt accgcggatc
     8581 gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa gaaactattt
     8641 gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc aggaacgcct
     8701 cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa taatgtacct
     8761 ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat ttgacgaacc
     8821 attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac acgttgtact
     8881 tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa ttgttttgga
     8941 tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc tgctactttc
     9001 atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt gattgttact
     9061 tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt tacttctgtg
     9121 atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg aggaagacct
     9181 tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt caactgagta
     9241 ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac ttcaacgtca
     9301 gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt tgtattacat
     9361 gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg ttttgcgatg
     9421 tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg atgacgtact
     9481 tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat ttcatttccc
     9541 tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc acggatgatt
     9601 acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc agtcttcaag
     9661 gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc caagacggat
     9721 agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt aggagcggcc
     9781 atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg agctccagaa
     9841 tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt ttgttggatg
     9901 ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc atctgtgatc
     9961 agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt ttcaatgatt
    10021 tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc ggcatgtgtc
    10081 gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc tgttactttg
    10141 aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa cggtaatact
    10201 aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa gtatttgtcc
    10261 ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa ttcaggaggc
    10321 atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc tttttgccat
    10381 tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga atgtaaatga
    10441 acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac cattccttcg
    10501 ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat atcttgagaa
    10561 ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc taacatcaac
    10621 atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat ttggtctcca
    10681 tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc gcgtccttgt
    10741 actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc ccggttcagc
    10801 aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc ttcttgacgt
    10861 tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac aagctcacgc
    10921 atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca ttggtacatc
    10981 ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg tttacctagc
    11041 aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga tttcaatggg
    11101 cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa tgcatctact
    11161 gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt caagtctaac
    11221 aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt caagtcactt
    11281 gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg aataacaggg
    11341 ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc ttctaaaata
    11401 tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa ttcttctttc
    11461 aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat cgcttctgca
    11521 cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata ttcgcgttct
    11581 gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac atatgaagca
    11641 aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc catacgtgaa
    11701 ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat atgtcccatg
    11761 cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca aacgattcct
    11821 ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt aggaccaaaa
    11881 atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt aatcgtctca
    11941 ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc caaacctatt
    12001 tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt catttaatgg
    12061 caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga ttagtctaaa
    12121 ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc cacttgttca
    12181 gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt caaagcatca
    12241 acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc tgtttcttcg
    12301 atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa tacgcggaat
    12361 gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc gtatgttttc
    12421 acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt ataagccgca
    12481 ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc aaattgagct
    12541 ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg tgcatgcaat
    12601 ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga cacacgtcca
    12661 tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct ggccataccg
    12721 gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac tggcgttgct
    12781 acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac ttgcccgatg
    12841 ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt tccatcaggt
    12901 aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt tccgtgacga
    12961 ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac acgaactaac
    13021 atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt cacatcgtga
    13081 acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc acgagctttt
    13141 tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac cccttttggc
    13201 gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg gatgataccc
    13261 atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg tgtgatttct
    13321 tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg gatagaagtg
    13381 tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata gttgtaccct
    13441 tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc ttgttccata
    13501 gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg aacgattgga
    13561 cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc gtatttatct
    13621 aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc aacgacaccg
    13681 tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc cataccagta
    13741 ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat gttggcaccc
    13801 atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt cgcaacggca
    13861 actacttgtt taggtgatac gtccatgtag tctactttat caatagatac ttctaagttt
    13921 tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc attcaatggg
    13981 ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta gtcgatttgg
    14041 tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat aaatccgaat
    14101 ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg gccttcagga
    14161 gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac ttcataaccg
    14221 gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt gtgtgtcaat
    14281 tcgccaagag ggtttgtttg atccatgaac tgtgacaact gagaagaacc aaagaattct
    14341 ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa tgtttcagta
    14401 tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc gatacggaat
    14461 tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg gtcgatatca
    14521 tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc aatgatgtcg
    14581 gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac gtttacttca
    14641 cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg atctgtgacc
    14701 acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat gaaaggagct
    14761 agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat ctcgcctgtt
    14821 tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt caaatctaat
    14881 tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg atcgaagaaa
    14941 cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg acgcaaacgt
    15001 tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt atgcaagtct
    15061 ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat cgtatcatcg
    15121 gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt acggtcgata
    15181 cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc acggttaggg
    15241 ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg gaaatagaca
    15301 cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat gatgaatgtt
    15361 ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt gatttcgcca
    15421 gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt tgcatcatgc
    15481 gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac aaattccaaa
    15541 gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc tctaagtccc
    15601 tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg taattccaac
    15661 acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt tactacgtgt
    15721 ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga tcaattgtta
    15781 caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg cttttaggca
    15841 aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt ctacttttgt
    15901 ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt catcagatag
    15961 tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc ctggcgtttc
    16021 ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa ttatctgaca
    16081 attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat tttgtggagg
    16141 aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata acttttactt
    16201 tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa cgacagtttg
    16261 tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac aaaatgctaa
    16321 gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt tcaatttatt
    16381 ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt tcccaaaatt
    16441 aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc cctgctacta
    16501 gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg acaaaaaaca
    16561 attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa aggattcaaa
    16621 gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga ttatcaaaat
    16681 gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa agaagatcct
    16741 gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga aacaaccact
    16801 atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt cacatccaat
    16861 gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga aaaacaaatc
    16921 ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta taacaacggt
    16981 gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa agcgggcgtc
    17041 aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac gaaaagtcta
    17101 gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag tgcggcaagt
    17161 gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc agttgatcaa
    17221 gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg caaacaaaca
    17281 gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc tgtcgaaaca
    17341 gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg catcaatcca
    17401 gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat ttgctctctc
    17461 ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt ggtgtatacg
    17521 tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt cctttaggtg
    17581 cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct tgtcttctag
    17641 gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca aaattgaaaa
    17701 ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc acatcccgtg
    17761 tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat tgggtcgagt
    17821 cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt gctttgatcg
    17881 ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt cgtgcaactg
    17941 gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg aagatcatcg
    18001 gttatatgat ttctaatgga tgcattgctc tttctggctc cttacttgcg caaaacaacg
    18061 gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct tctattatca
    18121 tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact gtcgttttag
    18181 gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt gaaccaagtg
    18241 attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg cagatccagc
    18301 aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg acgtcttaac
    18361 attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc atgtcctgaa
    18421 aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg gaaatggggc
    18481 tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc agggtcaaat
    18541 tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga aatccatcag
    18601 tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag aagaaaatct
    18661 tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca aaccacaaga
    18721 ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa atcgtttatc
    18781 ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat tgatggccac
    18841 attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg accctgctac
    18901 ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc tcactgcttt
    18961 tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga tcatgctgca
    19021 tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca cagtgcccca
    19081 attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc aagtactttt
    19141 atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct tcttttttct
    19201 ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa actattttgt
    19261 tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga caacgaaaga
    19321 caaggtactt caattattga aaaagacacc agattttctt tccggagaaa agttggcaca
    19381 gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg aaaaggcagg
    19441 ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg tactagatgc
    19501 aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac aaacgatgtt
    19561 gacttctgaa tctacgatga aagatgccaa actggcagca gctcaaggag attctgtttc
    19621 gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac ggcctttttt
    19681 cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca aacggtttga
    19741 tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca ttgaatctgt
    19801 aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg gaaaaaaagt
    19861 ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca gtcatatcat
    19921 cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg atctccaaga
    19981 aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc agctaatcag
    20041 caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt tggacttcta
    20101 taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag gaacgactta
    20161 tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa caaatgaaca
    20221 gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc aataattatc
    20281 agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat tctcttcaaa
    20341 agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag gtattctaaa
    20401 aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa caagagagtc
    20461 atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct tatagataga
    20521 ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta agttcttttt
    20581 tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac ctgaccagag
    20641 tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac tggtggtgtc
    20701 cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa gtaagaaata
    20761 gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt agcttggatc
    20821 tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat cgtcatcgga
    20881 aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa atcagcaatc
    20941 tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt gatctgcggg
    21001 gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc aaacaaacgt
    21061 tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata atccatttat
    21121 tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc tacttatatg
    21181 ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag tttggagatt
    21241 gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat gaacgtttca
    21301 ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact aggagaaaaa
    21361 gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa aagggcaagt
    21421 ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc cgtttcagcg
    21481 gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg taactatggc
    21541 ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac tgtgaatgca
    21601 catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt tggtacagag
    21661 cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga ttctttcgct
    21721 aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt tggcagtaac
    21781 gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct tgatcactta
    21841 actccagatg atttttataa agatcgtgct gggcatgaat atattgattt cgagcaggaa
    21901 tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc taaaacatat
    21961 actgctgcag attttccaga catgaacaat cggacaatcg atttaactga tttatcagat
    22021 accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac gccacttcaa
    22081 atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc ttctgcattg
    22141 aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga agatttttcg
    22201 gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat cagtgctcct
    22261 tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa ccaagacgga
    22321 acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga tcctaacgaa
    22381 atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac agatacatct
    22441 accacaagtg atagctcaga ttcatca
//
LOCUS       DAFFFQ010000046        22215 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000046
VERSION     DAFFFQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.046"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            172..516
                     /locus_tag="LQB69_001779"
     CDS             172..516
                     /locus_tag="LQB69_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL3525945.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="LQB69_001780"
     CDS             517..936
                     /locus_tag="LQB69_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBL3525946.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="LQB69_001781"
     CDS             complement(1028..1378)
                     /locus_tag="LQB69_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525947.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="LQB69_001782"
     CDS             complement(1344..2675)
                     /locus_tag="LQB69_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL3525948.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="LQB69_001783"
     CDS             2931..3497
                     /locus_tag="LQB69_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3525949.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="LQB69_001784"
     tRNA            3642..3729
                     /locus_tag="LQB69_001784"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="LQB69_001785"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="LQB69_001785"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL3525950.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="LQB69_001786"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="LQB69_001786"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL3525951.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="LQB69_001787"
     CDS             complement(7452..7898)
                     /locus_tag="LQB69_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL3525952.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="LQB69_001788"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="LQB69_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL3525953.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="LQB69_001789"
     CDS             8363..8881
                     /locus_tag="LQB69_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3525954.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="LQB69_001790"
     CDS             complement(8958..9914)
                     /locus_tag="LQB69_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL3525955.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="LQB69_001791"
     CDS             10080..10886
                     /locus_tag="LQB69_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525956.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="LQB69_001792"
     CDS             10883..11872
                     /locus_tag="LQB69_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL3525957.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQRDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="LQB69_001793"
     CDS             11869..12873
                     /locus_tag="LQB69_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL3525958.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="LQB69_001794"
     CDS             13005..13547
                     /locus_tag="LQB69_001794"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL3525959.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="LQB69_001795"
     CDS             13567..14265
                     /locus_tag="LQB69_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL3525960.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="LQB69_001796"
     CDS             14286..14498
                     /locus_tag="LQB69_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL3525961.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="LQB69_001797"
     CDS             14498..15460
                     /locus_tag="LQB69_001797"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL3525962.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="LQB69_001798"
     CDS             15478..15873
                     /locus_tag="LQB69_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL3525963.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="LQB69_001799"
     CDS             16036..16401
                     /locus_tag="LQB69_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL3525964.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="LQB69_001800"
     CDS             16398..18209
                     /locus_tag="LQB69_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL3525965.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18438)
                     /locus_tag="LQB69_001801"
     CDS             complement(18271..18438)
                     /locus_tag="LQB69_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL3525966.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18686..19675
                     /gene="galE"
                     /locus_tag="LQB69_001802"
     CDS             18686..19675
                     /gene="galE"
                     /locus_tag="LQB69_001802"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL3525967.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19687..21171
                     /gene="galT"
                     /locus_tag="LQB69_001803"
     CDS             19687..21171
                     /gene="galT"
                     /locus_tag="LQB69_001803"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL3525968.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21195..22175
                     /locus_tag="LQB69_001804"
     CDS             21195..22175
                     /locus_tag="LQB69_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3525969.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7208 a         3806 c         4621 g         6580 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggacgggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaagaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattattcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acacacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcaa ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcatc tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt ttagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg attttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaattg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcgggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgcat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaaag
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaat tttttcattg aatccacctc atttattatt tgttatatct attatggaac
    18481 aaaagagaaa aaaaggctag ttttacgttt gtttgtaaac ctttgcaaaa aatccttgaa
    18541 aatgtttact aaatgtttta ttataaattt accatatgaa tttacgaaat tttactttgt
    18601 ttgttttcac cataaaagta aacttcgttt tttaaattat atagaatcaa caaacacaat
    18661 tttactaatc aaaggagtgt tttttatgac aattttagta ctaggcggag ccggttatat
    18721 cggttcgcac gctgtagacc agctagttca aaaggggtat caagtagctg tggtagataa
    18781 tttgctcaca ggtcacaaac aagcagtaca tccagatgct catttttatg aaggagacat
    18841 ccgggataaa gaatttctac gttccgtatt tgaaaaggaa ccgattgaag gagtaatcca
    18901 ttttgctgca agctctttag taggagaatc tgtcgaaaaa ccattgatgt atttcaacaa
    18961 caacgtatac ggtatgcaaa tcttacttga agtcatgcac gagttcaacg tgaataaaat
    19021 cgtattctct tctactgcag caacttatgg agagccgaaa gagtctccaa tcacagaaga
    19081 tacaccagct aatcctaaaa atccgtatgg tgaaagtaaa ttgatgatgg aaaaaatgat
    19141 gaagtggtgt gaccaagcat atggcatgcg ctatgtcgct ttgcgctact tcaatgtagc
    19201 cggagcaaaa gcagatgcat ctatcggaga agatcataca ccagaaacgc atcttgtccc
    19261 aatcatttta caagtagctc ttggtcaaag aaaagccttg gctgtttatg gagacgatta
    19321 tgatacacca gatggaacat gtatcagaga ttatgttcaa gtagaagatt tgattgctgc
    19381 acatatttta gctttggaat acttgaaaga aggaaatgaa agcaacttct tcaatttagg
    19441 cagcagcaaa ggatactcag tcaaggaaat gctagaagca gctagagaag tgacaggaaa
    19501 agaaattcca gcagaaatag caccaagaag agctggagat cctagccgat tagtcgcttc
    19561 aagtgaaaaa gcgagagaga ttctgggctg gaaacctgaa tacacagata tcaaagcgat
    19621 tatcaaaaca gcatgggatt ggcatgtcag ccatccaaat ggttatgaag aataggggaa
    19681 tgagatatgt caatcagtca aacgatttgt gattttgcca cacttgcgat cgaagctggc
    19741 ggatggatgg aaatggaccg cttatattta cagaatcgtc ttttagggat ggtaggagaa
    19801 gatgctctag aaagcattga agtaagacca gttaaggaag aatctgtaga cttattggat
    19861 cagcttgtcg ctaaggcaag agaaaatggt gttctttcag aattgccagc agaacaagag
    19921 atttttgaag cgcaattaat ggattttctc acaccgccac cttcagtcgt caatgcattc
    19981 tttgcacagc actattcgaa aagcccagag gaagcaactg agtatttctt taatttaagt
    20041 aagaaaaatg actacatcaa aacaagagcc atcgcaaaaa acatcaaatt tccggcagag
    20101 tcggagtatg gtgaactgga aatcacaatc aatttatcta agccagaaaa agatccaaaa
    20161 caaatcgcag cagaacgcga ggcggtaaaa gtagactatc ctacctgcat gctttgcatg
    20221 gaaaacgaag gatatagagg tcgcttgaac tatccagccc gcaccaatca ccggattatt
    20281 cgaatgaatc tagacggtga aagctgggga ttccaatatt ccccttatgc ttattacaat
    20341 gaacattcga tcattttatc agaacaacac cgcccgatga aaatcacaaa ggaaacattt
    20401 gctagactgc ttcggattac tgaagtcttg cctcattact ttgttggttc gaatgctgac
    20461 ttaccaattg ttggaggatc gattctttct catgatcatt atcaggcagg acgtcatgaa
    20521 tttcctatgg caaaagcacc gatagaaaaa cttatcaaac tagaagatta tccgcaagtc
    20581 atcgcaggag tcgtgaaatg gccgatgtct gtgatccgtt tgcaatcaac agagaaaaac
    20641 ttgcttattg aagcagctaa ctcagtattg gaaaaatgga aagcatattc ggatgaaaca
    20701 gtatctatca gagctttttc agcagatggt acaccacacc atacgattac gccaattgca
    20761 cgaagaaggg acaattactt tgagttggac cttgtccttc gagacaacaa tgtttcagaa
    20821 gaacatcctg atgggatttt tcatccacat aaagatgtcc aacatatcaa aaaggagaac
    20881 attgggttga tcgaagtaat ggggcttgct gttttaccac ctcgtttgaa accagaacta
    20941 gtagaagtag agcatttctt attaggagaa gaaaaccaga tggctgaata tcatcgtccg
    21001 tgggcagaac aattaaaaaa agaacatcct gatttgacga aagatacggt caccgatgtc
    21061 gtacaaaaat ctgtaggtca ggtttttgca cgtgtactgg aagacgctgg agtattcaag
    21121 cgagatgaaa aaggacaggc agcgtttgaa cgatttactg cgattttata aacgtaaaaa
    21181 aggaggagag acaaatggca acaattaaag acattgcgca gttggcagga gtttctcctg
    21241 ctactgtttc aagagttctc aactatgatc ctgatctttc cgttggctta gccacaaagc
    21301 aaaaaatttt tgaagcagca gaacgcttaa attatacgaa acatcgtaaa aatgcagcaa
    21361 atactaagca aacgcttcgc ctgatccagt ggtacgacga tacggaagag cttgaagatc
    21421 tttattattt atctattcgc ctaggaatcg aaaaaaaggc agaagaagca aacgttactt
    21481 tattgaaaga aacgtgggat tccattagtg atcttccggc agatggaacg atcgcactag
    21541 gaaaattcga cgaagctcag ttattagaaa tgaaaggatc ccaaaatgcc ttgcttttgg
    21601 tagattctga cggcacttct caaggaatcg actctctagt tgtcgacttt aagtcaagtg
    21661 ttcaaaaagt cattgaacac tttgtctccc ataaagcgca acatatcgct atgcttgctg
    21721 gaacagaata tacgaagaaa aaccatcagc ggctaaaaga tcccagaatt atggcatttc
    21781 aacaagtttt ggcagaaaaa ggctacgacg aaggtccaat tatcgaagca gatttttctg
    21841 tcgagtcagg atatcaagca gtgaagcagt atttaaaaac gaatccgcat gttcctgatg
    21901 cgctttttgc agctaatgat gcattagcga ttggcgcact gaaagcgatt caagaaaaag
    21961 gtttagtggt accagaagat atttctgtga tgggcttcaa tgatgtcagt gtagcgaaat
    22021 atgtaacgcc ctcgctttct actgtaaaag tttataccga atggatggga gagctagcag
    22081 tggagacgat tcttcaatta agtaattcca aagcaccggt ttcaagaaag attattgtgg
    22141 aaacagagtt gattattcga gaatcaacga aataaaagat ggaaaagctg tgggaaaatg
    22201 gagtgatacc gcagc
//
LOCUS       DAFFFQ010000047        22130 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000047
VERSION     DAFFFQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.047"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..523
                     /locus_tag="LQB69_001805"
     CDS             <1..523
                     /locus_tag="LQB69_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL3525970.1"
                     /translation="GGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGIVA
                     NCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIFGS
                     LIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILTVL
                     LLPLFFQPFAGLF"
     gene            523..2109
                     /locus_tag="LQB69_001806"
     CDS             523..2109
                     /locus_tag="LQB69_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3525971.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2128..2349
                     /locus_tag="LQB69_001807"
     CDS             2128..2349
                     /locus_tag="LQB69_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL3525972.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2346..3107
                     /locus_tag="LQB69_001808"
     CDS             2346..3107
                     /locus_tag="LQB69_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL3525973.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3487..3996
                     /locus_tag="LQB69_001809"
     CDS             3487..3996
                     /locus_tag="LQB69_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL3525974.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4071..4610)
                     /locus_tag="LQB69_001810"
     CDS             complement(4071..4610)
                     /locus_tag="LQB69_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL3525975.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LQB69_001811"
     CDS             complement(4750..5361)
                     /gene="rpsD"
                     /locus_tag="LQB69_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL3525976.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            5623..6474
                     /locus_tag="LQB69_001812"
     CDS             5623..6474
                     /locus_tag="LQB69_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL3525977.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6502..7137
                     /locus_tag="LQB69_001813"
     CDS             6502..7137
                     /locus_tag="LQB69_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL3525978.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7157..7930
                     /locus_tag="LQB69_001814"
     CDS             7157..7930
                     /locus_tag="LQB69_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3525979.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8338..9135
                     /locus_tag="LQB69_001815"
     CDS             8338..9135
                     /locus_tag="LQB69_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL3525980.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9113..9526
                     /locus_tag="LQB69_001816"
     CDS             9113..9526
                     /locus_tag="LQB69_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL3525981.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9510..12155
                     /gene="mutS"
                     /locus_tag="LQB69_001817"
     CDS             9510..12155
                     /gene="mutS"
                     /locus_tag="LQB69_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL3525982.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12173..14281
                     /gene="mutL"
                     /locus_tag="LQB69_001818"
     CDS             12173..14281
                     /gene="mutL"
                     /locus_tag="LQB69_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL3525983.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14303..14863
                     /gene="maf"
                     /locus_tag="LQB69_001819"
     CDS             14303..14863
                     /gene="maf"
                     /locus_tag="LQB69_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL3525984.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LQB69_001820"
     CDS             complement(14948..16936)
                     /gene="treP"
                     /locus_tag="LQB69_001820"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL3525985.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17159..17872
                     /gene="treR"
                     /locus_tag="LQB69_001821"
     CDS             17159..17872
                     /gene="treR"
                     /locus_tag="LQB69_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL3525986.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            17949..18395
                     /gene="msrB"
                     /locus_tag="LQB69_001822"
     CDS             17949..18395
                     /gene="msrB"
                     /locus_tag="LQB69_001822"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL3525987.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18565..19167
                     /gene="ruvA"
                     /locus_tag="LQB69_001823"
     CDS             18565..19167
                     /gene="ruvA"
                     /locus_tag="LQB69_001823"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL3525988.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19180..20181
                     /gene="ruvB"
                     /locus_tag="LQB69_001824"
     CDS             19180..20181
                     /gene="ruvB"
                     /locus_tag="LQB69_001824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL3525989.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20210..20788
                     /locus_tag="LQB69_001825"
     CDS             20210..20788
                     /locus_tag="LQB69_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3525990.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            20840..21322
                     /locus_tag="LQB69_001826"
     CDS             20840..21322
                     /locus_tag="LQB69_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL3525991.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21439..21813
                     /locus_tag="LQB69_001827"
     CDS             21439..21813
                     /locus_tag="LQB69_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3525992.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7237 a         3991 c         4790 g         6112 t
ORIGIN      
        1 cgggggcaat tttttgacta tgctgattac tcctgcgaca gtggcacttg ccatccctct
       61 ttataaaaac ttccgtttac tgaaagagaa tttttatcct gtaatggctg cgattttagc
      121 aggaatagtc gcaaattgct tggttagtgt agggttcggt tacttcttct ctcttcataa
      181 agagatggtg atctcattac tacctaaatc tgtaacgacg gctatttctg tcgatttatc
      241 tcatacgatg ggcggggtga atgccgtgac acttgctatt gttgtctcta caggtatttt
      301 tggttcgctg atcgccactc atattttccg cctattcaag atcgaaagtc cagttgctcg
      361 cggtgtggcg ttaggaagta cttctcatgc tattggtaca gcaaaagcaa tcgagattgg
      421 tgaaatagaa ggaatcattt ccggacttgc gatatgcgtc aacggaatat tgaccgtttt
      481 attgctaccg ttattctttc agccttttgc tggcttgttt tgatgcgaaa accactattt
      541 tatgctacaa taaccaaaac gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct
      601 tccgagtatc ttaaacaatt tgataaagat ctatattata agattctcct cctagaaagt
      661 tttgaggatc aagcttggca taccgctgca caattagcgc aagttgttca gctggatgcc
      721 cgcagcgtgt cgaaatacct gaacgagctg tctaaaaact atcagcagtt tagcggtaag
      781 acacaccctt tgtttacaaa aaatcaccgt tctggttata acttttatga tacgctcgat
      841 tcaattgaac atgagcgttt tttgatctat ctcgtgcaaa gtacgctgaa attccagttg
      901 ctgcacgaca ttttttttga agaattccat acaatgtacc aatttgcgca aaagcattac
      961 attagtgaat ctactgctca tcgaaaaatc aatgaatgga aacagcaact gcaaacttac
     1021 ggaattcggc tgcaacgagg tacatatatc gctcaaggcg aagaagaaat cattcgactt
     1081 tacttgcata tgacattctg gcagttgttt cgagggaaaa tctggccatt tgaaacgatt
     1141 tctcaaatgg acgtaaagaa tatggcagaa catatcatgg ctttttttaa tgttcgtctg
     1201 aacgagatca aaaaaagacg attggaatat atgctaggtg cctttttttt aagaaaatca
     1261 cagaagcatt atgttgtatt gaatgagaaa aaaagacggc tgatttctga caatctgttg
     1321 ttccagcgat tttgccaggt catggagccg gtatttccta attattttca agtagaagac
     1381 gagttaggcg cattatttct tgtactgatg actcgtgaag aatattatag tgatcctaag
     1441 atacggaaga aaatttttga ttttcatcaa caagcaaaaa caccgccctt tacagccctt
     1501 tcagaagcaa aagccgctct ttctctttat caggaagagc aggggctgcc agcagaaaat
     1561 ctaaccttcg aggcagaaaa ctatttattc agcagtcatt ttttcgccta tctttttcct
     1621 aatgcgaaag aaacgattga tggcaatagt agtgatttta ttaatcattt agttattgaa
     1681 aacaaggaat tgaaacaatg gctcgtacat ttttttgaaa gtagacacaa acatcccaat
     1741 catctggcat ttaaaaatca tgcgtttttg atggggcggt atctgactgt gtttaaaaca
     1801 ttaggagcct ttactccaca gcttccaaag atcacgatcc ttttaatgac agatttccca
     1861 ttatttgaag agcaactgtt agaggaggga ttacggacat ttttccgtaa tgagtatcag
     1921 ctgatttttc tgccaacaga ttatcggggg agagaagttg atcttctgat ttcgacaagt
     1981 aaagtccacc gcaaaccatg ggcggatctt gactatttta ttgtgactga agaactaaaa
     2041 ttaattgatt acatccaact atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta
     2101 aaacaataac aaagaaggag aaaaaagatg acatttgaag aaattttacc agaattgaaa
     2161 aaaggtgcaa agattattcg taaaggatgg agtggatttg aactttatgt tgttctcgta
     2221 tccgatgatg aatttgatgg ctgtccggtg acgccgtact tattgatcaa aacaagtgac
     2281 gaaggttttt ccagcttcgc tcctacagtt tgtgatattt tagcagatga ttgggcgatc
     2341 gtagaatgaa aaaccagtat ccagatctag ctaaacaggt tgtatttatc acaggagctg
     2401 cttctggaat tggtgctgcg caagcccgag cgtttcttga acaagatgct tttgtatttg
     2461 gattagatca aaagttcggt aaaatggaag aaatttcttt gcgatttcct gaaacttttg
     2521 cctacgcact aggtgatgtt cgacaaatga aagaactcca acaagcggta tcgaaatgcc
     2581 aaaatcattt tggtgaagtt acgattttac tgaatacagc ggggatattg gatgcctaca
     2641 aacccttgat ggaaactgac gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa
     2701 gcatgtatca gctgacaaaa atcatccttc ctgatatgat ccgccaaaaa agcggaacga
     2761 tcatcaatat ggcttcgatt gctggcttga ttgctggtgg aggtgggatc gcttatacaa
     2821 gcgcgaaaca tgcgattgtc ggatttacca aacaactcgc tctggattac gcaggacagg
     2881 gtatccgagt aaaaggaatt gctccaggag ccatccaaac accaatgaat gcggctgatt
     2941 ttgaagggaa aggtgagatg gcagagtggg tggcaaatga aacaccagtc aagcgatggg
     3001 cacagccgga agaagtagct gagctgacgt tatttcttgc ctctcctcaa gcaagctaca
     3061 tacaaggaac gatcatccca atcgacggcg gatggttgtt aaaataacct tgtgtttttt
     3121 tcaagcttct gtatgataaa actaaagaga gaaacaacgg ttgatgataa aaaaagtcga
     3181 tgactgttcc cgacattcga tgccgaacag ccagttcaaa aaattctttt taataatcgt
     3241 taatattcga aactaagcgg cttttcacga cctcttgata taaacgatat tcaaaagctt
     3301 gcaactgcgg taacaagtcc agccaaatga aaaatataag gagctatttt tatttgacca
     3361 ttcttattac ttgttgcagg gcagcttttt cactaccttc tgcgacactt cagttggaat
     3421 tttcttccca tcctttggtc gcggtcatta ccaagataaa attatcttgc aaaggagaaa
     3481 tagaacatgg aaaaaaataa tacccgttca gccaaacgct ggacaacgac agatgtgact
     3541 cggatggcgc tagtcactgg tttatatgtg gcagtgacgc tggttttgtc tgtctttagc
     3601 tttggagtga ttcagattcg tctctcggag atgttcaact atctgtcctt gtataacaaa
     3661 agatacatcg tcgcggtaac agcgggagta gctttggcta atattgcttc tcctcttgga
     3721 ctgatagatg ttgcagtagg aagcgtcagc acgctgcttg tattgcttat caactataaa
     3781 atcactagtc gcatcaaaaa catgaaaatc aaaatggccg tgacagcatg cgtatttgct
     3841 ttttccatgt ttacagtagc tggtcagcta acgattttat atcaactacc ttttttcttg
     3901 aattggtggg tgattgcttt aggagaattg gcatcaatga ttgtcggtgg gataattatt
     3961 tattggatcg gtaaaaaagt ggatttgtca aaataaagat ctgtttaatt taaaataggg
     4021 cagcatggaa gagactgaat gttgagtctt cttccatgct gcttttttga ttaatttgta
     4081 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     4141 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     4201 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     4261 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     4321 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     4381 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     4441 ccaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     4501 gtttgccctg tcaaaggaac caaaccaaaa ggaatggtca tctgagaaag gacagaaata
     4561 atcgcggcaa attccgcagc aagtatcaat tctttcaatg ttcggttcat aacgccctct
     4621 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     4681 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     4741 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     4801 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     4861 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     4921 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     4981 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     5041 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     5101 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     5161 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     5221 gtattctgat tgtttgccac ggctattagg tccatgttga cctggtgcgt aagggcgacg
     5281 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     5341 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     5401 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     5461 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     5521 ctgcattatt ttacacaaat tacagtatat gatagatcat ccacgaatgt caagataatt
     5581 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gtatgtgcaa
     5641 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     5701 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     5761 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     5821 gcgatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     5881 atgttcagct ggggtctggt catgattcta tacatgaacg ccgaattagg aacatcgaat
     5941 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     6001 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttctaatt
     6061 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     6121 gtaaaactag caaaaccttc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     6181 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca
     6241 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     6301 cctgccttta cgcttactta ctttgcttct cacggacaga tggatggaat gacagtcagc
     6361 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     6421 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     6481 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     6541 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     6601 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     6661 ttagcttttg ttcattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     6721 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     6781 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     6841 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     6901 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     6961 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     7021 aaagcaattg gttgggcatt acgcgattat agtaagaccg atgccgattg ggtaaaaaga
     7081 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     7141 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     7201 agattcaaac aaacaagctt tagatagcac aagagaagca ttggaaaaat tacgcaaagc
     7261 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     7321 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     7381 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     7441 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     7501 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     7561 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     7621 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     7681 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     7741 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     7801 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     7861 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     7921 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     7981 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagctttcg
     8041 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     8101 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     8161 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     8221 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     8281 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
     8341 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
     8401 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
     8461 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
     8521 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
     8581 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
     8641 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
     8701 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
     8761 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
     8821 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
     8881 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
     8941 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gactgcatta
     9001 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
     9061 ttggatgata cgacaggtca tgcaaaagaa atccagctga ttcagatcaa cgatgatcga
     9121 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
     9181 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
     9241 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
     9301 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaaa aagaagcaat
     9361 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
     9421 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
     9481 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
     9541 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
     9601 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
     9661 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
     9721 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
     9781 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
     9841 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
     9901 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
     9961 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    10021 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    10081 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    10141 ttttgacaag cgaactagtc gatccgctgg aaaaagaagc aaccggcaaa ctattgactt
    10201 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    10261 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    10321 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    10381 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    10441 aagcaagaca agaaatggtc gcttctttgc tagacagcta ttttgaacgt atcgatttgc
    10501 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    10561 atgtcaatgg gcgagacttg atccagctga aaacttcttt agaacaagtg ccaatgatca
    10621 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    10681 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    10741 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    10801 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    10861 gaatcaagaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaattacaa
    10921 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    10981 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    11041 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    11101 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    11161 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    11221 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    11281 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    11341 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    11401 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    11461 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    11521 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    11581 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    11641 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    11701 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    11761 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    11821 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    11881 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    11941 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    12001 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    12061 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    12121 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    12181 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    12241 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    12301 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    12361 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    12421 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    12481 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    12541 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    12601 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    12661 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    12721 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    12781 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    12841 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    12901 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    12961 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    13021 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    13081 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    13141 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    13201 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    13261 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    13321 cgtagaagaa ctaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    13381 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    13441 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    13501 aaagaaagaa tttcccattg tggataacat ggagaaaaca aatgaagaac aagcagataa
    13561 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    13621 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    13681 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    13741 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    13801 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    13861 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    13921 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    13981 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    14041 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    14101 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    14161 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    14221 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    14281 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    14341 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    14401 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    14461 aactgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    14521 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    14581 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    14641 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    14701 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    14761 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    14821 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    14881 ctgattttcg attgaaaatc catgatagcc cgtttttttt attgaaaaga ttacttatct
    14941 actctgacta agattttact tcagctttac cgactgcttc gcccgggcga acaagtcctg
    15001 attggtcaag agaaagtcct gcaacttttt cattattggt caaagcaaca ataatgtcag
    15061 ttttcttgcc agcttgttct atttctggca aatccatttc agcaatcagg gtctctggtg
    15121 tgacaaggta tccttctttc acgaatacat tgaaaggaac accctttagt tcaacagtat
    15181 ctaatcccat gtgaacaagt acttccagcc cctcattaga aagaatacca atggcatgtt
    15241 ttgtttcaaa gacactcgtc actttaccgt ttactggtgc atagactttg ccgtttgacg
    15301 gttcgacagc ataaccgtca cccatcattt tttgtgagaa aactgggtca tttacttcag
    15361 ttatttcctt gatggtccct gcagcgactg cgaataatgt ttcttttgtt gcaactgccg
    15421 taccagttgc tgaaacaggt tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt
    15481 gaagttgtac atcagcagca ttttcaggaa cagcaggatc aatcttattc aaaattccct
    15541 gacgtctaaa gatgatcgtc aagccaaatg gaatgatgac agcaatgatc attgcgatga
    15601 tgaaaggtac ccatgtttct gcttggatag ctagtattcc tggcaaaccg cctactccga
    15661 tcgcgttagc tcgaacaccc atcaagttag caaacatgcc agctaatcca gagccgacca
    15721 ttgcagcgac gaaaggatag acgtatttca aattgattcc gaacatagca ggttcagtta
    15781 cgcctaagta acaagaaatc attgctggaa tcgagatttg ttcttctttt ttattcccac
    15841 gatgtaagaa gatgatggct aagactgcag agccttgggc gatatttgat aaagcaatca
    15901 tcggccataa attcgttgat ccgaaatctg caatcagctg catatcgatg gcattggtca
    15961 tatggtggag accagtaatc actaaaggag catacaggaa tccgaatacg gcactaaaca
    16021 accagctgat agaagacgtc aatccagtat ttacgatatt tgagatccag cttccgactg
    16081 tccagccgat aggacctaaa ataacgtgtg cagctaaaac agttggtaac aatgaaaaca
    16141 atggaacaaa aatcatcgag atcgactgag ggatatattt gcggaagaag atttctagat
    16201 acgctaacat aaaccctgcc agcatggctg gaataacttg cgcttgataa ccaatcatct
    16261 ggacttgagc aaaaccaaaa tcccaaaatg gaatatctgc tgcggctgtg gaagctacac
    16321 tataagcatt taataattgc ggagaaacta acgtaattcc taaaacgatt cctaatattt
    16381 gtgtagtacc catctttttt gcaatactcc aagtgatacc tactggaaga aaatggaaaa
    16441 ttgcttcacc cggtaaccac aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa
    16501 cgattgttcc accaagactt tcaaactgga taccttcaag tacatttctg aatccaagga
    16561 tcaatccacc gacaatgatt gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa
    16621 tagcacgttg aacgggatga aggttttgtt ttgcagctgc ttttccttgt tctttggaaa
    16681 ccccttcaac tccagatact gcaacgaagt cgttataaaa agtagaaaca tcgtttccta
    16741 tgatgacttg gaactgtcct gcattcgtga acatcccttt tacgctaggt atatcttcga
    16801 tcgctttttc gtccgctttt cctggatcat tcaggacaaa acgcatacga gtcgcacaat
    16861 gactaactgc agcaatattt tcttttccgc cgatatcatg taacagcttc tcggcatctg
    16921 cttgatattt tgccatatca ataaacccct ttctcttaac ttgtctatgc aagttgtttc
    16981 ttcatttact atactttttt tcggagagaa ttgcaagcgt tttattaaaa agtacgagag
    17041 aaaatgatat cgttttaaaa agcttaaaat aagcttttct taaaaatagg aacattgaaa
    17101 tacaaaaaga gctgtagtat agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat
    17161 gaataagttc aatgaaattt ttttagattt ggaacagaaa attttagcga aagaatatcc
    17221 tccgcattcg cttttaccca gtgagaacca attgatcaaa atttatggtg tttcacgaga
    17281 aacaattaga aaagcgctga atcttttgac tacggaaggt tatattcaaa aaaaacaagg
    17341 aaaaggttcg atcgtactgg atcggaatcg cttcgacttt cctatttctg gattgactag
    17401 ctataaagag ctacaggaga cccaacgtat tccaagtgaa acgatcatcc atacgttaga
    17461 agaaacagaa gtgacaaaag cattgaacga gatcactggc tgggagatcg gtgcgcctgt
    17521 ttggcatttg atacgcgaaa gaaaaatcaa tggcgaagta gttatattag acaccgatta
    17581 tctattaaag gaaatcgttc ctattttaac tccagcacaa gcacaaggat cgatctatga
    17641 atattttgaa aatgagcttt ctttgacgat tgcttacgcg caaaaggaga tcacagtaga
    17701 agaagtaacg gaaatcatga aaacagcgat ggatcttcac gatgacactt atgcagtagt
    17761 agtcagaagc ctcgttcatc tggaagatac ccgctgtttc gagtattctg agtcggttca
    17821 ccgattagat aaattccgtt tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca
    17881 aaagaaagca aattgttggc atctgtccac tttggcgcta tgataggatc acaaaggggg
    17941 catttttaat ggataaacca ataagaccag aaaaagaaga actgaaagaa aagttatcac
    18001 ccatggctta tgcagtcaca caagaaaatg caacagaacg accatttagc gggaaatatg
    18061 atgattttta cgaaaagggg atctatgtag atatcgtcag cggtgaaccg ttattcagct
    18121 ctgcagaaaa atatgatgca ggttgtggct ggccatcatt cagcaaacca ataacgaaaa
    18181 gacaagtgcg cgaaaaagcc gatttttctc atggcatgca tcgaatagaa gttcgaagca
    18241 aagaagcaga ttctcatctc ggacatgtgt ttaccgatgg accagtagat caaggtggtc
    18301 tacgttactg catcaattct gcctcactga aattcattcc atatgaaaag atggatgaat
    18361 taggttatgg ggaattcaaa tcacttgtag aataatggaa atacacaaag agcggtacaa
    18421 tacggggatt tactcgatcg tccgctcttt ttttattcaa gcgattgcta tttgaaagaa
    18481 acgctcccta gactcctttt ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt
    18541 gtgcaaaaga aagaggacta gaatatgtat gaatatctga caggtgtggt tacctttatc
    18601 aacccctatt acatagtagt agaaaccaat ggtatcggct accagatcgc actaggaaat
    18661 ccttaccgct acagtagcaa actaaatcaa aaaataaaaa tttatgtcca tcaggtaata
    18721 cgtgaagatg cgcatcttct gtatggattt gattcgctag aagaaaagca actgtttttg
    18781 cgattagtaa gtgtttcagg aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa
    18841 gaccactccg gtttgattca agcagtagaa tccagtgatg tcacttattt gacgaaattt
    18901 ccaggagtag ggaaaaagac agcacaacaa atgatcttgg atctaaaagg aaaattcggt
    18961 gagctgagta tcgatacacc ttttagtcta tttgatgaag caaacacaaa agacgctaca
    19021 gctctatctg aagcaatgga agctctctcg gctcttggtt acagtgatcg agaaatcaaa
    19081 cgtgtagaaa aacaattaaa agaagtagat aatcagacaa cggatgagta tctacgccaa
    19141 gcactcaagc taatgatgaa aaaataagga ggagaccaaa tgacagatga atacttgtta
    19201 tcaccagaat ctggcgaaaa tgaactgagt ttggaaaaat ctcttcgacc acagctgctt
    19261 tctcaatata tcggacagag aaaagtcaaa caagaacttc agatttacat cgaagctgcc
    19321 agaaatcggg aagaaccttt agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa
    19381 acgacgatgg ccatggtcat tgctaatgag atgaccgtaa atattcgtac gacgagtggc
    19441 cctgctatcg aacgtccggg tgacttagta gcaatcttga atgaactgga agcaggggat
    19501 gtcctgttta tcgatgagat ccatcgatta cctagagtgg tagaagagat gctttattca
    19561 gctatggaag atttctatgt agatatcatg gtaggacaag ggccaaccgc acatccggtt
    19621 catttccctc ttcctccctt tactttgatt ggcgcgacga cacgggcagg tatgctttct
    19681 gcacctttac gagatcgatt tggcatcatt tctcatatgg aatattatga cgaacaggat
    19741 ctgcaggaga tcgttttacg ttcttcggat atttttcaaa cagagatcat tgaagaaggg
    19801 gcattggaaa ttgctcgccg atcacgagga acgcctagaa tcgctaatcg cttgctcaaa
    19861 cgggttcgtg attttgcgca agtccaagga gacgggaaaa tcgatcgtcc tatagcggat
    19921 caggcactta ctttgctaca ggtcgatcag gctggattgg attatgttga ccagaaattg
    19981 ttgcgaacaa tgatcgagtt atatggcggc ggaccagtag ggttaagtac gctttcagta
    20041 aacataggag aagagcgtga gacggtagaa gatatgtatg aaccgtatct gatccaaaaa
    20101 ggatttttaa agcgtacacc tagagggcga attgctactg cttatgccta tgaacatttt
    20161 ggttacgact atcatatatg atttttgctt ttagattgga ggaaaatgaa tgagtgatac
    20221 aacacgtacg aaacaattga tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt
    20281 tcgaaaaatc agcgtccaaa aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta
    20341 ttatcatttt acagataaat acgatttgct tcgttgggtc tattgtgagg atgcattgca
    20401 ttatttagat ccacaatgtc tttcacttga taattgggaa gagcaagcat tgaaaatgct
    20461 gaaagcaatt gctgagaaga aagaatttta tgcaaatact gtgctttctg acccagaaat
    20521 tttacaaaaa gagttttctc aactgatcca aaaatcattt atcaagattt ttgaacaaat
    20581 ggatgaagaa agacaacttt ctgcaagaga caaactattt tatgctcgtt ttttttctta
    20641 cggttgcagt ggtgtattag tcaattggat cacgaatgaa tatgaagaaa caccgatgga
    20701 aatcgctatg cagcttttcc gtttagccaa ggatacggaa ttatattctt atcgtcttta
    20761 tgcaaaagaa gagttggaag aacagtaata gaaaaatttt ttgttggaaa agcaataatc
    20821 gctaaaagga gatggggaaa tgacaaaaat tttatttgtt tgtttgggaa atatttgccg
    20881 ttcaccgatg gcagaaggct tgctgagaaa acgaatagca ttagaaggaa gggaagcaga
    20941 attttttgtg gattctgctg ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg
    21001 aacgaaaaaa atattaaacc aagaaaaagt agatatgacg aatatggttg ctcggcaaat
    21061 cacaccccac gattttgaaa cttttgattg gatcattggc atggatcaag aaaatgtaga
    21121 agaactgaaa cgaagagcac cacgctcagc gcaggggaaa atccatttgt ttttaagtag
    21181 cattcctgga aaagaaaaag aaaatgttcc cgacccatac tataccaata actttgatga
    21241 gacatatcac ttgatcacag aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa
    21301 taaaataaca aacaatttgt aaagttaacg ctttcttttt aaggcgatgt tagagcattt
    21361 ccttgttttt ttgtaaaatg ttaggtaaaa taaaattaat taagtaacac tttcttagct
    21421 aaaggaggag tgcattcgat gaacgaagag acagtatcgc caatgaagaa gcttacgaag
    21481 ttagttactc atacttcgta tcaacttttt gattcttttg taaatgacac tgaagaacat
    21541 gctaagaatg tacgattgca aggaaagttt attcttgagc tgcaaaaagc tgctactgaa
    21601 catagtctag ttatcttaca agtaagagca aatcgtttga gtagcaagta tgaaacgatc
    21661 tcaggctggg tggcgacaaa aacggtcgaa aaagaccgag tgatgatccg cctacaaaac
    21721 gaagagcagc agatccggat ggttcctgta gaacaaattg aaaaaataac aatcctttca
    21781 ccagcagggg aacaggaacg gttatcaaaa taatttatac cagatagaaa agcacgtatg
    21841 agcttgtacc atcaatcatt actaatcaaa aataaccgaa tgctttaagc tgatatcagt
    21901 aaaacggaac gttttaggat gagtatcata gcatgacagc attcggttga ttagttgaat
    21961 cgattggatg gtacaagttt ttttgataaa aaaaggtcct gctagccggg gagctagcag
    22021 gataaggagt taaaaatgaa aaagtgttgt tagggttgtt tgttggtatg cttatataat
    22081 agtcattagt tgtgaatatt ttgtgacgaa agttttcgaa agttgttaag
//
LOCUS       DAFFFQ010000048        22082 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000048
VERSION     DAFFFQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.048"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            95..1870
                     /locus_tag="LQB69_001828"
     CDS             95..1870
                     /locus_tag="LQB69_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL3525993.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(1936..3522)
                     /locus_tag="LQB69_001829"
     CDS             complement(1936..3522)
                     /locus_tag="LQB69_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL3525994.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            3773..3997
                     /locus_tag="LQB69_001830"
     CDS             3773..3997
                     /locus_tag="LQB69_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL3525995.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            4139..5890
                     /locus_tag="LQB69_001831"
     CDS             4139..5890
                     /locus_tag="LQB69_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3525996.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            5890..7674
                     /locus_tag="LQB69_001832"
     CDS             5890..7674
                     /locus_tag="LQB69_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3525997.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            complement(8023..9363)
                     /locus_tag="LQB69_001833"
     CDS             complement(8023..9363)
                     /locus_tag="LQB69_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3525998.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            9542..10393
                     /locus_tag="LQB69_001834"
     CDS             9542..10393
                     /locus_tag="LQB69_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3525999.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10447..12198
                     /locus_tag="LQB69_001835"
     CDS             10447..12198
                     /locus_tag="LQB69_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526000.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            12202..12372
                     /locus_tag="LQB69_001836"
     CDS             12202..12372
                     /locus_tag="LQB69_001836"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526001.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12412..13314)
                     /locus_tag="LQB69_001837"
     CDS             complement(12412..13314)
                     /locus_tag="LQB69_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3526002.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13545..14948
                     /locus_tag="LQB69_001838"
     CDS             13545..14948
                     /locus_tag="LQB69_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL3526003.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14948..16381
                     /locus_tag="LQB69_001839"
     CDS             14948..16381
                     /locus_tag="LQB69_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL3526004.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16526..17452
                     /gene="trxB"
                     /locus_tag="LQB69_001840"
     CDS             16526..17452
                     /gene="trxB"
                     /locus_tag="LQB69_001840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL3526005.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17666..19393
                     /locus_tag="LQB69_001841"
     CDS             17666..19393
                     /locus_tag="LQB69_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL3526006.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19408..19758
                     /locus_tag="LQB69_001842"
     CDS             19408..19758
                     /locus_tag="LQB69_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3526007.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(19850..20710)
                     /locus_tag="LQB69_001843"
     CDS             complement(19850..20710)
                     /locus_tag="LQB69_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL3526008.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            20802..21230
                     /locus_tag="LQB69_001844"
     CDS             20802..21230
                     /locus_tag="LQB69_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL3526009.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21392..21568
                     /locus_tag="LQB69_001845"
     CDS             21392..21568
                     /locus_tag="LQB69_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL3526010.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21595..22041
                     /locus_tag="LQB69_001846"
     CDS             21595..22041
                     /locus_tag="LQB69_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL3526011.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7094 a         3873 c         4740 g         6375 t
ORIGIN      
        1 tttaaattta gacaaacaag gtatactggt agaaaagtaa aaaaacgaaa agtttcacaa
       61 gcaagtggca aaacgtgatt ggaaggacga aaagatgctg aacactttaa aacccttgat
      121 caaccaacta aaaaagaaac atacatactt tattcttggc atagccctat tatttacaat
      181 tattcgtttg tatttatata gtaaagcaat ctatggaaca gacatgtcgg caacctatga
      241 tgaccagctg ctgattcgct atgctcaaaa tattttggac ggtaaatggc taggggagta
      301 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
      361 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
      421 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
      481 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
      541 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
      601 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
      661 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
      721 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
      781 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
      841 tcctatagtt ggcttttcca tcgtctatca atggatcgaa tataaaaatg agacaacata
      901 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
      961 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     1021 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     1081 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     1141 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     1201 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     1261 caagggaaca atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     1321 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     1381 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     1441 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     1501 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     1561 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     1621 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     1681 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     1741 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     1801 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     1861 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     1921 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     1981 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     2041 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     2101 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     2161 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     2221 gctgttggtc atcatcagta aaggaatcat cgaaaacggc aacgcaaaag ccaagaacac
     2281 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     2341 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     2401 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     2461 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     2521 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     2581 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     2641 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     2701 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     2761 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     2821 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     2881 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     2941 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     3001 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     3061 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     3121 aatatctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ctaatgtctt
     3181 acttgtgcgt gctcggattg cctgtgccaa atccatttgg ctcacgattc ctaattttgc
     3241 tgccatatac tgtaaaagca tagcaatcag actagacatc aaaataactg acatcaataa
     3301 atattggaaa ttttgtcctc cggtgatcga agtagaccag ttacccgggt ccatataccc
     3361 aactgctact aacgcaccag gtccagagta agcaagaagt gttttaaaaa aacttttccc
     3421 ctttggtaca tcaactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     3481 aagtcgatgc ttatgaaatt gtttattgga ttgattattc aagaaaaaac cacctttcct
     3541 atttatctac gattacattc tacacctcaa aaagaaaaaa ttaaagaaaa agattcggta
     3601 cgcctaattt tttttcatgg cgcgtcttgc tagatgacga aagaaatttt gttctcctct
     3661 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     3721 ttagtaaaat aagtcattga gagcaattcg gaaatagaaa ggagagattt atatgttatc
     3781 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     3841 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     3901 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     3961 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaac gactttttac
     4021 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agattattca
     4081 gcaaaaaaga caagcacaga caatcctagg ctcttgataa agacaaagga ggagtaaaat
     4141 gtcgattttt agaaaattag gctggttttt tcgtcaggaa aagaaacatt atttaattgg
     4201 ggttttctcg ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     4261 catcattgat gagatcgccg ataaaaattt atcaataaag atcattttcg ggtggatctt
     4321 cctattgatt atcgctgctt ttttacaata tctttttcgt tatatctggc ggacgaatat
     4381 ttggggaagt gcagctcgat tggagaaaga acttcggcag cagttattca aacattttac
     4441 agaaatggac aatctctttt accaaaaata tcggactggg gatttgatgg ctcacgcaac
     4501 gaatgatttg aatgcgatcc agaatgtagc aggagctggt atcttgacct ttgcagattc
     4561 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     4621 actgattgcg ctgattcctc tgcctttgct ggccgtcact tcacgagtgc tggggtcaaa
     4681 actccatgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     4741 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctagc
     4801 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     4861 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     4921 tttaggtgga aagtatgtct tggaaggcac gatctcgatc gggcagatgg tctcatttat
     4981 tagttatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     5041 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     5101 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     5161 ctcttttaat tatccgaaag aagaatcacc agcattagaa caaatcaagt ttgcgatcca
     5221 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga cgatcatcaa
     5281 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     5341 caaagaatat tcattgaatg ctttattagg atcaatcggc tatgtgcctc aggatcactt
     5401 cttgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     5461 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattaccaaa
     5521 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacaaag
     5581 gatttcgatt gctcgagcat tgatcaaaaa tcctgaatta ttaatattag atgatgcact
     5641 ctctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
     5701 agagaaaaca actatcattg cagctcaccg tttaagtagc gtgatgcatg caaaagaaat
     5761 cattgttgtc gatcaaggga aaatcattga acgaggaacc catcatgagc tgcttgcact
     5821 aggcggctgg tatgaacaaa tgtgggagaa acagcaatta gaagcgaaga tagaaggagg
     5881 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
     5941 cagaaaaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
     6001 gttgctatct tatttgcgtt tgctttgtct gtgatcaatg tcctattgcc aaggatcatc
     6061 caaactttta tggatgatca tctggctaaa cagtcggcta cgacacaagt tattttgttt
     6121 tttgctggta tgtacttatt tggagtcata gtaaaaagta tcatttggtt tttccaatgg
     6181 tatttgtatt ccatggcctc attgaagacc tatcagtata ttagggtaaa attatttgaa
     6241 aagctgcata cactaggtat gcgttatttt gatcagacac cggcaggatc aaccgtttct
     6301 cgagtgacga atgacacaga aacgctattt gaattttggt acgtcttttt gatggtattg
     6361 actgggatct ttgcagtcgt ttcttcattc cttgccatgt ttcaaatcaa tggagaaatt
     6421 gccttgtgga ctttggtgtt tttgccgatt ttaggaatcg tcatctggta ttaccagaaa
     6481 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
     6541 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
     6601 aaagaatttg aacaagtgaa caatgagtat ttagacacgc gttatgcgat gatcaaaacg
     6661 aattcgattc tgttaagtcc gatcatcaat tttctatatg cattggcgat tgcgttggtg
     6721 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
     6781 tttacgacct atgtccaggg attttttaat ccaatgtctc aaatgatgga ctttttgagc
     6841 atcttcacag atgggatggt tgctggtagt cgtatcttga agattatgga tacggaagaa
     6901 tatgcaccac aacaaaaccc tggagcagat caaatgatta cttcaggtaa gattgagttt
     6961 cgcaatgtaa gcttttctta tgatggagag cacaatgtat taaacaatat ctcttttgtt
     7021 gctaatccgg gagagaccgt tgcattagtg ggacacaccg gaagcggaaa aagctcgatc
     7081 atcaacgtat tgatgcggtt ttacgaattt tatgaaggtc agatattgat tgatgatcat
     7141 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tggggctggt cttgcaagat
     7201 gctttcttgt tttatgggga tatcgcagga aatattcgga tgatgaatcc agctatcaca
     7261 gacgaacaga tacgccatgc ggctgagttt gttcaagcaa acaaattcat cgatgaatta
     7321 ccaaaaggat atcactcaaa agtgatcgaa catggtgcca gctattctag tggacaacga
     7381 cagctgatca cttttgcgcg aacaatcgtg actgatccta aaattctagt attggatgaa
     7441 gcaacagcaa atatcgatac tgaaacagaa ggattgatcc aagaaggatt ggcaaaaatg
     7501 cgtcaaggac ggacaacgat tgcgattgcc catcggctgt caacgattcg agatgcagaa
     7561 ttgattttag ttttagacaa aggtcatata gtagaacgcg ggaatcatga aacacttcta
     7621 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtat ataaaagatg
     7681 ttgtgacagt agaattttga cctaagcact agagtgaaaa taaaacatag cttctcatat
     7741 ttttcatctg tgagagttag tgtcgtagtc accagattta aaaaaagatt cgacagctag
     7801 tcagaagtgc ctaatagcca ctaagaaagc gttgacaaaa gcatttgctt tcgataatat
     7861 aaagaaaacg atacaacttg gatagtgatt gcctgatgca agctaaacct gatgagtctc
     7921 ccatggtttc atggacggct ttttgggttt ttctttttgt tggctcttcg tcaatgagtg
     7981 ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag ctatcttgtc
     8041 tagccgtgtt gttgttctgg ctttagtcag ttgggtagtg ttcttagttt tcttgaaata
     8101 ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctaa agccataggc
     8161 aattcgtttt agtactttga tatggttgtt caaacattct atagggccgt tggaataggg
     8221 aactcgcaac gctcgtttga tttctgttcg atatttcttg aaggtcgtga acactggttg
     8281 atagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt caggaacatc
     8341 ttgtcttctg atagcggaaa gaaaaacctg gtagacttcg tagcctcgct ttaaggagtc
     8401 atcataatat aatagccgat cgactatctc cgcttctgtc agatgatctc gaaatgacgg
     8461 acgccagtag cgatgctgac cattcaattc ccacgcattc ttttgaagta gtctccagta
     8521 tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta cttgaattcg
     8581 gtggttggta aaggcccgac cgatgtgttg gctaatatga aagcgatcgg cgacgattcg
     8641 ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat ctgaaaccac
     8701 tagacgtact tgttcacgga cttttctagg gtagcgcaag aaatagtcgc gtaaaggatt
     8761 gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca tcatgacaaa
     8821 gctcatggct cccgagacgt ttttcaccga tttgaattca tcgaaacaca ccacttctgg
     8881 taacggctgg ttcaaagaaa cggttttagg ccgaaggctg cgaagtattc gaatgacagt
     8941 ggtcggagaa atgtgcttca tacgtgcaat caaagacata gaaataggct cacttaatag
     9001 ctcaagaatg gcttgtttga ctctgcgggc aatggagcag cgacgatcgc taactgaatc
     9061 ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt taatccgcac
     9121 aatggtcttg tagttactga catcattcaa taagatggat acttttttcc agccccattt
     9181 aatgatcgtt tgcttatttt gatagtggca atggaagcat tcctctggtt gataggtcaa
     9241 tgtgccatca aaaacaaaac atgtttcgtc attaatgcgc tctttgcgaa aggcatcttc
     9301 atgaaaggta agatttaggt ctagaatatc tagtaactct ttggtaaact ctgaatagga
     9361 catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga tttcaaggtg
     9421 tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt caccaacact
     9481 agatattata gatccttttt gttttggata cagccctata catcatggaa aggagcaaaa
     9541 catgctgatt gagaaagtac tgaataacaa cgtcgtaata accagggatg aaagaaatca
     9601 ggaaatgatc gtgatgggaa aaggtttggc attcaaaaag aaagtgggtg acccgatttt
     9661 tgactcatgc atcgacaaaa aatttcagtt gacaaatgac gggctttcac aacagttcca
     9721 agaattggca caagcgattc ctcttgagta cttagaaatt tcttgtgaaa tcattgaata
     9781 tacaaaagag gctctacaag tccagttgaa tgattctgtg tatattgcat tgacggatca
     9841 tttgtatacg gctatcgaac gtcacaaaat gggaatattt gtaccaaatg tattgctgta
     9901 tgatatcaaa caactatttc ctaaagagta tgccatcggg aaaaaaacag tgaagaaaat
     9961 ctcggataaa taccacatca agttatcaga agatgaagcc ggatttatcg cattgcattt
    10021 agtcaatgca caaggcattc aaagccagca agacaatacc tacctgttta ctataacaat
    10081 acaggatatt ctaaatatcg tccgctatta ctataagatt acatttgatg aagattcagt
    10141 ccattttttt cgattcgtca cacacttacg tttttttctg acacgcgtcc tagatggaga
    10201 aaaatcagaa gtgggagaag tttctgagga attgtttcag gttattcaaa aaaaatataa
    10261 ggatacgttt ggttgcgttg agaaaatagc agacttttta gccgagacgt tcaactacca
    10321 gatgtccacg gacgaaaaaa tgtatttaac gatccatact gcgcgtttag tgcatgagaa
    10381 tcgttgtaaa taaaaaaagg aggataaatt aacaagaacg tatgaaataa gggtagtgac
    10441 attcagttgg ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaataccaa
    10501 gaattagcga gaaaaatcgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca
    10561 cattgtatca caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc
    10621 ttgaaaaata tggacggtat tgtaacggta atgcgagcgg gagggcagta tcaagttgtc
    10681 attgggaatc atgtgccagt cgtgtttgaa gaggttataa aagcaggaaa ccttactttt
    10741 gatgaggctg tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg
    10801 ggctgtttcc agccattcct tgctatccta gctgctggcg ggatgattcg tggactgaca
    10861 gcattccttg tcttcttagg cgcgtttgac cgcggttctg gcacatttgt tatgtttgat
    10921 aatatcggtg attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt
    10981 aaatttaaag taaatgaatt cgtagggatg ttgcttggcg cagcattaat gaatcctagc
    11041 ttgtcgctgg aagctttgag tggtgcagca gaagcaccac taacgaccat attttctggc
    11101 actatttttg aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac
    11161 tacgcttctt ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa
    11221 ccaattaaaa agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt
    11281 cttattacaa tgcctttagg ttttttactc gttggaccag tattcacatt cgcaactgat
    11341 attttaatgg ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc
    11401 gtaggcttct tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg
    11461 ggattgatgc aattttctgt gaatggttgg caaaatatta tgacacctgt tgcagtagtg
    11521 tcttttggac aaactgcagc acttaccgca ctctacttta agcttagaaa tccaaaagac
    11581 aaagcaattg caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt
    11641 tatgggttta cattaccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta
    11701 gggggcgcgt atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc
    11761 tttacaattc ccaactatat tagaccagat ggtgatttaa cagatgtgat taatgtattg
    11821 attgggatcg ccattgcgat ggtggtatcc tttacgctga cttttttctt ttggaaggat
    11881 gaagcaggtg aaacagatga tatacagaaa aaatcaggta aagaaattgt aaaaacaccg
    11941 atacaaggac aaatcgctcc actaaatgca gcaaaggatg cagcttttgc acaaggaaca
    12001 ctaggtcgag gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga
    12061 acagtcatga ccctgttccc gacgaaacag gcaattggaa tggtttctga aacagggctg
    12121 gaattgctga ttcatgtcgg attggatact gtgcagttgg aagggaaata ttttgaaagc
    12181 ttagtgcagc aaggataaag agtgaaacaa ggcgatctgt tgctacgctt cgacaaaaag
    12241 aaaatcgaaa aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat
    12301 tatttggata ttttggaaaa ccaaggggag acggtcgggc cagacgatgc acttatcacc
    12361 gcactgacct gaacggttag gtgaagaatg gactaggctt aactattctt tttattccac
    12421 ttctttattt tcaaaattga aaggctcttt catgatggct gagctgatag ggcgtttatc
    12481 gaatttttga ccgacagaaa ttagatggac taaattctgt tgataatttt ctgcaaaaac
    12541 acaactatat aacacgtcta gaagataaca aattgatgta ttgatcgtaa aattaccaat
    12601 ttttgaatac agtcgctcgc gtgtcgtaag tcgcaaagcg gctgtactca ttgcagatag
    12661 cgttgaatca ccgatactgg tcaaagctaa aattggaaca tgattttgtt tcaatatttc
    12721 tgcggtacga aggatcacat tgttttcacc cgtataagaa atcagaatcc cgcacatatc
    12781 actcgtgcaa ttgtacgctt catagaatga ttcaccaaaa gtggtgacaa tcgtgacatc
    12841 cattttgatg cgcttcattt tcagcatgaa atcttgagaa atcaaagtat tcgagttact
    12901 ggcgaaaatc acgatttttt ttgcttgaag cattagttgt ttggcttttt gcagttggtc
    12961 atgatgaatg agagaaagtg tatcatgaat cgtcatctgt tctaaagaag ccattttatt
    13021 tgcgatcgtc ataataccgt ctgtggtgga aaaaggaaga ttcgcatcta cgtcttgaaa
    13081 attcccttca agataggctg tttcagctaa aaaagcatta cgaaaactga cccagccatc
    13141 aaaacccatt ttttttgcta cacggatgag tgtagagggg tggacataac atcgctcagc
    13201 aatcgcttgg atggtcattt cgccaagtgc tggagaatcg cttaagagaa actggatcac
    13261 ttcattttct gaaggagaaa aggagatttc tttgattttt tcacgtaata acatgtagat
    13321 accttcttct tattttttta cgtaaattat aacttttttg aaaatgattt gtaaacggat
    13381 ttatactata taacttaaaa tagacatttt ttgacaagat atttacaaaa atccactaga
    13441 aaacagtaaa ttttgtaaat gttactggaa agaaacagat ttctattgtt tgttttgttt
    13501 acaaaaaaga taatgaaagt gtaaaaaagt aaaggagtga gaacatggca aaaggaatca
    13561 aaatcgctac gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca
    13621 aaagatatga ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag
    13681 aaaagcttga gatcgttagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag
    13741 tcaagatcta tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga
    13801 ctcagtttcg tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt
    13861 atgggatgat tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg
    13921 ttccagtcgt tttggcaatc gttgaggaca tgaagcgttt atgtccaaat gcttggctga
    13981 tcaatttcgc gaacccaagt gggatggtga cagaagcagt tatcagatat ggcaaatggg
    14041 ataaagtgat tggattatgc aatgtgccag tgatggcgat gatgacagaa ccagcaatgc
    14101 ttggcaagaa aaaagaagag ctgatctaca agtttgccgg tctaaatcat ttccattggc
    14161 acaaagtagc agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg
    14221 atgaagagaa tggattaccg aaaaatatct ttgatatccc gtttttcaaa gaacaattaa
    14281 aacaaatgaa catgatccct tgtggctacc accgctatta ctatcgtgaa gaagaaatgc
    14341 tggaacatgc tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa
    14401 cagaagcaga gctatttgaa ttatacaaag atccagaatt agaccataaa cctgaacaat
    14461 tgcaaaaacg aggtggcgct tattattcag acgctgcctg tgaaacgatt gcttcaatct
    14521 atggaaataa agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgacc
    14581 tgccggcaga ttgtgtagta gaagtcactg cctatgttgg cgcacaagga gcgagaaatg
    14641 tagcgtttgg ttctcttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg
    14701 aattgctgac gatcgaagca gctgtgactg gtgattatgg gacagcttta caggcgttta
    14761 cgatcaatcc acttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta
    14821 ttgcacacca gcagtattta ccgcaattca aggagacaat cagccgatta aaagaagaag
    14881 gaatcgaagt tcaagatgaa ctggcaagag aattatcagg taatacacat ataatggagg
    14941 aagtataatg ggattcagaa aagatttttt atggggcggt gctacagcag ccaatcaatg
    15001 tgagggcggt tatgatgaag gtggacgcgg actagcaaac gtagatgttg tgccaatcgg
    15061 aaaagaccgt tttcctgtta tcaccggaaa gaaaaaaatg tttgaatttg atgaggatca
    15121 tttttatcca gcaaaaattg caatcgatat gtatcatcgt tataaagaag atattgcttt
    15181 gtttgcagaa atggggttca agacttatcg aatgtccatc gcctggagtc gtatttttcc
    15241 aatgggggat gagaaagagc cgaatgaagc tggcttgaaa ttttatgagg atgcctttaa
    15301 agaatgccaa aagcacggaa ttgagccact tgtgacgatt acgcattttg attgtccaat
    15361 gcatttgatc aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa
    15421 tctgtgtcga gtcatcttca atcgctataa aggacttgtc aaatattggc tgacattcaa
    15481 tgagatcaat atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaaaaagg
    15541 agaaaatgaa gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat
    15601 cgcaacgaag attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc
    15661 aggagcatat tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga
    15721 tcgagaaaat tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt
    15781 gaaagaaatg gagcgaaatg gcattcagat cgaaatggaa ccaggggatg ccgcattgct
    15841 gaaagaacat actgttgatt ttatctcttt ttcttattat gcttcccgag tttcgactac
    15901 cgacaacgaa ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata
    15961 tctgcaagca agtgaatggg ggtggcaaat tgatccatta ggcttgagaa taacgatgaa
    16021 tgatatttat gatcgttatc aaaaaccatt gtttattgta gaaaacggct taggtgcagt
    16081 cgatacgcca gatgaaaatg gttatgtgga agatgattac cgaatcgatt acctggcagc
    16141 gcatatccag gcgatgaagg atgcagtaga actagacgga gtcgatctat taggctatac
    16201 gacttgggga tgtattgatt tagtatcagc tggtaccggt gagatgaaga aaaggtacgg
    16261 attcatttat gtcgatcgtg ataaccaagg aaatggcaca ttagcccgtt caaagaaaaa
    16321 atcattcaac tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaacgattg
    16381 atcaatgatt tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat
    16441 tcgagaaacg caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact
    16501 tgattaagta aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc
    16561 tgggatgact gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca
    16621 aggtgcccct ggcggccaaa tgaacaatac ggcagaaatc gaaaattatc ctggattcga
    16681 ttcgatcatg ggccccgatc tagcttataa gatgtatgaa agtgtctcaa gatttgggac
    16741 agaaaatgct tatggaatcg ttcaagatat caaggatcac ggcagccata aagaagtcat
    16801 ctgtgaagat aaaagttacg aaggaaaaac agtcattatc gcaacaggat gtatccaccg
    16861 taaattaggc gtacctggcg aggaagaatt tgctggacga ggcgtttctt actgtgcagt
    16921 ctgtgatggt gcgtttttcc gtaacaaacg tctagtagtc atcgggggtg gggactcagc
    16981 agtagaagaa gccatctatt tgacacaatt tgcttctgaa gtagtgatcg tccaccgtag
    17041 agatgaatta cgtgcacaaa agatcatcca agaccgtgca tttgcaaatg aaaaaatttc
    17101 ctttatgtgg gatacagtcg ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt
    17161 gaaagcacgt aatgtcaaaa ccggcaatga atttgattta gcagcagacg gagtattcat
    17221 ctatgttgga ctggatccat taactgcacc ttttagagaa gcaggtttga cgaacgaagc
    17281 tggctggatt ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg
    17341 agatgttcgt gaaaaagatt tgcgacaaat cactacagct gttggtgacg gcggtattgc
    17401 tggacaacaa gtgtataaat acttggagtc tttagaagaa acaacagcat aagtttaaaa
    17461 agagtagaag aacgggttca attgttcttt tactcttttt tttcgcgtga aatttactga
    17521 atctgattta tgcgaacacg tccaacacct tttgataaaa gaatcctttc ttgatttaag
    17581 cgttttttgg tcctcatact ttcatatcta ataagtgaat gctatactta ttaagaacca
    17641 tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca
    17701 tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga
    17761 aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt actgcaggta tgcggggaat
    17821 cttaggacct gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact
    17881 agcacgtttc atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta
    17941 tgactcacgc catcagtcac cagaatttgc aatggaagca gccaaaacac tagcacatca
    18001 caatatcccg tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt
    18061 tcgtcatttg aagacgtttg cgggaatcat ggtaaccgca tctcacaacc cagcggctta
    18121 caatggatat aaagtctacg gagaagatgg cggacaaatg cctcctgcag acgcagatgc
    18181 attgacgaaa tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga
    18241 agaagctaaa catagcgggt tgatcacgat catcggagaa gaagtggacg ctgcttatct
    18301 agaagaaatc aaagcagtga cgattgatcg tgaattggtt gaaacaatgg ggaaagattt
    18361 gaaacttgtg tacacgccac ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa
    18421 acaagctgga ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag atccagattt
    18481 cactacggtc aaatcgccaa atccagaaga acattcagct tttgaatatg ctatacgact
    18541 tggagaaaaa gaaggagctg acttgctgat tgcaacagat cctgatgcag accgtttagg
    18601 cgcagcggtt cgtttgccag atggttctta tgaagtattg actgggaacc aaatcggagc
    18661 attgtttgct caatatattt tggaagcaca tcaatcaaga ggcactttac cagaaaacgc
    18721 agcggtattg aaatcaatcg tttccagcga attgcctaca gcaattgcgg aacactacgg
    18781 tgctaccatg ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa tccaacaatt
    18841 tgaagaagac cattcacata cttttatgtt tggattcgaa gaaagttatg gttatttagt
    18901 taaacctttt gttcgcgata aagacgcgat ccaagcattg cttttattag cagaagtagc
    18961 agcttactat aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac
    19021 ctatggctat ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc
    19081 gaaaatctct gctttgatgg aaaaattccg taaagaagca ccgacagaat ttgccggagc
    19141 aaaagttatt caaacagaag actttaaaga attgacgaga acagaccgtg aaggaaacgt
    19201 agaaaaacta acaacaccgc catccgacgt attgaagtat acattggaag aaaatggctg
    19261 gatcgctatc cgcccttcag gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg
    19321 aagctctaca gaagacgcag ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat
    19381 gacagaagaa taaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct
    19441 tttttcaagt tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt agagaaagtc
    19501 agtcaattat tcaaaatgtt gagtgatccc actcgtctta aaattctgct atatctgaaa
    19561 gacggggaac aaaatgtgac agcaatcacc caagcagtgg aaatggagca gtctgctgta
    19621 tcgcatcaac tacgtttatt aagagaaaat catgtcgtca agtctcatcg cgagggaaaa
    19681 gcgattttgt atagtcttga tgattctcat gtactggata ttttgaacca gacactcaaa
    19741 catgtggaac atcaataacg ataggtgaaa atagataaac actggagtgt gacaacgaag
    19801 aaatttcgtt gtcacactcc agtgtttttt aagaaaaaga caaaaatgat taatcgcttt
    19861 tttctgttgt ttcagttcca taataactat gatcttctag gtctaaagca ttcaaaataa
    19921 gcgaagccgt ttcttcaata gataaagatg ccacattgat cactacacat cctagttttt
    19981 cataaagatc attcgcaaaa tcgagttcag ctctaatttt ttcgatatcg gaataagcag
    20041 tatcagggtt taatccgtaa gagcgcatcc gttctttacg gatattattt aaaatatctg
    20101 ggttattcgt tagtcccacg atttttttag gatcgatttc ccacagctgt ttgggaatat
    20161 gtgcttgggg aatcaaaggt aaattagcaa cttttaaatt tttatttgct aaaaacaagc
    20221 tcaaaggcgt tttggaagtt cgggatacgc ctagcaaaac gacatctgct tctaaaaaac
    20281 cacgaggatc tttcccatca tcgtatttta cagcaaattc cattgctttg atccgtttga
    20341 aatagttctc attcaataaa tggagggcac caggttgccg aattggcttg ataccagtta
    20401 aacgttcaat ttctgatatc ggtggcgtca tcacatcgaa actaaataat tgatgttctt
    20461 cacaaaaaga acgtgttatc tcgatcagtt cttgattgat taatgtatgc aggacaagtg
    20521 ctttttcttc taatgcatct tgcaatgcct gccttaatgt tttttcatct gtcacgaacg
    20581 ttctgcggaa taggttgaac tcaacggtag gatactgagc catagtagct tgcgctagtt
    20641 ttgtcgcagt ttctccagca gagtcggaga tgacaaaaaa agtaacgatt tctgtagaat
    20701 cggaagacat ggataaaacc tcacttgcat tttttttatg tttattatac catactctag
    20761 aatgcgtact tcgtaatcat tttgatttgg aggaataagt catgaaaaaa cagacattga
    20821 tcgaacaatc attagaaaca ttgaaaaata acggttttaa atatactaaa aaaagagaag
    20881 cattgttaac ttatttagtg aagcgtaacc gctacgtatc tgctaaagaa gtttttgact
    20941 atatgaatca ggaattcaaa ggggtaagtt atgatacgat ttaccggaat ctccatgatt
    21001 ttgcagagat cgatttactg gaagaaaccg atttaaatgg tgaaatgaag tttcgttttc
    21061 attgctgtca gggggaaatc ggccatcacc atcattttat ctgtacagtg tgcggaacaa
    21121 cgaaagaaat ccatatgtgt ccaatgaatt atttcgaaga acagctaggt ggatgcacga
    21181 tcgaagggca tagattcgag attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat
    21241 aaaataacat ttgataattt aaaaaaaaag caagatttcc ttgacggtca aaacagcatt
    21301 agatataata tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt
    21361 tactttttaa gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga
    21421 atcacttgac gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca
    21481 agaatctcgt aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga
    21541 agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca
    21601 ctactaagta gattaaacga tgacatgaaa gcagcgatga aagcgaaaga taaggaatct
    21661 ttacaagtca tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac
    21721 gacttgacag aagaagaaga actaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat
    21781 tctttgcatg agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa
    21841 attgtaatag tcgaaaaata tatgccggaa caactttccg acgaagaaat tcgacaactt
    21901 gttcaagaag caatcactca aactggtgct tcatcaatga aagaatttgg caaagttatg
    21961 ggtgcaatta tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg
    22021 aaagaactat tacaatctta attttatcat ttaaactgcg gacagtgatt gtccgcagtt
    22081 tt
//
LOCUS       DAFFFQ010000049        20778 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000049
VERSION     DAFFFQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.049"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(18..1574)
                     /gene="rny"
                     /locus_tag="LQB69_001847"
     CDS             complement(18..1574)
                     /gene="rny"
                     /locus_tag="LQB69_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL3526012.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LQB69_001848"
     CDS             complement(1985..3034)
                     /gene="recA"
                     /locus_tag="LQB69_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL3526013.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3143..4384)
                     /locus_tag="LQB69_001849"
     CDS             complement(3143..4384)
                     /locus_tag="LQB69_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL3526014.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LQB69_001850"
     CDS             complement(4624..5202)
                     /gene="pgsA"
                     /locus_tag="LQB69_001850"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL3526015.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5411..6334)
                     /locus_tag="LQB69_001851"
     CDS             complement(5411..6334)
                     /locus_tag="LQB69_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526016.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7073..8359)
                     /locus_tag="LQB69_001852"
     CDS             complement(7073..8359)
                     /locus_tag="LQB69_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL3526017.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8352..9620)
                     /locus_tag="LQB69_001853"
     CDS             complement(8352..9620)
                     /locus_tag="LQB69_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL3526018.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9872..10333
                     /gene="mscL"
                     /locus_tag="LQB69_001854"
     CDS             9872..10333
                     /gene="mscL"
                     /locus_tag="LQB69_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL3526019.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10392..10838)
                     /locus_tag="LQB69_001855"
     CDS             complement(10392..10838)
                     /locus_tag="LQB69_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3526020.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10931..11176)
                     /locus_tag="LQB69_001856"
     CDS             complement(10931..11176)
                     /locus_tag="LQB69_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL3526021.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11290..11868)
                     /locus_tag="LQB69_001857"
     CDS             complement(11290..11868)
                     /locus_tag="LQB69_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3526022.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11945..12475)
                     /locus_tag="LQB69_001858"
     CDS             complement(11945..12475)
                     /locus_tag="LQB69_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL3526023.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LQB69_001859"
     CDS             complement(12479..13339)
                     /gene="rlmB"
                     /locus_tag="LQB69_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL3526024.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13332..13727)
                     /locus_tag="LQB69_001860"
     CDS             complement(13332..13727)
                     /locus_tag="LQB69_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL3526025.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13724..15136)
                     /locus_tag="LQB69_001861"
     CDS             complement(13724..15136)
                     /locus_tag="LQB69_001861"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL3526026.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LQB69_001862"
     CDS             complement(15133..15669)
                     /gene="cysE"
                     /locus_tag="LQB69_001862"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL3526027.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15755..15994)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15991..17451)
                     /locus_tag="LQB69_001863"
     CDS             complement(15991..17451)
                     /locus_tag="LQB69_001863"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL3526028.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17621..18778)
                     /locus_tag="LQB69_001864"
     CDS             complement(17621..18778)
                     /locus_tag="LQB69_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL3526029.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LQB69_001865"
     CDS             complement(18812..20182)
                     /gene="radA"
                     /locus_tag="LQB69_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL3526030.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20245..20772)
                     /locus_tag="LQB69_001866"
     CDS             complement(20245..20772)
                     /locus_tag="LQB69_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL3526031.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         5473 a         4590 c         3706 g         7009 t
ORIGIN      
        1 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
       61 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
      121 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
      181 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
      241 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
      301 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
      361 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
      421 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
      481 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
      541 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
      601 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
      661 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
      721 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
      781 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
      841 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
      901 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
      961 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
     1021 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
     1081 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
     1141 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
     1201 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
     1261 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
     1321 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
     1381 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
     1441 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
     1501 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
     1561 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
     1621 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
     1681 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
     1741 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
     1801 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
     1861 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
     1921 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
     1981 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
     2041 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
     2101 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
     2161 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
     2221 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
     2281 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
     2341 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
     2401 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
     2461 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
     2521 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
     2581 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
     2641 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
     2701 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
     2761 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
     2821 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
     2881 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
     2941 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
     3001 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
     3061 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
     3121 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
     3181 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
     3241 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
     3301 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
     3361 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
     3421 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
     3481 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
     3541 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
     3601 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
     3661 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
     3721 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
     3781 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
     3841 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
     3901 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
     3961 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
     4021 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
     4081 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
     4141 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
     4201 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
     4261 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
     4321 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
     4381 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
     4441 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
     4501 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
     4561 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
     4621 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
     4681 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
     4741 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
     4801 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
     4861 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
     4921 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
     4981 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
     5041 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
     5101 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
     5161 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
     5221 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
     5281 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
     5341 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
     5401 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
     5461 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
     5521 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
     5581 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
     5641 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
     5701 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
     5761 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
     5821 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
     5881 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
     5941 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
     6001 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
     6061 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
     6121 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
     6181 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
     6241 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
     6301 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
     6361 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
     6421 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
     6481 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
     6541 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
     6601 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
     6661 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
     6721 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
     6781 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
     6841 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
     6901 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
     6961 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
     7021 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
     7081 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
     7141 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
     7201 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
     7261 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
     7321 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
     7381 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
     7441 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
     7501 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
     7561 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
     7621 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
     7681 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
     7741 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
     7801 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
     7861 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
     7921 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
     7981 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
     8041 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
     8101 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
     8161 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
     8221 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
     8281 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
     8341 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
     8401 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
     8461 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
     8521 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
     8581 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
     8641 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
     8701 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
     8761 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
     8821 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
     8881 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
     8941 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
     9001 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
     9061 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
     9121 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
     9181 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
     9241 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
     9301 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
     9361 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
     9421 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
     9481 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
     9541 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
     9601 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
     9661 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
     9721 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
     9781 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
     9841 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
     9901 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
     9961 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    10021 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    10081 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    10141 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    10201 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    10261 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    10321 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    10381 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    10441 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    10501 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    10561 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    10621 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    10681 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    10741 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    10801 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    10861 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    10921 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    10981 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    11041 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    11101 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    11161 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    11221 ttcaagttta ccataaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    11281 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    11341 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    11401 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    11461 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    11521 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    11581 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    11641 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    11701 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    11761 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    11821 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    11881 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    11941 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    12001 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    12061 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    12121 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    12181 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    12241 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    12301 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    12361 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    12421 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    12481 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    12541 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    12601 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    12661 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    12721 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    12781 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    12841 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    12901 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    12961 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    13021 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    13081 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    13141 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    13201 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    13261 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    13321 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    13381 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    13441 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    13501 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    13561 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    13621 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    13681 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    13741 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    13801 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    13861 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    13921 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    13981 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    14041 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    14101 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    14161 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    14221 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    14281 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    14341 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    14401 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    14461 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    14521 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    14581 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    14641 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    14701 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    14761 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    14821 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    14881 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    14941 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    15001 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    15061 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    15121 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    15181 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    15241 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    15301 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    15361 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    15421 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    15481 agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata
    15541 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    15601 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    15661 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    15721 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    15781 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    15841 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    15901 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    15961 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    16021 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    16081 tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg
    16141 aacagctttg atggcagctt tgacagttgg agcatctact acttccattt gttctagctg
    16201 tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    16261 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    16321 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    16381 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    16441 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    16501 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    16561 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    16621 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    16681 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    16741 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    16801 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    16861 attataagtt ggcataccat cgcgtttttg tatgacaaag tcgccaccga tattatcgga
    16921 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    16981 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    17041 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    17101 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    17161 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    17221 tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg
    17281 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    17341 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    17401 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    17461 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    17521 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    17581 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    17641 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    17701 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    17761 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt
    17821 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    17881 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    17941 agactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    18001 tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    18061 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    18121 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    18181 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    18241 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    18301 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    18361 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    18421 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    18481 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    18541 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    18601 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    18661 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    18721 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    18781 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    18841 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    18901 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    18961 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    19021 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    19081 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    19141 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    19201 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    19261 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    19321 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    19381 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    19441 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    19501 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    19561 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    19621 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    19681 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    19741 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    19801 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    19861 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    19921 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    19981 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    20041 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    20101 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    20161 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    20221 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    20281 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    20341 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    20401 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    20461 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    20521 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    20581 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    20641 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    20701 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    20761 tctttttctc accggttt
//
LOCUS       DAFFFQ010000050        20663 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.050, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000050
VERSION     DAFFFQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.050"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            168..362
                     /locus_tag="LQB69_001867"
     CDS             168..362
                     /locus_tag="LQB69_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526032.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..929
                     /locus_tag="LQB69_001868"
                     /pseudo
     CDS             631..929
                     /locus_tag="LQB69_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1026..1460)
                     /locus_tag="LQB69_001869"
     CDS             complement(1026..1460)
                     /locus_tag="LQB69_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3526033.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="LQB69_001870"
     CDS             complement(1692..2402)
                     /locus_tag="LQB69_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL3526034.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="LQB69_001871"
     CDS             complement(2392..3252)
                     /locus_tag="LQB69_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL3526035.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="LQB69_001872"
     CDS             complement(3257..3895)
                     /locus_tag="LQB69_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL3526036.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="LQB69_001873"
     CDS             complement(3910..4209)
                     /locus_tag="LQB69_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3526037.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="LQB69_001874"
     CDS             complement(4252..5736)
                     /locus_tag="LQB69_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL3526038.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="LQB69_001875"
     CDS             complement(5756..6217)
                     /locus_tag="LQB69_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526039.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LQB69_001876"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="LQB69_001876"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL3526040.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="LQB69_001877"
     CDS             7520..8290
                     /locus_tag="LQB69_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3526041.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="LQB69_001878"
     CDS             complement(8563..9390)
                     /locus_tag="LQB69_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526042.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="LQB69_001879"
     CDS             9958..11193
                     /locus_tag="LQB69_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL3526043.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="LQB69_001880"
     CDS             complement(12192..12452)
                     /locus_tag="LQB69_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL3526044.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="LQB69_001881"
     CDS             complement(12479..13780)
                     /locus_tag="LQB69_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526045.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="LQB69_001882"
     CDS             complement(14072..14530)
                     /locus_tag="LQB69_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526046.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15874)
                     /locus_tag="LQB69_001883"
     CDS             complement(14543..15874)
                     /locus_tag="LQB69_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL3526047.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGRDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17327)
                     /locus_tag="LQB69_001884"
     CDS             complement(15885..17327)
                     /locus_tag="LQB69_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526048.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="LQB69_001885"
     CDS             complement(17314..18078)
                     /locus_tag="LQB69_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL3526049.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="LQB69_001886"
     CDS             complement(18182..19057)
                     /locus_tag="LQB69_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3526050.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="LQB69_001887"
     tRNA            complement(19312..19396)
                     /locus_tag="LQB69_001887"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="LQB69_001888"
     tRNA            complement(19414..19485)
                     /locus_tag="LQB69_001888"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="LQB69_001889"
     tRNA            complement(19495..19567)
                     /locus_tag="LQB69_001889"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="LQB69_001890"
     tRNA            complement(19629..19713)
                     /locus_tag="LQB69_001890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="LQB69_001891"
     tRNA            complement(19734..19804)
                     /locus_tag="LQB69_001891"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="LQB69_001892"
     tRNA            complement(19850..19921)
                     /locus_tag="LQB69_001892"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="LQB69_001893"
     tRNA            complement(19933..20005)
                     /locus_tag="LQB69_001893"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="LQB69_001894"
     tRNA            complement(20008..20081)
                     /locus_tag="LQB69_001894"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="LQB69_001895"
     tRNA            complement(20088..20168)
                     /locus_tag="LQB69_001895"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="LQB69_001896"
     tRNA            complement(20170..20245)
                     /locus_tag="LQB69_001896"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="LQB69_001897"
     tRNA            complement(20281..20353)
                     /locus_tag="LQB69_001897"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="LQB69_001898"
     tRNA            complement(20363..20435)
                     /locus_tag="LQB69_001898"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="LQB69_001899"
     tRNA            complement(20451..20522)
                     /locus_tag="LQB69_001899"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="LQB69_001900"
     tRNA            complement(20537..20626)
                     /locus_tag="LQB69_001900"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
BASE COUNT         6529 a         4320 c         3276 g         6538 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgtccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atgcttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcgtcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaaa ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aacggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gag
//
LOCUS       DAFFFQ010000051        20383 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000051
VERSION     DAFFFQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.051"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(546..1433)
                     /locus_tag="LQB69_001901"
     CDS             complement(546..1433)
                     /locus_tag="LQB69_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL3526051.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            1618..2457
                     /locus_tag="LQB69_001902"
     CDS             1618..2457
                     /locus_tag="LQB69_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3526052.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2653..3627
                     /gene="sgrA"
                     /locus_tag="LQB69_001903"
     CDS             2653..3627
                     /gene="sgrA"
                     /locus_tag="LQB69_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL3526053.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            3925..4281
                     /locus_tag="LQB69_001904"
     CDS             3925..4281
                     /locus_tag="LQB69_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526054.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            4284..5441
                     /locus_tag="LQB69_001905"
     CDS             4284..5441
                     /locus_tag="LQB69_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526055.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5467..5907)
                     /locus_tag="LQB69_001906"
     CDS             complement(5467..5907)
                     /locus_tag="LQB69_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL3526056.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            6067..6573
                     /locus_tag="LQB69_001907"
     CDS             6067..6573
                     /locus_tag="LQB69_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3526057.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            6741..8654
                     /locus_tag="LQB69_001908"
     CDS             6741..8654
                     /locus_tag="LQB69_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL3526058.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVEKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            8659..9660
                     /locus_tag="LQB69_001909"
     CDS             8659..9660
                     /locus_tag="LQB69_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3526059.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(9862..10068)
                     /locus_tag="LQB69_001910"
     CDS             complement(9862..10068)
                     /locus_tag="LQB69_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL3526060.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(10714..11562)
                     /locus_tag="LQB69_001911"
     CDS             complement(10714..11562)
                     /locus_tag="LQB69_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3526061.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            11561..12628
                     /gene="gnd"
                     /locus_tag="LQB69_001912"
     CDS             11561..12628
                     /gene="gnd"
                     /locus_tag="LQB69_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL3526062.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            12630..13631
                     /locus_tag="LQB69_001913"
     CDS             12630..13631
                     /locus_tag="LQB69_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL3526063.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            13628..13912
                     /locus_tag="LQB69_001914"
     CDS             13628..13912
                     /locus_tag="LQB69_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL3526064.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            13945..14742
                     /locus_tag="LQB69_001915"
     CDS             13945..14742
                     /locus_tag="LQB69_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3526065.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            14756..15589
                     /locus_tag="LQB69_001916"
     CDS             14756..15589
                     /locus_tag="LQB69_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526066.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            15607..16464
                     /locus_tag="LQB69_001917"
     CDS             15607..16464
                     /locus_tag="LQB69_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3526067.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            16486..16995
                     /locus_tag="LQB69_001918"
     CDS             16486..16995
                     /locus_tag="LQB69_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3526068.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            17032..17433
                     /locus_tag="LQB69_001919"
     CDS             17032..17433
                     /locus_tag="LQB69_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526069.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            17545..17898
                     /locus_tag="LQB69_001920"
     CDS             17545..17898
                     /locus_tag="LQB69_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526070.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            17932..18375
                     /locus_tag="LQB69_001921"
     CDS             17932..18375
                     /locus_tag="LQB69_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL3526071.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            18392..19477
                     /gene="uxuA"
                     /locus_tag="LQB69_001922"
     CDS             18392..19477
                     /gene="uxuA"
                     /locus_tag="LQB69_001922"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL3526072.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            19470..20183
                     /locus_tag="LQB69_001923"
     CDS             19470..20183
                     /locus_tag="LQB69_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3526073.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         6968 a         3138 c         4253 g         6024 t
ORIGIN      
        1 aactcgagga ggctatctga cgattcaata ttcattaatt taaagtttgg gtgactatta
       61 cttatttctc atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag
      121 ttacgccagt tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct
      181 ttcgtgtttc tctagtataa cagaataaga ggaacaagtc cagaagggat atttttagtc
      241 ttttttaaaa taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac
      301 ggcaagtgaa aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg
      361 atagaaataa aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg
      421 ttttggtaca aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa
      481 ataagaagga atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa
      541 tttgtttatt tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc
      601 atcactttac ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca
      661 attttttcta gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc
      721 atttttacta gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca
      781 gaaacttccc cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga
      841 ttggttgaat caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa
      901 gcttgaacaa gcttggagaa attttcccct tcatttgctt ctttttttgc tttatctgct
      961 tcttctttag tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa
     1021 gcagtgtcta gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc
     1081 agcatctgtt taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt
     1141 tccttgtagt ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt
     1201 gaagaaatct cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat
     1261 agtgtttgat tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt
     1321 ccacctttaa acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt
     1381 gcacatgctg aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc
     1441 ttattaaaaa cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc
     1501 tgtagatatt cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt
     1561 tttcacgaga caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg
     1621 atcaggatta ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga
     1681 attcttgtca ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca
     1741 attgccggta cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag
     1801 gtcgaaggaa aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat
     1861 ttagcgggag gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat
     1921 ttgacggggg cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt
     1981 tttattttat ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag
     2041 catcagatgc ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct
     2101 cttttgcacg gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca
     2161 ggttgctttt tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct
     2221 catattactt ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa
     2281 gaacaaggtt ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac
     2341 tttggggctg aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg
     2401 ttactctatg catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag
     2461 atgaagaaag caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa
     2521 aaagtaaaca aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt
     2581 cgtgttctca tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg
     2641 agagtgatag aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata
     2701 attgcagcaa tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca
     2761 tttactgtga aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca
     2821 gtcaaaccaa acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg
     2881 gatggtaatg gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg
     2941 accgctggta ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag
     3001 agtaaagata aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat
     3061 ttgaaaccaa gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt
     3121 gaaagcagta cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt
     3181 agcacagaaa acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca
     3241 ggaagcacga gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc
     3301 atgagtagtg agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat
     3361 aatgaacttg atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc
     3421 aacctaaaaa gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa
     3481 aaagcgaaag aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg
     3541 aacaagctaa ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt
     3601 cgttctctct tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa
     3661 acgatagatt tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta
     3721 gctttgccag atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca
     3781 caaatggaaa ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg
     3841 aaaaatcagt aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat
     3901 aatagataaa aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa
     3961 gtatatcaat ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat
     4021 atcgctttag atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa
     4081 tcgttttgcg aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata
     4141 aaagtcgagt tttcttctca gtttttactg agtaaagttt attctatact tttagaggga
     4201 acgattggtt atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat
     4261 cttagtcaaa aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata
     4321 ctattttgaa aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag
     4381 aagaataccg tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc
     4441 attctgatat attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac
     4501 atgcgccata tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt
     4561 tgcaacgaat gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg
     4621 tgcatccatt gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag
     4681 caaacttctg gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt
     4741 ttggtgtaat gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag
     4801 aaagttgggg ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt
     4861 gttttacaac ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt
     4921 caagggtttt tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag
     4981 attttcagca tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag
     5041 ctcgcagaat tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat
     5101 actgtatgtt agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc
     5161 aatcaaagta tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg
     5221 aacgcccaat aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt
     5281 atcctatcga caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac
     5341 aacctacaaa aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga
     5401 aaaatagatc catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg
     5461 ccaaaattaa gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag
     5521 caagagttcg ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc
     5581 ttcgctaaat ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa
     5641 agaaggggat gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg
     5701 aacaagattt ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt
     5761 caattttagt aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc
     5821 gataaacggt atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc
     5881 agaacctgta tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata
     5941 ataacatgaa gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc
     6001 acgtataatg gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc
     6061 acaagtatgt caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt
     6121 tttgattctt cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc
     6181 aaaacatacg aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta
     6241 gtgccacaaa caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat
     6301 tttgtgagaa aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact
     6361 gaattggact gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg
     6421 actgtgaagt ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca
     6481 acttatgata atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg
     6541 gataaacgat ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga
     6601 atggatactg gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta
     6661 tttttatggg ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc
     6721 tcctagaaac ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt
     6781 tggtcatctt atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca
     6841 tttcttccga atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg
     6901 aaacgaaatc gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg
     6961 ttactagcac agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa
     7021 cagctacaaa aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg
     7081 ggacagaaaa tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt
     7141 acttcggtaa acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat
     7201 taatagagtt attagtcact gagcttgctg ttaatgatta tggtgtagaa aaagagatta
     7261 tcaaacatct gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt
     7321 tgatgtcgat gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct
     7381 atccaaatac aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg
     7441 atgtatatgc atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat
     7501 ttcgagggaa ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg
     7561 ggtataattt attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga
     7621 cctatggaca atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc
     7681 ggccatctca aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg
     7741 tattagcgaa agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat
     7801 caattgatga acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg
     7861 ccataaaaaa tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc
     7921 ttttgaaaca atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta
     7981 cgattggaat gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag
     8041 tacctgtgtc taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat
     8101 tagtagaagg agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac
     8161 atgtaacgga ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg
     8221 aaaacaataa aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag
     8281 aaaattatat gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac
     8341 cgactgaagg gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc
     8401 cagtagctcc accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg
     8461 aacctcgaga ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac
     8521 aaaaaaagac agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc
     8581 ttctcattta ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa
     8641 gaaagagagg gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc
     8701 atcgtagtta ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc
     8761 ttgcttgtta gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat
     8821 gatttccagc aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag
     8881 tacaaaaata ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt
     8941 cttttctgga tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat
     9001 ttggaatatc tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca
     9061 ccggaagcaa aacatcctac accagttatc caattgaacg aagccattca agcagctaaa
     9121 aaagagataa aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt
     9181 gcacaaatta gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat
     9241 catcttgatc cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg
     9301 ttggatcctt ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa
     9361 tgtggatggg cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta
     9421 gccttggtca gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca
     9481 aatacgttta cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa
     9541 gtcacaaaag tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat
     9601 ttcagcaata aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa
     9661 agtcttatga tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat
     9721 ttcaataaac aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag
     9781 atggcaaggg gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct
     9841 cttttacgta aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa
     9901 tccgaagcct gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg
     9961 ttccactaat attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa
    10021 aatcaatgca ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc
    10081 cttttttata attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg
    10141 atgtgaataa aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta
    10201 atgattcagc aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa
    10261 aatatgggaa tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc
    10321 gatgtatcct aaactttttt attttacata acaatcatat caactgtgaa ataaaattac
    10381 aaatgataac ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca
    10441 aaattttttt taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa
    10501 attttttact actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc
    10561 aatacaacct tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc
    10621 tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg
    10681 ttttatggat ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg
    10741 tcgaacttta gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata
    10801 tagagagtcc aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc
    10861 tgtttcttca gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc
    10921 ttggctagta ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat
    10981 tgtttctttt gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa
    11041 agaagcttcc atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg
    11101 caaaaattta tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata
    11161 gacacgttta gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa
    11221 cttttttgtg tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag
    11281 ctcagtatct gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg
    11341 gatcaacatg gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg
    11401 aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact
    11461 tactttgctt ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc
    11521 gttgtacatt gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca
    11581 taataagatt atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa
    11641 aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga
    11701 gggtataaga aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg
    11761 attgaattta gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc
    11821 aagtacagct gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga
    11881 atttctagct acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat
    11941 aaccgaaaac atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga
    12001 aggcggaaac tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga
    12061 aatcggttac ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg
    12121 cttaatgatt ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt
    12181 agcaattgaa agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat
    12241 gatccataat ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt
    12301 acaaaaaagt gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc
    12361 ggtgattcgc ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt
    12421 aagtaattat cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc
    12481 gctagaaatg gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc
    12541 acaggaagaa gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg
    12601 acatgaaata gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt
    12661 ccagcagttt cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg
    12721 ttcatttctt tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag
    12781 gattcaatat cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca
    12841 aagatgaact gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag
    12901 cagcaaaaga acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg
    12961 tagcagaaaa taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag
    13021 caagagtcaa ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta
    13081 acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc
    13141 gtaacggttt tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg
    13201 aaagctttgg tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt
    13261 gtttatgtgc gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc
    13321 agatgaaaga aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag
    13381 cgatgattaa tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag
    13441 aagaaccggg acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac
    13501 atatatcggc ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg
    13561 tcgaacagat cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata
    13621 taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta
    13681 tttatatgaa gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa
    13741 aagagtactg gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt
    13801 tttgaatgac gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc
    13861 tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt
    13921 ggcggaggaa aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc
    13981 ggagtcatcc ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat
    14041 aaggagaatg gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt
    14101 ataacagatc cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca
    14161 gatacactag caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat
    14221 gaatcatttt tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat
    14281 cattctgcta aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag
    14341 ctttcagaga ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca
    14401 agaaatgtgg cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat
    14461 cgttttctac atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt
    14521 cgctgggaaa aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct
    14581 ctagaacaaa tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt
    14641 attgattcga aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata
    14701 cagtcaatga tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga
    14761 aacattaagt gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg
    14821 ctggttaggt ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt
    14881 cgtcgggttg atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat
    14941 gatttatatg ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc
    15001 agctatcgca gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt
    15061 tgcggtgcca gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac
    15121 ttttttaggt aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg
    15181 atcaattatt ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat
    15241 tgctttatat ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt
    15301 cgttatccac attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac
    15361 tgtatacgtt atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag
    15421 tgttgttttg aaatctttag atattacgat ggttacttat gccatttttg ggattattat
    15481 cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa
    15541 caattcagca tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa
    15601 tgggaaatgg ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa
    15661 gatgtaacaa aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa
    15721 cgttacttgg cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa
    15781 gatgaagact ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta
    15841 agctggggtg gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa
    15901 gaagagtatg aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg
    15961 ggattgatgg gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat
    16021 atttttatcg caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt
    16081 ccttttatct gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct
    16141 ttcactatgg gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt
    16201 gaaatgctat cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa
    16261 gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt
    16321 gatcaaatcg tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat
    16381 acgaaaaaag gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg
    16441 ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat
    16501 ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa
    16561 agtggagtga attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga
    16621 gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag
    16681 aaagctaaat tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca
    16741 aaaacagtcc aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat
    16801 attggaggga ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat
    16861 ttaaataaat cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat
    16921 atttattttc aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag
    16981 tttgatttgt cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag
    17041 aacgagctgt ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca
    17101 aaaaagagta aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca
    17161 gatgtgacag tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga
    17221 gatatcgaaa aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta
    17281 ggaagttatt tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta
    17341 ttgtccttca ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat
    17401 cctcatcacg tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg
    17461 agctactttt tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt
    17521 cgaaatactt catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt
    17581 gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga
    17641 tcgggtatga aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg
    17701 ctacagtttt cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag
    17761 ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc
    17821 tatttgacca aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt
    17881 attgctcttt ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa
    17941 atcatgatcg ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt
    18001 gtcgggacat cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca
    18061 ttcggagaga aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta
    18121 tttgttgatc taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg
    18181 actgaagaaa agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta
    18241 gaagcaatca atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc
    18301 aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat
    18361 gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga
    18421 aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat
    18481 tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt
    18541 gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca
    18601 cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact atatacgaac
    18661 aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt
    18721 tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata
    18781 cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca
    18841 gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct
    18901 ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt
    18961 agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga
    19021 tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa
    19081 aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg
    19141 ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt
    19201 tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc
    19261 ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt
    19321 cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg
    19381 atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat
    19441 aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata
    19501 tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac
    19561 aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa
    19621 tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc
    19681 gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga
    19741 aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg
    19801 cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct
    19861 tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct
    19921 ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact
    19981 cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc
    20041 atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat
    20101 atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag
    20161 aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt
    20221 cctgcacgac tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct
    20281 tatttgtagt gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga
    20341 tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt ttc
//
LOCUS       DAFFFQ010000052        20372 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000052
VERSION     DAFFFQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.052"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..189
                     /locus_tag="LQB69_001924"
     CDS             <1..189
                     /locus_tag="LQB69_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3526074.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="LQB69_001925"
     CDS             complement(1055..1585)
                     /locus_tag="LQB69_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL3526075.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="LQB69_001926"
     CDS             complement(1582..2199)
                     /locus_tag="LQB69_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL3526076.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2805..3002)
                     /locus_tag="LQB69_001927"
     CDS             complement(2805..3002)
                     /locus_tag="LQB69_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL3526077.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYK"
     gene            complement(3180..4712)
                     /locus_tag="LQB69_001928"
     CDS             complement(3180..4712)
                     /locus_tag="LQB69_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3526078.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="LQB69_001929"
     CDS             complement(4848..5933)
                     /locus_tag="LQB69_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3526079.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="LQB69_001930"
     CDS             complement(5948..6667)
                     /locus_tag="LQB69_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL3526080.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="LQB69_001931"
     CDS             complement(6664..7974)
                     /locus_tag="LQB69_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL3526081.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="LQB69_001932"
     CDS             complement(8001..8591)
                     /locus_tag="LQB69_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL3526082.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="LQB69_001933"
     CDS             complement(8909..9970)
                     /locus_tag="LQB69_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3526083.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="LQB69_001934"
     CDS             complement(10538..11605)
                     /locus_tag="LQB69_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL3526084.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="LQB69_001935"
     CDS             complement(11625..12104)
                     /locus_tag="LQB69_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL3526085.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="LQB69_001936"
     CDS             complement(12121..12582)
                     /locus_tag="LQB69_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3526086.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="LQB69_001937"
     CDS             complement(12596..13846)
                     /locus_tag="LQB69_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL3526087.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="LQB69_001938"
     CDS             complement(14224..15675)
                     /locus_tag="LQB69_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL3526088.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="LQB69_001939"
     CDS             complement(15996..16760)
                     /locus_tag="LQB69_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL3526089.1"
                     /translation="MIDLHCHILPGIDDGAESLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="LQB69_001940"
     CDS             complement(16788..17486)
                     /locus_tag="LQB69_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL3526090.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="LQB69_001941"
     CDS             complement(17498..18277)
                     /locus_tag="LQB69_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL3526091.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="LQB69_001942"
     CDS             complement(18293..19237)
                     /locus_tag="LQB69_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3526092.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="LQB69_001943"
     CDS             complement(19283..20062)
                     /locus_tag="LQB69_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526093.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         6483 a         4051 c         2621 g         7217 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatctattt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagctt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac aacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccgaatcgt acgatattgt
    17401 tcagcgattg gtgaagaggg attggtgagt gtgaccaaac tgactgcatg agtttgggct
    17461 gctttttgtt tctgtgttcg tgccattttc tttcctccta gactcttgag cggcttctgc
    17521 gtgatggcgc tgttttatcg tttgtagctt ttggttgtgt ctgagctggt ccttttttac
    17581 ggataccagc attcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagccc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatcacgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctcagcaa
    19321 cagtttgtgc ttttgttgcg tatgctgcgc ttattttttc attccggatc tgctctattt
    19381 gactgagcac cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttta
    19441 acatcgtatc gaataactga tctagttgcg tgacttgcgc gatctgaata tcagcgaatc
    19501 ctagatattg cataacaacc gttttccaag gactatcttt aaaaaagctc atattttcac
    19561 gtacacgagc gacatctgca cttgtcacct tttctttgat gatgacatcg tctacagctt
    19621 gctgccagtt tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt
    19681 ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag
    19741 cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg
    19801 atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaaattt
    19861 tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt
    19921 gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga
    19981 tatggtatcc aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg
    20041 acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga
    20101 tataattcac ttacataaaa atgacagctt tttatttaaa aaaacataat taaactaata
    20161 tattcacaga aatatttgta aacaagtttt ccctatataa tcataccaca aactgttttc
    20221 ttgactaggg caatttacga tttaaaagta aataaaacat taataagata aaaattaatc
    20281 gatttcattt ttatttccat aattttcata ggtgttttta aaagttacac aaaaaataga
    20341 ataacaaaaa aattaacatt gaattaataa ta
//
LOCUS       DAFFFQ010000053        20181 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000053
VERSION     DAFFFQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.053"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="LQB69_001944"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="LQB69_001944"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL3526094.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="LQB69_001945"
     CDS             1011..2258
                     /locus_tag="LQB69_001945"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL3526095.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="LQB69_001946"
     CDS             2334..2678
                     /locus_tag="LQB69_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526096.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="LQB69_001947"
     CDS             complement(2795..3121)
                     /locus_tag="LQB69_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526097.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="LQB69_001948"
     CDS             complement(3494..4696)
                     /locus_tag="LQB69_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526098.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="LQB69_001949"
     CDS             4813..5328
                     /locus_tag="LQB69_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3526099.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="LQB69_001950"
     CDS             complement(5527..5973)
                     /locus_tag="LQB69_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL3526100.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LQB69_001951"
     CDS             complement(6020..7498)
                     /gene="msr(C)"
                     /locus_tag="LQB69_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL3526101.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="LQB69_001952"
     CDS             complement(7852..8481)
                     /locus_tag="LQB69_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3526102.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="LQB69_001953"
     CDS             complement(8465..8929)
                     /locus_tag="LQB69_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3526103.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="LQB69_001954"
     CDS             complement(9416..10684)
                     /locus_tag="LQB69_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL3526104.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="LQB69_001955"
     CDS             complement(10915..11652)
                     /locus_tag="LQB69_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526105.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="LQB69_001956"
     CDS             complement(11719..12288)
                     /locus_tag="LQB69_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL3526106.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="LQB69_001957"
     CDS             12416..13288
                     /locus_tag="LQB69_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3526107.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="LQB69_001958"
     CDS             13630..14502
                     /locus_tag="LQB69_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3526108.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="LQB69_001959"
     CDS             14499..16457
                     /locus_tag="LQB69_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526109.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="LQB69_001960"
     CDS             complement(16708..17559)
                     /locus_tag="LQB69_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526110.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="LQB69_001961"
     CDS             17682..18149
                     /locus_tag="LQB69_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL3526111.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="LQB69_001962"
     CDS             complement(18412..18894)
                     /locus_tag="LQB69_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL3526112.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="LQB69_001963"
     CDS             complement(18994..19857)
                     /locus_tag="LQB69_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL3526113.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6066 a         4155 c         3664 g         6296 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgatg aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tacttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tacactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt t
//
LOCUS       DAFFFQ010000054        19995 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000054
VERSION     DAFFFQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.054"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            4..816
                     /locus_tag="LQB69_001964"
     CDS             4..816
                     /locus_tag="LQB69_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL3526114.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LQB69_001965"
     CDS             813..1613
                     /locus_tag="LQB69_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL3526115.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LQB69_001966"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LQB69_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL3526116.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LQB69_001967"
     CDS             3110..4819
                     /locus_tag="LQB69_001967"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL3526117.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="LQB69_001968"
     CDS             5031..9383
                     /locus_tag="LQB69_001968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL3526118.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="LQB69_001969"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="LQB69_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL3526119.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="LQB69_001970"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="LQB69_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL3526120.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="LQB69_001971"
     CDS             11221..11514
                     /locus_tag="LQB69_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL3526121.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="LQB69_001972"
     CDS             11511..11822
                     /locus_tag="LQB69_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL3526122.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="LQB69_001973"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="LQB69_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL3526123.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="LQB69_001974"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="LQB69_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL3526124.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="LQB69_001975"
     CDS             complement(14623..14844)
                     /locus_tag="LQB69_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3526125.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17655
                     /locus_tag="LQB69_001976"
     CDS             15292..17655
                     /locus_tag="LQB69_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HBL3526126.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKLGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRNDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFHSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAATLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            18023..18946
                     /gene="truB"
                     /locus_tag="LQB69_001977"
     CDS             18023..18946
                     /gene="truB"
                     /locus_tag="LQB69_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL3526127.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18950..19891
                     /gene="ribF"
                     /locus_tag="LQB69_001978"
     CDS             18950..19891
                     /gene="ribF"
                     /locus_tag="LQB69_001978"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL3526128.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKKDEQATAEYFSQQ"
BASE COUNT         6862 a         3512 c         4389 g         5232 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatccag tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa accagaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatatcga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaagcgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga gcctttcttt attgaaggaa atgatccaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa tcaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaattgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac taggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt ttttgcaagt
    16741 tacgaatcat tcttgcgagt ggttgactct atgttgactc agcacttcaa atggatacgg
    16801 aaatcacatg acctttcttg gcgaaatgat tatccttcgc taaatctcat tgcaagctca
    16861 acagtcttcc agcaagatca caatggatat agccaccaag atccaggaat attgacacat
    16921 ttggcagaaa agaaggcgga gttcatccgt gaatatctgc ctgcggatgc aaatacattg
    16981 ctcgcagtca tggataaagc attccactcg tcagaaaaaa tcaatttgat catctcttcc
    17041 aaacatccgc gtgctcaatt ttactcagca gaagaagcag ccgtcttagt gaatgaaggc
    17101 ttgaaaatca ttgattgggc ttcgacagca aaagaggagg aacctgaatt ggtcattgct
    17161 gctgcaggaa cggaatctaa cttagaagca ttagcagctg caactttgct gctggaagaa
    17221 ttcccaaaac taaaaattcg gttcattaat gtcgtagatt tattgaaatt acgtcatcca
    17281 agtcaagatc cgcgtgggtt gagcgatgaa gaatttgatc aatattttac aaaagacaaa
    17341 ccgatcttat ttgctttcca cggttatgaa acattggtcc gcacgatttt ctttgatcgt
    17401 cataaccatc atttaatgat tcatggctac aaagaaaatg gagatatcac gacaccattt
    17461 gacatgcgtg tagtaaatga acttgatcgt tatcatttgg caaaagacgc agctttaaaa
    17521 atcaaaggaa gtcaagcaga agattttgcg gaaaaaatgg atcagaaatt acaagaacat
    17581 caaaactata ttcgtgaaaa tggtattgat ctgccagaag tattggattg gaaatggaaa
    17641 aatcttgatc aataaaatga taaaatcgat cccgcaaaac agaatttttt acattgatta
    17701 agaaactgaa aaaataaata aaaaaaacgg aaaaaagact ggaagaatgg acagtctttt
    17761 ttttcgtttt tgggaagtga aaaccaaact atttacctgt aagccaccca taaatccaat
    17821 caaatggtgc aaagaaaaaa tcttccattc tattccctcc tttaaaaaaa cataacatga
    17881 tttctattta cgtgagcatg aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt
    17941 cattatgatg tcaaattcgt cagtaattga tgcaatgtgt tataataatt cagttaaatc
    18001 aaaaatgaaa tggagaaaag acatggacgg gttattacct ttatggaaag aacgcggaat
    18061 gacaagtcat gattgcgtat tcaaattaag aaaaatctta catacgaaaa aaatcggaca
    18121 tgggggtacg cttgacccag acgtagacgg tgtgttgcct atttgtattg gaaaagccac
    18181 aaaagttatc gagtatttga cagattctgg gaaaatctat gtaggcgaga taactctagg
    18241 tttcagtaca acgacggaag atcgatccgg agaacgggta gaaacaaaac cagtagatat
    18301 gtctttatct gaagaaaaaa tagatgaagt gatggcttca tttattggtg agatcaccca
    18361 gataccacca atgtactcag cggtaaaagt caacggtaag cggttatatg aatatgcccg
    18421 caataatgaa acagtggagc gtcctgttag aaaggctcag atttaccgtt ttatccggac
    18481 aagtgaattg acaagagacg aagtgacagg aaccatttct tggcgcttcg aagtagaatg
    18541 cgggaaagga acctatgtcc gcacattagc tgtagataca ggcaagaaac ttggttaccc
    18601 tgcgcacatg tctgatctga cccgaacagc aagtgccggt atgtcccaga aagatgctct
    18661 gacattagaa gaagtagctc aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc
    18721 aatagaaaaa ggagtgaaac gattcaaacg agtagatatt actgatgaag tatggcagaa
    18781 agtaaaaaac ggtatgagac ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga
    18841 agaaattgct ttgttttata aaggcaaagt tgtcagtatc tatcagccaa acccgaaaga
    18901 aaaaaacaaa ttaaaaccta gtaaagtatt gagaaatgag gaataacgaa tgaatatcat
    18961 caagatcaga catccttacc aatcagaaca aattccagaa gacgatgtcg ttttagtact
    19021 gggatttttt gacggcgtgc atattggaca ccagaaagtc ataaagacag gtatggaaat
    19081 tgcacaaaaa gaaggattga aattagcatt gatgactttc aatcagcatc catcgattgt
    19141 ctttaaaaag atcgatccta gcagcgtgaa atatttgact agccttaagc aaaaagaaga
    19201 aaaaatggcc gctttgggta tagattattt atatgaagtt gattttactt cttcttttgc
    19261 tcatttagca ccgcaggaat ttgttgatca atacatcgta gggttacatg caaaatatgc
    19321 tgtttccggt tttgattata cgtatggacc aaaagacatt gcagatgtcg ctcattttcc
    19381 tgaatatgcc aaaggaagat tcaaaatcgt cacagtttct aaagaagaag aacaaggtga
    19441 aaaaatcagc tctacccgta ttcgaggatt actagacagc ggacaagtaa aagaagcagc
    19501 aaaactatta ggttgcacat atgaggtaga aggggtagtt gtccatggcg atgcacgagg
    19561 cagactacta ggattcccta cagcaaatgt caaagtaaag agcacagttc atttgcctaa
    19621 agaaggtgtt tatgtatcgg aaataaaaat tggggaaact tggtatccag ccatgggttc
    19681 aattggacat aatgatacat tcggtgaagg acgggagctg actgtagaat tgtatatttt
    19741 ggactttaat caagatatct atggtgaaca tgtcgcagta cgctggaatc aatttttaag
    19801 agatcaagtg aaattttcta gtgtagagga attgattgaa caactcaaaa aggatgaaca
    19861 agcaacagct gaatattttt ctcagcaata aaacattatg cataaaatct cttgtttatg
    19921 tataactagc aatttcaata aaatgggtga taaataagcc caggaaaggg gctgtgacaa
    19981 aagtcttgtc acagc
//
LOCUS       DAFFFQ010000055        19186 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000055
VERSION     DAFFFQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.055"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(37..1188)
                     /locus_tag="LQB69_001979"
     CDS             complement(37..1188)
                     /locus_tag="LQB69_001979"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL3526129.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="LQB69_001980"
     CDS             complement(1201..1638)
                     /locus_tag="LQB69_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526130.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="LQB69_001981"
     CDS             complement(1653..3419)
                     /locus_tag="LQB69_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL3526131.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="LQB69_001982"
     CDS             3683..4360
                     /locus_tag="LQB69_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526132.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="LQB69_001983"
     CDS             4363..5490
                     /locus_tag="LQB69_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526133.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="LQB69_001984"
     CDS             complement(5690..6130)
                     /locus_tag="LQB69_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526134.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="LQB69_001985"
     CDS             complement(6141..8174)
                     /locus_tag="LQB69_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL3526135.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGLFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="LQB69_001986"
     CDS             complement(8176..9879)
                     /locus_tag="LQB69_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526136.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LQB69_001987"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="LQB69_001987"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL3526137.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="LQB69_001988"
     CDS             complement(10962..11987)
                     /locus_tag="LQB69_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL3526138.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="LQB69_001989"
     CDS             complement(12196..13065)
                     /locus_tag="LQB69_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL3526139.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="LQB69_001990"
     CDS             complement(13524..15416)
                     /locus_tag="LQB69_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526140.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="LQB69_001991"
     CDS             complement(15413..17140)
                     /locus_tag="LQB69_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526141.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="LQB69_001992"
     CDS             complement(17156..17806)
                     /locus_tag="LQB69_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL3526142.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="LQB69_001993"
     CDS             18010..18306
                     /locus_tag="LQB69_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL3526143.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18498..19118
                     /locus_tag="LQB69_001994"
     CDS             18498..19118
                     /locus_tag="LQB69_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL3526144.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
BASE COUNT         5455 a         3954 c         3381 g         6396 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa aagaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaata atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg attctccttt
    17821 ctttcaagat gacacattgt catatgacgc tatgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt tataacaatc atagcaataa
    18481 aaggagaagg taagcatatg gcatctgttt tagtagtcaa aggtcatccc ctaactgcag
    18541 aagaatcgcg cacagttaag gcattgacgt cttttttaac aagttataaa gaaaatcatc
    18601 cagatgatga agtgactgtt ttggaacttt atcgcgatga tattccggaa atcgatgaag
    18661 aattattatc aggttgggaa gcattacgtg ctggtgcaga attcacttcc ttatcagaaa
    18721 gtcaacaaca aaaaattgct cgtttcaatg aattgactga tcagttcctt gctgctgata
    18781 aaatcgttat cgcaaacgcc ttatggaatc taaatatccc aacaaaatta aaagcttggt
    18841 tcgataccgt caatgtagct ggaaaaacgt tccgctatac agaaaacggg ccagaaggat
    18901 tagtgacagg taaaaaagca ctgcacatcc aatctaatgg cggtgtatac aacggacaag
    18961 actttgcctc tcaatatgta aaaggtattt taaactttgt aggcatcgat caagtagatc
    19021 agctattcat cgaaggaatc gattatgacc ctgaccgtgc agatgagttg atgcaaaatg
    19081 cattagataa agctgctgct ttaggtaaat cattttaaaa attgagataa atgcatacaa
    19141 aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc ttggat
//
LOCUS       DAFFFQ010000056        18966 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000056
VERSION     DAFFFQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.056"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(21..251)
                     /locus_tag="LQB69_001995"
     CDS             complement(21..251)
                     /locus_tag="LQB69_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526145.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="LQB69_001996"
     CDS             complement(687..1253)
                     /locus_tag="LQB69_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL3526146.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="LQB69_001997"
     CDS             complement(1253..2209)
                     /locus_tag="LQB69_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL3526147.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="LQB69_001998"
     CDS             complement(2209..2865)
                     /locus_tag="LQB69_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL3526148.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="LQB69_001999"
     CDS             3235..5253
                     /locus_tag="LQB69_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3526149.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="LQB69_002000"
     CDS             5680..8094
                     /locus_tag="LQB69_002000"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL3526150.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="LQB69_002001"
     CDS             8442..9995
                     /locus_tag="LQB69_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL3526151.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="LQB69_002002"
     CDS             complement(10376..12160)
                     /locus_tag="LQB69_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526152.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="LQB69_002003"
     CDS             complement(12175..13896)
                     /locus_tag="LQB69_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526153.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="LQB69_002004"
     CDS             13996..14757
                     /locus_tag="LQB69_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3526154.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="LQB69_002005"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="LQB69_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18966
                     /locus_tag="LQB69_002006"
     CDS             16664..>18966
                     /locus_tag="LQB69_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526155.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6231 a         3365 c         3805 g         5565 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtttaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat atcagctggt
    18961 agaaac
//
LOCUS       DAFFFQ010000057        17499 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000057
VERSION     DAFFFQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.057"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            221..553
                     /locus_tag="LQB69_002007"
     CDS             221..553
                     /locus_tag="LQB69_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL3526156.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYVGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1173..1514
                     /locus_tag="LQB69_002008"
     CDS             1173..1514
                     /locus_tag="LQB69_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL3526157.1"
                     /translation="MKNYDPNIRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEEDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1628..1867
                     /locus_tag="LQB69_002009"
     CDS             1628..1867
                     /locus_tag="LQB69_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526158.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1934..2173
                     /locus_tag="LQB69_002010"
     CDS             1934..2173
                     /locus_tag="LQB69_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526159.1"
                     /translation="MNCKESVNWKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKTSSNCQLLLEVS"
     gene            2300..2989
                     /locus_tag="LQB69_002011"
     CDS             2300..2989
                     /locus_tag="LQB69_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL3526160.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDDVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2995..4161
                     /locus_tag="LQB69_002012"
     CDS             2995..4161
                     /locus_tag="LQB69_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL3526161.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFALSMILLLSFLWLL
                     VRKCELTNGQLTFLWAFLAIMCQSFMEETLATISWVPIVFLMLAMATHQYKTTR"
     gene            4243..4914
                     /locus_tag="LQB69_002013"
     CDS             4243..4914
                     /locus_tag="LQB69_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL3526162.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFRLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4966..6942
                     /locus_tag="LQB69_002014"
     CDS             4966..6942
                     /locus_tag="LQB69_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3526163.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAHAEDVTQKTPPEKVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVIYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTEQSKQTPLKGAKFELYKGEPGKGSKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQVGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6955..7707
                     /locus_tag="LQB69_002015"
     CDS             6955..7707
                     /locus_tag="LQB69_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3526164.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVSKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7723..8478
                     /locus_tag="LQB69_002016"
     CDS             7723..8478
                     /locus_tag="LQB69_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526165.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLVSNGETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSEKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            8491..8751
                     /locus_tag="LQB69_002017"
     CDS             8491..8751
                     /locus_tag="LQB69_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526166.1"
                     /translation="MKLIKLLLFNLSCLTAVFLYCGLLFSQLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8748..10838
                     /locus_tag="LQB69_002018"
     CDS             8748..10838
                     /locus_tag="LQB69_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3526167.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRQDAVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVDYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10874..12931)
                     /locus_tag="LQB69_002019"
     CDS             complement(10874..12931)
                     /locus_tag="LQB69_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL3526168.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(13075..13266)
                     /locus_tag="LQB69_002020"
     CDS             complement(13075..13266)
                     /locus_tag="LQB69_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526169.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            13454..13909
                     /locus_tag="LQB69_002021"
     CDS             13454..13909
                     /locus_tag="LQB69_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526170.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13906..14148
                     /locus_tag="LQB69_002022"
     CDS             13906..14148
                     /locus_tag="LQB69_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL3526171.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14166..14468
                     /locus_tag="LQB69_002023"
     CDS             14166..14468
                     /locus_tag="LQB69_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526172.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGIILAAASIVVSLFGMFGQTPQGL"
     gene            14537..16600
                     /locus_tag="LQB69_002024"
     CDS             14537..16600
                     /locus_tag="LQB69_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526173.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMDQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            16600..>17499
                     /locus_tag="LQB69_002025"
     CDS             16600..>17499
                     /locus_tag="LQB69_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL3526174.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNK"
BASE COUNT         6147 a         2746 c         3563 g         5043 t
ORIGIN      
        1 aaaaatacgt gatattacga tgaatctatg cctaagcata atgttataaa aatgtccgtg
       61 tggctctttg ccgtatgatg aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc
      121 ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat aacaagaaaa
      181 acttgttaaa aatatttaaa gtaagtgggt ggattatttt atgtcatata caggaatttt
      241 gagtctcgaa gatatttgtc actacggtaa acggtgtacc gctacagaga agatcacgaa
      301 gaaactatca actggccaaa ataaaaccgt tgtgcagtgt aagaaatata taatccaaaa
      361 agacaaagtc tcagaggaaa tgatttatta cgttgggaaa caaaagcaaa taattttaaa
      421 agatcctatc cctttaaaag agctatatcc aacaatcaaa catgtttatg accaaaacgg
      481 tgtattgata ggtcgaagaa aaaatggagt cttacgttgt accgcaaaag ggatggggcg
      541 tttgatcagc tgaattgatc attcttataa cttgtatttt atcgtttttc gcttgcttga
      601 aaatattgtc gagacttcta aaagaactta gaatcactag aaaaataagg agtagacttg
      661 atctatcaac aattcccttc ggggagtgat ggggtaggtt tttacgattg gtaacgtaaa
      721 aaattggtca atcgagtaaa tgcccttgtt tggatagtgg tgaaatgaat ttgtgtaaat
      781 aatatgcaca gagtaaacaa gagggaattt agaagttaga caagttagaa tagcgcctag
      841 attaatcatg agcagctact aagggatcta ggctttgacg caaactcatg gaataaggag
      901 taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt
      961 agatacttgc gaggacgttg attattggat caatcgagca tttaccgatg ttctactagg
     1021 gtttctcgtt tcggacgcca taaacgagtc gatatttttt atgtattttt aagtaaacga
     1081 aaaacgataa aatatgaacg agtcctgaat aaagtgaaaa aattgatata aaacatagag
     1141 aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat
     1201 ggggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt
     1261 ttagaagagg cggaaattgt aaaggattag atgttttagc attagacgag gaagatttat
     1321 acgatcaaaa attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag
     1381 gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag
     1441 atacttggtc atatttgagt gattatattg tcagcgttga aatcattgaa tttgtagcgg
     1501 ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac
     1561 gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaattta tttaggagga
     1621 aaattagatg gcgcgaccag ttttagaggc gatggatatg gatactttga caaaaggaag
     1681 gtatgtacag ctgttagctc gtggttattc acctaaagat gtattcaaag ccctatctaa
     1741 aggaacagat atggaaaaag aaagattacg aaaagaattc gattattgga gaacccataa
     1801 tggaatcaag gatttgaaac cagcacggcc agtaattaaa cgaaagaaaa agataaccca
     1861 agactgaagt gtagagcgta tgtgatcata ggctttttta tttttgcaaa aaacagggaa
     1921 ggtaggaaaa ctcatgaatt gtaaagaaag cgtaaattgg aaaaaagtgt caaaagaaat
     1981 ggaagaaaag ttgctaaaaa tgatgaaaca aaaacacctt aaaagattat ctgtcatgca
     2041 atatatcaat gatatgcaga taacaggaaa ggagaaagcc tgtctattgg gatcaatgaa
     2101 aaattttgaa cagttgagac gaacttatgt gaaaacaagt tctaattgcc aactattatt
     2161 agaagtttct tgacgattat gatatgaaaa tgatcaatta tgatgtggca ttgttattga
     2221 ataaaaagaa tagtaaaatt taacgtggtt tatcttggtt agttgtaggt aaattaatta
     2281 atatatagaa ggtagataga tgaataaaaa caataaaact ttatttaatc cttacgtggg
     2341 cgtaatcatt gtttttctcc tttttagtta ttttgcttat caaggttatc aaaaggacaa
     2401 aatcgctgaa aaagtagatg atgtaacaaa aacaatccat attcaggaac aggctaaaac
     2461 ctatcaagat aatttaacca gtttaacgga tatttctaag agacaactga ttactaatca
     2521 aaaacaggca ttagaaaagg atctcaaagg tgaaattgct ggaaaacttg ttttttcttc
     2581 tacaggattt attctccctg tttttcaagg agcgaatagc gacacgctta gtttaggagt
     2641 agcttctact tattatccag atgctgaaat gggaaatgga aattatattc ttgctggtta
     2701 taacgtagat acaccaagcg ttttattatc cgatatgatt gatttatccg aaggagaaga
     2761 aattgatcta atcgatgctc attacaagta tcagtatgaa gtctccaaaa gaggaaaaat
     2821 ttatgattac agccaggtag ttcaatctaa ggaaaacaat tcctttttat ccttgccaaa
     2881 agcaggtaaa aaaccactac tcacactgtt agactttgaa gcaaataata tgaaaaagtg
     2941 cgatgtgatc cagtgcgaat tagtcaatgt agaaaaaatc aataaataag ggaagtgagt
     3001 cacattcaag aacttgtaga aaaattgatc aataaaatga gtgccatttt tctgttactg
     3061 ttggtatcca ttctttattt aatatggacg gtggggttaa atcctcctac gatattattt
     3121 atttatgata atgctgcaca gttagtgacg ttttttgtag gctgtttgtt tcttttgaat
     3181 ctgtacaagg taaagagaaa cgatctattc ttcattgcag caggcattct tttttacctt
     3241 ttcttttctt tttttagaag ctaccgagga gatactttct atggtgatct gatcgttccg
     3301 acaattattg ccattatttt gattgtaaaa tggacggaat ttactgtatt tgatcgtagc
     3361 ttgtatcttg tagtattcta tgtcttttta ctcgtaacgg tttatcgagt atttaccgag
     3421 ataccagtac cagaaggaca aagtatttgg aatccatcga acaaattatc agatatttgg
     3481 atcaatacaa atacgatagg tagttcttta atgacattag cccttcttat cacaggattt
     3541 gctagttcat ttggaaaatg gtacatgcga ttgctatcat taccagcgtt gattcttgct
     3601 ggggcaacga tttgggtttg ccaatcgaaa gcagcattat acgcgcttgt tatcttcgtt
     3661 atcctagaaa ttttacctaa gaagatcttt cgaaacacac acttgtcctt ttggatgtat
     3721 gcagcagttt cggttctagc tttaccgatt tcttattttg ctgcaatgtc agacaaagtt
     3781 catttattta caggacgaga agaaatttgg cgcaaattct atgaaacatt aggcgaaaaa
     3841 acaggacaaa tatttgtagg gatgaaacct ttctttttcc atagaggaaa tcagattcta
     3901 ggtaaccata atacgtatca cgcactcgtc aatcactatg gattgattgg ttttgctcta
     3961 tcgatgattc ttttgcttag tttcttatgg ttgctcgtaa gaaaatgtga gctgacaaat
     4021 ggacaactaa cattcttgtg ggctttttta gcgatcatgt gtcaatcttt catggaagaa
     4081 acattagcta caatcagttg ggtaccaatt gtgttcttaa tgttagcaat ggctacacac
     4141 caatataaaa ccaccagata aatgaaatag aatagtaata agagaaaaca ctttttcgtt
     4201 gtgaaaaagt gttttttgtt ttgtctgaaa ggaggcagat ggatgaaaaa atggttattt
     4261 ggttttttag gagtcgcttt aatcgtcgtt tgcagtgtgt ttggctatgt tagctaccaa
     4321 aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa agatgcccgt agatcaaatc
     4381 aatgcacatg caaagagtta taaggaggat gcaacgaacg tcaataatga tatgtctttg
     4441 gggcaaatgt tgtccataca aaaagaagca atcgaaatgg gcgtcaataa acaagtattt
     4501 gcacaaattc agattcctgc acttggttta gcattgccga tttttaaagg ggcaaatcaa
     4561 tacactttga gtcttggagc ggctacttat ttttatgaag atgctgaaat gggcaaagga
     4621 aactatgtcc tagcaggtca taacatggaa atgccaggtg ttttattttc tgatattcaa
     4681 aagttgtcat taggggaggt gatggactta gttagtaacg atggcgtcta tcgttacaag
     4741 gtaacgcgta aatttatcgt acctgggtac tttagattaa tcgatggcgt tcctgaagag
     4801 aactctttct tgtctttgcc aaagaaagga gagaaaccct tattaacgtt gtttacttgt
     4861 gtctatacgt cacaaggaaa agaaagatac gtggtacagg gagagctaca ataacaaggt
     4921 tttaatcaac gcgagtcgtt gaaatatgaa atgaggagag taaaaatggc aataaatcga
     4981 tatgtaaaaa aagctggaat gcttgttgga atgttaggaa tcattgcaag ctgctttcag
     5041 gggtatcaag cacatgcaga agatgtcacg caaaaaacgc caccagagaa ggtcaatatc
     5101 acggttcata aattaatgta cgatcaaagt acgcaattga atgttgatat tgacgggatc
     5161 aaaaatgatg gttatacgca cgatcagtat cctacaggtg taacgaaata caacaaggca
     5221 gattatggtg atgttgagtt tacgttagga aatattacgg atcaagtatt accgactgaa
     5281 gattctgatc taaccaacgc gaaagtggac gaaatcgtga aagacgtaga agacaaggga
     5341 tctaacagtg agtacgtcaa gaatgcaacc aataaaatta cttcagctgt agatgaaaat
     5401 ggtgagatta cttttgcgga tcaaccagcg tacgtgaact caaaaggaaa tgtgtatgtt
     5461 atttacgaga gtaagtccgc agcaggatta gtaactcaaa aagcaaaacc aatggtggtc
     5521 attgctccaa tgactgacaa cacaggttca ggttttttaa aagacattca cttatatcct
     5581 aaaaatattg tttctaagtt gagttttgaa ttaaccaaat tcggtgatga tggtacagag
     5641 caaagcaaac aaacccctct taaaggcgct aaattcgaat tgtataaagg agaacctgga
     5701 aaaggaagca aactaggcga tcttgtttcg gatgatcaag gaaagctgac tgctactgat
     5761 ttgacgttag gaaaatacta ttttgtggaa gttccttcag aagtcgtagt gggatcagat
     5821 aaagaaccaa cagccgatca atatttatta ggcgcagacg ctcgcaacga tgcgcacaat
     5881 aaattaacct ttgaaattac gaatgatggc gtgacaagtg acttaaaagc ttcatacgta
     5941 aactataaag caccagtgat cgataaaaca gtaacaaatg gaacaggaca agaacattct
     6001 ttccaagttg gtgatgccgt taattatcaa ggaacgattc atatcccaac agacattgca
     6061 ggcggagcag acggtatcac agtaaacggc gtgaaaagcg aaacttctcc ttacagtgta
     6121 tttaaatggg gggatacagc aggacaagga ctttcttatg tcgctgctaa agcaaatatc
     6181 aaagtaacaa ataaagatgg atcagtggta ttaaaagaaa atacagatta taagatccaa
     6241 aattcagaaa atggctttgt gatcgatttt atcgtaaata atggccaagt aagtgatact
     6301 gtagcaagtt tacacggaca agatcttcaa atgacttata acatgtatgt aaatgacagc
     6361 gcagctgttg ccaatccatt aacaaatagc gtggatttcg tttataacaa caatccattt
     6421 aatcaggaag aacaccacga aaaaacgaaa gctgacgttg tgacttatgg cgcgaaattc
     6481 ttaaaagtcg atagcggact atttggaaca ggaattaagg caactcctct tgaaggcgca
     6541 gaatttgcag ctaagaatgc agaaggtaag tactacggtg gacttactga tacagataaa
     6601 gatggtgtca aagaagcagt atgggtagac gatgtagcaa atgcagcaat cctaaaatca
     6661 gataaagagg gtcattttga aatcactggt ttaactgaag gagattactc actggaagaa
     6721 acaaaagctc ctgaaaacta tcaaaaatta acaaaagaga tctctttcaa agtagacaaa
     6781 gattcataca aagaagaaaa ccgaatcacg atcaaaaata atcaaaaagc ttccgtacca
     6841 atgactggta gcaacggttt ccaatcttac gtattgatct cttgcttgtt gcttggggca
     6901 ggcgcaattt ctgcagttgt ttactttaag aagaaagcct agaaggatca gctaatgatt
     6961 atcagacacc ctaagaaaaa gcgaatcatg ggtaaatgga tcattgcttt ctggttactt
     7021 tcagctgttg gtgtcttact tttgatgcct gctgaagcga gcgtggcgaa ataccaacaa
     7081 aatcagcaga ttgccgcaat cgatcgaaca ggaacggcag cagaaacgga ttcttctttg
     7141 gatgtttcta agattgagtt aggcgatcct gttggtattc ttacaattcc aagtatttct
     7201 ttaaaactac caatttatga cggaacttcc gacaaaattt tagagaatgg tgtagggatt
     7261 acggaaggaa ctggcgatat tacaggagga aacggtaaaa atcctttaat cgcagggcac
     7321 agtggacttt ataaagataa tcttttcgat gatttgcctt cagttaaaaa gggagaaaag
     7381 ttttatatca aagttgatgg tgagcagcac gcttatcaaa tcgatcggat tgaggaagtg
     7441 caaaaagacg aactacaaag aaactttgtc acttacttag aaccaaatcc caatgaagat
     7501 cgagtaacgc taatgacatg tacgcctaag gggatcaaca cacaccgttt cctagtgtat
     7561 gggaagcgag taacctttac aaaatcagaa ttaaaagatg aagaaaataa gaaacaaaaa
     7621 ttatcttgga aatggctttt aggaagtact gtttttcttt cagtcatgat cattggttcg
     7681 ctatttgttt ataaaaagaa aaaataaagg agggtaacca acatgagaaa tagaaaatgg
     7741 ctatttgttt gtgcagccgc tcttttttct ttcactgcct acacacagtt gacgtttgca
     7801 gacagtcaag aaagcacgga tagcacagaa caaacaatcc aaacgtcaga tagcagcacg
     7861 attcaaagca gcgatgaaac cgtcgattcg tcaacagtgg attcttcaga agaaccgaaa
     7921 attgctgaag acgatcaaat agtcaaacag accattcacg ttcaaaagat tatttcttca
     7981 gaacaattag acaaccaggg gcatgaaaaa caagaaggag taaatggcgc gaagttttct
     8041 gtttatgatg tatcggacat tttacaaaag atggatgtta aggatctcac tactgaccaa
     8101 attgaatccc aattaaaaga tcgagtgaaa aaactatcca gtgatcaatt gaagctagtt
     8161 tctaatggag aaacaaagac aattgatcaa caaacaggtg tctttgaatt ctcggtagaa
     8221 gtacaggcaa atcagaaaca agcttattat attgtcaatg aatcaagtcc tgaaaacatt
     8281 tctaatagcg aggatatttt gctattaaca cctgtttctg aaaaaaacgg tgaattctta
     8341 aaggatgtgt ggatttatcc taaatcagaa gcttcgcaac ctaaagaaga agtaaaaaaa
     8401 atcgtttcta ctggtgtaaa gaaaaacttc tttgagaatt gttgggattt tgttactcac
     8461 ttgttttcta gagattaagg aagaaataag atgaaactga tcaaattatt attattcaat
     8521 ctttcttgtt taaccgcagt ctttttgtac tgcggtttat tattttctca attatctatt
     8581 ttgccaagct accaactata cctaatgagg attttaaaca taccagctgc ttccatgccg
     8641 ctagatatta tggaaaagct cattaggaat ccattattta ctacgatcaa tttgatcgtt
     8701 attgtcggaa tttcactagt catttataaa aaagagggga tgaagggatg aattttaaac
     8761 actcgtggac cagacggtta aaatatgttt ttcccataat gatgatgtta ggtacattgt
     8821 ttgggttaaa aacagccaat gtctttgctg aaaaagttat tgtcgatccg tcaaatccaa
     8881 tccagaacgt aaagaataat tttattattc caaaagatgt tcctaatgca aaaacaaata
     8941 ttactgtaaa cggagcgtcc aaagtttaca aatatcatgt tgatgccaac aaaggtggtt
     9001 ttacagacga ctacgtagga ttagtggagg gaaacaaaaa tatacgctat ccttctttcc
     9061 aaaaggaata cggtgaaaca aaccttgttt tagaaggcgt aacaaccaac accaaagtca
     9121 atattgacta tggaaaaatc ggtacgtata acgggaaaaa agtcaatatc aagctagttc
     9181 tatctaatat tcacttatac tcggatacgt tgccgtggaa tctactagat aacaactata
     9241 caaaaactca ttttcgagac gatggctata aaaatacgaa tggtgcaatg agtaaatcta
     9301 aaaaaagaac agtactttgg atctcagaca atcttttctc aggaattgtt taccattcaa
     9361 cacaaatgaa tgtgcagcta gtagccacat atgaggacgg tagccccgtt caattttcag
     9421 gagatacatt tatcagtttt aactctttga accctgcagg aggaaaaagc acggatctta
     9481 aaggagaata tgcccactat gacaaaatga atacgacaga ttggtatgtc agacaagatg
     9541 cggttctttc agaatttaaa tctttctaca acaatctgaa cgtggtaggt ggccacccag
     9601 gaggaagctc taagctgaca caagcggata atgatttcaa taatttgcat gataaattag
     9661 gagatccgaa gtttggtcaa ggtactgtat cctttaaaat atctgaagca aatccgacgt
     9721 ttgtgattgg ttcttctaat gttcaaacct ggtttacttt atcttctgcg acaatcttta
     9781 gtgttgttcc tgatcaacct gaaaaaacag gggttgataa aaatggaaat aacgtgaacg
     9841 ataaaatgtt gcaagtagga gacacgattc aatatcgaat caaacagaag gtgaatcgat
     9901 taggagtgga tttactagcg gtttacgatc gatttgagtt aatcgataat ttaccaaaag
     9961 aagtagatta cgtcgatgca cacgttgaaa gtggtacaaa taaaaaattc gatgtatctg
    10021 gtgaagtgac ctatgacaaa acaaaacacc aggtaaaata tgcagcgaaa gctgatacat
    10081 taaaaaatcg aatgaagtat aacggcgaaa cttacgaatt agtgatcaat gtaaaagtca
    10141 atgaattggc caatcaaaat agtgtagcca aaaatcaggg aacgtcgatc attaataaag
    10201 tagaaaagga tacaaatatc attaccgttt atttccctaa aattccagtg aaagaagtac
    10261 aacaaaatgg aaaagatgta aacggacgaa atgacggtaa gaagggtacg ccgacagctc
    10321 ctttaaacgc aggatctgaa gtacaatatc tagtcacaca aaaatggcac acaaaaggcg
    10381 tagacgctgc gtcagatcac tacaaacaat ttagtattca agatccaatt gaagctcgtt
    10441 tgacatataa agaaggttct gcgcaagtaa tagataagag taaaggaaaa gatattactt
    10501 ctgaaggcac actaacctat gatagcaatt caagaacgct gaagtgggaa gcttccgctg
    10561 acttcttaaa taacaatctt ttagatggac gagaaattca gctgatattt acagctaaaa
    10621 ctccattaca atcagaaaaa aatattgata accaagccgt agtagcagta gataatgttt
    10681 ctaataaaac aaatgttgtg acaattgggg ttgatcctaa cttgccacaa gttattgttc
    10741 ctaaaacagg ttctacacat ttagtaacga ttttagcggt cagcttacta ttacttgtgt
    10801 tagcaacttt tggctacgca gttctgaagt tcaattaaac aaaaaaacaa ggctgaaaat
    10861 cagccttgtt tttctatttg aaaatgggat tggcactaat aaatacgttg tctgaagaaa
    10921 gtgatccgtc tgcagaaact tcataagcat gggataaacc aacgatactt tgattgttgt
    10981 aaagcaagtt tgcgttatga gcagcgctat ctttccacat aaccatagca tttactgctc
    11041 ctgtatctga aaggatctcg tcaacgtaaa ggtagaaact attgatattt tctcctttag
    11101 caccgtagca gcctaagctt tctaagagat cttggaaagt accgccgcca ggtcggtaat
    11161 gactgaagct tacttgtaat tcttttgcac gaatatctgc agcttgttga acgatatcat
    11221 tgaattgcat tggggcaacg ccattttgtt gacggtagct atttaaccaa gcaagttctt
    11281 tttgacgcac atagttgttt aaatcactta gtgagatatt tttgtatttg aaagtgatcg
    11341 ttcgattttg gtcaacatta ttgattacac gttgtccatt tttatcaacg gtaacagatg
    11401 aatcgtcatt tctacacaag aagtagtttt gatcggtcat atttttccaa gaagtagaga
    11461 aactatctcc ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt gtgtcaacgt
    11521 ttatatggtt cacagtgacg ttgtattgat gagtataggc aaaattgatt ccttgattac
    11581 ttgtaacgtt gtttaaagtc acagtgttgc tagagctttc tctaggatca agaacaaaat
    11641 tagtgaacat aggtgcttca attgtgatgc tttgtccata aacgacggga taactcttac
    11701 tgtaattata gagttcttgg atatttggat cagtatccga gactttattt ggacgagctt
    11761 tcacttcaac tagaaagact tcatcatagt aaaaagtaaa tgtggtattt gtgttaatcg
    11821 tttgtgtttg tgtttcattc ccatttactt gatactgtgg tgcttcaaag ttagtcaagt
    11881 cggttctagc ttttgcagtg taacttgtac cagcttttac tgagacatgt tcttctttta
    11941 gcagcgttcc agtttgtcga ttcatatgac ggacagttac cgcatattct gaaggtttag
    12001 gtttgtgttg taacccttta aatgcagaat caagactatt tttagcatta tttacttggg
    12061 tttgagaagc tttagaatcg tctagtactt ttttagcatt ggataaagca gtctgaaaat
    12121 tattccaaga tccttctgta aaatctcctt tagcagttga ttttacttgg ttgtacaagg
    12181 cttgtaattc cgttttgttc acagtaggag ctggtttttt ctttaatccc ttgtaagcag
    12241 aatctaatgt gtttttggca ctatccactt gtttttgagt cgcttttgag tcatttaaca
    12301 cttttttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg gtatagtcac
    12361 cttttttagt tcccttaacg ctcgtataaa gagcttctaa agctgattta ttcactttgg
    12421 gtttagaggt gtcagcacca taccaaccaa taccttcgta tttccagcca tagtctttgg
    12481 ttaaccattg tacttctttc caacttgcag tgtagtgatg cgaaccagag gtaacacctg
    12541 gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccat gcaggccctt
    12601 cataagtcca accgtaattt ttggtcaacc atttcacttc atcccaacta gctgtgtaat
    12661 ggtgatcacg aaggccttta ttgtataaac gatagacaag gtggccaccg ctagtcggtg
    12721 cttcccaagc ggggccttca taattacccc acttaatttt cactaggtga tcacgttctt
    12781 ttgtgctaga ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga tacatgacgt
    12841 tagttgaagc agcgtcagca gatccagcaa gagcaactgt ggcagcaaat gttccaatag
    12901 ctataacagt ttttgggttt aataatttca tgttttcaac tcctattaaa tgattaaata
    12961 ttttgtttac attcataata taccacggta taccgaggta gtcaacggta taccgtggaa
    13021 attttgaaat aaaaaagccg ccctatgaag agtgtaagga tggctgttta tatgctatga
    13081 agaaatggat tcggcgtatt tggttagttt ggcagcatta tcaaatcgca ttttttcaat
    13141 cgcacttttt ttctttatta aatccgcaat tgttgattct gcgactgaag aatgtttgct
    13201 aatttcatat gctgtcttgc cagtttcaaa tagtaaccat tcgattttct ctttatcagc
    13261 gtacattgca atgctccctc ttgatttttt ctttattata gctctttttt gtaataaatc
    13321 acaatatcaa gaggaaaagt ttctatagaa aggtgggaaa ttatgagtat ccgttccccg
    13381 tgtatcaaaa tgaggtttgt tgctactttc gttatttgat atactaaaat aaaacaacga
    13441 aagaaggaat agaatgaaaa agttaataat ggtatttact tcgttgattg ccatgattgt
    13501 tattttaggt ggatgtggta gtgaaaaaaa taagcaagat agtcaagata atggcgtgtt
    13561 aaaaccagga acagtgtgga aagaagaagt aggcggactt gttcataatt taaaaattat
    13621 agatgataca acatgggaat attccgaaag tgtttggcat cctgaacaag tacaaataac
    13681 agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg attcaggtgg
    13741 agtagattta ttcataggta aaacagcctt aatagttccc tataacaaag acggtttaga
    13801 aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg aaatattaaa
    13861 agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat tgctagtctt
    13921 tttatttatc ctgaaggagg aatcacagtg gcaaaagcta aaacgccatt tgaaaaagcc
    13981 gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt
    14041 gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg atatatctat
    14101 caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca
    14161 attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg
    14221 ttgatatttt tagagcatta caaactgcag caactagctt aaaaaacaat ttaatgattt
    14281 ttgccggcgt tgcggccgtt gcttccattg ttattggggg gcttttcttc ttatttggac
    14341 gaagaggtgc tgaaaacggg aagatgcatg tgggatatgt attgatgggg gtaggaatta
    14401 ttttagctgc cgcttctatt gtggtttcgt tatttggaat gtttggacaa accccacagg
    14461 gattataaaa acaaaatgaa ggagtaaaaa gtacatagct actttttact ccttttttta
    14521 tgaaaggaga atattcatgt tttttacgat ttcaggcaat gcaaaattgg ataaagagtt
    14581 aataagaaaa gcaacgaaga aagtgggaaa gcgtacttcc tttttaccga aaacaattaa
    14641 atattctgtc attgaaacgg aagaacagct acatgtgtta ggacaagtgt tagaacaaga
    14701 gaatcaatct tctgttaaat tgactctcac gttacatgat aaagaaggaa aaatagaaaa
    14761 ggctggcaca gaatacgttt ttggcccact ggattatcaa atttcagaag gtttttatcc
    14821 tacattgatg gatttaatcc gccttgatgt gtttaaagaa acggatgaaa ctatgagtgt
    14881 ggaagagtct tatcgaaacg aaaaactttt acaagtttta gaagaagagt tagagagcga
    14941 ccaaagaaaa gtgaatcaag tgagtgaagt cgaagtaaat gaagaagtac catgggaacg
    15001 accaatattt ccaaagaagg agtcaaaaga aaaagagaca gtctatgttg acgaacaagc
    15061 tgatattccg actcagaaat cgatatttga ggaagagaac gatgtgatgg atcaattgga
    15121 ggtagatccc tttgaagaaa caatggagga tgaagagacg gccgaagtag aagttgatcc
    15181 agttgaagct aatgaagagt caacatggac tgaaggggat tctcaggaat atattgaacc
    15241 acttatcagt gaagaagtag aaaaaaacga aacagagata caaaaggcaa ctgtatttga
    15301 ttatattttg gatcaatatc cgccagagca tgaatggatg aaagaaaaaa acaaattatt
    15361 tttaaatgag ctttattcga aatatcaatt accccaaacc tatcaagcat ttttagagag
    15421 taaagaagaa ttaatctcac agggtaaagg cacactttta gaacagttag agtctgtcca
    15481 atcaacaaat tggcatgagc ttgcacaaga agatttatta gccacctttg aagaacgtca
    15541 acaagaaaca gatcaagaaa ttaatgagta tgcgacggaa cagtcgtcta attgggagaa
    15601 agtaaaagag gaactaaata aagaagaaca gcgagtgatt gaagaagaag tacaaaaaat
    15661 caaagagcgg tacgaaaaga aacgcgacaa tgcttattcc ttgtgtgtag aaaagatcaa
    15721 taattttact aagacgaatc aagaagcgtt atcgaaagaa aaagaaaact tgttaaaaga
    15781 acgtattaaa gaacgaaaag aagagttcta ccgcggttta cgtacagata agctaaagat
    15841 tagcaaagaa ttaaatgaac aactgaataa gctatatcaa acaacgactg aagttcttat
    15901 ggaaaaacac gaacagatcc agcaagaatt acaagtacaa attccaaaat ggaaaaaaga
    15961 gtatgaaatt caacaacaag aattgaaaga acaagaagaa aaacaacggc aaaaagaaaa
    16021 agagcgtgaa caattagaaa taagaaaacg ggaacaggat cttgcggaac aaaaaatggc
    16081 gatagaaaaa caacggttag agcaagaaaa ggaaaaagaa gcagctgctc gtaaggaacg
    16141 tcaagaacaa atggatacat tgcgacaagc tcagttaaat tatctagcac aacctcaacc
    16201 aacgccttca gtagctgctg ttcaaacccc aatgaacaac caaacaaatc taactccttc
    16261 accacagcgt tcctttaacg gatggatggt gggatgtatt gcaagtttaa gcttgcttct
    16321 aggtggagga acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt
    16381 ggccacaacg acgtcaatca aggaagaagc ccaagaacaa gcaagaaaag agtatgaaca
    16441 acaattagaa gaattattga atcgtaccaa tcagtcaaca acagaatcaa gtatggaaaa
    16501 tagcaaagag agtgatccta gtaaggctaa agaaggatca agcgattcaa ctgtacaatc
    16561 aaacacttcc gaaagttcag aatcaaatga ggtgaaataa tgcgtgttag agaaacaacg
    16621 agacgaaaag acaaacgatt caatttagaa aagaataggt atctttctca tttctatcgc
    16681 tatactcctt caggagaaga gctaccagaa aaacatgaca aacgtatttc tcaaaagatt
    16741 ttttatagtg gcttagctta tcttccttta aactatgttg ttacaagaat accagaagca
    16801 tttcatcgat ttgagattat tccaggaagt ttacaaggag tgaatatttt tcagttacaa
    16861 gaaatgccgc agttattcca ttttgattca actggttttt ggaatccttt gaccccaaca
    16921 acaagtttag gcacacgttt agttgtttct acgatttgtg ttgttccagc tgtacttatg
    16981 ggattgaaaa tggacatgtg gtttcgtcct ctagcagatg ggcaaaaagg agataaccga
    17041 ttaaaaacgt taaaggaaat ccaacaaatt tatccgcaaa taccagaatc aaagggaggt
    17101 tttcctggtt atggcggaat accaattact cattataaaa agtactggta tattcagacg
    17161 gatacagtaa atacttgtat tgttggtact tcccgttcag ggaaaggaca gatagaagtt
    17221 ttagcaacca ttgacaattt gtctcgtgca gaaagacaaa gttcaatggt tgttaatgat
    17281 ccaaaaacag aattatatgt tgctagtaaa gatgaactag aagctagagg gtatgatgtg
    17341 tatgcattta atatcaccga tccactgcaa tcaatgagtg ataatccatt ggcactcatc
    17401 gtgaaatact ggaagcgagg agacatagac acagcgacac agttaaccaa tactttcacg
    17461 aatacgattt attatgatgc caacgcttca gacaataaa
//
LOCUS       DAFFFQ010000058        17125 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000058
VERSION     DAFFFQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.058"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..185)
                     /locus_tag="LQB69_002026"
     CDS             complement(<1..185)
                     /locus_tag="LQB69_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526175.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGNI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(322..657)
                     /locus_tag="LQB69_002027"
     CDS             complement(322..657)
                     /locus_tag="LQB69_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526176.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGNLVPYYTTWEMNEEGINNFPCKSI
                     KESISLTEC"
     gene            complement(678..2270)
                     /locus_tag="LQB69_002028"
     CDS             complement(678..2270)
                     /locus_tag="LQB69_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526177.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2289..2711)
                     /locus_tag="LQB69_002029"
     CDS             complement(2289..2711)
                     /locus_tag="LQB69_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526178.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            complement(2713..3009)
                     /locus_tag="LQB69_002030"
     CDS             complement(2713..3009)
                     /locus_tag="LQB69_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526179.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            complement(3478..3708)
                     /locus_tag="LQB69_002031"
     CDS             complement(3478..3708)
                     /locus_tag="LQB69_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526180.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTAERK"
     gene            complement(3730..4536)
                     /locus_tag="LQB69_002032"
     CDS             complement(3730..4536)
                     /locus_tag="LQB69_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526181.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            complement(4547..5191)
                     /locus_tag="LQB69_002033"
     CDS             complement(4547..5191)
                     /locus_tag="LQB69_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526182.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQANIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDNHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVVDFSYFTR"
     gene            complement(5207..6409)
                     /locus_tag="LQB69_002034"
     CDS             complement(5207..6409)
                     /locus_tag="LQB69_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL3526183.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPL
                     PFLNSSKK"
     gene            complement(6483..6818)
                     /locus_tag="LQB69_002035"
     CDS             complement(6483..6818)
                     /locus_tag="LQB69_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3526184.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            complement(6818..7081)
                     /locus_tag="LQB69_002036"
     CDS             complement(6818..7081)
                     /locus_tag="LQB69_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HBL3526185.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            complement(7140..7517)
                     /locus_tag="LQB69_002037"
                     /pseudo
     CDS             complement(7140..7517)
                     /locus_tag="LQB69_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            complement(7648..9525)
                     /gene="ltrA"
                     /locus_tag="LQB69_002038"
     CDS             complement(7648..9525)
                     /gene="ltrA"
                     /locus_tag="LQB69_002038"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3526186.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            complement(10213..11907)
                     /locus_tag="LQB69_002039"
     CDS             complement(10213..11907)
                     /locus_tag="LQB69_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL3526187.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            complement(12070..12663)
                     /locus_tag="LQB69_002040"
     CDS             complement(12070..12663)
                     /locus_tag="LQB69_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526188.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(12664..12996)
                     /locus_tag="LQB69_002041"
     CDS             complement(12664..12996)
                     /locus_tag="LQB69_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526189.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(13046..15418)
                     /locus_tag="LQB69_002042"
     CDS             complement(13046..15418)
                     /locus_tag="LQB69_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526190.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEIKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQ
                     FKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQNIVTREAEP
                     KPIQNVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLRDIKQDIKP"
     gene            complement(15415..>17125)
                     /locus_tag="LQB69_002043"
     CDS             complement(15415..>17125)
                     /locus_tag="LQB69_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBL3526191.1"
                     /translation="YFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGG
                     FNYQDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPF
                     TLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGY
                     RGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIVYRKKRGETHL
                     PELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVSIFLSQLYK
                     ELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQSYQQL
                     YAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHI
                     NRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQY
                     IEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMHDQIEEEAKEN
                     IFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIRKRLLLEAEKA
                     VKE"
BASE COUNT         4580 a         3539 c         2643 g         6363 t
ORIGIN      
        1 ggataatctt ctgctgcatc aacgaacgtc ttcgtagtca gatttaacgt taaaatattg
       61 ccactttgat aagcttttgc cgctttgtct gtgacgacaa aagctctgcc cgtttgaatt
      121 tctgtcacaa aaacgagatt atctctgctt ttttcaacaa caactccaat cgttgaatga
      181 accatttctt cttcctccct tctctttctt ttgatataat gtagtcgcaa atcgcaccac
      241 tcaatgaatt ggcggaaaac aattcattga aaaaaagaat cctggacaat cacgttcagg
      301 actctttttt tcacttaata actaacactc tgtgagtgaa atagactctt ttatgctttt
      361 acatggaaag ttattgattc cctcttcatt catttcccaa gtagtataat aaggaactaa
      421 attcccttca tattcttgaa tgataaaata tttttcaccc ttcacatgat tacaacaata
      481 cacaccagta gctgggtact caacgactaa aaattgattt ggatcaaact gttcgtttct
      541 caacatttct cttatctgga acaaatgctc tttttctaaa caatcaggaa gttgttcgat
      601 tcgttcttct cttaatgatc cttctataac tttccattga tctaaatatt gtttcattgc
      661 tattcctcct actgtcttta ctgtctttct tgttccattt gaattcttcg ttgttgctct
      721 tcgtatgctt tacttgcgtg gtatttttct tcatgggcat taatcgttcg atttaattga
      781 cgcaaactcc gctgcgtgac aactctttgt tggaattgtt tcctgcgctt catgaattct
      841 tcgtaactat atttgtattc attcgttggc aattgtttaa gcgttcgaaa ggatcgttca
      901 ttgtaaatag attgcatctg atcaacactt tctattaatg gttttatttg actatcctgt
      961 ctcttcagct ctgttaggat ggcatttcct gtgcgctttt ttaaatcaaa gagagcattt
     1021 ttctctgcat cctttgattc tctcttgctt tccccataaa gactttttgc ttcttctttc
     1081 aatgcttttg aaatctgtat atattcttga tattcaggtt cttttgttaa aacatatttg
     1141 gttaactcat cgagtttctc tcttgtgtat ggtgaaagtt tggttcgttt ttcatccccg
     1201 taattccatt ccgaacgttt gtcaggtagg gttttatata cttcatttaa taagttcatc
     1261 gcgtgtcgat cgtaacgcgc tggctctttt accacttttg ttaaggtgtt acgcaaatca
     1321 cttttccgcg tcaataaatt tcgtttctcc ataccaatca ttcgatcctt ttcataagaa
     1381 aaacgttcta actcatgtac ataattggtc ttcattgcgt ctaatgttgc ttgtcttctc
     1441 ttacctttcg gtacgatata actcacacca ttttcaacct ccgtgtaagg tttacgcgta
     1501 ttttcttttt caacaactgc aaaatgaata tggatatgtt tagtgttacg gtgaatcgtt
     1561 gccatccagt agcctgtttc gttcagttgc tctttctcga ataatgtctc catcattttt
     1621 cttccagctg cttttaaccc ttcttcatct aaagtatctg ttttgggatc gtacaatcct
     1681 attttttcaa gatacgaatt atcatgagaa acaacatctt gataaatgat tgagcctttt
     1741 tgttgtcctc tttggaagat ttgcttaatc tccttcagat cacttccttt cacttcgtct
     1801 gaagctatag aaaaaactcc aggaagattt cctgaagctt ttttatgggg ttctgtttga
     1861 tattttttca ttgcttggtt caagcgttgg tattcttttt gttcactttg cgtcagttct
     1921 ttcttttgtt ctaacgcata gtgacgtgtc atataagaaa gataattggt aaaatcttgt
     1981 tcctctaact tgtcaaaatt gattccatgt tccatcaacg attgagcttc attcgacgtt
     2041 tcactgaatt ctttacccgt cttactctcc gaaaaagttt ctccttcagg aatataaaac
     2101 tcctctaaag cattcttgat tcgttgaagt tcctgttctt cttcgggcgt acgtcgttcc
     2161 ttttcttcaa gagcctgttc ccttgtaata tagtcaatat aagtagaaaa tgacttttta
     2221 ttcggcaaag taaattgaca agtgagaata actgaaggcg attgagtcat tattatcctc
     2281 cttcattttt atctttttag aatcttcgtt ggatcaaatg gatctactct ggcatgaaca
     2341 gattcattca caggtttctt caactgaggg gaatattttt ttgtttgatt ggatttaatt
     2401 tttccttcca ctcgttcttt tgcttcttga taaacagttg aatttttacc aattaatatt
     2461 cctgtatctt tcaaatgaag atccgcaaat tctgaaacca tttcggttaa aatagaaatt
     2521 tctttactca ttccattcaa ctttgcttga ttggaattta cttttttctc cacttctgga
     2581 tcatagttca tacataaaaa gcgaacagct tcactatagt ttttaacctc tgcatgagta
     2641 gaaataattt catccaatac atcgatatgt gtttggtgaa agtaaatgtg tttgtctata
     2701 acctttgcca tatcaaagtt cacttcctct attttgctca atcaagattt ctataatacg
     2761 attattttgg ctaacatatt cttctaatat ctttgtgtta taactttgaa tttctgaaaa
     2821 cttttctaat aactggtcaa acttttcttg tttgtattcc atcaaaggac gatcaaaatc
     2881 attttcaacg agtagcttta aataatcatt acgactccat ttatttcctg tacgattact
     2941 tagttcaacg catcttcgat caatttcaga aatgaatttt ggatctaaat ctctaatttt
     3001 aacattcata aatatcctcc aatataatat aggatagtga aaaggatcct atccttttcg
     3061 ctatcctacc tctatccttt tactatcctt tgctatcctt gtactatcct atatattggt
     3121 attttggggt catttatttt tttcagaaac gtgataggca cagcctatcc aaccactttt
     3181 ttttaaaagt ggggtcacgt ttccttatgc tctttaaaaa ttaaggttat ttgtgtattt
     3241 agacaataaa aaatccttct aactttgtcg attagaagga aatattttgt catatgaaaa
     3301 atgaatttaa gaaaaagtgt atgctataat tgatacgctg tcttcctgtt tggttgaaat
     3361 cttcagccag aaaggaggag tgttaaatgt tttatgtgct tgatgtttgt gttacggtga
     3421 tatagctgat ccataaacat ttttgatata cttttttcac ataaaatcct cctgttttta
     3481 tttcctttca gccgtaaaat attttttagc agctacatat agagcttcaa acgatctgaa
     3541 ttttaaagta tctaaattct ttcgaccatt cttcaactca gatattgttg tgttagataa
     3601 attcacttct cgacatattt ctttagcttt tacaactttg agataggctt taatcaatac
     3661 tttttgctca gcgaaagtat ctttgtcagc caagtttgtt cgcttcattt tttcactcca
     3721 cctgtccttc taaatctttt cttttgtcat acgatcaatt ttttcttgaa gtttttgaat
     3781 gatctcttct tgatctttca gttgcgtttt cgatgtttca atttcttgct ggtagctatt
     3841 aatcttgttt tcagtatttt cctgatcatc tgcgacttga taaggcaatt gatcttttag
     3901 cttcttcact aattcttcat tcaaatcaat ggttgcttgg aaagattcca tttgtttttg
     3961 agctgctttt tgttctttct tgtatccttt cactttgtaa ttgatcgcgt gttgttcata
     4021 atcttcatct ttcacttgat ctttaacctt atcttcgtgc acgtaataaa tcagatcttg
     4081 tggttcttgc atatccactt ttggatcaat tttttcagga ataatggtta aacgcatggc
     4141 gatatattct tctggtacat gcgttagctt cactgcaaaa taatcatcag acaccttgat
     4201 cactttggct cgtattgctt gataatcccc tttagctgtc tttacttcta cgcgataatt
     4261 aagattagcc agttctttta acaagctttg atcttcatcg tcgatcgcaa attcaaaaac
     4321 taattgctgg ttcgctcctt tttcttttga aaccattgtc agctctttcg tgtttaaaaa
     4381 ggcgttcgtg cttacctcat ctggtatatc taccttctga ttcgtataaa tcggactagc
     4441 aagaatcaaa aagccgagaa tcataaagat tcccagccat tgaagataac tttttcgttt
     4501 tttgaaacgt tgtctttctc cttttttctt actcattttc ttcctcctaa cgagtgaaat
     4561 aactaaagtc tacgactttc cattgaccat ctacttgttt ttcagtgaca gttaatccac
     4621 cttttatttt ttgtggtttg acatctttga attgcggatt gccgattgta taatcaacaa
     4681 atacgacaaa attttgttcc tcttttacac tgcttgtttg agcgtaagtt tgcacgtcac
     4741 ctaatgtaaa ggagtaactt ccgtaatcat catgttggtg attatcgtct gcaggactta
     4801 atttgtcgat cacttcttgg gttgcgacat cttttagttt ttccgttaat tcatttctgc
     4861 ttaatgattt atcaatggcc aattgaccga actcttctgc cgtctttctt tgtgcttcag
     4921 ttacttcctc ttttgctttt gtgccatctt gttgcttcag ctgatcgatt tcttggtttt
     4981 tctcattgat ggtttgattt aacttgtcga tgttcgcctg tgcctcagag gaattttttt
     5041 tgagcttctc agacgcgttt ttagccactt cttcaatttt ataggtattt gctgcttgtt
     5101 cctctttcag ctgccaaaac aacgcaatat tgccgattaa actaacgcta attgcagctg
     5161 ttaagacgat ttttaacgtt ttattttcca tattttttct cctgatctat tttttactgc
     5221 tattcaaaaa tggtaacgga tcgatccatg taccatcatc aatgaatgca tgtccttgtg
     5281 cttgacgcca atccattcga gtaaatccta aatgcaagtg tgctgtatct cgtatcccaa
     5341 ttacttgacc aactttcact tgatcgccta ctttcactcg agaatttcct gtactatttg
     5401 caaattcttg atacaccata ttcaagccat cgtaattaat cacgatcaca cacgcgccca
     5461 aacctgaaat accaggattg ccaacgtaca cgacttttcc gccatgaacc gcatgaatct
     5521 cacttcctgg atgatcaact gaaccaaaat ctaatccatc atggaagccg ttcggacgga
     5581 attcaccgcc tggattcgtt ccaaataatt gaccaccgct gaaggaactt ttatctaaac
     5641 tgctgccagg gaatggattc gtccattcac cagtaattgt gccattgtta ttgccacctt
     5701 caccccaatg tttgatggcg aattcatcat attcggttaa tttattagaa gtgataattg
     5761 acatacaacc tgtataatac ggactatctt cagtaattcc tgcagcggca taaccacctc
     5821 gtcctaagat caataagctt gcgccagggc ttttatttcc tacacatgca ttaaaatttg
     5881 attgtgtggc taccataaag ccgtaatagg agaatgcagc ttttgagttg ggaaacttca
     5941 tgtaccagcc cccttcaatg ccaccaattc ctcgtgcagt tcctttagga aacagacaac
     6001 catcaaacca agtaatccca aaatagttga gatcatttct tgcggacgcc gacgtaccaa
     6061 acgctgattc ataaccgtat tgcgaaagaa tcacacttgg taataattta tatttcactg
     6121 ctgcacctac ggcatattgc gcaatctcat cagaaaaagg gcctcttcct ttgatttctt
     6181 tataaatcgg taagccttcc gaatactctc ctgtaaaagc ttcgccacca ctacttgaat
     6241 cggaatccga attatcaatc gaacccgccg caagtattag aaaaagggtt acgaacacag
     6301 tgatcaaaat agtaatcaaa cctgcagaag taccaaacaa ccacaaaaat aagcgtcgct
     6361 ttgcacgacg cttcaaatgt tccataacta tatttgtatc attttccata tagtcagtac
     6421 tcctaacttt ttaattttaa acaacaaaaa agcgaagaaa taataattat tcttcgctgc
     6481 ttttaataaa ataatttaat tttttcaata cattcttcta aaatttcctt tggcacagct
     6541 tccacaaact gaaaacctct actctcaatg tcaatcattc gatggtgttc caaaagaatt
     6601 tgtcctttta ttttttgcgt accattcaaa tcaacatgta caggaaaagg cttcgctttt
     6661 gttgttatcg gtgcgacacc aatcatatag ttcaaatatt tattaggttt agataaaata
     6721 atacatggtc gttttccccg ttgttcatgt cctttggtag gatttaaatc aatataaaca
     6781 agatctcctt gcgataaatc atgatagttc tttttcatta aatgatctcc ctaccttgca
     6841 tatttcccca ttcatatttg tcttcttttc ctaagggttc tccttcataa ttggcgaaca
     6901 attcatctaa agtttctggc tttttatttt taggagttaa aacaattttt ccttctgtta
     6961 ccaccatttc gaattttaaa ttttcagtat tagtttctcc tagaactgac aattcttctt
     7021 ttccaaaacg aataccttgt gaatttcccc attttttcat ttcgatttct tttactaaca
     7081 tggaatccct ccttctctca cacattagta tatacttgta tacaccaaaa tacaagaagt
     7141 tactttccac catcaaacaa tttcaattga cgtttgttcg gactaaaatg aacacgaatg
     7201 gtttcttttg agccaaaatt gatcacagcg tctccttgat tttgttgagg aatggattct
     7261 agttctgagt tcgtcattga tttaccaact aatccttcta tatgacgtaa aatagattca
     7321 tccatacgca taaagatctt cgtagaagaa aattcaaaga ctttcttcag tttcgagaag
     7381 ttatcagaag acatattttc tggcaccatt tcttgtgggg attgtgtagc taaaacagtc
     7441 attgcttcta ctttacgcat ttctcgttga aagtttgtga cataatttac cgtttcaata
     7501 aaatctggat taatcgatac tgataggtaa gagtttgata ttatctccgt cttttccccc
     7561 agtccacacc gtacgtgcga ctttcaccgc atacggcgtt ccctcgtcta taaaagacat
     7621 tctgttttcc cgtcctacaa atatttatta agatatttct tcgttaccaa cttttaatct
     7681 caactcattg agtttttctt tttgttttgg atttaatttt agatatttta gaagttcctc
     7741 aatcgtttca ctttgtgtag cgtgtattac ccgatgaacc tcctcagtaa caatcactag
     7801 gttttcatag ctatcacttt tgctttcatg ataagggata atatgatgac aatgcatttc
     7861 agataagata agctcctcac ctgttactgc acatcttccc ttttgagcga tgaattttga
     7921 aattcgattg tccgcaaatt caatcgttgc tctcttacta agcactggat tgctacacaa
     7981 ccacgatatc ttccattctt ctacggtttg ttgcttctta tgaatggctt ccctaccttc
     8041 ttcggtgtat ttattaatat ttcttttttt acacatcgga tgtctgtgtt gaatataccc
     8101 tactggtaat attggaaacc ctcttatata tcgtatttgt cctgaccgta ggtaaggctt
     8161 gtatccttcg tactttccgt attgtccttc ttttgagatt ccttgttctc gtagtcgtat
     8221 ttcaaaactt ggtctgagtt ctcttgcaat ttttcgcatt gctacactac aatgagtcgc
     8281 aattttgaag taattatgaa tcccaataac catactgtta tagactccaa tatagataaa
     8341 ggtatctttg ctattaggac ttttttgaat tcgtttgatt tgttttttta gtttcacttt
     8401 aatcatgctt tgtgcttttg tacctatatg cgtattaaca acctttttgt tacctttggt
     8461 cactaatttc atcgtaaagc ctaaaaattc actttcttct tttgaaagat ttgtgatttt
     8521 tgacttttct tcagatattg gtaactttag acgctcctgt aaccacattt tagttgcata
     8581 gaaaactttt ttcgcttcat tataatttct acagaaaatt ttaaaatcat ctgcgtatct
     8641 cactaggtat atttctttta gagttgtact ctttctgagc ttgtgataga tattaccttt
     8701 atcccaagct ccatttttcc taaaatgatg ttttagctct ttggcatctt tttcctccca
     8761 ctgatttgct atccaccagt caaattcgtt gagattgatg ttcgcaagta atgggctaag
     8821 tattccgcct tgtggtgttc cttttgttgg gtatatcaac ttcccatttg gaagtactac
     8881 gggtgctctt aacattttcc taatgattaa gagaagctgt ttgtctcgta tacccataga
     8941 ccagatttgt ctcatgagtt tagtatgatt gacttcgtcg aagaaacctt tgatatctac
     9001 atctaccaca tattccatgt gactttgatt cattcgggcc atacaatctg ctattgcgtg
     9061 ttctgctgat ctatttgggc ggaaaccata gctgtgatta ttgaattttg cttcacatat
     9121 tggttctaat acttgtaaga tgcattgctg cgcgattcta tcccatattg atggaatacc
     9181 caacggtctt aatttaccat tatatttagg aatttctaca cgccttactt tccgagggtt
     9241 ataatgtgcg aatcgcttcc taacaatttc tacgaattca ttacggttga gattttcaat
     9301 atctttaatc gttactccat cttctcctct tgtcacacta ccgtgatttc tcttgatatt
     9361 acgatatgct aggacaatgt tgttatcaga aatgataatc tgcattagat tcttgaaatt
     9421 atctcccttc aaacttttgg agtatatctt atcgaagatt tccgtcatgc cataatattc
     9481 tgcatgccgc aacttcctat cttttggact tgtttctttt gtcatggttt atcacgctcc
     9541 aattggctcg tgaccttttt agtcatacga gaatcccatg gttgaacggg tttcatgact
     9601 tttacagacg aatcgagcct gttcctccag ttccattaca gaaccttcat cagtcgtgac
     9661 tctactttca ccatcactaa agtaagttat ttgtcattca catatttctt actaccactt
     9721 catcgagtat gcactctcca tgttgatggc ttcccctgtt cccttatatc tatccacatg
     9781 ccttaactta gatgctagct tttgccccct aacttctcct acgcctgtaa cgcagcatgg
     9841 tctttcataa caacgacttt tactcaacca caagttagca caatgagtga ttttttgctc
     9901 actgtctctc ccatttctag gagctatgcc tttcggacag tacattcgct agttcgtcag
     9961 tacgtttgta catactcacc atattaagtt tttttggaca cccgacttat cggatacacc
    10021 ctttacctct aaagaacttt cgtggtattc actatttctt ttaccttacg gtatccttca
    10081 gccgacttca ccgagcttca cacaacgttg gtctgcataa actacttgtt gcatgtcgga
    10141 gtatcagtgg aagccctttg tggcgttacc cactcatttg acttctcgtt ataagagtta
    10201 ttacaatagt ttttagacta cttgcaaccc acagcttttc actgcagaac aggtcgcacc
    10261 attatggcac tcatcaatca tcgccaagaa gcgtttgaag tcttgttctt ccatttgttt
    10321 ttctttaagt aaacgtcgtt gtcttcttcc ttgaatcaat gcctgattcc aaatcaacgt
    10381 catagccatg tacatttgac attgttgtac ccctcgatct aagcctgtaa tagagtcaat
    10441 gtcaaaaaag accaactgtt gttggtcaag gtgtcgaatc gtactgtgac catcaaaaat
    10501 catgccatac gtctcacata aggattctac gatggattcc atttcttctt ttcgattctt
    10561 tttctcttgg gaagcacctg gtaattccag ttgtcctaaa aagtctaaaa attctttgaa
    10621 cgtcggataa gcttctggat ctttccctgt gacttgcgtc tcagtatttg aacgcgaatt
    10681 tgaggaagta aagccgcgtg gtactgttcc atactcttca taaaaacgat ttaagtaggt
    10741 cttaaattct gctttcatcg ttccttttag ctgaggactc actaactgta cctgatagac
    10801 tactttgtct aagtgtgttt gataggacgc tgcttcattg atcttcaaat ctttgactga
    10861 tccatcgtca tataaactgg atgcttcatc atacgtccgt gtggcaaata tttctaaagg
    10921 attgatcatc ccgtcagacc cgtctaagcg caccataatg ccgttatatt cctttaagaa
    10981 ggggataaag tctttcccct tttcaaagcc ccaaataaag tctcctgcag ctaaatgggc
    11041 ttccgcgatc attttaagaa cggttgattt accagctccc atcattccta gaagcaaggt
    11101 attagtagac aaccgcacac tatttttata tgtcgcattc cagacaaatg ggccacctgt
    11161 catggtttcc ccatattgag tccctcctgg atcttttagc gtttggtggt ggaacgggaa
    11221 tcctccgcca atatttaacg atggtagtga ttgccctttg atatttccaa accattcagc
    11281 ttgttcatcg aacatggtaa acatcgattg ccaatctctt ttcgttttgt gcagataggt
    11341 cactgcacga taatccatcc cttttaaatt ttcaatcagt tcctgcgctc gttcatctac
    11401 ttcttgaatc gtgtttccgt aaacatgtac tctcactttg acttgcttag aaatttcccc
    11461 attagaagtc aatttacgag catattccgc tagttccagg tattcttctg cgcttttatt
    11521 tcggtcagtc gcttttcgtt cattatctga tcgatcattt aattcatcta aggcacgatt
    11581 gatttgatca atcacttttt gcttttcatc tgtgctaaca tcgagtgtgg ccactgtatg
    11641 aggattgttc atgagattgg ccaaccaaaa aagcgtagga ttttttgtgt agtgataaac
    11701 tgtcacacat cgtgaataac cattaccata cgtcacatat tcttcatcaa aatttaaccc
    11761 accttgtggc atcacttgtt ttaaaaaatc taaatcatac cctttggatt tcaaaatttc
    11821 tatttcccga tttcctagtt gctttaatgg atcaagtact ttttgatcat tttgtggtga
    11881 aaaagctgtt ttaattgctt ctaacatgta cagtcacctc ttcttgtttg attctttact
    11941 tggttaaaag attgcccttt tcccaactta cccaggctta aagtgtgagg aaattttcta
    12001 gtggtctctt ccttcggaac atctttttct aaccatatca atggatattt cattctctct
    12061 tctccattcc tactcatcat tcatattgtt cattcgatac agaatcttga tcaattcttt
    12121 tttgtagagt ggttttaacc ctaaaatgcc actagatcgc attaactgac ggtttctggt
    12181 ttgtaaatcc ttgattgtct ttccttcaat gacgaaaaag aagcttaagt caggacgttc
    12241 ttgttctgct cgtttcaatt cctggatttc ttcgactaat ttcttttcat atttttttaa
    12301 tcgttgtaga tttcttccta aggattgttc catacgattc ttatctaata actgccgctt
    12361 ttgcacgatt tgttccgtta gatccgtctc tgtagtcaaa ctaactaact taattgttgg
    12421 cacatacgta cgcatgacgg tggtcaactg attcattcgt tccagtttct cttgttcatt
    12481 taatccctcc acgtcagtgg aaacgacacg ataataacgt tgaaaatgat cttctttagt
    12541 aactaagact ccctcttctg tacaggattt ataaggaaat aaatccaaga tagatcgttc
    12601 ttctggtttt ccaaactgca aaatagattt cttatcaact gaattttgtc ctttgactcg
    12661 catttattca tcctccttat acgtaccatg ataatttcgg ttaggatcta acgcatgata
    12721 atggttcttg tccgcaaaaa aaatagactc gataatcgca aaaatttgtc gttgacctgg
    12781 gttattatag ggtcgccaaa caatcactaa cgctatcgca atccataagc cataattcaa
    12841 gagtgcaaaa aagagtggta agtggacaag attattgacc attttcgccc aaaaaaagca
    12901 gccaccaagc gttaatagat ctaaaaagaa aaaagcgaaa aacttccatt ctccactaaa
    12961 tcttggaggg tttttcatgt ttatatattt atccataatt gatttctcct tttcatataa
    13021 agaaaaggcc taacttgtag gccttttatg gtttgatatc ctgtttgata tccctcaata
    13081 gttgttcttt caatgcattg ttcctgtttt tggtatctct tgtcacattt atttttgtcg
    13141 gtgcagtaaa tgccacattt gctggcttgt aacctgtatg aacgttttga ataggttttg
    13201 gttcagcttc tcttgtcaca atattttgaa cggacgcatt ctctgtctca ccactttcag
    13261 ataaattgta agaaacagtt gtcaaattcc cattttcagg caaacgactt gatttcattt
    13321 gaacagtacc tgcaggtgat tgatgaacaa agctttgatc attctctttg aattgattca
    13381 aacgactatc tttcattggt aaattttgtg tcttaggcaa accaattttc gttgattttt
    13441 cagttccaac ttcgatgcca ttatcatttg gcagatcgat ggaatcatca tccgtttgta
    13501 cttcttgatt tcgttttggt aaatctgaaa tgcgcttttc attttcagaa ttaatgttag
    13561 atttataacg atattgttct ggatctgttt ttggtaattg accattttgt ccttcttctg
    13621 aattttcgtt agaatgcaac tcatcttggt gatctttggc tgaaggaatc gctgcacctt
    13681 cttgattttc tttttcggtt gttgtattag cttttactgc tgcattgact tgtccttcac
    13741 catacgcatc ttttagtcct tctttgattt ctgaagcttt ttctttttgt ttttctagtg
    13801 tttcggatgg attcttcaca gcttcagcca attctttagc tctcgtgccg tctaatttct
    13861 cttctaaggt gtctttggct ttttctgtaa gatcttcagc tgttcctttt aacccacgtt
    13921 cattgacgta accaagacct tctgcggctt tagaaacacc atttttcgct ttcccgaaca
    13981 tttcggaaac tgttttctta ctattaggta cagttgtagc tttatcgtct gattgttctt
    14041 ccccgttatc aggggtaaca tcttttcgat taccaaatcc atttttacga atatcattta
    14101 aattgcttaa accactctta ccagcacttg caaggttttt agccccgcta cccacatttt
    14161 tcgcaacatt tcctaaggca aacatgctaa gaagagaatt gctgccgttt ttcagactcg
    14221 tatcaacgcc gaaaattttc gtaactgatt gagagccttt gaatagcgca atggttaata
    14281 caattaaagc gacaacatac aagaatccat tgatctttcc tgccgcatgc aaatcaccga
    14341 tacctgaaag cattaatgta tagattcgta attctaaaat cgtaaatgcc aataacaaga
    14401 aggattgaga aatatcttct atagcacttt tcatccgttg tcctgtctca aagtccgttg
    14461 acgcggcaat cactaaatag aattttttaa ataccagctc aataatgcaa gtaacaatcg
    14521 taaacaagat aaataaatag gctaccgaca aagaagattc accagctaaa ataatacctg
    14581 gactactaat gtatcggcga tagcctgaag tatacacata ttttgctaaa ccttcatcac
    14641 taatttgaac agggatttct ttcccattat cgtctaactc tagtcgatac tgtaatgcct
    14701 ttaaccgatc tttttgtact gtactaatat cacttgattc agacattttc ttagcttgat
    14761 caggtgtaat aatcccattg atattcgctg ttttaaaatt tttcgccgtg agagcatttc
    14821 ttttgtctgt tggtatgtgt tctactttgt tcggatcatt ttctagtaac gtactcacat
    14881 caatcacatt attttgaata atttgaaaag caggtgaacc agttttatct gctttatttg
    14941 ctgcagtcac gtctccgtaa aagtttttac taatatttag cccttcatcg attaaagaac
    15001 taccaccaaa aatcaaaatc atggccataa atagattaac caagatattt ttgatttttg
    15061 gtggatgttt actcataaaa aattttaagc ctaaataaat taatgtgacg acacaaaaaa
    15121 gagccaatac cttactaatc aaactttgaa acagttcatt aacaccagcg gcgttcaata
    15181 agtcttttaa atcaaagctt tgataaattg ttttttctag taaagtattg agattatata
    15241 accctttaat caaaccccat aaaaaagtat tacaaatcca actgaataca tcttgatact
    15301 gcagataatt accataatct tgtaagactt tcgttacggt tttgggatct gcatccccat
    15361 ccccaaataa gcctgaccac cagtcggcaa tgccggaaaa tattcctcca atgatcattc
    15421 tttcaccgcc ttttcagctt ctagcaaaag tcgtttcctt ataacctctg taatttcttc
    15481 attttgacgc aatttttcaa ggtattcttg caaacgatct atttgcttct cttcataaaa
    15541 ttgaataagt aacttttgtg ccgttgagtt gtctaaagat tgaacgattt taataaaaat
    15601 gttttcttta gcttcctctt ctatttgatc gtgcatattg tgataaagtc gacgtgcata
    15661 gatatcaggt agaaaataat cgtaatcaat ttgtaaagcc ttaagatcca agtccgcatg
    15721 tgtacaatgt atgtctatat cattgatatc ccacgatgga ttgaaatctt caatatattg
    15781 ataagcataa ggaagattcg tttccccatg attaaaaata ggatacggcc gtattttatg
    15841 acctttcaaa tcttgtcgtt ttaaaggagc gagtacaatc gtttcacctt ctaaaaaagt
    15901 agaaattctt tcaggagtta agatgcgatc tgaatcaaca ttgcgattga tatgagtatt
    15961 catacctaat gaattgatgg tactgttctc ttgttcaacc gtcttattcc ccactttttt
    16021 ggaaaattct tctatggtat tatcgtctgt cgacataata taaatatgtg tctgacaatt
    16081 ttctttgata atcgctgcgt tttgttcccc atacttcgca taaagttgct gataggattg
    16141 gataaacaaa tcccacaaca tgtttctacc agctgtgact gtcatgattc cttccatatt
    16201 atccattggt ggcatattcc caaattcatc taaaataaaa tgaatcctac ggtgacattt
    16261 atctccttta gtcttaacac attgcgtaga taattcttta tacaactgac taagaaaaat
    16321 actaacaatg atatggttag atggatcata atcaggtgtg agcataaaaa tagccattgg
    16381 tttatcggta tagtgcatcc gaatagactc tactaataag ttacttttat cctcatctaa
    16441 tgttagaacg gcttcttttt ctaattctgg caaatgggtt tctcctcgtt tttttcgata
    16501 aacgatattt ccctcttcgt ctttcaatac tctttgagaa aactcatacc aagcaacata
    16561 ttcttttcct cgatgatcgt attttaagca aacatgatct ccctgttcta aatgacaatc
    16621 aaagttaatt tcacaaaaac ctctatagcc tactttgaca cgttcctcat gaaatttttt
    16681 tccttgatga taaaactcta caacaattcg ctggtttaac agactctcgt gtagtttgag
    16741 atccaaatac ttcggaaaac caatggactt ataatcaatg gttgattgag aagtcatttt
    16801 cgcaattttc gtcagtgtaa atggatttag tcctgtgatc gctgtcgata gaatgttgcc
    16861 tttcgcttta tcagaaccaa tttttgttgc cccatattgt ttttttgcta tgtttccagg
    16921 tggtaactga ttcataaatt catctaaagc attgagctgc ttgaattcat tatcaggatc
    16981 ttggtaatta aaaccaccaa tctcagaaag aagttcgacg agattattcg gtgttaattt
    17041 ggagtaatcc cctgtctgat ccgcttgttc caataacgca aaaataagcg cattgacagc
    17101 tttttgcgca ttttcgttaa agtat
//
LOCUS       DAFFFQ010000059        17107 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000059
VERSION     DAFFFQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.059"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            7..1398
                     /locus_tag="LQB69_002044"
     CDS             7..1398
                     /locus_tag="LQB69_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL3526192.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            1414..2454
                     /locus_tag="LQB69_002045"
     CDS             1414..2454
                     /locus_tag="LQB69_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL3526193.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            2473..3558
                     /locus_tag="LQB69_002046"
     CDS             2473..3558
                     /locus_tag="LQB69_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL3526194.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            3591..4553
                     /locus_tag="LQB69_002047"
     CDS             3591..4553
                     /locus_tag="LQB69_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL3526195.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            4546..5979
                     /locus_tag="LQB69_002048"
     CDS             4546..5979
                     /locus_tag="LQB69_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL3526196.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            5976..7046
                     /locus_tag="LQB69_002049"
     CDS             5976..7046
                     /locus_tag="LQB69_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526197.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            7033..8454
                     /locus_tag="LQB69_002050"
     CDS             7033..8454
                     /locus_tag="LQB69_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL3526198.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            8467..9474
                     /locus_tag="LQB69_002051"
     CDS             8467..9474
                     /locus_tag="LQB69_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL3526199.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            9486..10490
                     /gene="neuB"
                     /locus_tag="LQB69_002052"
     CDS             9486..10490
                     /gene="neuB"
                     /locus_tag="LQB69_002052"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL3526200.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            10487..11635
                     /gene="neuC"
                     /locus_tag="LQB69_002053"
     CDS             10487..11635
                     /gene="neuC"
                     /locus_tag="LQB69_002053"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL3526201.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            11608..12285
                     /locus_tag="LQB69_002054"
     CDS             11608..12285
                     /locus_tag="LQB69_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL3526202.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            12275..13417
                     /locus_tag="LQB69_002055"
     CDS             12275..13417
                     /locus_tag="LQB69_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL3526203.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            13430..14407
                     /locus_tag="LQB69_002056"
     CDS             13430..14407
                     /locus_tag="LQB69_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3526204.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            14407..15315
                     /locus_tag="LQB69_002057"
     CDS             14407..15315
                     /locus_tag="LQB69_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBL3526205.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            15316..16068
                     /locus_tag="LQB69_002058"
     CDS             15316..16068
                     /locus_tag="LQB69_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL3526206.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            16073..17020
                     /locus_tag="LQB69_002059"
     CDS             16073..17020
                     /locus_tag="LQB69_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL3526207.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         6164 a         2190 c         3090 g         5663 t
ORIGIN      
        1 gtatatatga ataaaaatgg agaatggagt gctgcacgac ggatattcat tatattaatg
       61 gatgttattg tatacaatgc agcaatatat agtagtttct tactgaagtt tcgtggagag
      121 atcccattac gaaatttaga aactttccaa cactcagcaa ttattatttc agtgattttt
      181 attgcgttga acattttgtt gggtgcatat gttttttata atcgaatgat cagtgacatc
      241 gtgtttgtca cagtcatcgg tcaggtattg atgacactag ggatcatggt agtgactttt
      301 gcgggccgct ggtttgcttt tccacgtctt gttatcttac tttcatttgt tctcggtacg
      361 cttttcttaa gtatctatcg tattctcgta tataaacttt atttacgcgt aagtcgagat
      421 aaaaaggtcg tcattattgg gttagaaaag gatgtcgcac cagcaatcca aaattttggt
      481 tcaaagaaaa acaacaaaca taaagtcgaa gcagtcattc ttgataacta ttatgcaaac
      541 atcaaaaata tgattaaaga catcgatatt gtctatcttg cttctcatat cgaagaaact
      601 gaaaaaatca aaatctatca attagttact aaaaatgaaa aaaaattgtt tttgaataca
      661 acatttgaaa acttgacatt gatcaatcca aatattatga atttcgaaga tgaaagtatt
      721 attgaaacat ctggatttag aatccaacct gaatatgaac tattcaaacg tttgtttgat
      781 atcatcgttt cacttgtttt attagttatt gcttcaccat ttatgttatt cacagcaatt
      841 ttagtcaaag tgacatcacc gggaccaatc atctataaac aagtgcggat cacaaaaaat
      901 caaaaggaat tttcaatcta taaattccgc tcgatgaccg ccacagccga agcaaaatct
      961 ggaccagttt tagctaagag caatgatgca cgagtaacgc ctgtaggtaa atttattcga
     1021 gctgtccgct ttgatgaatt accacaaatc ttcaatgtgc tgaaaggcga tatgtcgatc
     1081 gtaggaccac gtcctgaacg tcctttcttc gtcgatcagt tcaatgcaga aaacccttat
     1141 tactacttac ggcataatgt tcgtgctggt atcactgggt atgcacaagt ctatggaaaa
     1201 tatgtctctg attacaacag taaattacgt tttgatttac tttatatcaa aaaatattca
     1261 ttgatgcttg atttaaagat cttgctgcaa accatcaaga tcctatttga taaaatgtcc
     1321 tctcaaggac ttgaagaaga cgaaaatgtg atctcattgg cagaaattgc ttttccgaat
     1381 gaaaaaatct ttcgctagtt gggaggcgta aaaatgaaca atccgaaatt cagcataatc
     1441 gtaccagtat acaatgcaga agcaactatt ggtcgaacga ttgatattct aaaagaattg
     1501 gattatgaag agtacgaagt cgttttagta aatgatggat caacagatca aacacaggaa
     1561 atcattcaag aactgattga aaatgatgaa cgttttgaat tgattactac gaccaatcaa
     1621 gggcccggat tagcaagaaa cgaaggaata agacagtcaa aaggagaata tctcttattc
     1681 ttcgatgcag atgacacgcc agtaaaaacg atcttgtcag attatgatca tttgctagaa
     1741 aagagtccaa aagcggatct tgttgttagt tcgtttgttt ttcgtacaat ggacgaggga
     1801 agaatcgttt ccgaaaaaga aaacttagtt acagaatgtc actatctaac tcacgaagag
     1861 tttataaaag acatgtatca gttaatgaat gaacagttga tgtatgttgt atggaacaaa
     1921 tgctatcgta gcgatattct gaaaaataac caaattctat ttaaaactta taatagttgt
     1981 gaggatcgaa tatttaactt acattattat gaatactgtc atcaagtgat catgaaccca
     2041 aaagtagaat atatttatga atttgaaggt gggaagggga tcactaatca ataccgacct
     2101 aacaagttcg aaacatttaa agaattctat tcattagcaa atactgtaac agacgatatt
     2161 aataaagacg gtatggcggc gcttctttta aaagggacaa cttcagttat tttttctatt
     2221 tatagtacct ctgcaagaac ttcaaaagag aagaaagacg aagcaaagca gatattgagt
     2281 gatccagtga tccgagaagc caagcaaatt gctaagacgg attcttcagc aaaaaaaata
     2341 acaaagttgc tttacaatat gccagctcca ctatttttat ctgctgtaaa agttggaagc
     2401 ttcgttgaag tgaaaatgcc agggttaatg gctgctttga agcgagtata ttagttaatg
     2461 gaggaaaata taatgaaaag aattttgcat tttcagggac gaatgggttt aggtggagca
     2521 gagtctttca tgatgaattt atatcgtaaa attgatcgta caaactatca atttgatttt
     2581 ttgatttatg aagattatgc agatgttcaa gactatcatt cagagattga aagattagga
     2641 ggccgtattt ttgttgtacc taatcctaag aaaaatattt ttaaatattt aatagaagtg
     2701 aataagttac taaaaaaaga atctttttct atagttcaca atcaggttta ttttggcggg
     2761 ggaattaatt tatggttagc caaaaagaat ggaatcagac agagaattgc tcatagccat
     2821 gcaactgaag atggaaaatc tcaaaatatt gtaatgaatg tattgcgtaa atttttaacc
     2881 aaattgttgt tacaaaatgc aacagactac cttgccgtta gtcaagaagc tggtgaaagt
     2941 ctgtttcaaa accatccctt tgagatagtt cataatggta ttgatttaga gctttatagt
     3001 aaaaattcag aagctaaggt taataagaga aaagaattgg atatctcaat gagtactttt
     3061 gttgtgggaa acattggacg attagagaaa caaaaaaatc aatcttattt gttagagatt
     3121 tttttagaaa tactaaaaat gaattctgat tcttacttga ttttagtagg tgaagggtca
     3181 cttagaaata aactgaaagc caaagcagta gaattggata tagagaaaag agtaaaattt
     3241 ttaggagaaa gaaacgatat tcccgagtta ttatctgtga tggatgtttt tgtaatgcct
     3301 tctctttatg aaggtctgcc aatggttgga ttagaagcac aagcagcaaa attaaaatta
     3361 gtcttagcag atactatttc caaagataca aagttaacaa ataatgtgga ttttgtatca
     3421 ttagatgaaa caccacaaga atgggcaaaa aaagtaataa aagagccgta tgctaataaa
     3481 ataacacaag aactaaagag ttatgatgtt tcgtacacta ctgaacaaat ggagagtata
     3541 tatagtagag atcattaaaa atgttcaaga tattttagga gtgattattt ttgaaaatat
     3601 gttatttgat attggcatac aatgatattg gcaacttaaa gagattggtc aatcgattga
     3661 acgaaaatgc agtttttatt attcatatag ataaaaaaac tgatatagaa ccatttattg
     3721 aagcatttaa atcatatgat aatgttgaat ttttatgtaa taaaaaccgt gttaaaattt
     3781 attggggtgg tttttccatt gtacaagcag aaatcaattt agtaaagaga gcattacaaa
     3841 atgagaaata tttaaaatat gtattattat ctggtgctga ttatccaatc aaagataatg
     3901 agtatattta taattatttt aaaaagaata gttcagtgga gtttattcgt ggaatagatc
     3961 tagatcaaat taaacataaa gaattttatt ataaacatat agatgtatat caaaaacatg
     4021 attatcctag aattaatagg aataacacaa ctgcatttaa aatttttaga gctataatta
     4081 atcgttgtct gaggatgatt aaacttcccc caaaaattag acaccataaa tttgatttgt
     4141 atcatggttc tcaatggtgg gctttgtcga aagaatgcct aacagaatta atacaaatgt
     4201 atgaacaaaa tcaacaagac tacctgaatt ttaaaatagg tatgtttgcc ccagatgaaa
     4261 aattttttca tacacttttt tttaattcct cgttcaaaaa taaaaatgtt attggaggac
     4321 cagatttacc acttgaattg aaaaatattg aagaaactac tttacaaact tcgaaattag
     4381 caaatattca tattattgat ccttccatga acaaatggtt tgatgaaagg gatatagatc
     4441 aaataaaaga aagtgaaaaa ttatttgtaa gaaaagttaa atcggattac tctgataaac
     4501 tattaaatag aattgataaa gaaattttga aagttggaaa tatagatgaa tagaatagta
     4561 cctgttttag agaagagttt attaagtcga gatgcgtttc gagtaataaa tttaatcatt
     4621 tgtctgcttt attttatgcc tagtactttt aatgtaacta atatcccgat gaaaatagct
     4681 tttgcttggg gtggaattat agttttgtat gaggttttta taaagaaaaa tttttttaaa
     4741 atgcgttttt cttggctact gattggagca atgattagtt ttatcttaac tataatcatt
     4801 aacagagaaa attttttaat tccatctatt tataattttg gatatttgct gattactttg
     4861 atagtaattt ttcctgcaga cttttcacaa agtgaagagg aaaaaaagaa aaaaatggtg
     4921 tattttaata atgttttcat tatcataatt tttttagctg cgctaatatc tatctatcaa
     4981 tttatttttt tgatttctta tcatgtccca acaggaacgc caggattact tgcaagacaa
     5041 ggttttattg aacatcggtt atttggtgtc tatacaagtc caaatgttgg agctatgttt
     5101 ggatatactt ctattatttt aagtttaatt agtgtattaa tttcaaaaat aacaaaaacg
     5161 attcgtgttt tttatatgat gaattttatc gtacaaatat tttatattac actttcttcg
     5221 tctagaggtg cccaggtagt aatttgttct tttttagtat tatttctttt gttgtttgcc
     5281 aattcaaaat ttagaaagag cattagagaa gtactacctg taaaaatcaa atggatggta
     5341 agcatattat tagttctatt ggtatatttt tcttttggta ctaattatgc taaggatttt
     5401 ttagcaacaa ttcctgtatc gattgataga aaagtagaag ttatagagtc aaatgaatca
     5461 gataaaactc cacttccaga aaaagaagta atattacagc attctagtga taactccgaa
     5521 atttcagctg gtcgttttac gatttggaaa gctgcgctta aagttataag accaactttg
     5581 ttatttggtt atggagagac cgatttctat gggaatagcg atgcaaaatt tttgaaaaaa
     5641 gtagatcttg atactacaga tgtgaatgaa ctaaaaagag cacatggaaa catgcataat
     5701 ggatttcttc aagtacttgt atcaagtggt ataattgctt ttatatgtat ctatgctttc
     5761 tatgtgttaa atatctttag tttactcaaa acatttttta gaagtaaagt gaattatgcg
     5821 tttcttggta ttgttttaat ttttatacta tctattttta tagatgaaat ggtggaggct
     5881 catgtacttt tcaataagcg cgatgtcatt tcaattgttt tctggtatta tttgggaagc
     5941 atttcttact tgtatttaaa aaaaggagaa ttaatatgaa gacagctttc attgcttgga
     6001 ctcctttaca tttgattaat atattaaatg tttatcaaaa tttttaccga gaatcaaaag
     6061 gagatttatt tatttattct gaatttgaaa acgctagaag atatagtgag cgtataaaag
     6121 aattagggct atttaatgat gttgtttttg tgaatcaccg gaaaatcggt tcttccttta
     6181 aacgtaaaat taacttgtta actaaccata atcaattttt ctcggatgat ttatccgttt
     6241 atgatgaaat ttttatacag ggggaaaatt atttcacaaa aatacttttc tccaatgttt
     6301 acaaaaaaaa tccagatgta aaactaaatt atatagaaga tggtttgggt acttatttga
     6361 agaagagtat attttcaaca agtaaaaacg ttcaaagagt ttttaaaata ttaaataaaa
     6421 atagcatctt caaaaaaaaa attgacgatt attttgtcta ttgtccggaa ttgttacttt
     6481 atgatgtaaa aaatataaaa aaaattccca aaatagatga aaacagtaaa ttgatgaaaa
     6541 acatacaatc aatctttggg atagaagaag tttttgtcgg agataaatgt atcttttttg
     6601 atcaaccttt attatctgat ggttatggca tagatgaaac agatattttc gctaaagtaa
     6661 atagtatatt ttctggaaaa aaagaaactt taattaagct tcatcctaga gcaaggacaa
     6721 atcgttatgg tgaaaaaaac atattgaatt ccagtttacc gtttgaagtc ttgctacttg
     6781 aaaatgactt ctcttcaaca cttttgatta gtcctatatc tacaatttct ttttcaccat
     6841 ctatgatgtt tgaaaagacg atcaactcta ttatgttggc taaattaatt ttaaatgaat
     6901 atacagcatt atctgaagat aaaaaagaaa cattgacaaa tattgttctt ttctgtgaac
     6961 attataaccg tctatcagaa gaaaaaattt tactcccaga aaactgggca gaattaagaa
     7021 agttggtaga gtatgggatt atttaacaca ctttccaagt caaaattagt taagtccagt
     7081 ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac tattcctatt
     7141 tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac ttcatttgta
     7201 tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa tgcaagtttt
     7261 gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc tacactttcg
     7321 ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc tttttttgat
     7381 atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt tgtaattagt
     7441 tatatccagt cttattttac gattaacttt aaccatttta aatatttgat tttatctttt
     7501 atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt atattttgat
     7561 gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat aggtttagta
     7621 atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca gtggagatac
     7681 gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt tttagcacaa
     7741 ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat atattcctac
     7801 attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa cgcctgggtt
     7861 ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa agttagtaac
     7921 tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc gattgattta
     7981 gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt tccgataacc
     8041 ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt ttatgagaaa
     8101 aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat actattaaac
     8161 atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt atttacgtat
     8221 tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa acaattattt
     8281 gacacaaaaa aaatatgtat cataataatt tttgtatcaa ttttttcttt gatacttcat
     8341 attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat aattagttta
     8401 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
     8461 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
     8521 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
     8581 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
     8641 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
     8701 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
     8761 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
     8821 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
     8881 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
     8941 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
     9001 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
     9061 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
     9121 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
     9181 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
     9241 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
     9301 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
     9361 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     9421 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     9481 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     9541 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     9601 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     9661 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     9721 aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat tttttgtcta
     9781 ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa ggatttaaaa
     9841 ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg tatgggttgc
     9901 cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct gtagattgtt
     9961 tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct agttctgaag
    10021 atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc gttgggtact
    10081 ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt gctaaggtaa
    10141 ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa gcttctttag
    10201 atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata tttttaggtg
    10261 atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca agaaaaagcc
    10321 tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct cttacaataa
    10381 aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc aaaaaagtta
    10441 aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga aaaaaatact
    10501 tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg agaatttact
    10561 acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc ataaatatgg
    10621 atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag ttcaaagttt
    10681 gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga taaatggttt
    10741 cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg atagaggaga
    10801 aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac atattcacgg
    10861 aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga aaatagcatc
    10921 tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg gggaagattc
    10981 ttggagagtt tttcccgtcg gagctcctag aatagatact attttaaata atagattacc
    11041 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
    11101 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
    11161 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
    11221 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
    11281 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
    11341 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
    11401 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
    11461 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
    11521 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
    11581 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
    11641 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
    11701 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
    11761 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
    11821 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
    11881 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
    11941 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
    12001 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
    12061 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
    12121 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
    12181 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
    12241 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
    12301 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
    12361 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
    12421 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
    12481 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
    12541 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
    12601 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
    12661 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
    12721 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
    12781 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
    12841 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
    12901 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
    12961 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
    13021 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
    13081 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
    13141 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
    13201 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
    13261 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
    13321 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
    13381 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
    13441 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
    13501 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
    13561 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
    13621 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
    13681 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
    13741 agtagctaaa aagaaagata gattacttta tctatcttct acatttttga agagaaaaga
    13801 aattcaatat attaagtcta aagtgacgca taataaaaaa atttcttatc aataccatgt
    13861 aggacagtat ttacccgact ggcatccttg ggaagattat cgcaattttt ttgtttcaaa
    13921 caaaaaaact aatgggtgta gagaaatatt cgctattgaa ttaccttgga ttatagatac
    13981 atttgggaaa gttaaatcgt ttactgtaat gacccagaaa ttatccaatc ttgatattga
    14041 ttattcggac agtttcactc ttattattga gcatgaaact ggtatagttg gaactttaag
    14101 tgtagatgtt gtttcaagag tagctaaaag aaatttaact ataattggtg aaaagatttt
    14161 aatagaatgg gaaggtaacc cggattctct attttcatgg aatgaagaaa cttctagtat
    14221 gacagatatt caattgtacg atgaggttat gcatgataat aattattcac atactattat
    14281 tgaagatgca tatttagagg agattaaaga atttttcacg cttttggaaa agaaaatgga
    14341 accccaatat acttttgaaa aagataagta tattattaac ttaattaacg acattgaggt
    14401 aaattaatga tgaaagtaat tttttttggg ttagggtcaa ttggaaagag acatctaaaa
    14461 aatctaataa gctatggaaa aaagaattct attaattttg atataacagc ttatagtagc
    14521 agtggaaaaa aagagtttga agaaataacg tatattcaaa atagagacga tttggataca
    14581 cattatgata tagcttttat tacaaatccg acttttaagc atctggaaac actaaatgta
    14641 attgttgaaa aagttgatta catattttta gaaaaacctg ctttttctga gagtattgat
    14701 cttactcaat ataattatga ttacaaaaat atttatgttg ctgctccact gaggtataaa
    14761 aatatcatgg aagttgttcg taatagtatt caaagtaaaa aagtatattc tgtaagagct
    14821 atttgttcta cttatcttcc tgattggaga aaaggtactg attatagaaa taattattct
    14881 gcctttaaat ctatgggggg aggagtagaa ttagactgta tacatgaatt ggattatatt
    14941 ataaacctat ttggtttccc taaatcagta aaaaaacagt ttggtaaata ttcgaatctt
    15001 gaaattaaca gtaatgattt agcaacatat atattgagat acgatgataa aattatagaa
    15061 attcatttag attattttgg aagagttcct acaagaaaat ttcaattgtt tactttagat
    15121 gaatgtatca atgttgactt attaaataat aaaataagtt ttgaagaaaa cattgttaaa
    15181 gaaatgcctg aatctgctaa tgatatgtat gagaaggagt tgaaatattt ctttgaaaac
    15241 gtagtaacag gcaaagaaaa ctggaatgat ttatatcatg caaacgaagt attgaaaata
    15301 gctgaggaga aataaatgga aattttattt actatttgtg gtagagctgg gtctaagggg
    15361 tttaaaaata aaaatttgaa atatttggca ggaaagcccc tagtccattt cgctttagca
    15421 atgatagata tctatcaaca aaaacatcct gaacataatt gcacgacttg tttgaatacg
    15481 gacagtaatg agttagtaga attgacgact aagttaaatc cgtctgtgaa aattgtagaa
    15541 cgtaaacctg aattagctac agatactgta gggaaaattg aagtgatacg tgattcattc
    15601 ttacaactag aaagagaaga taatatattt gatttaatta tagatttaga tttgacttca
    15661 cctttacgtg aagtttctaa tcttgaagaa ttaatagatg aacatctaaa aaatttagat
    15721 aaagacgtct acttttcagt tgtttctgca agacgtaatc cctattttaa tatggtaaaa
    15781 agaattgaaa atgaagttac tatagttaat aaatcaagat acacatctag gcaacagaca
    15841 cctgaagttt ttgaaatgaa tgcctcaatg tattctttta aacctgaatt tctaaaaaaa
    15901 agtaattata tttttgatgg gcaatgtggg ataataaaaa tgagagatta tctaatttta
    15961 gatattgata gtgaagaaga ttttaaatgg ttagaatttt tgtttcctaa atttttagaa
    16021 gattcttcgg agcttcaaga tatctataat tatttatatc ggaggtaaaa ggatgagctg
    16081 ttatcttatc accggtgggg ctggcttcat tggttctacc ttagccaatc atttaggaga
    16141 agaacatgtg gtaatcgtcg tcgatgactt atcaatggga aaagtagaaa atcttgatat
    16201 gacgaaaaat atcactttta ttgagggaga tgtcgctgag aaatccttga tggaagagat
    16261 catgaaagct aataaatttg attatatctt tcatttagca gcagttgcta gtgtggcaga
    16321 ttcagttgcc cgaccagtag aaacacatcg tgtgaatttt gaaagtgtat taatgatttt
    16381 agaattgatc cgcaagtatc aacctgattt aaaacggatc gtgtttagtt cttcagcggc
    16441 tgtttatggg gatgaaccga ctttaccaaa aaaagaagag tccgtgattc gcccattgac
    16501 gccttatgcg gttgataaat tcgctgcaga acaatatgtg ctagattatt gtcatttata
    16561 tgatgtaccc ggtagcgcag ttcgtttttt caatgtctat ggtccaaatc aaaatccgaa
    16621 ttctccatac agtggtgtga tttctattct cgtagatcgc tataagaaac aaatcgtagg
    16681 agaagcaact tcatttacat tatttggtga tggaagccag tctagagact ttgtttatat
    16741 agatgatgta atccaagcat tacttttagt tgcgaatgag gaagctgcac tggggaaaca
    16801 attcaatgtt ggaacgggta aagcaaccac acttttggaa ttgattcatg caatcaatga
    16861 aattctcggt gtagaattga cacttgaata caaagaagag cgttcgggtg atatccatga
    16921 ctctctagca gatattacta agatccagtc aattggctat caaccgaaat atggtatttt
    16981 aagtggaatg actaattatt tgaaaactga aatcaattaa aacatactaa gataagatta
    17041 gagggtaaat tcaggctctt tgtcaataag gactgatgag ttttgtaaaa tcaaatctgg
    17101 gatagaa
//
LOCUS       DAFFFQ010000060        17085 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000060
VERSION     DAFFFQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.060"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            226..894
                     /locus_tag="LQB69_002060"
     CDS             226..894
                     /locus_tag="LQB69_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL3526208.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="LQB69_002061"
     CDS             complement(1094..1480)
                     /locus_tag="LQB69_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526209.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="LQB69_002062"
     CDS             complement(1452..2834)
                     /locus_tag="LQB69_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3526210.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="LQB69_002063"
     CDS             complement(2990..4177)
                     /locus_tag="LQB69_002063"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL3526211.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="LQB69_002064"
     CDS             complement(4184..5215)
                     /locus_tag="LQB69_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL3526212.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="LQB69_002065"
     CDS             complement(5231..6685)
                     /locus_tag="LQB69_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526213.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6860..7765
                     /locus_tag="LQB69_002066"
     CDS             6860..7765
                     /locus_tag="LQB69_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL3526214.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTTVSWTT"
     gene            7856..8533
                     /locus_tag="LQB69_002067"
     CDS             7856..8533
                     /locus_tag="LQB69_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL3526215.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="LQB69_002068"
     CDS             8530..8700
                     /locus_tag="LQB69_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526216.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="LQB69_002069"
     CDS             8814..9710
                     /locus_tag="LQB69_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3526217.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="LQB69_002070"
     CDS             complement(9765..11135)
                     /locus_tag="LQB69_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3526218.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="LQB69_002071"
     CDS             11373..12119
                     /locus_tag="LQB69_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3526219.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="LQB69_002072"
     CDS             12116..12955
                     /locus_tag="LQB69_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL3526220.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="LQB69_002073"
     CDS             12956..13906
                     /locus_tag="LQB69_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL3526221.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="LQB69_002074"
     CDS             14047..14799
                     /locus_tag="LQB69_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526222.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKNADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="LQB69_002075"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="LQB69_002075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL3526223.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16354..16962
                     /locus_tag="LQB69_002076"
     CDS             16354..16962
                     /locus_tag="LQB69_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3526224.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5586 a         3180 c         3401 g         4918 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagtg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggatctactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctgcattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacaaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccacagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagaat gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa aacatagaaa aacgattact ttgcttatac aacaaggctt ttatggtttt
    16201 gtatcaaaaa aatatttgtt taaatgttac aaatattaaa taattcgtta ttttgtcatt
    16261 aaatgtcatg tttcttttac ctatgaaata tttctaaatg ataaagttaa tagcgtcgaa
    16321 agaataaaaa acggagaaaa aaaggagttt aatatgaatt catttaagaa aattgtttta
    16381 ggtacaactt ttgctgctgg tgctactgca atgtttgtag gaactacaaa tgctcacgct
    16441 gatgaagttt atacagtaaa atcaggtgat tcattatcta agatctcaca aaaatttgca
    16501 ggagacaact caatgatcga tgcaattgct gaaaaaaatt cgatcgcaaa catcaaccgc
    16561 atttatgttg gtgaacaatt aacaatccca acttcaaatg attcttcagc aacaacagaa
    16621 aacaaaacaa cagaaaatac agcatctaca actgaaacag caacgcaaga acatacatac
    16681 gttgcacctg ttgaaacagt tgaagtagct cctgctgccc cagctgctgc aacagcgcca
    16741 acttcaagct ctgcaaaaga atggatcgca caaaaagaat caggcggttc atacacagct
    16801 actaacggac gttacatcgg ccgttatcaa ttagatgctt catacttaaa cggcgactat
    16861 tcagctgcca atcaagaacg tgtagcagaa caatatgtaa catctcgtta tggttcatgg
    16921 gatgctgcta aaacattctg gttagcaaac ggctggtact aagccgataa aaggaaaatg
    16981 atgaaaccga gaaacttgtt tattcaagtt ctcggttttt ttgtgtgaaa aataactcgt
    17041 ttgcttccca cagcgcagca gtacaaataa aaactcgaat aatgt
//
LOCUS       DAFFFQ010000061        17006 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000061
VERSION     DAFFFQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.061"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..125)
                     /locus_tag="LQB69_002077"
     CDS             complement(<1..125)
                     /locus_tag="LQB69_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HBL3526225.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="LQB69_002078"
     CDS             complement(229..540)
                     /locus_tag="LQB69_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3526226.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="LQB69_002079"
     CDS             complement(542..739)
                     /locus_tag="LQB69_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL3526227.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="LQB69_002080"
     CDS             complement(1133..2860)
                     /locus_tag="LQB69_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL3526228.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="LQB69_002081"
     CDS             complement(2853..4046)
                     /locus_tag="LQB69_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL3526229.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="LQB69_002082"
     CDS             complement(4233..4877)
                     /locus_tag="LQB69_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3526230.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="LQB69_002083"
     CDS             complement(4971..5105)
                     /locus_tag="LQB69_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL3526231.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="LQB69_002084"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="LQB69_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL3526232.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFFMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="LQB69_002085"
     CDS             complement(7236..7709)
                     /locus_tag="LQB69_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL3526233.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="LQB69_002086"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="LQB69_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL3526234.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="LQB69_002087"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="LQB69_002087"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL3526235.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="LQB69_002088"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="LQB69_002088"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL3526236.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="LQB69_002089"
     CDS             complement(11756..12895)
                     /locus_tag="LQB69_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3526237.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="LQB69_002090"
     CDS             complement(13204..13917)
                     /locus_tag="LQB69_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL3526238.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="LQB69_002091"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="LQB69_002091"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL3526239.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="LQB69_002092"
     CDS             15106..16638
                     /locus_tag="LQB69_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL3526240.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..>17006)
                     /locus_tag="LQB69_002093"
     CDS             complement(16829..>17006)
                     /locus_tag="LQB69_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526241.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3494 c         2910 g         5323 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga aaaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aataca
//
LOCUS       DAFFFQ010000062        16903 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000062
VERSION     DAFFFQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.062"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..189)
                     /locus_tag="LQB69_002094"
     CDS             complement(<1..189)
                     /locus_tag="LQB69_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526242.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(199..1014)
                     /locus_tag="LQB69_002095"
     CDS             complement(199..1014)
                     /locus_tag="LQB69_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3526243.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1276..1719)
                     /locus_tag="LQB69_002096"
     CDS             complement(1276..1719)
                     /locus_tag="LQB69_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL3526244.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1853..2191)
                     /locus_tag="LQB69_002097"
     CDS             complement(1853..2191)
                     /locus_tag="LQB69_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3526245.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2179..2556)
                     /locus_tag="LQB69_002098"
     CDS             complement(2179..2556)
                     /locus_tag="LQB69_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3526246.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2780..3973
                     /locus_tag="LQB69_002099"
     CDS             2780..3973
                     /locus_tag="LQB69_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526247.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4139..4561)
                     /locus_tag="LQB69_002100"
     CDS             complement(4139..4561)
                     /locus_tag="LQB69_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526248.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="LQB69_002101"
     CDS             4956..5150
                     /locus_tag="LQB69_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526249.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5641
                     /locus_tag="LQB69_002102"
     CDS             5147..5641
                     /locus_tag="LQB69_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526250.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(5803..6975)
                     /locus_tag="LQB69_002103"
     CDS             complement(5803..6975)
                     /locus_tag="LQB69_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL3526251.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="LQB69_002104"
     CDS             complement(7181..7996)
                     /locus_tag="LQB69_002104"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL3526252.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNMI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="LQB69_002105"
     CDS             complement(8146..8592)
                     /locus_tag="LQB69_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL3526253.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9593)
                     /locus_tag="LQB69_002106"
     CDS             complement(8589..9593)
                     /locus_tag="LQB69_002106"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3526254.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9633..9962)
                     /locus_tag="LQB69_002107"
     CDS             complement(9633..9962)
                     /locus_tag="LQB69_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL3526255.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10088..12415)
                     /locus_tag="LQB69_002108"
     CDS             complement(10088..12415)
                     /locus_tag="LQB69_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3526256.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(13258..13551)
                     /locus_tag="LQB69_002109"
     CDS             complement(13258..13551)
                     /locus_tag="LQB69_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL3526257.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13954..15132)
                     /locus_tag="LQB69_002110"
     CDS             complement(13954..15132)
                     /locus_tag="LQB69_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3526258.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(15236..15550)
                     /locus_tag="LQB69_002111"
     CDS             complement(15236..15550)
                     /locus_tag="LQB69_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526259.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            15657..15872
                     /locus_tag="LQB69_002112"
     CDS             15657..15872
                     /locus_tag="LQB69_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526260.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(16172..16414)
                     /locus_tag="LQB69_002113"
     CDS             complement(16172..16414)
                     /locus_tag="LQB69_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526261.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(16401..16829)
                     /locus_tag="LQB69_002114"
     CDS             complement(16401..16829)
                     /locus_tag="LQB69_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL3526262.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFLI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         4926 a         3271 c         2937 g         5769 t
ORIGIN      
        1 gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc ttccatctat
       61 cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac aaaggctact
      121 tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt caaaacgctg
      181 ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt ctacaaaata
      241 tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg aggcagccat
      301 gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta actggattgt
      361 tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc atgtattaac
      421 gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg
      481 atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag aatctgggat
      541 actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt gtaatagcac
      601 caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact gcgggatcat
      661 ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc
      721 tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca actgaatcaa
      781 cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag gaatacgaga
      841 ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa tcggaatcgt
      901 gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt
      961 caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg gcatgttttt
     1021 tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat aatcttcatt
     1081 tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt tccgttcttc
     1141 ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt tttgatttaa
     1201 aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg aaatttcttc
     1261 gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca attcattgac
     1321 tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt caatcattgg
     1381 gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt ctatgactaa
     1441 agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat tgtacgttcc
     1501 tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc gctgtatttc
     1561 tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt
     1621 ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc ttggtaacaa
     1681 aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat ctaacaggct
     1741 tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa attgaacgct
     1801 gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat tatcagcttc
     1861 cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct cgaattcttg
     1921 agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga aaataacctg
     1981 cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct agttcctctt
     2041 ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact tctgcggctt
     2101 gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt aactccatag
     2161 cgatgctcct tgattttttt acttgataca ttccatttta tttctagata acacacgatc
     2221 atatttcttg atttttcctt gtaaacgttc caaacactct tgcatttcag caatttcttt
     2281 cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag ttgcttctcc
     2341 ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg catttcgcat
     2401 acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga ttccgccttt
     2461 tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg tatcggccga
     2521 aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc tcttaatttc
     2581 ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc caagtcaata
     2641 ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta ttatttcaaa
     2701 taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt agggaaattt
     2761 taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag gtattattat cgacatcggc
     2821 gggaattgct ttagagaata tggatattat gtttttagct ttttcattat cttcaatgat
     2881 tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca ctaatttggg
     2941 gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac gtgtgaaagt
     3001 attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa
     3061 catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag gagaatacgg
     3121 ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc
     3181 ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag ctgctgtgat
     3241 cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg tgttgatggt
     3301 cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac aatctgccga
     3361 aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc aaacatgtgg
     3421 ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga attggctgcc
     3481 ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt ggatggtcag
     3541 tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg ataaattggg
     3601 gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct atctacttgt
     3661 gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct actttatcaa
     3721 tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag aaatccgcgc
     3781 gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt cttctatcgt
     3841 catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt tgagcggtat
     3901 ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat taagggaaag
     3961 cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca aaagcttgca
     4021 tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt ttggctggtc
     4081 gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct ggcggcgact
     4141 attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa cgaaaaactg
     4201 tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca ggatgttgat
     4261 acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga taaataatgt
     4321 agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca ttcttcaaaa
     4381 tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa ttcaagatag
     4441 acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa cgttctgttt
     4501 caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa aaagtctgca
     4561 tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc gaagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcatgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    12421 ctctcaataa aaaatcatca aataatcctt tacacgttat tctaaattta atttttatta
    12481 ttttattgat gtatagaaag tttggggctt tttttctctt tttattctaa ggttatttta
    12541 gtcttgtcct ttaatacaaa gattttattt ttaaataaaa aatactcttt ttttacgatt
    12601 attgaaaaaa aaagataaaa cttgattaat tgaagtattg cttaaatttg gaagctatga
    12661 gggaagaata aaagagttag gacgggatta tctactattt ttctttgttt taaatatttg
    12721 ttatctttga acataaaaga gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat
    12781 aaacggtgtt caattagctg atcttgtatt cacttaaaaa atggtaaagt acgagttgct
    12841 attccccatt ttcaattttc taacgctcag gacaaaatac tgttttcaca acctcattgg
    12901 aataaaagca tatatattgt ttcaagaacg aaatcttgtt atctttttaa agggcatctg
    12961 tccaaaatct aattttagga ggtacgtttt ggtatttatt cgtacaaata aaagagaatt
    13021 atctttattc atgtaacgca tccaattgat tttttcatgt acgctcctgg aacgatttta
    13081 ctgacgattg cttatttaac tgcgaatcgc tcattagctt ttatcatagc tatccatatt
    13141 ttgaataacg ttcttggctt tgtattatag atacagatga caaaaaaagt tcaagtatgc
    13201 ggtgtagaac caatatcgca tacttgaact tttttcaatg agtataaatt aaaacttcta
    13261 attttcaatt cccatcatct gatatgcccg accgtctgaa cttcttttga aaaagccaaa
    13321 tgtgattaga taacgtcttt gttcgacata atcgccacta gaaaatacgt tcagcagtat
    13381 cgaattaacc tctgtttctg tataagtcac tccatggtca aagcgtttca agacttcttt
    13441 caataggatg tatttctttt ttgttgaact tggtacttgt tttacttttc catcattcat
    13501 gaatgtgtct aaaactttca tcttttcttg ctccgtataa gtataaacca tcttctttct
    13561 ccttttgatt ctaagaatac tccatttatg ttagaatatt ctggattttt tattaaagcg
    13621 gttactctta agaaagatat tatcatagtg ctcttttttt tactactact tataggtaga
    13681 tttattctaa tccattattc tcccctacca ataattaaag aaaaaactgg atcagggtgt
    13741 tcaaattaca tgctaagaaa aaagaaccct tgaataccaa gggttctttc tcagtctgat
    13801 aacaatgatt aacgacggat ttctttgata cttgtgaaaa gtcgttaaag tattgatata
    13861 aaaggatttt tattacctct gactacatat tgactaattt gttaaaaaat cgtttttcaa
    13921 gaatcctgta ataccagtgc tttcaccgtt tttttaactc tctaaagaac taaaagcaat
    13981 cagaggtttt accttttcat ttgacgttaa gtggcgataa attttcgaag ttgtttggtc
    14041 agtatggcct aagcgatctt tcaagacttc ccagctacag tgttccgcta ctaagcttgc
    14101 cattgtatga cgagctaaat gagtatgaaa tggcttatta attcctgagc gattcacgca
    14161 ccgtttgacg tgatcattta catactctcg acgataagga acaccacgtt tactagaatc
    14221 gagatacaca aacaaatatg tttcttctat aataaacctg taatgttgcc tcatctcacc
    14281 caaagcctta atcaactgtt gtagcttttc cataactaga ggagttacta gaactcgtct
    14341 agcagaacta actgtcttag tagtttcaat tcgaaaatcc ttactcttac ctttaactgc
    14401 cgtttgctta tagaacagaa tttcatttct tttgaaatct atatctccag gctgtagagc
    14461 aagcgcctcg ctgactcggc agccagtgta aaatagaaca tggaaaagta cagacatcgg
    14521 taattcctca tgaacaatgc cataattcaa aaaagttctc aattcatcga cagtaagaaa
    14581 cttgtcctca atattattta tggcttcttt taaatcagtt accgttaact gatattttgg
    14641 cattctcaca gacttcattg gattttcact tatcaaattc tttctttgac aatatttaaa
    14701 aatcatattt agaacttgaa taagtgattg taaactattt ttggcatagc caacatttgt
    14761 tccattaacg ccacattcct ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa
    14821 atcagacaac tttttatcac caatccatcg aacaataatt tttaattgtt cctctcgctt
    14881 atcataggtt atttcctttt gttcatttaa cgaacggtaa tattggaacc attgctcacc
    14941 tacaacacta aacttgtcat tgcttgcaac aatttttttc ttaagccttt gttgtttctc
    15001 catttccaga taagcgagtt gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg
    15061 atatgcttga aagtaccatg tgatcctacc agtagaactg atttgcttct tgaatcgatc
    15121 tgttttttcc atttattgat tcctcctatt ttgaaggaac ccaacaagca gaaacccttt
    15181 ggaaatcttt ctatctatgt catcattgta ccgcatctta gaaagaaaat gtaacttata
    15241 aagatcgaag tagtcgcaat ttttctcgat agtcattcga atcactcttt ctcatccatt
    15301 ctaaaacatc atcacgaaaa tagtgtcctc gaatcccgta ccaacaggga ggaaactcac
    15361 caagttttac taaacggttc agtgtactat cacttatctg aaatagtcgc tttaaatcag
    15421 tatttgttaa taccagaggt aaatgactga ctgcaagcaa tttttccgtg taatcttcca
    15481 gttttttttg taaaattgaa tagaactcta tttccagaga attaacattt gttttaactt
    15541 ccaactccat cacccttctc tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt
    15601 gtaaaaaaac actccagtac tccatcaaaa cggactagaa aagaggaatt tttaatatga
    15661 aacaaggaat tgcagacata aaaataatta aagaaattct tgaaaaatct acggctaatg
    15721 caatagcatt cggaacagga atcaacttga gtacagtaaa aaaacttaaa agtggagaga
    15781 gagctgaaga aaaattaaat cttgccgatg ctattaaaat cacagaattt ggcatgaaaa
    15841 atatgccaac caaaatcgaa atatggaaat agtagttttt ctgatctatc agctgttttg
    15901 ccaaagcaca taggaatctc acctcccccc agtaatggcg ggccgatttg gttacggaag
    15961 tatcattatc gtagtcgcga ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc
    16021 atggcgtaac tagagaatca aaatctacat gtactgatag atttgctatt catttgtcaa
    16081 agagcgctag gctattttca gcctatggaa atcctttatt ttgacccgat aaaaaacttc
    16141 catagaatga aaaggaaagg gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct
    16201 tctcaatcaa ttttgtttcc agttgtctac gcatatattc atctacatac ataaccattc
    16261 ctttttcttc ttcataaact ggtttcatgg ctaaccgtga aatataattt tgatattttg
    16321 acagaacgat ggacatggca attgaatcac cagaagctgc ggctttgatt gtcgttagtg
    16381 gtatgaatgg tatatatcgt ttattttgct tcatatttga cacccaaata ctttcctagc
    16441 tcttccaaag ccatttgtcg ttggtaccaa acactgccga gacttttccc aataatatcg
    16501 ccaatttcac gatcattaag accgatgagg aaggacataa aaatgatttc ttgtctttga
    16561 ctaggtaatg ctgcaatcgc atttgccaac tcctcattgg aaataaagat tttcatacca
    16621 aggacaataa aagtaccact actattcaaa tttggttcca aataatagaa ctctgtacct
    16681 aacccttttt gttccaatat ttccattgat atttgccgtt ggcgaaatct agcgtacttc
    16741 ttttgaatat ttaacgcctc attgcagact actttcttac aaaaactgtc aaacgtatgt
    16801 tgaatgtggg tttcctctga gtcgcccata atattctccc cctcaaaaaa ttcattcaac
    16861 tcgttttact tctatccctt tccattatta ctacaatcta gga
//
LOCUS       DAFFFQ010000063        16731 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000063
VERSION     DAFFFQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.063"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1412
                     /locus_tag="LQB69_002115"
     CDS             <1..1412
                     /locus_tag="LQB69_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL3526263.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="LQB69_002116"
     CDS             1546..2889
                     /locus_tag="LQB69_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3526264.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="LQB69_002117"
     CDS             3001..4452
                     /locus_tag="LQB69_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL3526265.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="LQB69_002118"
     CDS             4801..5433
                     /locus_tag="LQB69_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL3526266.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="LQB69_002119"
     CDS             6021..7460
                     /locus_tag="LQB69_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526267.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="LQB69_002120"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="LQB69_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8222..8437
                     /locus_tag="LQB69_002121"
     CDS             8222..8437
                     /locus_tag="LQB69_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526268.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="LQB69_002122"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="LQB69_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9097..10008)
                     /locus_tag="LQB69_002123"
     CDS             complement(9097..10008)
                     /locus_tag="LQB69_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3526269.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="LQB69_002124"
     CDS             complement(10027..11031)
                     /locus_tag="LQB69_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3526270.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="LQB69_002125"
     CDS             complement(11028..13151)
                     /locus_tag="LQB69_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3526271.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..15603)
                     /locus_tag="LQB69_002126"
     CDS             complement(13156..15603)
                     /locus_tag="LQB69_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3526272.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15590..15979)
                     /locus_tag="LQB69_002127"
     CDS             complement(15590..15979)
                     /locus_tag="LQB69_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3526273.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16039..16542)
                     /locus_tag="LQB69_002128"
     CDS             complement(16039..16542)
                     /locus_tag="LQB69_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3526274.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16555..16716)
                     /locus_tag="LQB69_002129"
     CDS             complement(16555..16716)
                     /locus_tag="LQB69_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526275.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         5411 a         3117 c         3086 g         5117 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    14101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    14161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    14221 ggtaactgaa cccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    14281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    14341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    14401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    14461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    14521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    14581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    14641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    14701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    14761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    14821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    14881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    14941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    15001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    15061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    15121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    15181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    15241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    15301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    15361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    15421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    15481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    15541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    15601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    15661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    15721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    15781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    15841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    15901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    15961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    16021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    16081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    16141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    16201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    16261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    16321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    16381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    16441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    16501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    16561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    16621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    16681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAFFFQ010000064        16700 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000064
VERSION     DAFFFQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.064"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            176..403
                     /locus_tag="LQB69_002130"
     CDS             176..403
                     /locus_tag="LQB69_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL3526276.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            419..1585
                     /locus_tag="LQB69_002131"
     CDS             419..1585
                     /locus_tag="LQB69_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBL3526277.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1820..2863
                     /gene="hrcA"
                     /locus_tag="LQB69_002132"
     CDS             1820..2863
                     /gene="hrcA"
                     /locus_tag="LQB69_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL3526278.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2957..3520
                     /gene="grpE"
                     /locus_tag="LQB69_002133"
     CDS             2957..3520
                     /gene="grpE"
                     /locus_tag="LQB69_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL3526279.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDRYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3571..5400
                     /gene="dnaK"
                     /locus_tag="LQB69_002134"
     CDS             3571..5400
                     /gene="dnaK"
                     /locus_tag="LQB69_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL3526280.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGKRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5551..6717
                     /gene="dnaJ"
                     /locus_tag="LQB69_002135"
     CDS             5551..6717
                     /gene="dnaJ"
                     /locus_tag="LQB69_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL3526281.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(7085..7822)
                     /locus_tag="LQB69_002136"
     CDS             complement(7085..7822)
                     /locus_tag="LQB69_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL3526282.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(7824..9326)
                     /locus_tag="LQB69_002137"
     CDS             complement(7824..9326)
                     /locus_tag="LQB69_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL3526283.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIEKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            9585..11579
                     /gene="uvrB"
                     /locus_tag="LQB69_002138"
     CDS             9585..11579
                     /gene="uvrB"
                     /locus_tag="LQB69_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL3526284.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            11589..14405
                     /gene="uvrA"
                     /locus_tag="LQB69_002139"
     CDS             11589..14405
                     /gene="uvrA"
                     /locus_tag="LQB69_002139"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL3526285.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            14668..15933
                     /locus_tag="LQB69_002140"
     CDS             14668..15933
                     /locus_tag="LQB69_002140"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL3526286.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYAGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            15937..16668
                     /locus_tag="LQB69_002141"
     CDS             15937..16668
                     /locus_tag="LQB69_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL3526287.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
BASE COUNT         5504 a         2901 c         3661 g         4634 t
ORIGIN      
        1 tttttatgaa taaaataagg acaaaattgt ttaggaagaa tcatgagtgt aaaaaaatca
       61 tccaagaagt cgattcttgg attttttagt ggatttgttc gccaaatacg tttagtatgc
      121 gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca cttaaggagg aatctgtgaa
      181 aatagctttt catatttttg cagattttga cttaatgccg aaggtcagct ttgcgattac
      241 cgtttatcga gaggctgtaa aagcaccgtg tagctccgat gacaataagc aagcattttt
      301 agagctaggt ttccttgatt ctaaatatgc ggcttatttc aatgagttgg tgcttgaacg
      361 cgattttaag aaggtcgaaa acaggcacga cttttcgacg taaggagaaa gaatttcaat
      421 gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg tacattcata tcccattttg
      481 cgagcatatc tgctattact gtgactttaa taaagttttt ttagaagggc agccagtaga
      541 tgagtatgtg gaaatgctgc tgaatgaaat gagattaatg ctggaaagtc atcctacagc
      601 gaatttggaa acactttatg taggaggcgg aacaccaact tctttatctg ctaaacaact
      661 tgatcgacta ttatcaggtg ctagagagat cctgcctttt caaaatggta atgagtttac
      721 tgtagaagcg aatcctggtg atttgacccg tgaaaaactt caagtgatga aaaactatgg
      781 cgtcaatcgt ttatccatgg gagtgcagac atttgacaat cgcttattga aaaaaattgg
      841 acgtaaacat actgcagaag atgtgtatca aacaatgcaa tttttagaag cagaaaattt
      901 taccaatgta agcatcgatt tgatttatgc attaccggga caaacactag aaggatatcg
      961 ggatactttg gaaaaagctt tggctttgga tctgcctcac tattctttgt actcattgat
     1021 cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa ggaagactcg aactgcctga
     1081 tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa gcaatggaaa agcatggacg
     1141 tcaccaatat gaaataagta actttgggct ctccggacat gaatctcaac ataacctcat
     1201 gtactggaac aatgaccact attttgggtt tggagctggt gctagcggtt atcttggaga
     1261 tattcgttac cgcaacaaag gaccgatcca acactattta cgcccattac gagcaaatga
     1321 acgcccggta ttggaagaag aaaaattgac acgaaaaaat caaatagaag aagaaatgtt
     1381 cttaggacta agaaaaaaaa cagggattac taaaaaacat ttctatgatc gctatcaaaa
     1441 aacaattgaa tcggtgtacg gtgatgtgct aagggaatta gagcaagaag ggttacttgt
     1501 taatgaagaa gaccgtgttt atttgacacc aagagggact tttttgggga acaatgtgtt
     1561 tgagcgcttt ttatttgaaa aatagtttga agaatatttg ttttttatcg aaacttgaaa
     1621 agaaaaagtt tagaaagttt gacaatgagg tattgaagta ttgatataac tggatttaca
     1681 tcaattattt agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt
     1741 gacaggagaa gggcttcttg ctatatttat aagtgaatta gcacttagaa tatatgagtg
     1801 ctaatgagag gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat
     1861 ccaacactat acgagtagtg gcgtgcccgt tggttccaaa acattaatgt ctgagggagt
     1921 caaggccagc tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct
     1981 gttgaagact cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt
     2041 ggatcatttg ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc
     2101 tctaggcaga gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc
     2161 tgagctgaca agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac
     2221 tggctttcgt atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat
     2281 ggggaatgta gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga
     2341 aaaaatggta cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca
     2401 taaacttcgt acggagattc cgatgattct tcataaatat ttccagacga cagaagggat
     2461 gtctcagtta tttgacaccg ttcttggtca tgtttttgaa gaaaaggttt atgtcagtgg
     2521 acaaatgaat ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt
     2581 ttcttttatg aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat
     2641 tcatatcaag attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca
     2701 agcaagttac gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag
     2761 tatgccttat tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa
     2821 acttgctgat tattaccgct cgcttgactt gtcaaactcg taatgtgaat aacgaagcta
     2881 tttgcagctg atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg
     2941 aaaggaagac tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc
     3001 tcagccagaa ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga
     3061 agcggaagaa ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagatagata
     3121 cttgcgggct cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca
     3181 attacaaaaa taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct
     3241 ggaacgtgca ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt
     3301 ggaaatggtc ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc
     3361 tgctaaagga gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac
     3421 tgaagataca ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga
     3481 tcgcgtgtta agaccaacta tggtcattgt tgcacaataa ttgtgcggat ataaaactta
     3541 cactttcaat taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt
     3601 acgactaact ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca
     3661 gaaggaaaac gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg
     3721 gaagttgcaa aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat
     3781 atcggtgaag caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc
     3841 tcagcaatga tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc
     3901 gaaaaagcag ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa
     3961 gatgctggta agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct
     4021 gctcttgcct atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt
     4081 ggtggcggta cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg
     4141 tcaacagctg gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac
     4201 atggtagcag aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta
     4261 caacgcttga aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca
     4321 caaatcagct tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca
     4381 ttgacacgtg caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca
     4441 gtacgtcaag cgttaaaaga tgcaggtctg tcacaatcag aaatcgacga agttatttta
     4501 gtcggtggat ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa
     4561 gaaccaaaca aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt
     4621 ggcgtgatca ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta
     4681 ggtatcgaaa caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca
     4741 acaagtaaat cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat
     4801 gtattacaag gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg
     4861 acagatattc cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat
     4921 aaaaacggga ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc
     4981 acgatcaaat cttcttcagg tctaacagat gaagaaatcg aacgtatggt gaaagatgca
     5041 gaagcaaacg ctgaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta
     5101 gacgcattgc tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa
     5161 gaagaagtga aaaaagcaga aacagctcgt gacgaattaa aagccgctgt tgaagcaaac
     5221 aacatcgagg aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact
     5281 gttaaactat acgaacaagc tgcacaacaa caagctcaag aaaatccaga agcagctcaa
     5341 ggcggagcag atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa
     5401 tcagttcact gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa
     5461 atagtttgaa gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga
     5521 aaatgatgag aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc
     5581 ttagggctct caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg
     5641 aaaaaatacc atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta
     5701 tcagaagcat atgagatttt aagtgacccg caaaaacgcg ctgcatacga ccaatacggg
     5761 catgcaggca cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc
     5821 ggcggcttct ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga
     5881 ggaggcggtc gatcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg
     5941 gtcgacttgt cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg
     6001 gaagaaatct gtcatacttg tggtggaaac ggtgcaaaac caggcacaca accaactact
     6061 tgtcataaat gtcatggtgc aggtacgatc aatgtagaac gccaaacgcc acttggacga
     6121 gtgatgagcc gtcaaacttg tgatgtttgt catggcacag ggaaagaaat caaagaacct
     6181 tgtccaacct gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta
     6241 ccagctggtg tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa
     6301 aacggcggac catttggtga tttgtatgtg gtctttcgtg tagaagaaag tgatatcttt
     6361 gatcgtgaag gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg
     6421 ggggatgaag tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga
     6481 acacagacag gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt
     6541 gcaacaggcg accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa
     6601 caaagagaag cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa
     6661 gaagacggtt tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt
     6721 ttctagtaaa aatataaagt agacttgccc aaattttcta tttctagtaa aaacgctaag
     6781 tcattaataa ttaaaaacca gacgaacaat catacgttcg cctggttttt ttgtagttgt
     6841 catgtttgta ccaacaaaaa agccagcttt agaataagat aatcatcata gtttctgaag
     6901 gtggtatcga ctatgtactt gatatcgtca atgaaccgat ggtactttct tatgcgatgg
     6961 aacaggtaga gcgattgctt gtttgtgcag gagaacaagc gatgcggctg gtacagttaa
     7021 gtgaaaaaac aagaaactaa aaatgcccgg tcgtcatgaa gcaaccgggc atttttggtt
     7081 tttattaaat attcaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
     7141 caaagatttg ttgaggcgtt ccgtcttcta aaaagtttcc gtcgtcaatg aacatgacac
     7201 gattggccac ttctttagcg aagcccattt cgtgcgtaac gataaccatc gtcatacctt
     7261 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcgggg tcgagtgcag
     7321 atgtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
     7381 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
     7441 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
     7501 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
     7561 gctggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
     7621 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
     7681 gcaaaaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
     7741 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
     7801 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
     7861 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
     7921 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
     7981 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
     8041 atgcgtattg cttgtggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
     8101 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
     8161 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
     8221 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
     8281 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
     8341 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
     8401 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
     8461 agaaatgttg attcatcagc agtggaagac tcactagaag tttctgaacc atccgctaca
     8521 tattctttga taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
     8581 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
     8641 gattcttttt cgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
     8701 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
     8761 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
     8821 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
     8881 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
     8941 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
     9001 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
     9061 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
     9121 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
     9181 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
     9241 tcagcttgtg caacatgacc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat
     9301 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
     9361 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
     9421 gtttctaaca ttttatgtta taaaacgtaa catgtataat aagataaaaa agtggcgaaa
     9481 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
     9541 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
     9601 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
     9661 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
     9721 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
     9781 tgattattgc tcataataaa acattggctg gtcagctgta cggggaattc aaggaatttt
     9841 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
     9901 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
     9961 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    10021 ccgtttcatg tatttttgga ttaggttcgc cttttgagta ccaaaaacaa gtagtttcga
    10081 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    10141 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    10201 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    10261 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    10321 tggcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    10381 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    10441 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    10501 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    10561 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    10621 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    10681 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    10741 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    10801 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    10861 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    10921 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    10981 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    11041 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    11101 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    11161 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    11221 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    11281 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    11341 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    11401 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    11461 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    11521 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    11581 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    11641 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    11701 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    11761 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    11821 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    11881 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    11941 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    12001 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    12061 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    12121 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    12181 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    12241 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    12301 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    12361 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    12421 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    12481 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    12541 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    12601 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    12661 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    12721 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    12781 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    12841 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    12901 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    12961 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    13021 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    13081 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    13141 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    13201 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    13261 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    13321 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    13381 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    13441 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    13501 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    13561 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    13621 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    13681 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    13741 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    13801 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    13861 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    13921 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    13981 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    14041 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    14101 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    14161 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    14221 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    14281 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    14341 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    14401 tataaagagg atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg
    14461 ttttattaat atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta
    14521 tagataaaaa cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact
    14581 ttcctttgat tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa
    14641 caagagatta tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc
    14701 cctatattat taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca
    14761 tacggcaaaa tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa
    14821 attgtcaatg tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg
    14881 cttcgtttgg caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt
    14941 ggtgatggat atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc
    15001 tgtccatata ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa
    15061 gtttgtgaag aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta
    15121 gtaaatggac atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca
    15181 aacagtgtgg agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag
    15241 actagagaag agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa
    15301 atgattgtac aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat
    15361 gtagatgaag atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct
    15421 acaccagcat cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat
    15481 caaaccatta aggcattttt ggaaaaaatc gaatatgcag gctttgccaa tttcgatatg
    15541 aagtatgatc ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt
    15601 agcagtttct ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga
    15661 gtgttcaaca aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga
    15721 ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa
    15781 aaactagctg agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa
    15841 gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga
    15901 ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta
    15961 ggcggaatgg gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa
    16021 gctacaaaag atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat
    16081 cgtacagctt atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat
    16141 attgagaaac aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac
    16201 tacttttttg aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa
    16261 gcagcaaatg aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa
    16321 ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg
    16381 attcctgata ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa
    16441 tcggattatt tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta
    16501 aactgtgaaa aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa
    16561 gataatcatt acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt
    16621 gcattagctg aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt
    16681 tggggcaaat atcctaaaag
//
LOCUS       DAFFFQ010000065        16586 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000065
VERSION     DAFFFQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.065"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..495
                     /gene="scm"
                     /locus_tag="LQB69_002142"
     CDS             <1..495
                     /gene="scm"
                     /locus_tag="LQB69_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL3526288.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSK
                     TSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYK
                     KSKR"
     gene            complement(650..1636)
                     /locus_tag="LQB69_002143"
     CDS             complement(650..1636)
                     /locus_tag="LQB69_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL3526289.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1659..2225)
                     /locus_tag="LQB69_002144"
     CDS             complement(1659..2225)
                     /locus_tag="LQB69_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL3526290.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2239..2655)
                     /locus_tag="LQB69_002145"
     CDS             complement(2239..2655)
                     /locus_tag="LQB69_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL3526291.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3112..5046)
                     /locus_tag="LQB69_002146"
     CDS             complement(3112..5046)
                     /locus_tag="LQB69_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3526292.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5327..6283
                     /locus_tag="LQB69_002147"
     CDS             5327..6283
                     /locus_tag="LQB69_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL3526293.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6373..7476
                     /locus_tag="LQB69_002148"
     CDS             6373..7476
                     /locus_tag="LQB69_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL3526294.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7489..8442
                     /gene="menA"
                     /locus_tag="LQB69_002149"
     CDS             7489..8442
                     /gene="menA"
                     /locus_tag="LQB69_002149"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL3526295.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9377..10723)
                     /gene="gor"
                     /locus_tag="LQB69_002150"
     CDS             complement(9377..10723)
                     /gene="gor"
                     /locus_tag="LQB69_002150"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL3526296.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10857..12110)
                     /locus_tag="LQB69_002151"
     CDS             complement(10857..12110)
                     /locus_tag="LQB69_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL3526297.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12116..13039)
                     /locus_tag="LQB69_002152"
     CDS             complement(12116..13039)
                     /locus_tag="LQB69_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL3526298.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13260..15752)
                     /locus_tag="LQB69_002153"
     CDS             complement(13260..15752)
                     /locus_tag="LQB69_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL3526299.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15759..16226)
                     /locus_tag="LQB69_002154"
     CDS             complement(15759..16226)
                     /locus_tag="LQB69_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL3526300.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16363..16436)
                     /locus_tag="LQB69_002155"
     tRNA            complement(16363..16436)
                     /locus_tag="LQB69_002155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16400..16402),aa:Arg,seq:acg)
     gene            complement(16494..16567)
                     /locus_tag="LQB69_002156"
     tRNA            complement(16494..16567)
                     /locus_tag="LQB69_002156"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16531..16533),aa:Pro,seq:tgg)
BASE COUNT         4898 a         3467 c         2954 g         5267 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagcacgga cacgtcagct
      181 tcaagtgaca gtacagatac atcctctacc acaagtgata gctcagattc atcaacttca
      241 agcgatagta cagacacgtc gtctaccaca agcgatacac gtcatgcaag tgtctccttc
      301 tcaaaaacaa gttcggataa caggacgaac tttaacttaa ctaataaaac aagcaatgac
      361 aatggaaaca aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa taattggata
      421 acactcgcag gcgttatatt acttgtaatc ggtttaagaa tcacttttat gagttataag
      481 aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta acggacgttg
      541 ctctttattt ttgcttttta tcataaatac aagaactaca attcaatttg tcaaaaaaca
      601 cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt cagtctttgc
      661 gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga gaggtggcag
      721 gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt
      781 cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct tcttctgtca
      841 ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc aaagagtaga
      901 tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg ccgatttcct
      961 ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc ccgatagaaa
     1021 gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca aaagcgccaa
     1081 cagaacaact taattggaac agttcagctg tcttacctga aatattttcc aaatattctt
     1141 ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca ttcaatactt
     1201 tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa tcagacaata
     1261 gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca ttaccaaact
     1321 gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata tcatcatgga
     1381 tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct ttgtctcgat
     1441 cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac agctttccgc
     1501 cggcatggat cgtttctttg atggcttgtt cgacaggttt gtttttcagg cggacagttt
     1561 cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag ttttcccaca
     1621 ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt aaacattggg
     1681 ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag tgtctgcaca
     1741 tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat ccctaaaact
     1801 gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga agccatcaaa
     1861 agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat actgacacgt
     1921 tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc accactcata
     1981 ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag tcgtagccaa
     2041 agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt gattagattg
     2101 gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat attttccaat
     2161 aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt ttgtaattta
     2221 ctcatggtgt gtgaaggtct aataaatcag actgtcttcg tcacctccat ctgttgaacg
     2281 gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt tcgttgccca
     2341 gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta ctcgatcgcc
     2401 atctatctcg atcaagttat aatctccgtg ttcatctttg taagtatatg tgttgctctc
     2461 ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca gtaccgcttc
     2521 tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta aaaaagaaag
     2581 aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt tttcagcaaa
     2641 ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat tttagcataa
     2701 aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac
     2761 agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg tggaacaccg
     2821 tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag gaatttacga
     2881 aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc tacttctgga
     2941 acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag
     3001 gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt gacggtttgt
     3061 ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta tttatttctt
     3121 gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt gtagccatgg
     3181 aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac
     3241 aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg tgatcaccaa
     3301 tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg ctaatgccaa
     3361 ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat tgtttggcat
     3421 gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag ccattggttc
     3481 ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt ctccggcact
     3541 tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac cactggcaac
     3601 atctgttaca ttgtattccc atgtccaagg gaacacactg tttccagtga accatgatcc
     3661 ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt ttttggaagc
     3721 ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata agacattgcc
     3781 gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga aaaactcatg
     3841 cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt tcaaattcac
     3901 gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc
     3961 taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt taaatttatg
     4021 cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc
     4081 agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa agactaaatc
     4141 accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt cattggcgat
     4201 caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag ttcctttgac
     4261 accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg
     4321 atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt gcttacgcaa
     4381 atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg tcggcattgc
     4441 ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg ctaggcgatt
     4501 tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac cacagacaac
     4561 gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt ttgctactgt
     4621 cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg tgaatccgtg
     4681 tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa ggatcagctg
     4741 atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat cgataccgat
     4801 tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct gcagatcgta
     4861 ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg tcatcatagt
     4921 atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca gtttctttgc
     4981 caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa tattttgctt
     5041 tgccattgat catccgctcc ttatagacta tctatataat gaatatatga taattctttt
     5101 agtttgatac ttattacaca atctccatat attatataga tgttctgttc ttttgtctaa
     5161 ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa
     5221 gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta taggctaaaa
     5281 agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg
     5341 tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat tttcagctct
     5401 agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt ctgaatcatc
     5461 tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag ttgtagctgg
     5521 cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa atggttaccg
     5581 tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat atgataacgt
     5641 caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa
     5701 atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct taaaagcaca
     5761 aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa gcttccaaaa
     5821 ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa tcgaaatcga
     5881 caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact actctaacgg
     5941 ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat caaactttga
     6001 ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg aaaatatgaa
     6061 agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg aattcgtcaa
     6121 agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag gtgctacaca
     6181 cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg aaaaaggcga
     6241 cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata atcatactaa
     6301 taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa
     6361 gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg catctctttt
     6421 atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag ttcagcagct
     6481 accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac ttacgttcgt
     6541 atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga tcgagtaaaa
     6601 gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga agtgaatgag
     6661 caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt caagcgggcc
     6721 tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa
     6781 ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat cgatcaggca
     6841 ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta
     6901 aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat tacggacgaa
     6961 gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt gggcggtaat
     7021 gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt cggcatccaa
     7081 gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa caaaacatta
     7141 gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata ccatcatttg
     7201 tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac gatcataaca
     7261 gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa
     7321 ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa
     7381 gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc ggaggactcc
     7441 cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat gacactaaaa
     7501 atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc ttttgcggta
     7561 ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac ttttttattt
     7621 tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta catggattat
     7681 gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg cagaaatcta
     7741 tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt tgttatcggc
     7801 gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat ctgttgtttt
     7861 attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt aggggaaatt
     7921 ttcagtgggg tgactatggg ccttggaatt tttgccatgg tcgtttatgt caatacattc
     7981 ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttga tttggtagga
     8041 aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt tacgattgcc
     8101 aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa tcatcgctat
     8161 acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct attgatgtat
     8221 gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg gccgatcttg
     8281 gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa gcaaacgtta
     8341 ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa tgccagttat
     8401 gtattggggc tattactaac aatcattatg caagccattt aacagcatga agaggttgtg
     8461 aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc cttatatttt
     8521 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta tcaggaggtt
     8581 gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     8641 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt
     8701 tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     8761 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttatcagagg
     8821 ttgtaaaatc agtgttttgt cctgagtatt agagaaacaa atggaaaaat agttcctcat
     8881 attttgccgt tttttgagct aataccgaag gacagctgat tgaacactat ttgttcggaa
     8941 aaagtgacta actatgacac aatttttttc acagtccctt ttttcacttg atttgattcg
     9001 taccagcgaa cttgacgaga actttcttgg tttggtaact atatggtatt cttttaacat
     9061 cctcgtttgc ttcttctaat ttatttatac aaagtcacac ttttttaaac atctgttctt
     9121 cgatacattt ctatttcttt taaacatcga taccaatatt ttgaacttaa gagtgcatac
     9181 caaatggatt catttactct tcgtcagtta cgaagataat caaaatctcc aacttcccta
     9241 cttatttcgt tttgagaatt atcgaagcaa tcttacataa ataactaaaa aacaaaaaac
     9301 cagaaaatca gtatactgat tttctggttt tacatggttt gttcaataaa aagctgttgg
     9361 aactgttaat attaaattat cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg
     9421 ctactgtatt gtcaaagtct tctttagttg cacccatctt gatagccacg gcaaaacctt
     9481 gcagcatctc atcgacacca acaccaatag catgtaagcc gacgattctt tcttcttttc
     9541 cgacacagat caatttcatt tcgcattttt ggcgataatc atttaatgca aagtacattg
     9601 gggtaaagcg tgaacgataa actttcaatt cattttcgcc atatttttcc aatgcttctt
     9661 cttctgtcaa accaattgtt gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt
     9721 tatagtcaag ataagaatct ttctttccat taaataaacg ttctgataaa cgtctaccag
     9781 ctgcgattgc aacaggagtc agatctaatt taccgatcac atcaccaaca gcaaaaatat
     9841 gttcatccgt tgtattttgg tatttatcta ctttgataaa cccttttttg tccaattcca
     9901 catttgtatt ttccagacct aatttttcgg tattcggtac acgtccaccg gcaaaaagta
     9961 cacaatcacc agttaaggtt tcaccatttt caaatagcac cgtatattcg tcattgtttt
    10021 tagtaatttc tttagcaata tgatgtgcgt aagtttttat tccagcttct tggtacattt
    10081 ggatcaaatt gtccgaaagc attttatcaa acgttctaag tggtcgttct tttcgatatg
    10141 cccacatgac ttcactacct aaaccacgaa atacacctga aagttcagcc gcaatgtatc
    10201 cagcacctaa aacgattgct ctcttaggta actctgttaa ttcaaaaaat ccatttgaat
    10261 caattgcgta ttctccacca ggaatggtca ttttacttgc acggcctcca gtagcgatca
    10321 agattttagg agcacgatag gtttcaccgt ttacttcgat cgtttgacta tcgataaaag
    10381 tagcgtaacc ttttatagcc tcgacatgat tgctatctaa tcctctttga taagcaccgt
    10441 gtagaaaatc aatatatttt tctcgtcttt ctactagacc ggcgaaatca acatttttta
    10501 aatctgcttg gataccgtaa cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg
    10561 cttgccacat aactttttta ggtacacaac cgacgttgac acatgttccg cccaattcat
    10621 tcgcttcaat cagcaaaact tttgcgccat gcatccctgc tctgtttgct gaagcaatac
    10681 cgccagagcc gccaccaatt acgatgtaat catatatttg cattttgttc ctccttattg
    10741 aagagatcaa tcaaaacctt tatttaacga tcctttttct acagaaaaca agtgtaacac
    10801 ttttcttgaa ttttgtatgc gaagatgctt aaaacaggga tatatcagct ttttccttat
    10861 ttacgttttc ggatcatatc tccaggtttc accggctgtg cgactggcat agttaaaatc
    10921 atcattggat ttggtgctcg gtcaatggat tcgccttctt cattatgcat aacttcaacg
    10981 gtttgactga aatgactgaa tcctggacca taaaattcga tttcatctcc tacagaaaac
    11041 aagtttctct gacgtatcgt agcaatctgt gttgcctcat cataagataa tacttcaccg
    11101 acaaatttat atactgggat tttacgacgt tcaccgaata attgttcgtt ttcagttggt
    11161 gtattgtagt aaaaacctgt agctaattct cgttgtgcta ctttccataa ctcgtcgatc
    11221 cattcttgct tgcagacata gttttcagga tcttccatat aactgtctac agccgcttta
    11281 tagacatttg acaccgtaga tacataatgg atcgatttca ttcttccttc gatcttgaag
    11341 ctgtctacgc cgttttcaat caattctgga atatgttcga tcattgacat atccactgca
    11401 ctcatcgaga attcttcttc cacttcgcct gtatcagtta aactgtttcg ttcttgacca
    11461 aaaggcatat cgaacaatcc atacttccag cgacaagatt gagaacatcc gccacggttt
    11521 gcatcccgca tcgacatatg gttggacaat gtgcatcgac cagaataaga gatacacatg
    11581 gcaccatgga taaatgcttc gatttcggta tccgtatttt tacgaatctc tgccacttcc
    11641 tccattgaaa cttcacgagc taaaacgaca cgttccaacc cttcttcttt ccagaattcc
    11701 aatgtctcaa aattagttgc tgaagcttga gtggataaat ggatcggcag acctggggct
    11761 tctgtcgcac aaatttcgat caaagctgga tcagaaacaa tcactgcaga aataccgaca
    11821 tcacgcaaac tgcggaaaaa ttcccctgct ccatcttcat ttccttcatg ggtgaccata
    11881 ttagcagcaa cgtatacctt cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt
    11941 tcttcataag taaaattacc tgcacggctt cttaaaccat atgcattccc gccaatatat
    12001 actgcatctg ctccataatg gatcgcggtt tttaactttt ccagtgttcc tgctggcgct
    12061 aggatttctg gtcgtttcaa ttttcttttt actgtcattt ctttcatcat actccttatt
    12121 tgatagcaga aggatcaata taaaagaatc ctgtatctaa ttcacggttt tcaggatgca
    12181 atttactgat ttcgctagtc attttttccg ctttttcttg atcccagcta ccttctaaaa
    12241 tttgtttttt tgcttcggcg aatagtcgag tgatgtttac gaatttttcg cctggagtaa
    12301 ataatccgtc caatttccat gtacagtaat gatgctgata aagttcattc aattcattcg
    12361 agagattcag atcattgctg gcaaaaatat gtgtgccatg actatcttca taaatagaat
    12421 aatgtgtctc ttctttttta ggttccgaga taaaaagacc tctttcacga tctttttgtt
    12481 cgtcttgttt cgtatagtta tagtaattct gtagcagtgg acgtttggat tgatggatgc
    12541 atgtagcacc atacacaagg gtttctacag gaatatcgag tttttcttcc atcgccttca
    12601 tctcttcaaa aggaacttca cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga
    12661 aattgatttg acgtgcgctc gttactaatg tttcagcatc ataaatatac ggtatacgca
    12721 attcaggatt ttttttcatc gtataaataa tacctgtatc accaactgtg atcacatcta
    12781 cgtttatttc ttttaaaaaa gccaaatatt caggtacatt tttcattttt tcaggatgaa
    12841 tgatcccatt tacagcaata ttcgcttttt taccatactt gtgtgctaat tctaccaatt
    12901 gtcgctgttc ttctcgtgta aatgaagcag gcagacgcaa tccaaattgc tcttcaccga
    12961 aatatagtgt atctgcacct gcagcaagca actgttctgc ttgttcaata gtttctactg
    13021 tggttatgag ttcgatcata attttctcct cctacaactt cataatagta gcatagctct
    13081 aaaaactttc caatacttct tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa
    13141 atcaaaataa agttcgatat attctgtcca aatataaaaa gagattgtga caaaaatctt
    13201 gtcacaacct cttaatcaag tcttactatt ataaaaattc agctacacag gccaagtaac
    13261 tatagggaac ctactgtttc ctttttaggt ccgcgaacat tcaatgtgat cttacctttt
    13321 gaagcaccta ctgttacgtg gtcacctaaa cgaatttctc cgccaagcaa agcctcgctc
    13381 aatcgatctt caatttcttt ttgaagtgcg cgacggattg gacgtgcacc atattcagga
    13441 tcaaatccag ctttcccgat cacatcgatt gcagatggtg tgattttcac ttgaatatct
    13501 tgttctttca atcgtttgat aatcgctttg ctcatgattt ggacgattgt atggatttca
    13561 tctttactca atgaatggaa gacgacagtc tcatcgattc gattcaagaa ttcaggtcgg
    13621 aaagctttct ttaactcttc taagatacgt ttttgcatgg ctttgtgatc tttagtaaca
    13681 tcttggacat tgaatccgac atttttttct tctcgaattt gtgtcgctcc gatatttgaa
    13741 gtcataatca tgattgtatt tctaaagtcc acttttctac ctttagaatc tgttaaatga
    13801 ccatcatcca atacttgcag aagtaaattg aaaacgtctg gatgtgcttt ctccacttca
    13861 tctaagagaa tcactgaata tggtttttga cgaatttttt ctgtcaactg tccaccttca
    13921 tcatacccta cgtatcctgg aggagacccg attaatcggc ttgtactgta tttttccata
    13981 aattctgaca tatcaacacg gatcagggca tcttcacttc caaacatgac ttctgaaagt
    14041 gctttggcca attctgtttt tccgacacca gttggtccaa ggaacatgaa tgaaccaatt
    14101 ggacgatcag gatctttcaa tccgcttcgt gcacggcgga tcgcacgtga aactgctttg
    14161 acagcctcat cttgaccaac gacacgttca tgcagaagtc cttccagttc taacaagcgt
    14221 tcgctttctt ttttctccaa ctgctgtaga gggacaccag tccactccga tacaactgta
    14281 gcaacatctt cttctgttac acgatcagaa taaccttgct cctgttttgc ttcaattgca
    14341 gaaacttttt gcagcttttt acgtagtgct ttttcttgtc tgcgtaattg agcagcattt
    14401 tcaaagtctt gtttttggat cgctgcttcc ttttcttgta ctagttcatc gatttctagt
    14461 ttgatcacag ttgattttgt taagtgatcg gtttgatcca aacggacttt cgctgctgat
    14521 tcatcgatca aatcaattgc tttatcgggt agttgacgag aagtgatata acgaacagaa
    14581 agattgactg ctgctcgtac agcttcatct gtgatctcca ctccatggtg ctcttcatat
    14641 cttgtacgta atccttgaag aatcacttct gcttcttcag gagttggctc atctacttga
    14701 atgcgagcaa aacgtcgctc taatgcagaa tctttttcga tatatttttg atattcatct
    14761 agggtcgttg ctccgatcgt ttgtaattct cctcgagcta acgccggttt caagatattc
    14821 gaagcatcaa ttgctccttc tgctccacct gcaccaatta aggtatgcaa ttcatcaatg
    14881 aataagatta cttgaccatc attatagatt tcgtcgatga cctttttcat tcgatcttca
    14941 aattcaccgc gatatttcgt accagcaacc aaagatccca tatcaagcat catcaggcgc
    15001 ttttcttgca tgtcttctgg cacttcacct aaaatgattt tttgagctaa cccttcagca
    15061 atggctgttt tgcctacacc aggttcaccc accagtacag ggttattctt tgtccgtcgg
    15121 ctaagaatct ggataagccg tttcacttct ctcgaacgac caacaactgg gtccagcttg
    15181 ttttcttttg ctaatttcgt caaatcacgt gctaatgaat ctagcgtagg tgttccttgc
    15241 ggtgtttgcc gtttatcttg tacatttaca tttctacgac gattcattcc ttttccagca
    15301 gaatcaccag ctagccccat tttttgtttc agaacttgac gggcttttga aaggctgata
    15361 cctaagttga tcatgattcg agtagccatg atactttcat cttttaataa gctcatcaaa
    15421 atatgttctg ttccaacttt ttgagaacca gttttcttca cttcgctgct agctaaagca
    15481 aaaacttgtt tcattcttgg tgaatatgga agaaagactc ctttagggta agaacggata
    15541 ccaccataac caatcagatg ttcaatttct tcataaattt ccgtttcatt caaatccatt
    15601 tcacgtaata cttttccagc aattccattt tgctcgacca ctaaggctag taaaacatgt
    15661 tctgaactaa ctgattgatg tttcaaccgt tttgcttctt cttgtgcaat ttctaatact
    15721 gcttgggctc tttcagtaaa aagttcttcc atctaatctc acttcccatc ttcataactt
    15781 aaacgttcca gaaatgctcg gagaatccga gcacgtgtat gatcttcata ttcattgaaa
    15841 ttcaatgtgt tttttgcaat tgcactcaac atcaagtttc cttctttttt tgtaattatt
    15901 tggtcttcat aaagtttttg aataatagca aaagcatttt tctcactgat tgtatcatca
    15961 aataactgat ttatctgctc aagtaattgt tttttatctg aaatccttac ttttgcaatt
    16021 cgtatataac caccaccacc acgtttgctt tctactagat aaccacgttg gatggtaaaa
    16081 cgggtattaa tcacatagtt gatttgtgaa ggaacacagt taaagaggtc tgccatttca
    16141 atccttcgaa tctcgatcat ttcactattt tccagaattt tctttatata tgattcaatt
    16201 aaatccgagg tattctgatg tgccatctta ttcattccct ttgactaata ttgaccttaa
    16261 ttataacgaa tttgataata gaaagaaaga agaacgcttt ttgttaaata aatcctttag
    16321 aatcaacaaa aaaaaaatac ggaatatact attccgtatc gacgcgccca agaggagtcg
    16381 aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat gggcgcatat
    16441 tcaatttaaa aaaattataa atcatttgaa aacaaaaagc aagataaaaa aaatcgggaa
    16501 gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca agctgagcta
    16561 cttcccgttt aaaaaataaa aaagac
//
LOCUS       DAFFFQ010000066        16526 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000066
VERSION     DAFFFQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.066"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(141..416)
                     /locus_tag="LQB69_002157"
     CDS             complement(141..416)
                     /locus_tag="LQB69_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL3526301.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /gene="der"
                     /locus_tag="LQB69_002158"
     CDS             complement(659..1969)
                     /gene="der"
                     /locus_tag="LQB69_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL3526302.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LQB69_002159"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="LQB69_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL3526303.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="LQB69_002160"
     CDS             complement(3515..4195)
                     /locus_tag="LQB69_002160"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL3526304.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="LQB69_002161"
     CDS             complement(4244..4879)
                     /locus_tag="LQB69_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3526305.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="LQB69_002162"
     CDS             complement(4945..6348)
                     /locus_tag="LQB69_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL3526306.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="LQB69_002163"
     CDS             complement(6335..7366)
                     /locus_tag="LQB69_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526307.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="LQB69_002164"
     CDS             7397..7618
                     /locus_tag="LQB69_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL3526308.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="LQB69_002165"
     CDS             complement(7783..8520)
                     /locus_tag="LQB69_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL3526309.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="LQB69_002166"
     CDS             complement(8673..10013)
                     /locus_tag="LQB69_002166"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL3526310.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="LQB69_002167"
     CDS             complement(10250..11773)
                     /locus_tag="LQB69_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL3526311.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="LQB69_002168"
     CDS             complement(12151..12762)
                     /locus_tag="LQB69_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL3526312.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="LQB69_002169"
     CDS             complement(13092..13808)
                     /locus_tag="LQB69_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL3526313.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LQB69_002170"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="LQB69_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL3526314.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="LQB69_002171"
     CDS             complement(14367..15164)
                     /locus_tag="LQB69_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL3526315.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="LQB69_002172"
     CDS             complement(15134..15505)
                     /locus_tag="LQB69_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL3526316.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LQB69_002173"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="LQB69_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL3526317.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3576 c         2798 g         5507 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacttgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttcttt
//
LOCUS       DAFFFQ010000067        16332 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000067
VERSION     DAFFFQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.067"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(117..812)
                     /locus_tag="LQB69_002174"
     CDS             complement(117..812)
                     /locus_tag="LQB69_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3526318.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            966..1244
                     /locus_tag="LQB69_002175"
     CDS             966..1244
                     /locus_tag="LQB69_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526319.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1263..2471
                     /locus_tag="LQB69_002176"
     CDS             1263..2471
                     /locus_tag="LQB69_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526320.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2619..2984)
                     /locus_tag="LQB69_002177"
     CDS             complement(2619..2984)
                     /locus_tag="LQB69_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL3526321.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3131..3904)
                     /locus_tag="LQB69_002178"
     CDS             complement(3131..3904)
                     /locus_tag="LQB69_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3526322.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4078..4938)
                     /locus_tag="LQB69_002179"
     CDS             complement(4078..4938)
                     /locus_tag="LQB69_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL3526323.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4938..6356)
                     /locus_tag="LQB69_002180"
     CDS             complement(4938..6356)
                     /locus_tag="LQB69_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526324.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6413..6862)
                     /locus_tag="LQB69_002181"
     CDS             complement(6413..6862)
                     /locus_tag="LQB69_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526325.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7307..7570)
                     /locus_tag="LQB69_002182"
     CDS             complement(7307..7570)
                     /locus_tag="LQB69_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL3526326.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7669..8964)
                     /gene="rho"
                     /locus_tag="LQB69_002183"
     CDS             complement(7669..8964)
                     /gene="rho"
                     /locus_tag="LQB69_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL3526327.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8961..10232)
                     /locus_tag="LQB69_002184"
     CDS             complement(8961..10232)
                     /locus_tag="LQB69_002184"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL3526328.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10397..11266)
                     /locus_tag="LQB69_002185"
     CDS             complement(10397..11266)
                     /locus_tag="LQB69_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL3526329.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11558..13168)
                     /locus_tag="LQB69_002186"
     CDS             complement(11558..13168)
                     /locus_tag="LQB69_002186"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL3526330.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14361..15701)
                     /gene="glnA"
                     /locus_tag="LQB69_002187"
     CDS             complement(14361..15701)
                     /gene="glnA"
                     /locus_tag="LQB69_002187"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL3526331.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15729..16121)
                     /locus_tag="LQB69_002188"
     CDS             complement(15729..16121)
                     /locus_tag="LQB69_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL3526332.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4660 a         3463 c         2776 g         5433 t
ORIGIN      
        1 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
       61 ccgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tgttcatcaa
      121 aagataatat tgtcttctaa caagtccata ttcaaaataa cgatatgatg gtctttaacg
      181 gtgataatac catctttttt cagttgtttt aaaattctgt ttacactgct agcagttgag
      241 atgccacaaa atttggcgat ttcttcattc gtgatcgaaa aatcgatcag catcccttct
      301 tctgtccgca caccgaataa atcatataat tcgtaaagtt gcgtacaaac agctcccagc
      361 tttccgttta atagcatttg ctgcattttt ttcatcgaat aaagcagacg atagcggtaa
      421 tactctttta catagtgttg tagctttggg tcttggttga tattttgcca aaagcgcaca
      481 cgattgattc gataaaactc tgcatattct gattcaactc gaatattata cggcgaatcg
      541 atgaattgtg aatattcgtc ttttaaaaga gagacgattt caagatcttt gatatagcga
      601 aggttaaatt ctctcccatc atttgaaata acacttgctt ttaccgttcc ctctttcaag
      661 atatacgtat gggaatcttc catcccttcg aatgtcaaat atttatgata tcctttgcgt
      721 ataatcggaa aatcattttt atccaaatat tcctttaaga ctctactatc caatgatgct
      781 cccccatcca aactttcatc taaaaagatc atacagtact ttaaaacttt ttaaagtcca
      841 aaggtatttt actaatgata ttaacatttg ttaagtacct ttttccatca cttatgcgat
      901 ttttttcctt ttttttattc gttatactgc agaagtaaaa ttttttaaga atagagggaa
      961 aaaacatgaa ttactggatg aacacaatca ttaatcgatt agaaacagca tatcaaacaa
     1021 gatttgatat gaaagcttct ttagtctttt taaatgatgc gtatcaaaac tcgatcgaat
     1081 tgatcaaagc agtggatgag aatccaacaa acgaatgcga agaattttta aatctgttta
     1141 tgtctacaag agatttattt attcgccaac ttgttgatcg ctatccatcc aattatcacg
     1201 atgtagaagt tcaaatacaa aaattaaaag catattcagc ttaaggggaa tgtataacat
     1261 acatggaaaa gaaattattt aataaatatt ttattggaat cacattgatc aattttattg
     1321 tctacctgat ttattatctg ttgatggtca tcatggcagt cattgcgcaa gacaccttac
     1381 acgccagctt gagccaagca ggttttgctt ctgggatcta tatcatcgga acattgctgg
     1441 cacgcttatt catggggaaa cagttagaac tatttggtcg gaagtttacc ttaagaggcg
     1501 gcgcgatctt ctatctcctt tcaacattag catatcttta tacgccaact atcggggcat
     1561 tgtatttagt tcgattcttg aatggattcg gctacggtac agtttctaca gctacaaatg
     1621 ctatcgttac tgcatatatc cctgcatcta aaaaagggga aggcatcaat tattatggat
     1681 taagtactag tttagctgcc gctgttgggc catttttagg aatgattttg ctgaatatga
     1741 ctgattttcg tttcattatc tggttttcaa ttgtactggt cttctttgtt acgattgcct
     1801 gccttgtctt tcctgtaaaa aatattccat tatctgaaga acaaaaaata tctcttcgct
     1861 catggaatat caatagtttt attgaaaaga aagcactttt cattacagga attgcctttt
     1921 tgatgggtct ttcttattca agtgttttat catttttatc ttcttataca aaagtaatcc
     1981 acttagttgc tgctgggtca tttttcttcg tcgtatatgc tttggtcatc acatttacac
     2041 gtccattgac tggaaggatt ttcgatgtca aaggagaaca atacgtgatg tatcctagct
     2101 atttattctt aacagcagga ttatttttac tgagtgtaac gacgaatagt gtgacattgc
     2161 ttatctctgg agcattagtc ggcctaggtt atggtacgtt tatgtcaaat ggacaggctg
     2221 tttgtttaaa aatcgtccgc gaacatcgaa tcagtattgc tttatctacc tactttattg
     2281 gtttagacct aggcttaggc gttggtcctt atttgatggg gatgctaaaa tctcttactt
     2341 catttagagg attgtatgtg attgccggag tcattccatt gatatgtaca gcactctatc
     2401 ttgttaatgg ccgaaaacat agtcatgaag cagagggcga attgattgaa tcattgaaaa
     2461 atgaaatttg atcttgatga atagataaaa aaaaccgttc agatctgaaa ataatctgag
     2521 cggttttttg agtaaaaata gtttatgatt attcttaatt aaacatctat ttatttttaa
     2581 agctttcatt tatcgataag aatagtttaa ctgttgattt attccatttt ccgttcatct
     2641 ggtacgatgc cttgacttag caaccacttg tggaagaacc attctgccac accgatcaat
     2701 agagcaggaa tcaatacttt catccaaata tcgactgtat cattcataaa atatagatag
     2761 agccacaaga taactgcagc tgaaccagca tctgcgatcg tcgctgcgat gttccctact
     2821 ttacgtaaga tcaacagatc ccctagcaca tagatcatga ctacaataat taccccaaca
     2881 atcgttgaat ccataaagct aacaccgtat aaacctgtca gcacgaccca cagaatcgcc
     2941 cagatcatga ttgccttcac taccaacgcc ttcacatgtt tcattttctg cactcctttc
     3001 tcctggataa aaaagtaaga ttatctaaca agataacctt ttcattatta taccatcggc
     3061 tgagagaagc gagcaaagat aaggtcctat ctaaaaatga ggaagccact tagaacattc
     3121 acgtaataat ttatccgtac agacattcga tatggtgttc ctttactttc gcatactgtt
     3181 cttttgtcag ttctttactt gtcaccagat aatcgatttc atgaaaagca atggcttgat
     3241 gcatgaattt atgtgcaatt ttttgctgtg taactaaaac ggcaagccgg tctgattgtt
     3301 ttctaaaagc ttttttgacc attgcctctc cttcatgagt ttctgttact ccttggctga
     3361 aatctagccc gcgacaactc attatcgcta aatccgcata atatgacaat gcatcgttat
     3421 aggcttttga accgttgatt gttgctcgtt tcggaacaat tgcccctccc aatacagata
     3481 cttttgccgt tgtatattca ctcaacatag cagctgtgac gatgccattc gtaatgatct
     3541 gcaaaccatc aaatcttgct aggtttctga ctaagtgtaa agaggttgta ctggaatcca
     3601 agaataagac catatcatct cgaatcaaag aagaagctgt atctgcaatc ttacgttttt
     3661 ctgtttcttc ttctaatttg gtgatcttca tcaggtaagg cacgtcaatt tttcgattgt
     3721 caataagcat ggcgccaccg cgaactctac ggatcaatcc acttttttcc agaaaaacaa
     3781 ggtcacgttt aatcgtggct tcgctataat ttactaactc gatcaaatcg gtgactcttg
     3841 caaattggtt gttttttaga taattcacaa tctcctgctg tctattctcc acttcattca
     3901 acataaaatc accccattta ctttatatag taacaggaaa aaagaggttg tgacagaagt
     3961 gattggcttc gagaaataag aagaaatttt tcgaaaaaac tacttctgaa catcgtgtgt
     4021 ggttaagagg gtatgacaag actattgtca taccctcttg accacacatt tttattttta
     4081 gtataaacta gccttgccta ctgtgttcaa aatttccatt ttatgtttga ccacttcttg
     4141 taccttttta ttagctgctg gataaattgc attcggctcg tacatcgatg gattttcact
     4201 caatatttct ctcaaagcat tgaaaaagac gcttttaaga tctgagctca aattcacttt
     4261 ggcaactccg tattttactg cttcgctgac ttctgcgtcc ggattacctg aaccgccatg
     4321 taaaacaaat ggaagatcga tttgttggtt caattctttc agtaagtcga tatttaattt
     4381 tggagttctg tttttcggat acaagccatg cgctgttcca attgctacag caagtgtatc
     4441 gattcctgtt tctttcacaa aaatcttcgc ttgatcagga tctgtataaa tgattgtttc
     4501 agcacctcct tcagcagaac cattattccc aatcgttcca agctcagcct ccactgagac
     4561 atttaccgca tgagctaacg caaccacttt tttcgtaatc tgtacattgt cgtcgaatga
     4621 tttcaaagac gcatcgatca tcacggaaga atagccgttt cgaatcgccc gtaagacatc
     4681 ttctatcgta ccgccatgat ccaagtggat aacgatcggc agtttacttg tataagcata
     4741 ggctcttact gccgcaacaa actcatcact taaataggcg atttcatcag gatgaatttc
     4801 taagattact ggtgcttcta atctctctgc ttcttcgatg attgctttca tcatatcata
     4861 gctgcaaata ttgaaggctg gtactgcaaa ttgattctct ttagccactt tcaataactc
     4921 tttcattgta tataacatta cgcttccacc tcatttgtta gattttcatt tttcgtccac
     4981 ttgtcttgtt ttttctcttt cactgttttt ttcaatagtc caagcataaa ggctgtagtg
     5041 actgtacctg atgcaattgc caagacatac ataggtaaat tgttcataac atttggaatt
     5101 agcatcaccc aaatccctcc atgaggtgcc attgaagtaa ctcctactgc catggaaatc
     5161 cctgcagcga ctgctgaacc tgtcatcaaa ctaggtatca cgcggatcgg gtcagatacc
     5221 gcaaacggta tagcaccttc tgtaatatac gaagcgccta aaatccagca tgattgacct
     5281 gaagtccgtt cttctttggt aaatttatgt ttgaataata aagtggctaa agcaatacct
     5341 aggggaggtg tcattcctgc agccatacaa gcagcaatcg gtgcatttac tccagctgac
     5401 atcaaaccga tcgagaaagt cgaagtcgat ttattgatcg gcccacccat atcagctgcc
     5461 atcatgatcc cgataagtaa tccgaacaat agcccggatg tatttcccaa gctattcagc
     5521 caatttgtca aagcatccaa cacccatgtg atcggttgtt caatcacaaa taccatcaaa
     5581 agcccaataa ttaagatcga taaaaagggg acgacaatca gctgataaat agcagaaaat
     5641 tcttttttta cgctaatttt atttgccaga aaatcacaga tataccctgc tagcacgcca
     5701 ccaatcattc cgccaagaaa acctgaacca cctgtagagg ccagtaatcc ggcaacaatt
     5761 cctggggcaa aacctgcttt tccagcaatc gatgtagcaa tccccgcgcc taacgcagga
     5821 accatcatgg caaacgctgt atctcctccg atccttgaaa atgcagctgc taacacatta
     5881 tagtctgctg agttgggatc agccgctgta atcccaaagg caaaactcag tgcgatgatg
     5941 attccgcctg cgatcacaaa tggaagcatg taattcacac cattcatgaa atgattatag
     6001 attgtcggct ttttctcatt tgcagctggt tctttttcat tagacacaac gaatacgcca
     6061 atatctctca atgcttcgtc tattactact tgcggttctt tgattgctcg gcttgtagag
     6121 acacgcttta cttttttccc ggcaaatcgg tccaattcga tttctttatc tactgcaaga
     6181 atgattgcat ctgcctcttt gatttcttgt tcagataact gattttctgt ttttacacct
     6241 tgtgtttcga attttgcttg atggtttaga tttcttgctg ttacttctaa tttttctgcc
     6301 gacatataag tatgagcaat tccagtcggg caagcagtta caccaacgat tttcatgatt
     6361 attttcctcc tttttcttca ctatcgatac tcggacaaag actctactat tttcattctt
     6421 gactaatctg tctgatgata tctgctggcg tagtggcttc ttttattccc tttctaaaat
     6481 ctgcattcat caatcgtctg gagagtgtgc tgagaatctc tagatgcata tctgctgatt
     6541 tttcaggaac tacgatggca aaaatatatt caattggcat ttcatctatc ccgctatagt
     6601 ccgttaattt ggctgtatga aaaaaacaca gtgctggttt tgtcacatat ttgctttttg
     6661 catggggaat cccaaatcca tcttctattc ctgtggagat ctcctcctct cgtttgaata
     6721 gtgccttaac gactttttga acatttctta ccttatttgt ttttttcaac acatccccta
     6781 ttcgctcaaa taatgatcct tttgaatcta tcgatggatc gaaaacaatc agttcttctg
     6841 cgatcaattg atcaaacctc ataaaaaacc tctcccccac aaaaaatgtt aacgttttct
     6901 tggaactgag gtcagtatag catcgctagc tgtaccgttc aatattatga atgaacagag
     6961 gaaaaagacc catcatccgc gtgtataagt cattaaaatg aacgatctag ctcacaaaca
     7021 atcgcaagtc tataacaaaa caaaaagcct tccttgaaag gaaagcttag cttctaattt
     7081 taggtaaatg gttttttact tcgaaataac ccctcttcat ttcgttaata taaggtgttt
     7141 ttatcaagtg gataattaaa actatatact agtttttcat tgttttgtgc attatgatcc
     7201 tacgactttt tcagcatcaa acgaagtttt aaattggcaa aaaacataaa aaaacagact
     7261 atccctatca gatagtctgc taaacgttga gttatcagcg tttttattat tccgcagcgt
     7321 ttgcgtcttt gagaccgtat tttttgttga aacggtccac acgtccgtct gcttgtgtga
     7381 atttttgacg tccagtgtag aatggatgtg agtcagaagt tacttcgaca cggatgactg
     7441 ggtatgtatt accgtcttcc cattcaactg tttcttgtga agtttttgtt gaacctgaca
     7501 agaatttgaa accagttgtt gaatccatga atactactgg gtggtaatct ggatggatgt
     7561 tttctttcat gattttttcg ctcctttgcc ctgctccatt tgctgaacca gagttgattt
     7621 gtcgatttac aacattaaca tcttaacatg tttcagtcaa ggttgcaatt atcttttcag
     7681 atttctggtc ggattttttt taccaaagga gacatcttga aattctttga aaaattgctg
     7741 attgtttttt gttttacgca aaaagcggat cagttgttcc gtatattcta atgaatctcc
     7801 agtcatattt tttcgaagtt tccaaatttc ttctaattct tcacttggca tcaataattc
     7861 ttcttttcgg gtacctgatt ttttgatatc gatggctggg aagatccgac gttcagataa
     7921 ttcacgtgaa agttgtaatt ctagatttcc agttcctttg aattcttcat agatcacgtc
     7981 atccatacgg cttcctgtat cgactaaagc ggtcgctaaa atcgtcaaac tgccaccttc
     8041 ttcgatgttt cttgctgccc cgaaaaattt cttaggttta tacaatgcgg ctgggtcaat
     8101 cccgccactc aatgtacgtc cgcttggtgg aacgactaag ttataggccc gagcaagtct
     8161 agtaatacta tccatcaaga taacgacatc tcgtttatct tcgactaaac gcattgcgcg
     8221 ttccaatact aattcagaaa ctcgagtatg gttttgcggc tgcagatcaa atgtagaaga
     8281 taccacgtcg cctttcacgc ttcgttctag atccgttact tcttccggtc gttcatcaat
     8341 cagtagaacg attaattcta cttccggata gttttcggtg ataccatttg cgatttcttt
     8401 taatacagaa gttttccctg cttttggcgg tgcgacgatc aatccacgct gaccaaaacc
     8461 gattggagaa aagatatcga tcattcgggt agatagtctt tgcggtgttg tttccagttg
     8521 aagctgtctt tgcggataaa gaggtgttaa ggctggaaaa tgcggacgtt cttttgcttc
     8581 ttccggatct tttccattaa cgctttccac gtgcatcaaa ccataataac gttccgattc
     8641 tttaggtgga cgggcttttc ctgccacttt gtccccattt ctcaaactga atcgtctgat
     8701 ttgtgaggca gaaatataga tatcttctgc acttggtcca taattgattg gccgtaaaaa
     8761 accgtaacca tcttgaccaa cgatatccaa tattccttcc atataataaa atccttgttt
     8821 ctctgcttgt gcacgaatca cagctagaga taattccttt ttattcatct tactgtaata
     8881 aggaatttta aattctttgg cataactata aacttctttt aacgttttgt tctctagttc
     8941 tgccattgtc aaataatcgc tcattcagct cccacttcct ctatcatttc aattgatgcc
     9001 cctaaggctg ttagtttctc gataatatga tcataaccgc gtaagatata atctacattg
     9061 taaatcgtcg ttgtcccttc tgccaatagt ccagcaatca ctaaacaagc acctgcccga
     9121 agatcagaag ccacgacttc tgtgccgtga agtttatttg gaccattcac gatgatcata
     9181 tttccttcca cggtaatatc agcgcccatt cgtgctagtt ccggcacatg gttcacacgt
     9241 ttcgcataga tcgtatcgat gatttctgaa gtgcctgtag tcatcaatag taacgcagta
     9301 agtggctgct gcagatctgt ggcaaatcct ggataaggat aggtcatgat gtttgcgggt
     9361 tttaattctt tcgaggggta aacttcgatt tcatcttcgc tgattttcat atggacacca
     9421 atttcctgca attttgctat aaagctttcc aaatgttcaa agatcacatt tttcactttg
     9481 ataccatttc ctactgcagc tgctaatgct agataggttc ctgcttcgat gcggtcagga
     9541 atcatgaaat gccgacagcc atgtaatgtt tcgactcctt cgatacggat cacatctgta
     9601 cccgctcctc gaacttttgc ccccatatta ttcagtaaag ttgccacatc tatgatttcc
     9661 ggttctcgtg cagcgttttc aattactgtc tgtccttttg cttttacagc agctaacatc
     9721 acattgatcg ttgctccgac tgaaaccacg tccatgaaaa tccgattgcc acgcaatcct
     9781 ttattttctg tccgtaaata catggcacca tgttcatttg tcacttcagc acctagagct
     9841 tcaaatcctt tgacgtgtag gtcaatcggt ctagggccta aataacagcc acctggcagg
     9901 cctactaccg cttcgccaaa tttaccaagc agtgttccca taaaataata agaagcacgc
     9961 aaactattga tttttccgtg cggcatcgga atcgatacga cttctcgagg atcgatttct
    10021 agtatgttgt tggaaaaatg gacagttgcg cccatgatct ctaaaatttc aattaatgaa
    10081 tgaacatctt gtatgtccgg cacaccttcc aagatcactg gggaatcagc taagattgca
    10141 gctggaatca atgcgactgc actatttttt gcaccgctga tcgttacttc accagctaaa
    10201 ggtcggtttc ctttgattac gatttttttc atctgtttcc accttgttta ttaatctttt
    10261 ttcatggata tgcttgtatt ttatcataga taaaaaaaag aaacgagcat ggtctgccgt
    10321 tttaagtaaa aaagaatgga gagcgacttc aaaagtcgtt ctccattcct atatatcgta
    10381 tctgttatgt ggatgcttac gctttgttag ctgaaccgaa ccagtcgata tgagatttag
    10441 catcttcaac gattgctgct ttacctggag ctaataattt acgagggtcg aatcctttac
    10501 cttctttatc tttaccagct tcgatatatt tacgagtagc tgcagcaaat gataattgga
    10561 attcagtgtt aacatttact tttgagatac ccattgagat agctttttcg atttgatctt
    10621 gaggaatacc tgatccgcca tgtaatacta aaggcatgtc tggacctaca gcatcagcaa
    10681 ttgcttgtaa gtggtcaaaa gctaatcctt tccagttttc tggatattga ccatggatgt
    10741 taccgatacc gcaagctaag tagtcgatac ctgtagcaac catttgtttg cattcttcaa
    10801 tatcagctaa ttcgccgtta ccgatgattc cgtcttcttc tccgccgatt gaaccaactt
    10861 cacattcaac tgagatacct tttgcatgtg ctttttcaac aacgtcttta gctaatttca
    10921 agttttcttc aaatggtaga tgtgaaccat cgaacattac tgaagtatag cctaattcga
    10981 tacattccaa agcagcttcg taatcaccat ggtctaagtg gatagcaact ggaacagtga
    11041 tgttcattgc ttcaactaaa tcagtgatta aatctttaca gattttgtaa ccacccatgt
    11101 atttagctgc acccattgat gtttggataa gtactggtgc ttgttttgct tcagccgctt
    11161 caaggattgc ttgagtccat tccaagttgt ttgtgttgaa tccgccgaca gcgtaaccgc
    11221 ctttacgagc agcttttaaa aattcagctc ctgatactac tggcataata aaattcctcc
    11281 taagttttgt aaaactaaat ttgttaagac aattcttaac caacgaccct attttaccaa
    11341 acatttcggt agttttctac taaaatcaat gaaaatgaaa attttcaaga agttttttgc
    11401 aaaaaataaa atatccaata gcgctttaat cgaaaaaagt cttttgcttt ctatcttttt
    11461 tcctacaaat aataacaaat cagaaaaaga gaaaagtgac aagaaaattg cttatcccct
    11521 tctcttgcta cttttctctt tctatcaagt tcaaaaatta ttttgtttcg tgatctgcta
    11581 atgatgctcc gacaaatgct ttgattaatt tttgcgggcg atttggacga gaaatcaatt
    11641 ctgggtggaa ttggcatccc acaaagaatt ttttctctgg cagctctacg atttctacta
    11701 aacggttatc tggtgagaca cctgaaaaga ctagcccttg ttcttcaaac aattgacgat
    11761 atttgttatt gaactcatag cggtgacggt gacgttcttg cacaacatcg gctccatcat
    11821 atgcttcagc cgtcttcgtt ccttttttga ttttacatgg gtataatcca aggcgtaaag
    11881 ttccacccaa attctcgatg ttttcctgat cagccattaa atcaataata ttgtttgctg
    11941 tatctggatt cgtttcagct gatgacgcat cttctaatcc aacgacgtta cgtgcaaatt
    12001 cgacacaagc catctgcata ccaagacaaa tacctaaaaa cggaacgtca ttttcgcgag
    12061 cataacgaat cgcttcgatt ttcccttcta ctccgcgatc accaaatcca ccaggaacca
    12121 agattccatc tgcgtctttc aaacgttctt ctacattttc tctcgttaat tcttgtgctt
    12181 tgatccaatc gatctcgatg tctgaattat aggcgaaacc agaatgtttc aatgcttcaa
    12241 ctactgagat atatgcatct ggtaactcga catatttacc gacaagcgca atccgtactt
    12301 ttttcttcaa gttcaagaca cgttcttcta attctttcca ttctgtcatg tctgcttctg
    12361 gtgtatctag ttttaaatgg tcgcaaacaa tttgatccat tccttgtgct tgtaaaacta
    12421 atgggatgga gtaaagtgta tctacatctg gcgactcgat aactgcttct ggttcgacat
    12481 cacaaaattg tgccaatttg tttttcacgc tttgagaaac aggttgctct gtacgtacca
    12541 ccaaaatatt cggttggata cctaaaccac gtagctcttt tacactgtgc tgcgttggtt
    12601 tagttttcat ttctccagca gctttcaagt aaggaatcaa tgttgtatgg atatacataa
    12661 cattatctgt accgacatct gctttcattt gacgaagtgc ttcaaggaat ggcaaagatt
    12721 ctatatcccc tactgttccc cctacttctg taatgatcac atctgaatca gtcattttag
    12781 ctgctcgcat gattttttct ttgatctcat ttgtaatatg aggaataact tgaacagttg
    12841 ctcccaagta ttcgccctta cgttctttac gcaaaacttc agaatagatt tttcctgtcg
    12901 tcacgttgga gtatttgttc aaattgatgt caataaaacg ttcgtagtgg cccagatcca
    12961 aatctgtttc agcaccatca tctgttacga atacttctcc gtgctgatat gggctcatcg
    13021 tccccggatc cacgttgatg tatggatcaa atttttggat agttactttt aagccgcggt
    13081 ttttcaataa gcgtcctaaa gaagctgcaa caatcccttt tccgattgat gaaacgacac
    13141 cgccggtcac aaaaatatat tttgtcataa gttcaaaaac tcctttttaa tttatgtgca
    13201 ggaaaagtct cagacaataa acaaagctcc ctattcttca ataagaatag ggagccgaaa
    13261 tttctgtaaa cttctgaccc tctaaaaggg tgcccaaggt gtataataca agctgactaa
    13321 gggaaagtca agtactcaaa tgaatactat ccgcttacaa ttttttcttt ttcgatatcc
    13381 tttgagaaat tatttcttaa gtcctttatg agtgcaaaaa aaatttaacc cacataataa
    13441 gcttgatata aacatagcta acgttaaacc taagcaaatc agaaaatcgt tactgaaaaa
    13501 ttttaagtga aataatagat aaaaaaataa aacaaacatt atgatagata atatcccacc
    13561 taacaaagaa ctactttttc ctacttgcag caacagttta tatagcttat cgtagaaaca
    13621 atctttttct ttcaccctta tttacctcct ttacgtaaaa aacgtagtcc acagttctcg
    13681 gatttttggc atatcttttc tattaaataa tatcaaaaac aaaacaattc cactaatttt
    13741 ttcaaagatg accttttttt caacaaaagt gccttgtagg gattcattga aggtaacgaa
    13801 tcacataagt aaaaaataag gagatgcgac aaaagtcgtg cctccttatt tctaaataaa
    13861 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccatcaaac atgagaagct
    13921 gtttttgaaa ttatttcttg tgattcggag ttaaatggta ggctcacaac ctctggataa
    13981 acggtattca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc
    14041 cattttcaca aaatttttct taatactcga aactaaacgt ctttttcaaa acctcttggt
    14101 aaacggtgtt caaaagcttg catctgcggt aataagttca gccaattcaa aaacaagaaa
    14161 agctgttttt gcttggctat tcttattact tgttgcaggg cagctttttc acaacctctc
    14221 cactaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga
    14281 ggaactattt ttccgttagt ttctctaata ctcaggtcaa aacgctgatt tcacagcctc
    14341 taattcacgg tattcctgtt ttaataaagt tctagatatt gttctctttc ccactcggaa
    14401 acagtttgtc tgaatgccgc ccattcgaga cgttttgctt caacaaaatt tgagaagata
    14461 tgttgtccta atgcgtcaat cattacatta tccttacgca attctttgat agcattatgg
    14521 atagttgaag gtaagtcctg gatatgtgca gcttctcttt cttcctcatc cattacataa
    14581 atgttacggt caacagctgc tggcggtgtg atctgattac ggataccgtc tagtcctgct
    14641 tgcaacaata ctgccattgc caagtatggg tttgcagatg gatcaactga ccgcaattcc
    14701 aaacgtgtgg ataaaccgcg agattctggt acacgtacta atggcgaacg attacggcca
    14761 ctccatgcga cataaacagg cgcttcataa cctggaacga gacgtttata agaattgact
    14821 gttggattgc aaacggctgt gtaggcacga gcatgtttca gcaaaccacc taagaaatga
    14881 taagctgttt cacttagttc caattcaccg tctttatcat agaaaacatt tccttcatca
    14941 ttgaacaaag acatattgca atgcatacca gaaccattga tcccaaataa cggtttcggc
    15001 atgaaagtcg cgtgaagtcc atgcttgcgc gcaatcgttt tgacaacgag cttgaaggtt
    15061 tggatattat cacaagcatc taccacatct gcatatttaa agtcaatttc atgctgtcct
    15121 ggcgctactt catgatgtga agcttccact tcaaagccta gactttctaa ttccaacacg
    15181 atgtcacggc ggcaattttc acctaaatct gttggtgcaa aatcaaaata accaccttta
    15241 tcattcaagg tcgttgtgat cccaccgtct tcgtctaatt taaataagaa gaattctggt
    15301 tcaggtccta aattgaaaga ggtaaaacca aggtcgcgca tatctttcaa tgctcgtttc
    15361 aaattcccgc gaggatcgcc ggcaaatggt gtcccatccg gattatagat atcacaaatt
    15421 aaacgagcaa cttttccatg agcactttcc cacgggaaga tcatccaagt ggagagatct
    15481 ggatacaaat acatatcgct ttcttcaatt cgaacaaatc cttcaatcga agaaccgtca
    15541 aacatcattt tattttctaa tactttgtcg atctggctca ctggaacttc tacatttttg
    15601 atcgtaccta agatgtctgt gaacatcaat cgtaaaaatc gtacgttttc ttcctcaatg
    15661 attcgtttga tttcttcacc tgttatctgt ttctttacca tggtatccct cttccttttt
    15721 taatttattc agtcaaagaa ttgtctttgc aatccttgcg actgataatg acctgttttg
    15781 cttaacccac cttgcgccaa aatctcatca cgaaaaattt gtcgaacgtc atgatctgtc
    15841 aacggcttct tgttttttgc ttcttgtttt ttcgcctgtg ccatttcgta tacacgtttg
    15901 atcccagcca tattcaatcc ctctgaaaga tagtctttaa tctcaagcaa catatcaata
    15961 tcattcagcg aatacatacg tcggtttcct tcgcttcgtt ccggatgaac taaatcttgt
    16021 tcttcataat agcgaatttg ccgagcagac aaatcagtca gcttcataac tgtaccaatt
    16081 ggaaatacgg acatagatcg gcgcaattcc ttttctccca tcagacgtcc cctttcgttt
    16141 tataagttgg ctaaagtgta ccaattaaaa aaaatattgt caactgcata tgttaggttt
    16201 tctaacatga gatctctatt ttttataaaa taacaaaaaa ataagaacac aaaaaaggta
    16261 tctagttgat tttcgtacca gatacctttt attttttatt atactgtact atcctttatt
    16321 agtgttttta tt
//
LOCUS       DAFFFQ010000068        15950 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000068
VERSION     DAFFFQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.068"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            31..1062
                     /locus_tag="LQB69_002189"
     CDS             31..1062
                     /locus_tag="LQB69_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL3526333.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            1059..1826
                     /locus_tag="LQB69_002190"
     CDS             1059..1826
                     /locus_tag="LQB69_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL3526334.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1926..3494
                     /gene="liaX"
                     /locus_tag="LQB69_002191"
     CDS             1926..3494
                     /gene="liaX"
                     /locus_tag="LQB69_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL3526335.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3791..4111
                     /locus_tag="LQB69_002192"
     CDS             3791..4111
                     /locus_tag="LQB69_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL3526336.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4111..4461
                     /locus_tag="LQB69_002193"
     CDS             4111..4461
                     /locus_tag="LQB69_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL3526337.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4628..5566
                     /locus_tag="LQB69_002194"
     CDS             4628..5566
                     /locus_tag="LQB69_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL3526338.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5581..6411
                     /locus_tag="LQB69_002195"
     CDS             5581..6411
                     /locus_tag="LQB69_002195"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL3526339.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6455..7480
                     /locus_tag="LQB69_002196"
     CDS             6455..7480
                     /locus_tag="LQB69_002196"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL3526340.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7495..8433
                     /gene="galU"
                     /locus_tag="LQB69_002197"
     CDS             7495..8433
                     /gene="galU"
                     /locus_tag="LQB69_002197"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL3526341.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8558..8998
                     /locus_tag="LQB69_002198"
     CDS             8558..8998
                     /locus_tag="LQB69_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL3526342.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            9001..9603
                     /locus_tag="LQB69_002199"
     CDS             9001..9603
                     /locus_tag="LQB69_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL3526343.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9662..10768)
                     /locus_tag="LQB69_002200"
     CDS             complement(9662..10768)
                     /locus_tag="LQB69_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL3526344.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10978..11979
                     /gene="ccpA"
                     /locus_tag="LQB69_002201"
     CDS             10978..11979
                     /gene="ccpA"
                     /locus_tag="LQB69_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL3526345.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(12316..14661)
                     /locus_tag="LQB69_002202"
     CDS             complement(12316..14661)
                     /locus_tag="LQB69_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL3526346.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            14940..15842
                     /locus_tag="LQB69_002203"
     CDS             14940..15842
                     /locus_tag="LQB69_002203"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL3526347.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
BASE COUNT         5119 a         2823 c         3327 g         4681 t
ORIGIN      
        1 cttttcatca tttcatagaa aggataaaga atgcaaaaaa aaattagcat catcactata
       61 ttttttattt gtgtgcttac ttcatgggtt attcagcatc aagccgcttt atatgaagta
      121 cctgtcatcg agatagaaaa agtacgaata aaagaggatc atcaattgat tacaggaaaa
      181 ctgatgaaca cggatcaaaa aggagaatcg atttatttat cggttcctta tcaaaaaaat
      241 caaatagaaa cagcaaatat aaaagcgaga caaatgtatc tagtccaaat gcatggagat
      301 gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt tcttcttcac aagtatcttc
      361 attggcgcca tgttgcttat tggtggaaaa cagggtctat ttgctttagg aagtctcatc
      421 atcaatatcg ttttactttt acttttgttg ctcatcttta ctaaaacaga agggatttct
      481 ctattaaata tatctatcct gttcgtcgtt atagggatga ttattagcat attggcttta
      541 gatggatgga atagaagcag tataatcaaa atttcagctg cagttacgag tgttttttta
      601 gcatttttta tttgtctttt gacaatgaat cattggaaag accaaggact gcgctatgag
      661 gaactgaatt atctgacacg accgtatcgt tctgtttttc tttctagcgt actgattgga
      721 acagctggag gaacactcga taccgttatc acagtcattg cgacattaga agaattgact
      781 aagcgacaac cagaaataac agcgaaacag ttatggcttt caggaaggca agtgggtcga
      841 gatgtgattg gctcgatgac caatatcctt ttgttcgtct atatcagtgg gtcagttcca
      901 tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag aaacgataga acagcattta
      961 tcattggaat ttttacgcgt cattgctggc agcttaggaa tagtattatc gatccctatc
     1021 tcggtcctct tttttctgac tgtaaggagg ctgaaacgat gaatgtactt attatacttg
     1081 ttgggatttt agcaatcagc gtgctactta ttggcggtac gcaaggtatg tatattcttt
     1141 taggcttatt catcaattta gggatctttt tcctgttgtt attcggttat catcagaagt
     1201 ggccgatact ggcattgtcg gtcataggat ttttattgat tgctgtggtt atcttatttt
     1261 ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt gagtatactg atctttttat
     1321 tttgtttcct actattgctt cctatcactg attttttagc aatccaaggt tttacatcgg
     1381 tagaactaga agaattatct ggcttagata agactttagc gattgatttt agattactta
     1441 cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt agatgcgagt gtagcaattt
     1501 ctagtgggac attcgaagta tatcaggcaa atccgcaatt atctttttac caattgcgcc
     1561 attctagttt cgctattgct aaaaaaatat tggcgtcaac agtaatgaca cttctttttg
     1621 cattcatggg aagttcttta gcgttgatcc tctggtttat tgatttagac attccttttc
     1681 agcaagtgat caatgaaaaa tcatttgttc tagaatatac gatggctatt cttaccacat
     1741 tgtccgcact acttgttctt cctttaacct gcgtgactac agcatatctc tttacaaaaa
     1801 agaaagaaca actcaagatg gagtaaaagt tacaacttaa gtcctatgac atttttactg
     1861 aatcagtgca taatatggat gtaaggaaaa gcttgcaaac aattttgctt aaggaggcaa
     1921 tttccatgaa agaaagagaa cgtattttag atttagtgaa aaaaggaatc ctttcgactg
     1981 aagaaggctt agatttatta gaaagcatgg caactgaaaa agacgaaaag caaatcaaaa
     2041 aagaagcaga caaggtaact gctagtcata aagaaaaaga tcaggcaagt caattgatcg
     2101 ataaacttga aaacggcgaa gaagaaattt ctgagccagt tgatccaaag gaaagagaaa
     2161 gacaagatca agaaaactta gaaaagatct tagatgaact agcaactgaa gcgaataaaa
     2221 cttcggcgcg tctagatgaa gtaaatgctc aattcgctga caataaggca gcacgcaatg
     2281 agaaacagga agcattgatg cagctgaata caaaagaaga attaggcgag ttgacagaag
     2341 aagaattagc acagcgtcag acgttagaag cagaaatcaa agaattagaa gctagtggcg
     2401 atacactgtt agaagaacaa ttgaaattag aagcagaatt aaaagatatc cgtaagaatc
     2461 aatggtctga gaaaaaagaa acgtttactg ataagtttga attgccagat gattggaaag
     2521 atcaagcaac agatacactg aatcaagtag gcgaaaaaat gtcagaagct ggatcacagt
     2581 taggcaaatt cttgaagaaa acattccaaa ctgtttcaga aacagtgaat gacaacatgg
     2641 aatggaaaga tgtcagctta cgtgtccctg ggattgcgac aacgaaattt gagcatgaat
     2701 tttactatga agctcctgca gcaagtatcc ttgatatcaa ggcagcaaac ggaaacgtga
     2761 cgttaaaaac gtgggacagc aacgatgtga aagttgaagc taagatcaaa ctttacggaa
     2821 aaatgggtgc tgaaccattt gaagcattct ctgaacggag ccagattgag gttaatgaag
     2881 atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc agaccttgtt ttctacttac
     2941 ctaaacgagt atacgaccac gcagctatta aacttctaaa tggcaacatc atgattgaaa
     3001 cattggaagc taaagatatt tatacaaaat ctacgaatgg taacatcatc gtggaccaat
     3061 taactgcaac gatgctggaa gtagaaggtg taaacggcaa tatcgatatt cgaaacggga
     3121 acatcttaga ttctattatc gaaacagtaa atggtacagt gacatttggt gcgacagcag
     3181 aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct tacgattaaa gaagataatt
     3241 tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa agtcgcttta ccagatggca
     3301 tcggtttgga aggacatgct aaaacaagtc ttggaagtat caacagccgt ctgtccaatt
     3361 acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat gttgcaattc cgtcgtgttt
     3421 cagatggtga aatcgcgcaa gtgcaacttt caacgacaac aggaagtatc tatttaaaag
     3481 atacagataa ataaagaggt tgtgaaagag atgttcagcc ttgagcaata agaagcgatt
     3541 ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta ttgccgatag tctatctctt
     3601 gaacaccgtt taccaagaga ttatgaacct gccaattatc gccgagtcac aaggaataat
     3661 ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt gtgaccgaga attttgcaga
     3721 aagacaccga ttaagaagaa ggttgtcaca acgattttct tcatttaaaa tagtagaaca
     3781 gaggtgagac atgatgaaaa aaaagttgac aaaatctaat aaaaatgttg ttttgacagg
     3841 gactttagca ggaataggag aatacatcgg gattgatccg actgttatcc gtgtgcttta
     3901 tgtattcctg agcttagttg cttttggaag tccaattatt ttatacattt tattagcttt
     3961 gattatccca tctgcgcgtt ctgcacgaac aaactatgga cataacaatg catattatga
     4021 aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa aaacgcaaag aagcggaaaa
     4081 aatcgatgat gacgaatgga gtgactttta atgtcgtact ttcaacgtat tttagttaat
     4141 acgttaacat tcatatcatt ggctgttctt tttcctgcga tggtccatgt gagcagtatt
     4201 tggacggccg ttcttgccag cttcgtattg tctgtgttga atatgtttgt caaaccgatt
     4261 ttgacgatac tgtccctgcc ctttacatta gtcacattgg gattgtttac gtttgttatc
     4321 aatgcagcga ttcttaaaat gacctccttt tttgtgggag cagaacattt tagtttttct
     4381 tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc ttgtcaatgt aattgtcagc
     4441 gaacataaca gtagaaaata aaagaaagct gtgccgaact atcggcgcag tttttttatt
     4501 tagaatcagg aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt
     4561 ggtagtactt ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt
     4621 agcatgaatg tcagaagttg taaaagttag ccaattagtg aaaaaattat ctttggaaat
     4681 cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta actggggaca tctcacgccc
     4741 aggactagag ttgactggtt attttaatta ttattcccat aatcgtttac agctatttgg
     4801 cagtaaagaa atcagttttg cggaacggat gatcccagaa gagcgtttga tcatcatgag
     4861 aagattatgt agtgaagata tgcccgcatt tattatttca agaggattac cagctccaga
     4921 agaaatgatc caggcagcaa atgaaaatgg gatagctgta ctgcgttctc ccatctcaac
     4981 ttctcgttta ttaggagaat tatccagtta tttagatggt cgccttgccc ctcgaacaag
     5041 tgtccacggt gttttagtgg atgtttacgg attaggtgta ctgattcaag gtgacagtgg
     5101 gataggaaaa agtgaaacag cgttggagtt gatcaaacgc ggccatcgat tgatcgcaga
     5161 cgatcgagta gatgtttatc agcaagatga attaacggtg atcggagaac cgcctaaaat
     5221 cctggaacat ctgattgaaa tccgaggaat cgggatcatt gatgtaatga atctttttgg
     5281 tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct gtttatctgg aagcatgggc
     5341 aaaagataag aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt
     5401 gggcattcca caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga
     5461 agtagccgcg atgaatttcc gtgcacgtac aatgggcttt gacgcaacga aaacatttga
     5521 agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac gactagcaag gagtgacaat
     5581 atgttagcac aaatcaatcg agttgctttt gagcttgtag ggattcctat ctactggtat
     5641 gcattgatca tcgtatcagg tatcattatc gctatgtggc ttagcagcag agaggctgtt
     5701 cgggtaggat taaaagcaga tgatgtgaca gattttatgt tgtggggatt gcctatctcc
     5761 attattggcg ctcgcttata ttatgtttta tttgatcttc cccagtatat agctgatcca
     5821 atccaaatat tcaatatccg ttcaggaggc ttagctattt atggcggact gattgcaggc
     5881 gcgatcacgc tgtatttttt tactaaatat cattttattt ctttgtggac ttttttagat
     5941 atcgctgcac caagtgtatt attagcacag gcaatcggtc gttggggaaa tttcatgaac
     6001 cacgaagcat atggaccaga cacgacgcga agcttcttag aaagtctgca tttaccgcaa
     6061 ttcatcattg ataatatgta tatcgaaggg atctatcgtc aaccaacatt tctttatgaa
     6121 tcactttgga gtttgattgg atttattttg cttttgtttt tacgaaagaa accacacctt
     6181 ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt atagtttcgg gcgtttcttt
     6241 attgaaggat tacgtacaga cagtctttac tttttaggtg tgatccgtat ttctcaatta
     6301 ttatctgctg tgttatttgt cggtacattg attctattta tttggcgcag aaaaaaagga
     6361 aatttaccgg attatgaccg ctcattagga aaaaatcaac aaattatctg atttttagag
     6421 cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg
     6481 accaggctct tggggtactg ctttggcaca agtcctcgcc gaaaacggac atgacgttcg
     6541 gatttgggga catcgtgctt cacaagtaga agaaatcaac acccaacaca cgaatcaacg
     6601 ttatttacca gatcattatc tacccgattc catccaagca ttcaccaata tggaagatgc
     6661 agtaaaagac gcagatgcgg tgctattcgt cattccaacg aaagcaatac gttctgttgc
     6721 aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc atccatgcaa gtaaaggatt
     6781 agaacaggga acacataaaa ggatctctga catcttgtta gaggaaatcc cagcagaaaa
     6841 aagaaaagct gttgttgttc tttctggacc aagccatgca gaagaagtag caaaacatga
     6901 catcactacg atcacagctg caagtacaga tgcaaaagca gcaaaatatg tacaagagct
     6961 gttcatgaat aattattttc ggatctatac caatcctgat gtgatcggtg tagagatggg
     7021 ggcagctttg aaaaatatta ttgctattgg tgctggcgct attcacggct tagggtttgg
     7081 cgataatgca aaggcagcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt
     7141 agcaatgggt gccaacccgc tgacctttat cggattaagc ggaataggcg atctagtcgt
     7201 tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc aacttgttag gacaagggca
     7261 gcccctagaa gaggtgctag ccaatatggg aatggtcgtt gaaggtgtga atactaccca
     7321 agcagcgatg gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta
     7381 tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga
     7441 tggaaaaaaa gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa
     7501 gttaaaaaag cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaaa
     7561 gctatggcaa aggaaatgtt gccaattgtt gataaaccaa caatccagtt catcgttgaa
     7621 gaagcgttga aatcaggtat cgaagacatc ttgatcgtta caggaaaagc taaacgacca
     7681 atcgaagatc actttgattc aaatattgaa ttagaaacaa acctaagtga aaaaggaaag
     7741 accgaattat tgaaattagt agaagaaacg acagatgtca acttgcattt catccgtcaa
     7801 tctcatccaa agggtttagg acatgccgtt cttcaagcaa gagcctttat tggaaatgaa
     7861 ccatttgtcg ttatgctagg cgatgatata atggaagacg aagtaccatt gacacagcaa
     7921 ttaatcgatg attacgaacg tactcatgct tctacgattg ccgttatgca agttcctcat
     7981 gatgaaactt caaaatacgg aattatcaat ccaggagaag tattggaaga tgggttatac
     8041 aatgtcaaaa actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt
     8101 attggacgtt acttattgac tccagaaatt tttgatgtat tgtccgagca acaaccagga
     8161 gccggaaatg aaatccaact aactgacgca atcgatacat tgaataaaac acaacgcgta
     8221 tttgcccgta aattcacagg taaacgttac gatgtcggtg acaaatttgg ttttatgaaa
     8281 acaagtattg aatatggtct aacacatcca gaagtaggtg cacctttacg tgagtacatc
     8341 ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga tcgatcataa tccaaaagga
     8401 ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt ttctaacgag cttcagctgc
     8461 taattgaata aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta
     8521 tccttataga aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg
     8581 attgatcgca gcaatcgcct ttgcgatact tgtcgttttt atcgttcgtg tgttgttgca
     8641 agtttctaaa actttagaaa aagtagatca gactgtagca gaagcaaata cgacgatcga
     8701 aatcgtcaca aaagacgtag atcttttgtc tagacaagta gaaggcttac tggtcaaaag
     8761 taatgaactg ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc
     8821 agttgctgat ttaagtgaaa gtgtatcaga acttaatcat tctagtaaaa acatcgcgac
     8881 gaaagtcggc ggaatcggta aaacgacagc taaagcgacg gttgccggaa aagtcggtgg
     8941 gacagcgatg aaatttttta aaaacaaaaa acatactgaa acgacaggag gaaaataaaa
     9001 atggctaaaa aaggtggatt tttattaggt gcagtagtcg gaggcacagc cgcagcaatc
     9061 gccgcgttat tgttagcacc aaaatctggt aaagaacttc gggatgaatt agctgcccaa
     9121 gcggatgatt tgatggatat ggcttcagat tacacagata tggcgaaaga aaaatcgact
     9181 gagttaacag gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggtgatcta
     9241 gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg aagcaggaag tgaatctgac
     9301 gatatcttgt taaatcttaa agaacaatca gctgacttat ctaatcgttt caaaaaaacg
     9361 gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta ttgctaaaga tacttcagaa
     9421 gatatcatga ttgatgtaaa agacgctgct ggtaaagcag gagatactgt caatgaagga
     9481 atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa tgatgaaaat ggcaaaagaa
     9541 gatgcgaaag aagctaaaaa agaaataaaa gatacaatga cagattcatc tacaaatcac
     9601 taaaatcaca aatttataga ataaaaaggt gggagcggac tagctctcac cttttttact
     9661 cttatttttc taaaacaagt aattcttttg gtgtagtcgt gaatggcaca cagccatcgc
     9721 ttgtcacata tacacagtct tcgatacgta cccctacttt ttcaggaata tagattcctg
     9781 gttcaagaga gaagcacatc ccttcttcga ttactaagtc atttccttct acaagggaag
     9841 ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg gtgattgaaa tattcgccgt
     9901 agccatagct gttgatcacg tttcgtgcga tttgatcaag ttcacctgct gtcacacctg
     9961 gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt gatgttgtag attttttctt
    10021 ggaagtcggt tggtttttga taagcaattg tacgagtagc atcactgcaa tagccattcc
    10081 aaacaacgcc aagatcgaat aacacaagtt cattcggtga aatcttcgta tttcctggtg
    10141 taccatgagg gcttgcagca tttgctcctg caaggactaa tgtgtcgaaa gacatttgtg
    10201 aaacaccttg ttttttcaat tggtattcaa tttctgcaat gatcgcttgt tcagtcacgc
    10261 cttctttcac tgctttaaag ccaatctcaa atgcaacatc ggcccattta ccagcttcga
    10321 tcagtttgtc tttttcctct agagttttga tcagttgtag acgttggatg atggaagtta
    10381 gatcagtgta agaatccgtt cccgtcaaga tatcagagat agcatcgaag cgctcgacag
    10441 tcaaggcgtt cttttcaatc cctacattgc caggctgacc atagcgagca cgaagttctt
    10501 ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc acggacatca tacgcccaac
    10561 tactgttttg agcatcttca acttccaaag caggagtgaa aaggaaagga tcttgttcaa
    10621 gagggataaa aagagctagt acacgctcat gaggatcact ttcgtatccg gaaaagtaag
    10681 cgatgtggcc agggtcgctg atataagcca attcgacttg gtgttgtgcc atccattgac
    10741 gtagttcatt gatttttacg ttattcattt ggcaacctac tttctaaatt aattttgtat
    10801 tcaataattg taccactgat ttattaaaag accaacgttc taaaaactga aaaaagtaaa
    10861 aattttcatg aaaacagaga taaaacggtt gcaattcttg agattatttg atattctatt
    10921 ttagaaatgt aagacgtatt ttcagaaaac atataaaata tgaataggag aacacaaatg
    10981 gaaaagcaaa caatcactat ctatgatgta gcgagagagg caaatgtttc gatggctact
    11041 gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag caacaagaaa aaaagtcttg
    11101 gaagttatcg atcgcttgga ttatcgtcca aacgcagttg cgcgtggctt ggcaagtaag
    11161 aaaacaacaa cagttggtgt aatcatccct gatgtcagca atatgttctt tgcttcttta
    11221 gcacgtggta tcgatgatgt ggcaacaatg tacaaataca atattatctt agcaaattca
    11281 gatggcaacg accaaaaaga agtcaatgta ttgaataact tactggctaa gcaagtggac
    11341 ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc gtggagaatt ttcacgttca
    11401 aaaactcctg ttgtattagc tggctctatt gatccagatg aacaagtagg tagcgtaaac
    11461 attgattatg tatccgcaac aaaagatgca gtaaacaaat tggcaaaaag cggcaataaa
    11521 aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca atggacaaaa tcgtctgaaa
    11581 ggctataaag aagcattaag cgaaaataac ctaccatata gcgaaggatt gatctttgaa
    11641 gcacaataca acttcaaaga tggtctatct ctagtcgatc ggatccataa tagcggtgca
    11701 acagctgtct atgtttcaga tgatgaatta gccatcggta ttttggatgg tttattagac
    11761 cgtggcgtga aagtaccgga agaattcgaa atcatcacaa gtaacaattc actactgaca
    11821 gaagtagctc gtcctcgttt aacaagtatc acacaaccat tatacgattt aggggcggta
    11881 tctatgcgcc tattgacgaa aatgatgaat aaagaagaag tcgaagaaaa aacgattatc
    11941 ttgccttatg gaatcgaaga aaaaggatct acaaaataat aaaaggacaa ccattcagag
    12001 gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa tttcaaaaaa cagcatctca
    12061 tgtttgatga atattgtgac tcgtgattcg gagttggcag gagaacaccg tttatttggg
    12121 aaaaggggtt atgacaaaat tttgttataa tccttttttt gtgtcttaaa aaagagagaa
    12181 agtggttgaa attacattta tcgtatataa aacaatccgt gtatagatgc tgcattttgt
    12241 tcaccaccat caaaaaaata agggactgtg acaatactat tgtcacaatc ccttattact
    12301 gtgtattcta ttaaatcaat catctttctc atctgattct ttagttgctt gattcgctct
    12361 tgcataagct gaagcggtgt tccagtaatc tgtatagttt tcatcggtac cgccaacgcc
    12421 aaatttaaag gcgcttttct tcggtccatt tttcgcccat agcgattcaa ctgtttttcc
    12481 tggtgtttgg atggattttt tattgacagt aacttttcca ggtgtttgac cagtaaatcc
    12541 agatactttt ttcttcatca catcagatga taattcgaat ttctcatcag tgccaaaaat
    12601 gtcaggtttt gcttggtaag cttgattgat cagattggca agataattag ccgaacgtat
    12661 ccttgctttg ctgtccattc cagtattgtc atcgtgccct gcccagctac tgatggtaat
    12721 agacggagta gagacaatta gccatgaatc tcggtattca ttggtagaac cagtttttcc
    12781 gacccaatct gcttttccta gatttccgtt taagcttgag atggcatctt tgaatggtgt
    12841 agtgattttt gcattgatca cactacgcat catgtcattc atgatcgatg ccgttgcttc
    12901 cgaatagatc cggacagagt tgctttcgtg tttataaatt acatttcctg cgttatctgt
    12961 gatggaatcg atgatatagc cttcttcata gacgccgccg tttgctaaag cctgaaatcc
    13021 gttggtctgt tgaagcgtcg tgacatctac tgtacctaaa ggagcagatt caacgcccca
    13081 attgtcattt gccgggtagt tcatctttgc caagtagtgc tcgtaagcat attgtttcga
    13141 tccaccggaa ttcaacacat cttgatataa gtgataagcc gggatattgt tggaccattc
    13201 tagtgactca cggacggttt ggaaggtgtt cgatccttcg ttagtagcat tgacgatttt
    13261 ctcgccagca ttttctcctt cttgccaagt tgttgcataa tctgatacac gggattctga
    13321 tccgataagc cccatgtcga ttgcaggtcc ataaacaaga actggtttga ttgatgagcc
    13381 agcttgacgt tctgtatcga aggcatggtt attttgattc tgactgtaat tccgtccgcc
    13441 aacgaatccg tagatgcggc cagttctgtt atccatcaat acatttccgg tttctacttg
    13501 actgccagca ccatcatcta acatgtatcc ataattttca acgccatttt gcaatgcagc
    13561 atagacatct ttatcgattg tcgaatggat tgtatatcct ttattttgga tcgtttgctg
    13621 tgccttttga taataagcgt cactgaccga tgaatcagac atatcagcgt tgtctttttc
    13681 agccaattgt tgtgcaatga ttcttgttgc ttcattcatc acagtataat acaagaattc
    13741 tctgtcagtc tgttctgcga tttgttgtgg tagaaagtct tttgtaaggt cataggcttt
    13801 tgcttcttcg tattgttctt tcgtaatttg gccttcacga tacatactga ataaaacaaa
    13861 atcttttctg gcaaggccgg cagaaagatc ttcttttagc gcgcctgtgt tcgtgtatgg
    13921 actatacgtg atcgggcttt ggggtaatcc agcgatataa gcagcttgcg gcagcgtcac
    13981 atcttttgca tctacaccaa agattccttt tgcagcttcc tggactccag caatgttttg
    14041 tcccttatta ttacgaccaa atggtgagac attcaaatac gtagcaatga tctcatcttt
    14101 tgaaaagtat ttttctactt gtgcagaaag aatgatctcg tttgctttac gtttgaatgt
    14161 tgtttcatct gtaaggatct gctgtttgac taattgctgt gtcaaagttg atccaccaga
    14221 agagccaata ccagtagctt cagatactaa tgctcgtaaa acagctttag gaacgtaacc
    14281 ttggtgttta tcgaagtatt catcctctgt gctgataatt gcagttttca ataaaggtga
    14341 aatcttatca gatgaaatag tcgtgcgcat cagatctgtc tggatttttg atatctctgt
    14401 attatctgca taaacaatct ttgacgtttc tgtaatgttt cctagttctg tctgcagtgc
    14461 ttttttagtc ggcaattgtg tgtcttcgac gagataggca aaatagccag cgcctattcc
    14521 aatacctaga gctgcgaaca tcaaaagaag aacagtaata aaaacagtca atgattgaaa
    14581 aactctaaaa ataatcttag gaacaaacca tttttctttt tttcttctat tctgtttttt
    14641 tcgagtattt ttgcgtggca aaagggtcac ctcaactaat ggttttattt cgctaattat
    14701 agcaaataat agaacaaaaa aacagacaag gctgtttttg gcaggaattt cttttttaaa
    14761 agagttaaaa cgtgagaaaa tgatctttat tataaaagag ttcttaagct tttctttaga
    14821 gaaaatttca gttactatgg agaacagggc aaagaaaaat caatcatttt ctttgtatat
    14881 catcttacca atatatagga attgttagta acaattgcct ttcaaggagg aatccatcca
    14941 tgttattagg ctcacacgtc agcatgagcg gaaagaaaat gctgttagga gcagcagaag
    15001 aagcagccag ttatggatct tcgactttta tgatctatac aggcgcccct caaaacacta
    15061 gaagaaaagc catcgaagag atgaatattg atgccggaaa agcgtacatg gcagaaaaag
    15121 gtctaacaaa tatcgtcgtt catgcccctt atatcattaa tttggggaat acgatcaaac
    15181 cagaaatgtt tccttttgcc gtccaatttc tgagagaaga gattgttcgt gcggaagctt
    15241 taggcgctac tcaaattaca cttcacccag gtgctcatgt aggggcagga acagaagccg
    15301 gaattgctag aattattgaa ggcttgaatg aagttttgac aaaagaacag acggcacaaa
    15361 tcgctttgga gacaatggca ggtaaaggta cggaaatcgg tcgaacattt gaggaattag
    15421 cgcaaatcat cgatggagta aaattgaatg aaaaattatc cgttactttt gacacctgtc
    15481 atacgaatga tgccggttat aatattaaag aagacttttc aggtgtaatg gaagagtttg
    15541 ataagatcat cgggttagat cggcttaaag tactccatat caatgattct aaaaatccgc
    15601 aaggttcgca taaagaccgg cacgccaata ttggttttgg aacaatcgga ttcgacacgc
    15661 tgaataagat cgtccatgat ccaatgtttg acaatatttc gaaaatcttg gaaactccct
    15721 atgtaggcaa agataaaaag aaagcttacg caccatatgg aaaagaaatc gccatgttca
    15781 aagcgcaaac attcgatcca gaagtgtttc ctgagttatt agcagaagaa gtgatactct
    15841 aagattttct atctgacagc agcaaaaaag gaccaggcac attgccttgt ccttttttgt
    15901 ttaagaaaca aaggagtact ttttgcagtc tttttcatca tgcatcttct
//
LOCUS       DAFFFQ010000069        15471 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000069
VERSION     DAFFFQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.069"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..1126)
                     /locus_tag="LQB69_002204"
     CDS             complement(<1..1126)
                     /locus_tag="LQB69_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL3526348.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
     gene            complement(1209..1544)
                     /locus_tag="LQB69_002205"
     CDS             complement(1209..1544)
                     /locus_tag="LQB69_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL3526349.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(1554..1928)
                     /locus_tag="LQB69_002206"
     CDS             complement(1554..1928)
                     /locus_tag="LQB69_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3526350.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(1941..2255)
                     /locus_tag="LQB69_002207"
     CDS             complement(1941..2255)
                     /locus_tag="LQB69_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL3526351.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="LQB69_002208"
     CDS             complement(2368..5595)
                     /gene="fss3"
                     /locus_tag="LQB69_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL3526352.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(6009..7175)
                     /locus_tag="LQB69_002209"
     CDS             complement(6009..7175)
                     /locus_tag="LQB69_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3526353.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            7200..7526
                     /locus_tag="LQB69_002210"
     CDS             7200..7526
                     /locus_tag="LQB69_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526354.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            7679..8731
                     /locus_tag="LQB69_002211"
     CDS             7679..8731
                     /locus_tag="LQB69_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3526355.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            8807..9025
                     /locus_tag="LQB69_002212"
     CDS             8807..9025
                     /locus_tag="LQB69_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526356.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            9012..11585
                     /locus_tag="LQB69_002213"
     CDS             9012..11585
                     /locus_tag="LQB69_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526357.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKF
                     DSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSC
                     N"
     gene            11576..11773
                     /locus_tag="LQB69_002214"
     CDS             11576..11773
                     /locus_tag="LQB69_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526358.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            11777..12388
                     /gene="mobC"
                     /locus_tag="LQB69_002215"
     CDS             11777..12388
                     /gene="mobC"
                     /locus_tag="LQB69_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBL3526359.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            12373..13764
                     /locus_tag="LQB69_002216"
     CDS             12373..13764
                     /locus_tag="LQB69_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL3526360.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            13980..14294
                     /locus_tag="LQB69_002217"
     CDS             13980..14294
                     /locus_tag="LQB69_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526361.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            14287..14781
                     /locus_tag="LQB69_002218"
     CDS             14287..14781
                     /locus_tag="LQB69_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526362.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            14849..15163
                     /locus_tag="LQB69_002219"
     CDS             14849..15163
                     /locus_tag="LQB69_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526363.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            15262..>15471
                     /locus_tag="LQB69_002220"
     CDS             15262..>15471
                     /locus_tag="LQB69_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3526364.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         5204 a         2602 c         2782 g         4883 t
ORIGIN      
        1 gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc aaaatcagaa
       61 agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa atcaccgcac
      121 tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt aaaaagttgg
      181 gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt tctttcattg
      241 ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa atttcttcct
      301 tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca tttttgggat
      361 cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg aggaggaaat
      421 aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa tcataagccc
      481 atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg tctatcccaa
      541 ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct ttgagttcct
      601 tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt aaaagctgat
      661 cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct ttcattcgat
      721 aatagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc aaatctttga
      781 aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga atcatccgag
      841 ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga atataaagca
      901 atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct tttatagacc
      961 aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta aactcttgaa
     1021 aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga acaaagtgat
     1081 aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg atggtttcca
     1141 cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt tatgctagtc
     1201 atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa aaagccttca
     1261 atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg ccgacataag
     1321 ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca gtctagtaca
     1381 tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat aatcagaaac
     1441 attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc gccttttcca
     1501 tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt cacctatttc
     1561 tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa gactaaatca
     1621 tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt ggcaacggta
     1681 tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg taagggtaca
     1741 ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa attataggtt
     1801 cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac ttctcggcgt
     1861 aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat accttctgtt
     1921 aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa cacgtaatca
     1981 gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt cacggctttt
     2041 agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac cacctcaata
     2101 tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac cgtagtattc
     2161 cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt tccaaaggtt
     2221 tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt tcattgttag
     2281 ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac gttggttctt
     2341 aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat aatagcccgc
     2401 cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt tctccccggt
     2461 ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa cagttggtgt
     2521 ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta aggtttgaga
     2581 tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat cgtaattgat
     2641 ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt ttttcgcaag
     2701 cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag atttccaaat
     2761 ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag cttcttttga
     2821 gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca catcaccatg
     2881 agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa catggtgttc
     2941 cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg ctactggctt
     3001 atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga caatagtttt
     3061 atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg ttttctcttt
     3121 gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt catctttttc
     3181 ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc agtcttcttt
     3241 gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca gagaagttaa
     3301 ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt cctcaggcaa
     3361 atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca gcttctcgta
     3421 aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg taaagacata
     3481 ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt ctaatggcgt
     3541 aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc cataaggaag
     3601 attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg tggtcgcttc
     3661 atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa aggtcagatc
     3721 attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga aatcaaagcc
     3781 aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat ttttttcgct
     3841 atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat atccttcggg
     3901 cgcttttgtt tcttgccaat aatactcgtt gatagctaaa tgtttaacgg caacttggtt
     3961 cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct tcgctaactc
     4021 tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa acaaggtgta
     4081 ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt tgtcttcttt
     4141 cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac tggtaacaag
     4201 ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg tattcacgaa
     4261 cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg cattagcaat
     4321 ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa cgatttcacc
     4381 ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat tgtcattcca
     4441 aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt tttcccgtgc
     4501 gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca tgggggtggt
     4561 atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg taccatgagg
     4621 aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg caggtaaagt
     4681 ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt ctttatcgac
     4741 ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt aggtattagg
     4801 tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg cgacaggtgt
     4861 tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat tggcatttgg
     4921 ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat tttccacaac
     4981 ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg attgccctaa
     5041 tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt caatggcttg
     5101 atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc gttttattga
     5161 gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa tatgagtatc
     5221 tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat cggacatatc
     5281 tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga ctcctggttc
     5341 aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa cagtgacttc
     5401 tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg ctaaatctag
     5461 ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg taggagcaat
     5521 tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata atttcttttt
     5581 ttgtttttta tacatgaaat gttctccttt caaattaagc acaaaaaaat aagatgctcg
     5641 cttgaacatc tttgtttata aagtcttttt aaaattttta ttgactgaat agaatggatt
     5701 tgagaatgaa tgagcctaaa aatttaatct aaaatacgaa atatactgcc tttaaattag
     5761 aacacatttt agaacacaaa catttcaaat ttaatcaaat ataataaaat atatttacgg
     5821 gaaaaccttt ataaatcaag gtttttcaaa cttattcaat gatacgaaga aaccaggtct
     5881 taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat accaacgatt
     5941 tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa
     6001 tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg ttgagctttt
     6061 tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc caatcgatct
     6121 tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt gtgccggaat
     6181 ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa tatattattt
     6241 gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata ggtaaagata
     6301 agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc ttgttctttt
     6361 ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg ttcggaagat
     6421 atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt ctcttcattt
     6481 cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata aatgtcagac
     6541 caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag ctggaagatt
     6601 aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt tagttcttct
     6661 aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt atttgccata
     6721 ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga gctgtaagac
     6781 ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt ctgacagtga
     6841 acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg caagcggaaa
     6901 aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga aggcaaccat
     6961 tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt atcttcaatc
     7021 tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa cccttttttt
     7081 acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac actatatcgt
     7141 gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa tctgataact
     7201 tggcgagttg gctgattgaa gaaatagaaa atgagaggag aaaaataatg gatgcaggaa
     7261 ttacagtaat gttagataaa caacaaacga atcaattgaa aaattatgtt tttgaaatga
     7321 caaaagaggc tattgatcaa gcaagaattg ataccggatt agaaagacca tttctaaaag
     7381 gaaaagagat ggctaaatat ttgaatgtat catatacaac ttttttgaaa tttaaaagaa
     7441 tgggattgcc agttatttta ttagaaaaaa tggagttgtt ttcaaaagaa gaatgcaaaa
     7501 aatggatatt gagtcatcaa atttaaaaaa cacctcgttt tttaaatttg atgaagtaga
     7561 aaaaattaaa aagagtgttc ccgtcagttt gcagatcggg ggaacactca ataataaagt
     7621 tctgagaaaa ctttatttga atctattata gcaaataaaa tcagaagcag gtgtgacaat
     7681 gaatgaaaaa gaagttgaaa gcttagttga aaagcaaggc tattctaatt ctgatatata
     7741 tgcagaatta tactatcaat atcctatttt tttaaacgta aatgaaaaat ataagaaaat
     7801 gaaactaagt tctaagttag tttatatgtt actaaaagat agaaatagac tttcattgaa
     7861 aaataacttg tatgatgaga atcaaaaaat ttttttgata tttgaaatca aaaatctcgt
     7921 agaaatttcg ggaatgtcaa aaaatacaat cttatcagca ctagatgaac ttcaaaaagt
     7981 taaacttctt gtgaaaagaa aaaatggctt caataaaagt aaaatgcaaa tgttcccaaa
     8041 tttttattat ttgttaaagc ctgttctgtg caaagatgat gtttataaaa ttaataagat
     8101 aacccatcaa gaaaatagac aaaaaaactt gatagttcaa aatttgaact atggaacaaa
     8161 tcaatattct gttgatagtt caaaatttga actatgcgac acatcaatga aaacaagaga
     8221 caaaaacaat tttgctgata gttcaaaaaa tgaactagaa ttatataagt cttataaaga
     8281 tagtaaagat attaaagaat cacataatga tgatcttctt gaatcacttt caactaaaaa
     8341 taataaatta gatgaagaat tgatcgaaac atttattgaa gaaaatcttc ttaaacaaat
     8401 atggggagaa agtattgtga attgcttaaa actggctagc aatagtgatt atcgtcaatt
     8461 cgacaactgg tacaaaaaat tcaaatatgc gattagatct gctgaaaaag aacaaaaaat
     8521 tcaactgaaa attatctatg aaatttgtaa taacaagtat tttgtagatc atgttagaga
     8581 acagctatca atgactttac gtgacttgat acgaagagca aaaacagatc atcgtatcaa
     8641 acaaaaagac gattatatct ttgcttctct aaaaaataaa gcgttagaac taattgaatt
     8701 gcaaatcagc gaaggaatag ataagcaata agaatagaat tgagtcaaat gaaatggagg
     8761 ggtatctcca cttcatttga gaactttgaa tataggaggg gttattttga ttaaaaatcg
     8821 tttaatagat caagtttggt gtctagatca aaatacagat agtctcgaat taatcgagac
     8881 tatttgcttc aacactatag ttctcgattt aagagctgaa aatgataatt gtgtaactca
     8941 tgtcataatt aatcaaaaaa tggctcagaa gctctctgag agcctcagaa aatgggctaa
     9001 gggaggtaac gatgaaaata attgaaaaaa agattgccaa taaaaaaata aattggttgg
     9061 ttgttaattt tcttgttctg atagctatga ttatttttac aagtaatatt gctcatgcga
     9121 gtattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt
     9181 atagacctta cctaaaaaag tacaatggag atttccttag tcagttcggg atttggctcg
     9241 gatgggctgt ggtaaaaggc atgtttactg tgacagattc gattcaaaat atgattccag
     9301 atgtgctgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag
     9361 ccgtaaatac aattgtcatt ggattaatga tcctttcatt gatgtttgtt ggttacaaaa
     9421 tgattactgg aaaaggaaca gtggatttaa aatcggttgg aatgaacgta gtgatgtcta
     9481 tagcattaat acttttaatg ccaaccatga tttctagtgg tattcaattt gctaaaacat
     9541 tttataagga ctctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa
     9601 aacaaggagt aacagattta gcatatatta ataaaacaga tcagtactca agcattgata
     9661 aaacggagga tcgaaataat ttaacacgaa aaaattttca acagacggat ctcacacaag
     9721 tgttaacgga taaagtgatt gataaattgg aaaaagaaaa tccaattgcg gacaatcttc
     9781 gttatgaatt agttgaaaat tctaataatg aatttgttgc tacaaagttt tctgacaact
     9841 tcttatcaac attttctgat agcttgaaat caggctatta ccgataccaa gcaaatctat
     9901 gggggatttc aattgggttg acagcgttag caatcgccta tgtgttcagc gcgtttgtga
     9961 tcattacagc aatactagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcaa
    10021 cagatattga gacaggacag cgttcaaaag ttgttctatc agatattcta caatgttact
    10081 taactgttgg gtttcaagga tttgggttat caatgtttgc tatgtttatc aattttctga
    10141 actcaggaca aggtatctca acaaatattt ttataaaaac aattgcttat atctgtgccg
    10201 tttttgtttt aatcaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac
    10261 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggcgcaatg ctgtttagaa
    10321 aaggatcaaa tggatttaat cgtgctaaag gaagcggaga aggagaagat cgaaaaccag
    10381 aaaagaattt cggcgaaact ctatcaaaaa aagctggcaa aactggaaga gctttgggat
    10441 atgcccatga acgtggatta tccggtttag cttcagatgg agctacaatg gctagtgaaa
    10501 gagcaacaaa accatttaag agtatgcgtg atatagctaa tgataccaag aataagttta
    10561 aagaaggttt agatgatggt acagtgtccg ctattaataa aaatagcaaa cctatgttag
    10621 ctaaaaataa agaagatgaa actggaaaat atgctgattc cgtgccaaca cgtctttcag
    10681 atagaaaaga tggtgctaaa gttgaaaatg ccgatagaat tatgtctagt tctgaacgta
    10741 tgcgtgaagc aatgaaaaat aatgctgaat cgaataataa tcctgcaagc gcaattcagc
    10801 aaaaagtcca acaagatatt gaagaacgta aaaatgctat gcacggtcaa gctaagtctg
    10861 ctgaagaatt aattaatcag aagcgtcatg aagcgaaata cacgcctgaa gcaatgaatc
    10921 gcgaggaaat aatgttaagg aaacgtgtag aagggcggac aggcgctaat attgacggaa
    10981 aagaagcgtt gaccaaagaa agaattcaag aagctaaaaa ttcaaataca ggattagaaa
    11041 aattagtcaa agaaaatcca caaaattcta caagtagtca tggtggccga tcagtagatg
    11101 tccgagaaaa tattcaaggt tcactgaatg gtcatgttgg acggaatgtt gatgtaagag
    11161 agaatttaca aagttcttca aatgctcaag gtactaagac aattgatgtt cgagaaaatc
    11221 tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat gtacaaaatt
    11281 ctacaagtag tcaaggattc aaaacagtag atgttcggga gaacattcaa aaagatagtg
    11341 gagaaatgaa agaaaaaaca caaaaaatca atatcgttga agacagaaag tcagcgaaaa
    11401 aatttgattc aaatcatgaa acagtgatta ttgatagtga aatccgtgaa aatgataagg
    11461 gatcaaaatc aagacgaaga ttcacttatg aagacaatga gttattcaga gatacactta
    11521 atgatcctaa tccacttttc gataatttat taagaaataa aaggggggga ttgtcatgca
    11581 attaattcta aagattagta cgcatgattt cttagaactt gacgaattat gtgaatcagt
    11641 aaagaacgca acaaatgaaa aaatcacggt cactaattat attgaactga tgattcaaga
    11701 agaattaaag atgaaaaatg aaatagagag caatttcaaa tgttgctctc tagaaaaaaa
    11761 tgtgagggga taatcaatgg atgataaaca aaaaatgtac gaaaaaaatc gcaaacatag
    11821 tgaggcctta aattttcgtg tcgataaaaa gacttatctt gaactgacat tgattgcaaa
    11881 acaaagggga atttctagag cagacgtttg ccgtgagcgg ttaaagggac tcaaatatga
    11941 accgccgttg ttagctgaag atgatagggt agttttaacc aaacagttaa atcaaattgg
    12001 taacaatttg aatcaagcgg taaaggcatt aaatatcatt gctaaatatt ttagagaaga
    12061 atctgaagat aaaaaagaaa agagcctaca ttttagtttt gatgctagcc gttacaacat
    12121 ttttaaagat gaacttgcga atagcatagc aaaaaagttg atgattagtt atccagatga
    12181 catttttttg gagtccaatc ggagtgcccc ctcttttttt aaattatcag aagtagaaaa
    12241 agaatatttc gaatattgga tagaaaagca aactagaccg ggtacgaaag aagagtattc
    12301 ccaaaaagtt gatcggaatt taattcactc aattcagatt attgcaagtt taaaagatga
    12361 ggtgaaacaa ctttgggagc tactataaca tcgaaaggac gcaagaaaaa tttatcctcg
    12421 ctattacaat atgcagctaa tccttcaaaa aaagaaaaat gtgttgcagt ctcaggaata
    12481 aattgtagtg atgatagtga ggaagcactg caagaaatga atttagtacg taccaagatg
    12541 aataaatcta aagatatagc acaagcgttt catgttattc attcgtttga taagagtact
    12601 tctaaagagt taagtattga taaaatgcac gaaatagctg tcgaatttgc agagaaagct
    12661 tttccaaatt cgcaaatcat tgttgcaagt cacaatgata aagatcattt tcatagtcat
    12721 cttgtcatta ataatattaa catggaaaca tgtaaaaggt tgcgtattga ccctaaagat
    12781 ttagaactaa tgcgaaaaat taacgatcaa gtcctcaatg aacatggtct tgaatcagtg
    12841 aataatgagt actatgaaaa cttagaaaag agaaccaatc tttattcaaa cgatccttct
    12901 aagatgaaat atggaaaaat taattatcaa gagtttgtgc aaaatgcgat cgaagcaacg
    12961 ttggaaaaat gcgaagtgaa ttcttttgaa gatttttcgg atcatttggc aaaagaatat
    13021 aacattgaag tttatcaatt ttcaaaaaca aataacaaac ttggctatgt tctttacaaa
    13081 caaggtgtaa atttctatga gcgtagaggt gagttaatta ctttaggaag agataaacaa
    13141 tctagaaaag aaaaaagtca gtatgtcgaa cgtactttta gtgcaagaaa gttagggaaa
    13201 agatattcat ttgaatctgt ttctgaacaa cttgaaaaga atttagaaga atcacatata
    13261 aaaaattttt ttaatatgga aagagagcaa gttatgtcgc taattagtgg agtaaattac
    13321 aaaactggtg agttaaatgt accgtttgtg gaattagatt ccatcgcaca aaagcaattt
    13381 agcgaccgtg aaggcgatga aaaattcatt aaatatgatg acttcttgcg agaggtagac
    13441 aagcattttg taaatacaat gtctttagaa gatgatcaag aatacacgat taaacttcat
    13501 acggatgtga atcctcaaac tttacgtagt gcgaaaagta aaacaatttc tattaaaaag
    13561 actgatcaga aagagttatt tctaccggca aagggttttg aaaattgttc tcatacacct
    13621 tctgctaaac agattcacta tcaaatcagt cgcataatga atgacgatgt aaaaatgtat
    13681 cgtgctaaaa aggattatga attcacacaa caaatgattg aaaatcagca aaaaaggcaa
    13741 caaaattttg aaatggaatt ctgaatgggc ttgttagtgg actttccagt tttttgccta
    13801 tgaagagttg cttggaaaga agcagttttt aggacatacg ccagctaccc atctacttgc
    13861 cagtcaatcc gccccgctca cgctaggcta ctttctggca agtcagatgg cgggtaaatc
    13921 tctgagggag caagctccct acagactccc tcccaaaaaa agaaaggtgg aagaaaaaaa
    13981 tgaactatcc atattttaaa gtctcggctt cagaagaaac taaagaaata ttcaacaatt
    14041 tttacaacca aaacaaagga atttttggtt caaaggcaaa tatgtttcgt gttatggttt
    14101 caaatttacc agtgttagct agtccatcaa ataataaatt taatgatcct gaaagcatta
    14161 aatttgaaca aaaaatttca gaactagaat caatgataag taatgaagta attgaaaagc
    14221 tggatgacat tgatcaaaag ttatcgtatt ctctaaaaaa caaatacaaa acggaggaga
    14281 aaaaagatgt ttaaagttta cttcccgttt cagtggctcg ttaaaggaat tcttttagtg
    14341 ctactaggat tgattctttt tggaagttat ctaacaatta aagccagaca ttttccagaa
    14401 gaaaagccaa taaaaattgt aacaaaagat ttaaaaatag gtgaaactta tcaggcagat
    14461 ggggttgaac taacatttaa aaatattgaa gtgaccgaaa aaagaaatga atttgcacaa
    14521 catgatcgtg tattacaagt gacctataca cttataaatg atacagaaaa tccgatacgt
    14581 ccacaacaat ggtcgattga aaataaggat ggtgaagaac tttatgagta tgatatgata
    14641 ttggataatc cagagtatat tgaaagtggc aaagaagaaa cagtaaatta ctattatgaa
    14701 ttaccgattg aaaggcagga ctaccaaatc gtagtacgta gagagaatgg ttacatagtt
    14761 gggttgccat ttactttata agttttattt tgttagtagt ttttaaggta atatcagttt
    14821 tgtgattttt gaaaatggaa ctgggagaat ggctgaggaa aataaagttg ttccgtttga
    14881 aacattagtg ataaataagg aagagtttga gcaacttttt cttgattttt ttattcaata
    14941 tgacatgatt tatgatttta gttcgtcact agatttattt gaattagaag cagaaataat
    15001 agaaaacagt cgagagaaga atggtaaaat cattatttca gtagaaaaat tcaatgaatt
    15061 attctcacaa acatttgaaa tagatccaga ctattatcaa ccacaagaag tttttcgaga
    15121 gtttagaaat aagattattg gttttagtca aactacaaaa tgaaagctta ataaagcaag
    15181 tgttgataga attattcctc aacgcttgct ttattttatg ggcacttcat aatagaggta
    15241 tagagaagag aggtattcat tttggatact attactccaa caaacttcaa agaaaatata
    15301 ggtaaagtgt tgcaagaagt agttcattca aattctgaaa tagaaattac tagggattct
    15361 aaaaatggat tgaacgatgg cgtcattatg atttctaaga gaaaatggga gttgttacaa
    15421 gaagaacttt atttacaacg aacgggtaca tgggcttatg tatctgattt a
//
LOCUS       DAFFFQ010000070        15230 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000070
VERSION     DAFFFQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.070"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            278..787
                     /locus_tag="LQB69_002221"
     CDS             278..787
                     /locus_tag="LQB69_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3526365.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="LQB69_002222"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="LQB69_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3526366.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="LQB69_002223"
     CDS             2322..3302
                     /locus_tag="LQB69_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3526367.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="LQB69_002224"
     CDS             3424..4842
                     /locus_tag="LQB69_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL3526368.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="LQB69_002225"
     CDS             4913..6382
                     /locus_tag="LQB69_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526369.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="LQB69_002226"
     CDS             6392..6859
                     /locus_tag="LQB69_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526370.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="LQB69_002227"
     CDS             6895..7767
                     /locus_tag="LQB69_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3526371.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="LQB69_002228"
                     /pseudo
     CDS             7848..10382
                     /locus_tag="LQB69_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            10538..11311
                     /locus_tag="LQB69_002229"
     CDS             10538..11311
                     /locus_tag="LQB69_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526372.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="LQB69_002230"
     CDS             11304..12095
                     /locus_tag="LQB69_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3526373.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="LQB69_002231"
     CDS             12105..12596
                     /locus_tag="LQB69_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3526374.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="LQB69_002232"
     CDS             12614..13024
                     /locus_tag="LQB69_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526375.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="LQB69_002233"
     CDS             13028..14428
                     /locus_tag="LQB69_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL3526376.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="LQB69_002234"
     CDS             14451..14825
                     /locus_tag="LQB69_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL3526377.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5285 a         2135 c         3047 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaat
     8881 gtttcttaaa tcactttctg aaattgattt gattgaactt tttcaaaatc acatttcaat
     8941 tgaagaaagc atgagtcaat taagagatag aatacttaaa gagtgtgcag gttcttctga
     9001 gtataattat ttagaaagtg acgaatttat agcattaaga aactatgttg ttaataaaat
     9061 atcaccaatt attgattcga caggtctatg tttattagat gaattcttgg cggatatcag
     9121 tctatttata atgtttattg atgtaattga agaaacatca agaaattttt tgctgtctaa
     9181 gttcagaaaa tttcgtatga aaaatgataa acaaaaatta ttggcaaatg agatttggag
     9241 tagtttagaa gtcgaagaaa gtaaacaaga acttttgttg aaatggatac tttttcttgt
     9301 aaagaagttc tatgtgaaaa taccagaaac ccattgtctg atagttatgc acggtaaaat
     9361 aacagcttct gcggatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DAFFFQ010000071        15141 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000071
VERSION     DAFFFQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.071"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..344
                     /locus_tag="LQB69_002235"
     CDS             <1..344
                     /locus_tag="LQB69_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526378.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="LQB69_002236"
     CDS             425..1045
                     /locus_tag="LQB69_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526379.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="LQB69_002237"
     CDS             1746..2030
                     /locus_tag="LQB69_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526380.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="LQB69_002238"
     CDS             2033..2950
                     /locus_tag="LQB69_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3526381.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="LQB69_002239"
     CDS             2943..3725
                     /locus_tag="LQB69_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526382.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="LQB69_002240"
     CDS             3726..4406
                     /locus_tag="LQB69_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526383.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="LQB69_002241"
     CDS             4399..5295
                     /locus_tag="LQB69_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3526384.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="LQB69_002242"
     CDS             5490..7139
                     /locus_tag="LQB69_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL3526385.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="LQB69_002243"
     CDS             7733..8668
                     /locus_tag="LQB69_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526386.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="LQB69_002244"
     CDS             8661..9431
                     /locus_tag="LQB69_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526387.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            9847..10830
                     /locus_tag="LQB69_002245"
     CDS             9847..10830
                     /locus_tag="LQB69_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL3526388.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            11277..12311
                     /locus_tag="LQB69_002246"
     CDS             11277..12311
                     /locus_tag="LQB69_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3526389.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTRAGS
                     NQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKILV
                     INGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELGI
                     DQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMFA
                     KTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHMLG
                     KVVVSGPFNG"
     gene            complement(12400..12723)
                     /locus_tag="LQB69_002247"
     CDS             complement(12400..12723)
                     /locus_tag="LQB69_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL3526390.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(12710..13417)
                     /locus_tag="LQB69_002248"
     CDS             complement(12710..13417)
                     /locus_tag="LQB69_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL3526391.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            13503..13844
                     /locus_tag="LQB69_002249"
     CDS             13503..13844
                     /locus_tag="LQB69_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HBL3526392.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(14015..14863)
                     /locus_tag="LQB69_002250"
     CDS             complement(14015..14863)
                     /locus_tag="LQB69_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526393.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
BASE COUNT         4854 a         2579 c         3200 g         4508 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtggagag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
     9601 caaacaaaaa tagctcctcc tatttttcgt ttggttggac ttattaccgc agatgcaagc
     9661 ttttgaacac catttattcc ggaaaatgga ctgtgataag atttttgtca cagtcttttt
     9721 tttgctcaat cagaaacatc ttttaagctg ttcactttgc ttcttttttt gtttcctagt
     9781 ataatgacaa tgtttttgag aaagattgcg acttcactgc ttgatagaat ggagagtaac
     9841 aaataaatga ctactaattt ttggaaagag ctgccaaagc ctttttttgt tttagcacca
     9901 atggaagatg taacagatgt tgtatttcgc catgtcgtaa agcatgcagc agcaccggat
     9961 gtcttcttta ctgagtttac aaactcagac agtttctgtc atccagatgg aaaagacagt
    10021 gtacgaggaa gattaacatt tacagaagat gaacagccga tggttgccca tatttgggga
    10081 gacaagccag aattttttag agaaatgagc attgctatgg cagaaatggg cttccaagga
    10141 atcgatatca acatgggctg cccagtccct aatgtagcag aacgtggaaa aggcagtggc
    10201 ttgatccttc gtccagaagt agcggcacaa ttgattgaag cagctaaagc tggcggtttg
    10261 cctgtcagcg tcaagacgcg tatcggctat acggaaatgt ctgagatgga agaatggatc
    10321 agtcacctac ttcatcaaga tatagccaat ctttctgtcc atttgcgtac gcgtaaagag
    10381 atgagtaaag tagatgcaca ttgggaagtc attcctaaaa tcattgcttt acgtgaccaa
    10441 atcgctccac aaacactaat cacgatcaat ggagatatcc ccgatcgcca gacaggagaa
    10501 gaattggccg agaaatatgg cgttgatggc atcatgattg gccgagggat tttcaagaat
    10561 ccttatgcat ttgaaaaaga cccgagggaa catacagaaa aagaactgat cggactactt
    10621 cgtctgcaac tagatctgca agatcattat tcagaaatca tcccgcgttc catcacagga
    10681 ctacatcgtt tctttaaaat ctatatcaaa ggatttccag gagcaaatga cttgcgagtt
    10741 cagttgatga atacgaaatc tactgatgaa gtacgagaga tcttagagac ctttgaaaaa
    10801 gaacatccaa cgcttttcag tgaacaatga tttcttgctt gaacaccgtg aattagaggt
    10861 cgtgaaaaag ctgtcctgta tcaagtaata agaacagcca aacaaaaata gctcttcata
    10921 ttttgtcgtt ttttgagcta ataccgaggg tcagttgatt gaacaccgtt tatatcagag
    10981 gttgtgagcc tgccgtttaa ctctgagtca caaggagcaa tttcaaaaaa cagcttctca
    11041 tgtttgatga atattttgac ttgtgaccga agagttggta ggagaacacc gtttataaga
    11101 ggttgtgaca aaagtcttgt cgcaacctct tttttaacca aaaaagtgca aatagtttac
    11161 tttaagtaag agaataatta tgatgaataa aaaaggtgga acaaatagca tactatatat
    11221 attgaaatga acactgtatg gatgctccaa cttgatgaag ctaaggagga attcgtatgt
    11281 cagaaaagga attaatgaag gtagtcggtt tttatgaagg gttgccgatc gatgatccaa
    11341 aaagctttat tgatgaaaaa gaacagatac ctgtacccag ttcacgggat cttctggtaa
    11401 aagtcaacgc agtatctgtt aatcctgtcg atacgaaact tcgccaagat aacgggataa
    11461 gaaatgcttt acgtatctta ggttttgatg gagtcgggaa agttgttgct gtaggtgacg
    11521 aagtgcaaaa attttctgtc ggcgatcgcg ttttttacgc aggtacaaca agagcaggaa
    11581 gcaatcaaga ataccaacta gttgacgaac gtattgtcgc tcttgcacca aaaaatcttt
    11641 cagatgaaga agctgcagcc ttgccgttga cctcattgac cgcttatgaa ttgttatttg
    11701 aaaaatttgg tctaactcca gaagaaaatg ccaatcgcgg gaaaaaaatc ttagtgatca
    11761 acggatccgg gggcgtcggt tcgattttga atcaactagc gcattgggct ggcctagaag
    11821 tctacgcgac tgcaagtccg aagaattttg aatggctgaa aaaaacaggt gtagactatc
    11881 caatcgatta tcatcacgac ttaagtaaaa gttttcgtga attaggaatc gatcaagtag
    11941 agtatgtcgc tgtattattc gatattaccc gctactttga tcaaatcaag caccttgtcc
    12001 gaccatttgg acatgtggga acgatcgtag ggatcaaaga gccgttagat ataggtgttt
    12061 ggaaaaacca atctatcagc ttcgactggg agtatatgtt cgctaaaaca gactataact
    12121 atgaaatcga aacgcaagga gaagctttag cgcacattgc tgctttagca aatgaaggga
    12181 ttatccgcag tacattgagc aaggtctatt cagatgggat gaatgcgaag aacttgaaac
    12241 aagcaaccaa agacgtagaa acaggacata tgttaggaaa agtcgttgtt agtggtccgt
    12301 tcaacggata agcaagaata cgccgatttt tttcttattt gactttgata aaaacaagag
    12361 cgcatgacag taatttgcca taagctcttg tttttttatt tataataagc gaatcaacga
    12421 aagggaaaga attcctgcca agacgatcac caataagctt tttgaaagaa tagcagctaa
    12481 aagtgtagga actgaagcca aagcattttc gagattgatc tgcggcagat gtccgatatt
    12541 ttgggtaaat agactgctga accataaagc agacataatg acgatcggga caaaacttaa
    12601 atactcaagc aacggttgcg gtaaatcgaa ttttttcagt aagacaaatg gtaggaccct
    12661 agaaagccaa gttgctaaag tacaacctaa aatagtcagc aatgtaaact caaaagaagg
    12721 catgtttgat ccacaccccc aatccgcacc caatcagtgt tacaactaca atcactaagt
    12781 tgcttgggat aaaaatcagt ccgacatata ccagaatcaa agtgagacca atcaggatca
    12841 actgtaatcg tttagaggtg tttctatcag aaatgacttg aagataaagt aacccgataa
    12901 acatagcgac gatggcgaaa tccaaaccaa actttttcgg atcagtaata aaattcccta
    12961 aaagtgcgcc aacaagagaa gaacccaccc acgtcaaata agaaatccaa ttagctgtat
    13021 tcatccaacg aaagtttaac ttttgtccag tatagttcaa tttattcatt cccagtgcga
    13081 aactttcatc tgtcagcaat gtgccaatca gcagattttg aagcagccga tttttcttga
    13141 aataaggagc aatcgtcata ctcatcagaa tcattcggga attgactaga aaggtcgaga
    13201 aaacgatgga aagcaatggg ctgtgactag caagcatgct gactgtgata aattgggcag
    13261 aaccagcata gaccagcagg gacatcaacg tgacgactag tggatgaaat ccggcagctt
    13321 tcccaacgat accaaatgca agtccaatac cgatataacc gaaaacggta ggtaacgtgt
    13381 ctttgatcgc agtttttata tctaactttt catccaataa caaaactcct ttagcacgac
    13441 ataaattttc ataattgtcg ttaaacttgt gaatattcta aatttttaat gagcatttgt
    13501 caatggtggg attgaaacgt aaagaactag cgggtcgcag atttacacgt attgtttcgg
    13561 tgggaggagg cgcgaaaaat cttgcatggc tacaaatcca agctgatatt ttcgatgcaa
    13621 cggtctattg tttaagtgtg gaggaaggac ctgcattagg cgcaacaatg attgctgcac
    13681 tagggttagg gtggttcgac acacctgaaa gttgttgtca gaattttatc caatatacag
    13741 atgcagtgga acctctctta caaaatgtag aaaagtatca taaaatctat caacaatgcc
    13801 ggaaaatcta tccagcaacg aaggaaattt gtcatgactt ataaaacaaa acgcgtacct
    13861 aatagtcaaa tgactaatat tgggtacgcg ttaagtcttc tttacttcat cctttgcatg
    13921 attcgtgttt ttaaagcaag gtctgctgta tttcttgtgg atttaggatg gttgttatcg
    13981 tttacttgct atttttcatt gaaatcgtct ttctttaccg tttctttagt tttttatcta
    14041 tttttttctt ttggttagaa aattcttttt cccgaaagtc ttctttcagg atttttatag
    14101 agtagcgttt tgaataagag gcgtcagtat gatcgacttg gcagacgaac caagagaaca
    14161 tttgcctttt ttctgcatga ggtgcttctg ctaataattc tttccataaa taataacagt
    14221 cctctatcag aagaagggtt cgttcatcct caggctccca atagaatgtt tcgagagtag
    14281 caatcacttc attgatcaat tcgaatgcgg gaagataggc attttgctga ataagcgatt
    14341 caactgcttc ttggatgaac tttcgcatct ccatgatgaa cttcgctgtt ttcctgtttt
    14401 tgtttttctt gataatatga tcgtatttat ggatgatagt tgttaattta tctgaataat
    14461 ttattgcatg aaatggcatc ttaactttga agcgttcgat aaagtgcgga tgttctataa
    14521 gaatctctgt taaaagctcc cgtacaagag aggaactagc ggaggaaaca agggacgaga
    14581 gttcggaaag tgagtagttg gattttttta ttggacgttc ttcgtaagcg aataagacag
    14641 cagccatgtg tttgcatttt tttccttcta tggcatatag acaatcacaa gtcatgttgg
    14701 tcttttcatc ccaagtgatc actaccttat aatcttccgt gccagaaact gttgcggaca
    14761 agtacttgct tgtatagttc aaatgacgga caaaaccgtg cagatgatag ctgtaaccgc
    14821 gttccaaaac gttttcagga aaatactcaa gccaattatt catctagttc tcctttcatg
    14881 aaacacttat ttatatttag tataaagaaa cgtttaagaa aaaagtagct gattttacat
    14941 atttgataat cacttttgct cgaattgtca aaaattcatt ctccttttct tttcttagag
    15001 aatgtgagaa aaagtaaaag aatagcgtgt tttaaagatg gaactttttt tgttttttat
    15061 ttatctgaca aaaatgaagt gattttgtta gaaataaata attgttccaa ttgataaaag
    15121 aggtatgatg tatcgcaaag a
//
LOCUS       DAFFFQ010000072        14837 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000072
VERSION     DAFFFQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.072"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            417..1031
                     /locus_tag="LQB69_002251"
     CDS             417..1031
                     /locus_tag="LQB69_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3526394.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1804..3543
                     /gene="yfcC"
                     /locus_tag="LQB69_002252"
     CDS             1804..3543
                     /gene="yfcC"
                     /locus_tag="LQB69_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL3526395.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3700..4884
                     /gene="iadA"
                     /locus_tag="LQB69_002253"
     CDS             3700..4884
                     /gene="iadA"
                     /locus_tag="LQB69_002253"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL3526396.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            4995..5198
                     /locus_tag="LQB69_002254"
     CDS             4995..5198
                     /locus_tag="LQB69_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL3526397.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5299..5961)
                     /locus_tag="LQB69_002255"
     CDS             complement(5299..5961)
                     /locus_tag="LQB69_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526398.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5974..7476)
                     /locus_tag="LQB69_002256"
     CDS             complement(5974..7476)
                     /locus_tag="LQB69_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526399.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8024..9703
                     /locus_tag="LQB69_002257"
     CDS             8024..9703
                     /locus_tag="LQB69_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL3526400.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9830..10309
                     /locus_tag="LQB69_002258"
     CDS             9830..10309
                     /locus_tag="LQB69_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL3526401.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10528..11208
                     /locus_tag="LQB69_002259"
     CDS             10528..11208
                     /locus_tag="LQB69_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL3526402.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11216..12547
                     /locus_tag="LQB69_002260"
     CDS             11216..12547
                     /locus_tag="LQB69_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL3526403.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12562..13524
                     /locus_tag="LQB69_002261"
     CDS             12562..13524
                     /locus_tag="LQB69_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL3526404.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13635..13973
                     /locus_tag="LQB69_002262"
     CDS             13635..13973
                     /locus_tag="LQB69_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL3526405.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14052..14759
                     /locus_tag="LQB69_002263"
     CDS             14052..14759
                     /locus_tag="LQB69_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL3526406.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4761 a         2578 c         3192 g         4306 t
ORIGIN      
        1 ttttttgtat tttcttgctt gcacttggat aagagattac ttacaataaa ttcagataga
       61 aacagctgaa taaaaaaagg ctccctgacc cttagttggc gctaagaatc agggaatgac
      121 cagtcacaca catacaccct gaccgatcat tttgccatag tcaatgcagt gcatcgacct
      181 aatttattgt acctaatcta gggtgaaaat tctaccccta tgtagatatt ttccatggtc
      241 tgcacgttgt tttatttgct atgtcaaggt attgggcgta ttcgttcaat gccttttttg
      301 tgtttgaaac agcaaagacc acatgtgtta ctgtctaact actgtttttt tatgtacttc
      361 cttagattag gcagctgttt ctattcatgt caagtatagc taggcgggag agataaatga
      421 gtttatgtta ttttgactat tatcaccaat tggaagagaa attaaggata gaagagccgc
      481 tgatgcttca agctggcact tggatcgcga aacaaattaa tcaaggtggt agattgcaga
      541 tatatgctag ccgcacacta agtggtgtag catttgaatt ttgggatcaa accccggatc
      601 ttgtaccaag tatattgatt gaaaatccag ccggaggtgt ctatgaaacg ctagaaggtg
      661 caggacaggc aattattgat cagctttctg tcaaatcaga agacattttt cttcttcttt
      721 cgaatgaagg aagaaacccg gcaatcatcg aactagctca gtggatcaaa gaaaatggtc
      781 atccattaat cattgttact ggatttgatt tgtcacgaag tatcaaatcg caacatagca
      841 gtggtttgcg attatttgaa tttgctgatt tggttttgga caaccacgcc aatttacatg
      901 atgcagtctt gactttgcca aatctagatc cagcgatctg cggaacagcc tctcttgcaa
      961 cgatcttttt gctgcagcaa gttttgtatt ttacagcaaa acagctagtc caggacacaa
     1021 gtggaaaata gttatccaca tgttgacaaa gttccttaaa aaaataatgt tccacataaa
     1081 aatggagcgt ttgaaaaaaa gaaaacgatt attaatgagg attaacaacg actacttttt
     1141 ttgttttagt tattttaaga tggatcatct ttgattttaa gcttgttttg tttaaagttt
     1201 gttattttta taagttgttt cacagttatg agaaatgagt tataagaata tgtagtgtca
     1261 gaaaaataaa aagtagtgga aaaagagaca agaaagaatc catatggatt ctttcttgtc
     1321 tcttaataca cgttgatgaa agagtcttcc ccaaaactct ttacaaagta attatagtac
     1381 atttgttatc attattgcaa tgaagtttac ctgatataac aaaattgtcc aacaggatac
     1441 ctttttctaa tagaaataaa atacacaaaa attgaaggat agataatgga tatttataag
     1501 tgtttattat tttttttgca atgaaattca atttttcaat gattgtaaaa tgaaaaaaac
     1561 aggacgagat tttctctaat ctaaaaataa aaaaagaatt ttaaggaaaa acagagaaat
     1621 aaaatactta atataaatgg aaagagaaaa aaataatcat acataggaga aaaaaaacaa
     1681 aatagaaaaa agtgattttc taataaatta ttcatgtgga aatgtcattt ttactgtata
     1741 attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata
     1801 aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca
     1861 ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc
     1921 gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca
     1981 agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg
     2041 tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat
     2101 gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa
     2161 aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc
     2221 tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc
     2281 ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct
     2341 gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt
     2401 accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt
     2461 ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca
     2521 gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac
     2581 tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg
     2641 atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt
     2701 gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg
     2761 atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat
     2821 tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat
     2881 aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa
     2941 aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga
     3001 atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc
     3061 caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt
     3121 cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat
     3181 gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc
     3241 ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg
     3301 ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct
     3361 tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt
     3421 gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa
     3481 ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag
     3541 tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac
     3601 cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga
     3661 tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt
     3721 gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt
     3781 ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca
     3841 ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga
     3901 ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca
     3961 gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact
     4021 gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa
     4081 ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca
     4141 attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca
     4201 agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga
     4261 aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca
     4321 cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc
     4381 acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc
     4441 actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa
     4501 gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat
     4561 ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga
     4621 agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg
     4681 gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa
     4741 ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat
     4801 attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg
     4861 gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg
     4921 ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa
     4981 gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag
     5041 tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag
     5101 ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag
     5161 tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa
     5221 tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg attttttatt
     5281 tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt ttagttcaaa
     5341 gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa tacagcgatt
     5401 ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg tttcatctag
     5461 atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag cacgaacgat
     5521 agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg ctaatatttc
     5581 atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct cttgcttggt
     5641 cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag gaagcaagtt
     5701 gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc cgattttgtt
     5761 cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc cagaaataag
     5821 cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca tttttccagc
     5881 ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat accaaaatcc
     5941 caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt ttcttgggtt
     6001 taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc caaatgctac
     6061 tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc tgacttgatc
     6121 agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac cgccttgttc
     6181 ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg tcgtggtttc
     6241 tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc cgctaagcga
     6301 agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt ggagaatgat
     6361 tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac gctcccgaat
     6421 ggtaatcata atgatcagag taagtatgat aataccggct acagccacta gtaaaacaac
     6481 atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat acatggaatc
     6541 atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt tgagaaactc
     6601 atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt tatcgtcaga
     6661 tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg acggattcat
     6721 gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa ttcctttgat
     6781 cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag tatctccgac
     6841 tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg tgcctttgtc
     6901 gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa aattactatt
     6961 ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt gcggattccc
     7021 gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt cttcacttga
     7081 atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg aactttcaaa
     7141 ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta aggaaactgg
     7201 tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc ttgaagaagt
     7261 ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg ttgctccaac
     7321 ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga ggccagctag
     7381 cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc cccattttac
     7441 ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc ctagtagatg
     7501 catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt ttgcacacca
     7561 tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa atgtggtaaa
     7621 atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag caaaagaaat
     7681 cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc tgatactagc
     7741 gatttagttt atttgttgta taataatggg gaacagctga atacttatat aagtattctg
     7801 gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa taaaatgccc
     7861 atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc ataagttgag
     7921 aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata aagaagaaaa
     7981 tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta ccattaaaat
     8041 tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag aagtgggcga
     8101 ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt tggggatcga
     8161 cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag ctggtgtctt
     8221 tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt caaaaatttc
     8281 tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg tcagtcgtaa
     8341 tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag aaatagactt
     8401 tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt cagtggggat
     8461 caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat ttgaccaaac
     8521 ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca aagaaggagt
     8581 cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag cttctgaagc
     8641 ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta tcatcgtcgc
     8701 ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag aaaaagcaca
     8761 acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag cgatgcgttt
     8821 agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca tgaaaaacta
     8881 tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca tcaagtctct
     8941 gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag atttggttta
     9001 tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg aagatatcat
     9061 ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt ctggccatgc
     9121 caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt ttgtgccagt
     9181 tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag tagggatgcc
     9241 atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga acaagcgtat
     9301 gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag gcataggtga
     9361 tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga ttttcgtagc
     9421 ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa ttacttcacg
     9481 gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca atatgattga
     9541 aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat taaagcaaga
     9601 catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc cagtgatctt
     9661 gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa gagtaagatg
     9721 aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata atttttgaat
     9781 acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa tgatgagtca
     9841 aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac ttgattttag
     9901 tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg atccagcttt
     9961 gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg cattgattcc
    10021 tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc gtaaagttgg
    10081 ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag caaacacccc
    10141 tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt ctttaaaaga
    10201 aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga taaaagtagc
    10261 cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag ggacgagaag
    10321 agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt acttggtgca
    10381 tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca atgaaaatat
    10441 atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt aataaaaaag
    10501 atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca ttcctgttta
    10561 acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac aggaaatcca
    10621 ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac tcatggtcat
    10681 agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac aattatcagt
    10741 attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg tatgaatatc
    10801 ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca gcatagttca
    10861 agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat tattattcaa
    10921 gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga tctaggattg
    10981 cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa ctatacgatg
    11041 gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt agttccaatc
    11101 cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga gttactggaa
    11161 gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc agaaaatggc
    11221 gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca atcgaatttc
    11281 tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc gtgcaatcaa
    11341 agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca tccgtatcga
    11401 acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca ggatcattga
    11461 aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat tcgtcatcgg
    11521 cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa tgatcgtcgg
    11581 caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat tgatcacagg
    11641 aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa tgccgatctt
    11701 acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt taagcgacca
    11761 attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg aaaagactca
    11821 ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag aaacgcagca
    11881 ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta cggcaaaaga
    11941 tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag aaccaatcag
    12001 tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg atgggctaga
    12061 agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc ggcaagatgt
    12121 aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga tttcggatca
    12181 gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt tggaaaaccc
    12241 taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca tttcttttgg
    12301 cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga accaaaaacg
    12361 aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat tggaaagcga
    12421 attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac tggatatcga
    12481 agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag taatggaacg
    12541 ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac aataaaaaaa
    12601 tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt aggatcacaa
    12661 gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg tcaagtaggt
    12721 gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga tgtcttctac
    12781 caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag tgatcaaaga
    12841 tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga cccttatgga
    12901 gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc agaatttgct
    12961 ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact ttatgcagga
    13021 atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac attagagatt
    13081 gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga gattgaacaa
    13141 atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca ggtggatgaa
    13201 aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg aatcgatgat
    13261 tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct ggcttggggg
    13321 atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa ggggccgatc
    13381 atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag tggtgcgaat
    13441 gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat atgccaatcg
    13501 tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg tagtaaaaat
    13561 cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac taaagatttt
    13621 aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag tacacatatg
    13681 aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa gaagaacaag
    13741 aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt ctgttagagg
    13801 gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt gccattaaac
    13861 aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc gattgcaagg
    13921 tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat taaagaatat
    13981 tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa acgaaaagga
    14041 gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca tttcttttgg
    14101 tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt actttccgaa
    14161 aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca caatatatat
    14221 tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat ctcacagata
    14281 actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag tctacttcag
    14341 ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat gcttcgggtt
    14401 atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt tcgatgattt
    14461 cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta atcaatatcg
    14521 ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca gtcgaattta
    14581 acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg gaagaagaag
    14641 taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca gcagcgcttt
    14701 cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga agagattaaa
    14761 ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt agctgattga
    14821 ataccattta tttggaa
//
LOCUS       DAFFFQ010000073        14631 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000073
VERSION     DAFFFQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.073"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LQB69_002264"
     CDS             complement(<1..1416)
                     /gene="nhaC"
                     /locus_tag="LQB69_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL3526407.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1819..3042)
                     /locus_tag="LQB69_002265"
     CDS             complement(1819..3042)
                     /locus_tag="LQB69_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL3526408.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3073..3912)
                     /locus_tag="LQB69_002266"
     CDS             complement(3073..3912)
                     /locus_tag="LQB69_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL3526409.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(3929..4609)
                     /locus_tag="LQB69_002267"
     CDS             complement(3929..4609)
                     /locus_tag="LQB69_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526410.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4611..5684)
                     /locus_tag="LQB69_002268"
     CDS             complement(4611..5684)
                     /locus_tag="LQB69_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3526411.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(5740..5978)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(6021..6254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6331..7002)
                     /locus_tag="LQB69_002269"
     CDS             complement(6331..7002)
                     /locus_tag="LQB69_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3526412.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LQB69_002270"
     CDS             complement(7089..9767)
                     /gene="mgtA"
                     /locus_tag="LQB69_002270"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL3526413.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10426..10863
                     /gene="ndk"
                     /locus_tag="LQB69_002271"
     CDS             10426..10863
                     /gene="ndk"
                     /locus_tag="LQB69_002271"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL3526414.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(10885..11751)
                     /locus_tag="LQB69_002272"
     CDS             complement(10885..11751)
                     /locus_tag="LQB69_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3526415.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(11911..14004)
                     /locus_tag="LQB69_002273"
     CDS             complement(11911..14004)
                     /locus_tag="LQB69_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3526416.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         4246 a         3137 c         2497 g         4751 t
ORIGIN      
        1 ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt tttttgagat
       61 tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac tgaaaaatac
      121 gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac ttccggcaat
      181 tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc tacctaatac
      241 agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag gcagaataac
      301 agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca atgttgtaaa
      361 aatcaaattt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg ttgagatcaa
      421 gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg acacagtagg
      481 catcatactt tcgattcctc cgcggctaag cagtgaatcg atttgttggt ttccagtatt
      541 tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt gtgggttttg
      601 aataaaaatc attcctactg ctacagcgat attcaacaaa atcgttaaaa ttgccggcac
      661 ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga ttgaaatagt
      721 aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg cggctttact
      781 tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca aatgccggga cagttgacca
      841 catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg ctagatttgt
      901 tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa tagctccaac
      961 aaccagcgct ggatttaagc ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt
     1021 tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca caacaaatac
     1081 agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta gagttggaat
     1141 gatccctgct tgtatccata cagcaatcaa tgaaccgact aaaataaaaa taaagattgg
     1201 gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg tcgggtatct
     1261 ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga tattaggtga
     1321 gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta atacaagtgt
     1381 tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt ttttagttgt
     1441 tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat ccgcgattcc
     1501 tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc gcggtaccac
     1561 cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt atggactttc
     1621 catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt ctgaacaaaa
     1681 cacctaaccg ctactgcgtg aaaagttgac ttgtacgaag tttaccttcc ataagatcat
     1741 ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta taactttcac
     1801 acacaagttt attttttact agaaagtagc gccctcttgt ttttttcttg ctactatttc
     1861 ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat caagagctgt
     1921 attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac ttttagcagc
     1981 agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta ccgtaggctg
     2041 gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac ttgttctttg
     2101 ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga agaataagtc
     2161 atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc caaactcaaa
     2221 agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt catatacttt
     2281 atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat
     2341 gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat cttttggcaa
     2401 ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca aagcatttac
     2461 tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga tgtcgactgg
     2521 atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga cgcataacgc
     2581 tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg atccgttctc
     2641 gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt catcaatgaa
     2701 ttgcttcact atgctggcgg ctaatacgga gtaagtacca aacaaattat gcccgcatgc
     2761 atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg gaccgggttt
     2821 attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt tttctaccca
     2881 aaacctttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact catagttact
     2941 tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta aacgatattg
     3001 gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc
     3061 tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg atccttttga
     3121 tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta ttttttgata
     3181 cgttttattg ttttcatctt cttttcttgc aacgatgata ttcacataag gacgtgcatg
     3241 gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag catctacagc
     3301 tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg tttgggaagc
     3361 atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa cagtgggcgc
     3421 ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca gaagagctct
     3481 gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga tatcagctac
     3541 atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc cgatcgatac
     3601 taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt
     3661 caaatcaatc gatccatctg ctaaagccac atttggttga ttgtaatctg taaatttcac
     3721 gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc atacatctgt
     3781 atcttctcca ataatcccta gttttacgat agtttctttt ttgtcagttg tatctgcttt
     3841 tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta caaaacgaat
     3901 aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc agttgattac
     3961 tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca atcgttacat
     4021 ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg cctgcaccta
     4081 ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc gagacccgaa
     4141 tgataccagg caaaccttct aacaaataca cacgtaaaat gataccgatt ggactggttc
     4201 ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg ttctcgattt
     4261 gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct ttcgcaccaa
     4321 tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag ataatgaaag
     4381 ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga cggttagcta
     4441 gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattcca atcatcccgc
     4501 caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg acaaattcat
     4561 ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct
     4621 ccttcgatca ctttgatacg actatctaaa tcgttttttt gtaaaatatt tatttttaca
     4681 ccagatttga tcaaataatc caatgctttt ttccgctgtt tggtttctcc agctaaagta
     4741 acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata taaaatattc
     4801 gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt ctgatttcca
     4861 atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc agaatgggca
     4921 atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc tttggtcaat
     4981 tcttcttcag gattgctgaa gatctggatg ctgctacctt tttcaacgat tcttccatct
     5041 tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg agtgatcagg
     5101 acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat ctgaagggtc
     5161 gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg ttcattcgcc
     5221 aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt aggataagca
     5281 gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg acgacgttcc
     5341 tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt
     5401 tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt tcgcaattct
     5461 ttgggagaga gctgactcag attcgtctga ttgacaatga cttctcctgc tgttggtttc
     5521 tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta accgacgatt
     5581 ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg tatctctttt
     5641 tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat tcctccttat
     5701 ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt
     5761 gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta cgatcatact
     5821 ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga tgaattgctc
     5881 gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa
     5941 aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt
     6001 actaacataa actatgtcag taaaaggacg acgtatcgcg gtaccacctt ttttcggaag
     6061 tttccttccc tcaagcccaa catctcaaaa agagagattg gcgttcgttt taacggtcga
     6121 acagttccga atttgcctac ttatcgacaa attattctcc aaggccattt tcagcttttc
     6181 ttacttgact cttctcacca gccagagcct ctctgcaaag tttccaaact tactttcctt
     6241 ttcaacgaaa tcgatatttt gttgttggga ttaatctaca tgaggaaaac tgaaaagtca
     6301 agtacgaaat tcaaattatt ttttctatat ttatttttct gtttttttat ttaacatttc
     6361 aataacagtt cgaagatctt ttccttccgc aagaagtttc aatttttcct tttcttcgac
     6421 ggtataattt cggacaaaac tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga
     6481 agcagctgtt gctcctacta aagaatcttc cactgcaaag gctgaactag cttctaatcc
     6541 tgttcgttcc aaagctgtca gataaggaat cggtgaaggc ttgttctccg acacttcgtc
     6601 tccgaatgta aacgtgtcca agtaatcagc taatttaaag tgatccaaga tatctgttgc
     6661 tcttttcgta acagtggtcg tagcaatgcc tagtttcata tgacgttctt ttaagtctgc
     6721 caatatttct tttgcatatg gttttaagtg gatggctccg cttgtcagct gttgataaaa
     6781 atattcttca cgcttcgctc ggacttcctg tactttttca gggctacctg ctgctttcac
     6841 taaaatatca aaactttgtt tccagttttt tccgccccaa gcgtcaacga tttcttttgg
     6901 gatatggata tcaaattgct gaaatcccca aagccagcca tcacgatacg ttttttctgt
     6961 gtcaactaac aaaccatcta aatcaaatat tgcacctttc atctatacat taatcctttc
     7021 caaaacaata cacatagttt agcacgactg gtccaaaaag aacagaagca aagaaccagc
     7081 cttctcgttt ataagagttc ttggaatcga cggatataga ttttcttcac agctgtcaca
     7141 agcgccagat aagcgatgac tgtagcaaat aaccataacc agtaattgcc aggaagtggc
     7201 atcaagccta attcagcacc aaaacttgta aaaggaagga cagaaccgac aataattcct
     7261 aatgtagtga taacaaacat ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt
     7321 gtacgcagtg catggagcac taaggtttgt gtccatagtg attcgacaaa ccatcctgca
     7381 tggaataatg cgatgaaaat cactcgctgt gaactatcca atgtatggaa acttccgccc
     7441 actacagcag gacaaataac aaaatacatc aacaagtaag tagtaatatc aaatatcgaa
     7501 cttgttggac caaagtaaac catgaattcc ccaatactag atgcattcca tttttttggc
     7561 tcgtccaaat attctttatc catccgatcc catggcaagg aaatgcagga aacatcatag
     7621 atcagattca aaaacaaaag ttgtaaaggc agcattggta aaaatggcag gaacgtactt
     7681 gcaatcagca cggaaaacat attgccaaaa ttagaacttg ctgtagcctt gatatatttc
     7741 atgatattcc caaaagtttc tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct
     7801 ttttctaaaa ggatcacgtc tgcagattct ttcgcaatat ccacagctgt atctacagaa
     7861 ataccgatat cagccacttt cattgcagga gcgtcattga taccatctcc taaaaatccg
     7921 acagtatgac catttttacg taaagctgtc gtcaaacgtg ctttttgctg aggattcaat
     7981 tttacgaata cttcatggtc ttcagctact tgtgctaatt catgatcact catttccgag
     8041 attttctcac cggtaaccaa ttcattgatc tctagaccta cttgacggca cactgaacgt
     8101 gtaactaatt cgttgtcccc agtcagaact tttacagcta caccatgttc tttcagagct
     8161 ttcaacgctt gtttcgttgt ttcttttggt ggatcaagga aagcaagata accaatcaaa
     8221 accatgtctg actcatcttt gactgaaaat tctccaacga cgcttggatt ggtcttttgt
     8281 gcgacaccaa tcactcgtaa gccatcttca ttaagtgact gtacttttgc aagtacggct
     8341 tcttttcgtt cttttgttaa tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg
     8401 ttcagcattt cttcaatcgc gccttttgtg atcatttgtg ttttaccagc tggatcagca
     8461 accacaacac tcatacgacg acgttcaaaa tcaaaaggaa tttcatccac tttttgataa
     8521 ttgatcttat ctgtatctaa agtttctgtt gcttcatcaa tgatagccac atctaacaaa
     8581 tttttcagcc ctgtttgata gtagctattt agatacgcat ggcgcaaaac acgatcatct
     8641 tcatttcctt cacaatccat atgatattca agaatgattt tatcttgtgt caatgtcccc
     8701 gttttatccg tacataacac atcgattgca ccaaagttct ggatggcatt caaatttttg
     8761 atcactgttc cttttttggc catcacactt gctcctttga ccaaattcgt cgtaacgatc
     8821 attggaagca tttcaggtgt tagaccaact gctacagata gtccaaaaag aaaagcttct
     8881 agccaatccc cttttgtaag cccattgatg acgataactg ctggagccat caatgacata
     8941 aatttgatca gtagcatgga tgttttatgg atgcctaatt caaagcttga ttgaattggt
     9001 ttttcagata gcgtttctgc tacatgacca aatagtgttt catttccaac agaaacaatg
     9061 attccttccg cacttccact gatgacatta cttcccataa agacaagatt ttcgtaactt
     9121 gtttcgcttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg atagctttcc
     9181 cctgtcaatg ctgcttggga aatgaataaa tcctttgcgg acaataaacg catatccgct
     9241 ggtatcatat cccctgctga aagccgaaca agatctccac agacgatttc ttccatagga
     9301 atctctgtgt actttccatt acgttttaca gctgccgtta ctttcacaag tgatttcagt
     9361 ttttcagccg cttgattgga acgtacggac tgaatcaacg tcattgtccc gctgacgata
     9421 accatagtga agataatggc tacaccgaaa agatctttat ctccaggagc tgctaataca
     9481 tattccgtga tgaatgaaat aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg
     9541 tatgctttca atatttctac aatgaatggt gtttttttgc catatgaaat cgcattttca
     9601 ccgtaatttt ctcttgaacg ttcgatttgt tcttctgtca atccatttgc tgatgcatgg
     9661 aatagttcaa ataattcttg tttcgttttt ccagcaaaag ctgcatagat tttttgtgta
     9721 tctttctttt tttccatctc aatctctcct ttgtgtccaa aagacagctg ttctcgctgc
     9781 ttttggtctt tagctattca tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag
     9841 atagataata gccctggcct ttcctctaga tccttgtttt tcgaatgact gtcatccatt
     9901 tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt tttcgaatag
     9961 ttatttttcc aattagacca cctccttttt atttttcacc gatggggatt tgcggcaaca
    10021 aaaaaacctc tcgccaaata ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc
    10081 tcagatctca tcgttgagtt ttgacactgt acaacgtaaa aagcagatac ctgcttttag
    10141 ctaccttatg aaaagcctta tctcgacaat tcctgttcta cccattggcg tctctcgacg
    10201 tttttgggca gtagcctatg tttgtatagg agcctcacct aacaaatctt attcggtttt
    10261 ccttcgttaa tataacaact aaaaaagagg atgtcaaatc aattttccat gtttttcata
    10321 ctttttttaa aatacacacg ttatattcct gtctcacgcg gttttatttg tctcattttc
    10381 cacctcccgc tataatagaa atatagtagg aaacgaggtg ctgttatgga aagaacactt
    10441 gttattatca agccagacgg cgtgagacgc catttagtcg gaagcatcat ccaacgtttt
    10501 gaagcaaagg gactggccat tgctgaaatg aagtttgaaa ctatgactcc tgaattagca
    10561 aaagaacatt atcagcactt gactgagcgc agtttttttg atgaactgat cgattacatg
    10621 acttccggtc ctgtcgttta tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt
    10681 aagatggttg gagcaacaaa agctgccgat gctgttccag gtacgatccg aggagactac
    10741 gcgctgccgg ggactgaaaa catcattcac gcttctgatt cccgtgatgc tgcggtaaaa
    10801 gagatcgccc gctttttccc gccattcgat ataaacaaac cgtctgaaaa agcggcgaag
    10861 tgatacaaat tctaaactgc tggtctagtt gctgggttca gcaatggcca gcagtttttt
    10921 ctctaaggac ctccgttgat tctttcctaa catatattcg agcactgtga agaattcttg
    10981 gtagtctttc gtcttttctt cccctaaagc agcagaaatc aatctttgat aataatctgc
    11041 catcaatgtt tcatagatca ctgtacttct ttctttatgt gctaagctcc gtttcatatc
    11101 ttcattcaca ctacgtgcct ttttcaaccg accattttct atataaaacg aagtcaggtt
    11161 cattaaaaaa gtaaagagat actgctcatg attgttcaac tgtgctaatc gtcgcattct
    11221 ttttttgatt ttcaaataat tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc
    11281 agctagaata gatagttcaa attgaccata gtctggtatc tgcatcaagt attcttcaat
    11341 gattcggcat tcataacgta aaaattcaaa ttctgtctca gatacttctg ttttttcgac
    11401 catgtgaaga ttcaaaacca aataatacag atcttctgtt ttcttaaaat gcctacggtt
    11461 attgctgatc tctttataag gatcaaagct gtcttcttgt acccgatcta gcattcgtct
    11521 ttttttcttt aactttattg gttcatccag ctggtttcgc atccattcat attctgcaag
    11581 tgtcatgttc attcgctcta aaaatgatct caatgttaca aatgacattt ttttcactga
    11641 atttgtctca atcccgacta gagaagtctt aaaacagatt ccttccgcta attgggactg
    11701 tgtcaacttc ttgttgactc gaagtttctt gatcaattca ccatcataca taatagttct
    11761 cctttttaat ttaaattttt ccacattcaa taaatagata cgttaaaaag gaaaaataca
    11821 cgaaaaaaat aaaaaaatgt ttttttcata aaaaatacca accttcaagc attagaatgt
    11881 tgtctaacta ctgaaggttg gtttgattgt ttattgatta tttttttgct gttcaacgtc
    11941 tgttcttgtt actggatata agttggttgt tgttttatta ccattttttg aaaagacact
    12001 tgtcgaatgt tcgccttttt gcttttcgat tgcttcatta cgttccgctt catttttata
    12061 gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc gcaagagatt
    12121 ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct gatctttgga
    12181 gatttcctca tcgattcctg ctgctttcgt atcgatgatt tctcctgtct gattattgta
    12241 agcttttcct cctaggacgg tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac
    12301 tacttgatca tgctgagaag ataataagtc tgtaccaaaa tgaagataat ctttatcatc
    12361 gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccgt attcatgatt
    12421 gactgtccct ttagtataac caggcatatg gatcatcaat ggcactcgtt gcatctgtgc
    12481 gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcgcttgcta aatctttatt
    12541 atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat tttggtaaat
    12601 accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat aatgtgccgt
    12661 tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt cgaaattctt
    12721 gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt aaaacggctg
    12781 ttgtagatgc tcaagatatt gggcagattc tctgaataaa tctttatcta atatcccata
    12841 acctaacgtt tcgtcggatt catcaaaata agatcgatca aagaaattgt cataacctaa
    12901 gtttttatat acatgatctc tattccaaaa actagctaca ttcccatgga aaacagcgct
    12961 tgtatagcct tgcttttgtc caaggatcgc tggtgcggct tgaaagacat tgtctgaccc
    13021 aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca acatattttc
    13081 agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc
    13141 ttgattctgg aaaatactat ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt
    13201 aatcaaaaac tgctggaaac tttctaaatg gagaacgatc acatttttcc ctttagcgat
    13261 cccaaatgtt tcgggatttg gctctgcata atgtttcttt gtataatcta aaacttcatc
    13321 aatcccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt tgattccgtc
    13381 ataaatagta tacgcatcga ttcctagata tttgacaata tatgaacggt caaatgttcg
    13441 ctgcagcaat tgtggacggt tggcttcgct cagcgctaga tttcctgaaa aaataagccc
    13501 tgaaagacag gtaacagcga tagccatcgg tttgccgaca gggttgcttt tgataggtat
    13561 tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca gccagtagat
    13621 gacatcatgg ggcttcatca aagaaaagga gcttcctgac aatccttgcg acactttcga
    13681 aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa aaataatatt
    13741 caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatagcta acgctggctt
    13801 tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat
    13861 ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg catagtaggc
    13921 aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta aacgattttg
    13981 gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt ttttcttatt
    14041 ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt ttttattctt
    14101 tctaaagatt attccgtcca cttttcacag cataaaaccg ctgcgatgta agcgattaaa
    14161 ctaatgaaga gataaaagtc ttgtggtgac cgcttcactc cacggtattc aaaagccagt
    14221 ttctcggtat tcgtacaaaa aaagaggctg tgacaaaatt cttgtcgcag ctccttattt
    14281 cttgatgcag gacagctttt tcacaacttc ttccttaaac ggtgttctcc tgccaactcc
    14341 tcggccacaa gtcacaatat ttataaaaca tgagaagctg ttttttgaaa ttgttccttg
    14401 tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac
    14461 cctcggtatt agattatgtc aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa
    14521 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tataaggagc tattttttga
    14581 aattgctcct tgtggctcag agttaaacgg caggctcaag acctcttgat a
//
LOCUS       DAFFFQ010000074        14496 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000074
VERSION     DAFFFQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.074"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            136..456
                     /locus_tag="LQB69_002274"
     CDS             136..456
                     /locus_tag="LQB69_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526417.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            908..1108
                     /locus_tag="LQB69_002275"
     CDS             908..1108
                     /locus_tag="LQB69_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3526418.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1255..4044
                     /locus_tag="LQB69_002276"
     CDS             1255..4044
                     /locus_tag="LQB69_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL3526419.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4091..4618
                     /locus_tag="LQB69_002277"
     CDS             4091..4618
                     /locus_tag="LQB69_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL3526420.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4639..5829
                     /locus_tag="LQB69_002278"
     CDS             4639..5829
                     /locus_tag="LQB69_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3526421.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5869..7167
                     /gene="asnS"
                     /locus_tag="LQB69_002279"
     CDS             5869..7167
                     /gene="asnS"
                     /locus_tag="LQB69_002279"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL3526422.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8343..9017
                     /locus_tag="LQB69_002280"
     CDS             8343..9017
                     /locus_tag="LQB69_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL3526423.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDNIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9014..9355
                     /locus_tag="LQB69_002281"
     CDS             9014..9355
                     /locus_tag="LQB69_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526424.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9385..9744
                     /locus_tag="LQB69_002282"
     CDS             9385..9744
                     /locus_tag="LQB69_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL3526425.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            10269..12353
                     /gene="cadA"
                     /locus_tag="LQB69_002283"
     CDS             10269..12353
                     /gene="cadA"
                     /locus_tag="LQB69_002283"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL3526426.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            12644..14110
                     /locus_tag="LQB69_002284"
     CDS             12644..14110
                     /locus_tag="LQB69_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3526427.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4800 a         2510 c         3102 g         4084 t
ORIGIN      
        1 agaggttgtg acgtggtcac gacctctttt tgacgattat gatacaaaaa acatcattta
       61 tgaacaaaat gtgaataacg ggcgttcttg agatatgatt ttaattggga aaaacttatc
      121 gataaggagg tctttatgaa aaaaagactg ttatggtgtt tgctgtttct atccctaagt
      181 ttaacaagtc agccagtttt tgctgaagaa acacagcggt ctcctattac aggcacacgt
      241 ctgtacaaaa atgtttatgt acagacaaca gatcttgatt ctgcgattcc tgaaacagaa
      301 gaacggtctt tgcagccaag atttggttac ttttggtact gtccgacctg tgattatgaa
      361 agttgctggc atttcttaca ttctacagct gcagcttttg cttttactca tactcagaaa
      421 tatgatggac atcatcctgt tatttatgag gtatagatat gagcaaaaaa gaaaaactaa
      481 tctcgttgat cgtattggtt tgccttgttc ttctggtaac tgtcataggc tatcagaaat
      541 accatgagaa catcagacct actaaagaat cttccccagc ctcgaccgtt gaaacagaac
      601 cgataagtag tcagaaagaa taattttttt agtttaaaga agaacatcag agcaaaaaaa
      661 caccaactga acaatatgtc gtttatttta aaaaaagttc ggtttttcac tttatgtaat
      721 cgctgagaaa acttttttta ataaaaaaag tcgaaaaact ctagcttttt tgtaaacggt
      781 atgatatact ataggagtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
      841 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
      901 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
      961 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
     1021 caaaacttta gaagaaggcc aagcagtaac tttcgatgtt gaagaaggcc aacgcggacc
     1081 tcaagcaaca aacgttacta aagcttaatt aacctatact aaaaaataaa cgcccagaca
     1141 tttgcgtgtc tgggcgtttt tgtctatttt ctttaaaaag cgatgagagt atttttctgc
     1201 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
     1261 ccaagaactt atgcagtcgt tgatatcgaa acgactggga cgaatccaaa agaagatcgg
     1321 atcatccaat ttggctgcgt catgattgaa tcaggtcgga tcacttcacg tttttcgatc
     1381 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
     1441 caacgcgtcc aaaaagcgcc ttatttcgaa gatgtcgccc aaacgattta caatcttttg
     1501 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
     1561 ctcatgcgat gcggacttcc tagtttgaag ataccaggca tcgatacagt agaacttgca
     1621 caaattttct tgccgacgga accaagcttt cgcttagctg atttgtcaga aagccttgga
     1681 tttattcatg agaatcctca tcaggcagat agtgatgccg aagtgacggg tcaacttctt
     1741 ttgctgatcg aagaacggat gagaaaattg ccaatcatca cgttagagca gattgcgcga
     1801 ttaagtagac acacaggtat ggatacgagt cgtttcattt atcatgtgat tgaagaaatg
     1861 aaagaaaaac cagagccact tgatccctca ttagaaatcg tcgatgggct agctttgcag
     1921 aaaaaagaag tggaactatt cacatccgtc cattatggcg agcggacata tccaagaaaa
     1981 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacaga
     2041 ctgatgaatg cggtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
     2101 gcagctacag gaatgggtaa aacaatcggt tatctgctgc cattgagtta tttggctaca
     2161 cctgaaaaac ccgctgtcat cagtaccgtt tcgctagttt tgcagcagca gatcatagaa
     2221 aaagacattc cattgctgaa tcaattgcta gatcagccca tacaacctgt aattatcaaa
     2281 agctatcgcc actatattga tttgcagcgt tttaaaggca cacttgtgga accgccagaa
     2341 caaaaacaat atgctttgta tcaaatggcg gtcttagttt ggctgacgca aacgaagact
     2401 ggtgatttgg atgaattaca tttaacaaat ttgaaccatt cattctttgc agatatcgcg
     2461 cacagaggta ttggattttt agcaagaaat cagtcatttt acgaacaaga ctttgttcgt
     2521 ttcttgcaaa agaaaatcag gcaaagtaat tttttgattg tcaatcatgc gtttttagta
     2581 caagaaacac agcgaaaaga accattgctt ccagctagtc catttttgtt gatcgatgaa
     2641 gtacatcatt tgccggaaat cgctgaaaaa gtaaatgaca aacgattaag tattaactat
     2701 ttccataaac aaatccagca attagaagaa ccaaatcagc tgtttgaccg aatacaaagc
     2761 tatttaccga aagaacatga agccttacgc tggttatcga tttatcgaga agaactttct
     2821 gcgctatgcg aagcacagat ttgtatggca gaaggattaa tgagtttagt gagcggcaag
     2881 caacctaacg gacagtatcc agaagaagtc atgatgacaa aagagatgtt ggatcagctg
     2941 ccactggaat ctgggacagc cattgatcac ctgcagcttt tttatcagga gatccagcag
     3001 ctacagtcac aaatgcgtct gacttttgag attatccaag aaagctggac aaaccagatg
     3061 cggatcgatt tagcaagttt atatagccta tttgatcaat tagacaagca atacttgctg
     3121 atggatcaat ggctggaaga ctggcgagat catttgatcc attggttcgt tcctgggaac
     3181 acagcgcagc agatgatttt tcatctccat gattttcaag cggctatgtt gccgtcgact
     3241 gtttggtatc ctcgatatga tcgtattctt tatattggag gaacgctgaa gatcggccct
     3301 aatcgacact atctctctaa gaggcttggc attgaagcag cacctcttaa agtgattgcc
     3361 tctccttatg attatgagaa acaggcaagg atctatgtgc caaaagaggc acctgccgtc
     3421 catcaagtca gcaatcaagc atatatcgat tatttagaga cagcacttac aggtctgatc
     3481 gaacaagaag atcggccaat acttgtgtta ttcacttctc atgatgtttt gcagaaggtc
     3541 tatcagaaaa tgcatctgtc tatgctagaa aaaggacgag aaatactagc tcaagggttt
     3601 ggtggaagcc gggagaagct tttgaaacgc ttcattttgt cagaaaaaag cgtattgttt
     3661 ggcgctgaca gcttttggga aggtgtagat ttgcctggag atgctttgca gatcgttgta
     3721 gtcacacgtt tacccttcga taatccgctt cgcccgatgg tcaaagctag gaacgcttat
     3781 ttagaatcag aggggatcaa tcctttttac caggaatccg tacctaaagc agctttgaag
     3841 ttacgccaag cattaggtcg cttgataagg acggagacag atcgtggcgt attgcttatt
     3901 ttggatcgaa gactagtcac tgcaaaatat ggcgcacgga tcatccatgc attaccgaaa
     3961 aaagtaccga tcaaagagct agctatggag gaaatctatc aggatatcca tgaattctta
     4021 aagaaaaatg aagaaggaag ttagaggagc tgtctttttt cataattaaa atctgctata
     4081 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
     4141 aacgattaat cgcgtgttac ttggcttgat cgtattttta ttagcggtca tcgtgatcag
     4201 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtgcag gcgaagaaag aagcagtaga
     4261 aatcgcaaag aaatatgcaa atcttgagga aaccgatcat ttttattggt ttactcgaaa
     4321 gaagacatac ttttcactga caggtacaaa tgataaagga aaagaaatcg ccgttatcat
     4381 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
     4441 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
     4501 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
     4561 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
     4621 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
     4681 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
     4741 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
     4801 gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
     4861 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
     4921 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
     4981 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
     5041 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
     5101 gaacagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
     5161 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
     5221 aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat
     5281 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaac
     5341 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt gggaaatcct
     5401 gtgatcatca atggaatgat cgctgttgct tcccaatcaa ctagcaatcc gacagcagtc
     5461 agtcaatatg cagctgttga agcattgaaa ggtgagcaag atacagtaga agaaatgcgg
     5521 aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt
     5581 tctcttgaaa agccgcaagg agctttctat ctttttccta atgtaaaaga aacgctacgc
     5641 atgtgtaagt atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
     5701 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact
     5761 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
     5821 caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
     5881 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
     5941 aataagcgat caagcggaaa aatcgcattt ttacaattaa gagacggaac agcctttttc
     6001 caaggtgtag ttgtaaaaag cgaagtgtcg gaagaagttt tccaaacagc taaaaatcta
     6061 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
     6121 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
     6181 acaccaaaag aacacggaac agatttcttg atggatcatc gtcatttatg gctgcgttct
     6241 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
     6301 tttaatcaac atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa
     6361 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
     6421 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
     6481 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
     6541 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
     6601 ttcttagtac aaaatgtttt agatcattgc gaatatgcat tagatgtact aggacgagac
     6661 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta cgatgatgca
     6721 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
     6781 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
     6841 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtaatttgt
     6901 gcagatatga tcgcaccaga aggttacggt gaaattattg gcggaagcga acgggcaaca
     6961 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
     7021 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt
     7081 gaacgaacag tcacttggat tgctggtatc gaccatgtac gtgaagctat tccattccca
     7141 cgtttgttga accgaattta tccataagag gtgatgaaaa agctgttctg catcaagtaa
     7201 ttagaacagc caagcaaaaa cagctgttca tgtttttgaa ttggcgggac ttattaccgc
     7261 agatgtaagc ttttgaatac cgtttagtgg agaggttgtg aaaaagctgt ttagtttcga
     7321 gtattaagaa aaattttgtg gaaatggctt tccacatttt tgcataaatt tggcttaata
     7381 ccgagaaact ggcttttgaa caccgtttaa ggaagaggtt gtgaaatcag tgccttgacc
     7441 tgagtattag agaaacaaaa cggaaaaata gcttctcata ttttaccgtt tttttagcta
     7501 atatcgaggg tcagctgatt gaacaccgtt tatatcaaga ggttgtgcgc ctgccgttca
     7561 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7621 cttgtgaccg aggagttggc cggagagcaa cgtttattta aaaaaaagaa ctgcgaactc
     7681 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7741 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7801 atacaaagaa ggttattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7861 agcagcatct agtacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7921 attttcagca tgcaatgatc catatgtaag tttaattcat ccttgaaatc atcggaccag
     7981 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa aagaacattt
     8041 actggaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     8101 tgacggaagc ttaggtgtga tcgatttctc tggaccaaac aacgaaggaa tcaaggggaa
     8161 tccggcacgt taagaaaaaa agtataaaaa actagacact tgatgagcgt tcattcaatt
     8221 gattgaaggg actaggctgg tgtcattttt tatacatata ttttctcttt cgtcttgata
     8281 gggcttatgg gataatataa ggaagaaaat catcgtttta ttcaaaaagg agtttttgac
     8341 aaatgaaaaa acgtatatta tttatcggtg acagcattac agacgcagga agagaccgag
     8401 cagaagcaac tgatctaggt aaaggttatc cacttttgat tgccggaaaa ctaatctccc
     8461 aatatccaac gatgaaattt gaatgcatga accgtgggat aggaggagac aatattgcag
     8521 atctgcaagt acgctggcaa gaagattgtt tagctttaca acctgactat gtatcgattt
     8581 tgatcggaat caatgatacc tggcataata ctagcgatca ggctatattc ggaactgaga
     8641 aatctgcgca gcaatttgaa tccgtttatc gaaaattact aaatgaactt caaacagcgg
     8701 aaatctgcaa catcatcttg atggagccgt ttgtacttcc tataaccgac gaccgtcatg
     8761 gctggcgtaa agacttagac cctaaaatcc aaattgttcg taaacttgct aaggaatatg
     8821 gcgcattgct ggttccttta gatggcatat tcaatgcttt agggattgct tatggttttt
     8881 cgaaatatac aagagaagat ggggtccacc caacgattgg cggacatgag atcattgcac
     8941 aaagctggat agatgcaatt gccgcagtcg atttttttag tggagataaa aatgagcgaa
     9001 agaaaagtga gaaatgatgg aaatacggtg ggcagtaaaa gaagatttac ctcaaatcaa
     9061 tcagctgatt actgatcatc cagaatcatt cgtaaaagca aaaacggcag ctgacctaaa
     9121 tgataagaat aaactattgc tcgttgctgt aacaaaagaa aaacggatta tcggatttgc
     9181 gcaagcggac gtgcaacagt cagaagaacg ggtggatcag cgaccatcag gaaaactaat
     9241 ccaacacaca ttagtaacaa aagaaaatac ggaagaaatc caaaaaaaat ttgcagaata
     9301 cttgatgctg gaagctaaaa aaagaagaat aaaatcaatt tttgcccagt tttaatcaaa
     9361 aacagtaata gaaggagaaa tacaatgagt tgtttaggaa atgtcatctg gtttatattc
     9421 ggcggactaa tcggtggtct tagctggtta gcagcgggga tactttggtg tatcacgatc
     9481 ataggtatcc caatcggtat gcaatgtttt aagatcgcct ccctttcttt tgcgcctttt
     9541 ggcaaagaag taatttatca ccagtctggc atgtcattga tcgttaatat catctggctg
     9601 atcgtttcag gcctaccaat tgctctcgca catgtcgtca gcggcatcct tctatgtatc
     9661 acgatcatag ggataccttt tgccaagcaa tctttcaaac ttgctcgatt ggcgttgatg
     9721 ccattcggtg cagaaattgt ataaaaagag aataataaag gttagaaaac gcatagagtc
     9781 aggtgggata catgtatttc acctgactct atgtgttttc ctgtataagt tctcccatgt
     9841 gacttttacg tcttttattt atatgggatt actgtttatg gaaagttgaa ataattttca
     9901 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9961 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
    10021 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
    10081 gttggataag agcaggggca atgatcgaag agaaaaagaa agaataagcc aaaacatctg
    10141 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10201 tgaataacca ttcatttata attagttatg aagagatgtt catatgttga ggcgaggagg
    10261 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10321 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10381 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaacaaaaa
    10441 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10501 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10561 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10621 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10681 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10741 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10801 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10861 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10921 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10981 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    11041 ttggtgaaac agtattaagc ggatttatca ataaaaataa accattttta gttcaagtag
    11101 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11161 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11221 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11281 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11341 tgatttccgt gccacttagt ttctttggcg gtatcggagg cgcaagtaaa atcggtgtat
    11401 tagtaaaagg ttcaaattac ttggagttgc ttgcgcaaac cgagacagtg gtattcgata
    11461 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11521 caaagaaatt catgcaatat attgcgagtg cagaacagtt ttctacacat ccgattgctc
    11581 aatctgtttt agaaggctac gcaggtccgc ttttaccaac aactaatatc caggaatttg
    11641 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11701 tgatggaaag atttgaaatc tcttttcctt taagtcaaga aatcggtaca ttgctttatc
    11761 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11821 ccgtagacgc attggttcaa ttaaaacagg ctggtgtgaa gaacacagtc atgttgacgg
    11881 gcgactcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11941 gcgaattgct tcctgaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    12001 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    12061 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12121 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12181 aaatcgtcaa acaaaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12241 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12301 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa agaggacgaa tgaacgtcct
    12361 ctttttttta tatccttttc ttaatgaaaa atgtaaagaa aatagatagg attataaaat
    12421 aactaatgtt tttaattttg cagaaaattc aaaatagctt gaaattcaat aaggtcaatg
    12481 aaataaaaga aaataactta tttttttgat ggaaaaaata tgaaaaaacg atgtgtatta
    12541 aataaaagaa tacacacata aaataacact gtcttgtaag tttgttaaaa attgaatatg
    12601 attacatctg gataacaaat ttttatcata aaggagagaa cgaatgaaca acaaacaatt
    12661 acgctggttt actgttgcgt taattgcatt taacatggtt tggggcatgg ggaatgtagt
    12721 taataactat gctcagcaag gagtatcagt tgtcacctct tggctattga ttttggccat
    12781 ttattttatt ccatacgcat tgatcgttgg acaattagga tcagccttca aggatagtaa
    12841 aggaggcgtc agttcatggg tcgaaaatac gacatctaat aaacgactgg catactatgc
    12901 cgcttggact tactgggtcg tccatattcc ttatttggca caaaagcctc aagcaattct
    12961 gattgctggt ggttgggcag tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca
    13021 aatggtagca gttatttcac ttattatatt tcttgccttt ttatatttat caacaaaagg
    13081 actttctact ttgaaagtga ttggcggact ggccggaaca gcgatgttcg tcatgtcgat
    13141 cttatttatt cttttagctg taacggcacc gttcatcaat ccaacgatgg aatttgccac
    13201 accacatatg aatcaagtga aaacatatat tccaacattt gatttcagtt atttcacaac
    13261 cgtttcgatg ctagtatttg cagttggagg ggcggaaaaa atatcccctt atgtcaactc
    13321 tacaagaaat cctgcaaagg aatttcctaa agggatgatc ttcttagcag caatggtagg
    13381 gatttgtgct gtattaggct cagtcgcaat gggtatgctg tttgctagtg ataatattcc
    13441 taaagacttg atggcaaatg gggcatacag tgcttttcaa attttaggta attactatgg
    13501 tatcggtaac tcattgatga tcatttacgc attgacaaac atgattggac aaatctccgc
    13561 attagccttt tcaattgatg caccactaaa aatcttgtta gcagatgcgg atccagaatt
    13621 tgtaccaaac tggctacgta aacgtaatcg taaaggtaca ttggtgaatg gttatcttct
    13681 gacaggtata ctagtaggaa tcattatttt gttgccgatt tttggtatca aagatatgaa
    13741 tgaattagta aaatggttga cgaacttgaa ctcagttgtc atgccaatgc gttacctatg
    13801 ggtgttcttg gcatatatga tgttgaataa agcttataag aaattcaatt ctgaatataa
    13861 atttgttaaa aatccaaaaa tcggttttat ctttggtgct tggtgctttg ccttcactgc
    13921 gtttgcatgt atcctaggaa tggtgccaaa agtagattat actgctgatc ctaaagcatg
    13981 gtggttccaa ttatttacga atatcatcac accaatcgtc ttgatcttat taggtatgat
    14041 cttgccagca attgctcgtc gagacaaaaa caaagaaatc actgaaccag aaacagtggt
    14101 tcagccttaa aatagaaata cttgttacaa gaggttgtga cagaagtggt cagcttcaag
    14161 aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt
    14221 tcgaagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc
    14281 aagtaataag aacagccaaa caaaaatagc ccctcatatt tttcgtttgg ctgggcttat
    14341 taccgcagat gcaagctttg gaacaccgtt taaggaagag gttgtaaaat cagcgatttg
    14401 acctgagtat tagagaaaca aacggaaaaa tagctcctca tattttgccg ctttttgagc
    14461 taatgctgaa ggtcagctga ttgaacacca tttatt
//
LOCUS       DAFFFQ010000075        14375 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000075
VERSION     DAFFFQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.075"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            142..523
                     /locus_tag="LQB69_002285"
                     /pseudo
     CDS             142..523
                     /locus_tag="LQB69_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            654..1604
                     /locus_tag="LQB69_002286"
     CDS             654..1604
                     /locus_tag="LQB69_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3526428.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2122..2577
                     /locus_tag="LQB69_002287"
     CDS             2122..2577
                     /locus_tag="LQB69_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3526429.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2918..3520
                     /locus_tag="LQB69_002288"
     CDS             2918..3520
                     /locus_tag="LQB69_002288"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526430.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3614..3946
                     /locus_tag="LQB69_002289"
     CDS             3614..3946
                     /locus_tag="LQB69_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HBL3526431.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4097..5076)
                     /locus_tag="LQB69_002290"
                     /pseudo
     CDS             complement(4097..5076)
                     /locus_tag="LQB69_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5081..5848)
                     /locus_tag="LQB69_002291"
     CDS             complement(5081..5848)
                     /locus_tag="LQB69_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL3526432.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6338..6763
                     /gene="lacA"
                     /locus_tag="LQB69_002292"
     CDS             6338..6763
                     /gene="lacA"
                     /locus_tag="LQB69_002292"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL3526433.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6780..7295
                     /gene="lacB"
                     /locus_tag="LQB69_002293"
     CDS             6780..7295
                     /gene="lacB"
                     /locus_tag="LQB69_002293"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL3526434.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7306..8238
                     /gene="lacC"
                     /locus_tag="LQB69_002294"
     CDS             7306..8238
                     /gene="lacC"
                     /locus_tag="LQB69_002294"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL3526435.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8241..9221
                     /gene="lacD"
                     /locus_tag="LQB69_002295"
     CDS             8241..9221
                     /gene="lacD"
                     /locus_tag="LQB69_002295"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL3526436.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9249..9566
                     /locus_tag="LQB69_002296"
     CDS             9249..9566
                     /locus_tag="LQB69_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL3526437.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9566..11272
                     /locus_tag="LQB69_002297"
     CDS             9566..11272
                     /locus_tag="LQB69_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526438.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11414..12820
                     /gene="lacG"
                     /locus_tag="LQB69_002298"
     CDS             11414..12820
                     /gene="lacG"
                     /locus_tag="LQB69_002298"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL3526439.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12843..13115
                     /locus_tag="LQB69_002299"
     CDS             12843..13115
                     /locus_tag="LQB69_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HBL3526440.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13136..14035
                     /locus_tag="LQB69_002300"
     CDS             13136..14035
                     /locus_tag="LQB69_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL3526441.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4695 a         2371 c         2771 g         4538 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgatgacact atcgttcttc atcaacgtaa
       61 gttagatttg ttgaaagagc agaaaaaagg atttctacaa aaaatgtttg tttgatattt
      121 taaatagaaa agagagataa agtgaagttt ttaaattttt tgaactataa atatttaaac
      181 tgggtattat atggtattac attatttttt attatttatt acttttttac cgatgatgat
      241 atttttttaa ttttagcaat actttttctt gtttcatcta attttagtga taacttaatt
      301 gaagttctta cagaaccaga aattcggtct atgacggatg aagaacaaca gattattaaa
      361 gctatgattt cggaaaataa gaaagacgta gtagttatca aaaagattag agagtataca
      421 aatttaggtg ttttacaagc caaaaaagta tatgaaaaaa aaaaaaaggg tctataatta
      481 caaattagat aatacattag aagctggaga caagattgtc tagataatgt gtgagataaa
      541 atttttggca ggttatcata cataagacaa aatgttttta aaactgataa cgtatttatt
      601 ccacaacaat tacgcattta tttctgagaa aaatataaag caggaggatt acaatgctga
      661 aaacttattc cttaacaaaa gatacattat tagtttcgtc agaatcaaat atcaaaaatt
      721 cttctgttgt ttatgtttat ggtgacgaac gagaatatat cgaatcttct gaacataaat
      781 atgactttaa aataggcaat atactaacgt ttgatgatcg agttgcatat gacacgatga
      841 tagataagaa cgatgaaaaa agtttgctcg taagtttttt gtttcccgag gtacatgaag
      901 gtgggcatct agataccctg acgacatctg ttgttttctt ggtatttaaa aataagttga
      961 taatttttac aaaacaaaaa ttaaaattca ttcccgaact cttatccaat atggttttac
     1021 gtgatgtcaa tcatttcgtg gaggaagtac ttttaaagat catgcaaaaa gatttctata
     1081 tgatgcttaa cgaccttcgt ggagtcaagg aaaaaattga tgatttagac tcagaaatat
     1141 caacgtccgg ttcactcaaa ccaacgtttg gtgatttatt gactcttcaa aaatacatga
     1201 tagctttgtc aacaacttat aaagctaacc ataaagcgct tgatttcatt aaaaaaaatt
     1261 tcacaactat tgacgatatt gattttagta ctaaaaaaat aattgatgat ttatacgata
     1321 caagtgatac aatggataga atcattttaa gttacagcca atatttggac aaattggaaa
     1381 atatgataaa caatatgatt tcgtatcagc taaacatgat tatgaaaacc ctaaccgaga
     1441 tatcaattgt tttgactatt ccagcaatta tttttggctt ttggggaatc aatgtccatg
     1501 tcccgtttga aaagtcttct tatggtgttt tagccgtatt gattatctct gctatactaa
     1561 gtctaatttg ctggcttttg atgcgacgca aaacatattt gtaaagtatt tgtgataccc
     1621 taatatgatt taaagcagaa tttctttgtt gactttttaa aaggctgatt cctttcaata
     1681 atttaatagc ttcagcggct gctcagaagt caataaattg gactattttt attcagttac
     1741 ttaggcaaca tttgagatac gattgcgatt ataaaaattg atataccaac caatggctgc
     1801 ttggaagact gacatattag cctcgatatc atcgaataac taaatacaga aaaatacaat
     1861 aaatattatc aaactaggga ctatactaga aaacatcagt acattcctta actcagggtc
     1921 tataattaga aaatcgcccc tttgaaaact gattaaaaca gcccccatta tctagtgact
     1981 agataatggg ggctgttttt ttgttaagtg atataataaa ccataaaaga agtttagaaa
     2041 tacggtcctg gtcatcaata aataaacaat ttaattaatt atttatttaa ttatttaatt
     2101 tcagaaaggt atacatgaat catggtgcaa caaattgtcc tacccatcaa agactccagc
     2161 gtcttaaaga tggtacaaga tacacttctc gatagtttcc gtgctggtcg ccgaaattac
     2221 accgtttttc aagttggtaa ggccacgcta ctacgtgtga gtgatgtcat gacattaaaa
     2281 aaatcagatg tctataatcc agacggctct gtcaaaaatg caacctttat tcatgataaa
     2341 aagaccggta aagcaaacac gttatattta aagcctgttc agcaagactt gttgcaatat
     2401 catgattggc tggcccaaga aaatatcaat tctgagtggt tatttccctc gacagcccat
     2461 tatgatcttc atatcactga gaagcagttt cataaggtga tggcgcatgt tggtgatctc
     2521 ttaggaatca acttggaaga caaagagaaa aaattgcgag tcagaaaacc gttttgagat
     2581 atgaacagca tttgccttca cgccttagcg attttaaata atagccttga aagtgattat
     2641 ggaaaagccc tctacgctca atgcaaaatt gacgtagaaa ctatttttga tcgattgaaa
     2701 ggtgtatttg gtatgtgaag aacccatgtc agaggcaaga acgttgcacc tactgacatt
     2761 ggaatgatgc ttatgagcat gaatctaacc aaattatccc ttgaggctag aagaaaagcg
     2821 gaagcttttc aacacaaagc agccaaaaat aaaaaacgcg acgaagcaat cacatttatg
     2881 attattcatc gcgtttttta tatttagagc tagttatttg ctagacccac tatttacaga
     2941 tgaaacgttt atcacctttg gttttgggca tttagtggaa ttagcagaac caggtcatta
     3001 tgacgaaaag tggcaaaatt ggaaacttga atctttgccg atttttcctg atcgatacga
     3061 ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgcagaac ttctcaaaaa
     3121 ggcaaataca attattgttg caacagatag cgacagagaa ggtgaaaata tcgcctggtc
     3181 gattatccat aatgcaaatg ccttttcaaa agataaaaca tttaaaagac tatggatcaa
     3241 tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag gaatgaatta
     3301 ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga tcggcatgaa
     3361 cgcaagccct ttgtatacgt taaatttaca acagaagggc gtacaaggta cattttcact
     3421 aggacgtgtt caaacgccca ccttatacct tatttttcag cgccaggaag ccatagagaa
     3481 ttttaaaaaa gaaccttttt tcgaggtgga agctaagtaa aacaagggtc atttaagggc
     3541 gttctaagcc ccacacagcg ttttaaaacc caagaggagc tttttagctt ttgtttcttc
     3601 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagaa
     3661 gaaaacgaat agtccgagtt cgttttcttt aagtagtttg caatcaaaag tcaatcagct
     3721 ttataaagcg acagcgagcc aaactttaaa agctatgcaa ggactgtatg aagcaaaatt
     3781 attgagttat ccaagaacag atacaccatt tattacagag aacgaatttg cttatttaaa
     3841 agcgaatttt ggcaaatata gcggtttttt aggacttgat cttgaaatgt tgttatcatt
     3901 gataatgata cgttcaaaca acagcaccca aattttgacg aattagtgaa actctatgaa
     3961 aaagacgtag taaaacatgc aacatcctat tctaatcagc tggtaaagct taattaggtt
     4021 atataagctt gaaaataaag ggtttgtcca atatggacaa gcctttttct tatgttttgc
     4081 cacagatttg ccacacttat tgtgtaagag tatcaaggat aggtataatt tgatactttg
     4141 tttttttagt gatgtgcaaa taaatttctg ttacacgact acctctagaa tgccctacac
     4201 ggtcttgaat tgcttctagg ggtattcctt actctgctaa gaaactaata tgcgtatgac
     4261 ggaatatatg ggtcgtaata tgtttattaa aactaagttt attatcaata tcaattcctg
     4321 ctcgttttgg attacctttt cgatttaatt ttccattttc aggtgagttg aggaaattgt
     4381 taaggacact ataatgaaga ggtgttccat tatagtcggg tttatgacga cttttggaag
     4441 taaaaatcca tccattatct ttataatcag gattgaatct catatgttgg tgatttaact
     4501 caatttcctc tttaagaatt tctatagccc ttttagataa ttggacattt cggtaacttt
     4561 gtaagttttt tacattacct tcatagctat tttctgcagc tcctccgtga gtggactgac
     4621 taatgatgat attttgcttt tcaaaatatt gataggcacc aatattttgg agtttcgact
     4681 ctgtaagtca acagggccat tagttagaat acctattggg atgcggtttt tatgtagagc
     4741 cagcagtagt tgatgtatct caggaatcat cgtaatctga tcgagttcat attcatagtc
     4801 agcttggaag tctagagctt ctgtcctaga aatactatcg atgccaaatt cttccatagt
     4861 cttctgacag cgaaaaattc taagctgctc aatactgatg agttttttcg tgtatttagg
     4921 aaaagcaaca tcagaccaat atctgaaacg tttgtaaatg ttatctattt gttggatatc
     4981 aatcgttgga aaacatttct caacgctacg tttaaagggg agttgatggt cataaagagt
     5041 gtcatcaagg tcaaaaacaa tatttttata agtcataatt ctagtccgct tttaaaatag
     5101 tagtgtactg gctaaattcc tctaagtgtt gagggctaat ctggttatct gtaatgatgg
     5161 tatcgagttg atctagatta tagaagttaa agaaatcgta tcgatcgaat ttagtactgt
     5221 ctactaataa gaatttttct acagcattgt ttagggcaag ttgttgaatc acaccttcct
     5281 tgtcactata tgtagcaata gaattatggg taacagcatt tgcactaaca aaagctttgg
     5341 caaatctaat tgcttttaaa tttgtcgaag ccattgaacc tacaaaagct ccagttattt
     5401 ctctatattc accgccaaga agcaataaat caatggtttc gctatcattt agaatcaaga
     5461 acacagggag actatttgta atgacacgaa ttttataacc tttacgacct ttcaattcta
     5521 atgccagttg ttcaagtgta gttccaggtc caataaaaat agtatcgcca tctttgatta
     5581 aagagcaggc ttttttagca atgtcaattt tttcttttgt atttagactt ttcttctcga
     5641 tatgtgtctt ttcaagtggc tttttagagg aaagcaattg tgcaccacca tgagtacgtg
     5701 ttaaaagtcc atcagcttct agagcatcaa ggtctctacg ggctgtcata tcggaaatat
     5761 ctagttcatc tattatttct tttatggtta tggtcccatc aatctttaac atatctaaaa
     5821 ttttttctaa tcgtcgtttt ttgttcatat gaagaccctt ctttcataaa gtaatttttt
     5881 tccaaagata attctctttt aattgtatca taaaagacaa tattttcaag gtaaaacaaa
     5941 caatttcaaa caaaaacaaa tgttagatga tgaaataaga acagaggatt gacgtatatt
     6001 agcttaggtc agattttgta taagacgaaa ataaagtagg acctcttaat cagtaagtta
     6061 tagaaagtaa aagacttttg taatacttga atagatattt cacgtccatt ttgtgatgga
     6121 ttaaatgaac aaaaatgaac aataatttaa cggtgttatc tattttttaa aaaaacaaat
     6181 aaaaaaaaca aaaaataaac aaaaaaagtg ttgctttttg tttgaatgct tgatattata
     6241 taaacaaaga aatgatgaaa acgttttttc gaacattttg caaaatgttt tctacttcta
     6301 cgtagcattt ctttctaaaa tttaggaggt ggtcaaaatg gctattgttg ttggtgcaga
     6361 tctcaaaggt acgagattaa aagatgttgt gaaaaatttt cttgtagaag aaggttttga
     6421 ggtaattgat gtgactaagg acggacaaga ttttgttgat gttaccttgg cagttgcctc
     6481 tgaagtaaat aaagatgagc aaaacttagg tattgtaatt gatgcttatg gagctggtcc
     6541 attcatggta gcaactaaaa ttaaaggtat ggttgcagca gaagtttcag atgaacgctc
     6601 agcttatatg acacgtggac ataacaattc acgaatgatt acggttggcg ctgaaatcgt
     6661 cggtgatgag cttgctaaaa acatcgctaa aggtttcgtc aatggtaaat atgatggcgg
     6721 acgtcatcaa atccgagtag atatgcttaa caaaatgtgt taagaaaagg agttaaggaa
     6781 tgagaatcgc aattggatgt gaccacattg tcacagatga aaaaatggca gtatcagaat
     6841 tcttgaaatc aaaaggacat gaggtccttg atttcgggac atatgaccac gcacgtactc
     6901 actaccctat ctatggtaaa aaagtcggtg aagcagtagt aagtggtcaa gcagacttgg
     6961 gagtatgtat ctgtggtaca ggtgtcggca ttaacaatgc agtcaataaa gttccaggtg
     7021 ttcgctcagc gttggttcgt gatatgacat cagctatata tgcaaaagaa gagttaaatg
     7081 cgaatgttat cggtttcggt ggaaagatta ctggtggtct tcttatgaat gacatcattg
     7141 aagctttcat tgaagctgag tacaaaccaa cagaagaaaa taaaaaattg attgcaaaaa
     7201 tcgaacacgt tgaaacacat aatgcacatc aagcagatga agatttcttt acagaattcc
     7261 ttgaaagatg ggaccgtggt gactaccacg attaaggtat aacaaatgat tctgacagtc
     7321 acactaaatc cttcagtaga catctcttat cctctagaaa cattgaaaat tgatacagtg
     7381 aatagggtga aagatgttag taagacagct ggtggaaaag gcttaaatgt tactagagtc
     7441 ttgtatgaat ctggtgataa agttactgct acaggtttct taggtggtaa aattggtgaa
     7501 tttattgaaa gcgaattaga acaatctcct gttagtccag ctttttataa aatctcagga
     7561 aacactagaa actgtattgc tatcttacac gagggtaacc aaacagaaat tttggaacaa
     7621 ggaccaacaa tttctcatga agaagcagaa ggctttcttg accactatag caatcttatt
     7681 aaacaatcag aagttgtgac aatttcagga agtttacctt caggacttcc caacgattat
     7741 tatgaaaaac ttatccaact tgcttcggat gaaggagtag cagtcgtttt ggattgttca
     7801 ggtgcacctc ttgaaacagt tctaaaatcg tcggctaaac ctactgccat caaacctaat
     7861 aatgaggaac tttcacagtt gcttggaaaa gaagtaacaa aagatattga agaactcaaa
     7921 gacgttctta aagagtcact ttttagtggt atcgaatgga tagtggtctc attaggtcga
     7981 aacggtgcct ttgcaaaaca tggtgatgtt ttctataagg tagatattcc tgatatccca
     8041 gttgttaatc cagtcggatc aggggactca acggtagctg gtattgcatc agctttaaat
     8101 agtaaaaaaa gtgacgctga cttattaaaa catgcgatga cattgggtat gttaaatgct
     8161 caagaaacaa tgacagggca tgtgaatatg actaattacg aaacactaaa ctcacaaatt
     8221 ggagtaaaag aggtataaaa atggtactta cagaacagaa acgcaaatct ttggaaaaaa
     8281 tttcagataa aaacggtgtt atctcagctt tggcatttga ccaacgtggt gctttgaaac
     8341 gtttgatggc acagtatcaa gatacagaac caactgtagc tcaaatggaa gaactaaagg
     8401 ttttggttgc tgatgaactt acaaaatacg cttcttcaat gttgcttgac cctgaatatg
     8461 gacttccagc aacaaaagca ttggataaag aagcaggtct tcttcttgcc tatgaaaaaa
     8521 caggttatga tacatcaagt acaaaacgtt tgcctgactg cttggatgtt tggtctgcaa
     8581 aacgtatcaa agagcaaggc gctgatgccg ttaagttctt gctttactat gatgtagaca
     8641 gctcagatga acttaaccaa caaaaacaag cttatattga acgtgttggt tctgaatgtg
     8701 tcgctgaaga tattcctttc ttcttggaaa tccttgctta tgatgaagaa atctcagatg
     8761 caggttctgt tgaatatgca aaagtgaaac ctcgcaaagt cattgaagcg atgaaagttt
     8821 tctctgatcc acgctttaac attgatgttc ttaaagtaga agtaccagtt aacgttaaat
     8881 atgtcgaagg ttttgctgat ggtgaagtag tttacagtaa agctgaagct gctgacttct
     8941 ttaaagcaca agaagaagcg actaaccttc catacatcta cctaagtgca ggtgtatctg
     9001 ctaaattgtt ccaagaaaca ttgcaatttg ctcacgactc aggtgccaag tttaatggtg
     9061 tgctttgtgg acgtgctact tgggcaggat ctgtagaacc ttacatcaaa gaaggtgaaa
     9121 aagcagcacg tgaatggttg cgtactactg gattcgaaaa tattgatgaa cttaacaaag
     9181 ttcttgttaa aacagctagt ccatggactg ataaagtata gtaaaaacat aaaacggagg
     9241 atattgttat gaacagagaa gagatgactc tcttagggtt tgaaattgtt gcttatgctg
     9301 gagatgctcg ctctaaactt ttagaagcgc ttaaagcggc tgaaaatggt gatttcgcta
     9361 aggcagatag tcttgtagta gaagcaggaa gctgtattgc agaggctcac aattctcaga
     9421 cagctatgtt ggctcgagaa gcttctgggg aggaacttcc atacagcgtt accatgatgc
     9481 atggtcagga ccacttaatg actactgtct tattaaaaga tgtgattcat cacctcatcg
     9541 aactttataa aagaggagca aaataatgca taaactcatt gaacttattg agaaagggaa
     9601 accgttcttt gagaaaatct ctcgaaacat ctatcttcgt gctattcgtg atggatttat
     9661 cgctggtatg ccagtcatcc ttttctcatc tatctttatc cttattgcct atgtcccaaa
     9721 tgcttgggga ttccactggt caaaagacat tgagaccttc ttgatgactc cttatagcta
     9781 ttcgatgggt atcctagcat tctttgttgg tggtactacc gctaaagctt tgacggactc
     9841 aatgaaccgt gacctacctg cgaccaatca gattaacttc ttatctacga tgctagcgtc
     9901 tatggtaggg ttccttttga tggcagctga gcctgcaaaa gaaggaggct tcttgacagc
     9961 cttcacggga acaaaaggtc ttttgacagc atttattgca gcctttgtga cagttaatgt
    10021 ttacaaggtc tgtgtgaaaa ataatgttac tattcgtatg cctgaagagg ttccaccaaa
    10081 tatctctcaa gtatttaaag acttgattcc attcactgta gcggttgttc tcctttatgg
    10141 atttgaactc attgttaagg gaactcttgg tgtaaccgtt gcagaatcaa tcggtaccct
    10201 tcttgctcct ctcttctcag ctgcagatgg ttaccttgga attacactta tctttggtgc
    10261 ttatgccttc ttctggtttg ttggtatcca cggtccttca attgtcgaac cagcaatcgc
    10321 tgctatcact tatgccaata tcgatgccaa cttgcatctt atccaagctg gacaacatgc
    10381 agacaaagtt atcacttctg gtactcaaat gtttattgtt accatgggtg gaacaggagc
    10441 tacattgatt gttccattct tgttcatgtg gatttgtaaa tcagaacgta accgtgccat
    10501 cggacgtgcc tcagttgttc caacattctt tggggttaat gagccaatct tatttggtgc
    10561 accaatcgta ttgaatccga ttttctttgt accgttcatt ttcgctccga ttgtcaacgt
    10621 ttggatcttt aaattctttg ttgacacatt gaacatgaac tcattctctg ccaaccttcc
    10681 atgggttact cctggtccgt tgggaattgt actcggtact aacttccaag ttctatcatt
    10741 tatcttagcc ggactcttgg tagttgttga tactatcatt tattacccat ttgttaaggt
    10801 atacgatgaa caaattcttg aagaagaacg ttctggtaaa acaaacgatg ctcttaaaga
    10861 aaaagtagct gcaaacttca atactgctaa agcagatgcc gttcttggaa aagcaggcgt
    10921 tgctaaagaa gatgtagcag caaacaataa tatcacaaaa gaaactaatg tccttgttct
    10981 ttgtgcaggt ggaggtacaa gtggcttgct tgccaatgcc ttgaacaaag cagcagcaga
    11041 atacaatgtt ccagtcaaag cggcagcggg tagttacggt gctcaccgtg aaatgttgcc
    11101 agagtttgac ttggttatct tggctccaca ggtcgcttca aacttcgatg atatgaaggc
    11161 tgaaacggat aaattgggca ttaaacttgc aaaaactgaa ggagctcaat atatcaaatt
    11221 gacacgtgat ggacaaggtg ctcttgcatt tgttcaacaa cagtttgatt aataaatttg
    11281 taaaagttat ttgagacgag attattcttt caattcctta taatattgtt aaccatatca
    11341 ttgcatttca tttccttggt ttccggaact cagaatatcg tctcaaatgc tttttttgaa
    11401 aggatttata gttatgacta aaacacttcc taaagatttt atttttggtg gtgctacagc
    11461 tgcttatcaa gcagaaggtg cgactcacac ggatggtaaa ggcccagttg catgggataa
    11521 gtatcttgaa gacaattatt ggtacacagc agagcctgca agtgattttt accataaata
    11581 tcctgttgat ttagaattag ctgaaaaata tggcgttaat ggtattcgta tttcaattgc
    11641 ctggtctcgt attttcccaa ctggctatgg cgaagtaaat gaaaaaggtg ttgaattcta
    11701 ccataatctt tttgccgagt gccacaaacg tcatgttgag ccattcgtga ctttgcacca
    11761 ctttgacaca ccagaagttc ttcactcaga tggagatttc ttaaaccgcg aaaatataga
    11821 acactttgta gattatgccg ctttctgttt tgaagagttc ccagaagtaa actactggac
    11881 aactttcaat gaaattggcc caattggaga tggtcaatac ttggttggta aattccctcc
    11941 aggcattcaa tatgatcttg ccaaagtctt ccaatcacac cacaatatga tggtttctca
    12001 tgcacgtgct gtgaaattgt ataaagataa aggttataaa ggcgaaattg gtgtagtaca
    12061 tgcgttgcca accaaatacc cttatgatcc agaaaatcca gcagatgttc gtgccgctga
    12121 gttagaagat atcatccata acaaatttat cttggatgca acctaccttg gacactattc
    12181 agataaaacg atggaagggg tcaaccacat cctagctgaa aatggtggag aacttgatct
    12241 tcgtgatgaa gacttccaag cacttgaagc agctaaagat ttgaacgatt tccttggtat
    12301 caactactac atgagtgatt ggatgcaagc ctttgatggt gagactgaaa tcattcacaa
    12361 tggtaagggt gaaaaaggaa gttctaagta ccagattaaa ggtgttggtc gccgaatcgc
    12421 accagattat gtgcctagaa cggattggga ctggattatt tatcctgaag gcttgtatga
    12481 ccaaatcatg cgagtgaaaa atgattatcc aaactacaag aagatttaca tcactgaaaa
    12541 cggtcttgga tacaaagatg agtttgtaga taataccgtt tatgatgatg gtcgtattga
    12601 ttacgtgaag aaacacttgg aagttttatc agacgcaatt gcggatggtg caaatgttaa
    12661 aggttacttc atctggtcac ttatggatgt cttctcatgg tcaaacggat acgaaaaacg
    12721 ttatggacta ttctatgtag actttgacac gcaagaacgc tatcctaaga aatcagctca
    12781 ttggtataag aaagtagcag aaactcaagt gatagagtaa tattacttga aaatgtaaaa
    12841 aattgttatc cgatacttta gaaaaagtac aacacttatt tagaccaggc tttgaaaagg
    12901 tatatattgt tagtttcgaa gattgtccta tgattcctga gcttgaagct accccgttgc
    12961 taaaatgtgg taaatggtat gtttcaactg gaaaagagtg gatttgccat tctgatttag
    13021 agttatcagc atttgaatgg gaatttttac aatcactcga tgtagaaata cgtgagacta
    13081 ttcacttcga ggtaaattat ttgcctttcc aataataaaa agaaaggaat tagttgtgac
    13141 tatcgaatta aaaaacgagt atcttactgt tcagtttaaa acacttggtg gtcaactgac
    13201 ttcgattaaa gacaaggatg ggatagagta tctctggcaa gctgatccaa attattggaa
    13261 tggacaggcg cctattctat ttcctatctg tggtagttta cgaaatgatt gggctattta
    13321 tagacctcaa gaaagaccct tttttacagg tcttattcgt agacatggtt ttgttcgaaa
    13381 agaagaattt attctggaag aagtcaatga taatagcgtg acctttagta ttaaaccaaa
    13441 tgctgagatg cttgataatt acctatatca gtttgaactg agagttgttt ataccttgaa
    13501 tggtaaatca attagaaccg aatttcaggt aacgaacttg gaaatggaaa aaacaatgcc
    13561 ttactttatt ggagcccatc cagcattcaa tagtccttta gtagaaggtg aaaactatga
    13621 ggattattat attgaattta gtgaggttga gtcttgctcg ataccaaaga gttttcctga
    13681 gacaggatta ttagatttgc aagatcggac accttttttg gaaaatcaaa aaattctcga
    13741 tttggactac tcactttttt cccatgatgc cattacactt gaccagctca agtctcgtag
    13801 cgtaacgctt cggtcaagga agtctggtaa aggtcttcga gtagattttg atgattttcc
    13861 gaacttgatt ctgtggtcta caactaataa aagtccattc attgctttgg aaccttggag
    13921 tggtctctcc acttcacttg aagaaggtaa tttcttagaa gataaaagac aggtaacgaa
    13981 aatttcacct caagaaactt ctagaaaaat ctatgatatt actatcctta attaaaattt
    14041 gttattattt gagtcttcaa taagatttga atactcttac cttgtgatga tagattggtc
    14101 ttctccgtgc cagtcttaca tcgcaaggtt tttaagtttt ttgtgggtct atgtcaactg
    14161 tagtgggtga cgaaaagcta atatctaaag acagtctggt agtaaaaaat aagattattt
    14221 actacttctt gatttaaaat aatagtggag ccattaattt gtggcaaatt taatttaata
    14281 ttttgcctca aattagccac actttttata gaactaagag gaaaaatgaa attttcttcc
    14341 tattaatata gttgtttgaa atcttaaata tagcg
//
LOCUS       DAFFFQ010000076        13917 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000076
VERSION     DAFFFQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.076"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            131..505
                     /locus_tag="LQB69_002301"
     CDS             131..505
                     /locus_tag="LQB69_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3526442.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LQB69_002302"
     CDS             complement(619..1248)
                     /locus_tag="LQB69_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL3526443.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LQB69_002303"
     tRNA            complement(1382..1454)
                     /locus_tag="LQB69_002303"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LQB69_002304"
     tRNA            1668..1741
                     /locus_tag="LQB69_002304"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LQB69_002305"
     CDS             complement(1879..2043)
                     /locus_tag="LQB69_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526444.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LQB69_002306"
     CDS             2338..3666
                     /locus_tag="LQB69_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL3526445.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LQB69_002307"
     CDS             3868..4701
                     /locus_tag="LQB69_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL3526446.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LQB69_002308"
     CDS             4717..5454
                     /locus_tag="LQB69_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL3526447.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LQB69_002309"
     CDS             complement(5609..6577)
                     /locus_tag="LQB69_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL3526448.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LQB69_002310"
     CDS             6809..7642
                     /locus_tag="LQB69_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL3526449.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LQB69_002311"
     CDS             7676..8515
                     /locus_tag="LQB69_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3526450.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LQB69_002312"
     CDS             8541..9101
                     /locus_tag="LQB69_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL3526451.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LQB69_002313"
     CDS             9300..11021
                     /locus_tag="LQB69_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL3526452.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LQB69_002314"
     CDS             11186..12328
                     /locus_tag="LQB69_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL3526453.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LQB69_002315"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LQB69_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL3526454.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAFFFQ010000077        13808 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000077
VERSION     DAFFFQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.077"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LQB69_002316"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="LQB69_002316"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL3526455.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="LQB69_002317"
     CDS             complement(2261..2512)
                     /locus_tag="LQB69_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL3526456.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="LQB69_002318"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="LQB69_002318"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL3526457.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="LQB69_002319"
     CDS             3462..3680
                     /locus_tag="LQB69_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526458.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="LQB69_002320"
     CDS             complement(3969..4481)
                     /locus_tag="LQB69_002320"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL3526459.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="LQB69_002321"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="LQB69_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL3526460.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="LQB69_002322"
     CDS             complement(6934..7377)
                     /locus_tag="LQB69_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL3526461.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="LQB69_002323"
     CDS             complement(7378..8166)
                     /locus_tag="LQB69_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526462.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="LQB69_002324"
     CDS             complement(8163..9104)
                     /locus_tag="LQB69_002324"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL3526463.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="LQB69_002325"
     CDS             complement(9281..9868)
                     /locus_tag="LQB69_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL3526464.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="LQB69_002326"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="LQB69_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL3526465.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="LQB69_002327"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="LQB69_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL3526466.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="LQB69_002328"
     CDS             complement(12785..13720)
                     /locus_tag="LQB69_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL3526467.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAFFFQ010000078        13486 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000078
VERSION     DAFFFQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.078"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(163..387)
                     /locus_tag="LQB69_002329"
     CDS             complement(163..387)
                     /locus_tag="LQB69_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL3526468.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(585..2165)
                     /locus_tag="LQB69_002330"
     CDS             complement(585..2165)
                     /locus_tag="LQB69_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL3526469.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLYQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2566..3942)
                     /locus_tag="LQB69_002331"
     CDS             complement(2566..3942)
                     /locus_tag="LQB69_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL3526470.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4070..5188)
                     /locus_tag="LQB69_002332"
     CDS             complement(4070..5188)
                     /locus_tag="LQB69_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL3526471.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5330..5764
                     /locus_tag="LQB69_002333"
     CDS             5330..5764
                     /locus_tag="LQB69_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3526472.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5842..6375)
                     /locus_tag="LQB69_002334"
     CDS             complement(5842..6375)
                     /locus_tag="LQB69_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL3526473.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6469..7647)
                     /gene="mutY"
                     /locus_tag="LQB69_002335"
     CDS             complement(6469..7647)
                     /gene="mutY"
                     /locus_tag="LQB69_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL3526474.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7640..8437)
                     /gene="recX"
                     /locus_tag="LQB69_002336"
     CDS             complement(7640..8437)
                     /gene="recX"
                     /locus_tag="LQB69_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL3526475.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8544..9926
                     /gene="rlmD"
                     /locus_tag="LQB69_002337"
     CDS             8544..9926
                     /gene="rlmD"
                     /locus_tag="LQB69_002337"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL3526476.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10100..10678)
                     /locus_tag="LQB69_002338"
     CDS             complement(10100..10678)
                     /locus_tag="LQB69_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL3526477.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11000..11470
                     /locus_tag="LQB69_002339"
     CDS             11000..11470
                     /locus_tag="LQB69_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL3526478.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11578..12804)
                     /locus_tag="LQB69_002340"
     CDS             complement(11578..12804)
                     /locus_tag="LQB69_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL3526479.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         3957 a         2719 c         2248 g         4562 t
ORIGIN      
        1 aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag aagcaactcc
       61 ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa ttccagctta
      121 tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt ttgatttttc
      181 tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt tatttttaac
      241 tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt ttttgtccca
      301 gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct gtttttttct
      361 ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa tcatatgtac
      421 gcaaaataat ttttatattt ttttgattag acatttctat gattttaata tagtaaaaag
      481 agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt ttttgtttgg
      541 ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat caaacttttt
      601 aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt ctcgaataag
      661 aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact catcttctca
      721 tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga catgattact
      781 tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag ctgaccaaca
      841 gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc tccttcttga
      901 actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt tgcagtcact
      961 ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt tagttttcct
     1021 tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat gatatcgcct
     1081 gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac attgcttaat
     1141 ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa tgttccagaa
     1201 cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc ttggatcttg
     1261 aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc ttgtgttttg
     1321 tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg tacaccgaag
     1381 ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat tttttcttga
     1441 ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg ttcatataag
     1501 gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa ttctactaat
     1561 cgttcatcct gataattttc cggacgataa aactctacac gattatggta gatatcataa
     1621 agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga ttcgatatct
     1681 aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg atctagtttg
     1741 ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggtataa tttctttgtc
     1801 tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac ggccatcaaa
     1861 gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat atttactcgt
     1921 ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg ttgtttctcg
     1981 atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac ggtcccggct
     2041 tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt cccagcatcc
     2101 ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa ttttggatta
     2161 ttcataacct aactccattt cttccattca attatttata ttttctctag gcacagattg
     2221 tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg tgtagcagtc
     2281 tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc cacccaagcc
     2341 aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag gatagctttt
     2401 tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag cgaatgaaaa
     2461 agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga aaagacgtgt
     2521 tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc ttcttggctc
     2581 gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg cttctgtttc
     2641 ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca tttcttcaga
     2701 aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg gaatcatgcc
     2761 taatgctgta atcaaataac cagccatcgt atctacatca ctcatatgca gatcactgtc
     2821 aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt cgtgttcatc
     2881 gattttttca taaagattct caacttcatc tgattcatca tcgatttctc cgacgatttc
     2941 ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat ctaataaaat
     3001 agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa agaccgtttc
     3061 aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa agccgaattt
     3121 atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat ctttgtcttc
     3181 gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat tttcttccac
     3241 agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt ctcgggcaac
     3301 tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat tcaatacgcc
     3361 ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt catcatcaaa
     3421 agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc agacgaaggg
     3481 cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga cttcttctga
     3541 tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata cataggtcaa
     3601 aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac ctaataccgg
     3661 agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt tgaccaaagt
     3721 gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt ttaaaagctt
     3781 ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat tgatcgaaac
     3841 aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga tcaataataa
     3901 aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat tctctcctta
     3961 taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac gttaaaatgg
     4021 tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc tacgaggtcg
     4081 tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg attatatagg
     4141 aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca agcaatccgg
     4201 caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt ttcagtgatt
     4261 tcccaattac attaggataa atgatattcc catccaactg ttggacgatt agcacaacca
     4321 cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat gcaggtgcta
     4381 gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg accccaaata
     4441 agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta ccaacgaata
     4501 aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt tgccccaata
     4561 actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt ggcactagtt
     4621 tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt actacggttg
     4681 ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta aggaattgct
     4741 gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata tctacttcct
     4801 tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg atgaaatcag
     4861 gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca ctaagtaaaa
     4921 tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat attcttttga
     4981 tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga aagcctgcaa
     5041 tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa ataaagttta
     5101 tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac caaaacatta
     5161 gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt actagtatat
     5221 attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg ccatactttt
     5281 tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca tgaaagttgt
     5341 acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca ggcatcaagt
     5401 ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg aagcccattg
     5461 tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac gtcttttgcc
     5521 tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat acagacacca
     5581 agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc aaggctataa
     5641 tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac taggatatac
     5701 agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga aaaagacttt
     5761 ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa tctctcactg
     5821 gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt tataccaaat
     5881 atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa ccagaatctt
     5941 cacattttct tttaggatga agtcaatatc gttcgggtaa ttcatcattt tgctgtgcat
     6001 ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct tgatatccaa
     6061 gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag ctagattaca
     6121 ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt attttttatg
     6181 aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt cagtgactaa
     6241 tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca tggtatctcg
     6301 ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa actctccttc
     6361 ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt gtttgggtgt
     6421 tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct agaaaggatt
     6481 cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct taggaaaaac
     6541 gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg taaattcttg
     6601 ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca gatgactaaa
     6661 aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct gcttttcaaa
     6721 tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt ctggctttgt
     6781 ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca ttggaaatgt
     6841 ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg ctccagaatg
     6901 attttgaaga gctgcactga tataataaac gtcttttggc tttgcttttt ttgtttttac
     6961 aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga tcggacaagt
     7021 ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca ttgcttggtt
     7081 gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa agattttcct
     7141 gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta ctcgcatcac
     7201 gttgccgtca actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg cgcctgtcgt
     7261 atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca tttccccatc
     7321 aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg agtaataacc
     7381 aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct cttcaattgt
     7441 aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc tggtttgctg
     7501 aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc aaggcaggtt
     7561 tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg tttctttatc
     7621 tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg aatcgctgga
     7681 tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga tttctttttt
     7741 taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact aaataatcat
     7801 attctatttc ttgatctgct tcttgcggaa gatcagctat cgcttgttgg atcacttctt
     7861 gagaaaagcc tttcgtcatc aatgtttgtt ggatttttct caataattct ttttggcttt
     7921 ttccgtgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga acttggtctt
     7981 caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct ttttgctgca
     8041 tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg cgcacataac
     8101 tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct acgattttat
     8161 ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct ttttccgatc
     8221 gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct ccgctactct
     8281 tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat cgatagcgta
     8341 ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg cgatagaaag
     8401 ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc gaacatatat
     8461 tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa taaaccacag
     8521 aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga caaacggttc
     8581 ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc aaacggttga
     8641 tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc acgaaagcaa
     8701 ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa gatcgcgtag
     8761 tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac ttagcttatc
     8821 aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa ttcaaaccag
     8881 caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct tggcattata
     8941 gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt gaagctggtt
     9001 tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt caagaacctc
     9061 gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat gcacccatct
     9121 atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc ggtgttaaaa
     9181 ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa aaaaacgcaa
     9241 tgatccgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa aatgtccagg
     9301 cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga aaagaagcca
     9361 ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc tttcaactca
     9421 atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat gtccagccta
     9481 atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt gtagcaaaac
     9541 aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat gcaaaaaaaa
     9601 atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca gagcaagttc
     9661 tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat ccaccacgta
     9721 cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa aaacttgttt
     9781 atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt aaaaagttca
     9841 agattgaata tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt gaagttgtcg
     9901 tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct gccctgtatc
     9961 aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac tgtacttgtg
    10021 accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac atagtttttg
    10081 tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa aatgcatttg
    10141 tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga tcctgcggaa
    10201 gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct atttttgtga
    10261 tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt ttggtaatgt
    10321 agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata tttaatgttc
    10381 ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa ggctcaaagc
    10441 cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta gcttcgatac
    10501 tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct gctgatgttc
    10561 ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct ggaatgctaa
    10621 aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc tttttcatgt
    10681 agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa cacaaacatt
    10741 atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg cagattataa
    10801 cagaagtaga cgagaaaagt acaaatagaa aacatgagaa tcaaatcata tgatcttttt
    10861 caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat tatcaatttt
    10921 tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta aactaatatt
    10981 atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg tttgtgttat
    11041 aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg gaaaatatta
    11101 ctattctttg caacttccac catttgcacc acctagctgg atatttgggc cgatgtggac
    11161 cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca acaacaaaaa
    11221 acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt ttatatggtc
    11281 tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta cacttttagt
    11341 tattttcttg tcagtgctta tgtatcagtt gtggctgcat catcgcctgg caatgtggct
    11401 catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt caatttattt
    11461 tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa agtgccacct
    11521 aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta tttttttcta
    11581 gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat tttgttcctg
    11641 tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact gacttgtatc
    11701 ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt tttctgaata
    11761 ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg aatcgccatc
    11821 ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag gtaatgtatc
    11881 gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa gatcattttt
    11941 tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca actggtcttg
    12001 tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt catcccaatc
    12061 taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat tgcttacgcc
    12121 atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac gacgttgtcg
    12181 accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac ctttttctcc
    12241 attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt caattttatt
    12301 attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca aattgatcgt
    12361 tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg ccatttcgac
    12421 accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta cgatattcgt
    12481 ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat taactgttac
    12541 taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg tatctaaacc
    12601 taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag aacgtttgga
    12661 ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt ctcccataaa
    12721 tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta acaaggttaa
    12781 tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta aaatattgtc
    12841 tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt taaaaaacat
    12901 ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat cataccatac
    12961 cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc tttttttatg
    13021 tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc agtaaaatga
    13081 tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat tatcattcga
    13141 tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc tattcgtttt
    13201 atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca ttaccatcat
    13261 cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta aagtaataga
    13321 attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt attcaacatc
    13381 caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct tgcttgataa
    13441 ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaa
//
LOCUS       DAFFFQ010000079        13427 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.079, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000079
VERSION     DAFFFQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.079"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..912)
                     /locus_tag="LQB69_002341"
     CDS             complement(<1..912)
                     /locus_tag="LQB69_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL3526480.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1340..1903
                     /gene="efp"
                     /locus_tag="LQB69_002342"
     CDS             1340..1903
                     /gene="efp"
                     /locus_tag="LQB69_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL3526481.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2004..2174
                     /locus_tag="LQB69_002343"
     CDS             2004..2174
                     /locus_tag="LQB69_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526482.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2389..3648
                     /gene="lysA"
                     /locus_tag="LQB69_002344"
     CDS             2389..3648
                     /gene="lysA"
                     /locus_tag="LQB69_002344"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL3526483.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3635..4435
                     /gene="dapF"
                     /locus_tag="LQB69_002345"
     CDS             3635..4435
                     /gene="dapF"
                     /locus_tag="LQB69_002345"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL3526484.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4438..5319
                     /locus_tag="LQB69_002346"
     CDS             4438..5319
                     /locus_tag="LQB69_002346"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL3526485.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5316..6032
                     /locus_tag="LQB69_002347"
     CDS             5316..6032
                     /locus_tag="LQB69_002347"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL3526486.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6038..6733
                     /gene="dapD"
                     /locus_tag="LQB69_002348"
     CDS             6038..6733
                     /gene="dapD"
                     /locus_tag="LQB69_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL3526487.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6749..7750
                     /locus_tag="LQB69_002349"
     CDS             6749..7750
                     /locus_tag="LQB69_002349"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL3526488.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEKPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7747..9108
                     /locus_tag="LQB69_002350"
     CDS             7747..9108
                     /locus_tag="LQB69_002350"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL3526489.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9068..10219
                     /locus_tag="LQB69_002351"
     CDS             9068..10219
                     /locus_tag="LQB69_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL3526490.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10194..11363
                     /locus_tag="LQB69_002352"
     CDS             10194..11363
                     /locus_tag="LQB69_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3526491.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11538..12917
                     /locus_tag="LQB69_002353"
     CDS             11538..12917
                     /locus_tag="LQB69_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL3526492.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13198..13427
                     /locus_tag="LQB69_002354"
                     /pseudo
     CDS             13198..13427
                     /locus_tag="LQB69_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4483 a         2339 c         2923 g         3682 t
ORIGIN      
        1 gtaaatgttt tttgataaat agcggtctgc tgcgtctgga aaaatcgtaa cgacacgatg
       61 accaggtggt aaacgacgaa tttctcttaa tgctgctgca aaagccgcac cactcgaact
      121 tccaactaat agtccttgtt ctcgtgctaa ctgcctagta tagttaaacc cttctacatc
      181 agagattgtc tcgatctgat tgattgaaag gggagaaaga aacggcggaa tgaattctac
      241 accgataccc tcaatttcgt gaggggctgg atcaccacca tttaacacag agccctctgg
      301 ttctacaccg attattcgaa tgtcgggata tttttctttc aaaaaggtag aagttccagc
      361 aaaggttcca ccactcccaa ttcctgctac aaaactatgg atattttctt tgatttcatg
      421 aaagatttct ggccctaacg tatgatagta agcagcagga ttatctttat tttcaaattg
      481 aagaggcaga tagctgtttg atatctcctc tgcaagtttt ttacttttgc tgatggcacc
      541 gatgattcct tgctcactag gagaatggat gatctttgct cccaatgctt tcatcaattg
      601 ttgtttttct aagctaaact tctctggaac aacaaaaacc gttcttaatt tgtatgtgag
      661 tgcggccaga gcgatcccaa ttcctgtatt tccggcagtt ggttcgatga tcgtcgtttt
      721 ttcattgatt tttccttggg caaatccttc tttgatcaaa taactgccca atctatcttt
      781 gatacttcct ccaggattta aatattctag ttttgcataa atagcagaac ctttaggcat
      841 agaatagtgc tgattgtcaa agcgataaag cggtgtggaa ccaatagctt ctgtaattgt
      901 cgagataatc atgtgggcct cctaaaaaaa gataataaaa aaaggagctg actattaaat
      961 cagctccttg aaagacatag agaaaaattg agtagataaa aagataagtt acctggtact
     1021 cattctatga agggcaatta atagacatga caaatagtat gctgctttta tgcaccatac
     1081 aacagcaacg attcaattgt ttttggttgc tgactgtcat gtgtatccct ccaagtaaaa
     1141 tttttattaa cgatagcatg aaaaaaaata aaagtcaaag gcttgaaaga aaaaaatatt
     1201 tcgatagcat cgtttacccg tgtttatttt tctgacaaat aggcatgaga ccttaaaaga
     1261 tagagaatac tctagttttc tggccggttg tctgatacaa taaagttgat tatgataaag
     1321 aaaagaagga gcaaaaataa tgatctcagc aagtgattta agagccggta tgactttcgt
     1381 acaagacggc aaactgatta aagttctgga tgcaagccac cataaacctg gtaaaggaaa
     1441 tacagttatg cgtatgaaac tgaaagatgt ccgttcaggt gcaacatacg acacaacgtt
     1501 cagaccagaa gaaaaatttg aaaaagcaca tatcgacaca aaacctgtac aatatttgta
     1561 cacgatggat gatacagctt tcttcatgga tttggaaaca tatgagcaat atgaaattcc
     1621 tgtagaaaca gtagaagaag aaatgaaata tatcctagaa aacatggaag taaaaatcca
     1681 atttttcggt agcgaagtca tcggcgttca attaccaaca actgtcgtat tacgtgttgt
     1741 ggaaacacaa ccttccatca aaggtgcaac agtcactggt tcaggtaagc cagcgacaat
     1801 ggaaactggc ttagtagtca atgttcctga cttcatcgaa acagatgaat tgttggaaat
     1861 caatacagca gaaggttctt atgtaaaacg tgcgtcaaaa taattcgcta acggattgat
     1921 aagaatttga aaacagagct tgacctcttg aaaggcagat catcggatct gcctttcaag
     1981 aggttttttg tggaatcagc aaattgaaaa ataaaaaagt atttgatttt atttatattt
     2041 tgaaagatga atggcaaaag ctagagaaag aaaaaactgc acttatttat gaagaaattg
     2101 aaatgattca taaaattgaa aatatccgaa ctattccgaa ctatattgaa gcgaaaatat
     2161 tttttcatca ctaaatagtg ttgaatttat gtttttatgt ataaatagct tctaatagtt
     2221 cgggtatttt gtttttataa ccatttttat tcttattttt aaaaaaatat ttcgaaatat
     2281 actagacaat tcagaaaatt ttgtttaata ttattccaat aaaattcaaa tctttatcaa
     2341 atgaaattcc aataaaaacg gaaaaaaagt gaaaagagga atgaaaaaat gacagaattg
     2401 ataattggcg gagtcacagc aagcacattg gcagaaaagt ataagacacc tctgtatgtg
     2461 tatgatgaag aaaagatgag acaaacgatg ctgtcattca agcgaggctt tgtatcgaaa
     2521 cgttttgata caaaagtgct ttacgcctct aaagcattcc aaacgattga gatgctgcat
     2581 ttagttcgtg aatatggatt cgggctagat gttgtcagtg gtggggaaat ctataccgct
     2641 ttgcaagctg attttcctag agatactatc tacttccatg gtaacaacaa gacgccagag
     2701 gaactgatct atgcacttga aaatgatttg ttgcatattg ttgccgataa tttgatggaa
     2761 gttgaattat tagcagaatt aagccaacgc tatttaaaaa aaatctgtat catgcttcgg
     2821 ttgaatgtcg gagtagaagc acatactcat gaatatatcg tcactgcgca tattgattca
     2881 aaattcggca tgagctatga aagtaaagag tgccaacaag cccttcagtt gataaaagaa
     2941 agcagctatc tagagttaga aggatttcat gctcatatcg ggtcacaaat ttttgatgtt
     3001 actgcttggt tagctgaaat cgacaaattg acgaattact taaccgattt tgaccaacct
     3061 ctttccttga atcttggcgg agggtttggg attcgttata cacaaaatga tcagccgatc
     3121 ccagtcgaag aggcactaaa aaaactgatt gaatatacgg aagaagcttt ggaaaaacga
     3181 aaactgacca ttcgtcaatt actgatcgaa ccgggtagaa gtattgttgg agaagctggt
     3241 acaacactat acacgattgg tttcatcaaa aagacaccac ataaaaaata ttactttgta
     3301 gatggcggga tgacagataa tatccgtcca gctctgtatc aggcagaata cgattgtgat
     3361 ttagcagcaa aactgtcgga agaaaaaaca gagaaagtga cgattgcagg aaaaatgtgt
     3421 gaatcaggcg atgtggtaat taaagaagct tatttgccga aagcagaatc gggtgatctt
     3481 ttagctgttt attccacggg agcttatgga tactcgatga gcagcaacta caatcgagcg
     3541 acacgaccag cagttgtttt tgtcaataat ggccaatcaa gactgattgt tcgcagacaa
     3601 gcattttctg atctactaag gggggaagtt tcctatgatc cttcataaat ataatggctg
     3661 cggaaatgat tttatcttat tagattacgc agaagacaca gattatagtc aattagccgc
     3721 ttctttatgc acaaaagaaa aatatgatat agacggattg atcgccgtaa aaatcgatcc
     3781 gttggaaatg atctattaca acaaagatgg cagccgcgcg ccgatgtgcg gaaatggcat
     3841 tcgctgtttt gctcgatacg taaatgatca gaacatgatg aatgaacagg aattcaacgt
     3901 agagacatta gcaggcacaa gaaaaattac ggtgttggat ccctcacctt tttattgcag
     3961 cgtagatatg ggagtgccag atgattctcc ggaaaaaatc aaagctaatc aaacttatcc
     4021 aataaaaaac caggaaatag agatcaatgg tgaaaaagta acgatcacta gtttattttt
     4081 aggaacgatt cataccgttg tttttgttga aaatgcgcgt gaggaagtct ggagaaaaag
     4141 aggtgaactg atctgtcatc atcctttgtt caaagaaaaa accaatgtca actttgtaga
     4201 agtagtcaat gaacaagaat tgatcgtccg tacctatgaa agaggagtag gttggacact
     4261 tgcttgcggt acaggttgct gtgcctctta tgtggttgct aaggaaactg ggaaaatttc
     4321 agcagatcag gtagttgttc acttggaaca aggagacttg atgatctctg gtgaggatgc
     4381 gatacaaatg gctggcccag cagttcatga atggacaaga atattggagg aatgaagatg
     4441 ataaaaggtt cgatcgtggc gctgatcacg ccgatgaatg aagagggatc agtagattat
     4501 gctggtttgg aaaaattgat ccaattccat ttagatgaac aaacagatgg attattagtt
     4561 ttaggtacaa caggagaatc ttctacgctc acacaatctg aggaggagca gatccttcaa
     4621 ctgacagtaa agaaagtggc tggccgtgtg ccggtgattg caggagctgg aacgaataat
     4681 acgaaagaaa cgattgaaaa agcgaagcat tttgcttctt tgggggcaga cgcacttttg
     4741 gtcatcaccc cttactacaa caaaacaagt gatgcaggac tagctgctca ttttacagca
     4801 attgcagaag cctcaccgat tccgcttatt ttgtataatg ttccttctag aacaggcatg
     4861 tcgattccaa tccatgtatt agtaaattta gcagaacatc ctaatatcat tggtttgaaa
     4921 gaagcctcag gagatatggc ttatgtgatg gatgccgctc ggttgattgg agaagaattc
     4981 ttcctttaca gtggaaatga tgatttgatc ctaccagtaa tgagtgtagg aggaagcggt
     5041 gtaataagtg tatgggcgaa tatccaacct aaaatagtcc atgaattagt aaaagatacg
     5101 caggacggaa gatggcagca agcaaaagaa aagcaattaa atgcacttga actgatccat
     5161 gccttgttca gtgagaccaa tcctattcct gtcaaagcag ccatgtcatt attagactta
     5221 ccttccggac ctctacgcct gcctttagtt tctctttcaa aagagaaaaa gaaacaatta
     5281 gctcagcttt tgttaaaaga aaggacggtt gaaaaatgaa aatcggactg attggcagtg
     5341 gacagatggg aaatagagtc gaagaagttg cacaagaaag aggagatacg gtccttcttt
     5401 tacggacggc acctaaagaa gcaactacgc ttgcttttac ggaactgcca gaactgctta
     5461 ttgatttttc tcatccagat aatttagaga aaatcctttc gtatagctta tcatatcaac
     5521 ttccgttagt cattggcacg acaggttata cacaatcaca gttccaagaa ataaaagaac
     5581 atgccaaaca tgtacctgtg atgtatagtg ctaatttttc gttcggcatc atggtgatga
     5641 atcaactaat caagcaagca gctgcaatgt tgcaaggctg gcagatcgaa ctgattgaaa
     5701 aacatcatag tcgtaaaaaa gatgcaccat ctggtactgc aaatatgctg cttcaaacaa
     5761 tccaagaagt gcaaaagctg cagcccgtat acaactggca agggaaaaaa agagaagaaa
     5821 accaaattgg tgtacacagt attcgtgcag gttctttacc tggtgaacat gatgtattgt
     5881 ttgctgctac tgatgaaata tttacgatca aacatgaatc tttttctaat cgaatctttg
     5941 ctgcaggcgc agtagaagca gcggactggt tgaaagatca gtcaccaggt tattacaaat
     6001 tagaagattt attaatggac aaaggggtgt aaaaaacgtg gaagctcaag aaatcatccg
     6061 ctacattcaa acggcatcta aaaagacgcc tgtgaaagtc tatctgaatg taaccgagcc
     6121 aatcgaattt aaagaaagta aagtttttgg tgaaggaagc agcttcgttg tttttggtga
     6181 ctatgagacg attgctccgg ttttagaagc tgaatcagaa aaaatcattg aaatcgaaat
     6241 cgaaactgct gcaagataca gcgcagttcc tttgctagat attaaaaaaa ttaatgctcg
     6301 tatcgaacca ggtgctatta tcagagatca agtgtcaatc ggcaacaatg ccgtgatcat
     6361 gatgggagcg attatcaata ttggtgcagt aatcggtgaa aacacgatga ttgatatggg
     6421 tgctgtctta ggtggacgag cgacagtggg caaaaactgc catattggtg caggagctgt
     6481 cttagcaggt gtgatcgaac cagcatcggc caaaccggtt atcgtagaag acggcgtact
     6541 ggtaggtgcg aatgcagtga ttgttgaagg tgtccatatc ggaaaagatg cagtcgtggc
     6601 agcaggagca gtggttttag aagatgtggc agcagaaact gttgtcggtg gaatacctgc
     6661 acgtgtatta aaaataagtg atgatcagac aaaagaaaat acagcattga ttgctgcgtt
     6721 acgcgaatta tagaggagat ggagaaaaat gcgcgaaata gatacctgta ttgtgggggc
     6781 aaccggatta gtcggccaaa cgatgctcaa agtgttagaa gaatatgatt ttccagtagg
     6841 acgattgaaa ttattagctt caaaacgttc agccggtaaa gtttgtgtat tcaagaaaaa
     6901 gaaacacact gtacaggaat tgacagaaac ttcatttgaa ggatatgatt tagcgctatt
     6961 ttctgcaggt gcttctgtat ccaaagagtt tgcgccaatc gcacgaagtc agggactatt
     7021 tgtcatagat aattcctcag catggcggga agattcggat attccattaa tcgttccaga
     7081 ggtcaatctg gaagactata cattgaatcg gctgattgca aatccaaatt gttcaaccat
     7141 tcaagcggta cttccgctta aagctttaca agaagccttt ggtatccagc gagtcaatta
     7201 ttcgacctat caagcagttt ctggttccgg gcaaaaaggc attgaagatc ttcatcgtac
     7261 gcaaaatgac gagaaaccag ttttgtatcc ttataatatt agccaaacgg tgattccgga
     7321 aatcgatcag cctttggaaa atgggtatac aaaagaagaa cagaaaatga taaatgagac
     7381 ccgtaagatt ctgcatcagc ctgctttgcc agtatcagca acctgcgtac gtgtgccaat
     7441 cgaaaatgga catggagtct ctgtggcagt tcaactgcaa aaagagttta cgacggaaga
     7501 aattagagag tgtctcagcc agtttccagg gattcaatta gaagatgatc tagcagcaca
     7561 taaatatccg acgagcattc ttgctagagg aacagatatg gtttatgttg ggaggatcag
     7621 aaaagacact tctttagaga acggattgtt gttctatacg actgccgata acatacgcaa
     7681 aggagccgct gcgaatgccg tacagattgc tgcagcttta atgcagaaaa tagcggaggt
     7741 ggcaagatga gtaatacgct gaaagtagcg aaattcggcg gaacatcgat gtctcatgaa
     7801 gagcaattca aaaaagtccg tacaattgtt ctttcagatc ccaatcgcca agtgatcgtt
     7861 gtttctgctt taggcaaacg tacgtcagat gatgacaaag taacagactt actttatttg
     7921 atttatgctt accttcagca tcatgttgat tggacacccc tttggaaaag gatttgtgat
     7981 cgtttcattc aagtaagaga tgttcttgcc ctgcagttac ctattgaaga tgaactagca
     8041 cgcatacaga aacaactgga agacgaaaag ataactgaag attatttagt aagtagaggt
     8101 gaatatctga ctgctcaact gatggctgag tatcttggtt accagtttat tgacgcaaaa
     8161 gacctgattt tctttgacta tgatgggaaa atcgatcagg aaaaaacagc acaggaaatc
     8221 acaaaagcat ttaaaagata tccgaaaatg gttgttccag ggttttacgg ggcgaaccct
     8281 gcaggaaaag aaaaattatt gggccgtggg ggaagcgaca ttaccggcgc gatactggcg
     8341 aagatgttgc aagcggaaag ttatgagaat tggacggatg tttcaggaat catgatggct
     8401 gatccgcgaa tcatcgatca tcctaaaatc atcgaggaaa tctcttttag agaactgaga
     8461 gagatggctt atatgggggc taacgttctt catgaagcag ctgttttccc agttcaagag
     8521 gctgatatcc ccatccaaat aaaaaatacc aatgatcctt tcgctcaagg gacaaaaata
     8581 tcgaatcacg aaatagaaaa ggaaaatgga ctgacaggaa tagctggacg caaggatttt
     8641 ctttccatca ctttattcaa acgtcatatg tccgatgaaa tcgggtttat ctggaaagca
     8701 atgagcattt ttgctaaaca tgagatatca atcgaacata ttccttcagg gatcgacaat
     8761 atcggagtga tcgtctcagc agaagcgatt gccgataagt tatttctcat cacgaaagaa
     8821 ttaaaagaaa cacttggcgt ggaagaaatc gtcgtgatcg aagacttagc tttgatctct
     8881 gtcgttgggg gtccgcataa agaattgatt ggattatctg ggaaagtgtt atcgatattg
     8941 aacaaattag aaattcgcac atcgatctta tcacaaggag cacaggagtt gaatctgatc
     9001 atcggtgtac cgaataatca atatgaaaca gtagtgaagg gtatctatga agggatggtg
     9061 aatacggatg ctgaaagaat atcgaatggc actccatcaa attcctgaaa tcgggtttca
     9121 tgaattcaag acaaaaaaat atttatatga acaagtaaaa aacagcgagg ccgttatcca
     9181 tgaaatcggc gaaacaggac tactccttta ttttgataat cagcaagaag aaacgattgc
     9241 ttttcgaaca gatatcgatg cactgccagt caaagaagaa acgaatctct cttttgcttc
     9301 taagcatgaa ggttatatgc atgcatgtgg tcatgacgga catatggcga tgcttttagg
     9361 gttgactgac tttataacta aacacattga ggatcttatt tataatgtag tactgatcta
     9421 tcagccatca gaagaaattt ccggtggcgc acaaagcgtt attcagtcag gattgttgga
     9481 taaatatgaa gtacaagcaa tatttggctt tcatctatgg cctggactag ctaagggaga
     9541 agttttctct cgaccaggac ctttgatggc acaaagcagt gaaacagaca ttatcattaa
     9601 aggccagtct gcacatattg cttctagtga aaaaggaatc gatagtctgg aggcagctgt
     9661 acgatttttg aagaatgttt atgattttga ccggcaatta ccggcagagt ggaatcatct
     9721 aatgaaattt ggccaaatcc aaggtggaac gatccgaaat gttttagctg gagaagttat
     9781 tgtatccgga agtatccgtt cttacagttc agatatgcaa aattatttga agaggggatt
     9841 agaccattta gcagaacagt ttcactctac agctactatt tcttttcgct ataatgacgg
     9901 ctatccagct gtaatcaacc atagaggctt atatgcagca ctgttagatt caaacaaact
     9961 acatgaatta gatgctccag ttcttcaagc cgaagatttt ggcatatata cgcaacaata
    10021 tccatgtgta tttttctttt taggtgtcgg tgatacccct gctttacatg atccgacgtt
    10081 cgattttgat atggatgttt tagaaaatgg tgtagaatgg tacaaaacga ttctttttac
    10141 agaaaatctt cttgtaaaaa tcgatagcag ttcaaaagag gagcgagtaa ataatgatga
    10201 atttatccaa tcgatttagt cctaggcttt ctagaataga agtatcaaaa atacgacaat
    10261 tcgatcagca aatatcgtcg ataccagatg tgatcaaact caccctggga gaaccggatt
    10321 ttccaactcc tgaacatgtc aagcaagcag gaatagcagc gattgaagaa gacttttctc
    10381 actataccgg aatgagagga ttagaggaac tgcgggaagc ggcttgtata tttcagcaac
    10441 agcgttatgg attaacttat gatccgcaaa cggaagtttt gacgacagtt ggtgcaacgg
    10501 aagcgattgc ctctgcgttg ttatctgttt tggaagaagg agacaaggtt cttattccag
    10561 cgcctgctta ttcaggatat cagccattag tggaactagc tggtgctgaa ttgatcccga
    10621 tagatacttc tgacacaggg tttgtctgtc agccggaaca atttgaacga gcatttgaac
    10681 aatatggatc agctgtcaaa gcagtcattt taaactatcc taacaatcct actgggacga
    10741 cattgtctgc aaaacaattg aaagcaatcg ctgaggtttt aaaaaaatat ccggtatttg
    10801 ttataagtga tgaagtatat gctgaattga cttatagtgg aacgcatatg tccatagcta
    10861 cttatcttcc agaacaaaca atcgtgataa gcgggttatc caagtcacat gcgatgactg
    10921 gttggcgagt gggctttatt tttgctcaaa aagcccttat tgacgaacta attaaagtcc
    10981 atcaatatct ggtgactgcc gctacaacga tgtcgcaaaa agctgcggct gaagcgctaa
    11041 taaatggggt gaaagatagc gagaatatga aagagcaata tcaaacacga agagactatc
    11101 tggtccagca attggcgcct ttaggatttg aagtgaccca gccaaacggt gcattttact
    11161 tgttttgcaa actcccagct gccattcaaa ctgactcttg gcagttttgt gtagaattag
    11221 cggaacaggc aaaagttgcg tgtatcccag gaattgcatt tggcccagaa ggagaaggtt
    11281 atatccggat ttcttatgct agcagcatgg aaaaattaca tgaggcctgc aaacggatca
    11341 aagacttttt aacagaacag tgattcatag attttatcaa aaaaacaatc gttaattttg
    11401 cggtatattc tgcaaggaga cgattgtttt ttaaatttag ttcaaatgaa cgcgtttctt
    11461 ttgattcata actaatgtaa tgttaagttt aaattgttta gaatgatttt aaaagaatat
    11521 tcaaggaggt ttcatcaatg gaaaaaatca aagttgtcat tgtaggtgca tctcatggag
    11581 ggcatcaatc gatcttggaa ctattgacac gatacggtga aaatgtagat atcacgctat
    11641 ttgaagcagg agactatatt tccttcatgt catgtggtat ggagctgtat ttagaagaca
    11701 aggtaacaga tgtaaatgat gtacgtaatt tcagaccaga aaactttccg caatccaatg
    11761 ttcatatttt aaataatcac gaagtaaaaa cgatcgatgc cgataaaaaa acagtaacgg
    11821 ttactagcgc atcagataac gaaacaaaag aatatcctta tgacaaattg attttgagtt
    11881 ctggtgtcaa accaaacaat ctgccagtac caggagcaga tctagaaaat gtctacttga
    11941 tgcgtggata caattgggca accaaaatca aagaaaaatt aaatgatccg gctattaaaa
    12001 aaatcgctgt cgtaggtgcg ggttacattg ggatcgaagc agcagaagca agccgaaaag
    12061 ctgggaaaga agtcgtattg cttgatgtga tcgatagacc tttaggcact tatttagatg
    12121 ctgagatgac agatatttta gaacaacacc taaatgaaaa cggtattgaa gttttgacaa
    12181 atgcaaaaat cgaagctttt actggtaacg gaaaagttga ggcaatcaaa acaagtgaaa
    12241 aagaaatccc agcagatcta gtcattcaag ctgccggtgt gaaggcaaat acagaatggt
    12301 taaaagggat cgtcgactta gatgaacgtg gctggatcca aacaaatgaa tacttgcaaa
    12361 cgaatcttcc agatgtgtat gcagtaggag atgctacact tgcttattct atcccagcac
    12421 gcaaaaaaat gccgatcgct ttagcgactg ttgcacgtag agaagcacgt tatgtagtaa
    12481 aacatctttt tgaagaagta ccaaacaaac cgttcggcgg agtcgtaggt tcctctgcat
    12541 taagtgtctt tgactatcat tttgcagcaa gcggattgaa tagctttaca gcagcaaaag
    12601 caggagtaga tgtacgagtg tcttattatg aagatacgtt acgaccaaaa tatgtaccag
    12661 aagaacaagg aaatccaaaa gtgtccatcc aattattctc ggatccaatg acccatcaat
    12721 tgctaggagg agcagtatta tctacctatg atgttacagg tcaaggaaat gtattggcac
    12781 tagcgatcca aaacaaactg actttagaag aactagcaga agctgacttc ttcttccagc
    12841 caggctttga ccgacaatgg agcatgttga atctagccgc tcaacaagcg ttaggagaag
    12901 aaccatttgt agaataagaa aacgaaatat ttgaaaagag gttgtgaaaa agctgccttg
    12961 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggctggac
    13021 ttattaccgc agatgcaagc ttttgaacac cgtttatttt caaaaaaggg actgtgacac
    13081 gatttgtgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
    13141 ttttatggtt aaatatactt ttgaacttaa attaaagatc gttcatgatt atttggatgg
    13201 caaaggcgat tggaccgtaa aaatccagat caagagattg aagcggaaat gcttaaaatt
    13261 cgggagaagc acaaggatta tggctgttta cggatgacca atgaactaag aaaccgcggt
    13321 ttctctatca ataaaaagaa agttcagcgc ttgatcaaga agctaggaat aaaagtaacg
    13381 gcatacacgc gtaaatcacg tcgctacaat tcttatcgag gaaaagt
//
LOCUS       DAFFFQ010000080        13395 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000080
VERSION     DAFFFQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.080"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(14..706)
                     /locus_tag="LQB69_002355"
     CDS             complement(14..706)
                     /locus_tag="LQB69_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3526493.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="LQB69_002356"
     CDS             complement(1143..1712)
                     /locus_tag="LQB69_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3526494.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="LQB69_002357"
     CDS             complement(1786..2109)
                     /locus_tag="LQB69_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526495.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="LQB69_002358"
     CDS             complement(2263..3075)
                     /locus_tag="LQB69_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526496.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="LQB69_002359"
     CDS             3429..4277
                     /locus_tag="LQB69_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL3526497.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="LQB69_002360"
     CDS             4422..5363
                     /locus_tag="LQB69_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL3526498.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREIPFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="LQB69_002361"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="LQB69_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LQB69_002362"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="LQB69_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL3526499.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="LQB69_002363"
     CDS             complement(9827..12013)
                     /locus_tag="LQB69_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL3526500.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LQB69_002364"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="LQB69_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL3526501.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="LQB69_002365"
     CDS             complement(12235..12675)
                     /locus_tag="LQB69_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL3526502.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="LQB69_002366"
     CDS             complement(12749..13288)
                     /locus_tag="LQB69_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3526503.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3761 a         2810 c         2382 g         4442 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatccc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaa
//
LOCUS       DAFFFQ010000081        13346 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000081
VERSION     DAFFFQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.081"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(45..1364)
                     /locus_tag="LQB69_002367"
     CDS             complement(45..1364)
                     /locus_tag="LQB69_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3526504.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="LQB69_002368"
     CDS             complement(1361..2014)
                     /locus_tag="LQB69_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526505.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="LQB69_002369"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="LQB69_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="LQB69_002370"
     CDS             complement(2724..3440)
                     /locus_tag="LQB69_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL3526506.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="LQB69_002371"
     CDS             complement(3490..3699)
                     /locus_tag="LQB69_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526507.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="LQB69_002372"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="LQB69_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="LQB69_002373"
     CDS             complement(5356..6387)
                     /locus_tag="LQB69_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526508.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="LQB69_002374"
     CDS             complement(6394..7224)
                     /locus_tag="LQB69_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526509.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="LQB69_002375"
     CDS             complement(7221..8036)
                     /locus_tag="LQB69_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3526510.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="LQB69_002376"
     CDS             complement(8033..8857)
                     /locus_tag="LQB69_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL3526511.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="LQB69_002377"
     CDS             complement(8847..9947)
                     /locus_tag="LQB69_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL3526512.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="LQB69_002378"
     CDS             10125..10910
                     /locus_tag="LQB69_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL3526513.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="LQB69_002379"
     CDS             10943..11326
                     /locus_tag="LQB69_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL3526514.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="LQB69_002380"
     CDS             11402..11533
                     /locus_tag="LQB69_002380"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3526515.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="LQB69_002381"
     CDS             11502..11738
                     /locus_tag="LQB69_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3526516.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="LQB69_002382"
     CDS             complement(11816..12787)
                     /locus_tag="LQB69_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL3526517.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2911 c         2306 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DAFFFQ010000082        13333 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000082
VERSION     DAFFFQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.082"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="LQB69_002383"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="LQB69_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL3526518.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="LQB69_002384"
     CDS             complement(738..1460)
                     /locus_tag="LQB69_002384"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL3526519.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="LQB69_002385"
     CDS             complement(1596..2477)
                     /locus_tag="LQB69_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL3526520.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LQB69_002386"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="LQB69_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL3526521.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVVLFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="LQB69_002387"
     CDS             3717..4196
                     /locus_tag="LQB69_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL3526522.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="LQB69_002388"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="LQB69_002388"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL3526523.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="LQB69_002389"
     CDS             5497..5625
                     /locus_tag="LQB69_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL3526524.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="LQB69_002390"
     CDS             5629..6174
                     /locus_tag="LQB69_002390"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL3526525.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="LQB69_002391"
     CDS             complement(6628..8319)
                     /locus_tag="LQB69_002391"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL3526526.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LQB69_002392"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="LQB69_002392"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL3526527.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LQB69_002393"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="LQB69_002393"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL3526528.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LQB69_002394"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="LQB69_002394"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL3526529.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="LQB69_002395"
     CDS             12601..13302
                     /locus_tag="LQB69_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3526530.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3753 a         2710 c         2367 g         4503 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctaagaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatac tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taa
//
LOCUS       DAFFFQ010000083        13043 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000083
VERSION     DAFFFQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.083"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(77..149)
                     /locus_tag="LQB69_002396"
     tRNA            complement(77..149)
                     /locus_tag="LQB69_002396"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="LQB69_002397"
     CDS             530..1099
                     /locus_tag="LQB69_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL3526531.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="LQB69_002398"
     CDS             1086..1946
                     /locus_tag="LQB69_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL3526532.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="LQB69_002399"
     CDS             2136..2840
                     /locus_tag="LQB69_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526533.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="LQB69_002400"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="LQB69_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL3526534.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="LQB69_002401"
     CDS             4677..5993
                     /locus_tag="LQB69_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526535.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="LQB69_002402"
     CDS             5994..6863
                     /locus_tag="LQB69_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL3526536.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="LQB69_002403"
     CDS             6918..7727
                     /locus_tag="LQB69_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL3526537.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="LQB69_002404"
     CDS             complement(7830..8459)
                     /locus_tag="LQB69_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL3526538.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="LQB69_002405"
     CDS             8629..9921
                     /locus_tag="LQB69_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526539.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="LQB69_002406"
     CDS             complement(9997..10659)
                     /locus_tag="LQB69_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL3526540.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="LQB69_002407"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="LQB69_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL3526541.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12835
                     /gene="groL"
                     /locus_tag="LQB69_002408"
     CDS             11219..12835
                     /gene="groL"
                     /locus_tag="LQB69_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL3526542.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAMDPSMMGGMM"
BASE COUNT         4337 a         2251 c         2822 g         3633 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgcctg caatggatcc atcaatgatg ggcggtatga tgtaagaaaa
    12841 agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga ggctggaaca
    12901 gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc aaatttttgt
    12961 gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac gtgtttagag
    13021 cgtgagacaa aagtgttctt tac
//
LOCUS       DAFFFQ010000084        13025 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000084
VERSION     DAFFFQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.084"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            7..1032
                     /locus_tag="LQB69_002409"
     CDS             7..1032
                     /locus_tag="LQB69_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL3526543.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            1029..3212
                     /locus_tag="LQB69_002410"
     CDS             1029..3212
                     /locus_tag="LQB69_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL3526544.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            3212..4342
                     /locus_tag="LQB69_002411"
     CDS             3212..4342
                     /locus_tag="LQB69_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL3526545.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            4339..5016
                     /gene="pgmB"
                     /locus_tag="LQB69_002412"
     CDS             4339..5016
                     /gene="pgmB"
                     /locus_tag="LQB69_002412"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL3526546.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            5100..5627
                     /gene="msrA"
                     /locus_tag="LQB69_002413"
     CDS             5100..5627
                     /gene="msrA"
                     /locus_tag="LQB69_002413"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL3526547.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5828..6514)
                     /locus_tag="LQB69_002414"
     CDS             complement(5828..6514)
                     /locus_tag="LQB69_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL3526548.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            6594..8132
                     /locus_tag="LQB69_002415"
     CDS             6594..8132
                     /locus_tag="LQB69_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL3526549.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8252..8719
                     /locus_tag="LQB69_002416"
     CDS             8252..8719
                     /locus_tag="LQB69_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL3526550.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            9238..9681
                     /gene="rplM"
                     /locus_tag="LQB69_002417"
     CDS             9238..9681
                     /gene="rplM"
                     /locus_tag="LQB69_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL3526551.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            9697..10089
                     /gene="rpsI"
                     /locus_tag="LQB69_002418"
     CDS             9697..10089
                     /gene="rpsI"
                     /locus_tag="LQB69_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL3526552.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(10178..11404)
                     /locus_tag="LQB69_002419"
     CDS             complement(10178..11404)
                     /locus_tag="LQB69_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3526553.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(11476..11634)
                     /locus_tag="LQB69_002420"
     CDS             complement(11476..11634)
                     /locus_tag="LQB69_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526554.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            12322..>13025
                     /locus_tag="LQB69_002421"
     CDS             12322..>13025
                     /locus_tag="LQB69_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL3526555.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIF"
BASE COUNT         4250 a         2371 c         2693 g         3711 t
ORIGIN      
        1 aaataaatga agaaagtagc aatcataggc gctggacaag tagcagaaaa agtacatgct
       61 gcttattatc aaaaaagatc agatgtctta gaattggccg ctgtggttga tccagtcagc
      121 cagcgagcag aagacttttg ccgacgtaca ggattttctc atgcgtatac atcagttaca
      181 caaatgctga atgagatttc tcccgatatc gtgagtgtct gtacacccaa tcgctttcac
      241 ttcgaacatg tattggctgc cctcgaagct aatgcttcag tgatgtgcga gaaaccacca
      301 gctatgacag ctgatcaagc aagaaagatg gcagagacag ccgataaaaa cgggcagatt
      361 ttggcttatg gattccagca tcgtttttct gacgaagcaa caatgatcaa gaatcatctt
      421 gatgttctcg gggatattta ttacgtagaa gcgaatgcgc ttcgtcggtc tggtgttcca
      481 ggctggggaa cgtttatcga caaagaactt caaggaggcg ggcctttgat cgattatggc
      541 attcacatgc ttgatacagc gatgtatatt actggatttc ctaaagtaaa aagcgttcgg
      601 gcgtacagct tccaaaaaat tggtacgaaa aaaaattcag ggaaattcgg gaaatgggat
      661 ccaagcaaat tcacagtaga agatgcaatt tttggcacac tggaattaga aaacggcaag
      721 atcatccaac tgaatacttc gtttgctttg aatatcagag aagacaaact gaatgtggct
      781 ttttgtggcg atcaggcagg tgctaatctg tatccaggcg aactattcga agatgtagat
      841 ggaaagctgg tttccttaca aaagaccacg ccagttaatg acccacatga actaagtatg
      901 gctcattttg tgaatcgggc gctcggtgac cagactcaga tgattgcaga tggctggcaa
      961 ggatatgtca tccagaatct ggtggaaaat ttatatcgtt cggcagaaac aggagaagtt
     1021 attaccttat gaaagtagaa gaaaaacagt ttgatttaga gtatatcaat aagtatgcga
     1081 gtcagatgac cattggaaat ggattttttg gtatcagagg aagccaggag gaagactatc
     1141 ggaatcaagt aaggggaatg tttgcagctg gtgtatataa ccgaccttta ggagcagaat
     1201 cagccgagtt agtgaatctc ccagatgtca tcagatggga aataaaaatc gacggtgaac
     1261 atttttctct tgagacgatg aaagttcttg catacgagcg ggcactagat ttagaaatgg
     1321 gagagcttaa acgctatgcg ctcattgaaa ctgctcaagg aaaaaaagtg gaagtcatta
     1381 cttgtcgtgt tgctgatcaa aataatctcc atcaggcagc cctgaaagtg gtcatcaagc
     1441 cgttgaccga agactgccaa gtagatgtga aaacgggtat cgatggacag caaaccaatt
     1501 ttggtacaca gcagttggtt gaaaaagaac tgcgagtatt tggtgacgat ttgatcagtg
     1561 catcctacca gacaacagaa agtaaaattg atattgggat tgcagctaaa ttcaataaag
     1621 caggtgtatt ccaagcaaaa aatcgccgga tcaccgcttc cttttcagaa aagatcagaa
     1681 aaaatacgtc ctttacgctg gagaagatca tcgtgctctc tacctctctt cagcaaacag
     1741 acccgtttaa gaaaagtatt gcccaagccc agaaaagcgt tagttataca gaaattttag
     1801 agcagtcgat gtcttattgg acagattttt ggcatcagca tcggataacg atccaaggcg
     1861 attcttcttt cgatcagtta gcgatggact tcgctgctta ccatttacag attatgactc
     1921 cccgcaatga tccaagtgtt agtgtagggg cgaagggact aactggcgaa ggttataaag
     1981 ggcatgtttt ctgggatacg gaaattttta tcctgccttt ctttttatat cagtttcctg
     2041 aagtagcaaa gaatttgctg ctgtaccgtt atgatcgatt agaaggcgca cggcaaaaag
     2101 caagagatta tcactatcaa ggtgcacttt tcccatggga aagcgcacac actggtcagg
     2161 aagagacacc caaatttgct gcattgaata tcaaaaccgg aacaagacaa gaagttgctt
     2221 cagctgccgc agaacatcat atcagtgcag accttgctta tgccgttcaa gagtatgtcc
     2281 aagcaacaga ggatcaatcg tttttagaaa caaagggaaa aacgctcatt catgaaacag
     2341 cagctttttg gctgagcaga gctgtaaaag tcgatgatca tttagagata cacagagtca
     2401 ttggtccaga tgaatacaca gaatatatcg acaataatgc atatacgaac tatctggcgc
     2461 attataatgt ggccttagct gttaaatatc aagctggcga tccgcttttt ttgaaagaat
     2521 gtcaaaattt tttagataag ttatatttgc cagtgcctaa caaagaacag ctgattccgc
     2581 aagatgacac gtatctcagt aagccagtta ttgatttgac cacttacaaa gaacgtcctg
     2641 gcagccagtc tattttactt gattatgccc gtagcgaagt aaatgaactc caagtcagca
     2701 agcaagcaga tcttgtcatg ctgttatact tattacctga gtatttttct caaccaatcg
     2761 tttctaagaa tttgcattac tatgaaaaca aaacaatcca cgactcatcg ttgagtaaag
     2821 cgattcacgc catagaggct tgccgtatcc aagaaatgga atgggcgtat catttattcc
     2881 aagaagcgtg cctgattgat ttaggaccag atccgcattc atcagatgac ggaattcatg
     2941 ctgcttcatt aggagcacta tggctagcag ttatatttgg tttttcaggt attacagtaa
     3001 cgaatggaac tctttctatt tctccaagac ttccttcagc atgggaaaaa atttcttttc
     3061 cttttatttg gaaaaatcac aggttgctat ttgttttgac aaaaaaacaa atagtaataa
     3121 aaaaagagac gaaagaaaca ttacctattt ttgtcaatgg agaccaatac cagctggaaa
     3181 aagaactgat cttggaacgg aggggaaaat aatgcgtatt ctgattgcgc cagattcttt
     3241 caaagaaagt ctgacagcct tagaggcagc acaagccatt caatcaggtt ttcaagaagt
     3301 ttttgttcag gctgaatatg atctgctacc tataggcgat ggaggagaag gaacggtttc
     3361 atcattagca gacagtttaa ggttgaagcg gcaaacagca gtcgttacta atgcatttgg
     3421 caaaaacaaa gaaatccagt acgcttcaga cggtcaaact gcgatttttg aaatggcaga
     3481 aatcgtcgga ttgaaagatg tcccactaaa ccaacaacag ccacttaggt tatccacgcg
     3541 aggagtagga gaactgatac gacaattgtt gcaacaaggt gttcgacaca tcatcatcgg
     3601 cgttggcgga agtagtacca acgatggcgg catcggcatg gcagaaggtc taggttatgc
     3661 tttttacgat caagataatc aaaaagtaga agcactggga gaaaatctag gtaaaattgc
     3721 tacagttgat aaaacgaacg tgcctattga gctgattaaa gccaaaatca ctgtcattac
     3781 tgatgttact aatcttttat gcggaaaaga aggcgcttct tttgttttcg gaccacaaaa
     3841 aggcttgaaa gaaaatgaat tagcagtggc tgaccaagcc atgcatgatt tttatatgag
     3901 atttgcccct caagtaatcg aggcatcggg cagtagtgct ggtggtggaa tgggtgcagg
     3961 actgttagca tttgcttcag ggaaaatagt ctctggcatc gactacgtat tggatttact
     4021 ggagttcgat aaacgggtac agcaagcaga cctcgtaatc gtaggcgaag gacgtctaga
     4081 tggacaaagt ctaaaaggaa aagcgccagt tgggattgct agaagagtac cagagggcgt
     4141 tcctgtgata gctatttgcg gaagtgttgg taaaggaagc gaaaaagcag cagatgttgg
     4201 catttcggcc gtttttccga tccttcctgc agctgtgcct ttacaagaag ctttacagca
     4261 agccactgat aatcttcatc gcacagctaa aaacatcgca tctttacttc aagtcgttca
     4321 ttttcaagga ggtaatcgat gaaaatcaaa gcagcactat tcgatttaga tggcgttctt
     4381 gtagacacag ctcgttatca ttacgaagcg tggctggttt tagctaacca gctgtccatt
     4441 ccattcactg agaaagaaaa cgagcaactg aaaggaatat ccagaaccga gtcactagag
     4501 cgattgctgt catttggaaa aatggaacag aaattctcag aaaaagagaa atcagccttt
     4561 gctgagcaaa aaaacaatct atatcttcaa gcgattcaaa aaatggatga aacatccgtt
     4621 ttgcctggtg caatagctgt tcttgagtac ttgaaaaaaa caaacatcaa gatcggattg
     4681 ggatcagcaa gcaaaaatgc acgcctcatt ttagaaaaaa cgaatttgac aagttatttt
     4741 gatgtcctta ttgacggcac gcaagtatca aaagcaaaac ctgatccaga agtttttctc
     4801 aaaggagcac agcaattgaa tgtaccgcca aatgcatgct tggtcattga agattcagaa
     4861 gctggttgtc aagcagcttt ggctggtaac atgcatgtat tgggaatagg tgaaaacata
     4921 aatcttcctt ctgctgaata tgtgattcct gatttaaccg tttttgatca agtacgttcg
     4981 ttttggcatt tatcagaagc cgtatcacgt atataatagt ttccgttttt tctgtcacaa
     5041 gcaaaacgtt atcctccttt tggaaatcaa ggtatgatga gaaaaaaggg ggattttata
     5101 tgacaaaaaa agcagttttc gcaggcggat gcttttggtg tatggtcaaa ccatttgaag
     5161 agcagccagg gatcatttct gtgacatccg gttatactgg cgggcatgta ccaaatccaa
     5221 cttatgaaca agtaacaaca ggcacaacag ggcatacaga agcagtagag atcgaatatg
     5281 atccagaagt tatttcttat gatcagttgg tggaaatcta ttggcagcaa accgatccga
     5341 ctgacgcatt aggacagttc gctgatcgcg gtgattctta tcgtccagtg atctactata
     5401 atgatgaaga agaacgaaat attgcagagc gttcaaaaca agcattgcaa gacagcgggc
     5461 gattcgataa gccaatcgtg acaaaaatcg aaccaagaag cgaattttat cctgcagaag
     5521 attaccatca ggattattac aagaaaaaca agctccatta caatctttat cgagaaggtt
     5581 ctggacgtgc aggtttcctt cgtcgacatt ggggcgtcaa gaaataagaa gccgaaaaag
     5641 ctacttatgg aacaccgtat atcagaggtt gtaaaaccag cattttgagc taatactgag
     5701 ggtcagctga ttgaacacca tttagtggag aggttgtgaa cctgtcgcct agctccaagt
     5761 cacaaggatt tggtaagaga acactatata tcagaggttg tgacaagtcg tttgtcacaa
     5821 ccttttttca atacctaaga tgaaggacaa tcagcaacgc actgctcaaa aaaggaatga
     5881 aaggcagctt ttccaacttt tttttatgac agataaagta aacagaaaaa acggatattc
     5941 caaacagact agcaagaaac aagatctgaa gcaactcttc tagagacaag ccaccactcc
     6001 aagaaagcat caacagagta tcgccagctc cgagatagga ttgaccatag cgaaggcaca
     6061 caccgcctac gataaacgtt aacagcacac tttgccattg gaagacgccg gcctgcaacc
     6121 aaaaaatcca taaaattgcc caagaacaga atagcgtgtg agaatggacc atgaagtaaa
     6181 ataaatccat caacgaaaat agcaaagccg aaagcatcca tatgaacgtc gaaaaatttc
     6241 ttgcggaaga aaaagtaaag aaccaggcaa aaagaccacc acataaggct tctgccaaca
     6301 aatagtagaa gggcaaccga cacctgcaat agcggcagcg aaatcgttgg acgatgatcg
     6361 agagaatcgg aatcaattca taccaagaaa gcgaacgaca gcatttgaca caatgagatc
     6421 taggataaat aatagagtga cctaacggaa tccgctgggc ggttaaacat aaaaatgatc
     6481 cgatacagca accaatgata aaataaagta acacgccatc tccccctttt caaaaccaag
     6541 atacgcaagc ttaaactaaa tttttgtaca tacataaaat ttgaggtgaa aaaatgaacg
     6601 tttcaatcgg attagatatc ggaaccacac aaaccaaagc agttgctttc acagatgaag
     6661 cccaagaagc agcatccgct tattttcgct accctttgat ccaagaaaca caagggatgg
     6721 cagaacaaga tcaggaattg atctttcaag gtgtctgcac cgttataaaa gaagtaatta
     6781 aacagttgcc ttcagacaca atcattcaat ttgtctcctt ctcctctgcg atgcatggcc
     6841 tgatattgtt ggatgaagag aaaacaccgt tatctcgaat gatcacttgg gcagataacc
     6901 gagcagcgga cgaagcagaa aaactgaaat ataccgtgct tggcaaagaa ttttatcggc
     6961 agactgggac gccgattcat ccaatgacac cattgaacaa aatcaagtgg ctgcaaaaca
     7021 gccaacctga attattcaaa aaaactgctt attttgtcgg tatcaaagac tatatctttt
     7081 atagattgtt tggcgaactg atcagcgact acggaacagc ttcaggaact ggatattttg
     7141 atatccatga attccaatgg gcagaagaag tgcttgacta cttgaacatt cgaatagagc
     7201 aattgccgca agtctttcct tccacctatc aagtaacagg tctaccatca aaaacagcag
     7261 cagaactagg tttgccaaat gatatatcat ttgttttggg aggggcagac gggccgcttt
     7321 ctaatctagg gttaggtgct tttggtgata cgatcgccgc tttgaccgta ggaacgagcg
     7381 gagcacttcg ttttattact actcagcctc agttccatcc agaaatggag acgttttgtt
     7441 atgttctgga tcagaaacat tgggtagtgg gaggagcgac tagcaatggt gctggcatct
     7501 ttgattgggc agggcataca tttttacagg aagtcgttca atctgctaaa gaaaaaggag
     7561 aaaatccata tgatgcactg ctttcggcta tcgcttttgt gccagcgggt gccaatggtc
     7621 tgatttttca tccctattta cttggtgagc gagcaccgct ttgggacgca gatgcgtttg
     7681 gcagttttat cggtttacag cggcaacata cagaaaaaga aatgatgaag gcgattctag
     7741 aagggatctg tctcaacctt tatcggattt taaaaggagt ttgccaggaa aatcagctca
     7801 cagagatccg tgctacaggc gggtttgctc aatctccgat ttttcgtcag atgatggctg
     7861 atgtactggg ttacccgctt gttttcccta ctgtcacaga agcatccgca ttaggagctg
     7921 tgattttagg ctggagaagc atcggaaaga tccaaagtat cagtgaagca caggaattga
     7981 tagaattgaa agagcaagtt ggagtacaag aaactgatca taaaaaatat cagaaaatct
     8041 atccattatt tgtttcgaca caaaagcatt tagcacagac ttatcagcaa tttgctcaac
     8101 tgcgagaaga attagctgat tctgaaaaat gattggcttt acggataaaa aagcgaactg
     8161 aaaatcgttc tcaggtaaat gattttgttg tacttttcat tgaaagtcta ctattatttt
     8221 aagtgtaaga aaatgttagg aggcaagaaa gatgaaattc caacaaacca aagaagtatt
     8281 aaatcaatta gttgcagatc taagccaaat gtctgttgtt gttcaccaaa cacattggta
     8341 catgagagga ccaggtttcc ttacattgca cccaatgatg gatgagttca tggatgattt
     8401 gaacgctcag ttagatgaag tatccgaacg cttgatcaca ttagacggtt caccttattc
     8461 tacattgcgt gaatttgcag ataacacaaa aatccctgat gaaattggcc gctgggatcg
     8521 tactattcca gaacgtttgg aaacacttgt tgcaggctat cgttatctag cagatcttta
     8581 ccaaaaagga atcgaagttt ctggagaaga aggcgacgat ccaacacaag atatctttat
     8641 cgaattcaaa acagccacag aaaaacgtat ctggatgatc caagctcatt tgggcaaagc
     8701 gccagagatc gacgcgtaaa aactgttgtt gaacttgacc gaaaaagagg ttgtgaaatc
     8761 agcgttttga cctgagtatt agagaaacaa acgtcaaaat agttcctcat attttgccgt
     8821 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttaccaagag gttgtaagcc
     8881 tgccgttcag ctccgagtca caagaaacaa ttttaaaaaa cagcttctta tgttttatgg
     8941 atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcaa aataaggggt
     9001 tgtgacaaga cttttgtcac agcctctttt tgtttttcat tttgacagga aacgaccctt
     9061 ttatataaaa gaaagaaaag aagtaaagtt tcatataaat ttttcaatca agcagaaaaa
     9121 cgttgacatt gactagatta gcgggtaata tgttaaatgg tattgtttgc cccacaaaga
     9181 gccccggaaa ctcgaagtgt atgtcaaaca tcagaactta aattggagga aaataacgtg
     9241 cgtacaacat atatggccaa accaggcgaa gtagaacgta aatggtatgt agtagacgca
     9301 actgatgttc cattgggacg tctttcaaca gttgttgcat ctgtactacg tggtaaaaat
     9361 aaaccaactt tcacacctca tttggatact ggtgatttcg tcatcgtcat caatgcagac
     9421 aaagtgaaat taacaggtaa aaaagcgact gacaaaattt attaccgtca cagcaactac
     9481 ccaggaggat tgaaatcaat cactgctggc gaattgcgtg ctaaaaattc tcgtcgtttg
     9541 atcgaaactt ctgtaaaagg tatgcttcct aaaaacactt taggccgcaa acaatttaca
     9601 aaattgaacg tatacggtgg agcagaacat ccacatgcag cacaacaacc agaagtttta
     9661 gacatcacaa acttaatcta ataggaggga atttcattgg cacaagttca atatatcggc
     9721 acaggccgtc gtaaaaacgc agttgctcgc gtacgtctag taccaggaac tggcaaaatt
     9781 actgtaaata aaaaagatgt tgaagaatac attccacatg ctgacttgcg tgaagtaatc
     9841 aatcaacctt tcgctgttac tgaaacaaaa ggcgcatatg acgtatttgt taacgtaaac
     9901 ggcggaggat acgctggaca atcaggagct atccgtcatg gtatcgctcg tgcattgcta
     9961 gaagttgatc ctgacttccg tttagctctt aaacgtgctg gcttacttac tcgtgacgca
    10021 cgtatggtcg aacgtaaaaa accaggtctc aaaaaggctc gtaaagcatc tcaattctct
    10081 aaacgttaat attatttata tatcaatgtt tcaaggcact tttcaattgt ttgaaaagtg
    10141 tctttttctt gtttaagaac acatttaaga acacatacta atcacattta ttcaatcctc
    10201 tctgaaaggc ttcaaaaaca gttttatcta ctacatctgg tgtatttacg tatattctcg
    10261 ttgtagaaat atctgagtgt gttaaagcac tagatatttg attcatattt gctccgcctt
    10321 gtctagctat agttgcatag gtatgacgaa gcttatgtgg agataaatga gccaattcag
    10381 gatgtcgtct tttaattgaa ttgattcgat agtttaggta atcagcgtga acagggcaat
    10441 ttacttctcc ggatggttta gtataggtaa agagatactg atctggtgtt tgattaattt
    10501 ttaatctcaa caattgctca gcttgcactg atttccattt gcttagtaat gccattacaa
    10561 tttcgggtac ttgaattacg gtatcctttc gaccttttgt aaatttcttt ttttgatatt
    10621 tatccaaagc atgtttaagg cgaaccgttt gtttctcaaa gtcgatatat ttccattgca
    10681 aagcatacgt ctcacttttt ctatcaccaa gatatagggt aagcatgaaa agagtatagt
    10741 catgaagagt aagcaagtga ttattcaagt cagtgtttac tgcttctatc caagcaataa
    10801 gctcttctga cgataatgct tgattacctt cccgtatcct tctttcttct aaagcaattt
    10861 ttttaggtgc agtgatacat tgaataattt tggtagttcg gttaaattca atataattta
    10921 gtatttctgc aatatcaaac atagaacgga catatccttt gataattttt atatttgcgt
    10981 attctttcga tttttttgtt aattcatctg atattatctc agtatttgaa tttagatagt
    11041 tcattgcata ctcaccaaac attggaagga tgtgcaatcg gaatatatct ttagtattac
    11101 taattgttac gtaacttgga gctctttttg atctagtagt ctgacctagt tcatatctag
    11161 gaagccattt tgattcatag aattccttaa attttatttt tccttttaat tctagtgaat
    11221 ttgcattctc ctctcttaaa actgtttcaa ttttcctttt cattgcattt tcggctttaa
    11281 tagccaattg cctagttgga aatacttttt gatacttttc tgatgtaatt ccagtgattt
    11341 ctctgaattt ttttggatac ctcagattag ttttatattt cccatttgaa agttttgtag
    11401 gcaaaattcc tcactccctt aaaaagtaag gcagtatatt tcgctattct tagtatatgc
    11461 ggcgagaaga aattttcaaa ttgttttaaa tgtattcata aaatctttga tatcctctat
    11521 gtcaaagcga actataccgt caattctgat tactggaaaa ttatatatac gaatccactt
    11581 atctaaagta ttattatcaa ttcctagaaa ctcacaggtt tgttttttat tcaagtagac
    11641 tctcaatttt ataaaatcat ctcctaaaaa agattagaat ttttgaattg ctttgggtaa
    11701 gcacatagga atttcacctc tccccagttt acggcgagcc ttacggaagt atcattattt
    11761 gctgctattg tcatggcatt ttgttttccc agaacaaaaa gaaatctcta agtatttgtc
    11821 gctaatcagg tcatggcgta cttgagtttc tagctcgaat agcagggatc gttattcaaa
    11881 aattgtattt aattgtcaat gatcaaaaat aggttttttc ctattcacta ttaataacga
    11941 taaacatgta taaaaagaca cactacgaaa aaagttaaat ggagtttact gattttcata
    12001 tttgtatatg gaacagacat agtcttgaat aatttttata aatgttttga tgcttatcaa
    12061 cttattcaac ccgctatcca ctttaagtat aatttttcca tacgccaata aagtagtttt
    12121 aggagcatgt tctcatttga gaacgctcct tttttatata ttttgaaagc tacttactat
    12181 acgcgtagtt tcaaatagtt ttgtcattct aatcttcagc aacagaaaaa aatctaaatt
    12241 ataaaattta gggttgacta aaatataatt tttgtatacc atatgatcgt gataatcatt
    12301 atcatttaga aggagaaagt tatgttagaa gtgaaaaagc ttaatgtttg ttataacgat
    12361 tttttagcat tgaacgagat ttctttaaaa atacaagaag gtgctttaac aggtattatt
    12421 ggtccaaatg gtgctggaaa atctacattg cttaaagcaa tgttaaatat tatccctcat
    12481 cagggagagg tctccattaa taatgaagat atcaataaaa aactggtgaa gattgcatat
    12541 gtagagcaaa aagctgatat tgattttact tttccaataa aagtaaaaga atgtgtatct
    12601 atgggaacat atgccggaat gaaagttttt caaagaatta aaaatgcaga atggcaaaga
    12661 gtttcaaagg ctttggaaaa ggtcgatatg ggaaaatatt ctaatcatca aataggagaa
    12721 ttatctggag gacaatttca acgtgtactt ttagcaagat gtcttgctca aaatgcggat
    12781 ttcatatttt tagatgaacc atttgtaggg atagacttag ttagtgaacg aataattatg
    12841 gatacgttaa aagaattgaa acatcaggga aaaacagtgc taattgtgca tcatgattta
    12901 agcaaggtaa agaagtattt tgataatatt attcttttga atcgaaatct aattgctcac
    12961 ggaagtgtag aatctgtatt taatgaagag aatttaaaaa aagcctatgg agatactata
    13021 tttat
//
LOCUS       DAFFFQ010000085        12597 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000085
VERSION     DAFFFQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.085"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(10..159)
                     /locus_tag="LQB69_002422"
     CDS             complement(10..159)
                     /locus_tag="LQB69_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526556.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            555..755
                     /locus_tag="LQB69_002423"
     CDS             555..755
                     /locus_tag="LQB69_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3526557.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1323..1886
                     /locus_tag="LQB69_002424"
     CDS             1323..1886
                     /locus_tag="LQB69_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526558.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1947..2291)
                     /locus_tag="LQB69_002425"
     CDS             complement(1947..2291)
                     /locus_tag="LQB69_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526559.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(2388..2633)
                     /locus_tag="LQB69_002426"
     CDS             complement(2388..2633)
                     /locus_tag="LQB69_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526560.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2703..2957)
                     /locus_tag="LQB69_002427"
     CDS             complement(2703..2957)
                     /locus_tag="LQB69_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526561.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3121..3444)
                     /locus_tag="LQB69_002428"
     CDS             complement(3121..3444)
                     /locus_tag="LQB69_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526562.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3631..3704
                     /locus_tag="LQB69_002429"
     tRNA            3631..3704
                     /locus_tag="LQB69_002429"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3665..3667,aa:Ile,seq:cat)
     gene            complement(4089..4586)
                     /locus_tag="LQB69_002430"
     CDS             complement(4089..4586)
                     /locus_tag="LQB69_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL3526563.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4626..5435)
                     /locus_tag="LQB69_002431"
     CDS             complement(4626..5435)
                     /locus_tag="LQB69_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3526564.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5769..6626
                     /locus_tag="LQB69_002432"
     CDS             5769..6626
                     /locus_tag="LQB69_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL3526565.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6619..7521
                     /locus_tag="LQB69_002433"
     CDS             6619..7521
                     /locus_tag="LQB69_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL3526566.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7554..7904
                     /locus_tag="LQB69_002434"
     CDS             7554..7904
                     /locus_tag="LQB69_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL3526567.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7953..8876
                     /locus_tag="LQB69_002435"
     CDS             7953..8876
                     /locus_tag="LQB69_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL3526568.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8954..9847
                     /locus_tag="LQB69_002436"
     CDS             8954..9847
                     /locus_tag="LQB69_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526569.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9885..10448
                     /gene="amaP"
                     /locus_tag="LQB69_002437"
     CDS             9885..10448
                     /gene="amaP"
                     /locus_tag="LQB69_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL3526570.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10462..10650
                     /locus_tag="LQB69_002438"
     CDS             10462..10650
                     /locus_tag="LQB69_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL3526571.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10663..11565
                     /locus_tag="LQB69_002439"
     CDS             10663..11565
                     /locus_tag="LQB69_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3526572.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11597..11839
                     /locus_tag="LQB69_002440"
     CDS             11597..11839
                     /locus_tag="LQB69_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3526573.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12095..12466)
                     /locus_tag="LQB69_002441"
     CDS             complement(12095..12466)
                     /locus_tag="LQB69_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526574.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4462 a         2113 c         2365 g         3657 t
ORIGIN      
        1 ttccactcct tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt
       61 cgctacatga ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa
      121 ggttactcga tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa
      181 tacttgctga aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca
      241 attgaaaggt tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata
      301 aacaagaaaa caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac
      361 aatagaagac aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat
      421 gatatattaa atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata
      481 aaactccttt tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca
      541 ggaggaattt atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg
      601 gctttatcac tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg
      661 acggcttcaa aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac
      721 gtggccctca agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt
      781 tcgaaagaaa agacggtttt tttgtacgaa aaaattcatt ggtcgttgaa tgggctgaat
      841 agtagtgtat aaaacaattt tgtggagcgt gaagctttta tggtagagga aaatacgaat
      901 aaagtgatgt tgataaaaag attcctatct tgaatatgaa tcgagatgtc ttgaaaatga
      961 atgagtttga ccaatcatta tcctatcgaa atttacagtt tcgttcgatt ggtctgtcaa
     1021 ttaatgaaaa agtaaaaaag aatatattct aggaagaggg cggcacatct aaatttttaa
     1081 gtaacggaca gttcaaatat caagcattga aagaggctat cctactgttt gaaggaatac
     1141 gaatatataa aaacgacaaa gaggaaaaag tagttcatta aatcccttca agtgatgaaa
     1201 gaatgagatc taataaaacc tgatctttca tctgtttttt taataacgta ataatttttt
     1261 tattttttgt gctttcgccg ttattgtaga gtgatttagc attcttattt ttaaaaataa
     1321 ctgtgttata tattaaaaaa ggcataatag ttataagctt ttttaaagaa tatttagaaa
     1381 ttattaattt tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg
     1441 gggggaacct tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag
     1501 gttgttctga ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag
     1561 aggagttaaa atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg
     1621 gaatattagg cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa
     1681 gcaacagaaa aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag
     1741 acgtcgaaga tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag
     1801 ttggcgatga accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc
     1861 ctaaaactta cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga
     1921 ggagatagaa ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt
     1981 gctaaacatt ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct
     2041 acagtaggta aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac
     2101 tctggtgctg ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg
     2161 aataatctat aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag
     2221 ccatttcctc ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc
     2281 tttgaaatca atttttcatg ccataatcac atagaaatgt ctcctttata tatagattgt
     2341 taaccttaga cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg
     2401 cttgctgttt ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata
     2461 tagattaatt caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat
     2521 aaaggaatac ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag
     2581 aaattcagac gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca
     2641 cctttcatta tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt
     2701 actcaatgtt ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc
     2761 agtcctgata attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact
     2821 ttccacataa agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc
     2881 caattcataa gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact
     2941 ctcaccaacg taatcatgta acggactcct caacgattca tataataaaa gataacatgt
     3001 tattgcatac atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa
     3061 tgaaaaagca gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat
     3121 ctatcgagaa aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac
     3181 gatcgcattc actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac
     3241 aaatactttt gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag
     3301 aagcaaaagg gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg
     3361 ataatttaat tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa
     3421 tggcaaaata atcactccta acattgagaa taaataaaag aataacattt tttctaaaat
     3481 aagcaaataa aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa
     3541 caatattatt ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga
     3601 aaagtaacaa aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac
     3661 ggctcataac cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa
     3721 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact
     3781 tggctgttct tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga
     3841 aatcagcgtt ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg
     3901 ccgtttgtta agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt
     3961 gagcctgccg tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg
     4021 taggactaaa acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat
     4081 agatattttt atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat
     4141 tctccgtgtt ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa
     4201 gcactctgca ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact
     4261 tcgatctctt tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt
     4321 tcaggtggaa tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga
     4381 agagggatca attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata
     4441 tcccgtgtga agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata
     4501 cgttctgcca attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga
     4561 ttttgcatat caataataac taacattttc tttcctccta cattcatatc gtcattttat
     4621 tctttttaat agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt
     4681 acttggtttg caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg
     4741 ctgtaattca acatatcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat
     4801 tcgattccaa ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc
     4861 gtgatttcta agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct
     4921 ctttgtagct tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta
     4981 agagaaggat cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa
     5041 tgttcataat aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct
     5101 tgggtttctc ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt
     5161 ctcagtgtat cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc
     5221 atttcagcaa tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa
     5281 aaaatatctt tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt
     5341 tcatcaaatt tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc
     5401 aaggtaccgt ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca
     5461 tatctatttt cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc
     5521 tttgtcatta tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag
     5581 tttttggtca atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat
     5641 gcgttttgtg ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt
     5701 aaaaccaaat gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag
     5761 tgattttgat ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt
     5821 atgctcctta cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac
     5881 gcataaaatt ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag
     5941 aaacggtaaa taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata
     6001 tctacgaaga cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt
     6061 taccaaactt attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt
     6121 tagtactaga cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg
     6181 ctattatcga tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact
     6241 tgtacttcaa ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta
     6301 cagaaaaaac tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag
     6361 atgctttgaa tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc
     6421 agacttcgct gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata
     6481 aagaaggaaa tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata
     6541 cgttgaaaga tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa
     6601 cgaaagtagg cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca
     6661 aaattcgtac cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct
     6721 aaatttgttc ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta
     6781 cgcttctcag tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa
     6841 gaattcttta caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt
     6901 gcgattccgg aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac
     6961 atgatcgctt tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta
     7021 gcagctgttg aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa
     7081 tcaatgcgtt atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa
     7141 aatattacgc aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat
     7201 gaccaagatg cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg
     7261 aatgcacaag gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt
     7321 gaatacgaag aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg
     7381 tggagcaagg attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg
     7441 gctttccgtt gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa
     7501 acagatgtag ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa
     7561 ttacaatgtc taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa
     7621 tcgcactcgc gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa
     7681 aaggcagctt ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa
     7741 tcgagcatcc attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac
     7801 gattgaacat ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag
     7861 tactcgatta taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc
     7921 caaaaaggag gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa
     7981 cagatgaaaa aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc
     8041 aagcaatcga agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag
     8101 aagtttctcg gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag
     8161 tgattaatct agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg
     8221 tactttccga taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc
     8281 tgctgacaaa gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat
     8341 tagatattta cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt
     8401 tggataaagc agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg
     8461 aaagagacat cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat
     8521 tatggaattc tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc
     8581 atttgagaca gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta
     8641 ttggtgatct attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag
     8701 attcagctgc attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata
     8761 caggaggatt accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt
     8821 tgatagcagc agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa
     8881 tctatttaaa acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag
     8941 gaggaataca gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga
     9001 gaaatttcct aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc
     9061 tgattgtgga gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat
     9121 cactggtgga gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc
     9181 agatatcgct cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata
     9241 cgtgaaagtt gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc
     9301 acctaaagaa atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact
     9361 aaacgcagga cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa
     9421 agacacattt atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca
     9481 tatcccagca ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga
     9541 acatttatta gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc
     9601 aaaacagctt gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac
     9661 accattacaa ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag
     9721 tttactagaa cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc
     9781 gaataaagca agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa
     9841 cttataagaa gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa
     9901 aaacgatcgg agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat
     9961 taagtgcaac ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta
    10021 attcatacgt gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta
    10081 tcacgttact tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg
    10141 gtgatgggga gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc
    10201 atgaagatga attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg
    10261 ccgtcaatgt aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat
    10321 tgatgaaaga aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag
    10381 tatcggtgaa atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag
    10441 tagaataggg ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc
    10501 tttggggtac ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc
    10561 ttattgatct tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg
    10621 aaaatcttag atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa
    10681 aataataaca ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca
    10741 ccaaaccaat caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat
    10801 ccagatccag tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa
    10861 aagcatgcac ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca
    10921 atcgatcctt ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa
    10981 gaaaaactta gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat
    11041 aaccaaggaa agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa
    11101 gataaagttg tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct
    11161 gcaaacggtg gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg
    11221 gctgggatcg aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg
    11281 gaatatggca aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa
    11341 gtaaaaaata tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa
    11401 acaaaagaag aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg
    11461 aaaaaaaccg gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc
    11521 ggcgcacaaa aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca
    11581 ttaggaggaa cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc
    11641 aattgcagga gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc
    11701 aggtttagtt ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc
    11761 aggaatggca atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt
    11821 ctttgctcga aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat
    11881 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata
    11941 ttatcgaaga tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact
    12001 tttgtcacag cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc
    12061 ttaaatgtgt ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt
    12121 ttcttgagat aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga
    12181 tccacaaatg gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga
    12241 gattgaaaaa gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat
    12301 aatagctcgt aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag
    12361 caatcgtatc atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa
    12421 tatagatcac gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa
    12481 ttgtgtgagt tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc
    12541 gtaacaaatg ttatctaatg tatttttaaa taaataatca ttattatcaa aataatg
//
LOCUS       DAFFFQ010000086        11930 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000086
VERSION     DAFFFQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.087"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            146..583
                     /locus_tag="LQB69_002442"
     CDS             146..583
                     /locus_tag="LQB69_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526575.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            678..1562
                     /locus_tag="LQB69_002443"
     CDS             678..1562
                     /locus_tag="LQB69_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL3526576.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1786..4953
                     /locus_tag="LQB69_002444"
     CDS             1786..4953
                     /locus_tag="LQB69_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526577.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4973..5518
                     /locus_tag="LQB69_002445"
     CDS             4973..5518
                     /locus_tag="LQB69_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HBL3526578.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5546..6001
                     /locus_tag="LQB69_002446"
     CDS             5546..6001
                     /locus_tag="LQB69_002446"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HBL3526579.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5998..6756
                     /locus_tag="LQB69_002447"
     CDS             5998..6756
                     /locus_tag="LQB69_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL3526580.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6842..7582)
                     /locus_tag="LQB69_002448"
     CDS             complement(6842..7582)
                     /locus_tag="LQB69_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526581.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7643..8896
                     /locus_tag="LQB69_002449"
     CDS             7643..8896
                     /locus_tag="LQB69_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL3526582.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9218..9445
                     /locus_tag="LQB69_002450"
     CDS             9218..9445
                     /locus_tag="LQB69_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL3526583.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9568..9894
                     /locus_tag="LQB69_002451"
     CDS             9568..9894
                     /locus_tag="LQB69_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL3526584.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9962..10675
                     /locus_tag="LQB69_002452"
     CDS             9962..10675
                     /locus_tag="LQB69_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL3526585.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10985..11836
                     /locus_tag="LQB69_002453"
     CDS             10985..11836
                     /locus_tag="LQB69_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3526586.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         4066 a         1988 c         2491 g         3385 t
ORIGIN      
        1 aatcaacagt agtttaatta ctacttgatt tagttctttt tattttaaaa atttttttta
       61 cttagaatcc agcgacccta gatatttttc tgtataatct taaataaaga aaacaatatc
      121 aaaaaatgca cgtagaagta gataaatgga gtggttttgc aaatttcttt tatttctaga
      181 agttattcac gtatacggat ttgtttttct cacaaataaa aaggcaggaa gtacaatgga
      241 tcatttgtat ataaaatata ttcatcaatt ttccgttttg gtgttaggca cactaattat
      301 agcggggatt gtcgcattta ttattcaagg ccgtgacata ttaacagtac atcaagagtt
      361 tttcagttat gcatttggta ttcctctgat cgctataagg tctgataaca aaaagtggaa
      421 gaaacgctat tacattctct tgctattttt gtgtttttat ctgccaccta agatttttac
      481 tttattaggt atacaatgcc tatcattttc ttttatttcc tatatcattg ttttaatttt
      541 cctcagtttc atagtatgga tgttttatct tgtcaaaaaa taaatgaatg tttcatgaac
      601 agtacttcat cacagcctaa tttttttctt tatgctatcg ttatattaaa gaaatcaatc
      661 agaagggaaa gaaagtgatg aaaaaagttt tattagtggt aaaccccagt tcaggcggtg
      721 aacaagcaaa ggaatttgag caattagcga tagcaaaatt ggaatcggtg tttgatgaag
      781 tggtcgtctt gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta
      841 cggaagggta tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac gaaggaatca
      901 gtggaatcgc tgaacaagaa catcggccaa atttcggatt tttcccattg ggtacggtga
      961 atgacttggc tcgagcatta gggataccgc tagaacctga ggaagcgatc aatcattttt
     1021 cgattgaatc agtcaagcca ttggatattg gaaaaataaa tgacgactat tttatgaatg
     1081 tcgtcgcaat tggctctatc cctgaatcaa tcaatgatgt agatgcggag aagaagacga
     1141 aatttggaaa actagcttat ttcatgtcag gaatcaaaca gttagcttcc acacaatcct
     1201 actcgtttca cgtagaagtt gatggaaaga aggaagaaat tgagagcagt accttgctga
     1261 taggactaac taactcagtc ggcggttttg aaacattact tccaaatgca aaagtggatg
     1321 atggtaagct ccatttggtt tatttaaaag attcttcttt gatcgatacg cttaaaacat
     1381 tgccagacct attaaaagga gtagatgaat ctacgaaaaa tctagcctac cttacttgtg
     1441 agcaattagt tgtgtcatta gctgatgagg aagaattgac gacgaatgtt gatggagatg
     1501 aaggggataa attacctgtg acaatcaaag tattgccctc acatttacag gtttattgtt
     1561 aaaatggagg ttgtgaccga ggagttggcg ggagaacacc gcttgttcgg gataagggac
     1621 tgtgacaaga cttttgtcac agcctttttt ttgtacaaaa aagaaattta cacctaatag
     1681 aaaaattttt ccataaaaat acatatttta aatatgaaag tacaattgga aatagattaa
     1741 aacgataaaa taaaattgtc aaaacgtcag agaggatggt ggagaatgaa tcaatgtgta
     1801 aaaaagctga ttgcgtcgat tttgtttgtc ttccctctct tgatggtgac atccgttttt
     1861 gccgatgtag agaaaacaaa cgaatcaacg caatcagaga atcaaagttt tgacaagact
     1921 actttttcag aagaatcgat tacttctgaa actacagaat ctcaaagtga ggcttctact
     1981 aataaaaggc aagaagaaaa taagacaagt gagacctcag aagtagctga acaaacgtct
     2041 gatagcagtg aagcggcact ggcaagagct gtttcagcgg atgggtacag cgaagctgcc
     2101 acaacagaag aactggcgca gcttctcgca gacgaatcag taactaagat ccgactgatt
     2161 cagcctttga ctttagacag ggaattagag atcaaacgag atatcgttat tgattttggt
     2221 ggattcgctc acaatttcgg cacacaccat atttatatca atgagatacc tcagtcgatc
     2281 gagttccaga attttaaagg aacagctgca catccaggtg gattgacacc aaatatcgat
     2341 ggaaatgcca ttatcattgc ttatatgacc gatttttggg gaagcagata tcattttacg
     2401 ggagaaataa aatttaccgg gacacttgat ttgtatgatg ggtccaaatt aggactgatt
     2461 tacgctcctc gtgcaactgt cacattagat ggtgtttccg gtgtgttgga tgtccaacca
     2521 gtgaaggaag gactgaatgc cgctccgggt aaagcctatt tttgtagaag ctatcaattg
     2581 aatgtgatca atggtagtca actttatgga ccgtatctag ggaaattcta cggttttcta
     2641 gattcaggag atgagactgg tagtggaaaa acagcgccag gaatccatat cttaagcggt
     2701 tcaaaggtct ctcttgatta tgatcgggca gatggcgtta gcgatgaagg agaggcagtc
     2761 gatacgctac ctggtaatgt catgtttgaa gtcagtggtt cgggaagcga attttcggta
     2821 aatacaaaga tcaatattac caatgacaac aatcgcggaa tcatccaaat gagaggttca
     2881 ggatcgaaag tcactgtatc gaatggtggg aaaatcacca tcaatacagc aactacgggc
     2941 ggatttcgct tgcaaggaga ttctagtcaa atccatgtat caagtggtgg acaaatctcg
     3001 gtcaaaatgg ctggagatga cgatcaaccc gggaacaatg ggatgcgttt tgtaggaaaa
     3061 ggcctttctt tgatcgtaga aggagccgga tcagttatcg acatcgataa gcagagtggg
     3121 cgttccagcg cagttcgttt tgagaatggt acgcaaaagc tgacagtaac caatggaggc
     3181 tcattgaggg taagaaatac aggtgatggc aaatcttacg tgaacagact caatcaagga
     3241 aatcaagcga tccaatttta tgacgcttct ggttttctag aaagtccagg ttcagctgat
     3301 tttacagtca ctggagaaaa atcaaatatc gatatacgcg cagattccgg agcaactgta
     3361 gatacaactg gagatattga tttaaatttt acggcaggtg aaaaaactta tttagtcatg
     3421 gtcgggaaaa caggggaaga taagaccggc atcatcagtg gggatgtgtt gaatgtggac
     3481 ttagaatctc ctacctattt tgatttccaa aacaaacgga ttggaagcga gaccaatggc
     3541 ataggcggct gggtattcca aggaaatgga gattcttctt taaagatcgc ccattcagaa
     3601 atcgctttat ggcgaaaaac aggcgcaaca ttcgataact ttgaaaaaga cccggattat
     3661 ttttctgata tgacagactt atcgatcaat gggaccgact taggcaattt cgtatcaagc
     3721 cctagtgcag gtttaacaaa tgaatttgct cagacaggtg ccggaatgaa aaattataat
     3781 cgaatcagtg ccaataatca gctcccgatg attgaatcct taagagtgcc aacgaatgca
     3841 gataaaaaaa tctatgggca tgtcgttgtt cccgaaggtg tcgaggcaaa gccaagagat
     3901 gcttggacag gcgaagtaga agtgacttta cgtattactt acgcagatac gacgaaatca
     3961 tctgtagaaa ttactgctct cacgcaagga caaacagatg aaaatgaagg ggcaggatat
     4021 aacccatggg gagaaggcga acaaggcggc ttgttcgaag ttcaagtacc caatgatgag
     4081 agattaaaaa cggaggatca agtgcaagtt gttgctgcta aaaaagtaag aggtggccaa
     4141 gaagtgacag aactggactc tgccaaaacg acagtcgatg tgattcctcc tgaacctgct
     4201 aatttaagta ccacattgat caatgcagct tctcgcacga tcacaggtac aggtgaagca
     4261 ggagccttgg tttcattgaa aaaaaatcaa gctgttttgt cagaaacaac tattgatgat
     4321 acaggggaat ttgttctttc tatcccagaa aatcaacttt ttgcaggaga tgtattagaa
     4381 attgtattaa acgaccaagc aggagaagct actgctaagg gtgtgcagaa caagccaagt
     4441 accaacgatg aaattgggaa tcacaatcca agtgatacgc cagttgttta tcatgatgcc
     4501 ccgccttttg cacctgctac gaagatcgta gtagaaggtg gtctctcatt tcttgcccct
     4561 tccaaactcg attttggtca gatcaaagca actggtttga cacaagaagc atttggccaa
     4621 atggatatac aggaaaaatt atggatttat gatcagcgag aaacgaaaaa tccgtggatc
     4681 ttaacgataa aattatctca gcctttcaca aatgatgcag gattttctat gcaggatgtc
     4741 ctatatttca agaaaggaga ggagtatcaa ttagtgaatg aagaagaact ggaaattgtc
     4801 agaggacaaa attcaacgaa tgtgcaagca gattattcag aatatttaaa taacggtcgg
     4861 gcgtttaaac ttgtcttaac caaagaaaat cagatagctg gtaattactc agcagagata
     4921 acatggacat tagcagatgc gccgacagaa taaagatcaa ggaggatgat tgatgaatcg
     4981 aatatggaat aaaagtcatc gaaacaactg ctctttactt atgatccttc tccttggatt
     5041 catcctttgg cagacacctg tccatgcctc cgaattcaat tttgcggtag attttgaatt
     5101 accggctaat cagattgata agaacaaaac atatataaat gtaaggatgg agcctgggaa
     5161 aacacaggaa atcccctaca cggtctttaa ccgaacagat aaatcagtaa cagttgagac
     5221 attgatccac tccgctacga cgaattcaaa cggtgtgatc gaatatggag aatcaaaaag
     5281 taaatttgat gaaacattaa gttatgatat gaaaaactta gcaaaaacag tgggagaaat
     5341 aactgttcct cctcaaagtg cggtcactga gacactttca ctgaaagcgc cagaaaaaga
     5401 gctggctggt gtggtcgcag gagggatcac gtttcaagaa aaaaaagaga acgtttctaa
     5461 caaaagtaca gttggtgtcg tcaatcagtt ttctcaagtg aaagcaatct tgatttgaaa
     5521 tagcgaaaaa gagtcttccc cagatttgaa attaaaaaaa gcttatgcgg atcaaagcga
     5581 tggaagaaat gtgattaagg cgaatcttca aaatccaaat gcttcttata tgttcggctt
     5641 gactacatcg gttgaaatct acaaaaaaga aacaaaagag ccttaccttt caataaaaaa
     5701 agaaaaaata gatgtagcac ccaatacaaa ttttgattta cttgtcccat tagaaggaaa
     5761 aaaattagct ccgggacatt atcaagcggt tgtgaagggt gcttggaaag aaaaacgctg
     5821 gaatctccat accgattttg aaataactga tgatgcagcg gaccaattga atgaaaagga
     5881 tattgattta aaaaatcagc cagaaacatt tgactggcga tggctgatac ttgttggagt
     5941 cattcttatt ggcttatttg ctggattatt tattttaaaa gaaagaagga aaaagaaatg
     6001 aaaaaaacaa ttgcattaag taccgttcta gctggaagtc tattattatg tacacaaagt
     6061 gtttttgctg agaataagag ctcagccact acagaaggaa cgatttctgt gaccaaacca
     6121 gatgctgcaa atcctgaaga cgttgttcca gttcctcctg ttgatccaaa tgatcctgat
     6181 actcctttta cacctgacga accaaatcag ggaacatccg gattactgac gattgaccaa
     6241 gctccagatc ttgattttgg aacaatcaag ttaggtacag caaaaacagt atatgctgca
     6301 ttacaaagcg gaacggacag tactggagca caaacacaag tacagaattt cgtacaagta
     6361 acggataaaa gcggaaatta tgccggttgg aaattatcag tcaaacgaac agaatttatt
     6421 ggtacaacat ctgatacgaa taaactaact ggttcaaagg tgaaattcaa aaacgccgtg
     6481 attcgaaata gcgcaactaa tagcagtgga atgccatcta ccgtttctgg aactggcgct
     6541 gaaggaatcg aaatcccagc agatcaggca gtagatctag taactgcagg agcaaatgaa
     6601 gggattggca catggattta ttcattagga gagaatgtgg aacaagggag acaatcagtg
     6661 gaattatttg ttcctgtatc caattatgct gtagacaact acgtttcaac attcacatgg
     6721 tcgttaacag atgctcctac agaagaggaa gcgtaaaatc atatcaacga tttcagggag
     6781 tggagccgat tcaaggccca ctccctgaaa tctgctatgc ttacgtctca gctcttattc
     6841 atcaactcga agcagttttt tggaagtcat ggaaacgttc ctttgtcttt tcaaagatct
     6901 ttcgaccaac gagtaaggag tcaccgttga aaaattctaa cacataatga gcagaatcaa
     6961 ttttctgcaa ttgcgataag ttgatacagt aagattttaa aagtaaaaag taagcatact
     7021 tttctaactc tgtcttcaat tgttttaaag aagtattcac taatttatct tctgtagttg
     7081 ttttgaccat aacttggttt ttcaaacctt tgatactttc aatatataaa atttcccgag
     7141 gatacaggac gagacttcca cctttttgat caataaagat tttatcgaca tttacgatct
     7201 gctgttcttt ccaattgatc tgttctagca cttgacgaat ttgttcagat aaattcctgt
     7261 cttttctgat atatccaatc ggaaggattt tccgattgat gatctctact gcaaaattcg
     7321 tataagcggt gaaaaaaaca atattactat gtgcattttt tttacgtatt tgttcggcga
     7381 catcaatacc tgtgagtgat tgcttcaagt cgatatcgat aaagaaaaca tcgtgattat
     7441 caatagtgcc caactggatc aactgattga tttgagagat agaagtgagc tgcacgatat
     7501 tctcgatatg atgattgacc aattctctga tgatccattc gctgtgaaac tggttatctt
     7561 ctatgataaa atactttttc atatgcaggc tcctattaaa aaaatatgtg tacattttga
     7621 aacaaaacaa tggaggaata agatggtcgc aacagtgaca ctttatttga cctttacttt
     7681 gattttagat ttaggtatct atttattctt ttcgaaacaa ctgctagtaa aaaacagctt
     7741 tggttgtata gctttgatgg tggtgacact tttacttcct atccctaaag tgagctttcc
     7801 ttacctagtt ggcttattat tgatgtttct tttttatttt actacgaaaa acctgtttat
     7861 cagctgtttg atcagtaatc ttttttttgc tatcactgga ctcagctttt ttctggtttt
     7921 tgatctgatg gattatttag gaaagagaca tcttggaaca gcaagttttt ttgcactact
     7981 tatcgcattt attttctatc tattagtaaa aagtatagac aaacagacga gatttctcaa
     8041 cttgcttttt acttatccaa ctgaacacta ttttggaggt atttgtattt tattaagctg
     8101 gggcgtgatt tacttggtat tgggaagtgt tcacttccgc tcatctaatt atctgcttct
     8161 atctcttttg tcgatagtca tcagtttttt ttctttgttt ttttccatta tgttgatcag
     8221 tttccaagta aaagaaagac aacatcagga atcgcttcaa ctgtatcaaa taaatgaaga
     8281 atattaccat catctagagg aattcaaaca tgattataaa agtctactgt tttcattaaa
     8341 aattactctt caggaaaata aaaaagaaac gttggaattt ttagaaatat tagaagatca
     8401 ttctgcagct atttttgaaa aaccgcaaat acaacagcta acaaatattg tatccccagc
     8461 tgtcaggggt gtctttttaa aatttctaga aaaagcagaa tcggagaaaa tcccgatcca
     8521 agtagttatt cctgtcgaag tgaaaacaat cccgattgat ctggttgttt ttattcgctg
     8581 tttatctatt tttatttcta acgcattcga tcatcgtgta gccgatcaga gtccgattac
     8641 gatcaatctg ctggaagaaa aagagggatc acgttttagt atagcgaaca aggcaaatcc
     8701 aacgaatttg acgctatcgg agatggtcaa aagaggaaaa acctctaaaa aaaatggcgg
     8761 gttaggtcta tacagtgcga aaaaaatgtt ggatgtttac gaaaatgcag aactaaaaat
     8821 cgaattttca aaacagaatc actatttttt tgtagaaatg tatctggcat ctagaaagag
     8881 caaatcagcc tactgactta ctgtcaaagg ataagtttga ggaaagtcta cacggagata
     8941 gagacaacaa cacagcatat tcaaaagaaa cagaagcgtg atgaccgttg tcacccttct
     9001 gtttcttttg ctatgtagtt aaacaaagta gttgacactt ccgctactta gtattagtac
     9061 gaaagtatac ttcatgatac taagtagata agaagtgaaa agcggaaccc acgatcgtca
     9121 aaatgctaac tgctttgctt acaccagata aagggaagag caatatcgct tattttaggc
     9181 gtggagaagc aagcaaatcc ctacgatcac aaaaaaaatg ctgacccagc agttaagaga
     9241 gttagaacaa gatgaaagta tccatcgaaa agcctataac caagtcccgc ctaaagtaga
     9301 gtactcgctc accgaagaag gaaaatcatt aagagagatt ctagtagcgt tgtctgtttg
     9361 ggaagaagaa cgtatcgaaa aacaaaaagc ctcaagggaa gaagacacgc tactgcatac
     9421 acatggtggc tacctaaatt attgattttc tatgacattt attttaaata aaagaaaaca
     9481 ggagtttttt taaaagcatc atctattttt tatgtaaaaa aatggtatga ttaatcaagt
     9541 aagaagtgat tggaagaagg caagcaaatg aatagtatgg aaaaagtcaa ccagtttaga
     9601 gatgaacgta attggcgaca atttcataac gaaaaagatt tggctatctc tatttctttg
     9661 gaagcatctg aattgttaga actttttcaa tggaaaacgc cggaagaagt aaaagaaaca
     9721 caattagaac gaatcaaaga agaactggca gatgtattga tctattcata catgatggca
     9781 gacaaccttc acctggatct agatgagatc attgaagaaa aattgatcaa aaataatttg
     9841 aaatatcctg tagaaaaaag cgcaggtgta cgaaaaaaat acacagagtt gtagaaatgt
     9901 gtcattcggc tgttggaaag agcaaaagaa aaacagtaaa atgagtctgg agggagagaa
     9961 aatgaaaaaa aaaggaaaag caaaaagatg gctagtcaat atcctcttgt tcctcttgtt
    10021 attagtagga ctagctttga ttttcaatga acagatcaag gattattttg tcagagagac
    10081 aggagataaa tatgctatag cgaatgtaac aaaagaagat ctgaaaaaga ataacgataa
    10141 agatgtcagt tttgactttg atgcggtaga accaatgacg acagaagggg tcatgaggtc
    10201 gcaaatgaga ggaacagatc taccagtgat tgctagtatc gcagtccctt cagtttcaat
    10261 caatttaccg gttttcaaag gcttggataa tacgtcacta ctttacggag caggaacatt
    10321 atctccagat caagaaatgg gaaaaggaaa ttatgcctta gcaagccatc gtgcaacgaa
    10381 ccctgaattg ctgtttacac cgctagaaaa tttagagatg ggtgccaaga tctatttgac
    10441 agatttagaa aatgtgtata catacaaaac attttttaaa gaaaaagttg ctcctacaga
    10501 tacccaatta ctgaatgaag tcgagggaaa agaaattgtc acactaatta cttgtggcga
    10561 catggacgcc gtcacgcgac tagttgtaca aggagaactg gaaagtgtta catcgataaa
    10621 agatgcgaca gatgatatga gatctgcttt caatctagag acaaaaacat tttaggatac
    10681 tgggacataa gtcgttacag attaaaaaca gccaaaggat atgaatcgat aaggaaaaac
    10741 caatttgctg aaaaaaagag aaacgcaaag aggtgcatct ctgatttggt tgttttctag
    10801 atagacttgt gttccagttt ttttattttt tatatgaaaa ggcttgcata aataaaaatt
    10861 cgatggtatg atgattttga ataagaaatg gattgttact gtacggcagg caaaaccaga
    10921 attcagctaa gaaacagtag gcgaattcta ggttttttta tttttcttga gagggcgaag
    10981 caaaatgaaa attgataaaa ttttgaataa caatgtcgtc atttcgaaaa atggattcgg
    11041 ggaagaagtc gtttgtatgg gaaaaggact agcttttcaa aaaaagattg gggacgagat
    11101 ttcgccagaa gcggtccaaa aagaatttgt tttaagagat tcttttgcta aaaaccaatt
    11161 ccagcagctg atggctgata ttccggcaga ggagatcgaa tgggtcaaac aagtagtcga
    11221 actggctgaa gataaactaa aagttgaatt ttcgcctaat atttatctga cattgaccga
    11281 tcatcttcat tatgcgatca cacgagcaaa agaaaatatc caactgccta atccattatt
    11341 atttgaaaca aaaaaatttt atccgaaaga atatcaagca gcgaaagagg ctcttttgtt
    11401 cgtgaaacaa aagacgggtg tggaactttc tgaggatgaa gccggtttta ttgcctttca
    11461 ttttgtgaat agccaacaag gaaacgaaga catgcaagtg acgatgaccg ctaccacaat
    11521 ggtaagagat atactgagta tcatcagtaa attcttcggc atcgtgttcg atgaagaatc
    11581 tttgaattat cagcgtatca ttactcatct gcagtttttt gcccagcgtt atctcaaagg
    11641 ggaaggatct gacgagcaag atgaatttct ctacgcatta gttcaaggaa agtatccaaa
    11701 agcgtttcat tgtgtcgaac ggatcaatga gtatcttcga caaacgcaaa aacaggaaat
    11761 ggggatcgca gagcagattt atttaacgat tcatattcaa agagtagtaa gtgagaaaaa
    11821 aagcatccaa tcataaggat tgttactgta tggcaggcaa aacctggatt gaaaaaatag
    11881 gttcacgaat gtttgtgact gtttttttca atctgggttt ttatttttta
//
LOCUS       DAFFFQ010000087        11440 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000087
VERSION     DAFFFQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.088"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            184..1182
                     /locus_tag="LQB69_002454"
     CDS             184..1182
                     /locus_tag="LQB69_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3526587.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1294..1803
                     /locus_tag="LQB69_002455"
     CDS             1294..1803
                     /locus_tag="LQB69_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL3526588.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1833..2492)
                     /locus_tag="LQB69_002456"
     CDS             complement(1833..2492)
                     /locus_tag="LQB69_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL3526589.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2494..3258)
                     /locus_tag="LQB69_002457"
     CDS             complement(2494..3258)
                     /locus_tag="LQB69_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL3526590.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3266..3943)
                     /locus_tag="LQB69_002458"
     CDS             complement(3266..3943)
                     /locus_tag="LQB69_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL3526591.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3980..5365)
                     /locus_tag="LQB69_002459"
     CDS             complement(3980..5365)
                     /locus_tag="LQB69_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL3526592.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5575..5659)
                     /gene="ffs"
                     /locus_tag="LQB69_002460"
     ncRNA           complement(5575..5659)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LQB69_002460"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LQB69_002461"
     CDS             complement(5682..6197)
                     /gene="tadA"
                     /locus_tag="LQB69_002461"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL3526593.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6304..7161
                     /locus_tag="LQB69_002462"
     CDS             6304..7161
                     /locus_tag="LQB69_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526594.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7242..7577)
                     /locus_tag="LQB69_002463"
     CDS             complement(7242..7577)
                     /locus_tag="LQB69_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526595.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7644..9014)
                     /locus_tag="LQB69_002464"
     CDS             complement(7644..9014)
                     /locus_tag="LQB69_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL3526596.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9092..9628)
                     /locus_tag="LQB69_002465"
     CDS             complement(9092..9628)
                     /locus_tag="LQB69_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL3526597.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9937..11418
                     /locus_tag="LQB69_002466"
     CDS             9937..11418
                     /locus_tag="LQB69_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526598.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3515 a         2301 c         1854 g         3770 t
ORIGIN      
        1 tttgcatact tcataataat aataacataa tacctaacct ctcgtaaaaa tactagattt
       61 gttttctttt atttttcatc actagataca ttctataact taccgtttcg atttcgaatt
      121 caaacatttt tgagaagcat tctcaattaa attctgctat agtgtgaaaa gggaggaatt
      181 tttatggaaa tctatgacat tacaatcatc ggtgcaggtc ctgtagggat gtttgccgct
      241 ttttacgcag gaatgcgcca agctaaaaca aaaatcattg acagtctacc tcaacttggt
      301 ggtcaattag caactttgta tcccgaaaaa tatatttacg atattcctgg gtatccagca
      361 ataaaagcaa gtgaattaat cgatcaatta gaaaaacaat taactacttt taaccataca
      421 ttccatttaa aagaagaagt tctctcgctt actcgtgaag acgaagttat cgagattact
      481 acaaataaag gcatccatta ttccaaagca gtcattttag ctttaggaaa tggttcattc
      541 cagccaagaa aattgaattt agataatgct gaatcatttg aaaaccacgg attggattat
      601 ttcgtgaatg atttgatgaa gtatgctgga aaaaaagtcg ctattgctgg aggtggtgat
      661 tctgctatcg attggacgtt gatgttagag cctattgcaa gcgaagttta cttgattcat
      721 cgtcgcccgg aatttcgtgc gcacgaacat agtgtatcac gcttgaaatc ttcatctgtc
      781 aacttgctta ctccctatct catcgatgga ttatcaggca ataatgggga actgacagat
      841 atccggttaa aaaaagtgaa aagtgacgag acgattgatt tgatgattga ttctctgatc
      901 gtcaattatg gattctcttc taatttagaa catctttctt cttggggact tgatagtacc
      961 agaaatgcca ttactgtaaa atcagatatg tctacttcta ttccaggcgt ttatgcagct
     1021 ggtgacatct gtacctatga aggaaaagtg aaactgattg ctactgggct cggtgaagca
     1081 cctactgcag tgaacaacgc tttacactat attaatccaa aggaacgaac tcagcctggt
     1141 cacagcacaa gcttatacga taaaaatctt cgtcctagtt gattttttat atctctttgc
     1201 atttcaagtt aaacccgaaa gcagtttctt gcattttttt catgttccgt taacatgaaa
     1261 tagagaaaga aaaaataaag ggggagcaat aatatggtcg tcaaaactga gactttagaa
     1321 gaaaaggcaa gagaactatt gaaggaacgt ggcgtaactg tagaagatat cgcacaactt
     1381 gtcatgtttt tacaaaaaga ctatatttct gatttgactt tggcggattg tactgaaagt
     1441 gtcaacagcg tgctaacaaa gcgtgaagta cataacgcca ttatcactgg aatccaatta
     1501 gatatattag cagaaaagaa aaaattgatg agtccgttgc aacatattat cgaagatgac
     1561 gaaggccttt acggtatcga tgagatccta gccttgtcaa tcgtgaatgt ttacggtacg
     1621 atcggcttta cgaattatgg ttatatcgac aaggtgaaac ctggtatttt aaaagaacta
     1681 aacagccacg atggcgttca tgtccatacc ttcttagatg atatcgtcgg agcgatcgcc
     1741 gctgctgctg ccagtcgatt agcacacaaa gaaccagaaa aaagaagtcg ccttactcaa
     1801 taattcaaca aaaaaagaaa gaaaacgtct atttagacga gttctttctt ttttgtttgt
     1861 ttcaagctat tttttcctaa gaaaagaaaa acagcaaaaa ataattctaa taatacaaaa
     1921 aataagataa agctatgcat gaaaaattct tctggaagta cattgatgat tggatcagta
     1981 acgggatcga ataaccagtc atcgttgttg aaaaacgttt catgaaaaag gataaaaaag
     2041 cgatcaaatc ctatcatcat gaaaaaaccg aaaacaactg gcagtaacat gccgatttga
     2101 aacggacgaa tcaaccgcca aagccgatca ttcttttgca aatatttgat aaacaaaata
     2161 ctcgggatca aagtgatgaa aaacacagca taatccacta aaaacaaaat tttcacttca
     2221 taaaagtgat gggctccgct agcagaaact gggaaatctg gcaagctgag gatggttttg
     2281 aagggattat tgagataatt cattaggtga tcaaaatttt tcagtaacgt ctcttgatct
     2341 aaagaagtat aatccaaaat ttgcaaatgt ccgatatcaa atacgtatag tggtcgaaag
     2401 ttgatcgtca caccaactgc cagactgatc agtgtcagaa ataggctgaa caatccagca
     2461 tattccatcc actgccatct cgaatttttc atattagaag ctccattcat ccagtgattg
     2521 tttcacatag gtagctggga taggcaaccc aggtacatct tcttcttttg taaaacctga
     2581 taaaacgagc aaagtatcta tattattgcg tatcccagct tggatatctg tttcatagtt
     2641 atctccaacc atgattactt cttctttttg cagccctaat acttcaacgg ctttatccat
     2701 aatgatggct tctggttttc cgatgtagat cggcggcgtt tgggttgctg tctctacaaa
     2761 agagatgact gaccctgctc caggaagaag gccgcgttca gtaggtatat tcttatctgg
     2821 gttcgttcca atgaaagtag cacctttttg aatagcaagt gttgctttaa ctactttttc
     2881 ataggtcaga taattatcta agcctaccac tacatagtca ggagcctctt cttcccatgt
     2941 aaatccggca gctaaaatca agtcaatcaa tcctgcttct ccgataacat agactttttt
     3001 cccttttcca tctgctttca taaaatcgat tgttgccaaa gtagctgtgt agactgtctc
     3061 tggagctaca tgaatgtcaa actcatctgc tagacgatta gctactgttt ctggtgactt
     3121 agtcgtgtta ttggtaacaa acaagaaagg taattttctt ttttgcaatt cttccacaaa
     3181 tcgtttaccg gccggtatgg gttgtgtccc acgataaatc gttccgtcta aatcaattaa
     3241 atatccttta tagtccattc atccactact cttccctttt tctgatcgta aatgctgttt
     3301 ttgttttttc tacttgttta ggttgttctt gtttttttac tttttcagag gtttttgctg
     3361 actctgtttt tcgattttta ataaccggtt ttttgcgatt agatgcgact ggtccttttt
     3421 tctcatcgat atacgcacga ttatggtttt ttttcttttt tcggcgctga gttgtctttt
     3481 cttttttacc gccgattctc tcgatgacaa aatacgcaca accaaaattg caatattcgt
     3541 aaagatagtc ttccaaggaa tcgattcgct gatctggatg cgcccgtcga ttatccgctt
     3601 ggaaaaatcc ttttaggcgt agttgttcat aaccccaatc tcctacgata taatcatatc
     3661 gggctagtac atcactgtaa cgttctccaa gcttttctgc attaaatcct tcacgataat
     3721 cggacacaag ccgataatgt ctttcaccta tcaaaaaatt actgtcatct aacagtgtca
     3781 ctagctcgcc tttgactttt tctgctttag gctcctcaac gatttcttcc aaagcagctg
     3841 tcagttcttc ggtgactgtt gtttcctgtt cagctgatga ttttttctta gcaatttctg
     3901 ttttatgctc aacgttctgc tgttcatttt gttttgcaga catgcgttca ccacctatct
     3961 tttttattat accttgtttt tatttgattg ggtagtgagc tttcaagtaa tctcctacca
     4021 cacttcggat aaattcaggg aacaaaaatt cattttttcc agcaatctca atcgttggaa
     4081 aaaatggaac aaacatcaaa tgatctacgg tagtaaaagt ataattttta ttcagatcga
     4141 ttggcttgtt cttccaaaag acttgatggt taatagaatc atatgtgatt ccattataga
     4201 ccatctgccc aaaaatctta ccgcgaaatc ccattccctt cataggatag ttacgtaaaa
     4261 agttacgatt tttttctatt tctaaaacta agcggatcag atcatttcca cccaatgtga
     4321 catttaacaa gtgcatcgga tgcggcaacg cagtgtgaag ctgatcctga ttgacccgcc
     4381 cttttggaat ttcagttaaa aacagaccac tgtttaatat tgctgcttcc gtctttcctc
     4441 gttctttcac cgcctcaaag gctacttgga taaacgaatg ctcattgaag atatctagac
     4501 tcagagaaaa tggcaaatct gctacttctt tttcttttaa taattcatgc ccacgttcta
     4561 gataactatt tatttcttgt tcatcttctg taaaagctgt cattgtttcg gtagggatgg
     4621 ctttggctga atgcttaagg atttttttct cttcatcgat ggttaaatgg acttcaccga
     4681 catactgccc gtactttcca gcggctgcta tttggacatc attgattacg tgaccttctc
     4741 tgaataaatg atgggtgtgc gatcctaaaa taacatcaat tgctggtatt tcttttgcaa
     4801 tttggaaatc atcctgaatc ccgagatgac tcatcaaaac taaaacatcc acttgtgaac
     4861 gtaaatcttc gactaattct ggcaaaatat cataaggatt ccgtatgtcc cagccattcg
     4921 gattataagt aagcggaaat ggagcagtaa atgcaattag tccgattttc gtctggtgtt
     4981 tcgttgtaat gattttatat ggtttcgtcc atgccggtgg ctggagcgta tttttatcga
     5041 ataagttatc caggatgata tcaaactcag catgatcgta taaatgattc aactcttctt
     5101 tggaatttcc tacgccttca ttgtttccta tcgtcgctgc atcataacca acttcattca
     5161 tcagcaagat attttcctgt ccattcgttg cttctgacaa tggatgccaa cgatccacaa
     5221 aatcacctag atctactgca atcgttgtcg ttttttcttt cttttcgttt tctcgtttac
     5281 gttgatccaa aaaccttctg atccttggcc aattttctaa atgagaatgc agatcattcg
     5341 tatgtaaaat cactattttt tccatgtacc ctctcgcctc tctttatctt tatcatacca
     5401 taggcagctt tttcttccat tgaaacaagc tctatttcag agtttttcct gccttgaagc
     5461 aaattgtata agtacaatat tcgataaatc agtctggcta aaaaatcagg aacattcatt
     5521 tttacagtac tataaaaata aaaaaaacag acccgaaagt ctgttgtcgt ttcattataa
     5581 tcagacacat ggcggaacct gcttagtgct gcttccttcc agacctgaca cgcttcacaa
     5641 gcccaccatt gtcctagcct ttcggcaaag aatagtatac cttatttttc attcgttgac
     5701 cagtcttttt ttaatttctt ttttcgctgg cggagctttc ggaaaaagtc agtcaaaatc
     5761 atcccacatt cgtcttctaa aatgccacct tctacatatg cttggtggtt aaaccgctcg
     5821 tcctccaaaa gattcatcaa ggtaccggct gttccgcctt ttggatcata cgcgccgaaa
     5881 taaacttctg ctacacgaga caaaatcatc gcgccgctgc acataggaca aggctctaag
     5941 gtaacaaaaa gtgcagcatc ttccaagcgc cagcttccca ctttttcaca ggcttccttg
     6001 attgcaagca tttctgcatg tgtagtagca tcttgcgtgg tttctctaag gttatatcct
     6061 cttccaatca cttttccatc caatacaacc actgctccaa tcggaacttc atgaagcatt
     6121 tctgctttct ttgcttctgc caatgcaagg cgcatccatt tttctttttc tactgcatca
     6181 agatttgtat ttaccaatta cccttccctg cctttgtttt ttcttgtttt actttagcat
     6241 atgataaaaa ggctgtaact aacttttttt tctatggtaa aataaataca agaggtgaac
     6301 aagatgaaaa tggataaatt acttgctctt tacccacaag cacagaaaaa aaactctcca
     6361 gcatcagaag cggatatcct ttcttttgca attgatgatg cttttatctg gatcaaacaa
     6421 gattcattat ctcaacaaga aaccagcctt ttgaaagcgt tattccctgt aatcaacgat
     6481 caaaaaaaac acccatggta tcgctatctt tttaaagatg cttcttgtgt tcaagaaaaa
     6541 agctttcgtt tgatccagct tcacgtcgaa tcaaaaggag aattcttaaa aaaagaatgg
     6601 caagatacta ttttggaatt atttcgctat tctgctgatt tcttttttta tacagacaca
     6661 gatgcgctct tagtcgaaca aataaaccgt gattatcttg atgctactga aatcaacggt
     6721 atttttctat ctttagatgc tgattttgat acgacaacgt ctgtattcgt tggttctttc
     6781 cattcacctg gcaccgacct cgtccctttg tttgctgaag aaagaaaaat ttttttaaca
     6841 gagcaaaaca atctttatac tcacagccgt actttttcta tgcaagatgt ggctttacat
     6901 tactatacaa aagatactgt aaaagatagt aagttgatca aaagttacat agaactgatc
     6961 aaacaaaatg aaatggaagt gatcattcaa gaattatgga atgaattagg caatgtcagc
     7021 tctgctgcaa aaacactgtt tcttcatcgt aatactctaa aatatcgaat cgaaaaattt
     7081 caagaacagt ctggtttcaa tctaaaaaaa gccaacgatt tactgttttg ctatctttta
     7141 ttgcttcatg aacataatta aagatagatt aaaggggttg tgacaataat tttgtcacaa
     7201 ccccttattt cgaatgaata gtgttcaaaa gctagtttct cttacatcat tttttcaaca
     7261 aaatgcatta gccggtcttt tacatcttcg gttgaatctt tgattgaatc accataaaca
     7321 gagattttct tttttcctga tttgattttt gctaacattc ccatcaaaga atccaagtca
     7381 ctatctgaaa gaccatctac gatttctagt aatttttcat ttccatcaaa tttgtcatag
     7441 acgaattttt ttactttcgt ccgagtagtc atatgatgga tcttttccat tacttttccc
     7501 gatacaacga ctgctgtcgc tacactggca gcggctgcta cgcttaatcc gattgttact
     7561 tttgttgatg ttttcataag tatcagctcc tttttatcta tctccatcat agcatagaca
     7621 aaaaaataaa cgaactaatc cactcagtga aatctcaatc ctttagggaa aaagttcttt
     7681 actgttcctt ttacttgttt cccaaatccg acaggtatct tatctaatac tagcaaaacc
     7741 catccttgat tcccatcacg ttgaaatgtt tctcctgctg tgtaagattg ccattccttt
     7801 tgagtgatcg gcaagcaagg tatattctct atcttttttg tagcaagcgc taatgcatag
     7861 ctaggttcaa atcgattctt tttgaaatct cctaaatgca aacctgtacg tacgactttt
     7921 aaaccatcga gagaaggggc taactcagga acttcccaaa gatggtcgtt gaacactaaa
     7981 agtcttccag tagcttcata atgaaaatca tttgaaaact ccgtccagag tttttcctgc
     8041 tcttttgtca tttgcacttt actttttttt cgtgtcttct tttctttatg catctgattt
     8101 tgtccatgaa aagtcagctt tgcaacaaaa tgtccttcac cctgatcttt atgaggccat
     8161 atacgtattg ttttttccaa accagctacg cttccccatt cagaacgtcc tgaactaacg
     8221 gattgagtca aaggaatctc ctcaatcgtt actggataat tttctacaag ccagctaatg
     8281 atctcttcat tttcttctgg tgcaaacgta caagtcgaat aaatcagttg tcctttattt
     8341 tttagcattt ttattgctga agacaaaatc tcttgctgac gtttttgaca gtatagcgga
     8401 gattcttctg tccactcttt tattgcattt ggatcttttc ggaacattcc ttctcctgaa
     8461 caaggagcat caaccacgat ccgatcaaaa aagcccgaaa aatgagggac aagttctgcg
     8521 ggtgcatgat ttgtcactat tgcgttcgaa actccccatc gttctatatt ttctgataaa
     8581 atcttcgcac gtttcggaaa gatctcattc gtcactaata accctttccc cttcatttga
     8641 gcagctaatt gggttgattt accaccggga gccgcacaca aatccaaaac tttttcaccg
     8701 ggttttgctg cagcagctgt tccaacgatc attgcacttg gttcttggct atattcatac
     8761 ccagcttgat ggaggaaaga ctttccattg acagtgccaa gaaatccctc attgctgtag
     8821 ggagcgggtt gtaattcttc gctatggtaa gtttggacca tgtccaatcc tgccggtttt
     8881 agtggattcc accgaaatcc ttttttcacg cttccttgtt caagcgcaga aaaaaaatcg
     8941 gatgcttctt cgcctaaaag aagacgatat tttttgataa attgctgtgg taacgttgtc
     9001 gcttcctcct tcattccaat gtttcgttag acattttaca atctttttta cgaaaaataa
     9061 aggtggctga tctacttttt catgttgctg cctaaggtga ctgttccttt aagatatttt
     9121 ccaagtattc atccgtcacc tcgtttttat cccaaacagc gttcatcatt tcaagaaaat
     9181 cttgagaaga atcatctgag cgcaaacttc ccaatgcatt aagccatacc aattcaagcg
     9241 acttttcttc tttgatcttc gtgtgacctt tgacatatga gaaaaatggc cgcttaaata
     9301 gcgaatccat gtctaaatca gattttgcaa tgatttcacg aatactttca tggatagaaa
     9361 aaaaatcttt caataagtag acaatgctta aaacatcatc ggaactgcat tcagctaagt
     9421 agttttcaaa ataaacagtg atttcttcta atatcttctc ttttaacccg ctgattgatt
     9481 tgaaatgtgt ataaatcggg tatgtagaac aaccgcagaa ctctgctaac tctcttgcag
     9541 taattgactt cattccatat tcttccacgt ataccatact tttgaacagc aaatattctt
     9601 tactataatt tgttactctc cccatcattc ttacctccgt ctcttttcat ctacccataa
     9661 ataaataaca taaataattc taaaaatatt attgataatt atcaaaacat atttagttat
     9721 atccattaaa aatcaatttt tccgccttaa aaacaagaat atcttgacaa aataccctag
     9781 gaaataaaca atagaataaa caaggtaaca aaaaaattaa ataacgttat ataaaaaaga
     9841 aggcgacact ctgtgagaat aacacagact gaaatgactt tccactcaca gtttttgtct
     9901 aactatatac aaaaacagaa taaaaaggag gtatttatgc taattgaagc aagaaaaaaa
     9961 gagttacttg ttctacaaat ggtcattcaa aaaaataatt gttctttaca agaattatcc
    10021 aatgacttac atattccaaa acggacaatc aaagaactga tccgaaaaat aaatgtatca
    10081 ctcaagcaat ttttattgat tgatgaattt atttattcta atcataaagg agagatccaa
    10141 accaaagaaa ccgatcaaac taaaaagtta gctgtttttt ctaagttaaa attatgctac
    10201 ctaaaagaat ccaatcgttt taactatttg atgttactaa tcaattatcc acaaactgct
    10261 gtaccaaaaa aatatttact tgaacaactt tatatatcgc cctcctatct cgaaaaactg
    10321 acaaaacagc tcaatcattc cttaaaaaaa ttccatttac ggatcctatc tgtaaaaaat
    10381 tgctgtactc ttgaaggaaa tgagttatct atccgtctct atctttactt ttttctttca
    10441 gatacatttg gaggtaacga ttggccattt tcctcacttg aattaaatgc actaaaaatg
    10501 aacagtacag atgagcaaat agaaaaaatt tcaaaacaag aaaaaaatat ctacttgctg
    10561 ttaactttat tcatattacg aaacaaacaa caggcatatt ttccaaaaac ttcttctgaa
    10621 atcactgaat tgatggactt attgcagcat acacatgatt attcagaata ctttacttct
    10681 ttttcagtga gtcgcccttg tgaagagaca gaaacagaga gagcctactt taattatttt
    10741 ttctattgtt tttttccaaa tcaaatctct tttcgacaaa aacaggagat tggtttttta
    10801 ttttccacaa gcaaaaacct tatttgtcga caagtcggtc aattgatgac ttacttaact
    10861 caacagtttc ctctcaacca tttagaaaat aaaataccaa tttattttta ttttcttgtg
    10921 ttggcagttg tttttttcga actgttgggt gaatccagca gtagtttttc tacactttgc
    10981 tttccagaag ccacgtccaa tcattctagt tcttttatac aggaaaagaa attaagagaa
    11041 aaaatcgata caattttttc aaaaaatggg gaaagaaacc aaatcagtca ataccttagt
    11101 caattgatcc aacacttact acaaacagaa acgaaagagc agttactcat ccatttgcag
    11161 ataaccaaac agtttacagg aaattatcat cttcaaagcc atttgcagca gttatttcgc
    11221 aaagatgctg ttaaaattac cgcatattca gaacatgctg atttgatcat tacagatcag
    11281 catgagaaaa tttcagatga aacggagact ttctatctgg atacaacgca caatacagtg
    11341 ttatttggca agttaataaa agcggttcat gaactgtata tgaaaaaagg ttatgagaac
    11401 aaaacaaaga ggatgtgaca aaattcttgt cacatcctct
//
LOCUS       DAFFFQ010000088        11329 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000088
VERSION     DAFFFQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.089"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(51..560)
                     /locus_tag="LQB69_002467"
     CDS             complement(51..560)
                     /locus_tag="LQB69_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526599.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDVSPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(550..1275)
                     /locus_tag="LQB69_002468"
     CDS             complement(550..1275)
                     /locus_tag="LQB69_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526600.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(1498..3618)
                     /locus_tag="LQB69_002469"
     CDS             complement(1498..3618)
                     /locus_tag="LQB69_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL3526601.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3848..5026
                     /locus_tag="LQB69_002470"
     CDS             3848..5026
                     /locus_tag="LQB69_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL3526602.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5042..5800
                     /gene="aroD"
                     /locus_tag="LQB69_002471"
     CDS             5042..5800
                     /gene="aroD"
                     /locus_tag="LQB69_002471"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL3526603.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5823..6308
                     /gene="ybaK"
                     /locus_tag="LQB69_002472"
     CDS             5823..6308
                     /gene="ybaK"
                     /locus_tag="LQB69_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL3526604.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6379..7632)
                     /locus_tag="LQB69_002473"
     CDS             complement(6379..7632)
                     /locus_tag="LQB69_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL3526605.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7749..9098
                     /gene="gdhA"
                     /locus_tag="LQB69_002474"
     CDS             7749..9098
                     /gene="gdhA"
                     /locus_tag="LQB69_002474"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL3526606.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9210..10556
                     /locus_tag="LQB69_002475"
     CDS             9210..10556
                     /locus_tag="LQB69_002475"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL3526607.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(10864..11250)
                     /locus_tag="LQB69_002476"
     CDS             complement(10864..11250)
                     /locus_tag="LQB69_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3526608.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3683 a         2032 c         2299 g         3315 t
ORIGIN      
        1 atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg
       61 ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag
      121 ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc
      181 aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag
      241 gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta
      301 agcaaggaag gagagacatc aagtttttct tgataggcca aacgatcttg tttgatttct
      361 ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa
      421 tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc
      481 tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga
      541 atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa
      601 agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc
      661 aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt
      721 ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg
      781 cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga
      841 ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag
      901 ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat
      961 caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa
     1021 tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg
     1081 aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc
     1141 taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct
     1201 tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata
     1261 gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag
     1321 tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag
     1381 aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta
     1441 ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca
     1501 ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc
     1561 aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt
     1621 ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg
     1681 gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac
     1741 agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt
     1801 aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata
     1861 ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa
     1921 tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt
     1981 aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg
     2041 gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga
     2101 acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg
     2161 gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa
     2221 gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc
     2281 atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata
     2341 atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg
     2401 tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt
     2461 aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt
     2521 tcgattccag aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg
     2581 acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga
     2641 gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc
     2701 tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact
     2761 attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat
     2821 caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc
     2881 gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat
     2941 atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata
     3001 aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt
     3061 cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc
     3121 taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt
     3181 ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg
     3241 gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat
     3301 cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag
     3361 caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat
     3421 ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa
     3481 gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt
     3541 caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg
     3601 tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct
     3661 gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt
     3721 caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa
     3781 tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg
     3841 tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc
     3901 actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt
     3961 agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat
     4021 cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt
     4081 tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg
     4141 tatattcaat ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta
     4201 tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc
     4261 ttttcgtgaa atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt
     4321 aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa
     4381 agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt
     4441 agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt
     4501 gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga
     4561 aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt
     4621 gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa
     4681 atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc
     4741 cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag
     4801 tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc
     4861 gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga
     4921 acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg
     4981 caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag
     5041 aatggttcgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct
     5101 tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc
     5161 cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc
     5221 agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg
     5281 taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga
     5341 acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag
     5401 tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag
     5461 tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga
     5521 acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt
     5581 gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta ttattacgat
     5641 gtccatggga aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat
     5701 gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc
     5761 attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt
     5821 ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga
     5881 ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg
     5941 ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg
     6001 tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag
     6061 caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga
     6121 ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt
     6181 tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac
     6241 agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa
     6301 aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc
     6361 ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt
     6421 agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc
     6481 cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag
     6541 agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag
     6601 cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc
     6661 tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat
     6721 accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg
     6781 aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc
     6841 cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat
     6901 accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt
     6961 taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata
     7021 cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac
     7081 tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc
     7141 agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat
     7201 tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag
     7261 ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa
     7321 catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat
     7381 atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg
     7441 tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat
     7501 cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc
     7561 agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata
     7621 cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa
     7681 aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc
     7741 gtggaacgat gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa
     7801 aggatcaagg gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt
     7861 cttttttgga cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac
     7921 cagaaagagt tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa
     7981 atagagggta ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct
     8041 tccatcctag tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga
     8101 atagtttaac cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag
     8161 gaaaatctga taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac
     8221 atatcggccc atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg
     8281 gctatttgtt cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta
     8341 aacctttgga tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt
     8401 attatgtcaa acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg
     8461 tttccggaag cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga
     8521 aagttgttac atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga
     8581 aactattgaa agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac
     8641 gtccttctgc tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata
     8701 tcgctttgcc atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag
     8761 aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt
     8821 ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag
     8881 tagcagtatc tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc
     8941 aagtggatga gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag
     9001 cagccgagta cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa
     9061 aagttgccaa agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat
     9121 attgtcagca ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct
     9181 gtagagtaaa cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag
     9241 tagcaccgtt tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc
     9301 acaacgaatt acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac
     9361 caacgaacta tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat
     9421 ctgattctga agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag
     9481 cgatcgactt cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac
     9541 cacaaatctt ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag
     9601 taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac
     9661 cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag
     9721 caaacaaacg tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag
     9781 atgcagaagg ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat
     9841 taactgctgt tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc
     9901 aaggtgccgc tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt
     9961 accaatacgc agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga
    10021 tcaactatga accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat
    10081 ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc
    10141 actctttagg acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg
    10201 ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag
    10261 gctacttgca aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc
    10321 ttgctcatac agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt
    10381 atacattggg ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga
    10441 atggaatcaa cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac
    10501 ttctaggcaa accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata
    10561 agggtttttc gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa
    10621 ctggtaccca tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga
    10681 caagacgttt gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag
    10741 tttgttttag gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt
    10801 tttctagaat cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt
    10861 agcttaagat aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac
    10921 ttttttctct atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta
    10981 agcagaacca ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc
    11041 accgttttca gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt
    11101 atctgctttg atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt
    11161 acctacataa gtagactgat agtagtccca acctttccat cctataaaag caatcaatat
    11221 gataacgatc aaccctaaaa tttttttcat gaaagatctc cttttttatt tttattatac
    11281 ccgtggtgct agttaatttt aatatcctaa agaatatctc aaagttcct
//
LOCUS       DAFFFQ010000089        11225 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000089
VERSION     DAFFFQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.090"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LQB69_002477"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LQB69_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL3526609.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LQB69_002478"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LQB69_002478"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL3526610.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LQB69_002479"
     CDS             complement(2169..2906)
                     /locus_tag="LQB69_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL3526611.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LQB69_002480"
     CDS             complement(2997..3524)
                     /locus_tag="LQB69_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526612.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LQB69_002481"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LQB69_002481"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL3526613.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LQB69_002482"
     CDS             complement(4401..4886)
                     /locus_tag="LQB69_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL3526614.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LQB69_002483"
     CDS             complement(5039..5236)
                     /locus_tag="LQB69_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526615.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LQB69_002484"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LQB69_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL3526616.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LQB69_002485"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LQB69_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL3526617.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LQB69_002486"
     CDS             9211..9423
                     /locus_tag="LQB69_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL3526618.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LQB69_002487"
     CDS             9425..11104
                     /locus_tag="LQB69_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL3526619.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3252 a         2426 c         1971 g         3576 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tctta
//
LOCUS       DAFFFQ010000090        11211 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000090
VERSION     DAFFFQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.091"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(153..1193)
                     /locus_tag="LQB69_002488"
     CDS             complement(153..1193)
                     /locus_tag="LQB69_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3526620.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1542..1868)
                     /locus_tag="LQB69_002489"
     CDS             complement(1542..1868)
                     /locus_tag="LQB69_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526621.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1855..2658)
                     /locus_tag="LQB69_002490"
     CDS             complement(1855..2658)
                     /locus_tag="LQB69_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3526622.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3011..3301)
                     /locus_tag="LQB69_002491"
     CDS             complement(3011..3301)
                     /locus_tag="LQB69_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL3526623.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3437..3616)
                     /locus_tag="LQB69_002492"
     CDS             complement(3437..3616)
                     /locus_tag="LQB69_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526624.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3613..3963)
                     /locus_tag="LQB69_002493"
     CDS             complement(3613..3963)
                     /locus_tag="LQB69_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526625.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3956..5281)
                     /locus_tag="LQB69_002494"
     CDS             complement(3956..5281)
                     /locus_tag="LQB69_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3526626.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(5450..6034)
                     /locus_tag="LQB69_002495"
     CDS             complement(5450..6034)
                     /locus_tag="LQB69_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526627.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(6245..6448)
                     /locus_tag="LQB69_002496"
     CDS             complement(6245..6448)
                     /locus_tag="LQB69_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526628.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(6458..6880)
                     /locus_tag="LQB69_002497"
     CDS             complement(6458..6880)
                     /locus_tag="LQB69_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526629.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            7194..7808
                     /locus_tag="LQB69_002498"
     CDS             7194..7808
                     /locus_tag="LQB69_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526630.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7973..8977)
                     /locus_tag="LQB69_002499"
     CDS             complement(7973..8977)
                     /locus_tag="LQB69_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526631.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
     gene            complement(9436..9693)
                     /locus_tag="LQB69_002500"
     CDS             complement(9436..9693)
                     /locus_tag="LQB69_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL3526632.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(9686..9955)
                     /locus_tag="LQB69_002501"
     CDS             complement(9686..9955)
                     /locus_tag="LQB69_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3526633.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10086..10265)
                     /locus_tag="LQB69_002502"
     CDS             complement(10086..10265)
                     /locus_tag="LQB69_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526634.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(10646..>11211)
                     /locus_tag="LQB69_002503"
     CDS             complement(10646..>11211)
                     /locus_tag="LQB69_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526635.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3424 a         2042 c         1601 g         4144 t
ORIGIN      
        1 ttaaaaccta cgtttgtgat tgttttcata aacttttaat ctaaaaactc cggaataata
       61 gctatttatt tgtaacattt gtaataaaat taaaatcata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcgacaaacc aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca
      301 gttttttgtt tttgataagc tttcagcaaa gtcatatata atcctttgtc agcatcaatt
      361 ccataagatt ccagttcttc ataaacaaca actgtttgct ctaattcttc agcttttttc
      421 tttgcattat gaatagtttg gacggttttt tgagcgtctt cgtaagtact agaaaatgtt
      481 tttatcaagt ttaatacatt ttctggtaca aatgtattta tactgtcctc tttcatgatg
      541 tcgacgaagt tatctagtaa aacttgattt tgtaactgat cagtttcagt gtctattatg
      601 tgtctattag tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg
      661 gttgagtcct tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtggta
      721 tcattgattt ctcgcggttt tgccgtattt gcgattttcg caattacggt ggtatcagta
      781 ttgtcttcct cttccatgat tttaaagacg tcttgattcg ttacttctgg cttgcctaag
      841 tacaataaat taggcatgga ttcaatctta ccgtcacgtt tatttactct tcctctcttc
      901 tgtttcagaa gacctgcttt ttctaattcc tttttgattt tagatacttt accttcgcgg
      961 cattgtaaca atttcattaa ttcttcaact gtgaatatga aaaatatatt atcatcagaa
     1021 tctacccagt tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt
     1081 tttgcatcat ttgatagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga
     1141 taatattttt cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat
     1201 cctctttctg taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata
     1261 tctatccact atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt
     1321 ctttttttat ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc
     1381 aaggtttaag ctcatagtaa ctatatcacg atattacaat aatattatat cacgatatcg
     1441 taatattaca atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa
     1501 aaaatatcac gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc
     1561 tttgctagtt tatatccttc aatcaagtat tcttcaggta tggactttaa tagcgcatct
     1621 tctataacgt ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct
     1681 gacattagaa ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct
     1741 tttatgactt tacttatatt tttcttggtt aattcgattt cttctacttc ttttgattta
     1801 aaattctttt tatttttatt tcctagtaat ctacttactg cagcttgact gcgattatct
     1861 tctgccatca aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc
     1921 ttcagctgtt gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc
     1981 aacggttatt gtcctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat
     2041 ttcggttgct gcagacgtca tttctttaga aatgttataa cgcttattat acttagtaaa
     2101 tagaactcct cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc
     2161 acttaacttt attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc
     2221 atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat
     2281 aatataatca tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc
     2341 tacttgagta aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat
     2401 tcctttatag tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat
     2461 tgtaggttgg tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg
     2521 atcaatgtcc actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt
     2581 aacgctagtc gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc
     2641 cattaaaata tcccccattc atcttatatt ataatataat tatatcataa tattgtgata
     2701 ttgcaatatc gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat
     2761 atatcggttc ggatataaag attattcata tgttattcga aattaaaata atcacttttt
     2821 attactttaa aattttttat taatgataag aaatatgttt tgcgagcagc aactagaata
     2881 atttaaacat tactaaactg tctatattgt tactaataat tcccttctta tagtgcattt
     2941 tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt
     3001 gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat
     3061 attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca
     3121 ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc
     3181 agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc
     3241 ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca
     3301 tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat
     3361 tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata
     3421 gaatccgtaa aataccttat atatataacg ggaagatata aaacctaaaa ttatgataac
     3481 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     3541 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     3601 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     3661 tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa
     3721 gatcttgcca atgatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat
     3781 agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt
     3841 catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc
     3901 agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat
     3961 catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc
     4021 ttctagcgcc ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc
     4081 gaatcttgtc aataatttta tctaaatttt cttcgtcaat ttgcttttgt ggatcactaa
     4141 ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacatgcc
     4201 ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     4261 ttgtattatc tgtgataggg atcttcatct ggtgtgaaaa ccctggtctc gtttctaaga
     4321 tcgacgtgcc aatgtgtaaa tggacacaac ctgttttaca gttatgctgc ctaatgcgta
     4381 tcgctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     4441 ctcgtggtaa cacttgtgaa tttccatatg atttttcctt tgtcggttct ggtgggattg
     4501 ctatatctgt tcgatcgatc ccattagcat ggaagtacag ctgtaatcca atcacaccta
     4561 atctattctt aatcgtataa ggattccagt tagcaaggtc tctaatgctc aaaattccca
     4621 tttggttgag cctttttttc attcgcgaac caattcccca aaagtccgtc atttcgggaa
     4681 tattccatac tttctcagga acgttttcgt acgtccattc cgcaataaat ccttcattat
     4741 gctttgcttc attgtctaag gctaatttag ccaaaagggg attatcgccg acgcctaccg
     4801 tagcaatcaa acctaactct tctttaatac gttcttggat catttgagcg agcttcttcc
     4861 ttcgctgact tcgtgtttct tcagtcgtaa aaagattcag tgattttgtc acttttaaaa
     4921 tcgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt ctgaaaatat
     4981 tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca acgtgtagtg
     5041 ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata ccataccgct
     5101 tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca gatcgactca
     5161 taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg acacttgcgt
     5221 aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa taatccatca
     5281 taaataacac tccattcttc cacattatac aaacatatgt tcgatgttgt aaagaagaaa
     5341 tagctaaata tttgagaata agttatcagt agacacttac aaaaaattct catactattg
     5401 tcacttttct agataaatgg caaaaatttt cagcattaaa tgacaaaaac taatgagaat
     5461 ttgtctttcc atttttctcc tccatttttg ccaaataacg ataaatggtg gttcttgaga
     5521 cgttccaccg ttccgcaata gtcttaatag gtgtaccgct atccactaaa gtcttcagta
     5581 attccaaatc ttgttcagac aatttttcag gacgaccacc aaaccgtcct cttgcacgag
     5641 cagctgctcg tcctgctgcg gatcgttcca ggatcaaatt acgctcaaat tctgcaaatg
     5701 ccgcaaacaa atgaaacatg agctgtcctg tagaacttga tttgtccatc gtaatatttt
     5761 cttgaatact atgaaaactc acaccacgct cattcagtcg attgacgatt gaaatcaaat
     5821 cttccatatt tctacctaaa cgatctaagc gccaaacgac aagtgtatcg ccagagcgaa
     5881 caaaatcaat ggccatttct aatcctggtc gattactttt tgccccactc atatgatcag
     5941 taaaaatttt ttcacaacca aatttattta ggctgtcttc ttgtaaatcc aaattttgta
     6001 aaccagtaga tacccgggcg tatcctattt tcaaaaaaat cactccttca ttaattttat
     6061 tgtaacataa ctttatccat atagtttaac tgaacataga tattgttatg ggtttatgaa
     6121 catgaaatga attgtttttt tgaaaaataa agcaaaaaaa gaaagtgtcc agaaatgggt
     6181 gattttatga acactttcaa ttagaaaaaa agaatgtcga ctagacactc tttttttaaa
     6241 atcattattt ttctttttga tatgtttttt ggtctctttt ttttcttttt tcttcagaat
     6301 cttctagatt agaaattgat ttttgaattt tttgtgtctc ctgtaaagtt cgctgaactt
     6361 gtaaagctga ttcttctttc ggtaaattaa ttttacttag aatatcgcta tcttgataaa
     6421 aatttggtgt gttcacaaat tctggcataa aaaatcccta ctttctagtt aaggttcctg
     6481 aataagtcgc aatccaattg tttccagctg atttataact ttgtaaatac aattttccat
     6541 agtaccaata accatttctt ttttctttcc aattccgtga ttgaggaggt ctagaaggcc
     6601 aaccaacttt atcaaaaatc atcgttcctg tcactcgtat cgttttagaa gctgctcgag
     6661 caagtaatgg agaaactatt aaataatttt cagtaacagt taagtcgtta gttgggttac
     6721 caatggctat tccgttccta tctaaaggag ttaattcttg gttattatct actacagtag
     6781 ttggttcatt tgtactatca gcaaatgtag gaattgtagt tccagttaca ctaattccaa
     6841 tagttaaaat acctaaagat aaaataaact tatttttcat tgtctatcac ctcgaaatca
     6901 aaaataagaa aacgttttct taataaatct taacagataa atcaagttat atactagaaa
     6961 attcataaaa tatattttta atgtcataat cgtttaaatt ttcttgattg acatttattt
     7021 acaaaaaata ttgccattta aaaagaaaag tgacacatac tatttcctcc atcattagtt
     7081 tttgtctata taaggtttac cctaaataga cactggtata acattgaaaa atatggcttc
     7141 ctcgtgtatg attagggtgt attttaaata gttgcagaaa gtaggtaaaa gttttgagag
     7201 ttgcatacat acgtgtctca tcgatcgatc aaaatgaaca acgccagatt gaagaaatga
     7261 aaaaattcgg tgcagagaga attttcatcg agaaacaatc tggcgcaacg attactcatc
     7321 gacctgtttt ccaagaagca ttagattacg tccgagatca agatattttt attgtggaag
     7381 ctattgatcg acttggaaga aattatgacg agatcatcgc ttcagtcaat tacttgaaga
     7441 agaaaaatgt gaagctgatc attactagtt taccgatcat ggctgaagca attggcaatc
     7501 ctctcctgga taaattcatt aaggatctga tcatccaaat tttggccatg atcgctgaac
     7561 aagaacggac tgaatcgaaa cgtcgccagg cacaaggaat taagatcgca aaagcaaacg
     7621 gcgtctacaa agggcggccc aaattataca gtgcagacgc aaaagatccc caacgtcgcc
     7681 ttgtttacaa aagcatcgtt gaagatctta aaaatggtgt tgcgattgct aagattgcta
     7741 aagactacaa tgttacgaga caaaccgtgt atagaataaa aaaagacagc atggtcaatg
     7801 acaaatagtt gatcatgctg cttttattat cttctcatct ttaatttcct acatataatt
     7861 actatctata aaattaagtc ttactcagaa cagataaagc ataaaattcc aacaaaccat
     7921 tttttactta actaaataaa gagtctgtta atataatctt cacttattta cgttaatccc
     7981 ctactgtatt tgttggttcc ttatctatat tttctacaaa atccttatta tcttgtgatt
     8041 tagcttcgta taattctttg tatgtcgaca cattggttat actaattcct atagctagtt
     8101 cttgagactc tacttttctc tctagctcct ttgacaaagt gatttgagat ttatttggtt
     8161 ccgttggagt caattctata gattcttcat aaaaatctct atacataaag ctatcattaa
     8221 ttttataaaa aaaattaaat gtttttagca ctgccatata acattcaaaa cattcttcta
     8281 tagttggttt acttccttga ggagaatgaa ccaatttatt acgtttttct atgaaatttt
     8341 taatttcctt tcttatttct ttcggaaact caatattaaa gtacccttta aacatgctcc
     8401 ctaataattt gtctacaggg ggagattgta aattctcaaa tattagagtt aaatcaggtt
     8461 tctcttgttg ataaaattcc tttaccccta tttcacaggc tgtaattatc gaaataaacc
     8521 tatctctagt atttgatata tttttacttc ttttaaggag tataagagaa aaggggatac
     8581 tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc agaagttata tctctaatta
     8641 tatagttaac ggaattagga agtgtcttta actctaatga aatttctatc caattactat
     8701 cttctaatct gtatatgctc gaagtataat ttgattcgtc agcaagcatc gctacatccc
     8761 aaacactgat cataaaatct tttaaacgtt caatcatttc agcaatattg ctaaaatcag
     8821 ctaatttttc tttagaataa aaaaaaacat ttatatcaga atcaataaat tctatttttt
     8881 ttattactat atcgtcatca ttatcttgac catttatgtt tattgttccc tgagtgggga
     8941 gtacttcttt caaattcaag ttaaatttat attccattac ttctcctcca atatataaaa
     9001 attacttaac tgtatccaaa tttaatatgt aaataaatca ttaattttta gctaaattct
     9061 atgagaagtt tatttttgtt gtttttgcta attaactaaa actagtacaa aaaatttaaa
     9121 atagattatt aaacctattg cttattctat tcagtcatat atataagcgt catcctttag
     9181 taactaacat cccacactac aatagtatta taacatatct attcaacttc aaattgttga
     9241 aatttaagcc atttatattt acttttcctc aactttagaa atgattttac taaccttata
     9301 tccgtaatac gcgtaacaaa cacattcatt aataacaatc aacactaagc tatattagct
     9361 ttttaaatga ctagggtaaa taaaataagg atcaaaaact tttaagtttc tgaccctttc
     9421 cttacttccg attggttaat agtgatcttt tgcagaataa ataaatatcg tttcattttc
     9481 aactctatat atcagtctat gttcatctgt aattcttctg gaccattttc cagataaatc
     9541 atgctttaat ggctcaggtt ttcctaatcc agcaaagggg gaacgatcga tatcttttat
     9601 taacttgtta atctttttta tattgctttt gtttccttgc tcatgccaat aaagataatc
     9661 atcccaagca tcatcagacc aagccttaat catcagattc aacctcgatt aagtcatgtg
     9721 ttttaaatgc acctttggag aattgttcat ctcctcgacg aattttttcc atgacgtaat
     9781 tattagaaag tgttctcaac gtttcttgca tagaatcata atctcttttt gataatacaa
     9841 caactgtatc ttctacatct ttacttgtta caataagtgt ttcagcatcc tcattaactt
     9901 gtttcatata actacgtaaa ttttggcgga aatttgaata agctactgct tccattcctt
     9961 tcacccctta tttatttcta taaaacaatt gtacattata ttgtacaatt aagcaaatga
    10021 ttaatattct aacttcttat aattaaaagt gctatcactt ttagttttta ggataaaaag
    10081 tgacgtcact ttttgagtgc tggtacctca aatcctgctt tttcttgggc aatcaataaa
    10141 tctaacgcaa tacacataat cctggattca cttttgccgt acttctgctt tagttgctgc
    10201 aattcttttt gtctcttttc atccaacgta acgggtaacc gtttatttcc tattgcaatt
    10261 ggcattagta ctctctcctt tcatcgaaat aaccctatga aaaatatggc tgcaatcatc
    10321 ttgaatagta gactcacaag ggctaataca aagcgccaaa agatcaaaca aaataccagt
    10381 attgccaaaa atactacgaa gtttaaatgc acagctgcag catggcgcat aagtatttcg
    10441 ccaacaaata ggactaacaa tagaggaatg attttattca tatggatctc cttctttctc
    10501 ttaggttatc gggtgttgcc cgcttatttc gcaccaccta agcggcgggg gcggttggag
    10561 cgattggtgc gaaataagaa ccattccctt agttactagg ttttttgtcc ttatttcaca
    10621 cattctcact tttcggtata aatgattatt gccttttttt cctttcaaat cgttgatatg
    10681 gcattcgttt acggtcaagt tcattttgga tttcttctgt atcgtcgtaa gtataggtgt
    10741 ctaaaaaatc agttaaagtc gtgtaccagt cgtcgcctgg gttatctact tcttctacat
    10801 attccctcaa aaatgcttcg ggtacagcga gttcataatc cattcccatt aacacgatag
    10861 attccgttcc acacgtcaaa aatctagtaa atacaaggta ataatcgtct atcgtcgcat
    10921 ctttgctgtt tagctcttcc atttcttctt ccattaattc catgctctca aaaattgaat
    10981 aatattctga ttctgagtaa tagtgtttcc tgcaaatcgt gtagctgaca aagttttcga
    11041 ccagctcact cctttctagg taaacattca tgatgtattt ttctgtttct agtcgtagaa
    11101 gctccgttcc tttttttacc gtttcaggac gacgtttata cacgtctaaa cgattatggc
    11161 tgactgtgac cttcgtttga ctcgtttcac tttccaaata caattgttct t
//
LOCUS       DAFFFQ010000091        10105 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000091
VERSION     DAFFFQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.092"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..432
                     /locus_tag="LQB69_002504"
     CDS             <1..432
                     /locus_tag="LQB69_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526636.1"
                     /translation="IDENGQFFVWMRHEHCWCNPFVLKKENEIRTKIANGEWDYLFRW
                     EDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQNIDIDAIECLKEVDQKYEEEPY
                     YLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDRKSSI"
     gene            607..942
                     /locus_tag="LQB69_002505"
     CDS             607..942
                     /locus_tag="LQB69_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526637.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(1199..2782)
                     /locus_tag="LQB69_002506"
     CDS             complement(1199..2782)
                     /locus_tag="LQB69_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526638.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2904..3488
                     /locus_tag="LQB69_002507"
     CDS             2904..3488
                     /locus_tag="LQB69_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526639.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            3499..3744
                     /locus_tag="LQB69_002508"
     CDS             3499..3744
                     /locus_tag="LQB69_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526640.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3750..4472
                     /locus_tag="LQB69_002509"
     CDS             3750..4472
                     /locus_tag="LQB69_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526641.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4603..5346
                     /locus_tag="LQB69_002510"
     CDS             4603..5346
                     /locus_tag="LQB69_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526642.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            5352..5717
                     /locus_tag="LQB69_002511"
     CDS             5352..5717
                     /locus_tag="LQB69_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526643.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5733..6263
                     /locus_tag="LQB69_002512"
     CDS             5733..6263
                     /locus_tag="LQB69_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526644.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            6425..6655
                     /locus_tag="LQB69_002513"
     CDS             6425..6655
                     /locus_tag="LQB69_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526645.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(6859..7338)
                     /locus_tag="LQB69_002514"
     CDS             complement(6859..7338)
                     /locus_tag="LQB69_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526646.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7458..8795
                     /locus_tag="LQB69_002515"
     CDS             7458..8795
                     /locus_tag="LQB69_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL3526647.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8818..9345
                     /locus_tag="LQB69_002516"
     CDS             8818..9345
                     /locus_tag="LQB69_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526648.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            9332..9895
                     /locus_tag="LQB69_002517"
     CDS             9332..9895
                     /locus_tag="LQB69_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526649.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
BASE COUNT         3674 a         1331 c         1904 g         3196 t
ORIGIN      
        1 atagatgaga atggacaatt ttttgtttgg atgcgacatg agcattgttg gtgtaatcct
       61 tttgtgttaa aaaaagaaaa tgaaatcaga actaaaattg caaatggtga gtgggattac
      121 ctcttcagat gggaagatga atcttttata gcaaatgata tggatcatgc atttgtctca
      181 tttctggata atacatgggg tacttggtac gattgttacc tagaaaaatt gggctttcaa
      241 aatattgata ttgatgctat tgaatgtctt aaagaagtcg atcaaaagta tgaagaagaa
      301 ccctattatc tcttgcaaca aaaattagcc aataattcag tgtttcaggt agaaatttgg
      361 gaaaatgaag atggaactta tgaggggcta gcgcaagaaa tacccttctc agataggaag
      421 agttcgattt gaaaagagta tatcagcaat ttttatgaag tgaaagcaag cttacaatat
      481 agtaatggaa aattgtggtt agctggaagg tatattccag ctaaccaaat cttacgtttt
      541 ctgggagagt ggcgttttaa aacaatttcc taggaaaaat agttaacata aaaggaatga
      601 attgttatga aacaatattt aaatgaatgg aaagcgatag aggggtcatt agtaacgcaa
      661 agaataaagg aattacctga ttgcttagag aaggatcatt tgtttcaaat acgagaaatg
      721 ttgaaaaaag aacagtttga tccagaccaa tttttagtag tcgagtatcc aactattgga
      781 gtgtactgtt gtaaccatat caatgatgga aaatatttta ttattcagga gtatggagga
      841 cagttaactc ctttctatac tacatgggaa atgagtgaag aaggaatcaa taattttcca
      901 tgtgaaagca tcgaagattc aatttcacac accgaatgtt aattgattat aactataaaa
      961 aaggattgaa cattatgttc aatccttttt tcaatgaaat gttttccgcc atttcattgt
     1021 ttggtgcgat ttctatttta ttatatcaaa ataaagagag attctaaaga aagaagaggg
     1081 aaatatataa atcaagcgac agtatattta ttattaaatg ctattgtgag aatataacta
     1141 attagtttaa tttgaaagta aatatacgct ataaaagtgc atcttgtgtt ataaaaaatt
     1201 attttatttt gtgaacataa tttaacaatt ctttctcgga attgatttct tttcttaaat
     1261 agtcttcaga cttattaata atattatcta ttatgtctag atacatatca cttttcgatt
     1321 cagctaattc atacagtttt tttaattctt tatcctttaa ttgaaaaccc tgatccatag
     1381 tcactgcatt tggatataga agcccactat aatgtttacg taaattcact tggaatttct
     1441 gattataaaa gtctaacaga ttagaaataa aaggcggaaa attactgttt ttgtaaaatt
     1501 gattatttcc atttaggttg tatagtaatt tgttaaattg ctcctgattt aatttattgt
     1561 aaatccatgg taatacttcc atatccttat ctgctaacct gaatgaacta gcatcggtaa
     1621 gatgttcgat cataaaatct atgaacatct ctttttcacc aaaatataaa tattgattat
     1681 atattgtttc taatgttgaa gatggtagcg aagaaactct cattttttgc tggagtatag
     1741 gatcaactag actatttaag tgattttgta attcttcatt gttgtttcct agaactgcaa
     1801 gggaacgtaa acggtagtaa tattctattt ctgttagtac ttgttgtttc ggtttaaaat
     1861 catttattga tatttgtaaa ttttgaattg tctcgtctga aaaatcatgt tgtaattctg
     1921 gatgggtagc tataaaagta agtaaaaaga acccttttgt atacgtgatc tgttgatttt
     1981 caccaagtga tttattaatt tcctctcttg attcagctgt gaacctatac tttgcaattt
     2041 ctttttttag aatatttaaa tagaattctt tattatcaaa aattagggca ttaagtagtc
     2101 tgaaagtact ttccctattt ctatctgctt cctcatttcc ataagataat tgaaaaacaa
     2161 acttaaaggc tgtcttacat aggttttctt tggctttttc gcttagttta tttagatagc
     2221 tattttttag tgcagagtga aattcttcag gtgtaacaga catagcatgt atgcgacttg
     2281 catattgtat aatttcgatc gttacttttt ttcctaatat tggatttttt aggaatataa
     2341 tttcatagga cttccgtata acatcctgac aagtttcttt tgttatatct cttaattccc
     2401 aagtattacc gttgtaagtg actacaggat gagctgaata atttctttga tccttaatat
     2461 attttaaatc ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta
     2521 gtagttccat ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt
     2581 ctaaaatttt cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa
     2641 ttaaatctaa aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat
     2701 aaaaactact tctaacttct tctaataatg gaataattct aggattttta acttttttta
     2761 tttcatcatc taatataaac atataaatct ccttttttta tttgtatgac aagtttacca
     2821 taaaaaaaaa ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat
     2881 atgtagagtg agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc
     2941 ctgataatcc tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat
     3001 tttatctaat tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt
     3061 tcgatataga aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag
     3121 attgggaaga aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac
     3181 aagatgctga tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat
     3241 tttatcttat gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa
     3301 aaacaatcaa ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat
     3361 ataaagatgg gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg
     3421 aacttaggga atatgtgcag aacccatata attttcaata tttagcagga agaataaatt
     3481 tagtttagga gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt
     3541 gaaggtgtta ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc
     3601 attcaaaaaa atccacaata cctattattt agtctttatg cttggattgt acaaatggtt
     3661 tgggtttcca tattgatgct gttgttttat tggatagtac gggttattct tttattgtta
     3721 aacaaaagaa aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt
     3781 tatcgacagc agcattaata acccttacag catgtggtac aactaatagt gctaataata
     3841 acaaagacac aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa
     3901 caacaatgtc ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta
     3961 ataaaggtca aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg
     4021 aaaattatgg gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg
     4081 attgtccaga ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta
     4141 gttatgctcc aacattaatg catgatatta ccataaataa accagcttac gagtattacg
     4201 cagttgctgt agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg
     4261 ctgatgagaa caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta
     4321 acggcacact atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg
     4381 aaagtacaac taaatattat atggatttgg gagcatataa attatatcaa caatcagagc
     4441 ctgttatgga cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt
     4501 aaaattaagt gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga
     4561 aacacacaag ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca
     4621 aatgaagctc atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa
     4681 ggaatttata aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta
     4741 aaggcaacaa gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca
     4801 agtgaagaaa tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa
     4861 aaacatctac tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa
     4921 aaattaagaa ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc
     4981 ttaaatgact acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac
     5041 acacaataca aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg
     5101 tatgagatga aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg
     5161 aatcaagcga ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg
     5221 atcgtaaatg aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc
     5281 ttgaagtcat caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg
     5341 agatgagtta gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa
     5401 aaatacagat gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg
     5461 atcatattgc taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga
     5521 tacatcacaa tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat
     5581 atgatctatt agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg
     5641 cagagttaaa aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga
     5701 taatgcaacg aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac
     5761 aattaataga agaagattta gagaccacag ttcagagatt agcggaacga atccattttg
     5821 caaattatca aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa
     5881 tgaaattttt ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga
     5941 agaccatgat ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag
     6001 aggcaaaaga aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga
     6061 aatgcgttga tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa
     6121 tagattttat tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag
     6181 atcatgaaca gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata
     6241 acaaggtgtt tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac
     6301 aaagaatgta tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta
     6361 tatttaaaac agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa
     6421 ctgcatgtgg aataatagat taaaaacagg attgttatta atagttattt cctgcgctat
     6481 gatgattggt atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaacgt
     6541 aatagaaaaa tgctattacg gtcaacatta ttggagtgag gaagtcagag aaaatataga
     6601 tcgtgaatat gttcaaagaa ttgttagatt accagtaagt gaactaaaaa aatgatgaga
     6661 ttatcagcat atcttttaat gtttttgaat gtggtcacgt taatctttgt agtaatcggt
     6721 gtgactttcg atttaatgga atagtgggaa atctataatg gaaaaaagag acacgaatga
     6781 acatattttt tgaatcaata ttgttgatgg tttttgcgat cagcgcttac cagaaatcta
     6841 aaaaagagta atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg
     6901 agcttgtatt ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc
     6961 atcaaagtag gttttcagta gcgatttaac tgatcgctca tcttgtttga tttggataat
     7021 ttcagcatcc aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt
     7081 atcttccaac tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat
     7141 tccaacgtga gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat
     7201 tggaatatca agtagtaacg aggatgtttc ttttactgac tttttcttta cagcattttt
     7261 tgtttttcca tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc
     7321 acttgtttta tcagtcattt attagctttt cctttctatt aatttatgtt attatattat
     7381 cataataaca taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa
     7441 agattgccac attgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca
     7501 caataaatcc gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact
     7561 gtactactga aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg
     7621 ctgtaacagg tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat
     7681 atggcttatc aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta
     7741 ttgatcctcg agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca
     7801 aacaatttac tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata
     7861 cagcattagt tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc
     7921 aattggactt tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt
     7981 taaatgcttc tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt
     8041 cagctgattc cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga
     8101 agtacatgaa aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat
     8161 ccggaggttc aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag
     8221 tagaagatgc ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag
     8281 aaggaaaaat taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt
     8341 tgaaaaaata tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt
     8401 caccttttaa cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc
     8461 aattatacga tgggaatcaa ccgactactg gaaatggtgg ttatgtatgg gaaagctaca
     8521 aatcggcagg agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcag
     8581 atggatggtt atgggctgtt ggcgatgcag ggcatactgg tgtagttata gcggtgtttg
     8641 aagatgggtc atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg
     8701 gagtttcggt aactttgatt gatggagtgg acggatcaga taatgtgcat ttctttagcg
     8761 gtgtaggaaa agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg
     8821 aatagtaaag tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg
     8881 cttgttggaa tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt
     8941 gtgcatattg aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga
     9001 ttacggaaag atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaataag
     9061 tttgcagatc aatcaggtga agaatttttg agaaaactat ttattgtaac aggtaaacca
     9121 ccaattactg ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt
     9181 aagctaacaa actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat
     9241 tttgaagttc aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat
     9301 aatgataaga aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa
     9361 aaaggcgatc ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa
     9421 gaaaaatgct tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga
     9481 agtagtcaag aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg
     9541 gaatgctgaa atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc
     9601 atgggaaaaa tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa
     9661 gtctagtcct gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag
     9721 ctatactgct gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca
     9781 gacaagaaat actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg
     9841 agaaaatcaa ttgttttact tggaggattt agatagctat gtaagagcgg agtaaaataa
     9901 aaaaagagct aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc
     9961 gaaaaaggat tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct
    10021 cagaattttt attaatcaat tcatttagat tataataaat tgagctgaac attacaatac
    10081 gtatatgttg taaaacgtaa aaaaa
//
LOCUS       DAFFFQ010000092        10101 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000092
VERSION     DAFFFQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.093"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(390..1337)
                     /locus_tag="LQB69_002518"
     CDS             complement(390..1337)
                     /locus_tag="LQB69_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBL3526650.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="LQB69_002519"
     CDS             1525..2712
                     /locus_tag="LQB69_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBL3526651.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="LQB69_002520"
     CDS             2693..3997
                     /locus_tag="LQB69_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL3526652.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="LQB69_002521"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="LQB69_002521"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBL3526653.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="LQB69_002522"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="LQB69_002522"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBL3526654.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="LQB69_002523"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="LQB69_002523"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBL3526655.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="LQB69_002524"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="LQB69_002524"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBL3526656.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="LQB69_002525"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="LQB69_002525"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBL3526657.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="LQB69_002526"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="LQB69_002526"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBL3526658.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="LQB69_002527"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="LQB69_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         3530 a         1573 c         2002 g         2996 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta t
//
LOCUS       DAFFFQ010000093         9328 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000093
VERSION     DAFFFQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.094"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            317..1102
                     /locus_tag="LQB69_002528"
     CDS             317..1102
                     /locus_tag="LQB69_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526659.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1115..1966
                     /locus_tag="LQB69_002529"
     CDS             1115..1966
                     /locus_tag="LQB69_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL3526660.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2090..2542)
                     /locus_tag="LQB69_002530"
     CDS             complement(2090..2542)
                     /locus_tag="LQB69_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526661.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLMQTDISLVS"
     gene            complement(3007..3375)
                     /locus_tag="LQB69_002531"
     CDS             complement(3007..3375)
                     /locus_tag="LQB69_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL3526662.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3525..4007
                     /locus_tag="LQB69_002532"
     CDS             3525..4007
                     /locus_tag="LQB69_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL3526663.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSEHMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLLNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4044..5054
                     /locus_tag="LQB69_002533"
     CDS             4044..5054
                     /locus_tag="LQB69_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL3526664.1"
                     /translation="MISYPLLERNTTIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGINLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5169..5882)
                     /locus_tag="LQB69_002534"
     CDS             complement(5169..5882)
                     /locus_tag="LQB69_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526665.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSSDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPTHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            complement(6072..6662)
                     /locus_tag="LQB69_002535"
     CDS             complement(6072..6662)
                     /locus_tag="LQB69_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL3526666.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7185..7856
                     /locus_tag="LQB69_002536"
     CDS             7185..7856
                     /locus_tag="LQB69_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL3526667.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            7853..8014
                     /locus_tag="LQB69_002537"
     CDS             7853..8014
                     /locus_tag="LQB69_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526668.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMKYVADEANRK
                     QRKLVGLDK"
     gene            8498..9268
                     /locus_tag="LQB69_002538"
     CDS             8498..9268
                     /locus_tag="LQB69_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBL3526669.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT         3209 a         1445 c         1763 g         2911 t
ORIGIN      
        1 ggctgtgaca aaagtcttgt cacagccttt ttttccaaat aaatggtttt tcagaagtag
       61 cttcttcgga aataagccga aatttcacaa aaatttgaga agtaattttt gtaaattact
      121 tcttatttct tgaagctggc cacttctgtc acaacctctt tctttataga aataaaagga
      181 cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac tatatatctt
      241 ggtgtacact cactttaaga cggaaataaa agttatccgt aataacaggt ttaggtatga
      301 aaaaggagtg ggtactatgt ataaggaatt agaaaacaaa gtcgcagtag taactggtgg
      361 ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa tgaatgtagt
      421 tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc
      481 aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa tacagcgttt
      541 acttgatacg gctttagaaa attttgggac actggacgtt tggattaata atgctgggat
      601 ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag tattaaaagt
      661 caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta cagagaatga
      721 taaaaaagga aatatcatca atatgtcttc tgttcatgaa caaatcccat ggccaacgtt
      781 tgctcattac gctgcttcaa aaggcggagt aaaactattc accgaaacgg tagcaatgga
      841 gtacgcacca aagggaatca gaatcaatag tataggacct ggtgctatcc gtacacctat
      901 taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta tgatcccact
      961 aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg cttcagacga
     1021 atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt tatatccttc
     1081 tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg cattgatccc
     1141 ggcagttggg tgggggatcc agccactgat tttgaaaaag attggtggca gaccaacaaa
     1201 tgagatatta ggaactggta ttggagccgt gctcgttggc ttacttgttc aattattcgc
     1261 ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg gtgcattctg
     1321 ggtcatcgga caaacaggac agtatacatc tttcaacttg attggtgttt ctaaaacgat
     1381 gccaatttcg actgcattac agttagtcgg aacctcattg attggtgtct tcgcttttgg
     1441 agaatggtct ggaagtatgg gaaaaatcat tggtgcagca gcaatcattc ttcttgtatt
     1501 tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg
     1561 aatcagtcta ttagcatcaa caagtattgg ttattgggtt tacagtgcat tgcccaaact
     1621 agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct ttttaggggc
     1681 agtgatttat gtgattttcc gaaaaccaac cgcattttct gaagacaaga gttggaaagc
     1741 aacaatagtt ggagccatct tcagtgtgtc agcattggct tatatttttt cagcaaatga
     1801 aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct ctacattagg
     1861 cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa cgattatcgg
     1921 attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat aaagatggta
     1981 aaatctcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt ataaaaaaat
     2041 agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt taagatacaa
     2101 gtgaaatatc tgtctgcatc aataattttt cattgatcca ttgactaaag tttacgacat
     2161 aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga tcgtattgta
     2221 aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat tgttctcgga
     2281 attgattgat tgctgtaaaa tcacgatact gaaggttgaa acgcatcgtg attggtgcat
     2341 ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg tataggaact
     2401 caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct tttagttttc
     2461 cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg gctaattcat
     2521 ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta ctgacttagt
     2581 tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa
     2641 tcaagtcaga aaattctaac ttttctttct ctaaagaaat tcgttctctc ctaaagtgat
     2701 attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac tgatattttc
     2761 tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat aattttacta
     2821 agtggaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa gactccttat
     2881 aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa aggataggat
     2941 tgaacaaaac ctgtataagc tgatgaatag tttcctcgcc agtaaaaggt ttattcgtat
     3001 aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa atgaaatcaa
     3061 ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga cgttatctgt
     3121 atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg ctttttccgg
     3181 tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac catggtcagt
     3241 aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt ctgttatctt
     3301 agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatctgat tgatgatcgg
     3361 ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt taccagaaaa
     3421 acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg ctatatttat
     3481 atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt gacttggcaa
     3541 agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc atttatacaa
     3601 atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag accaaaagct
     3661 tattcttaaa agataaaaaa gataataact acgtatatct gacttttact acgaaacaaa
     3721 cggatttcaa agcattgggg aagatcgtcg gtaaaaggct ttctattgtt tcatcagaac
     3781 acatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt tatgaaaaac
     3841 agattccgat tattattgat gaagagctct tattaaatga aaaacttgtt tttgcaccag
     3901 gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc atccagctac
     3961 ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca agtaaaatag
     4021 tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga aatacaacga
     4081 ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta ctcgaatcag
     4141 ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg ctttggacgc
     4201 aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat caaatgatgc
     4261 aagatgaaaa tatccaaatg atctttccgc cttggggagg agaattattg attgagattc
     4321 tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat tcagatatta
     4381 gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat ggaatcaacc
     4441 ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa agtgtactga
     4501 aaacaagaga aggagaatcg attctacagc aaccttctga aaagtatcaa aaagagtggc
     4561 cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat tggcaatcag
     4621 tcagcggaga tgcagtaagg atgaagggac gattattagg tggctgtatt gatacgatca
     4681 gtcatttaat cggtacccct tatggagatg tccaatcttt taaaagaaag tataatgatg
     4741 gaaagccgat cttgtggttt ctagaaaatt gtgagctatc gtcagttgat ttaagaagat
     4801 cacttgttca gatgaaactg gcaggatggt ttgatggcag ctcggggatc atgttcggta
     4861 gaagtgcagc agaggatttg ttagattata aaatgaagga tgtatacgaa gatctgtcaa
     4921 aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg ccacaactga
     4981 cattgatcaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg actgttttgc
     5041 aaacgtttat ttaaaaaatt ttttcctgcc ttggagttta tcaaagacca acaaatctaa
     5101 atagaaaaga aaatgaaaga agctgacagt attttgccag cttctttttg tatgatctat
     5161 tagaaatttt aattatccat aactatcaca ttgatcagct gtttcacatc atttggatta
     5221 cctgtaggaa tagctactac agatgcatta tcaataattt cttgagttgt ttctctaatg
     5281 aacgctggat aagatgtttg atctgcaggc atttgccaat gtgtaggaac tggataacta
     5341 gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact gtatttccca
     5401 gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg ggcgataggt
     5461 gttcctgcag gataatgaat aacatgtagt tcgaaatcac tcgatgtatc tctaaaagta
     5521 ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga atatcctgct
     5581 aacggatcta ttgactgaga agactgtgag gaagttgtgt ctattgttga agaagaaata
     5641 tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac tttactgctt
     5701 gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt ttcagctgaa
     5761 ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt ttctttacta
     5821 ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc taactttttc
     5881 atataaaata cctcattatt tttttaatac cagtatagcg taatcctgaa tttttgaatc
     5941 cgtaaaacct tgtctatgaa tataaataga gtatatcttc tttgataaat gtcagaaaaa
     6001 gtgtgacaaa taaccatcag taaaattact tgtcatacta ttgagaagga gtatttaatt
     6061 gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata gaatccaagt
     6121 ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac tgcttgaaga
     6181 atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt acgatctttc
     6241 acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc taaccaagga
     6301 aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc taaataatca
     6361 actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc gattatttga
     6421 tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa aaggtcaact
     6481 ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc aatggaagtg
     6541 acctctgatt ggatagaagc agttttgttt tgccatccct ctttttggtt tagtgtgcta
     6601 gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat cgcagttttc
     6661 attttcaagc tctcctttac tatctatgac aaggacaaca gttttaacat acagacattc
     6721 attaaatttg gatagcgaca gcgaaaacaa caattcttaa gttttagaca gagtatacag
     6781 acagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt tttgtttacc
     6841 agcattataa cattttgcat acatggatga caaaagaacc aattcccact tgcttgagta
     6901 caatcttaaa agaaactgga aggcaaaaag taattataga aacgaaaaat aaatcatatc
     6961 gggcactcag acatgaaaac aaccctagaa atatataact agtccaatga aaaaacagcg
     7021 aatgccgtag aaaacaagga ttctgcgtac attcatatct cattttgcga gcatatctgc
     7081 tattactgtg actaaatgtt aatatggtta aaatgaccgt tatatttttt atataaatat
     7141 cgtataatta gtatgaatat agaagaagga aagaaggtgc aatcttggaa cctaaagaca
     7201 agtatttaga ttacgctagg gtcaataaag ataaatttat tgaaacaatc atcaagggaa
     7261 aaatccctga tgatgaaaaa gatgcggtat tcatggctgg aagcccagga gcaggtaaaa
     7321 cagaggttgc actaggttta gctgaaaatt atgacaacca tgttgtgatt gatgcggact
     7381 attttagagc acaatttcca gaatacaatg gaaagaactc aagtgttttt cagaaagctt
     7441 cttcttggtt agttgaacaa tcattaaaat atgtactgga acatggctat tctttcatac
     7501 tagaggatac ttttgctatc cttagtgcag agaaaaatat tattcgagcc cttaaaaata
     7561 actttcgcgt tacgattttc tatgtttacc aagatcccaa agttgcctgg gatttcactc
     7621 gaaaacgtga actagctgaa ggacgtcatg tgccaaaaga aatgtttatc aatgcgtttt
     7681 ttaagtccag agagaatatt gaaaaagtca aaaatagaca tcctgaagta gtccttcaca
     7741 taatgattaa ggattatcaa aacgacattt ctgaagtgca ttatgcagca gataatattc
     7801 aattagtact acccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     7861 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     7921 aaacgcctca gcgaaagaat ttaaaacagt aatgaagtac gtagcagatg aagcaaatcg
     7981 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     8041 ttgagttttt tgttttaatg aattaagact attaaataga taattattgt aacttaaaaa
     8101 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     8161 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     8221 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     8281 tgtttttaca taagaagact tggggtattt tttagtttga ttagctctga tatacctata
     8341 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatgtt
     8401 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     8461 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     8521 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctgatt taattagaaa
     8581 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     8641 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     8701 ttcagagaag tttaaatgtt tcaaacaggg tggaatgaga agatctcatc aaactaatac
     8761 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     8821 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     8881 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     8941 accaaaagaa cacacgtatc tgggagaagt atatttagca ggacaaatat atactgttaa
     9001 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     9061 tcaagagcaa ttgattgaag ataaagatat atataaccag gaagaaaatc aaactagaca
     9121 tattcgtaat ttatctgatg ctaaattaga ggaagaagca agaaaagtta gtaattataa
     9181 taaaaaaatg gctgcaaaat atgttaacga agaggagttt tgcttctcaa aaacaaaaat
     9241 atataaacgt acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
     9301 ttcaaaacgt cgttgtgctc gacttaat
//
LOCUS       DAFFFQ010000094         9316 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000094
VERSION     DAFFFQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.095"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..2122
                     /locus_tag="LQB69_002539"
     CDS             <1..2122
                     /locus_tag="LQB69_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526670.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="LQB69_002540"
     CDS             2119..2739
                     /locus_tag="LQB69_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526671.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            2853..2990
                     /locus_tag="LQB69_002541"
     CDS             2853..2990
                     /locus_tag="LQB69_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526672.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            3432..3812
                     /locus_tag="LQB69_002542"
     CDS             3432..3812
                     /locus_tag="LQB69_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL3526673.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="LQB69_002543"
     CDS             3794..4708
                     /locus_tag="LQB69_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL3526674.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="LQB69_002544"
                     /pseudo
     CDS             4948..5637
                     /locus_tag="LQB69_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5757..5843)
                     /locus_tag="LQB69_002545"
     CDS             complement(5757..5843)
                     /locus_tag="LQB69_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL3526675.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6488..7225
                     /locus_tag="LQB69_002546"
     CDS             6488..7225
                     /locus_tag="LQB69_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3526676.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7253..7792
                     /locus_tag="LQB69_002547"
     CDS             7253..7792
                     /locus_tag="LQB69_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL3526677.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            7808..7978
                     /locus_tag="LQB69_002548"
     CDS             7808..7978
                     /locus_tag="LQB69_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526678.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(8290..8751)
                     /locus_tag="LQB69_002549"
     CDS             complement(8290..8751)
                     /locus_tag="LQB69_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526679.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
     gene            8884..9258
                     /locus_tag="LQB69_002550"
     CDS             8884..9258
                     /locus_tag="LQB69_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HBL3526680.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3596 a         1413 c         1455 g         2852 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat ttttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatacccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atacaaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga gaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgagg actctcctcc
     1441 tcaagctcct tataaaaatc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcactaa
     3301 atacgtaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt tttaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt tcaggttgat gtcgccttta ttgattttac
     5761 ttcttgtttc ggttattcaa ccaataactg aataaagaga cacaaactcc tacaagaagg
     5821 gggcaaatga acagttcaaa cattagatac acctccttcc agtcgcaaga ctgtcggata
     5881 ggcgacaagt tattatatct taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg
     5941 ttttagggtt gttagggctt gccctgaccg cctgtaaggc gcttctcgac tagattaagg
     6001 gaaattaatc aagaattgtc ttagaacggc ttagacaagc tttcagagtc tcacacctcc
     6061 tctttgaaca acggttttag caaaggagcc ggaggcgact gcgcagtata atatgagagc
     6121 atagcgatca caccttttta gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg
     6181 cgaacctttt tggaggaaaa agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa
     6241 gatatactgt tagatatact gttagatata ctgttaataa taactgttag ggattagcaa
     6301 ccttatggga gagtaagtat tgacataggt agtgggggat aaattgtcac actatggggg
     6361 ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat tgtcacacta
     6421 tgggggataa attgtgtttt acatcctctt atgtctatga taacataatt tattgaggtg
     6481 atttttattg agtaacgata tagttcgata taataatgga ttaaattctg tccctttaag
     6541 aaaatttacc cctatagata tggatttatt ttggggaata tgttctaaaa tgaaaagaaa
     6601 aggtacaaaa gaagaaattt ttacatttaa acaaataaaa gaattaattg attacaatat
     6661 aaatgcacgt ggcgaccgtt ttgctaatga tttaataaaa atgtcaaaaa aaatcattac
     6721 tttgtctttt atttatgaag acgaaaaaac atatgaacaa ttaaatctgt ttcaaaaatt
     6781 taaaatcgat aaagtgaatg aaacattatt aataaaggtt tctgaagaat ttgaatatat
     6841 acttaattca atcggaacta attttactcg tttcgaatta gaaaatatga caaacttaaa
     6901 gagtagctac gttaaagagt tatatcgcca acttatggct cataaagaca gaagatctaa
     6961 aaaaggtcac tggtttgtaa aaatagaaga ttttagagct ctattagcaa taccagaaag
     7021 ttatcgcatg agagatattg ataaacaaat ttttgcccaa gcgaaaaaag aatttttaaa
     7081 cgttaattca actcaccctg ctatttttaa aacttttgaa atcaaaaaaa ttaaagccta
     7141 caaaggaaat aagataggat ctctgcaaat ttattttgaa gaaaatatac cttcaattac
     7201 cttagataat tggttagaga aataatacct aacaatttag gaggcaatta tcatgcaacc
     7261 caaagaaacc tttacaataa aggaattatc tgaattattt gaaataagta ggcaagcaat
     7321 ctctaaacat gttgataaat tagacccgtc cttattagct aaaaatgagc gtggttataa
     7381 ggttgtatta cgttctggag tattgcaact tgctagcaac ttaggtaacc aaaggttact
     7441 atataggttg catgagtatg aacaagaaaa cgataaaaac gtttatatat caagcaaaaa
     7501 cgtgattgta aacgagttgc tagataagtt gcgtgaagaa aatgaatttt tacgagaaca
     7561 actttccctc aaagagcaaa ataattctaa tatgcaaaaa ttactcgacc agcaacaaca
     7621 gctcacccta caggcaaatc aacagatcga aaaactgcaa gaacagttac aattagctta
     7681 tacggaagaa actccagaag atgaatcaac tactattctt tctgaagaaa cagaatcgat
     7741 agaaaaacac acaaaaaaga agaagcaaaa atggtggcaa ctttggaaat aactggaggg
     7801 aacattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa ttctaggctt
     7861 cagtccgatg ttattgtcag taatcaataa agggaataaa catggtgaat tgaaattgaa
     7921 tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagagacgg ataaataata
     7981 aaaaagactg ctcttccgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag
     8041 cacgcgctta tgaagcgcta ccaaaaattt accatatttt acaaaaaata acaagtctta
     8101 aaaaaatgac agataaaaat gcacaaagaa ttagatatag ataaccgcga aaaatcaagc
     8161 tttagaaagt tgcttttagc tagaccaatt aaaaatcaca aagtgagcgt ctctatccat
     8221 tggtagaggg gctacttggc atagtgtaca ttatgtcaac tatgcaatta aataactaac
     8281 tacttcatat tagcttaata aaataatcaa aataactagg gggaaaaaca ttgtataaaa
     8341 aaatttttca ttttttaaat aagaatagta aatataaaaa taatttaaga tatctattac
     8401 tattaatcca gatagagcaa accaagtaaa tattccatgt gtaaagctaa aaaaattcat
     8461 ttgaatcaat tcaaataaac ctaatatagt aacaaatata ttaaaaataa tagctgtcaa
     8521 taccagtaaa ttattttttt tattttttga attatcttca tttaaaatag aatttatgtt
     8581 agtactataa aattcacaaa ttttttttag tgtctctata tctggtgttg ttttgttatt
     8641 ttcccacttt gaaatagttt gacgtgtaac gttaaatttt cttgccacat attcttgagt
     8701 ataccctttt ttcactctta attctcttag cttagatcct aaatcctcca ttttttctcc
     8761 tatgtaaacg attatttaca taaaatttta tcataaaaat actaacgcgc caaaaaagtt
     8821 ctttatttta taaaacacac taaaataaaa taattctagt aaaaaagata ggagaaataa
     8881 gttatgaaaa aatttttatg tctttctgct ttaatgggtg tcttattaac tagtggagga
     8941 atagctagtg ccacagaagc tttaaatcta gatgtacccg aagaccatac cgccatttac
     9001 ggaggaggaa tggaagctac agaagaagga ttttcttcta gtaaattgag atatgctgct
     9061 ggcggcgggg atttcaactg tggggttaat ggatttaaag tttatgcaaa ttactaccat
     9121 gctagagcta aacatagtgc aacagctaaa aatggacgag gcggtcaagt acgatcagtt
     9181 cagaaagctg gagtaagagc ttatgcaaca tgcaatgcca cacttactgg aaatactggt
     9241 tggtggaatg tttattaaaa aagagagttt aaactctctt ttttatttac atttgaaagg
     9301 aaattcaaaa tatgtt
//
LOCUS       DAFFFQ010000095         9208 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000095
VERSION     DAFFFQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.096"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            470..2107
                     /locus_tag="LQB69_002551"
     CDS             470..2107
                     /locus_tag="LQB69_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526681.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LQB69_002552"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="LQB69_002552"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL3526682.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="LQB69_002553"
     CDS             complement(3183..3818)
                     /locus_tag="LQB69_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL3526683.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LQB69_002554"
     CDS             complement(3965..5206)
                     /gene="sstT"
                     /locus_tag="LQB69_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL3526684.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5404..6400
                     /locus_tag="LQB69_002555"
                     /pseudo
     CDS             5404..6400
                     /locus_tag="LQB69_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            6416..8434
                     /locus_tag="LQB69_002556"
     CDS             6416..8434
                     /locus_tag="LQB69_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL3526685.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8494..9186)
                     /locus_tag="LQB69_002557"
     CDS             complement(8494..9186)
                     /locus_tag="LQB69_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL3526686.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT         2977 a         1706 c         1445 g         3080 t
ORIGIN      
        1 actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac ttaatattaa
       61 gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag gataaaaata
      121 gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt caaaaataga
      181 agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat aaatttcttg
      241 agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa ctactaaaag
      301 aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt ctattttcag
      361 tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcatag cactagtatg
      421 atatactaga aaagaatcaa tttttattac ttttaaatag gagaaaaaaa tgaaaaaaaa
      481 caaaaaccaa ataatagata tcatttttat gttatttcta agttttgctt acgtcatccc
      541 tctattcatg tctatgggaa tctatcatag tgtcaatcag gatacttact ttcatttatc
      601 tcgaatcata ggactagata atgtatggag ttctccagta aacttcaata acttcgatca
      661 tcatggaaca atgatgaata ttttttatcc atggcttact ctttatccag cttttttatt
      721 ttataaaatg atgggaaatc ttgttttaag ttataatatc tactattttt ttatcacttt
      781 tttaacaatg gtcgtttctt atttttctat gaaacaaata aaaaacaatc gttatatttc
      841 gctgttattt tcgattattt atacattttc cgcctatcgt gccattgaca tattccatcg
      901 tgcttcattg ggagaagctg tcgctttaac atttttgcct ttgatactaa tggggtgtta
      961 cgaaatttat attagagatt accaaaaatg gtactggttg tctattggca tgacgctagt
     1021 agtgtatact catttacttt ctgtcgctat ggtttctgta tttattggag gaacgttgtt
     1081 tctttctttt tatttttggg accaaaaaat cgctagactc ttaagtcttt taaaagctac
     1141 cgtcctcacc ttttttttaa gtgcgggatt cttaattcct tttattcaac aatctagagc
     1201 acaagaattg aaagtcccac tgggaaaaga attaagtggt atggctccta gtgatatgct
     1261 gacacacatt ttaaataata actataacaa ctatactatt ggattattct tatttctcgg
     1321 attgattggt gcttttattt ttataaaaaa attaactgct gatgatttat ttattttctt
     1381 tcttggtgta tttgttcttt tttgttcaac caatttattt ccttggcaac tttttaacca
     1441 tacaccagtt aagagtctac aatttgtctg gcggttaaat ggtttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataaacgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagcg attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatgc tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacaggttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaataaaaga ctaacaaata aactgagcaa ggggttgtga
     8461 taaaagacat gtcacaaccc cttgtttatc tcattaattt gcttgtccat aaacgatttg
     8521 attaaagcca tttttcaatt cattattcgc tgtctcgttg aagtaaagat aattatcggg
     8581 attcccctga ttttgttgtg tcaccaatac ttttggctga ttttgctgaa acccgaatag
     8641 atagacgtgc tttttcagat atggttctgt ctctgcatcg gaatagacgg ctgtgatttg
     8701 atagtctgct tgacctgtcc cactttctga ccattctgca gaaacaggca ctccaccaat
     8761 agccatcttc cactccccgt tcaaaatagc ttgagggact ttaagaccat ataagtccac
     8821 actcatttga ttcgtataac ttttgtattg ttgtcccatt gtttggcccc atttggtcat
     8881 aaatgcctct aattgtgcgg atttgtctgc attccacagt gtttgttcaa cttgttcgct
     8941 gctttctgaa gaggaagatg aagcctctac agaagaactt gaagtagatg tttgagttgt
     9001 ttcagtcgtt ttggaagact ctgacgaagt agaggtatct gctgtttctg tacttgctgc
     9061 agttgacttg ctctctgtgc tagttgttgt agtagttgca actgttgctt tcgctgtttc
     9121 ttttgttgat tcttttttcg attcattccc gttggtacaa ccagctaaca aaaaagctag
     9181 accaattgtt actaaaatcc tttttttc
//
LOCUS       DAFFFQ010000096         9154 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000096
VERSION     DAFFFQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.097"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            1..267
                     /locus_tag="LQB69_002558"
                     /pseudo
     CDS             1..267
                     /locus_tag="LQB69_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            806..1006
                     /locus_tag="LQB69_002559"
                     /pseudo
     CDS             806..1006
                     /locus_tag="LQB69_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="LQB69_002560"
     CDS             1493..1672
                     /locus_tag="LQB69_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526687.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="LQB69_002561"
     CDS             complement(1718..2323)
                     /locus_tag="LQB69_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526688.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="LQB69_002562"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="LQB69_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3005..3232
                     /locus_tag="LQB69_002563"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="LQB69_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="LQB69_002564"
     CDS             3358..4329
                     /locus_tag="LQB69_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3526689.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="LQB69_002565"
     CDS             4469..5782
                     /locus_tag="LQB69_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL3526690.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="LQB69_002566"
     CDS             5825..6691
                     /locus_tag="LQB69_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL3526691.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="LQB69_002567"
     CDS             6688..7536
                     /locus_tag="LQB69_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL3526692.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="LQB69_002568"
     CDS             7550..9022
                     /locus_tag="LQB69_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL3526693.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9154
                     /locus_tag="LQB69_002569"
     CDS             9039..>9154
                     /locus_tag="LQB69_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3526694.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1525 c         1871 g         2838 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caat
//
LOCUS       DAFFFQ010000097         8969 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000097
VERSION     DAFFFQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.098"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(57..350)
                     /locus_tag="LQB69_002570"
     CDS             complement(57..350)
                     /locus_tag="LQB69_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL3526695.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="LQB69_002571"
     CDS             complement(378..1043)
                     /locus_tag="LQB69_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL3526696.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="LQB69_002572"
     CDS             complement(1183..1815)
                     /locus_tag="LQB69_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526697.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="LQB69_002573"
     CDS             complement(1822..2889)
                     /locus_tag="LQB69_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL3526698.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="LQB69_002574"
     CDS             complement(2886..3617)
                     /locus_tag="LQB69_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL3526699.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LQB69_002575"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LQB69_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL3526700.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LQB69_002576"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LQB69_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL3526701.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5780..6949
                     /locus_tag="LQB69_002577"
     CDS             5780..6949
                     /locus_tag="LQB69_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL3526702.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(7216..8850)
                     /locus_tag="LQB69_002578"
     CDS             complement(7216..8850)
                     /locus_tag="LQB69_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526703.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         2856 a         1701 c         1333 g         3079 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt actataagat
     5701 taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt aaaaaagaaa
     5761 ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa aatattcatt
     5821 tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa ctattcttgg
     5881 atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat cagctcaaaa
     5941 aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc aactattaag
     6001 aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca tcatggaaaa
     6061 aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc tttattatca
     6121 gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag tcgagcgtgg
     6181 gcacctttct ttagatacta agctggctca attctatcca caaattgctg gcagccagca
     6241 aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg cttctccaac
     6301 cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa aatttacaga
     6361 ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa ttttgatcaa
     6421 attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac ctttaggatt
     6481 acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac tatcctatca
     6541 aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag agatccataa
     6601 cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc tgtttacaca
     6661 agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc gtgaaaatgc
     6721 gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc tgtcgcaagg
     6781 aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg atgctatcat
     6841 catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg atctaagaaa
     6901 ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat atgatgaaaa
     6961 gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg actaaacgta
     7021 ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa ttccaaataa
     7081 acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa gatttctctt
     7141 taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta gattgctaaa
     7201 atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc aataaaaaag
     7261 aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa atgtgtatga
     7321 atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa aaggacctcc
     7381 ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt tataccggaa
     7441 tacaggtaaa actattgttt tgccttttga agatttaagc gtaaatgtaa tttcatcttt
     7501 atttggagaa aagctactat aaataacttg accatcttct gtaacttgat ggtttttcaa
     7561 aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa attcttcaag
     7621 tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt tcaccatgtt
     7681 aactactgat gaataattta aaattgaaat agctaatatt aataatagta taaaatttct
     7741 tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata ctgaaaaaga
     7801 tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat ttaaaggagt
     7861 attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata ttatacccat
     7921 tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta gtataattaa
     7981 acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta tacttccaat
     8041 caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat aaggtctatt
     8101 cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg aaagaagtaa
     8161 agtaactata gcagctttta ttaatttaat aattaagaag atttctattt tctttttccg
     8221 ataaaattga ataattaaag caattataat cattaccata gtcatagcta cagaaagaac
     8281 atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt ctttttttga
     8341 gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg cgagtaattc
     8401 ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg tatatataat
     8461 agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat aatgacatat
     8521 aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg ctaaattagt
     8581 aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat tatttacttg
     8641 catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag attctaaact
     8701 taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca tgtttttaaa
     8761 tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt ttacagaaaa
     8821 aatttttttc aaattcatat accacctcat gtttttttat attacatata gtatcattta
     8881 tttttttaaa acactagatt agtcactata taaaatgtga ttaattttat tattaataaa
     8941 atatttttta tattacaaaa gtagtgttt
//
LOCUS       DAFFFQ010000098         8731 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000098
VERSION     DAFFFQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.100"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            2..74
                     /locus_tag="LQB69_002579"
     tRNA            2..74
                     /locus_tag="LQB69_002579"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="LQB69_002580"
     tRNA            78..150
                     /locus_tag="LQB69_002580"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="LQB69_002581"
     tRNA            155..225
                     /locus_tag="LQB69_002581"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="LQB69_002582"
     tRNA            238..311
                     /locus_tag="LQB69_002582"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="LQB69_002583"
     tRNA            323..394
                     /locus_tag="LQB69_002583"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="LQB69_002584"
     CDS             660..1853
                     /locus_tag="LQB69_002584"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL3526704.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="LQB69_002585"
     CDS             1983..3455
                     /locus_tag="LQB69_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3526705.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="LQB69_002586"
     CDS             3538..4326
                     /locus_tag="LQB69_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL3526706.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="LQB69_002587"
     CDS             complement(4393..5061)
                     /locus_tag="LQB69_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL3526707.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="LQB69_002588"
     CDS             5176..6888
                     /locus_tag="LQB69_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526708.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="LQB69_002589"
     CDS             6891..8663
                     /locus_tag="LQB69_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526709.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAFFFQ010000099         8708 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000099
VERSION     DAFFFQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.101"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            7..2199
                     /locus_tag="LQB69_002590"
     CDS             7..2199
                     /locus_tag="LQB69_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL3526710.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            2216..2692
                     /gene="ybeY"
                     /locus_tag="LQB69_002591"
     CDS             2216..2692
                     /gene="ybeY"
                     /locus_tag="LQB69_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL3526711.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            2676..3071
                     /locus_tag="LQB69_002592"
     CDS             2676..3071
                     /locus_tag="LQB69_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL3526712.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            3086..3985
                     /gene="era"
                     /locus_tag="LQB69_002593"
     CDS             3086..3985
                     /gene="era"
                     /locus_tag="LQB69_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL3526713.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            4128..4940
                     /gene="recO"
                     /locus_tag="LQB69_002594"
     CDS             4128..4940
                     /gene="recO"
                     /locus_tag="LQB69_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL3526714.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            5324..6241
                     /gene="glyQ"
                     /locus_tag="LQB69_002595"
     CDS             5324..6241
                     /gene="glyQ"
                     /locus_tag="LQB69_002595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL3526715.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            6243..8318
                     /locus_tag="LQB69_002596"
     CDS             6243..8318
                     /locus_tag="LQB69_002596"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL3526716.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         2936 a         1559 c         1849 g         2364 t
ORIGIN      
        1 aagcacatgt tgaataaaac actgcagaaa ctacaagaaa aacttggaaa ggcagcattg
       61 cctttcctgc tgttcttgtt ttttatcatt atctgcggcg ttacatttag tagtgtcaaa
      121 caaaaggcca atgatttcag agaaggccaa gtagcagaag aaagtatccg tgcgaacaaa
      181 acgatcgaga acacggaaga aaccgaacaa aaaagaaaac tggcagcaga agcagtcact
      241 ccagaataca cttaccaaaa agatcttgca gatgaccaga acaatcggat caagcaacta
      301 tttgaattga tcgataaaac gaatgatgcc atcaacaaat cttatgacga aaagattgat
      361 aaggcaaagg aagatgaaaa tatcccaaag cctactacag aagagcggat cgccgcgttg
      421 aagaaaaatt ttgaaaatgt gaatgcagaa aatgttgcgt tttatcaaaa actaccgaac
      481 aatttttatt cgacgatatt tgaaatgact gaaactgaga tccatactgt gagggatgag
      541 agtctgaagc tcattgacga acagatgagc aaacaagttc gtgaaagtga attggaagct
      601 tttaaacaag aagcagaaga gcaaatccaa tatttgaatg tcacaccagc acaacaacaa
      661 atgattcgct acttagtgga tcaaggtatc gtagtgaatg acgtattgaa tgagaaaaag
      721 acagaagaat tgaaacaatc cgcacgagaa gctgtacaac cagtgatgat cttccaagga
      781 gaaatcattg ttcgtgaagg taatcaaatc gatgcttcag ctatgaaaaa attggaatta
      841 ctaggactga ctaatcaaac aacttcgatt tttccgctaa ttgccatggt tttagctgtt
      901 ttgcttcaaa tcggcgtact gatttataat tcacttcaat tccaaaatgc aggagaaaga
      961 acaaaatacg tgcttttcta tgtgacagct atgtcagtca gcatagtgct gatgaaattt
     1021 ttccaattat tccaaacgga acaagctact tatatcccgc tgttttatcc cgctgctttt
     1081 gcaccacttg ttttaagctt tttcttgaac cgaagagcag ggatcatggc tggtattttc
     1141 caagcagtat ctgcactttt cattttttat gattcgattg gcacgaactt tctaacgatt
     1201 atcttgatgt cttatgtttt ctctgggctc atggcaacga ttgtcagacg caaacggatc
     1261 tcagaacaag gattatctgc tgccatgtgg gtcattattt ttcctgtgat gatggatgtc
     1321 attttgatcg tctatcaagg aatgagcttt ggtgatgcaa cgacttggtt aatgcttgtc
     1381 tgcgggttca ctggtgcact cttctcattc ttgctgacga tcggtctaca cccatatatt
     1441 gaattgatgg tcaatgatga cagtgtaatt gtattaaatg aactcagcaa tccaaatcat
     1501 cctttattga aacagttgct ggaagaagcg ccaggtactt atcatcatag tatgatggta
     1561 gctaatctaa gtgctaacgc tgtagcagaa atcggcggac gttctttgct aactcgagta
     1621 gcttgctatt atcatgatat cggcaagatc aaacatgcta gctttttcgt tgagaacttg
     1681 ccttcaggag cggaaaaccc gcacaatttc ttattgccag aagacagtaa acatattatc
     1741 tttggacatg tgacagatgg agccaaaata ttggaagatt ataatatgcc gcaaatggtg
     1801 atcgatattt gcaaacagca tcatggaacc acgctaatgc gttatttcta catcaaagcg
     1861 aaagaacgca atccggaagt aacagaagag caattccgtt atcctggacc aaaaccgcaa
     1921 agtcgtgaag cagggatcgt caatattgct gacagttgtg aggctgcagt acgggcaatg
     1981 gatcatccga cgagcgaaaa aatcgaaagt tttgtccata atcttatcga agagcgtatt
     2041 tccgatggtc aattagacga tagtggactg accttgaaag aaatccgaaa agtggaaaaa
     2101 tcattgatca gcggtttaag tagcaccttc cattctcgga tcaaatatcc taaaatgaaa
     2161 acagaagttg agaagataaa ggaagaacaa gaagaataga aagaagcgat gtataatgga
     2221 tattaccttt atggatgaaa caaaccaagt ctcagaagag aaaatgaaag aaatcgatga
     2281 cttgctgcaa tttgcagcat cttatttaaa gctgccagag gaaacagaaa tgtctgttac
     2341 tttcatggac aatgcggcaa tccaagtaat caatcgtgat taccgtggaa aagatgcacc
     2401 gacagacgtc atcagttttg ctctggaaga agaaggcgaa gacgaaatcc cagtgatttt
     2461 tgaagatgga gagaatccat tgcctcgtgt actaggagac atcatgattt ctattgaacg
     2521 tgctgctgaa caagcagaag cttacggcca ttcatatgat cgggaacttg gctttttagc
     2581 ggtacatgga ttcttgcata tcaacgggta tgaccatatg acaccagaag atgagaaaga
     2641 aatgtttggt ttacagaaag agatcttgga tgcctatgga ctcaaaagat aaaaacgtag
     2701 agaaaaacaa acatttcctg atgtctgtgg aatttgctgt ccaaggagtc aaaaccgttt
     2761 ttgaggaaga acgcaatatg aggaaacatg ttgcgttcgg tatccttgct ttatttatgg
     2821 gcgtgatttt ccagcttgat cggatagagt ggttatggct gcttttagct gtttttctcg
     2881 tttggatagt agagatcatc aatactgttt ttgaaaatgt agtggacatg tttacggatt
     2941 ttcattttca tccgattggg aaaaaaatca aagatatggc agctggagca gttttagtga
     3001 ccgcttgttt tgctgtcatc attgggttga ttttatttgt ccctaaaata tatcatttgt
     3061 tgtttaatta aaaaggagaa cgtatatgcc agaacataaa tcagggtttg tcgcaatcgt
     3121 cggccgcccg aatgtaggaa aatccacatt attgaaccga atcgtgggtc aaaaaattgc
     3181 cattatgagt gacaaggcac agacaactag aaataaaatc caaggagtat atactacacc
     3241 agaagctcag ctgattttta tcgatacacc aggaatccat aaaccaaagc atcgtctagg
     3301 agattttatg gtagaagcag cttacagtgc gttgaaagaa gtggatgcag tcttatttat
     3361 gatcagtgct gatcaaaaac gcggccgggg agacgatttt attattgaac gtctaaaaaa
     3421 cgtgcattct ccagtttatc tgattatcaa taaaatcgat aagatccatc cagacgagtt
     3481 gctgggaatc atcgaagatt attcgacgca gatgcctttt gcacaagtga ttccgatctc
     3541 tgctacagaa ggaaacaatg tcgaacgctt aatggatgta ttagtcagtg aaatgccaga
     3601 gggcccacaa tattttccag atgatcaagt gactgatcat cctgaatact ttatcgtttc
     3661 tgaattgatc cgtgagaaag tcttgttttt aacacgagac gaagtgcctc attctgtcgc
     3721 agtcgttgtt gattcgatga agcgaaacga gaatgataaa atccatatcc aggccacgat
     3781 catcgtcgaa agagatagcc aaaaaggaat cattatcggc aaaggcggaa aaatgctgaa
     3841 gcaagtagga acgaaagcaa gaaaggatat cgaaaatctt ttaggagaca aagtatttct
     3901 agaactttgg gtcaaagtcc aaaaagactg gcgagacaaa aaagtctatc tgcaagactt
     3961 tggctaccga aaagacgaat actgatattc tgaacttctt ataggttaag tatagaaata
     4021 aactggataa aaatccgcac gttgagaaaa acggcggaag atcgaaacat aaataatctg
     4081 gatcggcaga atctgctttt ccagatttat tttttaggag ggagaatatg agccagcttg
     4141 cagaatcgaa aggcttgatt ttatctagcc gtaattacaa agaaaaagat aaattagtca
     4201 aaatctttac ggaatcttct ggcaaaatga tgttttatat caaaggaatc catcggcaaa
     4261 atcagccatt agctccggct ttgctcccct ttaccgaagc agtctatatc ggtaattttc
     4321 gaagtgaagg tttgtcgttt ttaagcagtg tcaaagacgt ccatgcgttt cggaacatcc
     4381 aagaagatat ctttttgagc ggttatggta cctatttgct aaatctagta gatgcagcaa
     4441 tcgaagatca tcaatatgac ccgcatttat tccaatttac gcaacaagct ctgcagcgga
     4501 tggatcaagg agatgatgca gaaatcatca cgaatatctt tgaagttcaa atactgcaac
     4561 gcttcggaat cgcgccaatt tggacgcact gtgtcgtatg cggggaaaca aaaggaaaat
     4621 ttgactattc ttccaaatct ggcggtgtga tatgcgagaa acactggcca atggacgaac
     4681 accgttatca tgcagatcct cgcgctgttt atttcgttcg aatgttttcg gctatttctt
     4741 atgataagat cagtggaatc cacttgaaag aagaaacgaa gcaggcaatc cgtgacctca
     4801 ttgaccagtt gtatgatgaa tatgtcggta ttcatctaaa aagcaaaaag ttcattgatc
     4861 aaatgaagtc ttgggcacac gtattaaaga cgccagagaa aaatatgcct gaaaaagaga
     4921 tacctgaaaa agaagaataa atgaaagata caacaaaaaa atcaaaacta tttgacaaat
     4981 acagggactt ccgtctatta ttaaccgtag attaaataga tcgttcactg agaggatcta
     5041 ttatataaac aagaaaactg caaaatgaat acgtacattg accaagatca gtaaaaaaca
     5101 cgtggtttag cgagttcggg agagtggaag ccggacccaa acgatttttg aagaggcact
     5161 tgcgagtacg ataaatgaag ctgccgaatc tttattcgga agtagggtgg aaccgcgaaa
     5221 ttattcgtcc ctatgtctgt aatcagacat agggactttt tgtcgttttc tttcctgtat
     5281 tcttattgac agcaacgctt aaaaattgaa aggggcaaaa cagatgagta aaaaattgac
     5341 cgtccaagat atgattttaa cattacaaaa attttggtca tcaaacggct gcatgctgat
     5401 gcaagcttat gacacagaaa aaggagcagg tacgatgagc ccatatacat tcttacgggc
     5461 aatcggaccg gaaccatgga atgctgctta tgtagagcca tcccgccgac cagctgacgg
     5521 gcgttatgga gaaaacccaa accgtttgta tcagcatcat caatttcaag tcgtaatgaa
     5581 accttcacct gaaaatatcc aagagcttta tttagaaagc ttgcgtcttt taggaattga
     5641 tccattagaa cacgatattc gttttgttga agacaactgg gagaacccat cgatggggtg
     5701 tgccggtgtg ggttgggaag tatggcttga tgggatggag atcacacaat tcacgtactt
     5761 ccagcaagtc ggaggactac cttgtaaacc agttacttct gagatcactt atggactaga
     5821 acgtcttgct tcatatattc aagaagttga aagtgtctat gatctggaat gggctgatgg
     5881 tgtgaaatat ggcgagatct tcaaacaacc agaatatgaa cattccaaat attcttttga
     5941 agtaagtgac caagacttgc tactaagcaa ctttgaccgt tttgaaaaag aagctaaacg
     6001 ctgtatcgat gaaaatcttg ttcatccagc gtatgattat attttgaaat gcagccatac
     6061 attcaattta ttagatgctc gcggtgcagt atccgtaaca gaacgagcag gttatctttc
     6121 ccgtatccgc aacatggctc gtgctgtagc caaaatcttt gtggcagaac gcgaaaaatt
     6181 aggattccct ttattgaata aaaacacagc aaatccagaa aacatgaagg aggctgaata
     6241 agatggcaaa agacttatta cttgaaatcg ggctggaaga aatgcctgct catgttgtca
     6301 caccaagcat gaaacaatta gaagaaaaaa cagcaaaatt tttagatgaa aaccaattga
     6361 cttatgatac gatcgaaacg ttttctacac cgcgccgctt agcaatcaaa gtaacaaaca
     6421 ttccagaacg tcaagcagac agtgaagaag aagtcaaagg accagccaaa aaaattgctt
     6481 tggacgcaga tggaaactgg acgaaagcag ctgaaggatt tgtccgcgga caaggtctga
     6541 cgacagatgc tatcactttt agagaattaa aaggcgtgga atatgtctat gtaacgaaat
     6601 acactcacgg caaagaagcg aaagaagtat tgacaggttt agcagatgtc attaaaagcc
     6661 tcactttccc agtaacgatg cattgggcag aatacgattt tgaatatatc cgccctattc
     6721 actggatcgt ggcattgtta gatgacgaag taatcccgtt tgagatctta gatgtcaaaa
     6781 caggcagagc ctctcgagga catcgtttct taggcgaaga tgtgacattt gcgcatgcaa
     6841 aagaatatga agaaaaattg aatgaacaat tcgttattgc ttcaccgcaa aaaagacaag
     6901 aaatgatcgt tacacaagca aatgaattag ccgaaaaaaa tcactgggtt attgatctag
     6961 acgaagattt actagaagaa gtcaacaatt tagtcgaata tccaactgtc ttttctggcg
     7021 acttcgaaga aaaatatctt tctgttcctg aagaagtact agtcacttct atgaaagaac
     7081 atcaacgtta tttcgaggta cggaacgatc aaggtatgct gcttcctcat tttatttctg
     7141 ctcgtaatgg tgatcgggta aaattggaaa atgtggttcg aggcaatcaa aaagtattga
     7201 cggctcgttt agaagatgcg gaattctttt ataatgaaga caagaaagct tcaatcgacc
     7261 aatacgttga aaaactaaaa actgttactt tccatgaaaa aatcggtagt atctatgaga
     7321 aaatgcagcg ggtacaagtc attgcccgta tcattggcga acaagtagga ctatctgagg
     7381 aagaattgat ggatctgcaa cgagctgctg aaatctataa atttgatcta gtaaccaaca
     7441 tggtagggga atttcctgaa ctacaaggga tcatgggcga aaaatatgct ctgctgaaag
     7501 gcgagaaacc tgctgtagca acagcgattc gtgagcatta catgcctata tccagtgaag
     7561 gagagctacc tcagtctaca gtaggttctg ttttagcgat tgcagataaa ttagatagca
     7621 ttttttcttt ctttgcagtt gggatgattc caagcgggtc aaacgatcct tatgccttac
     7681 gtcgccaagc atacggtgtc gttcggatca tcgaatcgaa aaaatgggcc tttcctttgt
     7741 ctgttctgca agaaacgatt agtgatgtta tcagtaaaga tacggatcgt ttcggcattg
     7801 gtttatcaga agggcaacaa caggtgatcg actttatcaa aggccgtctg cgccaattat
     7861 tgacaacgaa gaatattcgt catgatgtca tcgaagctgt tttgaatgca gaacaaaaag
     7921 atctgacaaa agtatttgca gcagcacaat tgttcaagca gcatcttgca gatgaagact
     7981 tcaaaccatc gatggaagct ttgacacgtg tcgtgaactt agcgaaaaaa gcggagcttg
     8041 aaaaacaatc agaagtagac cctgaactgt tcgaaaatga agcggagaaa gaactgcaca
     8101 aagcagtaga ggcaatgaaa aaaagctttg ctgaccaaac gatcaacgaa aattatgatc
     8161 agcttgtaag ccttcgccca ctgatcgaaa attattttga tcagacgatg gtcatggcag
     8221 aagaaccagc agtacgtaag aaccgattaa cgcaattaag tcaaattgct gccatggctt
     8281 tgtcgctagc aagtttagat caaatcatca caaaataatt tctaatatgt aaataaacca
     8341 gagaataggc acaaaatagg ctatttctct ggtttatttg ttataaatat aaataactat
     8401 acccgtggtg ctagttaatt ttttcctagg tattatacaa gtaatttgag tcggtcaatt
     8461 tcaactgaat tatctaaact aactgatcta cttttagata attcagttga aatttattgg
     8521 taaactgtcg cttttctttt taaatggcaa aaatttttaa taatatatga caatttagaa
     8581 atcaactata agactgatta attcaaaatg atcttagaat cattgtatga acactaaata
     8641 ctatattgtt ttctattaat cgttatataa acgattatga attcaaagct actttttatg
     8701 acgtcaat
//
LOCUS       DAFFFQ010000100         8493 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000100
VERSION     DAFFFQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.102"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..173)
                     /locus_tag="LQB69_002597"
     CDS             complement(<1..173)
                     /locus_tag="LQB69_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3526717.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LQB69_002598"
     CDS             complement(593..1714)
                     /locus_tag="LQB69_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL3526718.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LQB69_002599"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LQB69_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL3526719.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LQB69_002600"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LQB69_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL3526720.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LQB69_002601"
     CDS             complement(4304..5215)
                     /locus_tag="LQB69_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3526721.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LQB69_002602"
     CDS             complement(5232..6239)
                     /locus_tag="LQB69_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL3526722.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LQB69_002603"
     CDS             complement(6236..8365)
                     /locus_tag="LQB69_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL3526723.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="LQB69_002604"
     CDS             complement(8370..>8493)
                     /locus_tag="LQB69_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3526724.1"
                     /translation="TGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1487 g         2679 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg gtt
//
LOCUS       DAFFFQ010000101         8289 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000101
VERSION     DAFFFQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.103"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(44..1510)
                     /locus_tag="LQB69_002605"
     CDS             complement(44..1510)
                     /locus_tag="LQB69_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL3526725.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1897..2493
                     /locus_tag="LQB69_002606"
     CDS             1897..2493
                     /locus_tag="LQB69_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3526726.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2551..3033)
                     /locus_tag="LQB69_002607"
     CDS             complement(2551..3033)
                     /locus_tag="LQB69_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526727.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3139..3336)
                     /locus_tag="LQB69_002608"
     CDS             complement(3139..3336)
                     /locus_tag="LQB69_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526728.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3631..4389
                     /locus_tag="LQB69_002609"
     CDS             3631..4389
                     /locus_tag="LQB69_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL3526729.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4449..4877
                     /locus_tag="LQB69_002610"
     CDS             4449..4877
                     /locus_tag="LQB69_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526730.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4923..4995
                     /locus_tag="LQB69_002611"
     tRNA            4923..4995
                     /locus_tag="LQB69_002611"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4956..4958,aa:Gln,seq:ctg)
     gene            complement(5145..5516)
                     /locus_tag="LQB69_002612"
     CDS             complement(5145..5516)
                     /locus_tag="LQB69_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL3526731.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5731..5916
                     /locus_tag="LQB69_002613"
     CDS             5731..5916
                     /locus_tag="LQB69_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526732.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(5999..6187)
                     /locus_tag="LQB69_002614"
     CDS             complement(5999..6187)
                     /locus_tag="LQB69_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526733.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6211..6474)
                     /locus_tag="LQB69_002615"
     CDS             complement(6211..6474)
                     /locus_tag="LQB69_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526734.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6632..6889
                     /locus_tag="LQB69_002616"
     CDS             6632..6889
                     /locus_tag="LQB69_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL3526735.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6960..7151)
                     /locus_tag="LQB69_002617"
     CDS             complement(6960..7151)
                     /locus_tag="LQB69_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526736.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7292..7612)
                     /locus_tag="LQB69_002618"
     CDS             complement(7292..7612)
                     /locus_tag="LQB69_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526737.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7635..8069)
                     /locus_tag="LQB69_002619"
     CDS             complement(7635..8069)
                     /locus_tag="LQB69_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL3526738.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2627 a         1635 c         1314 g         2713 t
ORIGIN      
        1 ggaactgctg caaataagca attcctgttt ttatgttcat tgattaatga atagagccca
       61 ttagtttttt gattggttct ttcacagtaa atagcaagat accaccgacg attgctactg
      121 caccgataat accgaagtaa gcagattctg ttcccggtgt gtagaagcgt gcaatttgtg
      181 cattgattgc ttgtgaagaa gcatcagcta agaaccaaat agcgattgtt tgtgattcaa
      241 atgctcgtgg tgctaatttt gttgtcacag acaaaccaac tggtgaaaga cacaattcag
      301 caataaccac gatgaagaaa cttcccaaca accacaatgg acttactttt ccacttgtac
      361 cataaagcat acccggcaac atcaatagca agtacgataa tcctgcaaat aaaagtccaa
      421 gagagaattt cactactgta gatggttgac gttcaccaag tttcgtccac atcgttacga
      481 atactggtgt cagaataaag atgaaaattg gattcaatga ttgataccag cttgcttgga
      541 tcgttacacc aaatagcgta gattgtgtac gttcactagc aaataaagct aagatagatg
      601 aaccttgttc ttgtaatgac cagaaaatga ttgctgcaag gaataatggg atataagccc
      661 atactcttgg tttttcttca gcagttacat cttttgaagt cagcattttg ataaagtaat
      721 acaacggtaa gataatcccc aatacactga ttgtattgac aaaaaagtca attgtcagat
      781 gaccagttag ttgtgccccg ccgaatactg cagcgataac aacaagtgcg atgatcaagt
      841 tacggatgaa tcctttacgt tcttcagttg tcaaaggatt cggcggtgtt gtacccactc
      901 cgtctaatgt cttgcgtcct tggatgatgt attgtaataa tccaaagaac atcccaatag
      961 cagctaaaga gaaccctaaa tgatagttat attcttgacc gattgtacca acgattagtg
     1021 gtgctaataa tgcccctaag ttgacaccca tgtaaaaaat cgaaaatcct gcatctcttc
     1081 gcaagtcagt ctttgaataa agatgtccga ccatgccaga aacatttggt ttcaaaaagg
     1141 cggttccgat aataatcaat gccatcgaaa caaacaatgt acttacacca aatggtaaag
     1201 ccaagacgat gtggccaagc ataatgaaaa ctccaccgaa gaatacagtg cgttttgctc
     1261 ctaacactcg gtcagatacc catccgccga cgatactaga catgtagatc aaagatccgt
     1321 agatcgacat gatagctaat gctgttgctc gtggtaatcc aagtcccccg ttggcaactg
     1381 tatcgtacat ataatagata agtaatgcac gcataccgta ataactaaat cgttcccaca
     1441 tctcagtgaa gaacaaagtt gacaatccct tcggctgccc gaagaagccc ttgtcttgct
     1501 gtttttccat aatctatacc ccaattcttt aaattttcag aaaatattat acatctttac
     1561 cgaaaaattt aactaaaaaa cttatttaag aaatataaac taattttcta taaaagcaag
     1621 taatttttaa tggcaatatc ataaaaaaaa gaagatagag atttaataat tatatatcag
     1681 ctgttttttt caaataagca aaaattccta aaacacaaca atgaaagcga taactaagaa
     1741 aatccttaca taacgatttt tcttattaaa attgtttttt gaacttcgct tattttttgt
     1801 gagcatatct gtttacttac tttttgtaag gtgctatact tcatttcgta atcaattaaa
     1861 aaaatgcaaa acgcaaatca aaggagaaat tttattatga caacttttac tgacactttg
     1921 aaaaaccgcc gttcaatcta tcatctagga cgtaatgttt cactttctaa tgaagaatta
     1981 acaactttga tcaaagaagc aattaaagaa agcccaactg ctttcaatgc tcaatcaaca
     2041 cgcgcagtta tccttttcgg agatgcacat gaaaaacttt gggaaatcac tgaagaagca
     2101 cttcgcccat tgactccagc agaagctttc ccaaatacac aaaacaaatt agctggtttc
     2161 aaaaatggtt atggtacagt attattcttc aaagatactg acgtggtaaa aggtctacaa
     2221 gaacaatttg aattatatgc agacaacttc ccagattggt cagaacaatc aaacggaatc
     2281 gcaacagcta atacttgggt agcattagtt gataaaggat tgggcgcaaa cttgcaacat
     2341 tacaacccag ttattgacga agcagttgca aaagaatgga atatcccttc aaactggaaa
     2401 ctacgttcgc aattagtatt cggttcacca gaaacaccgg ctggggaaaa agaatacatg
     2461 aacgatgccg atcgcttccg cgtatttgga taattttatt tgaaataaaa aacagaggtt
     2521 gtgacaaaca tcttgtcgca acctctgttt ttattctgga tcaatcataa tatcaatctc
     2581 tgcctcatcg tttaagcgtt gggacaaaaa tggaactaat tcttctaatg atgcagaaaa
     2641 tttcagcatc ggtcctcgcc atccgtttcg gaaaaaattc atttttacga ttttagaacc
     2701 ttttggctga tataatacaa ttttattgat ctcagaaaaa ggaacacctt ttttgtcaaa
     2761 gctattcaaa acaagtcctt cttctgtgat tcctctactg tccaacaaaa aactaataaa
     2821 aatcattgct gacaagatcc cttttgccaa ttcatctgct gtaggatgac tgatgagtgt
     2881 ataccaaatg actagtgaca ctaagaaggg tatcaccaag tagacccatc gatttcgcac
     2941 agtccgataa ataatttctt ttcgaaccag ccaaagaaat aaaaacatca caacaacgaa
     3001 taaaaacaat agaaccgtaa agaatgttac catgacaact cgcctcgctt tttcttctcc
     3061 agaaaatagg aaaagaatac tgatttacct tctcaaagaa attggacttg cagcacgccg
     3121 cacgtccaat tgttttcttt attcaaatgt atcatatttt gcaacaacca tgcttgcttt
     3181 ttcatctaat aattcttggt cctctgcagc acattgggca actttgacca cggcgcagtt
     3241 tgtcattttt gatacaactt caccaatgac cgtttttgta aaaccaattg gtttgcatgc
     3301 atattgttct cctaaattaa tcatttcttc cgtcatcaaa atcctcctaa actgctggaa
     3361 cttttctcca cattaccact gctatttctt tggagattta actcattgat ttttatttac
     3421 agttaaaaat caaccttaca ggcattatag cgtactgttt tcgttttcac aattaaaaca
     3481 gctgttgtat ttagaagaat ttttttcaaa aaattaaaac atattcctta aaaactaatt
     3541 atttcctaca tattaaaata taatacttcc atttccaata aaaatacatt tataatagga
     3601 aagataaaca gctgaaaagg gagatttcaa atgaagaaaa aaacgttcaa aaaactattg
     3661 tttctagggg gcacagctgc tcttttatta agcggtacaa gttattacct acatatccat
     3721 tctccagaac atacaatgaa cactgcacaa aaaagttcaa cacaaaaaac gaaaaagaca
     3781 aaagagaaag cagaagtttt actagatgtt ccattagaga gccagtttga ttctccttct
     3841 ttagaaaatg gctgtgaagt cacttcgctt tctatgcttt tatcttttta tggttatgaa
     3901 acaactaaaa accagttagc tgagcaattg gattacgtcc ctgttttcaa tgcagatggg
     3961 actcatggcg atcctaatga aggatttgtt ggtgatatca gtggcggaga ctgggctatg
     4021 gtcgtctatg ttcccccagt tgcctccctt gctcagcaaa tcgtccaaac ggattatcac
     4081 acttttccaa aaaccgatgc tcaactcgat gatatcaaaa aagcactatc taaaggaaat
     4141 cctgtctgga cttctgtgac tatcaatttt gaagtcccag atgaaactga ttttatgact
     4201 tggaccacaa ataatggaga agtaagagtc actcctcttg ttcatgcttg tgtagtcact
     4261 ggatatgaca aagaaaatat ttatgtcaac gatccctatg gtgtaaaaaa tcgagcagta
     4321 ccgatcaacg atttttcggc gattttcact gcaatgggtg gacagatgct aactttggag
     4381 aaaagctagt aattcaaaca aaaatccctt atactcaaga taccaaacct ttgaagggag
     4441 cgtagataat ggaaaaattc acacataaaa aaatggatcc aaatgaaatt ccgattattt
     4501 ttgtccgtga ctgcaaggga aatgttcaag gtaaagtctc tattaatgag tggaacgagc
     4561 gtcgcagacc tgctaccctc aatgaactgg aaatcaaatt gtatcgtcaa tctcttgttt
     4621 attacgcaga tcaagagtac gagaaagcca ccgatttatt gaaatttcta attgctcgaa
     4681 cagagtatac tcgctttgaa tatattgaac gtttagcaaa catctatcat attatgaatg
     4741 aacctgtcaa agaatatcag ttattggaca ccgtcttgtc agtagcagaa ctgattgccc
     4801 ttcctgctgg attagaaaag aaactagtac gccgcctttt acgagtaaag caacaactat
     4861 ccgatcaaga aaaataactt gcgttttttt aggaaagtgc tatgattatt ttgttgaata
     4921 aatgggatgt agccaaactg gtaaggcact tgactctgaa tcaagcaatt gtaggttcga
     4981 accctgccat cccaatttga tgtgcttgct gaaaaaatga accccctgta aaagaacaat
     5041 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
     5101 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
     5161 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
     5221 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
     5281 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
     5341 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
     5401 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
     5461 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
     5521 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
     5581 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
     5641 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
     5701 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
     5761 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
     5821 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
     5881 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
     5941 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
     6001 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     6061 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     6121 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     6181 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     6241 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     6301 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     6361 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     6421 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     6481 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     6541 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     6601 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     6661 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     6721 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     6781 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     6841 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     6901 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     6961 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     7021 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     7081 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     7141 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     7201 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     7261 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     7321 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     7381 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     7441 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     7501 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     7561 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     7621 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     7681 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     7741 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     7801 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     7861 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     7921 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     7981 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     8041 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     8101 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     8161 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     8221 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     8281 caaaaaacg
//
LOCUS       DAFFFQ010000102         7598 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000102
VERSION     DAFFFQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.104"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(942..1355)
                     /locus_tag="LQB69_002620"
     CDS             complement(942..1355)
                     /locus_tag="LQB69_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL3526739.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="LQB69_002621"
     CDS             complement(1811..2296)
                     /locus_tag="LQB69_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526740.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2680)
                     /locus_tag="LQB69_002622"
     CDS             complement(2435..2680)
                     /locus_tag="LQB69_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL3526741.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="LQB69_002623"
     CDS             2873..3673
                     /locus_tag="LQB69_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL3526742.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="LQB69_002624"
     CDS             3692..5560
                     /locus_tag="LQB69_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3526743.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="LQB69_002625"
     CDS             5553..6044
                     /locus_tag="LQB69_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL3526744.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="LQB69_002626"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="LQB69_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL3526745.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="LQB69_002627"
     CDS             6604..>7598
                     /locus_tag="LQB69_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL3526746.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2235 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca catgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg ttcccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DAFFFQ010000103         7401 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000103
VERSION     DAFFFQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.106"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(30..731)
                     /locus_tag="LQB69_002628"
     CDS             complement(30..731)
                     /locus_tag="LQB69_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL3526747.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(747..1187)
                     /locus_tag="LQB69_002629"
     CDS             complement(747..1187)
                     /locus_tag="LQB69_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL3526748.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1187..1762)
                     /locus_tag="LQB69_002630"
     CDS             complement(1187..1762)
                     /locus_tag="LQB69_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL3526749.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(2610..3089)
                     /locus_tag="LQB69_002631"
                     /pseudo
     CDS             complement(2610..3089)
                     /locus_tag="LQB69_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(3248..3949)
                     /locus_tag="LQB69_002632"
     CDS             complement(3248..3949)
                     /locus_tag="LQB69_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3526750.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(4057..5466)
                     /locus_tag="LQB69_002633"
     CDS             complement(4057..5466)
                     /locus_tag="LQB69_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL3526751.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(5514..7379)
                     /locus_tag="LQB69_002634"
     CDS             complement(5514..7379)
                     /locus_tag="LQB69_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526752.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
BASE COUNT         2110 a         1648 c         1229 g         2414 t
ORIGIN      
        1 tttctttccc gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg
       61 ttatccgtaa aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc
      121 tgacggatca ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc
      181 acttgtattt taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac
      241 gtttcataat aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt
      301 tttttgatac gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc
      361 cgtttttttg gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac
      421 tgctgtactt cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta
      481 tcttcataga tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct
      541 aaaaatcgct tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa
      601 taaaagagcg gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc
      661 tcttttgcaa gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa
      721 atcactgaca tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc
      781 aatgtttcaa aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt
      841 tgtgtaatca tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc
      901 aacatagttg ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag
      961 tcaaggatag caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact
     1021 tcctgcgccc atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag
     1081 tagaaacttg tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg
     1141 atttgctcct ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct
     1201 tcaataataa ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat
     1261 tgttggaaaa gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag
     1321 tttctaataa ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct
     1381 tgtcgggcgt cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac
     1441 ctagacctcc agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt
     1501 catcgatcgt ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa
     1561 aaagcccatt cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa
     1621 tcactcccat aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca
     1681 tgtattttcc taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac
     1741 cgcaaaaaac agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc
     1801 tacacgtgtt tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat
     1861 agacgccact atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa
     1921 caaaaataaa taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga
     1981 tttcgtccta tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat
     2041 taacgaaaaa atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa
     2101 taaaaaagcc gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct
     2161 aaaaatcttt ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac
     2221 tgctggaaga aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga
     2281 tattgggctc gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat
     2341 aataactttc tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga
     2401 cataaggtat accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt
     2461 tcaaggaggg tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat
     2521 tcgattcttt ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta
     2581 cttttttctt gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg
     2641 tcttcttcct ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat
     2701 aataaaatgg ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat
     2761 ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc
     2821 caaaacatgc ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt
     2881 gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga
     2941 aaagcggtct tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc
     3001 cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca
     3061 gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg
     3121 gtaatccaga aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga
     3181 agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     3241 tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct
     3301 ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga
     3361 tctgtgcgtc tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat
     3421 catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt
     3481 tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa
     3541 tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa
     3601 aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc
     3661 ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat
     3721 tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat
     3781 ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt
     3841 ctaattcact ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt
     3901 ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct
     3961 ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat
     4021 gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc
     4081 aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc
     4141 ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc
     4201 actgacaaga tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac
     4261 tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat
     4321 cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg
     4381 tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg
     4441 ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc
     4501 tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc
     4561 gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt
     4621 ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg
     4681 tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg
     4741 attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg
     4801 gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt
     4861 atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg
     4921 acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac
     4981 tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt
     5041 tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat
     5101 cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc
     5161 taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat
     5221 cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg
     5281 atcgtaatga tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca
     5341 gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc
     5401 aacggatagt ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg
     5461 ctccacccca taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt
     5521 acaattgcca gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg
     5581 gcaaatgcat cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta
     5641 attgcttcga tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg
     5701 actaagatct caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa
     5761 atctccgctc cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg
     5821 atctctccgt ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt
     5881 cccatcacgc cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc
     5941 gttaaaggac tttttaaggt atgccgtgct gaagtaatcg gttctcctgc attcgcatgt
     6001 ttgttttcta attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg
     6061 aatgcaagaa caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca
     6121 ccatccaccg tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa
     6181 gcttttactc cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca
     6241 atgaatggtt tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat
     6301 aacgaagtca ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat
     6361 gctacagcta aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat
     6421 acatcaaacc catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg
     6481 ccgaacatca cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta
     6541 aatccataaa tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt
     6601 cctaaagcaa gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc
     6661 atttgcagaa attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat
     6721 acggctagaa tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg
     6781 atcccaaaga aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca
     6841 agtgccatag caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta
     6901 aaagcaagca tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga
     6961 taagtaccgc tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc
     7021 aataccccgg cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg
     7081 atcagacgat tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct
     7141 tcctcatcca cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac
     7201 tgaccgccac tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct
     7261 gctaaagatt catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg
     7321 acattctttt gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt
     7381 gttttttcct tctttctttt t
//
LOCUS       DAFFFQ010000104         7400 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.107, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000104
VERSION     DAFFFQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.107"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            68..151
                     /locus_tag="LQB69_002635"
     CDS             68..151
                     /locus_tag="LQB69_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL3526753.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            276..1013
                     /gene="erm(B)"
                     /locus_tag="LQB69_002636"
     CDS             276..1013
                     /gene="erm(B)"
                     /locus_tag="LQB69_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL3526754.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1183..1443
                     /locus_tag="LQB69_002637"
                     /pseudo
     CDS             1183..1443
                     /locus_tag="LQB69_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1606..2271
                     /locus_tag="LQB69_002638"
                     /pseudo
     CDS             1606..2271
                     /locus_tag="LQB69_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2307..2552
                     /locus_tag="LQB69_002639"
     CDS             2307..2552
                     /locus_tag="LQB69_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3526755.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2655..3524
                     /locus_tag="LQB69_002640"
     CDS             2655..3524
                     /locus_tag="LQB69_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL3526756.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3505..4239
                     /locus_tag="LQB69_002641"
     CDS             3505..4239
                     /locus_tag="LQB69_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3526757.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4272..5184
                     /gene="ant(6)"
                     /locus_tag="LQB69_002642"
                     /pseudo
     CDS             4272..5184
                     /gene="ant(6)"
                     /locus_tag="LQB69_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5181..5723
                     /gene="sat4"
                     /locus_tag="LQB69_002643"
     CDS             5181..5723
                     /gene="sat4"
                     /locus_tag="LQB69_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3526758.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5816..6610
                     /locus_tag="LQB69_002644"
                     /note="aph(3')-IIIa"
     CDS             5816..6610
                     /locus_tag="LQB69_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL3526759.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6886..>7400
                     /locus_tag="LQB69_002645"
     CDS             6886..>7400
                     /locus_tag="LQB69_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3526760.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1556 g         2132 t
ORIGIN      
        1 tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
       61 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
      121 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      181 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      241 ttatttacag atgtaataac ttcaggagtg attacatgaa caaaaatata aaatattctc
      301 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      361 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      421 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      481 aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat
      541 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      601 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      661 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      721 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      781 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      841 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
      901 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
      961 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1021 agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta
     1081 gatatactac tgacagcttc caagaagcta aagaggtccc tagcgcctac ggggaatttg
     1141 tatcgataag gggtacaaat tcccactaag cgctcgggac cccttgtagg aaaatgtcct
     1201 aagtgtggca acaatattgt attaaaaaaa tcgttttatg gttgttcaaa ttatcctgaa
     1261 tgtaagttta ctttagctga acattttaga aagaaaaaac taaccaaaac gaatgtaaaa
     1321 gaattactgg agggaaaaga aaccctggta aaaggaatca aaaacaaaga gaaaaagccc
     1381 tacaatgccg ttgtaaaaat tggggaaaag ggatatattg attttatctc tttttcaaaa
     1441 taaacatggg gctgacccaa aagtagctcc tatatgaaaa atagaggtct ttccatttag
     1501 atagtagtgg aaagacctct atttatcttt tgaaagtgaa tacaaaaata aagcacactc
     1561 tgctataata taggtgatta atccatttga gagaggtgct ttatcatgta tcaaaattat
     1621 accataaacc aactttgttt gcctatagat cttgaaatta aattagaaga aaatgatttt
     1681 gctcatgcca ttgtgcagtt tgttgactct attcccgatg aagtctttct tccttattat
     1741 caatctatgg gtagacccca atatcatcct cgtatgctac tttccattat tctttgtgcc
     1801 tatattcaag ggggttattc gggccgccaa atacaaaata tgctgataga tagtattcgg
     1861 atgcgctact tatctcaaga gcaattccct aactttcgta ctattaatcg ttttcgcgtt
     1921 catccgatta tgaacgatat tctagatcat tcatttgttc aatttcgtga gctactggtt
     1981 caaagtggct taatcagtgg cagtgctcta tacattgatg gtacaaaaat tgaagcggat
     2041 gcgaataaat actctttcgt ttggaaaaaa agtattttaa agtataagga tagccttgat
     2101 caaaaagccc ttgaaaacta ccaacaaatg gtggaaacaa aaatcttacc tgagttaata
     2161 gatgatttaa aggatgaact ttctatagaa gagattgaaa ggattcgtca atctttggaa
     2221 gaaaaggaaa atcaattaat tgaagcgatt gaccaaaccg aaacagtaga agggggctga
     2281 cccattatta ataaggagtg gacaacatga ttgatgtaaa tgagctattg gaagaagcta
     2341 ttagagaaac tgaaaatcta aatgatggcg aagtttttct tgttaaagat ttattcaagg
     2401 gatatgtggg gaataggata cccagaaagg atcgacttct gcttggaacg ttatttttaa
     2461 accatgttag caaaatgaat ggtaatttaa aggctattga aaagacttca tctaatcagc
     2521 agaggtataa aaagacaatt gataagtttt aactatcttt tgtgaaatta aaaaaattat
     2581 aaatatgttg agtagaagca aaagatttgg cttggaattt ttacagcagt gaatcgataa
     2641 aaggagcatg cttgatggta gataatatta ttaaatcagt agcagagaaa ttatcctctc
     2701 tgtcttatat agaaggtatt gttttaggtg gttcacgtgc aaggggcacc catacagagg
     2761 attcggatat agatatcggc atctattaca attcagaatc atttgacata aatactatta
     2821 atcaattcgc tacaaagctg gatgatgagc atagaaataa ccttgttgta cctcccggag
     2881 catggggtga ttggattaat ggcggcggat ggttagtcat aaacgggtat catgtggatt
     2941 taattttacg tgatattaaa cgtgtggaac aaataatgaa agatacagag cacggaattg
     3001 ttactgccaa ttatcagact gggcatcccc atggttatat tagtgcaatg tatcgaggag
     3061 aattagcgat tagcaaaata ctatatgcta agaatgaaag cttatgcgaa ttaaaaaaac
     3121 aggcagaaac ttatcccaat gctttgcaga aaagtttagt taactttttt atgtttgaag
     3181 cagggttctc tttaatgttt gtaaaagcaa attcgggaac agacgataaa tattatattg
     3241 cgggtcatgt ttttcgtata gtttcatgtt taaatcaagt gttatttgca tgtaataatg
     3301 cttattgtat caacgaaaag aaagctataa aactgcttga aacttttgaa cataaacctg
     3361 aaaaatatac cgagaaggta aatcatattt ttgaagtact cggtatctca ctttttgaat
     3421 gctacgacat gaccgagaag ctttataatg aagtgaatga aattgtatcg gagataaata
     3481 actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa tatatttttt
     3541 caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg agaatgggag
     3601 actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt aggatgcggc
     3661 tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt aggtgttgat
     3721 atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca gattgaatat
     3781 gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt aatactaagt
     3841 tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat atataggatg
     3901 ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac tgctcatgga
     3961 acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt ggacaattat
     4021 tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa atatcataga
     4081 acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa tcagattgtg
     4141 gagccacagc cgccagagaa catgatggat attccgggga tggcggatga aatgcgacgc
     4201 ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa aaataaacga
     4261 ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct ttagcagaac
     4321 aggatgaacg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata
     4381 cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt
     4441 atatctaatg atgactggct taatcaattt gggaatataa taatgatgca aaagccggag
     4501 gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat
     4561 tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc
     4621 gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg
     4681 actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat
     4741 gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga aattttattt
     4801 gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat atcatggaag
     4861 gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt tattgaaagg
     4921 tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga ttcctatgaa
     4981 aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc cggtgaggtg
     5041 gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa atataccagg
     5101 gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata tgccgctgat
     5161 atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg aaagatatcg
     5221 ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat gaaaacgaga
     5281 attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac caagacgaag
     5341 aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact gataagataa
     5401 tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga
     5461 accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag
     5521 gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa
     5581 tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca
     5641 aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg
     5701 ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat
     5761 aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc
     5821 taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga
     5881 tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata
     5941 tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga
     6001 catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca
     6061 tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta
     6121 tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt
     6181 tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga
     6241 attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga
     6301 cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga
     6361 ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa
     6421 agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc
     6481 cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt
     6541 tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga
     6601 attgttttag tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat
     6661 ctaatctttt ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta
     6721 aaagttcgct agatagagtt ctatattaga gatgtaaaga gttttggtga agaggtttgt
     6781 gagtatggct tttataacca gggtgcaatg agaagcacta acagcagcta gcttaagaac
     6841 gcataggata tactttttat ggaagtcaca gaggaggaat aaaatttgaa caggattaac
     6901 agagtaacct ctattcttat tcagctgcaa tcaaaaaaaa taattcctgc caaagaaatt
     6961 gcacagcgat ttaatataag cttaaggaca gtttacagag atatccggac acttgaagaa
     7021 gccggaatac caattggatc tgaggccgga aaaggatatt ttcttgtaga gggcttccta
     7081 cttccgccgg taatgtttac agcggcggaa gtgggtgcat tgattacagc agggaaattt
     7141 ttaaattgcc atggagatga atcatttata aaggattttg attcagctat gtataaaatc
     7201 aaatctattt tgaagcatgg tgaaaaaaac tatgcacagg agttggagaa cagtattaac
     7261 gtgtacagca catctggaca gaaaaataca ttggcggata acgttattgc agcaattcag
     7321 actgcaatct gcaataaaag ggtaatatca attcaatatc ctgcatcagg agggcaagaa
     7381 ccggaaagca gaatgataga
//
LOCUS       DAFFFQ010000105         7342 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000105
VERSION     DAFFFQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.108"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            99..746
                     /locus_tag="LQB69_002646"
     CDS             99..746
                     /locus_tag="LQB69_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL3526761.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="LQB69_002647"
     CDS             1139..3784
                     /locus_tag="LQB69_002647"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL3526762.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="LQB69_002648"
     CDS             4094..5407
                     /locus_tag="LQB69_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL3526763.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="LQB69_002649"
     CDS             5397..6050
                     /locus_tag="LQB69_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3526764.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6776
                     /gene="radC"
                     /locus_tag="LQB69_002650"
     CDS             6105..6776
                     /gene="radC"
                     /locus_tag="LQB69_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL3526765.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6671..>7342)
                     /locus_tag="LQB69_002651"
     CDS             complement(6671..>7342)
                     /locus_tag="LQB69_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL3526766.1"
                     /translation="NKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDC
                     QGSVAGQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEA
                     TKQKFEQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALM
                     KELNEIEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNIT
                     VNFLI"
BASE COUNT         2385 a         1237 c         1534 g         2186 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttccggaata tataagttag
     6661 tacacataaa ttatataagg aaatttactg ttatattgcc atctggaaat actattatgt
     6721 tatcgataat tgaacgccat aatgcatttt tttcttcttc cgaagattct ttataaattt
     6781 catcgaattc ttttgataga ataaattcga cattttttat atctatttta gctggctcta
     6841 cttcaatttc atttaattct ttcattaaag cttctcgatc ttttttgtat tcctctaagt
     6901 ctataacttc attcaagtag gctttcttca atctatctaa tttattctca atatttttct
     6961 tactattttt ctttttaata atctttggtt gtttttttga atagtttatt tgtatttgtt
     7021 caaatttttg tttagtagct tcaaaaatat aacgttctaa atttttttcg taaaaagcgc
     7081 gcttatttgt gcaacgtaat ttattatttc ttcgttttac acatctataa caagtccttt
     7141 cgtatataga ttctgtaccg tcttttttct tatatcttgc tttaatagtt tgaccagcta
     7201 ctgatccctg acagtcagaa cattttaata aacctttaaa aatgtaatca cgttttgcat
     7261 ttgtttttaa atttttaggt aaggcttttt gaacagcata aaaaagtttt ttatcaataa
     7321 taggaggaca gtaattttta tt
//
LOCUS       DAFFFQ010000106         7318 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000106
VERSION     DAFFFQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.109"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(153..1238)
                     /locus_tag="LQB69_002652"
     CDS             complement(153..1238)
                     /locus_tag="LQB69_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3526767.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(1558..1965)
                     /locus_tag="LQB69_002653"
     CDS             complement(1558..1965)
                     /locus_tag="LQB69_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526768.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(1984..2802)
                     /locus_tag="LQB69_002654"
     CDS             complement(1984..2802)
                     /locus_tag="LQB69_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3526769.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
     gene            complement(3028..3321)
                     /locus_tag="LQB69_002655"
     CDS             complement(3028..3321)
                     /locus_tag="LQB69_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL3526770.1"
                     /translation="MSINTFVYSHPINVYIIKYLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSMNMTDVYEKLLILENEYDQFTSNQQRKVKKQID"
     gene            complement(3457..3636)
                     /locus_tag="LQB69_002656"
     CDS             complement(3457..3636)
                     /locus_tag="LQB69_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526771.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLFI"
     gene            complement(3633..3983)
                     /locus_tag="LQB69_002657"
     CDS             complement(3633..3983)
                     /locus_tag="LQB69_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526772.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRANQNLAKEQMRTE
                     EIAEILNASVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3976..5301)
                     /locus_tag="LQB69_002658"
     CDS             complement(3976..5301)
                     /locus_tag="LQB69_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3526773.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYQNVPDKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIKDLANWNPYTIKSRLGVIGLQLYFHANGIDRTDIAIAPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHHCKTGCVHLHIGTSILETRPGFSHQMKI
                     SITDNTRELQDYCLFLFNKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDDEK
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(5410..5613)
                     /locus_tag="LQB69_002659"
     CDS             complement(5410..5613)
                     /locus_tag="LQB69_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL3526774.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            complement(5773..6348)
                     /locus_tag="LQB69_002660"
     CDS             complement(5773..6348)
                     /locus_tag="LQB69_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526775.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            complement(6364..6969)
                     /locus_tag="LQB69_002661"
     CDS             complement(6364..6969)
                     /locus_tag="LQB69_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBL3526776.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         2132 a         1347 c         1077 g         2762 t
ORIGIN      
        1 ttaaaaccta cgtttgtgat tgctttcata aacttttaat ctaaaaactc cggaataata
       61 actatttatt tgtaacattt gtaataaaat taaaattata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcaataaacc agttttttac atacacaaaa atcaaattct gcaaattatt tactttctca
      301 gtcttttgtt tttgataagc tttcagcaac gtcatatata atcctttatc cgcatcaatt
      361 ccgtaagatt caagttcttc atacacaact accgtcttct caagttcctc ggctttatgt
      421 tttgcgttgt gtattgtggt gacggtttgt tgggcttctt cgtaagtgtt agaaaatgtt
      481 tttatcaagc ttaatacctt ttctggtaca aatgtgttta tgctgtcttc tttcatgatt
      541 tcaacgaagt tatctagcaa aacttgattt tgtaattggt ctgtttcagt gtcttttaag
      601 tgtcttttag tgtctaaaga tttattaata taataatctt gtgcgatttc cgcatttcca
      661 gtgttatcaa gggtttcagc tgttttttct cgacgtgcga tttccgcatt tccagtatta
      721 tcaagggttt tagcagtttc ctctcgacgt gcgatttccg cattccgaga agaggttgat
      781 ttatcaatag tttggcttaa atcgtcatcg aaagaattct gaataaaaag atcatcggca
      841 cttatttcag gttgaagtaa atagtattta tttttttcat tttttttcgt ttttgggtta
      901 aattctcctt ttacgactga aatcaaatta tatgtttcta actcgtttaa aatggttttt
      961 actttgcctt ggtagcaatt taatttgtcc attaggtctg cttgtgtaaa ttccaaatat
     1021 aatagaccat catcgtcgat ccatttttca tgtaaggctc tacgaaattc atttttaaaa
     1081 agcatatagg ctatttttga gctgtctttc attttagagt atttagaatt ctgaattaaa
     1141 aaactaggaa atttataata tttttcaata taaacattcg ttgctttata tccccaattt
     1201 ttaataatca ttttttgaga ttgaatttct gtagacatta aatttctcct tcttcataga
     1261 aacttgtata tattcacgat tttcaacaag tgaactatac catgtattaa gggtgaataa
     1321 aacactttca gtatttaagg tataagttat atattaaaaa aataagtata agttatactt
     1381 taaatacaaa taatgtataa cttatacttt aaataataat tttataatgt tgatttaatc
     1441 gcaaatttac acgttgttta ttaagttata ctttaaatat ttaatgtata acttatactt
     1501 taatattcat ttatctagta taacttatac tttaattgaa taccgcatga aaaaaaatta
     1561 ttttggtaat ttttcaaact cttcttgtat ttcttctctt atgtgttctt cttttgctac
     1621 tcctagtcta tgctggatat atgagtctgc taatgtatta acaatttttt cgaaagaaat
     1681 tctccctttt tcactttgta actccgtcat atagtcttgt aaaattgaga gtttgctatt
     1741 taatgccgga gagatctttt taggaactga taccttgttt cttttatcag gaattgaaac
     1801 tggtcggcct ctttttttca atgtaatatt ttttgtgtta ctcaatgcac tattgctaaa
     1861 gttatattca ccgcttattt tcacatcttg gcgtttgtta ttgggtaata atggctcaaa
     1921 ttcgccattc tccatcattt taggaatatc aaaatcttta ggcattactt atgcaactcc
     1981 ttattattta tttaaaaact cttgtctagc atctagttct gccaaaatat ttataaatac
     2041 ttggtgtgct ttataatccc attgatcaaa atttccattt ttgtattttc ttaaatgtat
     2101 accaatttta gaataccttc tcaatctttt ttgataatga atgagatttt tggataccac
     2161 ggttccgtat ttttcaattg cttcttctag gatttcttca tcaaaatcat catttggttc
     2221 taccatacaa ggtacaatat ctataatttg caaatcaaga tcgtatctat cgaccatgaa
     2281 tttttgataa tttacatatt tgttgactcc ctctaaggat tcttcagctg tttgaaatga
     2341 aattatcgaa tagtctgaag ctgccatagc attatcggaa aattcgctaa tggttggtgg
     2401 tacatcaata aatatttctt gataattgtt ctttattgga agaagtaatt ttttcaagac
     2461 agtaacttgg tctaattcat tttcatgttt attagcaaaa gcggagaatg atcgaaaact
     2521 agtgtcacat gccagaaggt caagattttc atttacctta ataatactac ttgctaattc
     2581 tccatttgcg acaccatttg ttatagtggt tttgatatcg gttataccga aagttcttgc
     2641 tatgacttgt gttaagttcg cttgagggtc taaatcaata ataagcgttt ttctatttct
     2701 tatcattgca ttgtcataag caagcatgct gactatctta gttttaccta ctcctccttt
     2761 aaaattgcca actgtaaatg tagtcccttg aaaagtatcc atgtaaatcc tcccaatctt
     2821 taattgataa ttaaagtata acttatatat cggaaatggt caacattaaa agtataactt
     2881 aaataataat atctaaaaac gtttggatat agcggttttt aagaataaaa gtataagtta
     2941 taccctaaat atatatggta taacttatac tttaattatt taaggtataa cttatacctt
     3001 agatattgct tttcgaaaat agctattcta gtctatctgt ttctttactt ttcgctgctg
     3061 atttgaagtg aattgatcat attcattttc tagtattaat aacttttcgt atacatcggt
     3121 catattcata ctagaagcaa gacttagatc atatacaaat gaagcaggta atgattttat
     3181 tctacgttgt cgggttatcc agcttgcaac cgttccttgt gggtaggcat ataactcaca
     3241 aaattgttct acagtgattc ctaaatattt gataatgtaa acattgatcg ggtgcgagta
     3301 aacaaatgta ttgatggaca tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa
     3361 taatagttta acacacgcat tattctagta tctactccta tttgtaaaaa caaaaaagga
     3421 ttcatagaga taactctata gaatccgtaa aataccttat ataaataacg ggaaaatata
     3481 aaacctaaaa ttatgataac ctgctgcagc ttctacatag aaagagaaac gctcaacacc
     3541 aacctcattt atcaaacgtt ggccataccc ccaaatctct tctgatagct gatcaaaggt
     3601 tatatacttt agctcatatt gctggataat gttcattcta atcgactcca tttattatcc
     3661 tggtaaaatt cgatatgacg tatttcgtcg tagtgaattt tgtctttgcc aacgtagatt
     3721 cccagctcat cactacctaa gatctttcca acaatatcgt ctggatagta tccttcagcg
     3781 tttcgttctt ctttttgaat acatactgat ctatttttta tgactgaagc gttcaatatt
     3841 tccgcaatct cttcagtcct catttgttcc tttgctaaat tttgatttgc acgttgctca
     3901 ttatctgcat cgatctcttc agtatgatcc gataaataga aacctgccca tttcaatcgt
     3961 cctcgatcgt tatagtcaat catttttaag cccccctgcg ccgcctgcat ggccaccaac
     4021 caacgtgctt ctagtaatac tacgagcgcc ttctaacatc gaagaagcat gtacaattga
     4081 cgtaaaacca tacttctgcc taattttatc tattattttg tctagttttt cgtcatcaat
     4141 ctgtttttgt ggatcactaa acaaatccag ctgaagcgaa tcagtataaa ctagcttgct
     4201 gtaagtgatt ccgacatggc ggacttcttg tccctcatag tatttattaa ataagaacag
     4261 acaatagtct tgtagctctc ttgtattatc tgtgatagat atcttcatct ggtgtgaaaa
     4321 ccctggtctc gtttctaaaa tcgacgttcc aatgtgtaaa tgaacacaac ctgttttaca
     4381 gtggtgttgc ctaatacgga ttgctacttg ctcagccatt tcttttacga ccagttcgat
     4441 ttcatttctt ctagtataat ctcgtggtag cacttgtgaa ttgccatatg atttttcctt
     4501 tgtcggttct ggtgcgattg ctatgtcagt tcgatcaata ccatttgcat ggaagtacag
     4561 ctgtaaccca atcacaccta aacggctctt aatggtgtat ggattccaat tagctaaatc
     4621 tttgatactc atgatcccca tttggttgag cctttttttc attcgagaac caatacccca
     4681 aaagtctgtc atttcaggaa tattccatac tttatcagga acattttgat acgtccattc
     4741 ggcaataaat ccttcattat gcttcgcttc attgtctaag gctagtttag ccaaaagagg
     4801 attatcgcca acacctactg tagcaatcaa tcctagctct tctttaatac gttcttggat
     4861 catttgagcg agattcttcc ttcgctgact tcgtgttcct tcagtcgtaa aaagattcag
     4921 tgatcgggtc acttttaaga ttgattcatc gatcgagtaa atcagtagat cttcatcagc
     4981 cacatatctt ctgaaaatat tatttacctg catattacgc ttaatataaa ggttcatacg
     5041 tggtggaaca acatgtaatg ttttagggaa aggctgtggt agatcccgtg gtctgctgac
     5101 attagaaata ccatatttct tctttgcagc tggtgaagaa gctaaaatca accctgatcc
     5161 tgtattatcc gctcgactca tgacaactaa ttcagtcgtt aatggatcta aatttctttc
     5221 tatacattcg acacttgcgt aaaaactttt catatcgatt agaaagtagt cattgactgg
     5281 ctcttttgag taatcaagca taaagttctc cccctcttct ctcatattat acaaacatac
     5341 gttcgtgttt gcaacgagga tcttctctaa atagaaaaat agccagacaa ttgtctgact
     5401 ataataaacc tatttgtttt tttcgtttag caattggaag tcttgatcaa tgaattggta
     5461 acaattccca caatttttac aattgtattg tttcgtttga atattgagtt gaatcaactc
     5521 accacaagca cagtcaatcg atacagccgt tcttcgttct aatccgtata atggacgtcc
     5581 ccaaagagtg tagcctaatt gttgttcttt catttacttc gttcctccct aaaaatatgt
     5641 ctattcgtag ctggtatgac gcttcgttcg accaatgata catcttaatg agtgaacctg
     5701 accttagaat gctcgtataa acaacgattt atttgattta aaagagattt ttatattaat
     5761 tttatcaatc ttctactcac tcgcttctat ttgtttctta attctaaaaa gtgtactacg
     5821 actgaaaccc gttatggctt ccacttcttt atacgatttg ccggaagtaa tcaattcata
     5881 tgcatgcctc ttctttggtg tgattgttgc tttcggacgt ccctctttaa aattaggatt
     5941 attctttttc gcaaataatt tcccttcctg agtacgttct acaatcatat cacgttccat
     6001 ttcagctaca ctcaacaacg tacgtgtaac cattcgaccc atcgatgtat tttcgatcgt
     6061 ccctagattc aataccttaa tcgtgacgtt atcatcgagt aaaggttcaa tgatctctag
     6121 tgcttctctt gtattacggg cgaatcgatc aagtttcgta acaacgattt cgtctccagg
     6181 cttcagacca gccaacagct gattaaactt tggccgatct cgtttcgtac ctgtaaattt
     6241 ttcagaaaaa atattcttct tctcaacacc gttctcaagt agctgttgta tctgttcctt
     6301 tgtactttga gaagcggttg agacacgtgc atagccatat tttgtcattt aactcacctg
     6361 cttttacaaa tcatctatcg tatactcaga gtatccttca tagaagtagc tgacatcgat
     6421 tcctttcata tataactcac gatcgtcaat tttatcagtc agggcattgg aaatgagata
     6481 gcgtatttcc aagtcattta ttggactgcg ctccattgca gaaagatagt ctgctttatc
     6541 gatcaagttc caatcgacca ctttttgcaa ttcttccttt aaaatgagat ccagccaaat
     6601 tctagtactg cgcccgtttc cttcgcgaaa agggtgagcg atattcattt ccacgtattt
     6661 cttaataatc tcttcgaaag aagtttgcgg catctgatca atatgattta aagattgttc
     6721 taggtacatg accggcgcaa aacgaaaatt tccttttgca atattaactg tccgaatttt
     6781 tcctgcaaaa tcataaattt ctgaaaagag aaaatcatga atttcagaca agcctttaaa
     6841 cgtgccgact tcgatttcat ttatttttcc agaatcatat aattcttttg ctttttgctt
     6901 actgagtttt tcttcagcct tggccaactc aacctgattc gttaaaccca atttattttc
     6961 taaaatcatg gaaaatcccc ttaacttttt gacataagtt tatgaaacac tacaaatcct
     7021 attgtatcgc gaatttaacc atgtgtcaaa aactattaag tttttgacac gtagttagtt
     7081 ttcaatagaa gccatctgtc cttttctttt taaatggcaa aaatttttta ttttattcgg
     7141 cacaaactat aaacatttaa tcgctaagaa aatcactcaa ttattttgag tgattttttc
     7201 agttcttatt ttaaaacaaa gtgttcataa aatcaggtgt ttctttattt ttcagtagca
     7261 taccctgttt ttattttgag tattagtaat aattaaaaaa accacttgta attttata
//
LOCUS       DAFFFQ010000107         6962 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000107
VERSION     DAFFFQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.110"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..193
                     /locus_tag="LQB69_002662"
     CDS             <1..193
                     /locus_tag="LQB69_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526777.1"
                     /translation="GRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPL
                     VPKGYDFLDSISKLEKMND"
     gene            271..945
                     /locus_tag="LQB69_002663"
     CDS             271..945
                     /locus_tag="LQB69_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL3526778.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            938..1102
                     /locus_tag="LQB69_002664"
     CDS             938..1102
                     /locus_tag="LQB69_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526779.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            1178..2320
                     /gene="dcm"
                     /locus_tag="LQB69_002665"
                     /pseudo
     CDS             1178..2320
                     /gene="dcm"
                     /locus_tag="LQB69_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            2310..2600
                     /locus_tag="LQB69_002666"
     CDS             2310..2600
                     /locus_tag="LQB69_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526780.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2859..4043
                     /locus_tag="LQB69_002667"
     CDS             2859..4043
                     /locus_tag="LQB69_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL3526781.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4040..4177
                     /locus_tag="LQB69_002668"
     CDS             4040..4177
                     /locus_tag="LQB69_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL3526782.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            4923..6833
                     /gene="ltrA"
                     /locus_tag="LQB69_002669"
     CDS             4923..6833
                     /gene="ltrA"
                     /locus_tag="LQB69_002669"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL3526783.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         2437 a         1118 c         1539 g         1868 t
ORIGIN      
        1 gggtcgtatg agtgaactcg gatattccat gatgtttggg gaggtcgaaa aaaacttctt
       61 tatgaagcac atcaaaggtc gaggttatgt ggatacaggt ggcagtgtga ttagtgagtt
      121 ttatacgcca cttgtaccaa aagggtatga tttcttagat tcaattagta aattagaaaa
      181 gatgaatgat tgagtgatct tcaatactga ctttatactt caagtaacgt ataatacttt
      241 tatatagtag gaagaaaggg gataattaat atggaaagta atgattctta tttagagtat
      301 gcaaaagcaa ataaggataa gtttatttct tatattatca aagggaaatc ccccgaagtt
      361 ggacgagacg cagtttttat ggcagggagt cctggggctg gaaaatcaga ggttgccatt
      421 ggattagctg agaattatga taaccatgtg attattgatg cggattactt tcgcacccag
      481 tttcctgatt ataatggaaa aaactctagt ctctttcaaa aagcatcttc ttggttggta
      541 gagcaggcat ttagatatgt cttagatcat ggctattctt ttattctgga tgcaactttt
      601 gcaatattaa gtgctgaaaa aaatatctcg cgggcgttga aaaaacattt tcgagtgacg
      661 attttctatg tttatcaaga ccctaaaatc gcttggaaat ttacacaaga acgtgaaatt
      721 gcagagggac gtcatgtgcc gaaaaaggca tttattaacg cttttttcaa atcaagagag
      781 aacattgcaa aagtaaagag taaacatcca gatgtattgc ttcatatcat aataaaagat
      841 tatacgaata atatttccga agttcattat gctacggata atctagaatt atttttacca
      901 ctagggtata gtaggaaaga tttggaggtt caattacatg actgagaaag aacaagtaac
      961 gaaaattgta aagaaataca ataagagcat tgcggattta tcggaaaatg ctactgcaaa
     1021 agagttcaaa acggtgatta aatatgtagc cgatcaggcc aatgaaaaac aacggaaatt
     1081 agttggatta aataaaaaat agtaggacaa attaataatg tatgagccaa gagcaaaatg
     1141 ttcttggctt ttttctttgc aatgaatgag gtaatgtatg agatttttag atttattcgc
     1201 cggtattggt ggctttcgtt taggtatgga acaggcgagc catcattgta tcggtttttg
     1261 tgaaattgat aaatttgcta gacgtagtta taaagcaatt catgatacaa gtaaggaggt
     1321 ggaaatgcat gacatcacaa gtgtatcaga tgagtttatt caatccctcg gaccagtgga
     1381 catcctttgc ggcggatttc cgtgccaagc tttttcaatt gcgggaaagc ggcaaagatt
     1441 ttccgatact cgaggtactt tattctttga aatcgctcgg ttcaccactc ttctccgacc
     1501 taagctttta ttccttgaga acgtccgagg attgctcaat cacgaaggag gggctacgtt
     1561 cgagacgatc ctccgaacgc tggatggatt ggggtatgac gtggagtggt aggtgcttaa
     1621 ctcaaaggcc tacattcccc aaagccggga gcggatattc cttatcggac attctcgaga
     1681 cgcatgtacc gaacaagtat ttcctatcat tggaccgtct tcaacatctg atcaaaacat
     1741 cagaaatttg gtaaatatca atccttccaa tcgaggaatg ggtggacaag tctatggttc
     1801 agatggcgta gcgccaacct taacaggtga tgaaggaatc aaaatcgcac tgccagcaag
     1861 ggatggaatc tctgtagcag gactattacc gggaaagttt gaacaaggta atcgagttta
     1921 tgaagttact ggaaccgccc ccaccttgtc aacgaaacaa ggtggtacaa aaatcatgat
     1981 tcgaaaaaat gaaacagaat atcaagaggt gaagccagaa gacagtgtga atctggcttt
     2041 tcctaattcc acaagtcgcc gaggtcgtct gggaaaacag agtgttcata ccttactaac
     2101 aggagaccaa caagcgatcg tcacggatca gtatcaaatt cggaagctaa cacctagaga
     2161 gtgttggcga cttcagggct ttcccgattg ggcttttgat cgagattctc aagtcaattc
     2221 agatagtcag ttgtataagc aagccggaaa ctctgtaaca gttccggtca tttttgatat
     2281 tgccagaaga ttaaaagagt tggatgtaca tggtttatga tgagttgatt ggagaaattt
     2341 attgggtgat tggaaagatt cagtcagacc cagaattaga agaacaattg cgacggttaa
     2401 aatttgatat tcgcaagaat ggtgtcaaag tcgctggcga tccatattta atgaatgagg
     2461 agacggatgc tcgaattgaa ataaatcaag taattgcaga atttgagcgg attgcggacc
     2521 aagcaaaaga accagatatt cgtcaatact tgttagaaat gaaagcagag ttggaagtaa
     2581 atggtattac agatgagtaa cctaaaccaa cacaactgac tgttaggcgc tgaccgcgga
     2641 ggattcgcga agcagaaatc ctccgtggtg ctgtgcctgt tcatggacgg cgttagccga
     2701 ccatgatgtc tcacccccgt atctaacagg ggggtacaaa aacacaaaaa ataagtaagc
     2761 aaccattaag aatgcttgtt gtatcaaaga tagtaagtaa tttgtatagg tgttaactca
     2821 tttgaaaaag ttaacacctt gaatttggag gtgagaactt ggcacaacgg aatttagatt
     2881 atcggctatt aaaagaccga cgcaacgaat acggcgtttc acaaaataaa ttggctacgg
     2941 cttgtggact tagtcggccg tacttaaatc agatagaaaa tggtggcgta accgcatcta
     3001 taaaaaccat gaggaaaatt tttgaccaac tggaaagctt taatcccgat ttgcctttga
     3061 cgttactgtt tgactatgta aggattcgct ttcccacaac ggatgcacga aaaattattc
     3121 aagaaattct ccatttgaaa tttgagtata tgctccatga agattatgcc ttttactcct
     3181 atcaagaaca gtatgtcatg ggtgatattg tggtgatgtt gtctcatgaa gaagataaag
     3241 gtgtgctttt agaattaaag gggcgaggtt gccgacaatt tgaaactttt ttactcgctc
     3301 aaaagcgtag ctggtacgac tttttcgagg attgtctaaa ggccggtggc gtgatgaaac
     3361 gcttggactt ggcaatcaat gatcgagtgg ggctattgga tatacctgag ctaacgaaaa
     3421 aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt taaaagttat cgttccgggg
     3481 aacttttaaa ggctgatgaa aaggacggca tgggcaatac cttatatatc ggcagtctca
     3541 agagtgaggt ttatttctgt ttgtatgaaa aagactatga acaatatatc aagttaggga
     3601 ttccattaga ccaaactgag acgaaaaatc gttttgagat tcgactgaaa aatgaccgtg
     3661 cttttcatgc gattcaagat ttgttaaaag gtcgtagcat tgagagcacc acgttttcca
     3721 ttatcaatcg ttacttgcga tttgcggata aagtagaagg taaaaggcga accaactggc
     3781 ccttgaatga acaatggggg cgttttattg gacggaatcg gaaagagatt caattaactt
     3841 ccgagccaaa accgtatacc attgaacgaa ccttgaattg gctaggccga caagtcgccc
     3901 ctacgtggaa aatggctaag gagttggatc gattgaatcg gacaacctat atccaggata
     3961 tggtacagaa tgcacgctta tcagaccgac ataaaaagat tttggagcaa caaagtatgg
     4021 caattgaaaa tttgattata tgaaaagagg aatcgtcatg ggaattgtaa aagatatttt
     4081 tttggtattt ggtggcagta tgcttggtgt tactgtgatg tgcttgatga atgcaagtgc
     4141 agcagcggat cgagcaatgg aaaagaaagg aaagtgacaa gatgaatttc ggacaaaatt
     4201 tatgtgcgac tcgttcctta gtgaaaagct aaggcactaa taaaagaacg aaaaagtttg
     4261 gttattagga gaactgataa tctgacaggt ggaatgatga agtaacgctc tgaaacgcct
     4321 gccctgagcc tccgacaagc attgtatgag gaaactcaga atgtttgaag ctcggtaaag
     4381 acggctgaaa gataccgtaa tagttgatga acatatatct tctatcgaaa actatccaga
     4441 ggtgggtagc gtatccaaca ggtcgggggt ctataaaata tctatggtta gaatgttcta
     4501 taaggaactg acgaaacagc gaatgtacgg gtctaaattt gaactttgta gaaatgcaaa
     4561 ggtacgagaa atcgtagctg gtgtggcaaa gtaagaatat aggttatgaa atgccatata
     4621 acgttacagg cggtatcaag ccagcaggct tatagctagt gacctaagga tatatatgta
     4681 tagataagat tatcggaacg aggaaaggta ttgggttctc atcaagagaa taaactgacg
     4741 aagaacaata agcagttgaa ctaatgctga aaagcagagg gcgaacctaa gaaattcttg
     4801 taatgagaat ggaggaatgc cctcaagtcg tgtatatatt agaaaagtat tatgttgttt
     4861 caataaggat tacgagtaag ataaaaaagc tcactcaatt tcaagtaaag gagtgaagcc
     4921 ttatgcctaa aaataaaaat gaaacattgt gtgtagaaga tttaagacac gctgaatatt
     4981 acgaaatgca aaacaccttt gatgatttat atgccaaaag taagaatgga gatattttca
     5041 ctcatcttat ggatattatc ctatcgagag aaaatatatt actggcatat cgaaatatca
     5101 aagcaaatgc aggcagtaaa acagcaggaa cagacggtac aataattaaa gatattggca
     5161 aattgccagc agaaacagtt gtcaaaaaag taagatacat tgttgcaggc agtccacatg
     5221 ggtatcgacc taagccagtg aggcgaaaag aaataccaaa accaaatggt aaaaccagac
     5281 ctctaggtat tccttgtatg tgggacaggc ttatacaaca atgtataaaa caagtattag
     5341 aaccaatctg cgaagctaaa tttagtgaaa acagctatgg ttttagacct aataggtcag
     5401 ttgaaaatgc aataaaagca acctataacc gcctacaaat cagccagcta cattatgtga
     5461 ttgaatttga tattaagggt ttctttgaca acgtgaacca ttccaagctg ataaaacaga
     5521 tatgggctat gggaatacgg gacaagcact tgatattcat tttaaaaagg atattaaaag
     5581 ctccaatcaa aatgactaac ggtacaataa catatccaga gaaaggaact ccgcaagggg
     5641 gaataatatc cccactacta gcaaacattg ttcttaatga gttagaccat tgggtagaaa
     5701 gccaatggca ggaaaatcct gttacaaaaa actatgtggt tcatatcaat aaaagcggta
     5761 gcccatgtaa aagcaacgct tacaaagaaa tgaagaaaac aaagttaaag gaaatgtata
     5821 tggttcgtta tgcagatgat tttagagttt tctgccgcta caaggaaagt gctgaaaaag
     5881 ccaaaatagc aatcactcaa tggattgaac aacgattaaa attggaagta tcacaagaaa
     5941 aaacaagaat tgtaaatgtt agaaaaagat actccgactt tcttggtttc aagattaaaa
     6001 tgataccgag aagaaagaag ttagttgtta aatcacatat aagtgataaa caattcaaaa
     6061 accaaaaaga caagctggta tcacaggcaa agaaaattgc ttctccctca aaagggaaaa
     6121 cggaattaga agaaacaaga ctttataatt caatggtatt aggtatgcaa aattattatc
     6181 agattgcaac gaatgtaaat ctggattgta tgaaactgaa tagagctgta atggctatct
     6241 tcactaacag attaggtaca aacaaaaggg gcagattgag aaaaacaggc agacaactta
     6301 cgacaaagga ggcaacaaga tatggtaaat ctaaaatgct tcggtatgtt gctggcgagg
     6361 gcgaacctat tttccctgtt ggatatatcc aacacagaaa accaatggca aaggtgtatt
     6421 tagcgaattg ctacactcta gagggaagaa aatttatcca caccaatcta agtgttgata
     6481 aattactgat gtaccagtta atgaaattat ctttggaaaa tcgcacaata gaatacgcag
     6541 acaatcgaat ttcgctattt tctgctcaac atggaaaatg tgctataact ctggaagaat
     6601 ttcaacaagc taatgaaata cattgtcatc atattgtacc aactggagct ggtggaaatg
     6661 acgattataa aaatcttatt ttggtaaaag aggcggtaca ccgtctaata cacgctaaaa
     6721 cagaagaaac aatacaaaaa tatatagctc tattgaagct aaataaagtc cagctcataa
     6781 gactaaataa atatagagta ttagctggca atcaagaatt aaacttgata taggtctact
     6841 acataaatac ttttcaatat aaacacgatg gaacgcggag tgcggtgaaa gtcgcacgct
     6901 ccgtgtgaat ggggggaaaa gtttgagata tttataatat caggaactta cctatccata
     6961 ta
//
LOCUS       DAFFFQ010000108         6644 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000108
VERSION     DAFFFQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.111"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(1..859)
                     /locus_tag="LQB69_002670"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="LQB69_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="LQB69_002671"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="LQB69_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL3526784.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYITSKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="LQB69_002672"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="LQB69_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="LQB69_002673"
     CDS             complement(2143..2373)
                     /locus_tag="LQB69_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBL3526785.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="LQB69_002674"
     CDS             complement(2603..3421)
                     /locus_tag="LQB69_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL3526786.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="LQB69_002675"
     CDS             3582..4271
                     /locus_tag="LQB69_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL3526787.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="LQB69_002676"
     CDS             4285..5787
                     /locus_tag="LQB69_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL3526788.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="LQB69_002677"
     CDS             5800..6276
                     /locus_tag="LQB69_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3526789.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="LQB69_002678"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="LQB69_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1033 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattac aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAFFFQ010000109         6458 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000109
VERSION     DAFFFQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.112"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(105..560)
                     /locus_tag="LQB69_002679"
     CDS             complement(105..560)
                     /locus_tag="LQB69_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526790.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(714..1922)
                     /locus_tag="LQB69_002680"
     CDS             complement(714..1922)
                     /locus_tag="LQB69_002680"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL3526791.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2034..2366)
                     /locus_tag="LQB69_002681"
     CDS             complement(2034..2366)
                     /locus_tag="LQB69_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL3526792.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2514..3389
                     /locus_tag="LQB69_002682"
     CDS             2514..3389
                     /locus_tag="LQB69_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL3526793.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3471..4052)
                     /locus_tag="LQB69_002683"
     CDS             complement(3471..4052)
                     /locus_tag="LQB69_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL3526794.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4058..5317)
                     /locus_tag="LQB69_002684"
     CDS             complement(4058..5317)
                     /locus_tag="LQB69_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL3526795.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5319..6365)
                     /locus_tag="LQB69_002685"
     CDS             complement(5319..6365)
                     /locus_tag="LQB69_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526796.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1796 a         1338 c         1102 g         2222 t
ORIGIN      
        1 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
       61 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
      121 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
      181 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
      241 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
      301 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
      361 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
      421 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
      481 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
      541 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
      601 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
      661 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
      721 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
      781 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
      841 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
      901 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
      961 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
     1021 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
     1081 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
     1141 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
     1201 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
     1261 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
     1321 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
     1381 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
     1441 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
     1501 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
     1561 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
     1621 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
     1681 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
     1741 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
     1801 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
     1861 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
     1921 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
     1981 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
     2041 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
     2101 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
     2161 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
     2221 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
     2281 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
     2341 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
     2401 aacgcaaatc ctagataaga gcacttgaga aaatcgaatt tccatgctac atttaggaag
     2461 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
     2521 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
     2581 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
     2641 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
     2701 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
     2761 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
     2821 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
     2881 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
     2941 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
     3001 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
     3061 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
     3121 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
     3181 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
     3241 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
     3301 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
     3361 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
     3421 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
     3481 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
     3541 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
     3601 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
     3661 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
     3721 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
     3781 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
     3841 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
     3901 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
     3961 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
     4021 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
     4081 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
     4141 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
     4201 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
     4261 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
     4321 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
     4381 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
     4441 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
     4501 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
     4561 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
     4621 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
     4681 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
     4741 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
     4801 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
     4861 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
     4921 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
     4981 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
     5041 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
     5101 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
     5161 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
     5221 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
     5281 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
     5341 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
     5401 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
     5461 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
     5521 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
     5581 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
     5641 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
     5701 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
     5761 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
     5821 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
     5881 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
     5941 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
     6001 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
     6061 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
     6121 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
     6181 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
     6241 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
     6301 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
     6361 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
     6421 agataagagc aaagctcacc tcataacttg gaaataaa
//
LOCUS       DAFFFQ010000110         6407 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000110
VERSION     DAFFFQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.113"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(1041..1706)
                     /locus_tag="LQB69_002686"
     CDS             complement(1041..1706)
                     /locus_tag="LQB69_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3526797.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1886..3868
                     /locus_tag="LQB69_002687"
     CDS             1886..3868
                     /locus_tag="LQB69_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL3526798.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3872..4510
                     /locus_tag="LQB69_002688"
     CDS             3872..4510
                     /locus_tag="LQB69_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526799.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4538..4966
                     /locus_tag="LQB69_002689"
     CDS             4538..4966
                     /locus_tag="LQB69_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526800.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5423..6334
                     /locus_tag="LQB69_002690"
     CDS             5423..6334
                     /locus_tag="LQB69_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3526801.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADNGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2217 a          974 c         1179 g         2037 t
ORIGIN      
        1 acatcaaggt tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag
       61 ccgagattat cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt
      121 ggtgttcgta gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt
      181 gataaggaag ataagataac tgtgttatat aaactaggta actaactgta acaagctaat
      241 tttaagagat tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt
      301 aattgtcatt tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga
      361 atcagttgga aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc
      421 cactaacaag agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc
      481 taccgaaatc ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca
      541 ttactcaaag tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg
      601 cgcttgaaca atccattttg tattttattg agtactcttg gataaactac cagcgattat
      661 catatttatt tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat
      721 ataaaaaaga actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat
      781 tacttactag attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg
      841 tccatccttg attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac
      901 tataagccag aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc
      961 tttttcaatt aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta
     1021 tctctacgtt catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg
     1081 tcgtcatagt cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc
     1141 aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt
     1201 tctacctctc cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc
     1261 caacgttcga tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt
     1321 ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa
     1381 agatagacca aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt
     1441 tgttttgatg aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt
     1501 gattcggtag ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa
     1561 agtgacagca tttcaaggtc tggcattgta cgagaatttt cccaactaga aatcgtttta
     1621 tcggaaacac caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa
     1681 gttcgtattt tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt
     1741 tattctacat gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt
     1801 tatcaaagaa tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt
     1861 actcacaatc atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca
     1921 ggtactctat cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt
     1981 gccacaacat tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt
     2041 ctcatcagaa actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga
     2101 tacatcttgg aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga
     2161 tgtcaaatct tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat
     2221 tgaagaatcg atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa
     2281 agaagcctat gaacggttca tttcactatt cactgatcga ttcaatcaag catttcacac
     2341 tgattatgcc actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc
     2401 aaatacagac gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct
     2461 tttgttatca tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga
     2521 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
     2581 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
     2641 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
     2701 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
     2761 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
     2821 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
     2881 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
     2941 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
     3001 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
     3061 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
     3121 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
     3181 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
     3241 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
     3301 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
     3361 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
     3421 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
     3481 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     3541 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     3601 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     3661 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     3721 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     3781 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     3841 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     3901 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     3961 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     4021 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     4081 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     4141 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     4201 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     4261 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     4321 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     4381 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     4441 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     4501 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     4561 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     4621 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     4681 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     4741 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     4801 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     4861 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     4921 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     4981 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     5041 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     5101 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     5161 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     5221 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     5281 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     5341 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     5401 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     5461 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     5521 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     5581 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     5641 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     5701 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     5761 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     5821 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     5881 tgatgcaaaa gatgtgaaga aagctgctga taatggagac gatttctcta aattagccaa
     5941 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     6001 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     6061 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     6121 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     6181 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     6241 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     6301 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     6361 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaa
//
LOCUS       DAFFFQ010000111         6286 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000111
VERSION     DAFFFQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.114"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..177
                     /locus_tag="LQB69_002691"
     CDS             <1..177
                     /locus_tag="LQB69_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526802.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="LQB69_002692"
     CDS             190..690
                     /locus_tag="LQB69_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL3526803.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="LQB69_002693"
     CDS             787..2634
                     /locus_tag="LQB69_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL3526804.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="LQB69_002694"
     CDS             2637..3533
                     /locus_tag="LQB69_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3526805.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="LQB69_002695"
     CDS             3583..3972
                     /locus_tag="LQB69_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3526806.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6286
                     /locus_tag="LQB69_002696"
     CDS             3959..>6286
                     /locus_tag="LQB69_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3526807.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1887 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagaggcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaac
//
LOCUS       DAFFFQ010000112         6255 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000112
VERSION     DAFFFQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.115"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            54..506
                     /locus_tag="LQB69_002697"
     CDS             54..506
                     /locus_tag="LQB69_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526808.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            520..1209
                     /locus_tag="LQB69_002698"
     CDS             520..1209
                     /locus_tag="LQB69_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526809.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1334..3685
                     /locus_tag="LQB69_002699"
     CDS             1334..3685
                     /locus_tag="LQB69_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL3526810.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3765..4160
                     /locus_tag="LQB69_002700"
     CDS             3765..4160
                     /locus_tag="LQB69_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526811.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4485..5102
                     /locus_tag="LQB69_002701"
     CDS             4485..5102
                     /locus_tag="LQB69_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526812.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5143..5442
                     /locus_tag="LQB69_002702"
     CDS             5143..5442
                     /locus_tag="LQB69_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL3526813.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5476..5643
                     /locus_tag="LQB69_002703"
     CDS             5476..5643
                     /locus_tag="LQB69_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3526814.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5688..6251)
                     /locus_tag="LQB69_002704"
     CDS             complement(5688..6251)
                     /locus_tag="LQB69_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526815.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2150 a          865 c         1252 g         1988 t
ORIGIN      
        1 tatcttatcg tagatatata ttataaattt aataagaaag cgaggtgaga atcgtgaaaa
       61 aaacgatact aagtattttt tcagtattta caatattaat tgggggctat tttcttttga
      121 atttagattc tgttaattca aacgctcaaa taaaagatca aagagtagct gaacataata
      181 ataaaaaaat gagtttgacc aaaaatatct attctatgtc agatgaagaa ttgtataaag
      241 aatttggtga tttatccggg gaacagttag aagaaagtat tccaggaatt agtgactcag
      301 ataaaattgt tgtcttgtct gctggtgggc aattaatggg agatggcaaa tccgaattag
      361 ttagttataa acaaaatcac agacagttta ttgatgttaa tactgattcc aacgctattt
      421 cgattaagga tctaaaagag cttttaaata gtaaaagaaa tgaattaaag cagataaaag
      481 aaactaattc agtggaatta gagtaaagga agattcatta tgaaaaagat ttttggtgtt
      541 tctgttgggc tacttatgat gttaacattt ggaaatgcag tatatgctgc tgagaaagaa
      601 gttatttcag atccagagca gtacgcctca cgatactttg ctgattttga agaagaagat
      661 aaagtacttg cattgccaga aggtggtttt ttaaaagggg aagctaaaat tgtatctatg
      721 gataatccag atataattca aggttcatat aattcagaaa ctgatcctgc ttcagtttca
      781 gtgacagaag caaaaaaaat tgttgctgaa aaattcaaaa atgatattga aactactcca
      841 attatgcctc gaggagcatt tccacccgat gttggtacta gcggacacct tcttgtacta
      901 gcaggaggag gaagctataa atcacagcca tttagtggtg cgggatggcg ttttggaggt
      961 atgggtgtaa aaccagctga tggtacaggc aactttttac gttggaaatc ctacattgac
     1021 gatggaagag taggaaatgt aaatcaagcc aacgcaacta aatctggttc tattcaagga
     1081 atggcattgt atgctcgtga aggattcaaa tactttgcac caaacgggcc tggttggaca
     1141 atgaatatag gtatttatta cacttataat cctgttgcag gtacgtatta tgttgttcaa
     1201 aatatttaat aaaacagcac aaagagattt ctttgtgctg ttttattatt tttagttgtt
     1261 gacaaacact aaaatataaa atataatcaa cacataaacg aacgtatgtt cggcaagaaa
     1321 gcaggggatt tttatggatg agatggtttt aagtactcag aaatggctaa acaagaaata
     1381 cagtaatgtt acagggtttg ataaggttcc agaaaatggg cgaacagggt ggcctacaat
     1441 ctatggactt attgaaggac tacaagtaga acttggtatt actaatctag tagctaattt
     1501 tggacctacc actgaaaaaa tgtacgataa tcaggttact cctaaatggg gcaaaaatct
     1561 acctaaaaat attgtatttc ttattcaagg tgccttttgg tgtaagggga ttaatccagg
     1621 tggcttcgat ggggtatata ctccatattt agatactgct gtaaaagagt tgcagactga
     1681 tgcagggttt aatggaagcg ctgtaactgg agttttggat gcgaaatggg ccaaagcgtt
     1741 atttgatatg agtgcttttg ttcttgttcc cggcggagat agtaaaatta gaaaaatgca
     1801 gcaatggtta aatgtaaatt atttagaata cacgggaata atgccttgtg atggtattta
     1861 tcaaagaaat tcaaatcaag cattaatttt tgcattacaa gctgaactag gatattctcc
     1921 cagtgaagca acagggtctt atggtaatgg gacaactagt aaaacttatc ctgtgtctga
     1981 aggtaactcg ggaaactatg taagaataat tcaatatgga ttatatgtta atggttattt
     2041 tgaagacggt acattcgatg gtattttcac taaatatatg ggtttagaag tactggcatt
     2101 ccgaaaattt atggtattgc ctgaatatac tgaaatagca gatgttgttg taataaaagg
     2161 actattgtct agcgctggag atattagaag atctgcagat ggcgcagata cttctacgca
     2221 attaactaga tctcaaatcc aaacattggt tgataatgat ataaaaatag ttggtagata
     2281 tcttactggt actgttggaa ttggaaaaga tgaacgaaat aagtatctaa ctacagagga
     2341 attgaataat attttttcta aaaatttatc tgtgtttcct atctatcaag atggaggtgc
     2401 agcattagct tattttactt atgatcgtgg cttatcagat ggtaagaagg ctatagatgc
     2461 agcaaaaaat ctaaatatac cattaacaac agttatttat tttgctgttg atctagacat
     2521 gttaggggaa gaaattcttg catatgctgg agaatatttt aagggggtat ctgctgtaat
     2581 gtcatatagc gggtatcaaa cgggtgttta cggaacaaga aatgtttgtt ctcaaataat
     2641 caataacggg tatgcttcgt ttgcatttgt ttcagatatg tcgactggtt atagtgggaa
     2701 tttaggtttt cctatgccta gacaatggtc ttttgatcaa tttgttgaat ttaccatagg
     2761 aagtggcaac ggagctgttg gtatcgattt aatcgctaca tcgggtagag atagcggatt
     2821 taatgagtta tcaaatgata ctaataatga taattacatt gccaaataca atcagaaggt
     2881 tataaatcag atggtacgtg cgtatcaata tctatcacaa gctggtcttg ataatccttg
     2941 gaaccctcat ttgacatttt atagatatgt aaattattct ggtttgtcat gggacatcat
     3001 ttcttctcct gtaacagagc atgatagaaa aatttatgat gaatatagga ataaattaac
     3061 taatagcgag ggcctgtata attattttat agatcctaat actggttcaa taataggttt
     3121 gcctcactta attgtgacgt tacaatcaca gatgtttcta gccgacacaa tcaatgatag
     3181 tgtttctgac tttgcaggtt ggttaggaga tttaatgact tgctggggtg aagttaagaa
     3241 attaggaata caaatggagc aaggtgtatt tgcattagta ggtacggctg caggtggaac
     3301 atttagtcta gaagatttat accaagatgt tgacgcggta aatgtttata ccttagcaaa
     3361 aagaaattct gattcaacag ctttatcggc tttaaaaacg tactattgtg ataagggtgc
     3421 tattgataga tttaatcaat ttgttaagaa cagatttggt tcttcagata aaatcgcttc
     3481 agtaacaaat agtttattag aaaatgacga aacagttgat atcttaggtg tagggataat
     3541 ttcatttttc attgataaaa tagttaataa ttcacttggg acagatttta cttatcttga
     3601 ttttattgga gacgagggga aagttgcaaa tggattttct gaaaaaatta ttcaatttca
     3661 agaaaataga agtaaactta attaagaatc gtttactatt taaacagtgt ccttttcctt
     3721 tttaaatata tacgataaaa taagtatatg aattgtaaaa ggacatgatg attatggaag
     3781 aaatagaatg gaataatctg actcaaaata ataaaaataa ttctggatgt ggaattatct
     3841 ttatgtttgt tatagttttg ggaatagtag tacttttaat aactgtcaag atagtagatt
     3901 actttgcgaa tactcctgaa gaacaaatac agagaaaact tcctgagttt gaaagagtac
     3961 caccttatga tccaagagat aattcatttc aagtaaaaaa attaaaagag tctccaaatg
     4021 aaatgtggtt tgaagttaca gttgaatcaa gcgacttcaa agaaaaaaag aaagaataca
     4081 attatattgt aaaggcaaaa gtatcaaaaa gatttatttt taaccctaaa ttagaaatat
     4141 atagtgttgt aatagaataa tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc
     4201 tgatgtgaaa aataaaatat accatggaag acataaaaga aaaactggat aactagttta
     4261 tttaatggca gaccgttaag gcctgccatt tttatgtatt ttagcggaat aaatataatc
     4321 atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc ttgattttct
     4381 ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg atttaatctg
     4441 attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga aaattttttt
     4501 atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc gcgaaggaat
     4561 taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat tttaaacgcc
     4621 ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat ctacaatcat
     4681 tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa ttaactaagg
     4741 aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct agttacaagc
     4801 aactagatgg gttagagacg cttatctctg acttaattct gcaattactc agctatatgg
     4861 ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac
     4921 aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt cctgcgatct
     4981 attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa ttgggcatga
     5041 aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac cacttttctt
     5101 gatagatata acagctatta gacgggaagg gagagtttga aaatggactt aacgaatcaa
     5161 agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat cggtttggtt
     5221 cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt cctggtttgt
     5281 ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga acaaaccgcg
     5341 cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga attttatgcg
     5401 ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt aaaatttaca
     5461 aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga aataactagt
     5521 tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt
     5581 tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt acatggaaca
     5641 tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta caaatcattc
     5701 gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc aatctctgca
     5761 actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg tttttgcgga
     5821 tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc tttttgagcc
     5881 aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg tgcaatataa
     5941 cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt tccagtatta
     6001 aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt cataaaagca
     6061 actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt aacgatgacg
     6121 caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc agtatcttta
     6181 ccactggctt tttcttggaa taatttatca atgttaagtc cttcggttct gttgcgaagt
     6241 ttgaaaatca aacgc
//
LOCUS       DAFFFQ010000113         6184 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000113
VERSION     DAFFFQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.116"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            725..811
                     /locus_tag="LQB69_002705"
     CDS             725..811
                     /locus_tag="LQB69_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL3526816.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="LQB69_002706"
     CDS             complement(932..1621)
                     /locus_tag="LQB69_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526817.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="LQB69_002707"
     CDS             complement(1628..1906)
                     /locus_tag="LQB69_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526818.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="LQB69_002708"
     CDS             complement(1866..2780)
                     /locus_tag="LQB69_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL3526819.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="LQB69_002709"
     CDS             complement(2762..3142)
                     /locus_tag="LQB69_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL3526820.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3603..3890)
                     /locus_tag="LQB69_002710"
     CDS             complement(3603..3890)
                     /locus_tag="LQB69_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3526821.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3910..4134)
                     /locus_tag="LQB69_002711"
     CDS             complement(3910..4134)
                     /locus_tag="LQB69_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBL3526822.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4589..4759)
                     /locus_tag="LQB69_002712"
     CDS             complement(4589..4759)
                     /locus_tag="LQB69_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526823.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4774..5307)
                     /locus_tag="LQB69_002713"
     CDS             complement(4774..5307)
                     /locus_tag="LQB69_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL3526824.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5328..6170)
                     /locus_tag="LQB69_002714"
     CDS             complement(5328..6170)
                     /locus_tag="LQB69_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3526825.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1641 a         1153 c         1042 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtcattcata aacagacaaa atgtcattca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact tttaagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 cttactaaag ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca
     3301 aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc ccccactctc
     3361 cacaatacaa aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac
     3421 cttcggtgaa attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa
     3481 agattcttat gccatttact attaataaat ctataaatat gataattttt cggctctata
     3541 atttatggga ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag
     3601 aattaactcc aaataccaat agaagcccat cctctatata cttgagattc cttactaaca
     3661 atttcttgta attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt
     3721 ccaagaaaac tacttaattt catactaact tcaccaacac tttttccctc actgatttct
     3781 ttagaataaa gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg
     3841 tatactttac taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc
     3901 tccctaccac taccttctag gtgcccatgg accatgatta acccaaccat taacaataat
     3961 ttttccaatt tctcctttag cttgattcca atctacccaa catttttctt tgttacaata
     4021 aagaccattt ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag
     4081 acaagttcct aatattccta gaataacaca atgttttaat actttctttt tcatgattgt
     4141 ttttcctttt cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta
     4201 tataacattt tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat
     4261 cgtttaaata aaattataag atcagttatt taaacagcta gtagacataa tgtacatact
     4321 accaagtaac gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta
     4381 ggttaaaagc tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga
     4441 atattgatcg gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta
     4501 catatctcat attcatttat tattcttttc taaggttaat aataggataa tctctttcag
     4561 aaagactgct cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct
     4621 cattttgact gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt
     4681 tggccactga taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata
     4741 gcaagtgttt ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt
     4801 ccaattcttt gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt
     4861 ctctgaagac tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg
     4921 gttggcttgc agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt
     4981 ttgctctttg agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag
     5041 caacttgttt gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc
     5101 atgtttatgc aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac
     5161 tccagaacgc aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa
     5221 tttttggata tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt
     5281 tattgtaaag gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa
     5341 ttatctaaat cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt
     5401 ttttcaagag cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct
     5461 cgtttcttct gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt
     5521 ttatcagcta aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag
     5581 tcgtctgctt ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc
     5641 cgtccttttc cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga
     5701 cgtataggtt ttataactcg tttatctata tcaggttgct tataactttt aggaatatct
     5761 aataattcaa gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt
     5821 aacagacgaa acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa
     5881 tagcgtaccc actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata
     5941 tctacgtaag gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata
     6001 tcgtaagaaa gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa
     6061 gttgctttta aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct
     6121 tttagttggt caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg
     6181 atgg
//
LOCUS       DAFFFQ010000114         5907 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000114
VERSION     DAFFFQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.117"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(95..667)
                     /locus_tag="LQB69_002715"
     CDS             complement(95..667)
                     /locus_tag="LQB69_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3526826.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LQB69_002716"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="LQB69_002716"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL3526827.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="LQB69_002717"
     CDS             complement(1352..1867)
                     /locus_tag="LQB69_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBL3526828.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="LQB69_002718"
     CDS             complement(1869..2153)
                     /locus_tag="LQB69_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3526829.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="LQB69_002719"
     CDS             complement(2187..3515)
                     /locus_tag="LQB69_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL3526830.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="LQB69_002720"
     CDS             complement(3529..3984)
                     /locus_tag="LQB69_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526831.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="LQB69_002721"
     CDS             complement(3987..>5907)
                     /locus_tag="LQB69_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL3526832.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DAFFFQ010000115         5876 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000115
VERSION     DAFFFQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.118"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            640..1374
                     /locus_tag="LQB69_002722"
     CDS             640..1374
                     /locus_tag="LQB69_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3526833.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1390..2559
                     /locus_tag="LQB69_002723"
     CDS             1390..2559
                     /locus_tag="LQB69_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3526834.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2575..3570
                     /gene="lacD"
                     /locus_tag="LQB69_002724"
     CDS             2575..3570
                     /gene="lacD"
                     /locus_tag="LQB69_002724"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL3526835.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3649..4575
                     /locus_tag="LQB69_002725"
     CDS             3649..4575
                     /locus_tag="LQB69_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBL3526836.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4594..5844
                     /locus_tag="LQB69_002726"
     CDS             4594..5844
                     /locus_tag="LQB69_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526837.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1203 g         1751 t
ORIGIN      
        1 tttgaaaaat gctatttcga ggtagaagga tattatataa gcacggttga ctaaacgaag
       61 tgataataaa aaaagttatt caagaataag aaaaattatg atacgaattc agataaactg
      121 gagaatacag agacctttaa aagggataag aaacaacata aattcctctt aagagagaac
      181 gaaaggacaa agccagcaac ttgatacacg agctagtatt agaggaattt attctcatag
      241 actccctttt gaatacgttt caactttaat tttcatgttc ataaatgatt tgaaaccaaa
      301 gcaaatgcgt tgcacttttc ctccgtcttc cgaaaatcgt tcaattcact ggtaagtagt
      361 catttgatag tttgaggcaa attgttgatt gatgaagggt aggagctaaa aaaagatacc
      421 agttgaagag agaaaaagaa aaaaatagag ggtgttcttg aatgaaaaaa gttggaaaat
      481 tatttttcat ttccttttat tcctattaaa gtcagtgatg tcaatcatat taacaagact
      541 aaaaatgaaa ataaaaaaaa gattgttctt agtattgact cataaaaaat accactacta
      601 tacttacatt gtggttatag ccagttagga gagacatcga tgcaaaatac ctttcaatca
      661 aaatcacttt attatcagct ggtagaaata ttgaaacaac gaatcgaaaa cagtatggtc
      721 gctcatgaca agctgccatc agaaagagag ttgaccagtt tttatggcgt tagtcgcacg
      781 acggtgcgac tagcattgca agaactggaa aaaatgggct acatttatcg acaacatgga
      841 aaaggaacct ttgtttcgga attaaggaca aatgcgactg atctagctgg cgcatatagt
      901 tttaccgaac aaatgcgggc aatgggcaaa catcctgaaa ccaagatatt ggccttcaag
      961 aagattgaag caactaaata tttagcgagt catctgaatg ttcaactagg tgagccttta
     1021 tttcaattag accgattgcg cttagctgat gatttgccaa tgatgatcga gaaatcctat
     1081 ttgccagtga agctatttat gtccctttct caagaaattc tatttctgaa acctttatat
     1141 aatttgtttt cagagaattt caatcaaatc gtacggttgg ctgacgagga attttatgcc
     1201 agcattgtcc agaaagaaga tagtgagttg ctaaatattc cagaaggatc accagtgctc
     1261 catttagttc gctcaaccta taatctcgag aacgaagtga ttgaatttac attaagtgta
     1321 gcgcgggctg ataaatttca ttataagatc aggcatctgc gttccactaa ctgaattgga
     1381 ggaaagaaaa tgtttatgtt ctcaaaagaa gaactacgaa agctaggagc agaaaatact
     1441 acccgagaaa ttcgacagca accggaactg tggcatgaga cgtttgaccg ttatcaagaa
     1501 acagttgcag atttagatcg tttgtttacg gatgcgaaac aacatgctgg agagcgacca
     1561 atccgagtca tttttacagg ggccggaacc tcgcaatatg tgggagatac actaattccg
     1621 tttcttacta gtcaaggaaa tacgaaccag tttatttttc aaagtattgg ttcgaccgat
     1681 atcgttgcta cgccaaaaga atacctgatt cctgatgaga caacgatctt ggtctcattt
     1741 gcccgtagtg gaaatagtcc agaaagtcta gccgcagtta agatggccaa tcagctggtc
     1801 aacaagatct atcatatcgt gatcacctgt gcaccagacg gattattggc tacacaaatg
     1861 cctacaaaaa aagaaaatct agttttacta atgccaaaca aagccaatga cgctggtttt
     1921 gctatgacaa gcagtttcag ctgtatgacg ctgactgcgc tacttatttt tgatcaagca
     1981 agcaatcagc aaaaagaaag atatgttcat caattaagcc gactaggaga agaagtcatc
     2041 caacgagaat cagagatcca agggtatctg gcatcagact ttcatcgcat catttactta
     2101 ggttcgggaa gcttaggcgg attgactaga gaagcgcaat taaaagttct cgagttaact
     2161 gctggaaaaa tcgcaaccgt atttgataca tctttaggat tcagacatgg tcccaaatct
     2221 tttgtcaatg agcaaacatt ggttttcctg tttgtcagca atgactcata tacgcagaaa
     2281 tacgatgtag acatgctgga agaaatcaaa aatgacggta ttgcccaacg cgttgttgga
     2341 attggtcaaa agggttcatt gtttagtggt gattcctttg aattagttgc agattcgtta
     2401 ctgccggatg gctatcttgc tttagcagat atcatggtag cccaaacgat cgcattactt
     2461 tgttcggtga aggttggtaa tacaccagat acaccatcgc caagtggaac agtcaatcgt
     2521 gtggtcaaag gcgtaacgat ttatccatat gaaaaatagg aaagagtgtg aagcatgtta
     2581 gaatggtcgg aaaataaaag acaagcaatg gaacgtctat cgacgccaga aggcattatt
     2641 accgctttgg cgatcgatca gcgaggcgcg ctgaagaaaa tgatgtcggc tttagggatg
     2701 gaagcgacaa aggaaaaaat tgagcagttt aaaacactaa tttcccaaga attgactcct
     2761 tatgcctcag caattttatt ggatcctgaa tacggactgc ctgctgcaca gaagcgtgca
     2821 gcagcgagcg gtctgctgtt ggcatatgaa aaaactggtt atgatcaaac cgtacccggc
     2881 cgcttgccgg atctgctgtc ggactgttca gttctgcgat tgaaagagtt aggtgcggat
     2941 gcggtgaaat ttttactgta ttacgattat gaagaagcag aggaaatcaa tcggcaaaag
     3001 caagtttttg ttgaacgaat tggaagcgaa tgcatggctg aaggactgcc attttattta
     3061 gagttattgt catatgatgc taaaaatgta gatacgaaat cgctttccta tgccaaagca
     3121 aaaccgcgca aagtgatcga aatgatgaaa gaattctcga aaacccagta taaagtagat
     3181 gtcttaaagg ttgaagtacc agtaaacatg tcatttgttg aaggaaatgc aacaggggag
     3241 atcgcctata cacgtcaaga agctgccgct tattttaaag aacaaagcga tgcaacagat
     3301 ttgccgtata tcttcttaag tgctggggta tcagcggcat tattcatgga gacattgcgt
     3361 tttgccaaac atgctggggc acgattcaac ggtgtactct gtggcagagc aacttggaaa
     3421 gatgcagtcg ctcctttcgt tgaaaaagga gaagcggcaa ctttagaatg gctgacagaa
     3481 caagggacac aaaacattcg tcaattgaat gaagtgataa gagaaactgc catcccatgg
     3541 tatgagaaag taagtgaaag cacttgttaa tgtttctaat atattagtag tgataagaga
     3601 tatgtgtgat tataatttat cattattgaa actgggagga tattttatat gaataaaaga
     3661 ttgttagatt attcagcttc agattttttg aaagctaagc cgatggaatt aaagcaggca
     3721 attttggctt ctgaaggtag aacaattatg gcagaaaatg tgccaagtag ctgtccttac
     3781 atgggaaaat cagtgacaaa tgcagaattg gagagagctg ctggagctga tttaattttg
     3841 tttaatgcac tagatctttt taatcctcaa atttcaggtg tacctttaac tctaaaagag
     3901 aatccaataa tctggattaa aaaagcttta ggaagagcta ttggtgtaaa tttagaacct
     3961 gtagacaaag aagttttgat gagtgaaaat cgaactgaaa tttcaagtgg tagaatcgtt
     4021 tctcctgaga ctttaaaaaa ggcaaatgaa ttaggcttta attttatttg tttaactgga
     4081 aatccaggaa caggagtatc taatagaact attgagaaag caataacatt atccaaatca
     4141 atttttaatg gattaattat cgctggaaaa atgcatggtg ctggtgttga tgaaccaatt
     4201 atgaacttag atatagcaaa aagatttatt aaaacaggtg tagatatttt attagttcct
     4261 gctccataca ctgttccgta tttcatggaa agtgatttgc gaggaatcgt tgattatata
     4321 aaagatttta atagaaataa atctattgag gaaaaagttc tagtactaac ggctaatgga
     4381 acaagccaag actcttctga ccaagaaact attaaaaaaa ttgcattagc atcaaaagca
     4441 tgtggagctg atattcaaca cataggggat tctttaaatg gtatttgtct tccagaaaat
     4501 atttttgctt tgggtcaagc aattcgggga tatcgtcatc aaattattat gttaggaaaa
     4561 tctaattatc gttaattaag gaggaaatga aagatgatcg acaaagttca aaaaatagct
     4621 gcaaaaatta gtaatcaaag acatctacta gctttaagag acagcatggc tgcagttatg
     4681 ccattaatca ttattggttc cttttttatg ttattagcaa atttcccgat agaaggattt
     4741 acaaaatggt tatctgaaat tggagtacaa tcctttttga acaaagcatc agattcaaca
     4801 tttggtatta ttggattagc tgttacctac tctttgggac acaatctagc aaagacttat
     4861 ggtgtagact cattatcagc aggattatta tctttgagtg gatatgtttt acttacacct
     4921 ttattggatg cagatggttc tataggattt ccaacaaaat actttggtac ctcgggtctt
     4981 tttgttggta ttattgtagc acttgtatct acagaaattt tccggtggtt tgtacttaaa
     5041 aatattacaa ttaaaatgcc ggcaagcgtt ccaccaaatg taagccgagc tttcgttgct
     5101 attattcctg gcttttttgt cgttctacta tggtttattg ttgttgtaat ttgttataaa
     5161 cttggaattg aaaatgttca tgctcttatt gcagatacac tcgcaaaacc actatcttta
     5221 ctgacgaaaa ctttaccagg aattatattg gttatactta ttcaatgttt cttttggatg
     5281 tttggtattc atggtgcaca ggtaacgggg ccaataattg aaccgttatt attgcaaaac
     5341 tctgatgtaa atagaattgc ttatcaagct ggaaaagaac ttcctaacat tataacttat
     5401 gaattcttat ataactttgt tttttctggt ggtgcaggtt gtttatttgc acttgccatt
     5461 ttaacatttt tcttttcaaa gagtcaggaa aataaaactt taggtaagtt atcaatcgct
     5521 ccagtaagtt ttcaagtagc agagcccctt ctatttgggt ttccaactat attaaatata
     5581 aaaatgttaa ttccatttgt aactgcacca gtagtaacaa cattaattac ttatttttca
     5641 atgagtatgg gattagttgc aaaacctgta ggagcaacaa tcccatggac aactccaccg
     5701 ataattgccg gatttttagc ttctggtgga cgaataagtg gtgcagtgat tcaagtaata
     5761 acaattgcaa taaatgtttt gatatactat ccattcttta aattagatga taatgccaag
     5821 ctaaaaagcg aaaaaaatga ttagaactac ctatatgttt ctaactgtag ttaagt
//
LOCUS       DAFFFQ010000116         5707 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000116
VERSION     DAFFFQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.119"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..193)
                     /locus_tag="LQB69_002727"
     CDS             complement(<1..193)
                     /locus_tag="LQB69_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526838.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(736..2886)
                     /locus_tag="LQB69_002728"
                     /pseudo
     CDS             complement(736..2886)
                     /locus_tag="LQB69_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3032..3607
                     /locus_tag="LQB69_002729"
     CDS             3032..3607
                     /locus_tag="LQB69_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526839.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3821..4516
                     /gene="vanR"
                     /locus_tag="LQB69_002730"
     CDS             3821..4516
                     /gene="vanR"
                     /locus_tag="LQB69_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL3526840.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4494..5648
                     /locus_tag="LQB69_002731"
     CDS             4494..5648
                     /locus_tag="LQB69_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL3526841.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1784 a         1096 c         1083 g         1744 t
ORIGIN      
        1 gttcaaaatc taaaatcaac acatcatggg gagtgtgata ataaatattt tcccttaatg
       61 taccaacaat ggtaattcgg tcaattgacc attgcatttt taagggggtg cacatagttt
      121 gtgcatttaa ccgcctgtta gaaatacggt taagtttttc ccactcattt acgattatgc
      181 cttttttact caaaaatagc tcctcctcaa cgaaaaaaga gattcttgga aagaatttcc
      241 agagttcacg taactctaaa aatccacatc caaaaatctc tagcttgaag aaacctaaaa
      301 atattcgtat aatgaacttg agttacggaa ctaggtgaag tagtttccgt aaaggtcgag
      361 agtgcgtcaa cactgctcga ctattttttt actctttttt ctcattttct ttgattatag
      421 cactcctcta gaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc
      481 tcctcctgcc gaaatagaat ggcgctgcca gcatttattt cgttcagcta tcggctacac
      541 aatctaacac gaccgttccg ctaccagtcg ccttacaggc tccattttcg ctcctctaac
      601 gtgtttcacc gattgtgccg ataactaaac gaaataaacg ctaaaaccgc tcagaacgca
      661 aaatatgcca ttttagagct attgtagaaa atatagtttg aaatgcaatt ttcttctttc
      721 agctttttat ttcccacttg taaatcggta gatggtggct ccttttccag ttccataatg
      781 tggatttgca tctgcatgta gtgatgaaac acctagctgc attctcatac catctgacga
      841 agatgttgta ccgtcgcccc aatagaaagg caattgtaat ttatgatgaa agtttactaa
      901 tatggcttgg gctttattca tggcatcttc atacatgcgc cattgagata cattggctag
      961 ttgcttatat gtaagtccgg gtgtggcttc ggccatcttg ctcaagccaa tattcattcc
     1021 cattcctaaa agggcagcca tgataatgat tgtttcttcc ttatctggtt ttcgattatt
     1081 ggaagcatga gtgaattgct catgaaatcc tgttatatgg gccacatcca tgagtaaatc
     1141 agttaatttt attcttggta gcatctgata aaggcttgca ctaaattttt ttgcttcttc
     1201 tggaacatct ttttctaagc gtgcaagtga tagctttcct ttttcaagag aaaccccatc
     1261 taacttattg gaattggcag ctaaccactt taacctttca ttaaagctgc tggttctctc
     1321 cgttatataa tcttcgaatg ataaactaac tgataatctc gtattcccct tcgattgatt
     1381 ccatgtatct tccgaaaaca aatattcctc aaaatcccta tattgtctgc tgccaacaat
     1441 ggaaacatct cctgcccgaa catgctcccg aagttctgtt aaaacagcca tttcatagta
     1501 atgacgatta attgttgtac catcatcctc gtataaatgt cttttccatc gttttgaaat
     1561 aaaatccaca ggtgagtcat caggcacttt tcgctttcca gattcgttca ttcctcggat
     1621 aatctcaaca gcttgtaaaa gtggctcatt tgcctttgta gaatgaaatt ccaatactct
     1681 taatagcgtt ggcgtatatt ttcttagtga ataaaaccgt ttttgcagta agtctaaata
     1741 atcatagtcg gcaggacgtg caagttcctg agcctcttct actgaagaga caaaggtatt
     1801 ccattcaata accgattcta aaaccttaaa aacgtctaat ttttcctctc ttgctttaat
     1861 taatgcttgt ccgatgttcg taaagtgtat aactttctca tttagctttt taccgttttg
     1921 tttctggatt tcctcttgag ccttacgacc ttttgataac aaactaagta tttgcctatc
     1981 atgaatttca aacgctttat ccgttagctc ctgagtaagt tgtaataaat agatggttaa
     2041 tatcgaataa cgtttatttt cttgaaagtc acggaatgca tacggctcgt atcttgagcc
     2101 taagcgagac agctgcaaca ggcggttacg gtgcaaatga ctaatttgca ctgtttctaa
     2161 atccattcct cgtatgtatt cgagtcgttc tattattttt agaaaagttt cgggtgaagg
     2221 atgacccggt ggctctttta accaacccaa tatcgtttta ttggattcgg atggatgctg
     2281 cgaggtaata atcccttcaa gcttttcttt ttgctcattt gttagagatt tactaaccgt
     2341 attaaatagc ttcttttcag ccattgccct tgcttcccac accattcttt caagtgtagt
     2401 gatagcaggc agtataattt tgttttttct tagaaaatct atgcattcat gcagtagatg
     2461 aatggcatca ccattttcca aagctaattg atgaaggtac ttaaatgtca ttcgatattc
     2521 actcagggta aaagttacaa agtcgtattc acttcgaatt tctttcaaat gatcccaaag
     2581 tgtattttcc ctttgaggat aatgatcaag cgaggatgga ctaacaccaa tctgtttcga
     2641 tatatattgt atgaccgaat ctgggatgct tttgatatga gtgtatggcc aaccgggata
     2701 ccgaagaaca gctaattgaa cagcaaatcc taaacggttt tcttccctcc ttcgcttatt
     2761 aactatttct aaatcccgtt tggaaaaagt gaagtaggtc cccagtatcc attcatcttc
     2821 agggatttgc ataaaagcct gtctctgttc cggtgtaagc aattctctac ctctcgcaat
     2881 tttcattcag tatcattcca tttctgtatt ttcaatttat tagttcaatt atatatcaat
     2941 agagtgtact ctattgatac aaatgtagta gactgataaa atcatagtta agagcgtctc
     3001 ataagacttg tctcaaaaat gaggtgatat tttgcggaaa atcggttata ttcgtgtcag
     3061 ttcgactaac cagaatcctt caagacaatt tcagcagttg aacgagatcg gaatggatat
     3121 tatatatgaa gagaaagttt caggagcaac aaaggatcgc gagcaacttc aaaaagtgtt
     3181 agacgattta caggaagatg acatcattta tgttacagac ttaactcgaa tcactcgtag
     3241 tacacaagat ctatttgaat taatcgataa catacgagat aaaaaggcaa gtttaaaatc
     3301 actaaaagat acatggcttg atttatcaga agataatcca tacagccaat tcttaattac
     3361 tgtaatggct ggtgttaacc aattagagcg agatcttatt cggatgagac aacgtgaagg
     3421 gattgaattg gctaagaaag aaggaaagtt taaaggtcga ttaaagaagt atcataaaaa
     3481 tcacgcagga atgaattatg cggtaaagct atataaagaa ggaaatatga ctgtaaatca
     3541 aatttgtgaa attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa
     3601 taattagcca ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat
     3661 aaaatattaa cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat
     3721 cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga
     3781 taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat
     3841 tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta
     3901 tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat
     3961 tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa
     4021 aataagggac aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga
     4081 taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact
     4141 ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa
     4201 ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga
     4261 gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat
     4321 cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg
     4381 cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga
     4441 aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta
     4501 taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata
     4561 tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag
     4621 ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg
     4681 acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg
     4741 cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact
     4801 ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc
     4861 tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa
     4921 agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg
     4981 cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg
     5041 ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt
     5101 atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga
     5161 taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat
     5221 tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga
     5281 ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg
     5341 ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg
     5401 tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg
     5461 aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat
     5521 tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg
     5581 ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga
     5641 ggtcctaaga gatgtatata attttggctc tttgtcaaat gatgttggta aagagaaatc
     5701 ttagaat
//
LOCUS       DAFFFQ010000117         5467 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000117
VERSION     DAFFFQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.121"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            119..1108
                     /locus_tag="LQB69_002732"
     CDS             119..1108
                     /locus_tag="LQB69_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL3526842.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="LQB69_002733"
     CDS             1090..1551
                     /locus_tag="LQB69_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL3526843.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="LQB69_002734"
     CDS             1601..2065
                     /locus_tag="LQB69_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL3526844.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="LQB69_002735"
     CDS             2079..2828
                     /locus_tag="LQB69_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL3526845.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="LQB69_002736"
     CDS             2828..3658
                     /locus_tag="LQB69_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL3526846.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="LQB69_002737"
     CDS             3658..4305
                     /locus_tag="LQB69_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL3526847.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="LQB69_002738"
     CDS             complement(4403..5179)
                     /locus_tag="LQB69_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL3526848.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5261..5404
                     /locus_tag="LQB69_002739"
                     /pseudo
     CDS             5261..5404
                     /locus_tag="LQB69_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          740 c          973 g         1882 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ttaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaga tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaata aaagattaag atataaatat aaatgaacta gtctttgact aaccaatgcc
     5281 cgcttttgaa agaaaacaaa ttcattctgt tttaagtaaa aatccttatg ttaccactca
     5341 ttctgaaggg gatgagcctt atcgctactt ggttgtggaa ccaagcgata agacaatttt
     5401 ataaattcaa tattaattaa aataaaaaca aacaaagtag attattttac tttgtttgtt
     5461 tttttat
//
LOCUS       DAFFFQ010000118         5427 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000118
VERSION     DAFFFQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.122"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(126..772)
                     /locus_tag="LQB69_002740"
                     /pseudo
     CDS             complement(126..772)
                     /locus_tag="LQB69_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(1180..1683)
                     /locus_tag="LQB69_002741"
     CDS             complement(1180..1683)
                     /locus_tag="LQB69_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL3526849.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(1806..2570)
                     /locus_tag="LQB69_002742"
     CDS             complement(1806..2570)
                     /locus_tag="LQB69_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL3526850.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            2678..2953
                     /locus_tag="LQB69_002743"
     CDS             2678..2953
                     /locus_tag="LQB69_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL3526851.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            3391..4341
                     /gene="yidC"
                     /locus_tag="LQB69_002744"
     CDS             3391..4341
                     /gene="yidC"
                     /locus_tag="LQB69_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL3526852.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(4520..4720)
                     /locus_tag="LQB69_002745"
     CDS             complement(4520..4720)
                     /locus_tag="LQB69_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526853.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         1584 a         1135 c          943 g         1765 t
ORIGIN      
        1 aaaggggctg tccccagact ttagtaggcg tcattagcta tctttcgtta gcatttaaag
       61 gcgaacacca tcacctattc acttccatta aaagatagca tatttttctg taaaaaacaa
      121 taaaattatc gttctttctt tacacgttca gcaaattcat agaaaggagc caatttcgtt
      181 aactgttgat tacattggtc actatcatga caaatataat tccccttttt cgtatacgtg
      241 ccatcagaac ctgctttcgt tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag
      301 attgcacaga ttcccttatt tatcgttggt gataaagtac cgctaatccc ttttaatttt
      361 tcatcttcgt aatagaccat gaattttttc tgcgtacctg gatcattcca gccgatgtac
      421 gtatgttctt gcaagtcgat ttcattccaa gctggttgtt tgagtttttt cacttttcgg
      481 aataatttct cgatttgtgg tttgctaggc tgcataaacg gctgaacgat ttgcttcagc
      541 tcctccaatg ctctttctgc ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa
      601 aatgtatcga ttctggcagt tcgtgatcta atatgtctcg aatcttttca gctgctagtg
      661 cctgaactgt ttgtaccgtt tttggatcat tgacagatga gtacgcattt aataactgtt
      721 ctacttctct tattataaaa aaatattggt atggttttat tgtttgttcc atgattttaa
      781 agtcctttct tctaaccaaa gtgcatactt tgtagaaaaa agtgatcttc tgcaaagtat
      841 gtgttacctg aagatcccgg actcttttaa ataagatacg ttttgcatct tatcatcacc
      901 ccctttaatg cttattattc tatgtacatg attcattcga taaataaaag taaaccgtat
      961 cttgaacaga tagtcaactg caaacagaaa aaaggactgt gacaaaagtc gtgtcaaagt
     1021 cccttgtttc gaatgaatgg tgttcaaaag ctggcatctg cggtaataag ccgaaacttc
     1081 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
     1141 tgtcacaacc tcaatatggt ctattattgg ttttatctat taaatcgagg attctttccc
     1201 aaagtaacgt tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac
     1261 tgcgcagtat ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat
     1321 cgttgcgccc cacatgtgct tcaaaatgat atctcgctca agatccgaca attcggtgat
     1381 cttttctgca ttctttacag caattcttgg atggatatac gcatgtgtgc cttcatcaaa
     1441 tttcgtcgta cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc
     1501 tttagcattg ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga
     1561 atgctccaaa cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc
     1621 tgtttctaaa agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt
     1681 cataattatc gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat
     1741 accaccagtg ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc
     1801 cttaatcata aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat
     1861 tccgcatttc ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga
     1921 cttaccccct gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg
     1981 tctaatgcgt gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc
     2041 attaaaggaa tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc
     2101 tgcatgctgc gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca
     2161 ccagccatat caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt
     2221 acattatcta acaacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc
     2281 atcactgcta agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc
     2341 gaaactagaa aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg
     2401 cctcgctgtg tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc
     2461 agatggaacc cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt
     2521 ttttttaatt ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc
     2581 tccccatgat tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta
     2641 tcattaattt ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt
     2701 ccaaggacgc gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact
     2761 aggcgtgact ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg
     2821 caatgacgac atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg
     2881 acgagtgcag tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga
     2941 tgtggtggga taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga
     3001 aaaatagctc tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga
     3061 acaccgtcga taaggttatt cttgaggcgc tttgcctcaa aaattaagga cagcagtgaa
     3121 aaagctgatc tatcacttgc agtgagttca tttgactccg ctttttcggg cagaaaaaaa
     3181 gtttttagtt tattaaagta agaaagaaac acttggaatc attctacttt ttcctagtat
     3241 ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag aacgaatatt
     3301 tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat agttaagttt
     3361 gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg gttattggga
     3421 tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga ttcaaacgga
     3481 aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg ccaagcgatt
     3541 acatatctag ttgaaaattt caattggtct tatggctggg cagttattgc aatgactgtg
     3601 atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac aatgatccaa
     3661 tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa attgaaagct
     3721 gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt ataccgcgaa
     3781 aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca aatgccgatc
     3841 ttctctgcct tgtactttac ggcacgttat acagaaggta tccgggaatc atctttctac
     3901 ggcatcgatt taggctcacc aagtatggta cttgttgcca ttgcaggggt cgcttatctg
     3961 cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac gatgcgtacg
     4021 atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc agccggtgtt
     4081 acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat cacaaacgtt
     4141 atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa cccaccaaaa
     4201 caagtcgtca ctccaagaat tgatgtgaca gaatcagcta aaccagctga cagcaaacaa
     4261 gcaaccaaac aaatcaagca acccaaaaac agtacaaaca ataaaggccg caacgcagga
     4321 aagcagcaga agcggaagta gatagtaaat aagagtgtaa taaaaattca ttaacagttt
     4381 tagcaattaa aaatatcgac caagattttt gaaaaagaaa tctggtcgat atttttattt
     4441 aagtgaatgt ttaatttgaa tcatcgattg atcgctatct aattgcagca gcatcttttt
     4501 atgaagaaac aactttttct tatctatcaa cgtagacagg tgttgctgtt tcttctaata
     4561 gtgtacttaa ttccttaata ttttgttttc cttcttctaa catccaacta ctcgcttttt
     4621 cttcgccttc ggtaacaaaa ggcgccactc ccttcgccca tgttgctcgc ccacataaga
     4681 caccattctc cataaaggac aacgcggaga taccacccat ctaaataaca tccataaacc
     4741 tttctagtag aagaatagtg atagtatcca tccctttttc tactgggaat acaattacac
     4801 gattcaaagc ttgatacaca acatttccct tcttataaac cgctattgta accgaattgt
     4861 tttgaatcac ctttttggga tagaaactgg ctcctttccg caacttctta aatgttggtt
     4921 tcttttatac tttctttgta gcaactacaa atttcttcaa atctctaaat gtttgcataa
     4981 caaccgcctt tctgactttt actcaggatg gtatttaaaa atcagcaaaa taaggcttct
     5041 ctccatcttt tttatttcct agctgcttta aagacactct tgctgttttt taatctctat
     5101 caagggggct ctgaatctat tttgatgaac ttatcttatt aatttctcaa atgagctgtc
     5161 agacacaact gtattttttc aatctcatac cggacaacta tgccagctta aaaaaatttc
     5221 ctctggcaaa acttctttat tgaaaaatga caatgaaaaa agaccattct ttttgttgaa
     5281 tgctcattgt gtaaacatat ttcctgcgac tgctcttcga accattctca gtgcaatccc
     5341 taatttatct attaaatctt cttttagttc ctcatctttc atattcaaaa tttcctttgt
     5401 atgttacaca agtttgttct ctaaata
//
LOCUS       DAFFFQ010000119         5339 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000119
VERSION     DAFFFQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.123"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(62..280)
                     /locus_tag="LQB69_002746"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="LQB69_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="LQB69_002747"
     CDS             complement(546..740)
                     /locus_tag="LQB69_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526854.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="LQB69_002748"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="LQB69_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2092..3132
                     /locus_tag="LQB69_002749"
     CDS             2092..3132
                     /locus_tag="LQB69_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL3526855.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="LQB69_002750"
     CDS             3956..4288
                     /locus_tag="LQB69_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL3526856.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="LQB69_002751"
     CDS             4907..5248
                     /locus_tag="LQB69_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL3526857.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaagga
//
LOCUS       DAFFFQ010000120         5254 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000120
VERSION     DAFFFQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.124"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..487
                     /locus_tag="LQB69_002752"
     CDS             <1..487
                     /locus_tag="LQB69_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBL3526858.1"
                     /translation="KKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPK
                     YKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYL
                     YGGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMI
                     V"
     gene            complement(989..1405)
                     /locus_tag="LQB69_002753"
     CDS             complement(989..1405)
                     /locus_tag="LQB69_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1407..1688)
                     /locus_tag="LQB69_002754"
     CDS             complement(1407..1688)
                     /locus_tag="LQB69_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526860.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2064..2588)
                     /locus_tag="LQB69_002755"
     CDS             complement(2064..2588)
                     /locus_tag="LQB69_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL3526861.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            complement(2605..3573)
                     /locus_tag="LQB69_002756"
     CDS             complement(2605..3573)
                     /locus_tag="LQB69_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL3526862.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            4677..>5254
                     /locus_tag="LQB69_002757"
     CDS             4677..>5254
                     /locus_tag="LQB69_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBL3526863.1"
                     /translation="MVGLFFYIFLRKKILMKTSNRKYKVKTVNHRIPPKKALCVINNS
                     KVINQELKPYLSEQEMIKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDK
                     VTILDVIETYKLDSFIRNQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDK
                     NIYSDLKWAEDIIGHFNSFFYSNQSPTFKHHH"
BASE COUNT         1634 a          815 c          895 g         1910 t
ORIGIN      
        1 aaaaaaaaat cattgtttac caatatgggc tttatttgaa gaaataacat ttggtagttt
       61 aacaacattt attaatcaac ttaatacaga gtattataat aattgggtca tgagctgtta
      121 tgataatcct aaatacaaaa aagctatacg aagttgggca aacgtaatta gagcttatag
      181 aaataagata gctcatcatt caagaatata tgcatttaaa gttactgacg ctccaacaat
      241 cataaaaaat gattccaaaa gttattttcc tgaaaataac ttaaaagaaa cccaaaagtt
      301 atatttgtat ggaggattat acgttataaa acatctgtta atatatgaag atagcatgac
      361 acaaacttta tggaataaat ttctaataga tttagaaaaa aagatcgata aaatcccttc
      421 cttagaaaaa tccaattatg gttttccgga aaattggaga aacaagttaa aaataatgat
      481 tgtttaattg ttatttaaat tgttagtaga cataatgtac atactaccaa gtagcccctg
      541 gatcaaccga tccaggggaa gactttgtga ttttcaattg gtctatctaa aacatctttt
      601 taaaagcttg attcttcgct gttggctttt ttcgattctt tgtgaatatt cagtgattat
      661 ttttttacta cttgttattt tccgagggat ttgttaaaag tttaatagcg tttctcagta
      721 agggacgtcg ttttttcatt ttcctatgta tggactgctc gaacgagcag ccttttttta
      781 ttccagggaa cgtcctattc tttgagtttc catgctattg aataaccggg acttagggat
      841 tagggcagcg ccccaaaatg aaaaaagaaa aatccgcttc tgcgaattct tctttttttt
      901 cgcaagttgg taccaacttg caaatcttgc tggaagaccg atccctaaaa agaaatcgag
      961 gaattttttt gtttttcggt ttcttttttt atctttttag aaagtttgtt gggtcaaaat
     1021 ttccagaagt aaaggtttga ttggtttctt tttgacgatt tttgggttga tttcgtcggg
     1081 aaggattgag ttgattttca attttttctt tcacttcttg atatacttta gaatttgtac
     1141 cgataagaac accagtatct tttagatata aatcagcaaa ttctgccatg atttctgtca
     1201 tgatagaaat ttcttttccc atggcattga gttttgcttg gttactggtt gtggttgtat
     1261 tggtatcagg ttcataactc atacataaaa agcgaatggc ttctgaataa ttgttaacgt
     1321 ctggattttt ttcgatgatg gaatctaaaa cgtcaacgtg actgggataa agggttacgt
     1381 gtttttgaac agaagttttt ctcatgttat attcctccga taagtgtttc aatgagttgg
     1441 ttgtttgtgt gaacatagtc atttagtatg ttcgtatttt ctgcttgaat ggcaataaat
     1501 ttttcgaata ataaatcgaa ttgatctttt ttgtaattca taagttcgtg gtcaaaatcg
     1561 ttttcgatta aaatttttag gtattgattt cgactccatt ttttgcccgt tttggttgtt
     1621 agttcctgac aacgtttatc tattcttgta ataattttgg gattgatatt tcgaactaaa
     1681 atgtccatgg aaattttcct ttcgtatcag tgtataattt atgtatatca tacaaatata
     1741 gtatttgtat agctattttt cgccaaaaca ctatacatag cctagccaca atggcaaaaa
     1801 agtgatagca aatttttttc tgagaaacgt gataggcaca gcttatctag cacctttcgt
     1861 aagaaagtga taccacgttt ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa
     1921 cgagtgcagt ccgagtaaga ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc
     1981 gcaacatggg cccatgttgc tctgctcgca aaagaaaaaa gaagaataaa accagcaatt
     2041 tttattaatt taaattgctg gttttattgc cagaattgcc accatttttt ctccgtcttt
     2101 gattgttttt ctatcgattc tgtttcttca gaaagagtcg ttgatttatt ttccgaagat
     2161 tcttccgtat aagtcaactg tagttgttcc tgcagtttct cgatttgttg gttggcttgc
     2221 agggtgagtt gttgttgttg gtcgagtaat ttttgcattt gttttatttg ctcgtctttt
     2281 tcttgtagtt gcatatcttt ttgatttaac tgttgttcta ggagagaaag gacttctaat
     2341 tcgggaactt ggttggcgtt tttgtggttt tcggttttag ttttttcgcc aactagttgt
     2401 tgatttgagt tttcgttttc gttaaaaaac attgatttta tagccttttg tccggtttca
     2461 ttaatcacaa acttgtttcc gattttcgaa aaccaaagtt ttcgattttc gtcagaaact
     2521 tttttgttaa ttgctgtttt actaactcct aattcttcag caagttcttt gattgttttt
     2581 tctgtcataa aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa
     2641 gtcttttttt agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc
     2701 tttattttgg gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt
     2761 tcgcttttgt tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg
     2821 ttggttagct agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa
     2881 gtcgtctgat ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc
     2941 ccgtcctttt ccgtatctct tttggattgt cagacctttg aagattgcgg ttagttcttg
     3001 gcgaataggc tttataattt ttttatttat atcactttct ctataacttt tagggatatc
     3061 aagtaattca tgaaagtctt cttttgaaaa ataggcaatt cctttggttc tgtattgttt
     3121 taataatcta aacatggttt tggaataact gctttctaat tcattgaatt gttggagaga
     3181 atatcgaacc cattcttcaa gattattaag taagggtaag gctttctcat ggacttgtat
     3241 ttcagcaaaa ggaatatcag cttttccatc gattttaaat tgattaaata aaacaaatcg
     3301 ttcacgttgc aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata
     3361 cgttgtttct aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc
     3421 ttttaattgt tcgaatgtaa attgtacttt tttatcgcct acgtcacgca ttctagatac
     3481 tatagagaag aataaattca tttctactga cgtaaatttg cgtaaaggaa tcgtatttaa
     3541 ttcatggtgg tatttcacaa tttcatttgc cataaaatcc tccttcaaga ttaactactt
     3601 aacaaaatta taaagagctt tctaataggg gtcaagcccc taattaaaga cccctaaaaa
     3661 cagacaaaaa gacccccaaa aacagacaaa aagaccccta aaaacagaca aaaagacccc
     3721 taaaaacaga caaaaagacc cccaaaagat tctgcacacc ttgggagagt aagcatacag
     3781 ccgacggtaa agaggtttaa aaagaggttt aaaaagagat tataaaagag gagaggggca
     3841 aaatttcccg cctaaaaatt ttctgctttt ttagccaaac aggttcgcga attttactaa
     3901 actgggaaac ttgctccctc tggtcgctaa aaaggtgtga gggaaaaaca ccctcacatt
     3961 gtacctgcgc agtcgcctaa aggctccttt gctaaaaacg ttgtttaaag agattttttt
     4021 agaggctctg cgcgctcgtt taagccgttt taagacagtt tagtatggat aacccttatc
     4081 ttgttcttaa agcaccttac aggcggtcag ggcaagccct aacaaccctt aaagagcata
     4141 aaaccatcct aaaattcgat ttaatctgtc ttatctatgt tcttgtatat tttatatacc
     4201 ttgggtagta aaacgtctga aaacgcaaat aagagcgtat gaaacaaaac aataagcaac
     4261 cgattcagca gaattccgga ggagccattt tcgtacgtta gcaaaaaagt gctgttgcta
     4321 aaagctgctt gcggtgcgga gcgaacaagg tctcctagag cgagacgtgt acaaacaagc
     4381 ttacaaattc gtgaaaggca ccggttttgc gccatttcca agcaaaaaaa ggtctatcga
     4441 gcttgctcga tagaccgatc gcatttagcc ccccgcattc taacaggggg ggaataatgt
     4501 gaaaaattat ggtagtaatg cttgatatat aggcatttct tttggttgca cacttaaaaa
     4561 aaatcacgat tttgacaact aaatttttaa ataaaaaagc tttacagttt aattcaaatg
     4621 tgttttaata tatctatcag tacttgccac gcctctgatt taatatcatg cgcattatgg
     4681 tagggctttt tttttatatt tttttaagaa agaagattct tatgaagaca tcaaatagaa
     4741 aatataaagt taaaacagtt aatcatagaa ttccacctaa aaaggcatta tgtgtaatta
     4801 ataattccaa agtaattaat caagagttaa aaccatattt atccgagcaa gaaatgataa
     4861 aaaaaataag taaagatatt tttttaacaa attccgatac aattttatta gaaaagtttt
     4921 taaagaaaaa tagttatttt cgatttagta tttatgttaa attaatgaaa aatatagata
     4981 aagttactat tctagatgtt attgaaacgt acaaattaga ttcattcatt cgtaatcaat
     5041 tgcattattt tgtgaatcaa atagaaattt tttggaaaaa atctctttca gataatatgt
     5101 gcgtttctta tgaagaaact agcatatttc cgaagaatca gtgttatttg gataaaaata
     5161 tttattcaga tttgaaatgg gctgaagata taattggtca ttttaatagt tttttttatt
     5221 caaatcaaag tcccactttt aaacatcatc atat
//
LOCUS       DAFFFQ010000121         5173 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000121
VERSION     DAFFFQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.125"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..410)
                     /locus_tag="LQB69_002758"
     CDS             complement(<1..410)
                     /locus_tag="LQB69_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3526864.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
     gene            complement(492..842)
                     /locus_tag="LQB69_002759"
     CDS             complement(492..842)
                     /locus_tag="LQB69_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBL3526865.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="LQB69_002760"
     CDS             complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="LQB69_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL3526866.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1481..2146
                     /locus_tag="LQB69_002761"
     CDS             1481..2146
                     /locus_tag="LQB69_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL3526867.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2136..2591
                     /locus_tag="LQB69_002762"
     CDS             2136..2591
                     /locus_tag="LQB69_002762"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBL3526868.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2741..4120
                     /locus_tag="LQB69_002763"
     CDS             2741..4120
                     /locus_tag="LQB69_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBL3526869.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4257..5099
                     /locus_tag="LQB69_002764"
     CDS             4257..5099
                     /locus_tag="LQB69_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL3526870.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
BASE COUNT         1636 a          907 c          981 g         1649 t
ORIGIN      
        1 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
       61 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
      121 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
      181 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
      241 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
      301 gtttgtcgag aacgttgtac tagatgggct acttttgaaa caaagtgtga ttgattgaaa
      361 taagcttcta tcatcacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
      421 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
      481 ctagcttaat tttagaattg gcgtatgctt aaaatgcggt tataggcttt tgtatgaacc
      541 atttcagtaa attgaaaatt atttatgatt gcaatctctt gttgggaacg tgcactatct
      601 cgaataattt ctcctaattc attggattga taggtagcga tattagtcaa aagaatcaac
      661 aagcgattga gctgctcttg ttcttgtaat tgtaaatttg cccattttga tcgatcattt
      721 tcgatgggga cacgtgtatc caaccaaaaa agtgaggttg ctctttccca tacaacgttg
      781 tctaattcat cctcaatgac attccagtta attgctttat agtgtaaata attatttatc
      841 atagtacctc ctaaaatatt gtttgaaagg ggatgatatg gtgtcatttt ttattgcatt
      901 gtttctgcaa ttctctctat atcttctgtc gtacctttta gttcaaattc atccaacacg
      961 gtaaaaccaa aatcatcggc gtattgatag gctgttaggc aaaattgttt attgaagttt
     1021 ctgttgccac taccaataat tcctaaacaa tagtctcggt ttttattgta agtaatcact
     1081 tggcgcaatg gtgttgtcaa aatttcgaca tctcctgttt caataccatt tccaccttca
     1141 agataagtag gcagaaatac aaaataggat tcattgatgt caaaagtgag tggttttcct
     1201 aatttgacta aatcttttac attcacataa tctaccttct tttgatattg tttttgacaa
     1261 tacgtatcta aatctgcgag aaaactcgtg gtatttccac ttaaactaat ataatagact
     1321 ttaattattt gcatgtatca cacctctttt taataaataa ccacatatat agtgcttgtt
     1381 ttaattagaa acgctatata tagtgtttat tgtacatgac atatcgttat agaaaattga
     1441 cgatggtcaa ttattttgat tttagaggag agaaaattac atgaaaaatt caggttgttt
     1501 atgcaccgtt ccactcttcg cctctctttc attagatgaa aaaaatgaga taaagtgttt
     1561 gcttcaccat cagacgttta aaaaaggaga aattattttt tcaccaagta gtgaagaaca
     1621 actgttgatt atatctttag gggctatgaa aatatatcga attagtagaa ctgggaagga
     1681 gcaattaatt cgtatcgtaa aagaagggga ttacgacggt gagaactatc tctttggtct
     1741 acccaatgac tgcctatatg gtgaagctgt tgaagaaacc aaaacttgca ctctctataa
     1801 atccgacttt aataaagtga tggcacgcta tccacagctc agttatcgat tgttacaatt
     1861 aaatgcttgc aaaacagtag ctgtggaaaa acagttgcag cttctagcgt ttgatcgtat
     1921 agaggaccgg ttagccttat atcttaacca actatctata gcagcaaaaa tgggggagcc
     1981 tattacttta cctatctcta tgagagagtt ggcaaactat ctagctacca gtcctgaaac
     2041 tatttctcgt aaattaagag attttcaaga aagacaattg attactagaa aaggaaagca
     2101 gattacacta ttagaagaat tttggtttat atttaattat atataaagaa gaagatatta
     2161 aaattgggat tgtcattgca cgattcaatg attttttaac taaaagctta ctaagtggtt
     2221 gtgaagatgg ccttacgcgt tcaggcgttt tgacagaaaa tatcgatgtt gcttgggtgc
     2281 cgggggccta tgaaatccca cttacagccg caacgatggc tgaaacaggt aaatatgatg
     2341 ccattattac cctaggtgcg gtaatcagag gagctacaag tcactttgat gtggtggtta
     2401 accaagcgtc aagtggggta tctcaagtag gcttaaccca taaattaccg gtcatctttg
     2461 gtatcttaac cgttgaaaac atcgaacaag ccattgaacg ctctggtact aaagctggta
     2521 accttgggtt tacttatgca acgaatacta ttgaaatgat taatttattc aaagaaatta
     2581 aacaacaata agtcatcatt ctatatgaaa atatctgtaa cagcataggg ctagctttaa
     2641 ttttcaatca tggtaagatg gaagatagga aaataaagcg cattcaatta attatcaagc
     2701 ctttctgatg atttgcgcaa aaagcttaag gagacttcat atgacgattc aatttaaaca
     2761 aacattagcg ccaaccaagg ataattctgg aaaaccaatc attttaagtg acgctacctt
     2821 cgctcaacgt aatgagaaag tccttcagtt aatgcgtgaa tatgacttct catcattgat
     2881 gatttatgca gataaagaac acggtagtaa ctttgaatat ttaactggtt ttattccacg
     2941 gtttgaagaa gctatccaag taatcaacca agacggaacg tcgacactga ttttaggtaa
     3001 tgagaattac aataaaacca gtatggcacg tacacaaagt gaaggtattc tttgcccatt
     3061 cttctcttta ccaaaccaac ctatggattt cactaaaact tttgctgact atcttaaaga
     3121 cgttaaagta gatgcttcaa aacgaattgg tttagtagac tggaagcttt tatctaatga
     3181 tatggacaac ttccacaacc atcattcaat accagcattt attgtagatg gtatacatga
     3241 agttttcggt tctgacaagg tagtgaatgc aacccaacta tacatgcatc caaaagaagg
     3301 tgctcgtgtg acaaacaacg cagaggaaat cgctcgttat gaatatggtg cttcccttgc
     3361 ttctgatgct gttcttgaag caatgaacca gctaaccgtt ggccaaagtg agttagcagc
     3421 aggtaatcac ttaaatcgcg atggtcaata tccatctgtt gtgaccattg cagcctttgg
     3481 tgatcgcttt gagggtgcta atatttatcc aactgataat acgctaaatg atggcgataa
     3541 agttgcatta actgtagcct ataaaggagg cttgtctagc cgctctggct atgcagtaaa
     3601 agaggaaagt cagctcgctg aacatgatcc aggatactta gaagaagtgg tttacccata
     3661 tttccaagcc tataaatggt ggctagataa tttaaccatt gggagaaagg gtggcgattt
     3721 ctaccaagca ttcagtgact tctatccgca agctacttat ggctggaatt tgtgcccagg
     3781 gcatttagta gctgacgaag aatggttaag ctcaccattt tatcaagact ctgatgcaac
     3841 aattcaaagt ggctacatct tccaactaga ctttattcct tcacaagcag gccacaatgg
     3901 tgtttcagct gaatcaacga ttgcggttgc ggacgcggca ctacgtgaac agattaaggc
     3961 tgaacaacct gaattatggg cacgtatctg taaccgaaag gattatctaa aagaagcatt
     4021 aggtttagaa ttatcagaac atttactacc cttagcgagt acctttgcct actatagacc
     4081 tttcttatta gacaaaaaaa ctgctatcta ctttggataa aaaaatgccc ctcaagattg
     4141 tttgccaaac ttgagggttt tttgtctatt tttaagtgaa taatgacgga ctatccaaac
     4201 ggaatcgcca taaagatgtt aatatatgta taatattata aagaaattgg aggacaatgc
     4261 ccttggacga gcagattgaa ttattattta ccatagatga aggctatttg aatcccttaa
     4321 aagtggcttt aacatcgatt cgtcagaata atccaggcca agctttccgc atttggttga
     4381 ttcacgaatc cattgcaatg catacgattc gcgaattgca gaagctaacg gattatctac
     4441 agtttggttt tgaagcgatt aaaattgatg gtagtcgatg gaatagcgct aaaactgaag
     4501 atagataccc aaaagagatg tatttccgcc tgcttgcggg tgaaatatta cctaaagaaa
     4561 tgaaacgcgt gatctattta gacccagata ttttagtggt taacccttta ttagatttgt
     4621 ggcaaacaga tttagaaggt tatatgctag cagctgctac acatgttgga ttgacggacg
     4681 tttctacacg agtaaaccaa atgcgacttg acgttgacca tgcttattac aattctggtg
     4741 tgatggtgat tgatttagat aaagctagag aaattattaa atggtcagat attgcacaaa
     4801 tgattgaaaa atataacttg ctattaatcc tacccgacca ggatatcctc aaccgattat
     4861 atggtaaata cactaaggaa attccagaag aaatttggaa ttatgatacg cgtaaatata
     4921 tgcggtattt caccaaaagt ctcgctcaac atgatattca ctgggtgatg gcgcatactg
     4981 ctattctcca tttctgtggt ggcccaaaac catgggacga taaacatgat aatcgtttta
     5041 ccagtcttta tcttacctat caaaatcaat taaagcgtat tgaagatttg atgaagtaaa
     5101 gtgaaacaag tgtttttcat tacctacggt ggtttaatgc ttatttatta tcgttaaatc
     5161 atgcttatac act
//
LOCUS       DAFFFQ010000122         4891 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000122
VERSION     DAFFFQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.127"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..561)
                     /locus_tag="LQB69_002765"
     CDS             complement(<1..561)
                     /locus_tag="LQB69_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL3526871.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="LQB69_002766"
     CDS             complement(748..1398)
                     /locus_tag="LQB69_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526872.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LQB69_002767"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="LQB69_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL3526873.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="LQB69_002768"
     CDS             complement(2910..3908)
                     /locus_tag="LQB69_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL3526874.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LQB69_002769"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="LQB69_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL3526875.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
BASE COUNT         1486 a         1050 c          757 g         1598 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata t
//
LOCUS       DAFFFQ010000123         4878 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000123
VERSION     DAFFFQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.128"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(207..1169)
                     /locus_tag="LQB69_002770"
     CDS             complement(207..1169)
                     /locus_tag="LQB69_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3526876.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="LQB69_002771"
     CDS             complement(1171..2610)
                     /locus_tag="LQB69_002771"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL3526877.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKTAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="LQB69_002772"
     CDS             2795..4741
                     /locus_tag="LQB69_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL3526878.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1566 a          826 c          794 g         1692 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcagtt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatg
//
LOCUS       DAFFFQ010000124         4824 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000124
VERSION     DAFFFQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.129"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1227
                     /locus_tag="LQB69_002773"
     CDS             <1..1227
                     /locus_tag="LQB69_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL3526879.1"
                     /translation="NQDSSAQQEEMKKIIEEYNQYFDTAWSLADIERYNGDINNCLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MKSIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIG
                     VYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDARD
                     EIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDELIHAFSN
                     EWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPKAKPFKYPQEMKRAW
                     RKMLDDELIPLENELR"
     gene            1342..3114
                     /locus_tag="LQB69_002774"
     CDS             1342..3114
                     /locus_tag="LQB69_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3526880.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            3089..4381
                     /locus_tag="LQB69_002775"
     CDS             3089..4381
                     /locus_tag="LQB69_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HBL3526881.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         1820 a          714 c          911 g         1379 t
ORIGIN      
        1 aaccaagatt caagtgccca acaagaagaa atgaaaaaaa ttattgagga atataatcag
       61 tattttgata ctgcctggtc attagcagac attgagcgtt ataacggaga catcaataat
      121 tgtttggcac gaaaaagagc ggaattcaaa caatttggga aacaaatcga tttagtaatc
      181 gtagtggatc gtctattgac aggttttgac gcacctacga ttcaaaccct atttgtggat
      241 cgtaatctag agtatgcagg attgattcaa gctttttcac gtacgaaccg tacgtatcct
      301 gaaaagacaa aaggattgat tgtgacgttt cgtaagccag cgacaatgga gaagaatgtc
      361 gaggatgcga caaaactgta ttcagaagca aaagaagaat cgggtttgat ttatccaagt
      421 tatcaggaat ccaaaaaaag attcaagcaa gcgtatggta agttaaagga ggtttcatcc
      481 atgaaatcca ttgatgaaca tacgccactt gaaatccgta tcgagtacgt taaagcattt
      541 caagaattga atcgtgcgta tgaatcgtta gtgacttatg acgattacaa tgatgacatg
      601 gtgcagtctt caactttgaa caatcaaatt caacttttgg aagaacaaat tggcgtgtac
      661 gagaccgtga aggggtcatt aatagaggaa gaaccagaaa aagagggaga agatttttca
      721 acgatcgaat tttatagtga aaataccact aagttgtatg atattgattc tacttatatt
      781 gatcaattac taggaactta tgctgcgaat agttcagatg cacgagatga aattgaaaaa
      841 gctttggcta aattgaataa aacagaagga gtcaaagaag tttaccgaca aattttaaat
      901 gcatttgatg tggggacttt agatagcaat gaagatattt ttgtcattaa acgtcgctat
      961 tttacacaag caagtgatga attgattcat gccttttcca atgagtggtt tgtttctgag
     1021 aaagaattac atgcttctaa tctgcaatat atgccaggtg aagatccaat tcctaatatg
     1081 aaagcaatta tcaacagtaa ggattacgaa ggatataaag ccaaacatcc aaaagcgaag
     1141 ccatttaaat acccacaaga aatgaagcga gcctggcgta aaatgttaga tgatgagttg
     1201 attccacttg agaatgaatt gaggtaaacg agaaaattga agaaattgta tgataaacat
     1261 gttaataaat gatgctcgaa ataaaatttg gttttatttt tacattgata cggtaaataa
     1321 aatttgaaag tagaagaaaa tatgaacaac aatgaaacaa aaaaaacata tgaactaaga
     1381 ataactgctg atgagattaa atcaacagac ccaacattta ctatgaagaa tctaagctta
     1441 ggggataatt atcaaccaaa agatgcttac ttccgtacgg cagaaggcga aataactaga
     1501 agaaactacg gtaaaaatga acggaaaatt tcaaatcaaa cattaccgcg attagctatt
     1561 caaatgtttg aacaatcgat tgtttctcta ccagaaagcg aaagagcaac atttcctgtg
     1621 tgtcgatatc actcaggaga tgagataata cgagggattt ttccagatgt tgacagtttc
     1681 aaaaaaaaaa ggaagtccat tgaatttttg atttataaat ataatgaaga caagcagata
     1741 gtcatctatt gttggagtat tttttcaact ttattatttg tacaagaatg tttgaaacgt
     1801 tttggaacat ctggggatga atttattctt gtttataaag ggaaagagaa aaaggaaata
     1861 cgagtaggag agactcagac tatgaccaat catttgctga aatcatatgt tttacattta
     1921 ctcaaatcta aaaaccttat tctacgtggc gctcctggaa cagggaaaac attccttgca
     1981 aatgaaatgg cagctgatgt cgtttcaaat ggcagaacta cacaaatcaa tgaattgagc
     2041 gaagatgaaa agagccgaat tggatttgtc cagtttcatc ctagctatga ttatactgat
     2101 tttgtcgaag gtttacgtcc aactacatcc gatgatggcg ttgttagttt caccttgaaa
     2161 gatggaagct ttaagagctt tgttgaaaaa gcaaagaaaa caaagaaagt aaatggacaa
     2221 gataattttg aagaagcttg ggctaaattt ttcgatgcgg taacggaagc tagtatagag
     2281 ggagaaggct acaataaact taaaacattg acaggtaaac cagtacgcaa tcttctagcc
     2341 tatgaaagaa atgaaattca aggtgtttat cctgctaaaa ccaatactta tctaaaccat
     2401 gaccaaattt ataatgtcta tcgtggattg ccaggtacgc caaaaggtgg atttgatgct
     2461 tatcgaaaag cgattgtaaa acatttaaaa gaagaatttg gcttaaaaaa ctacacggcg
     2521 tcaacggtag attctacgaa taaccaaaac tatgtgttta tcattgatga aattaatcgg
     2581 ggcgaaattt ccaaaatttt tggcgaatta tttttctcaa ttgatccagg atatcgtggg
     2641 cataaagaag gtgtttacac tcagtatgca aacctccatg aaaatccaga agaaaaattc
     2701 tatattccta ataatgttta tattattggg acgatgaacg acattgaccg ttctgttgac
     2761 acttttgatt ttgctatgcg ccggcgattt actttcttag aaataaaagc agaagattca
     2821 gcacaaaata tgctgactca agagaaaaca aaagaactta tgacgcgttt aaatgaggcg
     2881 atcgttgcca aagaaaaagg tggattgtca actgattacc aaattggtgc tagttacttt
     2941 ttaagtatcg ataatggtga ggagacaatg gaacaattat gggatactaa gttatatcca
     3001 ttattgaaag actattttca tggtgaacat aaggcgaaag aaaaattaga aatactagaa
     3061 aaggtatact ctaatgacgg tgatttagat gaagctgaaa cctctacacg ataatcaaga
     3121 aatcaatgac tcagttgtgc aacatccaat cattacgaac ttagttaatc gtgacttaag
     3181 tagtttagag aaagaaaatt ttattatctt tccgcaacag ttaaatcaat ccaaggatct
     3241 aagtgaagat aacttgatat ttcaatcaag aaatggaaaa atttggactt gtaatgtcgt
     3301 aggtatcctt agcgattcaa cagatgaatt aagaattcat tctagattcg ctaatcaaat
     3361 gaagcatgaa gatttctttt tacgttatat gcttcagcgt gttttgaatt acaacgtgat
     3421 taatgatcat tttagtgcat ctaaaaagat gacatactac gatttgttgg tatttctctt
     3481 tccttattat ctgaatgaag caatgcgtaa agggacatac aaagaatatg tcaaacgaga
     3541 atacaataat gccaatatca agggagctgt tgatgttgca aaacacatcc gttcgaatgt
     3601 tcctttcgtt ggaaaagttg cttatcgaac acgagagttt agctataata atcacctaac
     3661 ccagcttatt cgtcatacta ttgaaaaaat ccaaaatgag tacgatttct tacttagtgg
     3721 agatgaggat acgaaagaaa acgtggttct aatcaaacaa acaacacctg gttatgctag
     3781 attaaatcaa tttaatatcc ttcaagagaa tatccttcat ccagtcaaac atagttatta
     3841 cgaggagtat tcggctcttc aacaaatctg tatccaaatt ttatcggaag aaaaatcagg
     3901 atttggaagt gataaaaatc aaatacatgg aatcattatc gatgtgtctt ggctatggga
     3961 agaatacatt ggaaaagtca ctggttggaa acattatggc agagataaag ggttagcaac
     4021 aatgcattta tttcaagagc ccaaccggtc cccacgatat ccagatttta catttaataa
     4081 tataccaatt gatacaaaat acaaaaaaca tcttgatact agaaatgact ataatcagtt
     4141 agtcacatat attcatataa tgaatttaga ccaagcagat acaatgaaag gcggctttct
     4201 gcaacctact tccgatcccc ttcctgcaaa tcatgggtat aaaaaaatag gcatattaag
     4261 cgggctagga ggagaaatat tcacatatca ttttttcatt ccgcaagctg cgtcaagtta
     4321 tttggaattt gtagaacaga tgagagaggt agaagcagca ctacaaacta cttttcaata
     4381 agtaggaatt atagaaattt tactcaaaaa atcttcatta aaaaatctga ctaaataagt
     4441 tgagaaaaca gaatatactt caatatccag tcatttataa atatcattca ctcatgatac
     4501 ccagctccgt atcacaaata atgatatttt ttaagagaaa aaattatccc tattttaaat
     4561 aatagcaata ggtagtggta gagagaaatt tataaataac tttgttattt gctttggtag
     4621 ccaagtaagt tgcaaaagaa aaatagatac tgttaaaaag ataatgtaga catagaaaca
     4681 aatacgtaaa attttagtta gaatgatggc aaggagaaaa aacatgatag atgaaaaaaa
     4741 gtgtttaaaa aatctattgg aagatatgga tgatattgaa gaaaaactat ctaaatggac
     4801 tgggaaaaca aatatatttc agaa
//
LOCUS       DAFFFQ010000125         4810 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000125
VERSION     DAFFFQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.130"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="LQB69_002776"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="LQB69_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL3526882.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3253..3771)
                     /locus_tag="LQB69_002777"
     CDS             complement(3253..3771)
                     /locus_tag="LQB69_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL3526883.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1581 a          786 c          730 g         1713 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta
//
LOCUS       DAFFFQ010000126         4783 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000126
VERSION     DAFFFQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.131"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            544..1107
                     /locus_tag="LQB69_002778"
     CDS             544..1107
                     /locus_tag="LQB69_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3526884.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="LQB69_002779"
     CDS             complement(1386..1673)
                     /locus_tag="LQB69_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3526885.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="LQB69_002780"
     CDS             complement(1663..1992)
                     /locus_tag="LQB69_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3526886.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="LQB69_002781"
     CDS             2297..2626
                     /locus_tag="LQB69_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526887.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LQB69_002782"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="LQB69_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL3526888.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="LQB69_002783"
     CDS             complement(3551..4462)
                     /locus_tag="LQB69_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL3526889.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1564 a          824 c          722 g         1673 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tct
//
LOCUS       DAFFFQ010000127         4680 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000127
VERSION     DAFFFQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.132"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(92..955)
                     /locus_tag="LQB69_002784"
     CDS             complement(92..955)
                     /locus_tag="LQB69_002784"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL3526890.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(1097..3670)
                     /gene="mprF"
                     /locus_tag="LQB69_002785"
     CDS             complement(1097..3670)
                     /gene="mprF"
                     /locus_tag="LQB69_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL3526891.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3811..4617
                     /gene="proC"
                     /locus_tag="LQB69_002786"
     CDS             3811..4617
                     /gene="proC"
                     /locus_tag="LQB69_002786"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL3526892.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         1462 a         1026 c          809 g         1383 t
ORIGIN      
        1 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
       61 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
      121 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
      181 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
      241 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
      301 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
      361 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
      421 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
      481 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
      541 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
      601 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
      661 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
      721 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
      781 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
      841 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
      901 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
      961 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
     1021 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
     1081 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
     1141 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
     1201 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
     1261 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
     1321 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
     1381 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
     1441 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
     1501 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
     1561 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
     1621 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
     1681 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
     1741 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
     1801 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
     1861 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
     1921 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
     1981 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
     2041 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
     2101 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
     2161 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
     2221 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
     2281 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
     2341 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
     2401 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
     2461 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
     2521 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
     2581 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
     2641 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
     2701 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
     2761 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
     2821 ctactgctac atcttgccga acagacaatt gactcattcc taagatcatc aaaacgtcaa
     2881 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
     2941 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
     3001 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
     3061 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
     3121 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
     3181 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
     3241 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
     3301 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
     3361 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
     3421 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
     3481 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
     3541 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
     3601 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
     3661 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
     3721 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
     3781 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
     3841 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
     3901 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
     3961 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
     4021 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
     4081 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
     4141 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
     4201 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
     4261 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
     4321 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
     4381 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
     4441 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
     4501 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
     4561 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
     4621 tagcaatgtt ttttcgtacc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
//
LOCUS       DAFFFQ010000128         4661 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000128
VERSION     DAFFFQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.133"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(172..474)
                     /locus_tag="LQB69_002787"
     CDS             complement(172..474)
                     /locus_tag="LQB69_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526893.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LQB69_002788"
     tmRNA           complement(694..1060)
                     /gene="ssrA"
                     /locus_tag="LQB69_002788"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1231..2004)
                     /locus_tag="LQB69_002789"
     CDS             complement(1231..2004)
                     /locus_tag="LQB69_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL3526894.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2148..2498
                     /locus_tag="LQB69_002790"
     CDS             2148..2498
                     /locus_tag="LQB69_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL3526895.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2479..3195
                     /locus_tag="LQB69_002791"
     CDS             2479..3195
                     /locus_tag="LQB69_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526896.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3195..4643
                     /locus_tag="LQB69_002792"
     CDS             3195..4643
                     /locus_tag="LQB69_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3526897.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
BASE COUNT         1550 a          821 c          900 g         1390 t
ORIGIN      
        1 ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca ctgatactat
       61 aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat atccctttta
      121 taagtatggt gataaaatcc gttccgggct acctttttta ggtagcctac tttaatcttt
      181 atacctttct ggatcaacga aagtatactt caaatagtca taacgccgat gatcgctccg
      241 tgcgtctggc acatcagtca cgatatcaaa caaaaaatac acatctttct tcattctggt
      301 ttttgttgcc ggaattttaa agtagttctt attagagtaa tagagattga ttaataagct
      361 atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat ctttgacaaa
      421 tctattcgaa ggatcaaatt taaaccataa ttgtgttttt ccttccatca acataatgtt
      481 cacctcaaaa agagtatacg aactaatgtt ctttttgtaa agtaggaata tcattgataa
      541 tatggatgga acatatatat taaaaagtgt tctttttgaa tgctattttg aatgccattt
      601 tcattcattt aacgaaaatc tgcgaaaata aaaaatgctc aaacccttat aaaataagga
      661 tttaagcatc tagcgaaatc tgctgaaatc tcacaatgga gacggcggga gtcgaacccg
      721 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      781 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      841 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      901 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      961 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
     1021 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
     1081 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
     1141 tcatatcgca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
     1201 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
     1261 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
     1321 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
     1381 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1441 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1501 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1561 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1621 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1681 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1741 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1801 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1861 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1921 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1981 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     2041 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     2101 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     2161 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     2221 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     2281 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     2341 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     2401 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2461 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2521 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2581 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2641 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2701 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2761 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2821 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagt agtttcagat
     2881 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2941 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     3001 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     3061 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     3121 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     3181 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttctgc ggaatggtcg
     3241 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     3301 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     3361 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3421 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3481 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3541 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3601 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3661 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3721 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3781 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3841 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3901 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3961 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     4021 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     4081 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     4141 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     4201 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     4261 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     4321 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     4381 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4441 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4501 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4561 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4621 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa a
//
LOCUS       DAFFFQ010000129         4651 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000129
VERSION     DAFFFQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.134"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            1..367
                     /locus_tag="LQB69_002793"
                     /pseudo
     CDS             1..367
                     /locus_tag="LQB69_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            383..532
                     /locus_tag="LQB69_002794"
     CDS             383..532
                     /locus_tag="LQB69_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526898.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            613..1209
                     /locus_tag="LQB69_002795"
     CDS             613..1209
                     /locus_tag="LQB69_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL3526899.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1222..2121
                     /locus_tag="LQB69_002796"
     CDS             1222..2121
                     /locus_tag="LQB69_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL3526900.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2124..2255
                     /gene="ssb"
                     /locus_tag="LQB69_002797"
     CDS             2124..2255
                     /gene="ssb"
                     /locus_tag="LQB69_002797"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3526901.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2277..2609
                     /gene="ssb"
                     /locus_tag="LQB69_002798"
     CDS             2277..2609
                     /gene="ssb"
                     /locus_tag="LQB69_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3526902.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2654..2887
                     /locus_tag="LQB69_002799"
     CDS             2654..2887
                     /locus_tag="LQB69_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526903.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3046..3168)
                     /locus_tag="LQB69_002800"
     CDS             complement(3046..3168)
                     /locus_tag="LQB69_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL3526904.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3358..3582
                     /locus_tag="LQB69_002801"
     CDS             3358..3582
                     /locus_tag="LQB69_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526905.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            4025..4231
                     /locus_tag="LQB69_002802"
     CDS             4025..4231
                     /locus_tag="LQB69_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL3526906.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4231..4482
                     /locus_tag="LQB69_002803"
     CDS             4231..4482
                     /locus_tag="LQB69_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526907.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            4498..>4651
                     /locus_tag="LQB69_002804"
     CDS             4498..>4651
                     /locus_tag="LQB69_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526908.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
BASE COUNT         1670 a          802 c          942 g         1237 t
ORIGIN      
        1 ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt tgtctctcac
       61 ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac gttacaagaa
      121 gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc
      181 caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga agcctgtgat
      241 tttaaaaggt cagaagggtt catttgaagc aacgattgtt ttaaaagaag acttcactac
      301 aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac gccgcactgc
      361 aaaataatgg agggttaatc ttatgcgtaa taccacacac aaacaacaag aaatcgaaaa
      421 tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac gaagactgca
      481 gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa ttagatcgat aaaatcttcc
      541 taactattgt atacgtataa acttatgtta tactgttttt ataagtttat acgtatacaa
      601 aggagggaga aaatgacaaa agcaatggta tacaagctgc tagaagaatt taatggaact
      661 ttggctttga aggatgccaa aaaagcgggc atttctcctg taacgatcaa acgtatggta
      721 gatcgagggg agctggatcg agagtatcca ggttttttca ccttacctgg tcaatttcct
      781 gatgaattgt ttatggcgca aaaaaaatat gaacgcggca ttatttcaca tattaccgcg
      841 ttagatttgt atgatttaac agatatgatc cctagacaga tcgatctgac tgtaccttac
      901 ggttatcacg tttctgaaaa aggattgaag gaattcgcag ttgagctaca ctatacaaaa
      961 gcggaatggt atgaattagg taagattgaa ataaaaagtc gctatgggaa tcctattata
     1021 gcttatgatc cagaaaaaac cttgtgtgac atttggaatc cctggtataa cgttgaggat
     1081 gaaatcaaag taaaagcaat caaaaactac atggagtcgg acagaaaaaa tcttagaaaa
     1141 ttgaatgagt atcgacgaat tttgcctaca gataaaacga tgcgctctta tattatggct
     1201 ttaaattaaa ggagaataca catgacacct acgcaattaa aagcaaaagt gaagaatatt
     1261 tctagagaaa aagaagttga tccgcagctt gttttaagac attttatgat ggaaaaattc
     1321 ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg tgttaaaagg cggcttctta
     1381 attggctcaa agtatggcat tgaaaatcga acaactaaag acattgatac gactttacgg
     1441 gaaatgaaag taaccaaaga aacattgaca acggttctaa acgatatttt ttcaacgccg
     1501 acgaaagaag gcattcaatt tgagattcaa ggaatgaaag aaacgaggga agctgattat
     1561 tatccagggt ttagcttacg tgtactggca catttggaaa acatgcggcc agattttaag
     1621 gtagatgtta ctacaggtga ctcgatctat cctgcgacga tcactcacag tcataaattg
     1681 atgtttgagg atcgaacgat tcctttggaa tcctatccta cagaacaaat tattgctgaa
     1741 aaattaagtg caacctttga ttttttaaca gataattcga gaggaaaaga tttttatgat
     1801 ctctatacta ttcctaaaat ggagaagatc gatgagaaga ccttgaaaga tagtgtgaga
     1861 aatacattta cacgtcgagg aaaaactgat ccgttaaaga tatactatga aaaggatatg
     1921 gatgtactga aaaatagtca cgagctaaaa gatacctgga agaaatatca aaaaagcaat
     1981 ccttatgctg cgtcgatcac gtacgatgag acaatggatt cgattagtaa tttgatggaa
     2041 aaagtgattc acgtcgaaga aaaagagcga aaactagctt atcaaagaca ccaacagatg
     2101 aatagagaaa tggagagata aagatgatta ataatgtgac attagtagga cgcttaacca
     2161 aagatcctga tttacgttat accgcaagtg gtactgcagt agcgactttt accctagccg
     2221 tcaaccgaaa tttcacgaat caaaacggca attgagaagc cgattttatt aactgcgtga
     2281 tttggcgcaa accagcagaa acaatggcga cattagctaa aaaaggtagc ttgattggtg
     2341 tagttgggcg gattcaaacg cggacttacg acaaccagca aggacaacgt gtatatgtga
     2401 ctgaagttgt agcagataac tttcaactat tagaaagtaa agcagcgaca gagagccgtg
     2461 cgcacgctga tcaatcgtcc accagtccaa gcaccgccac ctttgaacaa cggaatacag
     2521 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
     2581 atattagcga tgatgatcta ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa
     2641 aattagtctg acgatggatc tattgatacc cgtaattgca tttatagacg ggatttcttt
     2701 gggcgtcact gaaaatcctt tggtaaatag aattgcgaaa ggatcactat ttcttgtatt
     2761 cgtgtatctt gtctgttctt atcttttaaa aagagcagga aaggataatt ccttttggcg
     2821 attgactaaa gtcacagtag cagcgacagc tgttatactt acggctattc taccgcttta
     2881 tatgtagagt taacgggaaa tcgttgaact tcttgcgaaa tattgctata atgaacatga
     2941 aaaagaggag ttgcgctaac aacccctcat taacagcacc gattaaagaa cggtggcaga
     3001 atattagtta tttggaaata accgctgacc tgctaaagta gacggttatt tctttttgtc
     3061 atttttaagc atatttacaa tcaaactaat caaagcaatg gtaaacatac caaaaccaag
     3121 aatggtttga attgtctcat atgcggacaa aaggcttact cctttcatag attttagcac
     3181 ttacattcat aagcaccacc acctttccag aagtcgccac cgtcttcaaa ctttactgtc
     3241 ttgactatta tagcatatct aaaatcgaat aaaaattctc aattttagct gtttttgcta
     3301 taattttagc gaggtgatcg tgatggaaca atatattggt ttactcgata aattagggtg
     3361 atcaagacac agccattgct tgtgcgattt acattacgca cgagtcatga accaatcaat
     3421 tgtgtagtgg ccaatataga aatagcggat aaattgctga ttttggaaga aggcaagtat
     3481 actatttcag tgacaggtca cttcaataaa cgaaagcaac tagtcattga atcaatgcaa
     3541 attagaaacc ctgatcattt tacgcgatcg atgagcatat aaaagaagag ctgcagtcct
     3601 aaatgaggat tgtagctctt tttgttttac atagaaacag taaaaatgaa aaggcctgtt
     3661 taggaaggca caagcgcctt ctcaattgaa agacctttct ctcactccaa ccgccaccgc
     3721 cgttcgagaa gaaaggaagt gaacgctacg cgttcactaa gataaagggt cttgcatagc
     3781 aagacaaaat agaaaaaatt tcctctcacc aaaaggttcg ctcctgttcg ttatctcttt
     3841 attgatttgt cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg
     3901 gtaaatggca cgctgcgcgc ccttgtcttg tcgcttttcg ttgattcatt ctggccaaca
     3961 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     4021 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     4081 tcatgcacca catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta
     4141 ttgtttgtat caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattatat
     4201 tttaattgat gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta
     4261 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagtt
     4321 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
     4381 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacttggaa
     4441 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
     4501 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
     4561 gaggatatga cgttcctata caacgaaaca cgtgttccaa aaaaacatta taaagaaaaa
     4621 ttgtcggtag ctgtggaaga aatgattgaa t
//
LOCUS       DAFFFQ010000130         4617 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000130
VERSION     DAFFFQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.135"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(14..961)
                     /locus_tag="LQB69_002805"
     CDS             complement(14..961)
                     /locus_tag="LQB69_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL3526909.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1139..1942)
                     /locus_tag="LQB69_002806"
     CDS             complement(1139..1942)
                     /locus_tag="LQB69_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL3526910.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2088..3002
                     /locus_tag="LQB69_002807"
     CDS             2088..3002
                     /locus_tag="LQB69_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL3526911.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3050..3520
                     /locus_tag="LQB69_002808"
     CDS             3050..3520
                     /locus_tag="LQB69_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL3526912.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3605..4213
                     /locus_tag="LQB69_002809"
     CDS             3605..4213
                     /locus_tag="LQB69_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL3526913.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4347..4547
                     /locus_tag="LQB69_002810"
     CDS             4347..4547
                     /locus_tag="LQB69_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3526914.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         1511 a          716 c          912 g         1478 t
ORIGIN      
        1 ttgttttcag ctattaatat cctgcagaca attttacttg attggtttga agctcatggg
       61 tggcaaagta gcttttcaga ttggttaaaa aaatagccat taatttcttt tgaaaatcag
      121 gtgtcaaacc tgcaatgtgg gaagtaatca gtacattgtc cattttccaa agaggatgat
      181 cttgtggaag aggctcttcc tcaaaaacgt ctaatgctgc aaaggaaagt tgctggttat
      241 ttaaagcctg caccagatct tgtgtcttaa cagaagcacc acggccaaca ttgatgaata
      301 cagattttgg atcaaagtgt tcaaacatat gctgatcaaa gagcccagtt gtttcctccg
      361 ttagaggtaa gatattgact acgaaatcta cttcaggaag aacttccttt agttgattta
      421 aagaaaaagt cttttccatt ccttctgctg gatgaccgga tgtattgatt ccatagcagt
      481 tcatgtctaa tgctttgcat ttttttgcaa tcatttttcc aatatggcca gttccaatga
      541 ttagtatttt tttcccttgg atagattggt aatggaccga tgaacctaac catttcatgt
      601 cactctgtgc cttttgtgct tgaaaaagac cacgagaata cccaaggatg attcctaaga
      661 catgctccgt aatcgattca ctgtggatgc cactaccatt tgaaagtaaa atatgtttct
      721 ttgaaaaagt ctcaaggggt agctgatcaa caccagctga gatagattgg acccatttca
      781 aggtagaaga ggctaatagt ttttcttggt agtctgagtt ccagcccaaa ctgatctcta
      841 cattttgtag atcctctggt tgtaattctg ttttaaagat ataaccaggc gctatagtat
      901 ggatttcttc gataaattca tcacgaaatg gacgatctgc ataaatgatt ctttctgaca
      961 tagttaattt cccgcctttc tttactctaa gtgtagcgca tacaagaaga actttgaagc
     1021 gccttgcttg ttttttgaga aagaaatatt tttcaaataa aaaaggggct gtgacaaaaa
     1081 aatgtcacaa cctctaaaag ggtctattga agtagaaata acgagttgtc tttatttctt
     1141 aattgatcga tatttttgcc catcctttga cagcttgagc aaaaataaag attcctgcaa
     1201 aagcaatgaa ggcgctagtg tcaaaagaag gaagaaagac catcaagatt gttgcaatta
     1261 tagtaggcaa tgaaattgaa tagatcaatg tttttaaaca atcaagaaaa gtagcttttg
     1321 ttagtcgaaa gcgtgcatag agaaaagcag caatgttggt aaatagcaaa gtgatcagca
     1381 gattcagaaa actaggataa atagaaaata taaatgtcag gacttttatc caaagtggta
     1441 tagatgcttc acttaaaaaa gaacggagct gcttacctgt taagtttttt aaagattcat
     1501 tggtgtaggg aagttctaat tggttattgt tcaacaaagc agaagtcgtg cctgtattag
     1561 gaagagctat gataagtttg ttttttaaca tcccgacact aacaaaattc cccatcaaat
     1621 cagtagagat gtcctgttct gttcgttttc cctctggatc aaatgtgaag ataatggagt
     1681 ttgtttggta aatgaatcct tctttttctt cggtttgaag ctgcccgttt tggattgtaa
     1741 aatcaggaat gcgagtagca atcttttgcc catccaattg aatgttgttt aagacttgaa
     1801 aaacttgaaa agagattggc aaagccataa ttatactcaa aactaaaaga tagacaatgg
     1861 ctttccaaaa tgggacattt ctcgcctcct ttaggtcttt gaatcgtata aaggaactga
     1921 taattaattg ttttgttgtc ataagtactc ctcatctagt agtgtcatca cttgcaattc
     1981 tagcgaagta tcaagtgaaa tacaagtgct ttaaggaaac agctgaatga ttggtgaaat
     2041 atcaaaaaaa tgttattctt gtctaaagac tatccaatat aaataaaatg gaggaaggaa
     2101 tagtgcctac acagccacag agatcatcaa aaaaactatt tggagtagtg gttttgaatg
     2161 cggtgatcac tttatcagaa tttattggag ggattatgtc agggagtttg tctcttgttt
     2221 cagatgcttt ccataacctt agtgatacat tggcaattat tttttcttac ggggcacaaa
     2281 aaatggcccg aaaagaagca aataaaaaac gtacgtatgg ctaccagcgt ttagaaatat
     2341 tgagtgcttt catcaactca ttcatattga tcatattatc cttgttttta accgttgaag
     2401 cattcaagcg gttcacttca ccagaaaaga taaatagtca tttgatgcta acagtagcag
     2461 ttattggatt gctcgcgaat ttgttctcag tattattatt acgacaagaa gcagatgaaa
     2521 gtttgaatat aaaatctagt tatttgcatt tgttaagtga tacactgtct tcggtaagtg
     2581 tgattatcgg agcaattttg atccgttttt tcgatatcta ctggatcgat ccagttatta
     2641 cactgatcat ttcgatatat atattgatag aagcgataat ggtgatcaaa aaagctgccg
     2701 ctattttgat acaaagtgca cctgctatcg actatgaaaa aatggaacaa gaaattgaag
     2761 caatcgatgg ggtaaaggat gttcatcatg tccatatttg gcagtatagc gaaaagataa
     2821 ttattttcga cggacatatt gattttgaag atcagctact aagcgagatt gaaaagatat
     2881 atcctcgtat aatttcttta ttaaaattaa aatatggtat tacacatgtg acgatccaag
     2941 cagagacaaa ggcaaaggat caaaaaaaat taattttttt aaataaagat gaaagttatt
     3001 gaaatataga gtatgataaa taagaatttt taataaggta ggttggctca tgaagcagta
     3061 tcgacagtta aaaggatatt tagaagcaaa aggatattgg gagatactag tctatctctt
     3121 ttttggtggt ttggctacga ttgtaaattt tgttagtttt gctctcgcac gccaatattt
     3181 tgatttaagt atggccttat ccaattctat ttcgtggttt tgttctgtat tgtttgcttt
     3241 tgtaacgaac aaactttggg tattccattc aaaatcaccg aattttacac atgcacttat
     3301 tgaatgcggt aagttcttct tctaccgtat cctttcttac ggattggaca tgggggcaat
     3361 ggtattgttg atcaatgtta tgaacagcaa tgagtatgtg gcgaaaatca tcactcaaat
     3421 tattgttatc cttgcgaact atattttcag caagttgttt atttttaaag aaactgaggt
     3481 ttttgaagaa gaagtaggtc aaagtgaaaa agacgactag gcataacaat tgaagaattt
     3541 ccttctcttt gttactcttt gtaatgtaga cattattaag aaaacattga ggaggaattt
     3601 ttttatgact tacacattac cagatctacc ttatgcatat gacgcattgg aaccttatat
     3661 tgacgaagaa acgatgcatc tgcatcatga taagcatcac aatacttatg tgacgaattt
     3721 aaattcagca attgagaaat acccagaatt aggcgaaaaa acaatagaag aattattatc
     3781 tgatatggac gctattccaa cagatatcaa gacagctgta cgtaacaatg gtggcggaca
     3841 tgctaaccat tcatttttct gggaaatcat ggcaccaaat gctggtggcg aacctacagg
     3901 agaaataaaa gaagcgatta atgaagcttt tggtgatttt tcttctttta aagaagaatt
     3961 caaaaaagca gccgctggac gatttggttc tggatgggct tggcttgtaa tggaaaatgg
     4021 aaaattagct attacctcta ctgcaaatca agattctcca ttgatggaag gaaagacacc
     4081 aattctaggt ttggacgttt gggaacatgc ttattatttg aaatataaaa atgtccgacc
     4141 agattatatt gcagcattct ggaatgttat taattgggat gaagtaaaca aacgtttcga
     4201 agcagcaaaa taaccatttc ctccgtcata tcagtcttaa aaataatttg gaaaataaaa
     4261 gagcgaaagc ttgctagcgt tttctaaatt gggtataatg ttcttgcaag gttacttgca
     4321 agaacttttt tggaggatat acatttatgg aacacggaac ggttaaatgg ttcaacaatg
     4381 aaaaaggatt tgggtttatt acagtagacg gcggagatga cgtatttgtt catttctcag
     4441 caattcaagg agacggcttc aagagcctag aagaaggtca agaagtagaa ttcacgattg
     4501 ttgaaggtgc acgcggacct caagctgctg aagtgactaa attataataa tttgttgaat
     4561 aaagacacga cttcttagag agtcgtgtct ttttgtctat ttatgaagtt tttagat
//
LOCUS       DAFFFQ010000131         4556 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000131
VERSION     DAFFFQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.136"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(515..856)
                     /locus_tag="LQB69_002811"
     CDS             complement(515..856)
                     /locus_tag="LQB69_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL3526915.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(846..1127)
                     /locus_tag="LQB69_002812"
     CDS             complement(846..1127)
                     /locus_tag="LQB69_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL3526916.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1498..1737)
                     /locus_tag="LQB69_002813"
     CDS             complement(1498..1737)
                     /locus_tag="LQB69_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526917.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1757..2005)
                     /locus_tag="LQB69_002814"
     CDS             complement(1757..2005)
                     /locus_tag="LQB69_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526918.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2020..2586)
                     /locus_tag="LQB69_002815"
     CDS             complement(2020..2586)
                     /locus_tag="LQB69_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526919.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2587..3132)
                     /locus_tag="LQB69_002816"
     CDS             complement(2587..3132)
                     /locus_tag="LQB69_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526920.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3158..3925)
                     /locus_tag="LQB69_002817"
     CDS             complement(3158..3925)
                     /locus_tag="LQB69_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBL3526921.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1290 a          959 c          597 g         1710 t
ORIGIN      
        1 cgcccgctcg ctacgcgacc gttcgttcgt cttttcctcc gtgggactca cccaccgcaa
       61 gcggttggtt gagttgtggc tcctattcaa aattcactaa tctgttattt tttatttcta
      121 ttcaaatagg taacgttcgt ttgcaagcaa actctctcta tgtaacttgc cagttacatc
      181 agcggtaacg ctacgtcgca agcgacttgc tctaatgaaa ctgccgtttc attctgcctg
      241 ttcgttcgta gcaagctact tcactcaccg tatccgtctg aattattgtt atttattgct
      301 attcaatatc cgtcagacat tttatttatt tacatacatt ttatataaaa tatatattat
      361 aattcgtata ttactaaata tcattattta acaattctta acgttttatt aatgtttata
      421 accacgtata tatttacgta tattttcata tacataaata cgtttatata aacgtatatc
      481 ttaatataca tataaacaaa aaagaaaagc cttgttagac ttttctttgt aatttctcga
      541 taaacctatt tagctttttc gtatcactag ctgataacat accaaccttt ttgaatgata
      601 tgttctctcg gcttttggga atagccttta aagaccctac gtcaatccat gattgctttt
      661 taagtcctgc tttttcccat tctgcaatag gaaaatacac cctctttatt tggtctgatt
      721 ttttctcata ttgacttgtg attccaaaga attctactct atcttcttta tcactaacca
      781 ccaaaatagg tcttctttta ccaccatttt tatctgtaaa tgcaacatat accgttacta
      841 tctcattagg cttcatcgaa ccactcttct aacttttcat ctgtatctag aacatctact
      901 ggcatgtcct tagttagctg ttgcaatctg attccttgct tctcctcttc acttagcttt
      961 atctctactg gaaactcacc tctagcgact acttgtttgt aaagaattgt tattgctgtt
     1021 gtaggattta aacctagctc ttccaatatc atttctgcgt tctcttttaa attcttgtct
     1081 attccgactt ggattctgct tttttcagtt ggatttttga ccttcatttc atccactcct
     1141 tttgagtata aaacactcat tatttatcaa attgtccaca tttatgatta tattatactc
     1201 ttaataaact acattgcaag taatctaatc aaattaagat gctatatcat acggtaattg
     1261 aacaaattgt cgctaaccca cacatacgga atcgtaatga atgaaaaaaa gacaagccta
     1321 ttcgcagtag gcttgtcttt gtgcttctct tgtacaaaat cttcacattt gtaaattaat
     1381 tttagcacct ttgttaggaa agaacaactt aaatgagatg ctaaagaatt aatagttctc
     1441 aatctagcat attcacttaa taccatctta tcttcattca tggttcacca cctcatctta
     1501 attattttca aattggcaaa tcaatttatt ttgggttaac aaaactaata gctgttcact
     1561 atcaaaaaga acctcacaaa attctttttc tatcctctct tctggcatgt aataacctat
     1621 ttgtttcaat atcttatctg gataaccttt tcgttgggtt tttctcaata aatcttcatc
     1681 atttccaaca taataataag aacaccattg cataccctta cttttaaatt ttgtcatgtt
     1741 tctatcttcc tttcacttat tgacccaacc atgaaaaaat atattttgct aaatcttcct
     1801 tttctattcc atataaaaca ttctctaaat ccaatttata ttcctcgtca acaaggcttt
     1861 ctgtatcgtc atcataaaga acatatactg cataatttct ttcgtaaaag gcattagcaa
     1921 gacttctgct aatacctacc attcctttcc atgtataacc aaaatcaacc aaatcttcat
     1981 gatctagaaa atccattcct tccatgctga ttcacactcc tattctgctg ttgtgtaata
     2041 tgcaacgcaa tcatctgccc ctactaacca cacacaccaa tcttcaattt catcatcgct
     2101 tctcttactc aattcctctt tggtacaaaa aactacttca ccattttctt tttccatctc
     2161 ataatcttgt agcatgtatt caaaccaact tctttcggtt ggaaattcta tctcgtggca
     2221 actctggcaa ataaaatcac catattcatt gattcggtag caaccttcat cttctacaaa
     2281 ttcatgatta caacaaacac atctagtacc tgttaggact tccccattaa tcgttgttct
     2341 aacggtatct tcccttgcta cgtgttcact gctgacaagc caatcaatga ttttctttgc
     2401 caattcttca tcagaatcgg caaagccttt aaacgtgttt ctctttcctt gaaagtagta
     2461 tttgcaactt gcggtagttt ctatggttcc ataacttttt tggaataatt caatcatttc
     2521 accatcaatc actttttgac ttttcgcttc taaattaggg aactggtctt ttcttttttc
     2581 aaacacttat ttcacgctct cttcttctag gtttattcca taattttcta gcaataatct
     2641 gtatccctta ataaaatctt tcgtagcaaa ctcttttccg ctttcacaaa acttgtttag
     2701 tcctatctgt tgaccctcaa aatcaaacaa tcgcaatctg gttttatcat ctagactaaa
     2761 tccattttca tcgaacacca tctctaaatg agcatagcca tcattgttat ctgaaaactt
     2821 catcacatct tcaatcgttt cttgataagg ttcatagcgc ccaccaaacc ctgtttcatc
     2881 ataagcactc actccagatt cataggtaac aaaattgata actcctgtac ttctatcaaa
     2941 gattcctttt gctaattctt ctcttgattt atctcgtaag tcgtatgctt tattgattaa
     3001 ataaattcct tgattcaata cgtccatcag catatatcga ccataccccc actgataatg
     3061 aatcgacacg cttgctttcg ttttgctgtt accatatttg actgtaatta acgcttgtgt
     3121 tctttcgccc atcttgtttt cctcttttct ctaattttta taaagaacca ttttcttgaa
     3181 atgagtaata gcttgtgttt ttacttgtta cgatgatatg gtctaacaat tcaataccaa
     3241 ttgtttttcc tgcgtttttc aaattcgtag taaaactttt gtcattttca cttggaaatt
     3301 catatccgct cggatgatta tgtgatactg cgatttttgc tgaattgttt agcaaagcta
     3361 gttgaaaaat ctctcttggt atcgctactg attgattaat tccacctttg aaaacttcgc
     3421 tataagcaat gacctcattc tttgtattca gacacatcac tagcaaagat tcactagcat
     3481 tgtttccaat taagttagta gcaaagtttg ctagaccaac cgagcttgtg actctataaa
     3541 aacttctatc ttcactgata tatttttgtt cgattttcaa gacattctta atcaatcctt
     3601 caccttcatt ctcggtcttg ttttcaactt caatagattc aagactgcta tttcccatat
     3661 cagaacccaa caaagcattt ctgaaatcca ataccttttc aaagttatca gtcataacca
     3721 tttcataggt gctattccat tcattcaaat atttctcttt gatgagaatg aacgctctcg
     3781 gtttacttct gccaattctg atattttgtg tatcgtgaaa aatatagtta tcaagtaaag
     3841 taccatcgta cttttcatag ttcccactga tatttgttaa cttgtttacc atttcttgat
     3901 taacttctaa aattctatct ctcatgttga acgcttcttt ctgtttttga tttttggttt
     3961 ttcaaaattc ttttttgttt ggattgtttt agagggaggt ggttttttct tcatcgctat
     4021 ttggttttgc attttatgtt tcttcctttc gttcatttat ttatcttttt cgcctgtctt
     4081 gccttgtcgg gagtgattag aatgcgttct tatctgccgg attaatacaa gtgtttaagt
     4141 aaattatttt cgtaacgctt gcgtttagaa aaaaatttac gccacttgta ttaatccaaa
     4201 gatgagataa gctctaaaca cttctgacaa ggtatgacag gcgaaaaaga taatgggcgt
     4261 aaggaattat aaatggcaaa accaaataaa gagcgatgaa tttcaatgct ttcaggcgta
     4321 gcttgaaagc ctaaaagtct tgaagaatga aagacttttt accttcaaag cagacctagt
     4381 aatcttaaat aggcgatggc tatttaagat tacggtgttg acccactaaa taaacagaaa
     4441 tggaaccgat caagcaaaat cggactaggc gatggctagg acggtttgcg gtggctttag
     4501 gaaacgttca tggtgagatg agcatatcta gcaatgtcgg gctaagctat gcttag
//
LOCUS       DAFFFQ010000132         4473 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000132
VERSION     DAFFFQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.137"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..462
                     /locus_tag="LQB69_002818"
     CDS             <1..462
                     /locus_tag="LQB69_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBL3526922.1"
                     /translation="GERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAA
                     SEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEIIQ
                     NSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            478..627
                     /locus_tag="LQB69_002819"
     CDS             478..627
                     /locus_tag="LQB69_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526923.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            701..1294
                     /locus_tag="LQB69_002820"
     CDS             701..1294
                     /locus_tag="LQB69_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL3526924.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1310..2209
                     /locus_tag="LQB69_002821"
     CDS             1310..2209
                     /locus_tag="LQB69_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL3526925.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKKLYNQEMPQLMKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            2212..2697
                     /gene="ssb"
                     /locus_tag="LQB69_002822"
     CDS             2212..2697
                     /gene="ssb"
                     /locus_tag="LQB69_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3526926.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2719..2976
                     /locus_tag="LQB69_002823"
     CDS             2719..2976
                     /locus_tag="LQB69_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526927.1"
                     /translation="MAKKNSLTMDLLIPVIAFIDGISLGITENLLVNRIAKGSLFLVF
                     VYLVCSYLLKRVGKDNSFWRLTKVTIGATAVILTAILPLYM"
     gene            complement(3136..3258)
                     /locus_tag="LQB69_002824"
     CDS             complement(3136..3258)
                     /locus_tag="LQB69_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL3526928.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3412..3672
                     /locus_tag="LQB69_002825"
     CDS             3412..3672
                     /locus_tag="LQB69_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526929.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLHTSHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            4115..4315
                     /locus_tag="LQB69_002826"
     CDS             4115..4315
                     /locus_tag="LQB69_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL3526930.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PICGKKYTLSYGDYILIEEKGR"
     gene            4320..>4473
                     /locus_tag="LQB69_002827"
     CDS             4320..>4473
                     /locus_tag="LQB69_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526931.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIE"
BASE COUNT         1630 a          772 c          878 g         1193 t
ORIGIN      
        1 ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt tgtctctcac
       61 ttattggaag tcgtcccagg acaaatccaa tccactgatt ttggatcgac gttacaagaa
      121 gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc
      181 caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga agcctgtgat
      241 tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct gaaggagatt
      301 attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa gggttcgttt
      361 gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc tgaaaagaaa
      421 aaaacgaact tcaagaaaag aacacgacgc actacaaaat aatggagggt taatcttatg
      481 cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat tcgttcagaa
      541 caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat taaagaacca gcaacggatt
      601 caaaaagaaa ttgaattaga tcgataaata tctttcttgt taatataaaa ctaacgtgat
      661 atagttatgt tagttttata ttaactttag gggaaaagca atgaaagaca aaatccagtg
      721 catttttgat aaacataatg ggaatctatc cttgaaggat gctagaagtg aaggcttatc
      781 gcctatgacg ttagatcgat tagccgaaaa ggacaaaata gaaaaggtag caccaggttt
      841 ctatacctta aaagaagcgt tcgtggatga attgtatctc gctcaaagta tctattccag
      901 aggtattttt tctcatgaga ctgcgctcga tctttttcaa gtcggaacgt atatccctaa
      961 aaagatcaat atgatgtttc ctaaaggata caatgtttca aaggagaaat tagacgagta
     1021 tgctattcaa ccgcattaca cgaataaaga aaattttgac ctgggaatca cagaaacaga
     1081 aagcttttac ggcaataaaa ttttagtcta tgatttagag cgaacgttat gtgatatgtg
     1141 gaatccaaga tacaaagcaa gcgtagaggt aaaacaagaa gcattaaaag aatatatgac
     1201 aagtaccagt agaaatacaa ataagctaag aagatacatg gatattttaa aatcaccaaa
     1261 agaaatgact ttgtatatgt tgccacttta ttgagaggag taaaggtgaa tgacaccgac
     1321 acaatttaaa gcaaaaacga gaaatttcgc aacagagaaa catttaaatc cacaaattgt
     1381 ccaaagatat tatatgatgg aaaaattatt agaacgtatt gttgaatcgc cttatcatga
     1441 tgattttgta ttaaaagggg gatttctgat cggatcaaaa tacgggttag aaaaccgttc
     1501 aactgttgat attgatacga cttatcgaaa ttcgaaacta acaaaagaaa agttagaaac
     1561 tgttttttct gagttaacag ctgatcccac aaaagatgga attcgatttg aattaaaggg
     1621 attaacagag acgagggaag ccgattatta cccaggtttt caagcaaagt ttattgctta
     1681 tcttgagaat gcacgtgtac catttaaatt agatattact tcaggtgatt ctattattcc
     1741 agcggcattg atgtatgaac acaaattaat gtttgaagat aaaacgattc atattccagc
     1801 ttatcctacg gaacaaattc tagctgaaaa attacatgct acgtttagtt tcggaaaaga
     1861 taattcgcgc atgaaagact tttatgacct gtatatgatt ccaaaactag aacgtatcga
     1921 tgagaaaaac ttgtataaga gtgttcaagg aacgttcaag gaaagaaata atcaatctgt
     1981 gtttaaaaaa ttgtataatc aggaaatgcc tcaattgatg aaggatgagg ggttgaggga
     2041 aaagtggaaa agataccaac aagaaaatta ttttgccaaa gacatttctt tcgaatctac
     2101 actaacttcc gtcgattctt tgatgcaatc aattgtagag caagagaaag tagaacgaaa
     2161 acaaaatttt aagagatcac aacaaatgaa tcaagaaatg gagagataaa aatgattaat
     2221 aatgtaactt tagtaggacg attaaccaaa gatccagatt tacgttatac tgcaagtggt
     2281 actgcagtag cgacttttac cctagccgtt aaccgaaatt tcacgaatca aaacggcaat
     2341 cgagaagctg attttatcaa ttgcgtgatt tggcgcaaac cagcagaaac gatggcgaca
     2401 ttagctaaaa aaggcagctt gatcggtgtt gtcggacgga ttcaaacacg gacttatgac
     2461 aaccagcaag gacaacgtgt gtatgtgact gaagttgtag cagataactt tcaactatta
     2521 gaaagtaaag cagcgacaga gagccgtgcg catgcagatc aatcgtccac cagtccaagc
     2581 accgccacct ttgaacaacg ggatacagcg acgcctaaca acaatggatt gaacgcttca
     2641 cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttc
     2701 gagaaagaag gagtaagcat ggcaaaaaaa aatagtctga cgatggatct attgataccc
     2761 gtaattgcat ttatagacgg gatttctttg ggtatcactg aaaatctttt ggtaaataga
     2821 attgcgaaag gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttctaaaa
     2881 agagtaggaa aggataattc cttttggcga ttgactaaag tcacaatagg cgcgacagct
     2941 gttatactta cggctattct accgctttat atgtagggtt aacgggaaat cattgaactt
     3001 cttgcgaaat attgttataa tgaacatgaa aaagaggagt tgcgcaaaca acccctcatt
     3061 aacagcaccg tttaaagaac ggcggctcag attaattatt tattgagaaa taaccgatcc
     3121 tcgctaaagt gacggttatt tctttttgtc atttttaagc atatttacaa tcaaactaat
     3181 caaagcaatg gtaaacatac caaaaccaag aatggtttga attgtctcat atgcggacaa
     3241 aaggcttact cctttcatag attttagcac ttacattcat aagcaccacc accttccaga
     3301 agtcgccacc gtcattaaac tttactgtct tgatcattat agcatatcta aaatcgaata
     3361 aaaattctca attttagctg tttttgctat aattttagcg aggtgatcgc gatggaacat
     3421 tatactggtt tactcgataa aattcgggtg atcaagacac agccattgct tgtgcgattt
     3481 acattacaca cgagtcatga accaatcaat tgtgtagtgg ccaatataga aatagcggat
     3541 aaattgctga ttttggaaga aggcaagtat actatttcag tgacaggtca cttcaataaa
     3601 cggaatcaat tagtcattga atcgatgcaa attagaaacc ccgatcattt tacgcgatcg
     3661 atgggcatat aaaagaagag ctgcaatcct aatgtaggac tgcagctctt tttgttttac
     3721 atagaaacgg gaacaggaaa aggccttttt aggaaggcac aagtgccttc tcaattgaaa
     3781 aacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgctgcac
     3841 gttcactaag ataaggggtc ttgcaaagca agacaaaata gaaaaaactt cctctcacca
     3901 aaaggttcgt tcctgttcgt tatctcttta ttgatttgcc agaccggcaa caagttgcct
     3961 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtccttg
     4021 tcgcttttcg ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa
     4081 gtaaaacctt tcctaagagg aggcagaatc aaacatgcga aagaaatttg attattgggg
     4141 tgttcccttc agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg
     4201 tggagaaaga gcgaaatgta tcaaaagcta tcgtttgtat caatgtccaa tatgcgggaa
     4261 aaaatatacc ttgtcttacg gcgattacat tttgattgaa gagaaaggaa gataaaatga
     4321 tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa
     4381 tggaggatat gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa
     4441 aattgtcggt agctgtggaa gaaatgattg aat
//
LOCUS       DAFFFQ010000133         4469 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000133
VERSION     DAFFFQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.138"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(462..887)
                     /locus_tag="LQB69_002828"
     CDS             complement(462..887)
                     /locus_tag="LQB69_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526932.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1261..2232
                     /locus_tag="LQB69_002829"
     CDS             1261..2232
                     /locus_tag="LQB69_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL3526933.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2264..3661
                     /locus_tag="LQB69_002830"
     CDS             2264..3661
                     /locus_tag="LQB69_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL3526934.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3820..4443
                     /locus_tag="LQB69_002831"
     CDS             3820..4443
                     /locus_tag="LQB69_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526935.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1565 a          717 c          911 g         1276 t
ORIGIN      
        1 aaaaattact acgtgatgta gaataagcct ttctatcgtt tggttatttt atattcaaat
       61 atttgaaata aataaatctt tactctttga tttgtaaact gaaatgatcc actgtttgtt
      121 ttactgatga agcttgatca aaggaagaag ctgagtttga agttcataaa aatcagccat
      181 agtgagtttt gcttggattt gtttatcttc ttctactttt tacataattt tgaacaggat
      241 aagattcaaa tgttttaaat gaatctttga ctagtttgtg acagttgtcg catatgctca
      301 agcttccttg gtcattaaat tctgtatgga tatttttgtt gttattacat gtacaataat
      361 caggactatc gatcatccat ttcgtctcct ttacaatcat cttgcttctt tactatagca
      421 gaaaagaagg ggagaaacaa aagaagatcc aacagacggc actatagact gtagatgttg
      481 ccatcctcat ccttcaaacg gtaattaccg atttttgtaa atatgactgt tgtttctttt
      541 ttattttctt ttcctgaaat aatatattcg tcatctttgg tagtgatctt gatatctgtc
      601 agcactgttt tttcttcaga tgattcagaa gaatcagttc cttgtgtctg atacgtgcgt
      661 gtccattcca tttttttatc tgtaaatgtc aactgtcccg cggataaatc catatcttta
      721 cgtactttta gcgtatatac actgttggtg gtatctgctt tttccgcttt taagacagaa
      781 gaatcggttg tttcgtccga ggattggtca gagccgccaa acaaatcaca gccagtcaat
      841 gtcaaaagtc ccgcagacaa aataaatagt acgattaatt tcttcaaaat acttccccca
      901 tttgatagga taaagaaatt ataccattgg aatgtaaaac ttcaaacgga tggaagagaa
      961 aaacgcacaa aacacccaca aatagagtaa attttagaat aaaattttaa aaaggcaaaa
     1021 aatccgccac tttcagaaag cgttttaaaa aagtgttgca atcttttaaa aaaatgctat
     1081 attaaaaatg aaccggttca aatacttgtt taagtataat taatagtgag aggaagttgc
     1141 tctactgaag actttagtta tatggatatt gatgacaagc aacactatgc aaagaatgcg
     1201 gtcatcataa tcgaacaaca agaaagagaa atcaaatgta actggcagga gtgagaatgt
     1261 atggcatcga tccgtgatgt ggctaagatg gctggtgttt cagtgggaac agtttcccgt
     1321 tacttgaatg gtcagcaact gaaggaaaag aacatgaaaa aaatagcaga agcgatttca
     1381 gcgttggact ataaagaaaa catcattgct aaagggttga agaataatcg aagcttctct
     1441 attggacttt tgataaacgg gatttccagc cgttttgggt cggaagttgt ctcagggatc
     1501 gaaagagtag ctgaagaaaa tgggtatagc ttattattaa gcgggtttgc cgatcagcca
     1561 gaaaaaattg aacaaaaaat tgaatacctg atgaagcatg tgatcgatgg gctgattgtc
     1621 ttcttatccg aagaagagtg gtctggattt gaaatgctca caaaaatgag tattccagtt
     1681 atcgcattga attgtccaga aggaccagca ggtattgata caatattagt gaatgatcga
     1741 gaaagtgtag caaaagtgat cgctcatcat ggtgaacaag gacataaaaa aatcggattt
     1801 attgctgcca ctcaaacaga ctacgtagca agagaacgtt tagcaggagc caaagaagcg
     1861 gtccaaaatg atccatcgat ccaattagag gtctttacag gagactattc tagaaaaagc
     1921 ggctattacg gggcaaaagc attattagaa aaaggggcaa ctgctatttt tgtcagcaat
     1981 tacaatatgt cgattggtgc catcgaatgt ttcaatcaag aaggtgtcag aatcgggaag
     2041 gatattgcgt tcagtcacta tgattattta gataaaaacg atctgagtat ccttccgaaa
     2101 atcacgatca cgccaccaac agataaaatc ggggaacgag cagcagaaca gctgttaaaa
     2161 cgaatcaaaa aagaagagga agaaaatgaa aaagtgattg tgatggaaaa tatcatacat
     2221 ggaatcaact aaaacgaact aaaaaagaaa aagaggtgct ttgatgaaaa aatatcaatt
     2281 tccagcaaat ttttggtggg gttctgcagc aagtggccca caaacagaag gaaggatttc
     2341 aggcgatgga aaaggagaga acatttttga ttattggtac aaaaaagaac cgcaaaagtt
     2401 ctttgatcaa gtaggacctg aaaaaacctc gcaagtttat acgaagtatc aagaggatgt
     2461 acagttgatg aaacagactg ggcataattc atttcgaaca tctatccaat ggagccgttt
     2521 gatacctgat gaatctggaa caatcaacga agaagccgtt cgtttctatc atcgttattt
     2581 tgatgagttg attgaaaacg gtatcgaacc ctttgtgaat ctgtatcatt tcgatatgcc
     2641 gatgtatctg caagaaaaag gcggatggtt gaaccgagaa acggttgaag cctatgaaaa
     2701 atatgccaaa agttgttttg aattgtttgg ttcgaaagta aaaaaatggt ttactcataa
     2761 tgaaccaatc gttccagtcg aagcaggtta tttatatcga tggcattatc cagaagagtc
     2821 agatatgaaa aaagcgatcc aagttggcta tcatgaagcg ttggcttcag cgttagccat
     2881 caaatcttac catgaaatgg agcaaggtgg tgttattggt atcatcttga atctgacacc
     2941 aagctatcca agagacgaat caaaccctga agatgttaaa gcagcgcaaa tagcagatgc
     3001 gttcttcaat cgttcattcc ttgatccagc tgtaaaaggc gagtttccaa gtgaattgat
     3061 tgagatagta aaagaacttg atctcatgcc tgtgatccat gcagaggatt tgacggttat
     3121 caaagaaaat acagtagatc ttttaggtat caattattac cagccacgcc gggtaaaagc
     3181 aaaagaaaca ccaatcgata ctaaaaacgg tccaatgcct gaggattatt tcgacaatta
     3241 tgaaatgcct ggtcgtaaaa tgaatcctta tcgcggatgg gagatctatg aacaaggaat
     3301 ctatgatatt ttgatcaatg tacgagatca ttatggaaat atcgactgct ttatttctga
     3361 aaacggtatg ggtgtagaaa acgaagcacg atttatcaaa gaagatggca tgatccacga
     3421 tgattatcgg atcgaatttg tccaatctca tctgaaatat gtccatcaag ccattcaaga
     3481 aggtgccaat tgtttaggct atcatatgtg gacatgtatg gataactggt cgtggaccaa
     3541 tgcatataag aatcgatatg gctttatttc agtagactta gcacaagatg gaaaaagaac
     3601 aatcaaaaaa tcgggtcatt ggttcaaaga cgtatcagat aaaaacggat ttgaggctta
     3661 agaaagaata gttgagagca aatgcgtgaa attctccatt cttaccattt tgtgaaaaag
     3721 aagttcaatt atttacgatc tttttcaact tttttgtctt tctatttcat tttttgtata
     3781 taatagaata gtcaaaatta aataggagtg gaggaaagca tgaggaaaaa aagaccgttt
     3841 cttctctttt ggttattcgg ttttttctta ctgcttattt tatccgcagc attttcagga
     3901 aaaatgacag cagatcttgc tcagaaagaa acagaaaaaa tcgtagcaag caaagaatca
     3961 gtagataatt tcaaaaaaga attctcaact tattggtggg cggagtataa aataactgcc
     4021 aattacgaaa aaggcgaatt ggacatttat ttgccaaaag gaaaagaaga aaatcaattt
     4081 attaatatcg atgacgtact tagtgcgatt tctctcagac aatatcaatt taaagacaag
     4141 catttgaccg ttttgctttt gactcatgat ggcgacaaag tagcccgttt gaaaaatgga
     4201 aaagtgatca ccgcttattc tgaaaaagta gacaaaaaag tggtcgccga tcgtttagcg
     4261 gaatggaaaa agacaaatga acaagtcaag aaaacaactg tctcttctca aaaaaacgag
     4321 tcgacaggaa caagtccttc agattctggc agtggacaaa cgataacggg aagtgaagaa
     4381 aatcaagcct caattattac gaatgagtca tcacaaggac aatatcaatc ctatggacaa
     4441 tgaaaaacag aggttgtgaa atcagcgtt
//
LOCUS       DAFFFQ010000134         4453 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000134
VERSION     DAFFFQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.139"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            878..2203
                     /locus_tag="LQB69_002832"
     CDS             878..2203
                     /locus_tag="LQB69_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL3526936.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            2196..2543
                     /locus_tag="LQB69_002833"
     CDS             2196..2543
                     /locus_tag="LQB69_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526937.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2858..3715
                     /locus_tag="LQB69_002834"
     CDS             2858..3715
                     /locus_tag="LQB69_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL3526938.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            3735..4295
                     /locus_tag="LQB69_002835"
     CDS             3735..4295
                     /locus_tag="LQB69_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBL3526939.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         1686 a          600 c          828 g         1339 t
ORIGIN      
        1 aagatatggg atagaagtca gaagttagta aattaaacaa tcgttgattt ggcaacgaaa
       61 tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg accagacgga
      121 aggacgaatg ttgttgaggt tttagaacca tataaaacag catgtttctc aaatttttag
      181 gataagggaa attgctagca gtttgtttta gaaccatata aaacagcatg tttctcaaat
      241 gagtacttta aattaattga tggcgttcct gctttagaac catataaaac aacatggatt
      301 taaaacaaga agtaagtcag ttctcctttt actttagtaa ggaaaaataa agtattctca
      361 ataaaactta aaataagcaa acctttgttt taatagctaa tcgaagtaaa acccaaagcc
      421 atatgaaccc ctgaatcagc gaaagtttat gatgatttgg attactaata gtttaggaat
      481 tagatggtaa atctaaatta aacaatcgtt gatttggcaa cgaaatagtg agggaaaatc
      541 cactcactga agggtaaact ccgtcaagtt atgtgaccag acggaaggac gaatgttgtt
      601 gaggttttag aaccatataa aacagcatag ttctcaaatt gtttaaaatg attgtcaagt
      661 taaaaaaatc tgggatcata taaaatagta tagttctcaa atatgattag aaaccctatt
      721 aaagagcaat attaagccaa cttacttaaa tcgtttaatt aagaacaata atctctttta
      781 gtcagacttt tagtctgact tttttgtatt tccaaaattt attctttaca aaaacgaaca
      841 tacgtttgta taattgcttg tgaatggggg aataaaaatg tttgattaca gtagagaacc
      901 ggtgaatgac tattttttaa tcgacatgaa gtctttctat gctagcgtgg aatgtattga
      961 acgaaattta gacccaatga ctacaccgtt aattgtcatg agtagatcgg acaacactgg
     1021 ttcaggtctg actctagctt cttcacctac tgcaaagaaa aaatatcata ttactaatgt
     1081 tagtagagct actgatttac cttatccatt accaaaagaa cttcatattg ttccaccacg
     1141 aatgaactta tatataaaaa aaaatatgga agttaatcag atttttagaa agtatgtcgc
     1201 agatgaagat ttattgattt attcgattga tgaatcaatt ttaaaagtga caaaatcctt
     1261 aaatttattt actagttcag gaacaagaag cgagagaaga agagaattag ctaaaataat
     1321 caaagatgag attcagaaag aaacaggtct aatagcaaca gtcggaatag gagataaccc
     1381 attattagct aaacttgcac tggacaatga agcgaagagt agtaaagaat ttatagctga
     1441 gtggacttat gagactgtac ctgaaaaaat atggaaaata aacgagatga ctgacttttg
     1501 gggcattgga tcacgcatga aaaaaagatt gaatcatatg ggaattatga gtatagaaga
     1561 tttagcaaat tggaatccct acacaattaa aagtcagtta ggtgtaatcg gactccagtt
     1621 atatttccat accaatggaa ttgatcgtac ggatattgct attccaccag aaccaaccaa
     1681 agaaaagtct tatgggaatt cacaagttct accaaaagat tatacgagga gagaagaagt
     1741 agaactagta gtcaaggaga tggcagagca agtagccatt cgaattagac aacacaattg
     1801 caaaactggt tgtgtacgct tagcaattgg tacgtcaatt ttggaagaaa aatcaggttt
     1861 ctcgcaccag atgaaaatac caattacaga taatacgaaa gaattacaaa attattgtct
     1921 ttttttattc aataagtttt atgaggggca ggaagtacga catataggag taacttatag
     1981 cagattagta tacacagatt ccctacaatt agatttattt agtgatccaa gaaaaaagat
     2041 tgctgaagaa aatttggata ggattattga taagattaga caaaaatatg gatttacagc
     2101 tattatacat gcttcatcta tgttagaagg tgcaagaagt attacgagaa gcactttaat
     2161 aggaggacac gcaggaggat taggaggttt gcataatgat tgaatataac gatagaggac
     2221 gtttaaaatg gtcgggattt tatttgtctg atcatactga aagaatcaat gcagacaatg
     2281 tacaacgtgc taatcataat ttggcaaaag aacaaatgac gactgaagag attgcgaaaa
     2341 tactgagaac tgctgtcata aaaaatagac ccgtctgcat tcaaaaagag gaacgaaacg
     2401 ctgaaggtta ctatccagat gatgtagtag gaaaaattat tggatatgac gagcttggtg
     2461 tatttgtgga agaagaaaaa gttcactatg atgaaattag gaatgtttct ttctcggaat
     2521 tattaaaatg gagtcagata tgaacattat taaaaaatac gaattcggtt atattacgta
     2581 tgaacagcta atagaggaat tatggggata tggtcaacaa ttaattaatg aagtggggat
     2641 tgattgcttt tgtttctata ttgaatccgg agcaggatac cataagaaca gatactatat
     2701 atttccatat ccaccaaaat agtctcttag atatttttaa attttctcca aatactttca
     2761 attattgtga aatatttccc tttaattaat caaatttttt ataaaagtaa tatataaaat
     2821 aaattgcttg aagtattata tttagatatc ataatttatg gaagaaaaat taattaaaaa
     2881 ggatgagatt ataatggggt tactggttcc gagagctaat acttatacta tacctttaac
     2941 gaaacgccaa ttaattgcta aaagatatca aaggattact agagccatca atagagagtt
     3001 ttggaattct gaaagtgata ctgctcacag tttatacgta ggttcttatg ggagaggaac
     3061 ggcaattagt actagtgata tagatataat agttgaatta ccgatggcag aatttgacag
     3121 attcaaaaat tatttatcaa atggcccgtc taagttatta caagttataa aaaacgcatt
     3181 tcaggaaata ctaccaaata gtgatattag agctgatgga caagttgtta aaataaattt
     3241 ccatgatgga atcaaatttg aaatagttcc agcttttaat gaaaaagatt attggggtga
     3301 gtcaaaaggg tttatttatc cagattcaaa catgggaggg aactggaaag ctacaaatcc
     3361 caaaaaagag caagaagcca tgaaattaaa gaatacaaaa agtaataatt tattatatgc
     3421 tacgtgtaaa cattttcgac atgtacgaga tacagaattt acgagttatc atctttcagg
     3481 aatagtaatt gacagtttcg tatatgaggc tatgggaaat tggaaattcg ttgaaaataa
     3541 cagtgggggt caaaacatta gttctgtatc ttatgagaca gctttattag aatactataa
     3601 ctcacataaa gttatgggcg gtttaaattt atactctcct ggaagcaatc aatttgttaa
     3661 ttctgatagt agtatcatat gtttagaaaa agtattaaaa aaaatagctt tataaagttt
     3721 tatggaggaa caacatggga gacgaaaaaa agcgcttgga agttcaatta cgtgaagcat
     3781 atggacgagt aacgtatact tacaccactc atttaaagca agccgataat attatttctg
     3841 acaataaaat tttaaggtgt cttcaaattg gtttgtcagc ccttactact ggaggggtac
     3901 ttagtaccat tattactgat aatagtgagt taacctttgt tgctggatta tgttcaacaa
     3961 tttctcttgg attaaactta tactttaaga attttgatct tgctgaagaa gctaaaaatc
     4021 atcaaattgt tgccgataaa ttatggttga tcagagaaga atacgtaagc ttactaacag
     4081 atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag agatcaatta atgaacgaaa
     4141 ctcatgaagc atataaaaat gcccctaaaa caaatagtaa aagctatgaa tcagctcaga
     4201 aagcattaaa atctgaagaa gagcaatttt ttacagaaga ggaattggat aagatgttac
     4261 cagagcatct tagaaaaaag agttctaaat attgaactct ttttttatgc ttaaaattct
     4321 cttgtaattt ttctattcat ctatatttaa gaagaaatat gatgatagaa acatctccta
     4381 aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagata tgctcatctc
     4441 accatgaacg ttt
//
LOCUS       DAFFFQ010000135         4431 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000135
VERSION     DAFFFQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.140"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(543..2048)
                     /locus_tag="LQB69_002836"
     CDS             complement(543..2048)
                     /locus_tag="LQB69_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL3526940.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LQB69_002837"
     CDS             complement(2045..2731)
                     /locus_tag="LQB69_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL3526941.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LQB69_002838"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LQB69_002838"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL3526942.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat a
//
LOCUS       DAFFFQ010000136         4401 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000136
VERSION     DAFFFQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.141"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(99..1619)
                     /locus_tag="LQB69_002839"
     CDS             complement(99..1619)
                     /locus_tag="LQB69_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL3526943.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1862..2047)
                     /locus_tag="LQB69_002840"
     CDS             complement(1862..2047)
                     /locus_tag="LQB69_002840"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HBL3526944.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="LQB69_002841"
     CDS             complement(2031..2213)
                     /locus_tag="LQB69_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526945.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="LQB69_002842"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="LQB69_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="LQB69_002843"
     CDS             3167..3766
                     /locus_tag="LQB69_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3526946.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4401
                     /locus_tag="LQB69_002844"
     CDS             3830..>4401
                     /locus_tag="LQB69_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage abortive infection AbiH family
                     protein"
                     /protein_id="HBL3526947.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1403 a          799 c          785 g         1414 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag a
//
LOCUS       DAFFFQ010000137         4306 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000137
VERSION     DAFFFQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.142"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            149..1138
                     /locus_tag="LQB69_002845"
     CDS             149..1138
                     /locus_tag="LQB69_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBL3526948.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAERLNDEIDLFNHALSKMFRRKKVQIAIKGYVRKLEITYNKEREDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGVNPDGTDEITQLDVRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1135..1554
                     /locus_tag="LQB69_002846"
     CDS             1135..1554
                     /locus_tag="LQB69_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526949.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1533..1871
                     /locus_tag="LQB69_002847"
     CDS             1533..1871
                     /locus_tag="LQB69_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526950.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1875..2237
                     /locus_tag="LQB69_002848"
     CDS             1875..2237
                     /locus_tag="LQB69_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526951.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2459..3865
                     /locus_tag="LQB69_002849"
     CDS             2459..3865
                     /locus_tag="LQB69_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3526952.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1501 a          602 c          996 g         1207 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt gatttatagt tttaattgtc taagtcaaaa
       61 ctaaactaga aaggagagca atcttaattt ttgttatcgg cttttcagcc ttttttgtat
      121 acaaaatttg tagattcagt atagcaagat gaaaaagaaa aagcaagatt tgattaacgg
      181 aacatttcga gaaaaaaaga aaaatttgaa aaaagtggtg gcatttagtg ataatcgaat
      241 ttctcccaga atgcgtgaat tgatgacagg ctgtggttca tatttagaat ttattgcaac
      301 gattgataag gagaaaaaga aattggtcca agctcacttt tgtaaaaatc gtttctgtcc
      361 gttatgtgct tggcgtaaag ctcgtaagga ttcgatgatg ttgtcgataa tgatgcaagc
      421 cattgcggaa gaaaagcagt atcaatttat ttttatgacg ttgacgacac caaatgtgaa
      481 agcggaacgt ttgaacgatg aaattgattt atttaatcac gcattatcga aaatgtttcg
      541 tcgaaaaaaa gtgcaaatag cgattaaagg ctatgtgcga aaattggaaa tcacatataa
      601 caaagaacga gaagactata atccgcattt tcacttgatt ttggcagtaa ataaatcata
      661 tttcacggat aaggattatt atatcaagca atcggaatgg ttagatttgt ggcgggaagt
      721 gactgggaaa acaggggtca atcctgatgg tacagacgaa attacgcagt tagatgtccg
      781 taaggtcaaa ggattccaac aagaaaaagc agtacaggaa gttgcgaaat attcggcaaa
      841 agattttgaa ctgaccgaaa gtcaagaagt gtttgacaca ttctatcatg caatgaaagg
      901 tcgtcagttg attacgttta acggtgtatt caaagaatac aagaaaaaat atgagtcggg
      961 tgaccttgat cgttataaga aaaaagatga gaatgaatat ttttggtttt tgactgcttc
     1021 gtggatgagg aaagaatcga aatatgcggt tgattatcgt gagatgagtg ctgatgaaag
     1081 aaattatttt tcaggaatgt atgtcgatga aaaatctgtt gagggggaaa atgaatgact
     1141 gattcaacgg ataaaatgac aattaaggcg cttgctgatg aactaggaat aaccaaaaat
     1201 acagtgagaa attatctagt caaagccgga tatgatcttg ctcggtacaa gaaaaatgga
     1261 attatcttgc tagacaaaga gttgattggt gttgctcggt caatttatcg gtcaaaaggt
     1321 ggtcaattga ctggtcattt accggtcaat agcacgggca gtgattttcc tttggaaaaa
     1381 gagaatgagt ttttgcgtga tcaattagct gagaaggaca aacatatttc tgaattaaca
     1441 gaaatgattc gtgatcaaca aaaattgacc ctgcttcagc aacaaaaaaa cgatcagttg
     1501 ttactagaaa atcaagaatt aaaaaatcga aaatggtggc aattctggaa gtgagatgtg
     1561 acatggataa aattgatttg agagaagaaa cagtcaatag aattgaagtg atgtatgaac
     1621 ggttagctga ttatttgaat ctttcggatg aagataatta tagtgagatg cttgaacgaa
     1681 tgatggatgt tctcaactcg gttatcaatg ctgatctagc aaaatcttca gttttgacta
     1741 atgaatttgt tcataagcat ttagcgcaaa caactgatga agctgaaaaa gaattttggt
     1801 tgaaattaat gagtcatatt atgcagttaa accaaatttc cgttattaat tccaaagaaa
     1861 aggggatgta gtttatggga atgactttag cagatattaa agtgcaagaa gaaaaattaa
     1921 aaaaagaaca ggcgaaactt cgccaacaga agaaacgatt gattgagaaa gaaaatgctg
     1981 caatgggcaa ggctcttcgc aagcgttttg ggtttgaatc ttttgctgat tttgagcggt
     2041 ggctatcgtc gatttctgac gatcagcaac acgaaaaatc agtgggtaat gagttgcgag
     2101 ctgatgaaaa atcagcacca agcgaacgga attaccctaa cgaatctgac ggaatattcg
     2161 gagggatacc gaatagaaaa agtcagatga gtgagagtgg atttgcgtca atgtttagtg
     2221 acgtaaattc acgataagcg aatattttgc atgaagcaaa ataaagcgta aaaggagttg
     2281 agagcgtgac acgctttcgg ctcctttttt cgttgtcact tgtgacggcg aaaaccctcg
     2341 gagagcgttt ggaattgtga cagtcatgtc acaattagga aaacgttact ctttgacaaa
     2401 gagtaacaga aaggtttaca ctctttttat tagcgacagc gcgaaggagg ggtttaactt
     2461 ggggtttagt atttcgttca aaaagggaac aaagaataca agcatttctc ataataatcg
     2521 tgagttaact gatagccaaa aaatcaatga ctggcataag catattgatt tcacaaaatc
     2581 ggatgaaaat atctatttgg aacaaataaa tattcgtgaa aaatatgaag aattattcgg
     2641 tgaagccgta gaaaaatata atgcaaagca gaaaagagcc gatagaaaaa ttgaggacta
     2701 tttagcaaag gttcgcaagg ataaaaaaat ggaaccacag cgagaattta tcgtgcaaat
     2761 tggtactctt gatgattttc gtactactcg tgatgatggt tcttctactg gcatatcgga
     2821 acaacaagca gaacaaaatc gagtgattgc caacaaaatt ttagttgagt atttcaaaga
     2881 atttaaagaa cggaatccta gtttatccgt ctacaatgct gttattcata atgacgaagt
     2941 aagtccgcac ctgcatttga atattgttcc tgttgctgaa gggtataaac ggggtgttca
     3001 aaagcaacca agttttaaca aagccttgca acagcagggt ttacgagcag ataaagagaa
     3061 tggtcgagtg atttggaata attttaggaa tcaggaagtt gagtcaattg aacgtttgat
     3121 ggcagatttt ggttgggagc gtgagttagt cggtactaac aaaatcaaag atattcatga
     3181 atacaaagaa atcatgagcg aaatttccga attgcgtcaa acggccgtaa aagagcgtga
     3241 gagcgtttct gatgagttga ggtatatttg taggcaaaga gaagaaaacg agcgtgagaa
     3301 ggctctggtg gcttctgaga gagcgaagat tgaagaagag aagaaaaata tgctgaatgt
     3361 aaaaaaagag atggcggtca ttttccaaaa agatatggtt cctttagaaa gtttcaagag
     3421 gcttcctgat ggttcgtatc atttatctgg gaatgatttt gttgatttgt atcaacgagc
     3481 tacgagagca acaaatgcca agagaaatgc tgataacatg agtgagcaat atttacagat
     3541 gcttgagcaa aatacagaat taaatgagta tgtagctgca ttggaagaag agcgtcattt
     3601 gtctggtaga aaacaattag cagatttaca agtggaaaat aaaaagctga aagaagaaaa
     3661 tcgctcttta cgccagcgtt tagagcgatt aaaaaacgca tttcaaaaat cgatcactcg
     3721 tttgtctatc cgttttgggc ttgcagaaaa ggatatgggg ctttctgagg aattagaagt
     3781 attagagcag gagaaaacct tattttatcg tcaaaacgtc tcacagcata aattagagcg
     3841 ttctgaagat gagatcgagt ggtagttacg atgtgcagtc aacggtaccg gaggcggttt
     3901 gtgccgagga taaggagttg acggctaaac cactagccta ttaagctgac atttgctaaa
     3961 aaatggcaag tccatttttt ggccaactta gcgttagcga agtttggggg cgcccttttt
     4021 aaagggtgcg gttttatgtt tgcatgacta agaatgagtg ggaaaattta acagccctat
     4081 ctaacaggcg gttaaatgta caaaatatgt acaccccctt aaaaatgcag tggtcgattg
     4141 atcgaatttt atgtctttgg gttttggggt tttaaaacga aaaaaagggg gcgattttcg
     4201 ccctcttttt tgatgttttt tcttatcttg atactatagg caactatttt cggcagaaaa
     4261 tcggggctat agtatgggtc tatcccttgt ggctctaagg aagaac
//
LOCUS       DAFFFQ010000138         4199 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000138
VERSION     DAFFFQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.143"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(2..1222)
                     /locus_tag="LQB69_002850"
     CDS             complement(2..1222)
                     /locus_tag="LQB69_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBL3526953.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1853)
                     /locus_tag="LQB69_002851"
     CDS             complement(1242..1853)
                     /locus_tag="LQB69_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL3526954.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="LQB69_002852"
     CDS             complement(1896..2828)
                     /locus_tag="LQB69_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL3526955.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3253..4110)
                     /locus_tag="LQB69_002853"
     CDS             complement(3253..4110)
                     /locus_tag="LQB69_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526956.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctttaatt ggttaagat
//
LOCUS       DAFFFQ010000139         4174 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000139
VERSION     DAFFFQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.144"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            612..1424
                     /locus_tag="LQB69_002854"
     CDS             612..1424
                     /locus_tag="LQB69_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526957.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="LQB69_002855"
     CDS             3059..3613
                     /locus_tag="LQB69_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3526958.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4174
                     /locus_tag="LQB69_002856"
     CDS             3867..>4174
                     /locus_tag="LQB69_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526959.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     "
BASE COUNT         1542 a          516 c          660 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gttt
//
LOCUS       DAFFFQ010000140         4112 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000140
VERSION     DAFFFQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.145"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(39..281)
                     /locus_tag="LQB69_002857"
     CDS             complement(39..281)
                     /locus_tag="LQB69_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL3526960.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(313..870)
                     /locus_tag="LQB69_002858"
     CDS             complement(313..870)
                     /locus_tag="LQB69_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL3526961.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(883..1071)
                     /locus_tag="LQB69_002859"
     CDS             complement(883..1071)
                     /locus_tag="LQB69_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL3526962.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LQB69_002860"
     CDS             complement(1084..1650)
                     /gene="amaP"
                     /locus_tag="LQB69_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL3526963.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            2067..2240
                     /locus_tag="LQB69_002861"
     CDS             2067..2240
                     /locus_tag="LQB69_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526964.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            2880..3353
                     /locus_tag="LQB69_002862"
     CDS             2880..3353
                     /locus_tag="LQB69_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL3526965.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3362..3589
                     /locus_tag="LQB69_002863"
     CDS             3362..3589
                     /locus_tag="LQB69_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3526966.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1234 a          740 c          617 g         1521 t
ORIGIN      
        1 ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg tgcaaagaat
       61 gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc cattcctgca
      121 attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac taaaccagca
      181 atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc tgcaattgct
      241 ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc ctcctaactg
      301 acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt gtgcaccttt
      361 gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg tggattttgc
      421 tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt cttgtgtttt
      481 gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat tgtttacttc
      541 agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac cgtattctac
      601 gatcacatct agatccacag ctacttgttt cttacctact tctgtattga tacctgctgt
      661 tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc catctaatgt
      721 caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga ctttgtcttc
      781 ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca aaactgatcc
      841 atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt taaaaaattg
      901 atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg ctgtcatgat
      961 tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta gcagtagtcc
     1021 aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca ttatttggcc
     1081 ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt cataaccgct
     1141 gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta cttcttcttc
     1201 gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc
     1261 gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag gtgaatgaat
     1321 gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt tgtcgattgt
     1381 tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat agaaaagaac
     1441 gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga aaatatattg
     1501 ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt caggcataat
     1561 atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta aactcatgat
     1621 aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt atttttaccc
     1681 tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat agcatcagta
     1741 aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag tctgatactg
     1801 aaataacaat tgcttactta ttctctattt acttttgatt gctggctata cttaaaggat
     1861 attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag attctattta
     1921 tttattaaat tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc
     1981 gttcttactt tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc
     2041 ttataaataa aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa
     2101 cgattggcta tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat
     2161 tcagttggac tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac
     2221 aaaattatcg aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta
     2281 tggtactttt tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac
     2341 ttttgattta taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga
     2401 tagtaccacc ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata
     2461 aaggacaaat aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa
     2521 taaacatgaa cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct
     2581 catacagttc tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta
     2641 ttgtccaata taatcccagg catctaaact agcattatag aagagacaca catagaatct
     2701 aaaataactg gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac
     2761 cttcatactc tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag
     2821 ttgttttaca ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa
     2881 tgaaatttaa aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt
     2941 tgttcggcgt acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt
     3001 ggcagaccac attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg
     3061 cttatttctt attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa
     3121 gcttaaacat actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg
     3181 gcttacttcc taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg
     3241 taggaatcgc tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa
     3301 aactgcttga acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga
     3361 catggcaatt atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga
     3421 attgagtgag aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc
     3481 aaaagccatc cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg
     3541 tgatttgttg acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga
     3601 acaaacagta aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt
     3661 acgtctatta ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat
     3721 gtactgactc atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca
     3781 actggtttcc tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca
     3841 ccagactgat agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt
     3901 tcataccttg aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat
     3961 tcttttttag taagctccca tatatactgt aaagccttct atatattatg tcatgttgct
     4021 ttttaacatt tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct
     4081 tttttatttt taacattatt ttgataataa tg
//
LOCUS       DAFFFQ010000141         4074 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000141
VERSION     DAFFFQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.147"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(77..1051)
                     /locus_tag="LQB69_002864"
     CDS             complement(77..1051)
                     /locus_tag="LQB69_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3526967.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVYESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="LQB69_002865"
     CDS             complement(1500..2915)
                     /locus_tag="LQB69_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL3526968.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESISAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLNSFEKLSDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDVSA
                     VVAENKKLKEENRSLRRRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="LQB69_002866"
     CDS             complement(3137..3499)
                     /locus_tag="LQB69_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526969.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKH
                     FGFESFDDFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPNESDGLLGVIPSKK
                     SQMSESKFTSMFNDVSLR"
     gene            complement(3503..3850)
                     /locus_tag="LQB69_002867"
     CDS             complement(3503..3850)
                     /locus_tag="LQB69_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526970.1"
                     /translation="MVAVLEMRCNMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERMMDVLNSTINVGLANSSLITDKFVYGKIAETEDESEKEFWLNILTYVMQLNQI
                     GVIYRNKSKGKEL"
     gene            complement(3921..4061)
                     /locus_tag="LQB69_002868"
     CDS             complement(3921..4061)
                     /locus_tag="LQB69_002868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526971.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1153 a          896 c          576 g         1449 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatctaag ctttcataaa
      361 ccatagcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgcc gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacacat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgataaga accatctgat aatttttcaa aactattgag tggcacagga
     1981 tctttttgaa atacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaatg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat atgcttatgc
     2821 caatcattgg ttttctggct atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaactca cgtcattaaa cattgacgta aatttactct
     3181 cactcatctg acttttttta ctcggtatca ctccgagtaa gccgtcagat tcattgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcatca aaagattcaa
     3361 atccaaaatg cttccgaagt gccttaccca tttcagcatt ttccttctca atcaatcgtt
     3421 ttttctgttg acgaagtttt gcctgttctt ttttcaattt ttcttcttgt gctttaatat
     3481 ctgctaaagt cattcccaag atttacagct ccttgccttt tgatttattt cgataaataa
     3541 caccaatttg atttaattgc ataacataag ttaaaatatt cagccaaaat tccttttcag
     3601 attcatcttc tgtttcggct attttaccgt aaacaaattt atcagtaatc aaagaagaat
     3661 ttgctaggcc tacattgatt gtagaattga gaacgtccat cattctctca agcatttcac
     3721 tataattatc ttcatccggc aaattcaaat aatcagctaa tcgttcatac atcaattcaa
     3781 ttctatcaac tgtctcttct cttaaatcaa ttttatccat gttacacctc atttccaaaa
     3841 ctgccaccat ttccgattct ttaattttcg atacacctaa ttcatcagca agatttttta
     3901 tggtcatctt ttctgacaaa tcagtcatta gcttctccac tactttcagc taccgcataa
     3961 atatatttaa cggcgtgctt agggataata atcggagagt caattttatt atttttcaaa
     4021 atcaattcat attgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DAFFFQ010000142         3956 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000142
VERSION     DAFFFQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.148"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..156
                     /locus_tag="LQB69_002869"
     CDS             <1..156
                     /locus_tag="LQB69_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL3526972.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="LQB69_002870"
     CDS             175..1452
                     /locus_tag="LQB69_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526973.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="LQB69_002871"
     CDS             1462..2118
                     /locus_tag="LQB69_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL3526974.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="LQB69_002872"
     CDS             2118..3494
                     /locus_tag="LQB69_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL3526975.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="LQB69_002873"
     CDS             3624..>3956
                     /locus_tag="LQB69_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3526976.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DAFFFQ010000143         3923 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000143
VERSION     DAFFFQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.149"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(573..1130)
                     /locus_tag="LQB69_002874"
     CDS             complement(573..1130)
                     /locus_tag="LQB69_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526977.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="LQB69_002875"
     CDS             complement(1309..1920)
                     /locus_tag="LQB69_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526978.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSARYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="LQB69_002876"
     CDS             complement(2033..2290)
                     /locus_tag="LQB69_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526979.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="LQB69_002877"
     CDS             complement(2887..3165)
                     /locus_tag="LQB69_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526980.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="LQB69_002878"
     CDS             complement(3199..3450)
                     /locus_tag="LQB69_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526981.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          800 c          520 g         1409 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atatcgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAFFFQ010000144         3739 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000144
VERSION     DAFFFQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.150"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            11..478
                     /locus_tag="LQB69_002879"
     CDS             11..478
                     /locus_tag="LQB69_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526982.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
     gene            683..2347
                     /gene="alsS"
                     /locus_tag="LQB69_002880"
     CDS             683..2347
                     /gene="alsS"
                     /locus_tag="LQB69_002880"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL3526983.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            2360..3070
                     /gene="budA"
                     /locus_tag="LQB69_002881"
     CDS             2360..3070
                     /gene="budA"
                     /locus_tag="LQB69_002881"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL3526984.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            3200..3697
                     /locus_tag="LQB69_002882"
     CDS             3200..3697
                     /locus_tag="LQB69_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL3526985.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1233 a          596 c          807 g         1103 t
ORIGIN      
        1 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
       61 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
      121 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
      181 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
      241 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
      301 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
      361 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
      421 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
      481 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
      541 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
      601 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
      661 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
      721 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
      781 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
      841 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
      901 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
      961 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
     1021 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
     1081 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
     1141 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
     1201 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
     1261 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
     1321 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
     1381 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
     1441 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
     1501 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
     1561 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
     1621 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
     1681 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
     1741 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
     1801 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
     1861 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
     1921 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
     1981 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
     2041 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
     2101 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
     2161 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
     2221 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
     2281 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
     2341 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
     2401 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
     2461 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
     2521 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
     2581 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
     2641 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
     2701 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
     2761 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
     2821 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
     2881 tcacttgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
     2941 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
     3001 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
     3061 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
     3121 ttttgaggag gaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
     3181 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
     3241 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
     3301 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
     3361 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
     3421 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
     3481 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
     3541 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
     3601 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
     3661 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
     3721 aagagaaatc aagaataaa
//
LOCUS       DAFFFQ010000145         3589 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000145
VERSION     DAFFFQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.151"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..525
                     /locus_tag="LQB69_002883"
     CDS             <1..525
                     /locus_tag="LQB69_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL3526986.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2055
                     /locus_tag="LQB69_002884"
     CDS             586..2055
                     /locus_tag="LQB69_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL3526987.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2066..>3589
                     /locus_tag="LQB69_002885"
     CDS             2066..>3589
                     /locus_tag="LQB69_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3526988.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     "
BASE COUNT         1325 a          613 c          787 g          864 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca
      841 gccacagatt ttattctaaa gaatgaagac aaaacagaac gagtagaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
     1081 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
     1141 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
     1201 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
     1261 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
     1321 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
     1381 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
     1441 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
     1501 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
     1561 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
     1621 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1681 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1741 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1801 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1861 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1921 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1981 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     2041 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     2101 agcgaaggct tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat
     2161 ccaagatcga ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga
     2221 gttagattcg cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc
     2281 tattttcccc aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt
     2341 cgtcaaacga cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga
     2401 aggggagctg attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt
     2461 taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt
     2521 acgagacgca gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga
     2581 ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat
     2641 accaggaagt ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt
     2701 gcaggaaaag attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat
     2761 cgctcaaaat ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga
     2821 agcatttgaa gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat
     2881 ccttcaagtc gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag
     2941 ccttcagtct aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat
     3001 gcaagggttg tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac
     3061 ggaaggggaa tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc
     3121 cgaagcgatt ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt
     3181 ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca
     3241 actaagtcca ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt
     3301 tgcggaaaag tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt
     3361 aaaggcaatc gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag
     3421 cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt
     3481 gacggtggat ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg
     3541 tacgttgatc actgcaatga aaacagctgg gaaaaccgtg gatagtgaa
//
LOCUS       DAFFFQ010000146         3580 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000146
VERSION     DAFFFQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.152"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..525
                     /locus_tag="LQB69_002886"
     CDS             <1..525
                     /locus_tag="LQB69_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526989.1"
                     /translation="LEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2046
                     /locus_tag="LQB69_002887"
     CDS             586..2046
                     /locus_tag="LQB69_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL3526990.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGLAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQGIGGDIIELTQLFFIDDSIQNPKQRFKAALDYIIKDENKVERVEN
                     LHFETQEYKHHPIDYRPLTEKGRSYLKEQRKFPEWLIDYGEQQGLILEMKPRNERKNY
                     IVQDDRLDYAIVFPWKDRHTDQILGATYQGTIVDYERFGKRGVDKRIDINPTPNHGFN
                     VLLGSPKNLKFFESPIDLLSYAALNRETIKDTWLVSMDGLKDAVVGHYVREAVTELAP
                     KEEFPESIEICVDNDRAGHVFWDKVHRLGVSNPFTGKAVYFEPNLPNDWQVPREYQTI
                     YEEVGKEMRVAPEAIMAIHKIENNLTDTNQIVSFGEIQGVFAKKLAPKEKAQSIVVKE
                     KCIEAAQQLKSCEKSDGTYDFDRFYRGKGKINEQIQFSLKAKHYFEGYKNHEHEFVSE
                     VKKDWNDQLKHEIQQQAIRKQKRAMLFQQGRQQERE"
     gene            2057..>3580
                     /locus_tag="LQB69_002888"
     CDS             2057..>3580
                     /locus_tag="LQB69_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL3526991.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDEKWKQWSLAHLPIFPNQYHYHVPKDKKKQFNVVKRQLQSADT
                     VVIATDSDREGELIAWSIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAHVITQNGAFDAKLDPNETQATQEAFEAYLKDKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEEKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPVDLGWKKLFGK
                     ESDGKEKEEEPLLPKVAKGEMVTVDLQVLEKETKPPKPYTEGTLITAMKTAGKTVDSE
                     "
BASE COUNT         1286 a          613 c          802 g          879 t
ORIGIN      
        1 ttggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtg
      241 cctgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt
      361 ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatt cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatggt
      661 ttagcggtag tcaataaagg acgagactat cgtttagaag accatgattc ttttgttttc
      721 gatcgaagaa aacagcgatt ttattggaac agtcaaggta ttggtgggga tattattgaa
      781 ttgactcaat tgttttttat tgacgattct attcaaaacc caaagcaacg ttttaaagca
      841 gcgctagatt atattataaa ggacgaaaat aaggtggaac gggtggaaaa tcttcacttt
      901 gaaacacaag aatataagca ccacccaatt gattatcgac cattgacaga aaaaggacga
      961 agttacttaa aagaacaacg aaaattccct gaatggttga ttgattacgg agaacaacaa
     1021 gggttgatcc tggaaatgaa gccacgcaat gaacgaaaaa attatattgt gcaagatgat
     1081 cgcttagact atgcgatcgt ttttccctgg aaagaccgtc atacagacca aatattaggg
     1141 gcaacttatc aagggactat cgtagactat gaacgttttg gaaaacgggg tgttgacaaa
     1201 cgaatagata taaatcctac gcctaatcac gggtttaatg ttcttcttgg atctcctaag
     1261 aatctgaaat tttttgagag tcccattgat ttacttagtt acgctgcttt aaatcgcgag
     1321 acgatcaaag acacgtggct tgtttctatg gatggactga aggatgcagt agtggggcac
     1381 tatgttcgag aagcggtgac agaattggcg ccgaaagaag aatttccaga atcgattgag
     1441 atttgtgtgg ataatgatcg agctggacac gtattctggg ataaggtaca ccgacttggt
     1501 gtgtcgaatc catttacagg aaaagcggtg tattttgaac cgaatcttcc caacgattgg
     1561 caagtaccga gagaatatca aacaatctat gaagaagtag ggaaagaaat gagagttgcg
     1621 cctgaagcaa tcatggctat tcataaaatc gaaaacaacc taacggatac taaccaaatc
     1681 gtatcgttcg gtgaaatcca gggagtattt gccaaaaaac ttgcgccaaa agagaaagcc
     1741 caatctattg ttgtaaagga aaaatgtata gaagcagctc agcagctaaa atcgtgtgaa
     1801 aaatctgatg gaacatacga ttttgatcgt ttttatcgtg gaaaagggaa aattaatgaa
     1861 caaattcaat tttcattgaa ggccaaacac tattttgaag gttataaaaa tcatgaacac
     1921 gaatttgttt ctgaagtgaa aaaagattgg aatgatcaat tgaagcatga gatccagcaa
     1981 caagcaatca gaaagcaaaa acgtgcgatg ttgtttcaac aaggcagaca acaagaaagg
     2041 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aaaccgtcac aagcgaaggc
     2101 ttatgccgat agcttttcta aagctacccg aaaagacggg tattttgaaa tccaagatcg
     2161 gttgtttcca ggagaaactg tgatcacgta tggttttggt catttagtcg agttagattc
     2221 gcccgatatg tacgatgaaa aatggaaaca atggtcatta gcgcatttgc ctatttttcc
     2281 aaatcaatac cactatcacg ttcctaaaga taagaaaaag cagtttaatg tcgtcaaacg
     2341 ccagctgcag tcagctgata cagttgtgat cgcgactgat agcgatcgtg aaggggagct
     2401 gattgcctgg tcaatcattc aacaagcagg cgccgatcaa ggaaaaacct ttaaaagact
     2461 ttggatcaac tccttagaaa aagaagccat ttatcaggga ttccaacagt tacgagacgc
     2521 agaagagacc tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat
     2581 tgggatgaat ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag
     2641 tttttcctta ggcagagtcc aaaccccaac gctctacatg atttatcaat tgcaggaaaa
     2701 gattagaaac ttccaaaaag aaccatattt tgaaggaaaa gctcatgtaa tcactcaaaa
     2761 tggcgcattt gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga
     2821 agcttattta aaagacaaag gtgttcaatt aggaaaacag ccaggtacga tccttcaagt
     2881 cgaaacagag aaaaaaagtg cggctagccc acgtcttttt tctttatcga gccttcagtc
     2941 taaaatgaat caactcatga aagctagtgc caaagatact ttggaagcca tgcaagggtt
     3001 gtatgaagag aaatatctaa gctatccgcg aacggatacc ccttatatta cggaagggga
     3061 atatgcgtat ctgttggatc acttagacga atacaagcat tttttgaggg ctgaagaaat
     3121 tccaacccct attcatacgc cgaatagtcg gtatgtgaac aataagaaag tccaggaaca
     3181 ttacgcgatc attcctacaa aaacggttat gacagcagcg gcttttgaac aattaagtcc
     3241 actgcagcag gctatttatg aacaagtgtt aaaaacaacg gtggccatgt ttgcggaaaa
     3301 gtatacctat gaagaaacaa cgatcttgac gcaagttcag cagcttcagt taaaagcaat
     3361 cggaaaagtg ccggtagatc taggttggaa gaagttattt ggaaaagaga gcgacggaaa
     3421 agagaaggaa gaagagccgc tacttcctaa agtggcaaaa ggagaaatgg tgacggtaga
     3481 tctacaagtt cttgaaaaag aaaccaagcc accaaaacca tataccgaag gtacgttgat
     3541 cacggcgatg aaaacagctg ggaaaaccgt ggatagtgaa
//
LOCUS       DAFFFQ010000147         3533 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000147
VERSION     DAFFFQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.153"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..174
                     /locus_tag="LQB69_002889"
     CDS             <1..174
                     /locus_tag="LQB69_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526992.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            167..307
                     /locus_tag="LQB69_002890"
     CDS             167..307
                     /locus_tag="LQB69_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL3526993.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="LQB69_002891"
     CDS             377..796
                     /locus_tag="LQB69_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL3526994.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="LQB69_002892"
     CDS             793..1803
                     /locus_tag="LQB69_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3526995.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..3126
                     /locus_tag="LQB69_002893"
     CDS             1918..3126
                     /locus_tag="LQB69_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526996.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            3110..3280
                     /locus_tag="LQB69_002894"
     CDS             3110..3280
                     /locus_tag="LQB69_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3526997.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         1293 a          507 c          668 g         1065 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
     2461 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
     2521 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
     2581 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
     2641 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
     2701 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
     2761 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
     2821 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
     2881 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
     2941 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
     3001 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
     3061 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
     3121 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
     3181 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
     3241 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
     3301 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
     3361 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
     3421 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
     3481 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttc
//
LOCUS       DAFFFQ010000148         3460 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000148
VERSION     DAFFFQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.154"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LQB69_002895"
     CDS             complement(578..1594)
                     /gene="tsaD"
                     /locus_tag="LQB69_002895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL3526998.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LQB69_002896"
     CDS             complement(1591..2055)
                     /gene="rimI"
                     /locus_tag="LQB69_002896"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL3526999.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LQB69_002897"
     CDS             complement(2062..2604)
                     /gene="rimI"
                     /locus_tag="LQB69_002897"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL3527000.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LQB69_002898"
     CDS             complement(2588..3310)
                     /gene="tsaB"
                     /locus_tag="LQB69_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL3527001.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT          989 a          757 c          594 g         1120 t
ORIGIN      
        1 atatcagggt cactgcttat tttcctccct aaaacttctg aaagatccgg cagttttaga
       61 tcactggaca agactcaaag attttctatg tgtgtttcag ttatgtaata ttcactgttt
      121 gcaaaaaata ctgttagatt aactagatga agcgtcttgt cctcatccga tctagattat
      181 tcgatattcc tcgacaataa acgattactc agtgagggca atcagaggaa cggataatga
      241 taaataagct gtaaaaaata gcaaaataac aataatgatt aaaagtatgt aaaaaatagg
      301 gctttgacaa aaacgtgtca cagccctatt tcgattaaac ggtgtgtacc tgtcaactct
      361 tcggtcacaa gtcataatgt tcataaacat gaggagctgt tttttgaaat tgttccttgt
      421 gaatcagagt taaacgacag gcttacgaac tcttgattaa acggcgttca aaagcttgca
      481 tacgcggtaa taagaacagc caggtcaaaa acatgagaag ccgtttttcc tgggctgttc
      541 ttattacttg atgcagggca gctttttcac aacctcttta ttcagataaa accaagcttg
      601 gatcagcatt caactgatag ctagcaaaat ttcttttttg catttccaca tgtgctgctg
      661 ctccaatcat tgcagcatta tctccacaaa gtcgaagagg gggaatcact agttctacct
      721 cagggagttt agttgccaaa gcatcctgca atccttcacg caacccttga ttggccgcta
      781 cacctccagc tataatcagt tgcttgactg gataattttg acaagctcga agtgttttag
      841 taatcaatac atcgatcaca cttgcttgaa agctagctgc taaatcattt ttgtctagat
      901 cttctccacg ctgttgtgca ttatgcacca aattgataaa tgcacttttt aatccgctaa
      961 aactgaaatc ataattatct tcatggatca tcgctcgcgg aaaatggtaa ttatcttttc
     1021 cctgatgagc tagctgatcg atttcttttc cgcttgggta agaaagacct aatactcttc
     1081 caactttgtc ataagcttct cctgctgcgt catctcttgt ctccccaatg atttcatatg
     1141 atccatcttc ttgcatatag actaattctg tatgtcctcc gctaacaaga agtgccatca
     1201 atggaaactg gaatggttta accagacggg ctgcatagat atgccctgcc atatggttta
     1261 caggaataag cggcaattgg tgtgcccaag caaaagcctt agctgcagaa ataccaatca
     1321 gcaacgagcc gacaagtcct ggtccataag tgacagcgac tgccgataaa tcttctgccg
     1381 acacaccagc ttctaccagt gcatcttcca agcataaggt gatttgttct acgtgatggc
     1441 ggctagctac ttcaggtact acgccaccaa aacgtttatg actgtttatt tgagaagcaa
     1501 cgatgttaga taagatctct gttccatttc taacgaccgc tacactcgtt tcatcacaac
     1561 ttgattcaat cgccagaatc aattcttcat tcatttcatc gtctccgttt ttaactttct
     1621 gctcataatg atcgcatctt ccgtcggatt gcgataatat gatttccgtt tacctaatat
     1681 acgaaacttt tctgattcat aaagttttct tgctgcttca ttagatagac gtacttctaa
     1741 aaaaacatgc agcgaatctt ttatttgttc cgtaatcagt aattgtaaca aatgccgtgc
     1801 atgccctttt ccttgttctg aaactgcaac agcaatattt gtgatctcga cttcatctat
     1861 tactttcgag tatccaataa agccacagat ctggttatct tcagctaaaa gcagataatc
     1921 agagtgaggc tgttggatgt ctgccaaaaa ctgcattttc gtccaaggtg aaccatgtgg
     1981 ataagcattt tcgctaatca tccatacgca ttctgccaag tacgcttcat caaaatcttt
     2041 ttttctgcaa atcaactaat ctcactcatt ttctaaaagt tttatcatat ccaacgcatc
     2101 ttcttttgtt tcagtgtaat atcttttttt gatcgttgtt gcttgaaacc ccaatttccg
     2161 ataaagtctt tgtgcatctt gattactcat acgcacttct aatgacatcg tctcacagcg
     2221 attcattata gcaaagttct cgatttcgtc aataagcagc gaaccgattc gtttacgctg
     2281 atatgccggt cgaacggcaa tattcgtgat atggcaatct aaaccgaacg ctcttgaacc
     2341 gataaaacca atcattcttc cattatcaaa gatacctaga tatagatgag gaagcggtga
     2401 cttcaattct gatataaagg cactttttgt ccatggcaat tcgccattat agacttctcg
     2461 ttcgacatcc agcagctctt tgatatcttc tgtagtcaat tctcttagta cgtaacgacg
     2521 atcgatttgt tcgactgttt tttctgtata aatcatttta tcttgaaaaa agcttctggc
     2581 atacgctcta aattttttca acataacttt cgtctccagg tgtgtgcgtc tccaaccatt
     2641 tttcttctgc ttctactcgt tttaaatagc gaggaagaaa agcttgtttc tctgcaactg
     2701 gttcaactaa agcacctaat tgagccacag ttacaccact tggaatatcc catgctggaa
     2761 ctgtattaat catcgcacta ggcaatattt cctttatttc agagaagaat ttgtgacaat
     2821 cactcccgac gaaataaata gcttcattca gcttttgtag ttgtgtgaac aaatcagaag
     2881 cagaaatatg tacatcttca agtacagttt ctaatctatt ttctttccag cggtatgccc
     2941 cagcgtatac attttttctt cgagcatcaa acatgggcac aataatatta tccacaccga
     3001 tgcaattagc cgcgatggtt ttcagactag atactcctac tagctcttta tctaatgtat
     3061 aagcaatcgt ttttgctgtc gttacaccta tacgaagccc tgtataagaa ccgggaccat
     3121 cagaaaccgc gattcggtca atgtcttttg gacttatttg aagatctgca aacaattggt
     3181 cgattgcagg catcagtgtt acactgtggt tgcgtttgat atttgtttgt atttgtccaa
     3241 gaatttgttc gtcttctacc acaccgatcg ccaaagtttg gtttgcagta tctattccta
     3301 atgtaatcat atatttgatc ctttctgcta tgacattctc gccttccatt gtagcatact
     3361 tttttattct gaggatgaaa ataccaaagg caaaactagt acaataatac atgattatct
     3421 gctggcgggc aaattttgcc ccttcctgaa acacaaaaaa
//
LOCUS       DAFFFQ010000149         3449 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000149
VERSION     DAFFFQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.155"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            116..976
                     /locus_tag="LQB69_002899"
                     /pseudo
     CDS             116..976
                     /locus_tag="LQB69_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="LQB69_002900"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="LQB69_002900"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBL3527002.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="LQB69_002901"
     CDS             1830..2678
                     /locus_tag="LQB69_002901"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBL3527003.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="LQB69_002902"
     CDS             2688..3083
                     /locus_tag="LQB69_002902"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBL3527004.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..>3449
                     /locus_tag="LQB69_002903"
     CDS             3285..>3449
                     /locus_tag="LQB69_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527005.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT         1336 a          374 c          687 g         1052 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaa
//
LOCUS       DAFFFQ010000150         3402 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000150
VERSION     DAFFFQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.156"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(75..1073)
                     /locus_tag="LQB69_002904"
     CDS             complement(75..1073)
                     /locus_tag="LQB69_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL3527006.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="LQB69_002905"
     CDS             complement(1148..1444)
                     /locus_tag="LQB69_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL3527007.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="LQB69_002906"
     CDS             complement(1437..1730)
                     /locus_tag="LQB69_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527008.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="LQB69_002907"
     CDS             complement(1876..2427)
                     /locus_tag="LQB69_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL3527009.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="LQB69_002908"
     CDS             complement(2601..2900)
                     /locus_tag="LQB69_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL3527010.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEIGTLPKDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3235)
                     /locus_tag="LQB69_002909"
     CDS             complement(2927..3235)
                     /locus_tag="LQB69_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL3527011.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQLPLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY
                     "
BASE COUNT          990 a          664 c          464 g         1284 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttt aggaagtgta cctatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaagttgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga aacatttctt
     3241 ttccctcctt tgctataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa at
//
LOCUS       DAFFFQ010000151         3398 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000151
VERSION     DAFFFQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.157"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..276
                     /locus_tag="LQB69_002910"
     CDS             <1..276
                     /locus_tag="LQB69_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBL3527012.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="LQB69_002911"
     CDS             419..640
                     /locus_tag="LQB69_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3527013.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="LQB69_002912"
     CDS             656..1498
                     /locus_tag="LQB69_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527014.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="LQB69_002913"
     CDS             1561..3363
                     /locus_tag="LQB69_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBL3527015.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1228 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actgctggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DAFFFQ010000152         3376 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000152
VERSION     DAFFFQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.158"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            37..588
                     /locus_tag="LQB69_002914"
     CDS             37..588
                     /locus_tag="LQB69_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HBL3527016.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1518
                     /locus_tag="LQB69_002915"
                     /pseudo
     CDS             1264..1518
                     /locus_tag="LQB69_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1588..2100
                     /locus_tag="LQB69_002916"
     CDS             1588..2100
                     /locus_tag="LQB69_002916"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527017.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="LQB69_002917"
     CDS             2097..3134
                     /locus_tag="LQB69_002917"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3527018.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3294..3376
                     /locus_tag="LQB69_002918"
                     /pseudo
     CDS             3294..3376
                     /locus_tag="LQB69_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1242 a          576 c          669 g          889 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcatcttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac accgaccatc
     3301 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct acaacgatta
     3361 aaggcatgga agccat
//
LOCUS       DAFFFQ010000153         3283 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000153
VERSION     DAFFFQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.159"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            3..587
                     /locus_tag="LQB69_002919"
     CDS             3..587
                     /locus_tag="LQB69_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL3527019.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(664..1857)
                     /locus_tag="LQB69_002920"
     CDS             complement(664..1857)
                     /locus_tag="LQB69_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL3527020.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1921..2301
                     /locus_tag="LQB69_002921"
     CDS             1921..2301
                     /locus_tag="LQB69_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL3527021.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2375..2737
                     /locus_tag="LQB69_002922"
     CDS             2375..2737
                     /locus_tag="LQB69_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527022.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1114 a          538 c          623 g         1008 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 gatttaatcg attgtctcta aagcatactt gattcgatta agtccttcca gtaaagtttc
      721 ttctggacaa gcaatattca agcgcatatg cccatgaccg gaaggaccaa acgtgatgcc
      781 aggatttaat acgacttttc ctttccgaac aagagtgttc tccaattctt ggtcgtctga
      841 aatataggcg gtaaaatcta accacattaa gtaagttcct tcagggttca ttattctcac
      901 tttcggaaga tactgttcaa aaaactgatt aactgtttga acattttttt ctagataagg
      961 aatcagttgg cttaaccatt ctccacccgt ctcataagcc gcttgggttc caatcagtcc
     1021 aaaagtgttg atttcgtgct gttggttcat cactaaatgt ttttcaaaat cagctttcat
     1081 ctctggattt ttgataaata ccattgaatt tttgattgca gctagattga aagtcttggt
     1141 tgcggaagta aaagcaacta gtagatcatc taaattagga tcaatcgtta gcatggaagt
     1201 aaatgtatga tcgaataaag ttaaatcttg atggatctcg tcactaaaca gaatcacttg
     1261 atgttttaaa cacaattcgc ttaactgttt caattcttct ttgctccaaa cacgccctcc
     1321 aggattgtga ggattgcata aaatcattgc ttttacattg ttttcttcga tttttttctc
     1381 catgtcagac aagtccatga tgaaatgatt atttttttct agcaaagagc ttcgaacgat
     1441 gtgacgttga tttgtttcta taatagagga aaatggagga taaactgggt catgaatcaa
     1501 aatagaatcg cctggttttg taaaagtttg tactgctgca gctaaactgg ttaacacacc
     1561 acttgtaaaa acgatatttt ctttcgtcaa ctccacagca tggtgttggc gttcccaatg
     1621 aatgatagcc tgatatagtt tatcagacag agtggcataa ccaaagattc catgtttgat
     1681 gtattcacta aacgctttag ctaccccacc gggagacaag aagtccatat ctgctaccca
     1741 caaaggaagt aagtcatcca tttgatatgt ctcagcaatt gcgtcccatt ttacactttc
     1801 tgtatctttt cttgaaatct gtttatcaaa ttcgatcatc ttaagacctc cagtcatttt
     1861 cgtcgcttct ttttttgagc tatttacagt ataatgataa atgcgaagag agggaagtag
     1921 atgacttata aaattgggca aataattgaa ggaaaagtga cagggataca gccttatggc
     1981 gcattcgttt ctcttgataa cgagacgcaa ggacttattc atgtttcaga agtacaatcc
     2041 ggttatacga aaaatatcca ttcattgtta aaagtcggac agccagtaac agttcagatc
     2101 atcgacattg atgaatattc caaaaaaatc agtttatctt taagaacgtt agaaaaaaca
     2161 aatccagctg ttccatatcg cagaaaaagg tatttcacga ataaaaatcg taagatcgga
     2221 tttaccacta ttgcggagca gttacctatt tggatagaag aagcattatc tgaactagaa
     2281 cggaaagaaa ataagaaata aaaagtgtat tttttctgaa aatgtgctag aatttttatg
     2341 taagccatag aacaagttga tcgaggtgga aattttggca agcaaaagag ttgctggtac
     2401 aattatgttg catttggagg atggtactaa aaaatttttg gtccattcaa ttgatgacaa
     2461 gttggaattt gcgttagcgg aactttctga aggaaagact ggtttagcga atatacttaa
     2521 tttcttaaaa gaaatagttc atatcgatgt tagcaagatt agtttgatgg aattgacaaa
     2581 tacacatatt aaagaggaaa atgttccgtt gtttgtcttt gaaactgaac aaaagaatca
     2641 aagagaagaa ttaggcgaag gatatatttg ggaagaacca ggtcaaatgc gctctgttct
     2701 tggaacttac gaagtagagg gagtgccttt cttctaaaat acagattaag cgaacaaaga
     2761 agtttgaatt tttttcaaac ttcttttttt attgtttggt ccaaaaaata caatgaaatg
     2821 ttctgctaaa gatagttttt tagtgcattt tattttcaaa atatgttgaa aacggttgta
     2881 taacgaataa taataattga tcgaaaaatc atttcacaaa gtaatgctta atttatagtt
     2941 gaccaaggaa gaaatacatg ataaatttaa cagcgttgat tctgaagtgg atgttatatc
     3001 acatttctaa aaaatttaaa aaatagttat tgacatgtta ctaacgacat gataagataa
     3061 ctgagtcgtt aaaaaagaac cacttgcgaa gcttgattct atcgaaaaaa acttcaaaaa
     3121 agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa gatttcgaaa
     3181 aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gcttgtctct ctgatatgat
     3241 ataaaagttg ctacggcagc caaaaacgaa tcgggaaaca ctc
//
LOCUS       DAFFFQ010000154         3279 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000154
VERSION     DAFFFQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.160"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(366..1484)
                     /locus_tag="LQB69_002923"
     CDS             complement(366..1484)
                     /locus_tag="LQB69_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527023.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="LQB69_002924"
     CDS             1955..3262
                     /locus_tag="LQB69_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL3527024.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         1032 a          679 c          590 g          978 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaa
//
LOCUS       DAFFFQ010000155         3218 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000155
VERSION     DAFFFQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.161"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            50..1438
                     /locus_tag="LQB69_002925"
     CDS             50..1438
                     /locus_tag="LQB69_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBL3527025.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3218
                     /locus_tag="LQB69_002926"
     CDS             1776..>3218
                     /locus_tag="LQB69_002926"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL3527026.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaa
//
LOCUS       DAFFFQ010000156         3177 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.162, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000156
VERSION     DAFFFQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.162"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(7..978)
                     /locus_tag="LQB69_002927"
     CDS             complement(7..978)
                     /locus_tag="LQB69_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL3527027.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            1684..3063
                     /gene="brnQ"
                     /locus_tag="LQB69_002928"
     CDS             1684..3063
                     /gene="brnQ"
                     /locus_tag="LQB69_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL3527028.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT          892 a          634 c          593 g         1058 t
ORIGIN      
        1 caaagcttat tcttgatgga gatctgcctt ttcataagct ttgatgattt cggcaactac
       61 agggtgccga acaacgtcac ttgcttcaaa attaacaaaa tcgattttct tgatttgctt
      121 taatgttcgt tctgcatgaa caagaccact tgttgtccct cgaggcagat caatctgact
      181 cgtatctcca ttgacaatca tttttgattg ataaccaaga cgtgtcagga acattttcat
      241 ctgagcaact gtcgtgttct gtgcttcatc caaaataacg aatgcatcat ccaatgtccg
      301 cccccgcatg taagctaaag gagcaatttc gatcacgcct cgttccatca atcggttcgt
      361 atgatctaat ccaaaaattt ggtataaagc atcataaaca ggacgcagat aaggatctac
      421 tttttctttt agatccccgg gaagaaagcc tagattttcc cctgcttcca cagcaggccg
      481 cgtcaaaatg attttttgca cttcaccttt tttcaaggca gcgattgcta atactactgc
      541 aagaaaagtt tttcctgtcc cggctggtcc tacgccaaat acgatatcat gtttggctac
      601 cgattcgaca tatttctttt ggccgctgtt ttttacccgt attggttttc cattacggtc
      661 tttgacgatc tcttcctcat acatttcaac aaaataatct aatgtgcctt tttgccccat
      721 tttcagtgcc gtcacgacat ctggtgtact tacatggatt cctcgtttga tcaattcctg
      781 caatgtccga atgactgagg cagccaagga aatctgggat tgttcgccaa tgatctgaat
      841 gatttctcca cgtgtatgga tggttgtttc tgttgtttct tcaatcagtt ttatatgctt
      901 gtcgtgtgca cctaaaagca tgctgacatc atcagcagcc gtcaatctaa tctctaaaga
      961 actagactct tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt
     1021 accatactca gaagaaacta tatagcaaat aagaaaaggt tctaaaagag ttgcttaacc
     1081 ataagtacca agaaacgtta aaaaatagct gcttccattc tctaaaggtc tcgtttgaat
     1141 aaaaaaagct gatcatgtaa gctatgcata agatagagga caagtattcc gtctaactta
     1201 gcaagtattc tctgggtatt tttcttgttt caaaaaaaga aaacaaaata tttgaaatat
     1261 tcttgcattt caaaaaaaac aggtgtatta taacgtcaac aaataaatat acagcaaagc
     1321 gtcgaggaaa acagtaggct attcagcgat ttattcccag tgagtcatgt aagtgagaaa
     1381 tgacaataaa tcgaatagac gaacatcatt tccgagtcaa tgtcgaaaac gtagatcatt
     1441 gtggctgcct ccattatcag gctagcggat aactttatcg aaaagcgtcc gtgaatgaag
     1501 aagctttttt aagcgaaaaa aggtggtacc gttctagaat ctagagccct tttcccaagt
     1561 ggaaaagggc ttttttttat attttaaggt ggtggttttc atggaatctg atattagtgg
     1621 catttctagt ttctgcaacg tttattttgt tactcaaaat gataattatt ggaggaaaaa
     1681 tttatggaag aaacaaaaac ttattcatgg aagcaattat tgatggtcag ttcacttatc
     1741 ttcgggatgt tcttcgggtc aggtaatctg atctttcctg tccacttagg tcaaatggct
     1801 ggatcaaact ggatcagcgc tggtgtagga tttgccatct caggcgctct tttcccatta
     1861 ttagctatct tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct
     1921 gtcggaaaat ggtatgctgc cttgttcttg atccttgttc atttaacgat cggaccattc
     1981 tttggtacac cgcggacagc agcaactgct tttgaaatgg ctgctaaacc ctttttgcct
     2041 gaaaaatatg attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat
     2101 ttccttactg tacatccaac acgtttggtc aaatatgtcg gaaagtattt aaactccttg
     2161 tttcttgcat tattagctat catttttgtc gtttctttat tccaacccat ggggaatcta
     2221 aaccaatctg ctgcagattc ttatcagacg aacgctttga caaatggcgt tttagaagga
     2281 tacaacacgg ttgatgcagt agctttactt gctttaagtg tcacttttgt ccatgcagtt
     2341 cgtgatcttg gttttaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg
     2401 agcgttgcgt tagaagtcat gatttatttt ggtctggtcc ttctcggtgc gatgagtttg
     2461 aataagatgc ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat
     2521 ttaggtaact tcggtgctag cttcttaggg gtaatggtca cattaggtgt cttcactacc
     2581 gctatgggac tcgttgtttc ttttgcacaa gatttccata aactgttccc taaagtcagt
     2641 tatatgacat ggctgcggtt aacgactttt gtctcttttg tcgttgcgaa tgccggattg
     2701 gataatatca tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcacttgcg
     2761 ttaatcttgc tttccttaac agcaaaattc ttccaaaaga gtccatttgt ctatcaagta
     2821 acgatgttct tttctgccgt tccagccgta ttggatatgt tggcaaattc gcctgcatta
     2881 gtgagtcagc aaactgtcgt agcaagctta ctaggatttt atcatcatca cgttcctttt
     2941 gctgcattag gacttggctg gatggtccca acgttacttg gttacgctgg cagcttactc
     3001 ttttattacg cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa
     3061 taaccaaatc aaacacaatt taacataaga gatattaaac aggaaaaaaa cagctagaag
     3121 aaagaataag attctttctt ctagctgttt tttagttctt tttttacgaa cacaaaa
//
LOCUS       DAFFFQ010000157         3003 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000157
VERSION     DAFFFQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.163"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            178..882
                     /locus_tag="LQB69_002929"
     CDS             178..882
                     /locus_tag="LQB69_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL3527029.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            969..2258
                     /locus_tag="LQB69_002930"
     CDS             969..2258
                     /locus_tag="LQB69_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL3527030.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            2324..>3003
                     /locus_tag="LQB69_002931"
     CDS             2324..>3003
                     /locus_tag="LQB69_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3527031.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELR"
BASE COUNT         1220 a          399 c          515 g          869 t
ORIGIN      
        1 aactttctga ctaaattata tacttcggtt tttccgaagt caagtgcata tttcagtttt
       61 cctgaaatcg atttctattt tgcgtaaata cttctttaaa aacactaaaa tattttctaa
      121 gaaacctgtt acattttttc cgaaacggtg ttacaattaa ctcaaggagg tgttactatg
      181 ttcgctgaaa atctaaagtt tcttagagaa aagtataata ttgatcaaca agaactagcc
      241 gaaaaactag gaagaaaaag ttcatcctca ataagcgaat gggaaaaagg aaaatataca
      301 ccaaaattaa aaaccttaaa tgagatagct aagatattca atgttgatat agatgatttg
      361 atgaatattg atttatctat atctaatcag atatataata tccttcctat atataatcag
      421 cttaacgacg aaaataaagt tgctacctat gaatttgcaa gtaatcgatt agaagaacaa
      481 aaacaagaaa atgtcctaga ttttcctaaa aaagaaaaaa tcccaactgt tcacaattcg
      541 gcttcggctg ccaatccaac agaacttact tacggtgata cggttgtcga agaggaagaa
      601 tttgagagag ttccaagtgc tgctgacttc gcagtaccta ttataggcga ttcaatggaa
      661 cctgttatca ggaatggaca attcgtattc gttaaagaac aaccggacgt agaagatggg
      721 gaaattgcta ttgtggaatt aggtggtgac ggagttacat gtaaagagat atataaagat
      781 tacgaaaatc aaacaatcat tttacgttct ataaacgact tatacgaaga tagaattgta
      841 tcgccagaac agattagaat tatcggaaaa gttgtatttt aaaaaaattc ccatgccgga
      901 gccgtcaact tcggtaaggg tttatatgat tttgacaatt attttataaa agaagcgagg
      961 cgttttgaat gggagtacta ttgtttttag ttggaatagt tttaataatt atgggtagta
     1021 gcaaattatc tgaagctaaa aaattaaaag ataaaacttc tgccttagag agtaaggtaa
     1081 aggaatattc tcaagaatta aatgctaggg aaacgtttat atctgaaaaa gagaaaaaca
     1141 taagtaaatt gaataaaaac ttgtcaacgc tagaaaacaa aattgatatt cttaatgctg
     1201 aaagccagcg gttagacgat gaaattaatc aaaaatatga cacatctctg gtcactatct
     1261 ataaatcagt aattattccg aatatagatg aaacttcttc atccgaaata aaaaacaaac
     1321 tttctttatt aaagattaaa gaaaaggatt ttattttaga cggtgcaatt aatattcctt
     1381 cttatttgaa taatagagaa gctaataata ttaaaaagaa aatgcttata ccattagaat
     1441 cagaaataac tggacttctc aataaactta ctatttctaa cgtagattca actagagaaa
     1501 aaattataag aacttttgat aaaataaaca agctttttaa aaacgatgaa gcagaattca
     1561 aaaaagaatt acttgaatta aaacttcaag aattagaact caactattca tacatagtta
     1621 aagttaacga tgaaaaagaa cagcaaaaag caataaaaga acaaatgatt gaagaagaaa
     1681 aggtaagacg tgaaatagaa cgtgaaaaga aacgcattga taaagaagaa cgacagttta
     1741 actcagagat aactaagtta atggcataca tgcaaaaagc tagtgctgat gttgaaaaag
     1801 aactctatgc tgataaaatt aaagaactgg aagagaaatt aagagaacta gaaactgtca
     1861 aagaagatgt attgcaaaga gaacttaata ctagagctgg ctatgtttat gtcatcagta
     1921 atatcggatc tttcggtgaa gatgtctata aaataggaat gactagacgg ttagaaccta
     1981 tggatagaat taaggaactt agtagcgctt ctgtaccttt tgaatttgat gttcatgcaa
     2041 tgatattttc agaagatgca ccaacattag aaaatcaact acattcgcat tttagaaaaa
     2101 acgaagtcaa taaagttaat caacgaaaag aattctttaa agtatctctt gatgaaattg
     2161 aaaaagttgt tttagaaaat tataacggaa ctgtatcttt tactaaacta gctcaagctg
     2221 aacaatacag aagatcatta gaattaacag atgtgtagta aaaagattag ccttcgggct
     2281 tttcttttac aacgataaga acatacattc gaaaggagat aacatgaaac gagtagcatt
     2341 atatatgaga gtatcaactg aacaacaagc caaacatgga gatagtctta gagaacaaaa
     2401 agaaaccctc tatgaatata tcgaacaaca caaggatttg aaagtagtta atgaatatgt
     2461 agacggtggt atttctggtc aaaaaataaa tcgtgatgaa tttcaaaaac tattacaaga
     2521 tgtaaaagaa aataaaatag atctaatttt atttacaaaa ttagatagat ggtttagaaa
     2581 tctacgacat tatttaaaca ctcaagaaat tttagaaaaa cacaatgttt cttggaatgc
     2641 tgtttctcaa caatattatg atacaactac agcgtatggc agaactttca ttgcacaagt
     2701 aatgagtttt gctgaattgg aagcccaaat tgattcagaa cgcatcaaag ctgttatggc
     2761 aaataaaatt gcacaaggtg aagtagtaag cgggaaaact ccgttaggct actctataga
     2821 aaataagaaa ttagtaataa atgatgatgc tcctatcgtg atagatatat tcaactactt
     2881 tctttctagt ggtagtttaa gaaaaactgt ttattattta ggctctcagt atggaatcgt
     2941 tagagattat caaagtgtta aaaatatgtt gacgaacaaa aaatacattg gtgaattaag
     3001 aaa
//
LOCUS       DAFFFQ010000158         2985 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000158
VERSION     DAFFFQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.165"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            287..694
                     /locus_tag="LQB69_002932"
     CDS             287..694
                     /locus_tag="LQB69_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527032.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="LQB69_002933"
     CDS             708..1109
                     /locus_tag="LQB69_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527033.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="LQB69_002934"
     CDS             1111..1482
                     /locus_tag="LQB69_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527034.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="LQB69_002935"
     CDS             complement(1518..1820)
                     /locus_tag="LQB69_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527035.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1916..2710)
                     /locus_tag="LQB69_002936"
     CDS             complement(1916..2710)
                     /locus_tag="LQB69_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBL3527036.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
BASE COUNT         1052 a          424 c          458 g         1051 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat cgaatttaaa ccataattgt
     1801 gtttttcctt ccattaacat ataaagttca cctcaaaaag agtatacgaa caaatgtttg
     1861 tattgtaaat agaaaaagat gagctaaatt aaatgcccat cttctttgat agatattata
     1921 aatctaataa ttcttgtttc tttttattaa attcttcttc tgtaatgatt ccatcatcca
     1981 atagttcttt atattttctt atttctgaag ctgaggaaat tgttgatgta ttggaatttg
     2041 tagcagaaat atttcttctt tttaaattat aaataatttc gtttaaagaa tcacttatcc
     2101 tatcaacagt atctttatca actgcaacat tcaatacttt accaagagta tcaatagtta
     2161 atgtacccaa cagcactcct ctttttttac taatattatt taaattgtct aatataatac
     2221 tttgtacatt attgcctatc attttttgtt gagctacaat taaacgttta ttagttaatg
     2281 cgtatgcata attattgtca tgcttagttg atgagatata attatgcaat cctacaaagc
     2341 agaagattac gtattcgtca ctgttaagtt gatctgatat taagttaaaa tgatgagttg
     2401 tccatttttt agtcattcct ttaccgtagc cgtatttaag acaaaattct accatctctt
     2461 cagtggttct cattctatca ttacctatat catgattgtt ttgtgcagta tgatcagata
     2521 tgtttaattt tcttttttct gatggatcct tatctaaatc ttctaaattc aaagatctta
     2581 ttattttata aggagtagtc ataccgtaac cagctttttt catacaattg ccgcaaacaa
     2641 tttgtttatc tttgatttta tatgaagtta agccaatttt acctccgcac tttgcacatt
     2701 gtttactcac attagttcct cctagatatc agctttctac tactattaac attctatcaa
     2761 tagtgaccat actttgacca tactttttag aaagaataga aaattccaag atatttaata
     2821 atagaagaaa agcactaaca tcatgggttc agaagaattg agagcttatc aggaaacagt
     2881 ataattgaag tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa
     2941 gaggttgtga caagactttt gtcacagcct tttttgttta taaaa
//
LOCUS       DAFFFQ010000159         2953 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000159
VERSION     DAFFFQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.166"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            89..406
                     /locus_tag="LQB69_002937"
     CDS             89..406
                     /locus_tag="LQB69_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL3527037.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="LQB69_002938"
     CDS             complement(497..862)
                     /locus_tag="LQB69_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527038.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1069..1551
                     /locus_tag="LQB69_002939"
     CDS             1069..1551
                     /locus_tag="LQB69_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527039.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1748..2638
                     /locus_tag="LQB69_002940"
     CDS             1748..2638
                     /locus_tag="LQB69_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL3527040.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          919 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaagttttt ccccctaatt tttttagata
     1021 gaaaattgta tagtatgatg agagaaaaag tagtttgggg gaacgttaat gagaagaaaa
     1081 gcagtaatcg atcggcttta tttatttttt tggttttttc ctatcgtttt gctacctttt
     1141 acatgggtaa atgctgttgt acaaggtgcg gagatattag gcctgatcca aggaagcgga
     1201 tttgtggttt ctaggaattt tccagctttg attgttttct atttagtgat aagtgtaggt
     1261 ctcttattac caaaatctct gatcaatcac tacaattggc agctgcttta cttttcttgc
     1321 ttattagtat ttacatctac gttttctttg ctgcctcgct tgattatcgg ccctaatggt
     1381 ttgagcgaga gtggattgaa ggatttagct tattcctcca tgtatttctt gacgatcaaa
     1441 ccatggttct atttagtaat tgcctcattt attcttgcaa cattttttta cttctggaca
     1501 gaaatcagga taaaaagtga acgatctgta tctataaaaa ataactattg atggtggatc
     1561 atcagtaaaa aaacatatga gctgcgtaaa atgcatgttt ctcatatgct ttttttatta
     1621 ttatgtaaag aatgcgaaaa acgtttacat gatttttaca taacttaact atatttttac
     1681 agcaaccata cgagggattt acagtcgact gataaacttt agttgtaaat aagaaaggag
     1741 caataaaatg aaaaaagtat tgtttactct aggtatgatg ggaattcttt tagccggatg
     1801 cggctcagga tcaggaaata cagatggctc tgccacacaa aataattcaa gtgactcaaa
     1861 tgaacaagta aaaatcgttg cggtaggttc gacagcatta cagccgttag tcgatgcagc
     1921 acaagaaagc tttgtacaag aaaatccaaa ttatcaaatc tctgtacaag gaggaggaag
     1981 cggaactggt ttgagccaag tcgaagctgg agcagttact atcggaaact ctgatgtatt
     2041 tgcagaagaa agagacggag ttgatgcttc aaaattagtg gatcataaag tagcagtggt
     2101 cgggatggca ccaatcgtca ataaagatac agatgtcaaa gatatcacca aacaggaatt
     2161 gattgatatc tttacaggaa aaatcactaa ctggaaggaa gttggcggga aagatcaaaa
     2221 aatcaacgtc gtgaaccgtg caaacggcag cggtacccgc gcgacattcg aaaaatgggg
     2281 actggatggt gctacccctg tgcagtccca agaacaagat tcatcaggaa cagttcggca
     2341 gctggtaagt caaacaccag gagcaatcag ctatttagct ttctcatatc tcgatgattc
     2401 cacacaagca ctaagtatcg acggtgtaga accaaaagaa gagaatgtag cagataacag
     2461 ctggggaatc tggtcatatg aacatatgta cacgaacgga aaaccttccc ctgaagtcca
     2521 aaaattctta gactacatga tgacagaaga aatccaagaa ggacctgtca aagaattggg
     2581 ctatttacca atcacaatga tggaagtaga acgtgatcac gaaggaaata tcaaataact
     2641 agcagataaa catgccacgg tagtcgttac tattaaggaa aaatcaacta tgtttcccca
     2701 catatacatt tttcctttaa ttgtgaaaaa tagttcctca tattttgccg ttttttgagc
     2761 taataccgag ggtcagctga ttgaacaccg tttattcgaa aaaaagagct acgacataga
     2821 actgtcgtaa ctttttttgg ctcttcgtca aatcgtgttg gtgaagacaa atttgaataa
     2881 aacttagacc gatatgcggc gcgttctgtg cgccgcatat ttttttagtt ttcaaagatc
     2941 agaccttgtt gta
//
LOCUS       DAFFFQ010000160         2917 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000160
VERSION     DAFFFQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.167"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            204..1172
                     /locus_tag="LQB69_002941"
     CDS             204..1172
                     /locus_tag="LQB69_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL3527041.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="LQB69_002942"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="LQB69_002942"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL3527042.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="LQB69_002943"
     CDS             2202..2810
                     /locus_tag="LQB69_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL3527043.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaa
//
LOCUS       DAFFFQ010000161         2838 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000161
VERSION     DAFFFQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.169"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            192..599
                     /locus_tag="LQB69_002944"
     CDS             192..599
                     /locus_tag="LQB69_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527044.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDDICKKVCNGEENYWIGEEKLNEIY"
     gene            586..1038
                     /locus_tag="LQB69_002945"
     CDS             586..1038
                     /locus_tag="LQB69_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527045.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            1031..1429
                     /locus_tag="LQB69_002946"
     CDS             1031..1429
                     /locus_tag="LQB69_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527046.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            1741..1814
                     /locus_tag="LQB69_002947"
     tRNA            1741..1814
                     /locus_tag="LQB69_002947"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Ile,seq:cat)
     gene            2194..2355
                     /locus_tag="LQB69_002948"
                     /pseudo
     CDS             2194..2355
                     /locus_tag="LQB69_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            2505..2705
                     /locus_tag="LQB69_002949"
     CDS             2505..2705
                     /locus_tag="LQB69_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL3527047.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         1023 a          395 c          520 g          900 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctttc cttctattaa aatatcttga aatttactct gttaaaaaaa
      241 ataaattaat tggtgaaaat gattctccaa taaaaacacc tattgagcta agcttaagaa
      301 atcggatggg aatactccaa agatcggaaa tagtaaaaga acttttttat cataaattta
      361 gttatgaaat aagactagat gatacttatg agcattatag aattgttttt ttcgtataca
      421 attctataga agatgctact gcgactactg cattaacatt cggttttacg aaaaatggaa
      481 caatcaattc ggataaaacc cgtcaagctg ctacagaaag tgatgatata tgtaagaaag
      541 tatgcaatgg ggaagagaat tactggatag gggaggaaaa gttgaatgaa atctattaat
      601 gatttattaa aaaaacatcc tgatttatat aaaaaattaa attctgaaca aagaaacctg
      661 tccaaacaat tacttaaaat aagattaaaa aaacaaaaaa acgtgacaga attctcaaaa
      721 atgttaggaa taagtgaaaa aaaatatctt gaatttgagt tcggagatat gcatattcct
      781 gttgcagagt acaataaact tttgaaacaa gttgaaaatt tgtcctataa agattcaatg
      841 aatttcacta ttaaggttgc tggcggccct attaaatatg accgtattga aaataaacct
      901 tatattcgga ataaaagtta ctcagtattt aaaagtagaa agagaaaaga agtatcgtca
      961 aatatggtct atgctagtgt taattaccaa gatacgagcc gctatattgt agaaaaagag
     1021 gtatatgtac atggataaag gaaatagtgt tacttttgca cttgaaaata ttagtttgaa
     1081 taaaattcat tttgaattaa ctgataattt tagttatgga gatttagagt ttaatattga
     1141 acttggatat aaagaagacg aagcagagta tctagaaaaa aaacttgttt ctttacgagc
     1201 tcacattgta ggggggatgg aaggagcacc ggtagatatt ttactctttt cagttattcg
     1261 tgtttctaca aatgaacagc catataaaaa attagtagaa gaatctatgg gagagctagc
     1321 taaacctatc ctgaataaag cttctaattt gatttctaat ttatatagcg aagtaggagt
     1381 tatgcctaca gttattgatt tatatcagct atatagtcaa caaaaataaa atacaagtaa
     1441 tagagttatc tatttaaatt tgcaagacaa agacctcaac cagtcatcct tggttgaggc
     1501 ttttttgctc tgtaattaac tcgtatagat cctgcaagat agtttgtttc tgaataaatg
     1561 attgatatga agtatttaga tgactgtatt gacaactata aaaatcattc gataaaatag
     1621 tgatgttatc gcatatcttc actatcaccc ataatagtca cactccaagc tatgcgataa
     1681 caggtttgtt gccacacatt ctactggttg attgtttatg gctttatgtg gcaacaacca
     1741 gtacccttag cttagttggt cagagcagac ggctcataac cgtccggtcg taggttcgag
     1801 tcctacaggg tacataattt aatctatttg ataaaaaata aaattcaata atggtataat
     1861 aaaatatcgg ctcgtcgctt ccacagagat tttcgtggga ggaggtgtat gatttgtttg
     1921 aattcacttt ggagcacgtg ctcttgccat tgttaattgg tttaactttg gcattattta
     1981 gtcattggct agataaacat tagagccgac aatggcgctt actgtttcgc agcggtaagc
     2041 gtcttttttt gaaaaagaaa aaaacacaac gtattcgcag gtacgttgtg tttatggtgt
     2101 atatttgttt aaattcactt tcaattatta ttatacttgt taaataaatt gtgtcaatat
     2161 aaaagatatt tttatacgtg tactaaccta tattatcctt ctggtaatgt tattccatcg
     2221 cagcaggata tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact
     2281 gtactggacc acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg
     2341 atggaagaga agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg
     2401 taattttgtt tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat
     2461 gttacaattt aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag
     2521 taaaatggtt caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg
     2581 tattcgttca cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag
     2641 cagtgacttt cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag
     2701 cttaatttta tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc
     2761 gagtaacaaa aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat
     2821 gaaaaagtaa ataaattt
//
LOCUS       DAFFFQ010000162         2731 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000162
VERSION     DAFFFQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.170"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            179..775
                     /locus_tag="LQB69_002950"
     CDS             179..775
                     /locus_tag="LQB69_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL3527048.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="LQB69_002951"
     CDS             899..2698
                     /locus_tag="LQB69_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL3527049.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          776 a          468 c          516 g          971 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctataatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac a
//
LOCUS       DAFFFQ010000163         2636 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000163
VERSION     DAFFFQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.171"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            106..1305
                     /locus_tag="LQB69_002952"
     CDS             106..1305
                     /locus_tag="LQB69_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL3527050.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(1332..2600)
                     /locus_tag="LQB69_002953"
     CDS             complement(1332..2600)
                     /locus_tag="LQB69_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL3527051.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT          789 a          573 c          468 g          806 t
ORIGIN      
        1 aaaacatctt tccatcggaa agatgttttt ttgctttttc tagtttaaat aacagctttt
       61 ttcatctatt ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga
      121 gatctgatct ttttagttct ctatcttatt ggggtattct ctatcgttta ttggcgaaaa
      181 cagaagaagg atccttcctt gttcttttac ggcagtacgt ggacatttac ggtaatctta
      241 acttattttg tcgtgttgga aattttttat tcgacctttt attttttgat tccattgttg
      301 ttctttagta tctttgcttt ttcttatttc acagaaaaac gaaggctact gaatggtctt
      361 ttattcaatg ttttactcat cagtttcggc atctacttgt ttgtcttgct gtatgaaacg
      421 caaaatattt ttcttggcgg actgatcgcc ttgatcacga ttccattgct acttgtcctt
      481 ttatttggta tttatggatt gattgttttt cttttttgga acggggtcac agttttacgt
      541 agagaatcgc attcattagc taatctttta acgttgatac tagccatttt tcttacactg
      601 tttcttgttt ttgacttttt cttgttaaaa tatttgccgc aatggataaa tgctttgttt
      661 ttctgcgttc ccctgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc
      721 tcattcttgt atcagttcaa ccggcctcga tacaatcaag acttcatcgt tgtgttaggc
      781 gctggattga tcaatggaga aacagtttct cctcttctag ccaagcgaat caataaggca
      841 attgcttttt atcgggctca aagccgtgct acattaaatc cgcccatctt attgatgtct
      901 ggcgggcaag gagcagacga aaaagtacca gaggccattg cgatgaaaca atatgccatg
      961 gaacaaggaa tccctgaaag agacattctt gtcgaaacca attcgacaac cactttggaa
     1021 aacatgctct attcaaagga aatcatggat cagcaaatga aagggccata ccgcgcgatc
     1081 tttagttcca acaattacca catttttaga gccggtcttt atgcgcggca agccaaattg
     1141 aaagcaaatg gtattggtgc aaaaacagct ttctactact tgcctaatgc gtttttaaga
     1201 gaatacctcg ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt
     1261 ttcgcaggct ccgcattctt atcgctgatt actttacttt tctaaaaaaa caaggtgtga
     1321 caaaacatat gtcacacctt gtttttttct ttcattctct taactattgg caaaaagagt
     1381 atcccttttt tgaccgctac ttgctgagca gaataaatac cgctgttgcc tgaacacata
     1441 aagctgatca agcagatcat aaagaagtat actgccgctt gtgaaccgaa caattcgatc
     1501 cccatcacaa agcaggcaat cggtgtattt gtcgcaccag aaaatactcc gataaagccc
     1561 aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg ctagtgctgc accaagcgtt
     1621 gcaccaattt caaacaatgg tgtcacttct cccccttgat atcccgcgcc taaagacaaa
     1681 actgtgaaga ataacttccc aataaaatca tagacatgtg cctgtccatt aaatgattct
     1741 tgaagtaacg gaagactcaa tccgagataa cgttgtgttt gcaaaatcaa tgctgctgcc
     1801 acaactacag ctccacctat cgtatttcga caaacaggat gaggaatcca ttgtgcatat
     1861 cgcgctttta aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc
     1921 ccagcaacaa aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg
     1981 taatgtagat gagaaacacc atagctttct gtcatgaaat ttgcaaacag tgcagcaaag
     2041 aagctaggga agatcgcttc tgttcggact ttcccgattg ccaacacttc taatccaaat
     2101 aatgttccag ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata
     2161 atcaagattt cccgttctga tttatccaat cggaacaaac gtgccgtatt atctgctaac
     2221 gctcccccca tttggactgc tgttccttca cggccaacag atcccccaaa taaatgggta
     2281 gttatggttc caaacagtgt aagtggaata agccgcaacg ggattttttc ttctccacca
     2341 tttccttgat cgatcactaa gttgtttcca cgactagaaa gtccgccgtg atacgcatag
     2401 agataagcaa aaagcgcacc actaactggt agtagacaaa ggagccaagg atgtgatagt
     2461 cggatatctg tcacaactgt caggctattt aagaaaaaag cagacaagct ccccatgaat
     2521 ataccgagaa tcgctgtgat caccagccac ttcagcatat acactaaaat gcgtactgga
     2581 ctcggtaatt gtttttccat aaaaaattcc tcctcaataa aaaaacgcct actaaa
//
LOCUS       DAFFFQ010000164         2483 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000164
VERSION     DAFFFQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.172"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(1014..1304)
                     /locus_tag="LQB69_002954"
     CDS             complement(1014..1304)
                     /locus_tag="LQB69_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527052.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="LQB69_002955"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="LQB69_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DAFFFQ010000165         2478 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000165
VERSION     DAFFFQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.173"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..903
                     /locus_tag="LQB69_002956"
     CDS             <1..903
                     /locus_tag="LQB69_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL3527053.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..1824
                     /locus_tag="LQB69_002957"
     CDS             916..1824
                     /locus_tag="LQB69_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL3527054.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1824..2444
                     /locus_tag="LQB69_002958"
     CDS             1824..2444
                     /locus_tag="LQB69_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527055.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          852 a          367 c          566 g          693 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttcaga taatcacgaa gtcgaaaaag ttggaactgt agaattcccg
     1741 attgaagaag gagccttgat catcgcaacc gttgatgatc caaagcaaaa tgtgttcact
     1801 cttgagaaag tcggggtgat ctaatggcta aaactaaacg aatgatggaa acggatgaaa
     1861 aaacaggtgt acaacgccaa ttctttccaa tcacacatgc ttccgcagtt cttgggttag
     1921 aagaaataat agcaggagaa gcaacagttt tatctgttaa tggaaaaatt ggagccgtcg
     1981 tcattactaa agaagatttg ggattagaga atgttctcac agaattaccc tatgcaagtg
     2041 aagaagatga cggcattatc acggcagaaa tgtatcaaaa aattttaaac agtggagaag
     2101 gtgactacgt gttgccagtt gccactatcg accgtttggg tggcataaaa gttggtgaat
     2161 tattgacgat tgatgagaca gggaaagtct ctgcagtcag acaatctgat gtcaattttt
     2221 cgctggagtt aaaagaaaaa ctcgattcac tgaaaaatta tactgctgga gaaaacatca
     2281 ctattgatga agatggaaca attagttcta caggtggtag tgggactggt ggtgtcaatc
     2341 agtcatatgt agatcaaaaa ttccaagaag ctgtaaatca agcagaaaat tacacaaacg
     2401 aaagaattcc aaattttact tttgaaaaaa ttggggaggt atagagaatg acagatatcg
     2461 taaaaataaa acaaagca
//
LOCUS       DAFFFQ010000166         2468 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000166
VERSION     DAFFFQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.174"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(493..1785)
                     /locus_tag="LQB69_002959"
     CDS             complement(493..1785)
                     /locus_tag="LQB69_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3527056.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="LQB69_002960"
     CDS             complement(2059..2319)
                     /locus_tag="LQB69_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527057.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          823 a          459 c          372 g          814 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagt
//
LOCUS       DAFFFQ010000167         2426 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000167
VERSION     DAFFFQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.175"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..123)
                     /locus_tag="LQB69_002961"
     CDS             complement(<1..123)
                     /locus_tag="LQB69_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3527058.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGIN"
     gene            complement(197..478)
                     /locus_tag="LQB69_002962"
                     /pseudo
     CDS             complement(197..478)
                     /locus_tag="LQB69_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            940..2322
                     /locus_tag="LQB69_002963"
                     /pseudo
     CDS             940..2322
                     /locus_tag="LQB69_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          880 a          415 c          403 g          728 t
ORIGIN      
        1 atttattcca cgatcatata acaattcctg aacttcacga tagcttagat tgtaacgcag
       61 gtagtaacca acagcgacaa taatgacgtc ttttttgaat tgtttgcctt taaaatgatt
      121 cattactctg tcctctctgt cttttttctc aattttacac taaaatagat tttttggaaa
      181 actttgcaac agaaccgaaa tgagtatagt atgaattagt aatttggttt tcatctaagt
      241 ttagctttga tttactaaaa ttgatatctc gatacatgtt caatacatct aatacaaatc
      301 gagaatcttc tttcggaaat ggctcagaaa gatcactcca aatttcatca tcataatgat
      361 actcgtaacc ttctctcaat atttcgattt ttctttcata ataagatctg ttttggtcct
      421 caaatttagc caataactca aattgattta ctagtgacaa tctctgaaaa ctgtccatta
      481 aatgtttctt cctatcttgt tcattaacaa aaaattataa gcccttacaa cgaatattat
      541 atattaaatt taattgtagg tgaacacgaa ttttataaat atttatggtg ttaatgacaa
      601 atgatatccg actcctcttc atacgttctg gactatttgc taatagatta aaatttcagc
      661 atctatttct gaccataaat aaatgagctg ccgaaaagct gccaaatcag acttgaaaat
      721 aaaggatcaa aaacttttaa gtttctgacc ccgtaactca cacttcaaaa tcttacttaa
      781 aaaggagtct gaaagaaaaa aagagtattc aaaatttttt tctaactagc tacatcacat
      841 ctatgcattg acactcaaag aaccagaata agtcatcaga aagcaagggg gtgcggacat
      901 aatcctggac gaaaaaataa acttaggagg aatgtccgtg tgttttccca caaagagcgt
      961 gtaagggcaa ttcagttatt tttaaaatac gattgctctt atgctgcaac cattcgagaa
     1021 ttagggtatc cgtcgattgg tgcactacgg aaatggtata aagagtattt ggaatccgga
     1081 gagctccatc aggagcaccg aaaaaaatca aagtattctg aagaacaaaa acgaatagcg
     1141 gtgaatcatt attttgaata tggtcaatgt tatgcacgaa ccattcgcat gcttggttat
     1201 cctaatagag agtccttgcg gcagcggtgt gaagaacttg caccaggagc tagaaaactc
     1261 cgaaaaagtg cggtaaagtt aacacaagat caaaaagaag ctgtgttgaa aaaattctat
     1321 aaaccgcaaa ctaataggaa aaatttagcc gaatctggag gaattagtag agttactcta
     1381 tatcaatgga aaaacacggt tcttggaaag gattttccat tgtgtatgac tttaaaaaat
     1441 caggaaaaac aaaaagagct acttttaaag gaagtagaag acttacaaaa acaagtacac
     1501 caattacagc tcgaaaaagc attactcgaa ggagcggctg aactgctaaa aaaagaaaaa
     1561 ggcgtcaatc ttctttgtct atcaaatcaa gaaaaaacga tattgattga tgccttacga
     1621 aatcagttca ccttgaaaga gcttcttcaa cagctccagt ttcctaaaag cagctatttt
     1681 tatcaaaaac aagcacttga gaaaccagat aagtattaca aagaacgtca aattaattat
     1741 cacgattttc aatcataact tctgtgcgta tggttaccgt agaattcacc aagctttaaa
     1801 aaatatgggt aaaaagcttt ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct
     1861 atttgttaaa ttttctagac gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc
     1921 acaccctaat ttattaaatc ggaattttaa agcaaagaag ccaaatgaaa aatggttaac
     1981 agatattact gaatttagaa ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg
     2041 ttacgatggt gctattgtta gttggactat tggaacaaag ccaacttgct tcactctatg
     2101 tctaaaaagg gttattcacc agataattca gcgtgcgaag gtttctttgg acgattaaaa
     2161 aacgagatgt tttatcaacg tgactggaag aatacaacaa tcaatcagtt tataaacaaa
     2221 ttggatgatt atattcattg gtataataat caacgaatta agttatcttt aggagggcta
     2281 agccctcttc aatatcggaa gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata
     2341 tccgcacccc cttttttttg taaaaaacta ttgcacaata caagtagcct acggctatcg
     2401 aaatttccat aactttcgag caagaa
//
LOCUS       DAFFFQ010000168         2416 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000168
VERSION     DAFFFQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.176"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            2..1147
                     /locus_tag="LQB69_002964"
     CDS             2..1147
                     /locus_tag="LQB69_002964"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBL3527059.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1318..1776
                     /locus_tag="LQB69_002965"
     CDS             1318..1776
                     /locus_tag="LQB69_002965"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL3527060.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1935..2405
                     /locus_tag="LQB69_002966"
     CDS             1935..2405
                     /locus_tag="LQB69_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL3527061.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          807 a          454 c          455 g          700 t
ORIGIN      
        1 catgcacatt caaacaaaat tgatccacgg aggcatcagt gaagatccca caacaggagc
       61 tgtcagtgta cctatttatc aaacatccac ttatcggcaa gatggcgtcg gccagcctaa
      121 acaatatgaa tattcaagat ctggtaatcc tactcgtttt gcattggaag aactgattgc
      181 tgatttggaa ggcggtgttc gaggattcgc tttttcttct ggtctttctg ggattcatgc
      241 cgttttttct ctttttcaag ctggcgatca tatcttatta ggagatgatg tctatggagg
      301 aacattccgt ctatttgaca aagttcttac taaaaatggt ctcgagtaca cgatcataga
      361 tacgagtaat cttgataaaa tagaacaatc catcaaaccc aatacaaaag cactttatct
      421 tgaaacacct agcaatccgc ttctaaagat tactgattta gaaaaaagtg ccactctggc
      481 tcatcaacat ggattgatag ttattgctga caatacattt gctactcctt attttcaaag
      541 acctttagat ctcggttctg atatcgttgt ccatagcggt accaaatatc taggcggaca
      601 cagcgatgta gtagctggtt tagtgacttc taaccataaa gatctagccg atcagatcgg
      661 attttaccaa aatgctatcg gagccgtact tggtccccaa gatagctggt tacttcagcg
      721 cggaatcaag acactaagtg ttcgtatgga ggaacatcaa aaaaatgctt ttgtggtagc
      781 tgattttcta ttttctcatc cagcagtaga aaaggtctac tatccaggat tacccgatca
      841 tgaactccat ggagttgcta agcagcagat gagcggcttt agcggaatga tttcttttac
      901 tttaaaaaat gaagaatctg ctattccatt tgttgaatca ctccaactat ttactttagc
      961 agaaagttta ggtggcgtag aaagtttagt ggaaattcct tctgtcatga ctcatgcgtc
     1021 tattccaaaa gaaaaacgag aagaggctgg cataaaggac ggcttgatcc gtctttctgt
     1081 aggaattgag tatggtcagg atctcataaa cgatttagct caggcatttg acagaatcaa
     1141 gaactagaag tcccaataaa cgacttagca gaaaaatcta aaaaatattt cagattgcta
     1201 caccttggaa acgataagtg ttagaatcaa gtagaaagac gtttttgtaa atttcactaa
     1261 ataattcttt agtgaaaagc atcaaaacga atgaacaaac agataggagt cttacaaatg
     1321 agaatgaatg tcgaaagttt taatcttgac catacaaaag tcaaagcacc ttatgtacgt
     1381 gtagctgatc gaaaaacggg tgcgcatggc gatgtcatca ttaaatacga cgtccgcttt
     1441 aaacaaccaa ataaagaaca catggacatg ccaagtctac attcacttga gcacttgact
     1501 gccgaactaa tccgcaatca cgcagattac atcgtagact ggtctccaat gggatgccaa
     1561 acaggctttt acttgactgt attgaatcat gaaagctacg aagacatttt agatgttctt
     1621 gaagcaacaa tgaaagatgt tcttgaagct actgaagtac ctgcaagcaa tgaaacacaa
     1681 tgtggctggg ctgccagcca tacactagaa ggtgcacaag ctttggctcg tgaattttta
     1741 gcaaaacgcg atgaatggtc tgaagtagaa gcatagtttc taaaagaacg taaagatgag
     1801 aagcaatata gctatcttat cttcgtattt tattaaatat ttattgtctg aaaagagttc
     1861 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
     1921 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
     1981 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
     2041 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
     2101 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
     2161 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
     2221 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
     2281 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
     2341 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
     2401 tttaacttaa taaaaa
//
LOCUS       DAFFFQ010000169         2414 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000169
VERSION     DAFFFQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.177"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            155..2344
                     /locus_tag="LQB69_002967"
     CDS             155..2344
                     /locus_tag="LQB69_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3527062.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
BASE COUNT          917 a          325 c          397 g          775 t
ORIGIN      
        1 agccaactga ttgagaatta gaaacaatct gcagtaggga agaatcattt acacaaaatt
       61 attgacgctc ccttataatg agaactttta ctaacataat ttatgataaa cttactatat
      121 aatcatatta attaaatagt taggagatct gcttatgttt atatctactc ttcaaattcg
      181 aaattttaga aattatgaat ccgaaatctt tcgattcgat aatgagacaa atactattat
      241 aggtgagaat gattcaggga aaaccaatgc tcttactgca ttgagaatct tattagatga
      301 tagttattac tatagtagta aaacactaaa agaatcagat ttttttcatg gaattcaaaa
      361 tgggtggcaa gggcattgga ttattatctc tgcgacattt gaaggaatca gcgaagaaga
      421 atttgacaat gagatttgtg cttccatttc tttaaattct gaaagtcaaa caatcttaga
      481 agaattgatt tcaaatgcag ataaaggtgt aggtagtatt tctctattta ttagacctaa
      541 caaagctatt agaaagcaat tatttgatat ttccactgaa ttagactctc atcaatttaa
      601 cgaatttaga aattctattc gtctatccga ctatgaattt tactacacat ctaaatcaaa
      661 tctaaatttc tgtatcaatg aaaattatga taaattggta ggaagactca atgacaatat
      721 agctagtaat cctgaagaag acgatgaagc tctgttgggc tcaagaattg acatgtcaga
      781 tattttcaag catatttcgg ttgtttatgt tgacgctctt cgagatgttc ttagagaaat
      841 gaaaaacaat cgaaacccag tcaaaagaat catggaaaca attgaatcaa aaatatcatc
      901 agataatgtt gagtcattaa aaacaattat acagcaatta aatgagacta ttaccagtgt
      961 tcctgaaatt aggaaaattg gcgaaaatat taatcggcaa ctaaatagca taattggttc
     1021 agtgtattct cctaatcttc ttctttcctc aactatgtca gatgatatgg gttctttagc
     1081 aaaatttctt tcaatgaagc cagaacaaaa tatcgactta gacttactag ggttaggaca
     1141 tctaaatatg atttatttag ctttgaaaat agtcgaattt gaagcgtgtc gttcaagaga
     1201 actacttaat attatgttga ttgaagaacc agaagctcat attcatcatc atattcaaaa
     1261 aactctgttc gaaggattaa atcttcaaaa aaactatact cagattctca tgaccacaca
     1321 ttctgtacat ttagctgaag tgtctgaaat atctcggatg aatgtattaa aatcgtttaa
     1381 tggcaaatct atcgttatga atccagcaaa taatctgaac tcatttgcta aagaaaagtt
     1441 aaaaaagaac aatctaaatc taataaacgc agtagagaga tatttagatg ttaaaagaaa
     1501 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
     1561 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
     1621 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
     1681 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
     1741 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
     1801 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
     1861 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
     1921 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
     1981 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
     2041 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
     2101 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
     2161 cttgtacgag gaaactacag aattgtcagc tttatcagct agtttaaaaa caagtgatac
     2221 agctgatttg aatatagcaa taatttcttt attagagtac tttggtaaag atgaggatgt
     2281 tgtgtatcta atcaatcaga tagaaaatta cacaaaaata ctttatgctg aggtttcaaa
     2341 atgacaaata tcttcgaaaa aatcgtaaat gatttaaatt tagaacaaaa agatgttgtg
     2401 gaaagtgaag ataa
//
LOCUS       DAFFFQ010000170         2397 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000170
VERSION     DAFFFQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.178"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            206..2068
                     /locus_tag="LQB69_002968"
     CDS             206..2068
                     /locus_tag="LQB69_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3527063.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="LQB69_002969"
     CDS             2068..>2397
                     /locus_tag="LQB69_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL3527064.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          352 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtt atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAFFFQ010000171         2349 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000171
VERSION     DAFFFQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.179"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            253..1551
                     /locus_tag="LQB69_002970"
     CDS             253..1551
                     /locus_tag="LQB69_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL3527065.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="LQB69_002971"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="LQB69_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DAFFFQ010000172         2190 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000172
VERSION     DAFFFQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.180"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            353..1081
                     /locus_tag="LQB69_002972"
     CDS             353..1081
                     /locus_tag="LQB69_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL3527066.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1111..>2190
                     /locus_tag="LQB69_002973"
     CDS             1111..>2190
                     /locus_tag="LQB69_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBL3527067.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT          769 a          289 c          470 g          662 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc
      121 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
      181 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      241 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag
      301 taaataagta atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa
      361 gtttaaaaag aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc
      421 tcatactaaa caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga
      481 tgactctcaa atagagtatg tacccaatga gctaattgtc agtttaaagg ctacacaaag
      541 aatttcaaca gagaaaaata aaccgcaaaa tttattgggt gtaaaagtta tggtggaaaa
      601 agatttgact cttaggacaa atgaaagcga ttataataag cgagatcaag tattattagt
      661 gaaattgaac gaagaaatta gtgtggaaga ggcagtaaga acatttaatg cttctgatat
      721 ggtcaattat gcagagccaa actatgtggt tgaactagag ccgttgaatg atgttgagga
      781 gccaaataca actgattctt taggaaatcg atcaattatt cttaacgatc ctaattggtg
      841 taaacaagct aatttaagag agattagcat gccatccgct tgggaagaaa ccaagggttc
      901 taaggatgtg aaggtagcgg tcattgattc aggaattgat tatcaacatg aagatctttc
      961 tggaaatgta gatgaaagtt tgggatacga tttttttgac gatgatacag atcctatgga
     1021 tactatggga catggacaca tattgccggg attattggtg ctcaaacaaa caacgaatta
     1081 ggtgttgctg gaataaattg ggatgttacg atggtaccta tacgtgtatt tggtagtagt
     1141 ggaggtacca gtttggcaat cttaactgaa gctattaatt atgcatccca aaaagggttt
     1201 ccaattatca attttagtat aggtagtgtt gtttatagca gtacagaaaa agcaattaaa
     1261 aactatcctg gattattcgt agcagcagct ggaaataatg ggatagatat gactgataaa
     1321 cctttttacc cagcttcttt ggatatacct aatattatta ctgtaggaaa ttctagtggt
     1381 tatgaacgaa gttctgattc aaattatagg aaaacaggtg ttgatttatt cgctagaggg
     1441 tttcgtgttt ttagtacatt tctaggaagt aaatacacgt atatgtcagg tacctcgatg
     1501 gcaacacctc atgtcactgg tagtgctgca ttagcgttgt cactagatcc tgatctatct
     1561 actgatgaat taaaaaaaat cattttaaat agtgttgatt cattttatga actatctgat
     1621 ttatgtctta cagggggaag actaaatacg aataatgtcg tacgacaagt aacgggtaaa
     1681 cttactgcat ctaatatcac attaagatta aatagtgaat ggtcagatga aattgctaaa
     1741 gagatgtgtc acgttcactg gattgaccag tcaggaaatg atttgacgga tcaagtggtt
     1801 gttctaaaaa acgaagtgga tacatctaac tttggcgatt acgaagtagt ttttgcatca
     1861 cctgatttaa cagatcaaat ctcagttaca gtgaccgtac ctcgattacg taaacttacg
     1921 ctaggaaaag aaacagcaac cattgatatt ggttccgcat gggatagtgt catagccatt
     1981 gatacttttc aagtgaaagc tatagatgat tttgggaatg atgttacaaa tcaagtacag
     2041 gttactatta atgctgatat agatacaatt aattcaattc aagcagtgat taagtttgaa
     2101 gttccagaat taggcttagc ccaaattggt agactgaata ttactgcaaa aaatagtaca
     2161 atcaatggat tgaatgaagt tagcatcgag
//
LOCUS       DAFFFQ010000173         2027 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000173
VERSION     DAFFFQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.181"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            484..663
                     /locus_tag="LQB69_002974"
     CDS             484..663
                     /locus_tag="LQB69_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527068.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            722..1078
                     /locus_tag="LQB69_002975"
     CDS             722..1078
                     /locus_tag="LQB69_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527069.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          717 a          276 c          386 g          648 t
ORIGIN      
        1 tattacacca tgaaaaagga agacaacgaa aatgaataag gtgatttatc aactaacaac
       61 ggattccata tcaacgattt gaaagaaaaa aaggtcaata atcatttata ccgaaaagtg
      121 agaatgtgtg aaacaaggac aaaaaacgcc tagtaactaa ggaaatggtt cttatttcgc
      181 accaatcgct ccaaccgccc ccgccgctta ggtggtgcga aataaacggg caaagcccga
      241 taacttaaga gaaagaaggt gatccatttg aacaagatta ttcccttatt gttagtactg
      301 tttgttggcg aaatacttat gcgagatagt gcagctgtac agttaaacct cgctgtattt
      361 ttggtaatac tggtgttttg tttgatcttt tggcggtttg tattagtcct cgtgagtcta
      421 ctattcaaga tgattgcagc catatttttc atagggttat ttcgatgaaa ggagtgagta
      481 ccaatgccaa ttgcaatagg aaataaacgg ttacccgtga cattagatga aaagagacaa
      541 aaagaattgc agcaactaaa gcagaagtat ggcaaaagtg aatctaagat tatgtgtatt
      601 gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg atgtaccagc gctaaaaaag
      661 tgacgtcact ttttaatttg atctatataa gtgatatcac ttttaccaag gagggaatta
      721 aatgttaaaa actaaaaaga attcgtacaa attaaagctt agtatactat ctattatttg
      781 tcttttaggc atgataatta gtggtcattt gtatagagtg gcatatacgc agtttattga
      841 gtacaaggtt cactctttag gattagctgg ggatcgatac gttgaatatg tccattattc
      901 attaaaatgt tcttcatata ttcttctttg gcttattttt tcattgtttg aattgctaaa
      961 tttaatagca atatcttcat atcacttaga taagctaaat accaaatcta gccgttataa
     1021 cgaagtgttt aaggaaaatg tgaagttaga aagtgactta aacaagctaa aaaaataaaa
     1081 agaagaaaac ataagttaac taaaattatt ttattaagaa tgatggaata taaggataag
     1141 gttaccaaca taacgatcat tacttctctg atatgctatg gacttctatc aatcagtatt
     1201 ttgtctatag attatggagt aactatactg caagccttag ttttggctat gcctatgtat
     1261 ctatctttgt ttatcatgca tgaaatatat aagctatttg ttttatcaaa aaatgtagaa
     1321 atacgggtaa atattttggt gaatagttat caaaaagaac tcaaagaaag aagacggtgg
     1381 cggtatttat tgaaaggatg aaaaagtagg atttgacata ctggtattca gaaagtgccg
     1441 tcacttttaa aacaatggaa aaggtggaga taagatgaag aaaaaacaaa ctaaactatc
     1501 attagcagat atttaacaca gttaaccgca actgaagatg gtgttgttga atttgaacaa
     1561 tctgaaattg cggtagtaga tgatcgttac cttaaaatgc cttacttctt tgaccaagca
     1621 aaggtcattt gcctttgtca ctacgatggg gtacgtgatt ttaggcgtta aaaagtagtg
     1681 attatatcaa ctttacgagt aaatataaat ttagacatga aactgaatta tccaatggaa
     1741 aatcatggtt tataatctag aaaaattata gtaatgattg gattatatat attctttcgt
     1801 attttcgtac aactgtagct tgtcaaaatt gagaaattta accgattagg tactttacat
     1861 cgttatttgg gtaatttcta aaacatgaag cttaccaacg aataaactga gactatctta
     1921 ttaattgatc aatataagaa aatatctttg aggagatgat gtactatgtt ttcgtctatc
     1981 cacgattggt taggtggtct tttcggcggt ggaggtaaag gttttta
//
LOCUS       DAFFFQ010000174         2019 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000174
VERSION     DAFFFQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.182"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            101..571
                     /locus_tag="LQB69_002976"
     CDS             101..571
                     /locus_tag="LQB69_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL3527070.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="LQB69_002977"
     CDS             793..1896
                     /locus_tag="LQB69_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL3527071.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          327 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcata
//
LOCUS       DAFFFQ010000175         1972 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000175
VERSION     DAFFFQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.185"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LQB69_002978"
     CDS             complement(62..241)
                     /gene="rpmF"
                     /locus_tag="LQB69_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL3527072.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(299..868)
                     /locus_tag="LQB69_002979"
     CDS             complement(299..868)
                     /locus_tag="LQB69_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL3527073.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1054..1956
                     /locus_tag="LQB69_002980"
     CDS             1054..1956
                     /locus_tag="LQB69_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL3527074.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
BASE COUNT          587 a          419 c          332 g          634 t
ORIGIN      
        1 aaaataaacc tcaaggctcc atggttagcc ttgaggtttt acattttcta attaaaatgt
       61 attaagcttc ttttgacatt acgtccttac catcatagta accacaattt gcacatacgt
      121 gatggctttt tttcatttca ccacagtttg gacattcgtt taaccctttg attgtcaatt
      181 tgtagtgtgt acgacgtttt gcttttttag cttttgatgt tcttctagct ggtactgcca
      241 ttttgctaca cctccttgta aagtcttgta tctaaattag acacaatata ttccaatctt
      301 acttgttatc gtcgtctttt gatttagaat caaaaagttc agatagtttt gcaagacgag
      361 gatcaatttt attctcggct tcttccattt ttctgcgctg gtattcttct tcggaaacaa
      421 cttcccaatc attgcctttt ggaagttctg ttgctgcttc ctcttctttt gtcaataccc
      481 gcattggaat cgccaataag atgttgtctt caatcgactc tgctaaattc aataatggac
      541 cttcaattag caaaatctca tctgcagaca tttgttcatc tctactttgg aattgttctt
      601 ctgtcatgaa tacttcgtct acagaaaaat ccatgggcag ttcaacaggt tctagtgaac
      661 gtgttgaagg aactgtcaaa gtcgtctgca cagtgtaatg aacgatatag cctttcttat
      721 caactgagac caaaccttcg acttccactg gcgcaacatc aagtattaaa ttgtctcgct
      781 tcatgagagc ggctttcaag tcgagcgtct catgaaaagt taacggtgtc tcctggtatt
      841 ttctcaattc caataaagac catttcattt ttcatcactc ctaagcaaca aaagcaatta
      901 tacagggcaa aaagcatgtt gtcaatgaaa ttttcttgac accttgcagt tcataaaaaa
      961 acagaaaaat ctttattaga aagcctctgt ttgctatcat cctcagaatc atttacaata
     1021 atcattattc cattaaagga gcggatgatc ttcatgaaca cgaaagatat cgaatactat
     1081 gtccaaatcg tcaaacaaaa aaattttacg aaagtggcta attatttccg tgtgagccaa
     1141 cctactatta catatgcatt acaacgtctt gaaaaagagt ttgacaccgt acttattaca
     1201 agagatcgat cgcatcacga attaatcgtc acgccttctg gaaagcagct gtttctccat
     1261 gcacaaacca ttctccaaga gatttctact gcaagagctg aaatcactca gttgcaaaag
     1321 aaaaagcttc gttttggcat gcctcctatt atcggcaatg aatactttcc taaactgtca
     1381 agttacttta tcaagcatga attgatgaat caaattgaga ttaccgatgg aggctctcgg
     1441 gacttatacg ggatgatccg acaagggagg atcgatttag ctgtattagg ctcaacacag
     1501 ccaatcgtgg atcaatattt agaaacagag ttacttttgg acaaacgttt catgatcgtc
     1561 gtgccaccta cacatcctct ttccaagcag gatactgtct ctttcaaaga tatagcagat
     1621 gaacagtttg tactattgaa cgaacattat gtccaccccg cagcattcac taagctttct
     1681 aaacaaacac atactcatcc aaaagttgtt tatcaaagca atgacttgac gattttaaag
     1741 agtatggtcc gagaaaatgt gggtatcggt tttttggcag agattgctgt ccatcctgaa
     1801 gatcatttag tttgcctccc tctttcagat gaagttcagc ctcgcttctt gatttcattg
     1861 gcaaaacgaa cacagcagat cgtcacgcct cttcaagaac aagtagtcca agttatccat
     1921 gaattcgtta cagcacaaaa aaacagctct tcgtaaaaga agagctgttt tt
//
LOCUS       DAFFFQ010000176         1971 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000176
VERSION     DAFFFQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.186"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..535
                     /locus_tag="LQB69_002981"
     CDS             <1..535
                     /locus_tag="LQB69_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HBL3527075.1"
                     /translation="TFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVH
                     FLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLD
                     DGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMD
                     LHDEWIYSSRKYINFDK"
     gene            complement(665..>1971)
                     /locus_tag="LQB69_002982"
                     /note="aph(2'')-Ia"
     CDS             complement(665..>1971)
                     /locus_tag="LQB69_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL3527076.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYS
                     YISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECT
                     IDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNH
                     LLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKA
                     KEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          666 a          345 c          201 g          759 t
ORIGIN      
        1 aacatttttt gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga
       61 tgcgcacaaa ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag
      121 atgccaagtt cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc
      181 tttcagagaa gctgttaaag gaatttttaa gttcacagat attaacttag cgcgtgaggc
      241 taaaaatcga ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc
      301 attggatgat ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg
      361 actaaagagt accaatctaa ttgaacgact gaatcaagaa gtacgcagaa gagaaaagat
      421 tattcgcatc ttccccaatc aaacatcagc caatcgctta attggagccg ttcttatgga
      481 cctacatgat gaatggattt attcttcaag aaaatacatc aattttgata agtagaaatg
      541 gtaaaaacat tgtatagcat tttacacagg agtctggact tgactcactt cctttattat
      601 ttttcatttt tttaaactat ttaatactaa tgtcttttat aatagctttt catatattat
      661 ataatcaatc tttataagtc cttttataaa tttcttttct accattttcg ataaattcct
      721 gtttaatatt tttaattcca taaacaatag tttcaatagg ataatattct tcaactatat
      781 cttgatattc ttttgctttc tcaatatcta tatttccata cattcttaat atatcttctc
      841 caaaatttgt tcctatttct tcttcactat cttcaagtaa gtatataaaa tcacaatatt
      901 catctataat tccagaatct ccaaaatcaa ttattccagt taatctatta ttgccatcta
      961 acaatagatg attacaacta aaatcattat ggcataaaca ctttttaccc tcaaaaactg
     1021 ttgttgcatt tagtctttcc ataaaacttt ctatataatc tttttctata tcagttaaat
     1081 cattataaat agtttcacgc aacaatatat actcttctaa tacattttgt ttattatcaa
     1141 tagtacattc actaatatct gtataatcta aaccgtgcat ttgtcttaaa aaactggcaa
     1201 tatctcgttt taacaaattt tgttcttctt ctgacatagt agaataaatt tctggtgtta
     1261 aaaaagttcc tttaatttct ttataaccta gtatagataa ttcatcacta atatacgaat
     1321 attcaatatt aggaattttt acattagttt ctaaatttgt atttaaaaaa ttatatattg
     1381 ctttttcttt tgcataacct tttttcttat tagtactaaa ttttgtttta aaaatgtatt
     1441 cattattaac taaatatgcc acactatcat aaccactacc gattatttca atactatcta
     1501 ctttgaaatt atcaaagtaa tgctcaatta aatatttcat tgccttaaca tttgtggcat
     1561 tatcatcata tctatattcc attaaataac aatcttcttt tttgccctcg tgtaattcat
     1621 gttctggcaa atcttcaata attctaaaac cagatttttg gtatgccctt attgctcttg
     1681 gattattttt atgagggtct aaaataactg cattagcatt tctttctttt ttcaaaaatt
     1741 caaaaatcaa tttaatatat cttgtaccaa ttcctttact ccaataattt ggctctccta
     1801 taaattgatc cataccatag actatctcat cagtttttgg ataatgataa tcagtatata
     1861 actcatcata cattttatat atttgtccat atccaatagg aacattgtta tattcaataa
     1921 ttactctaaa aacttcatct tcccaaggct ctgtataatg tttttttaat g
//
LOCUS       DAFFFQ010000177         1943 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000177
VERSION     DAFFFQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.187"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(717..>1943)
                     /locus_tag="LQB69_002983"
     CDS             complement(717..>1943)
                     /locus_tag="LQB69_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL3527077.1"
                     /translation="SRDSSVQQEEMQKIIEEYNQYYNTSWSLADIERYNGDINNRLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEEQIG
                     VYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDARE
                     EIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSN
                     EWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMKRAW
                     RKMLDDELIPLENELR"
BASE COUNT          575 a          387 c          289 g          692 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtattata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gct
//
LOCUS       DAFFFQ010000178         1752 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000178
VERSION     DAFFFQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.188"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(192..569)
                     /locus_tag="LQB69_002984"
     CDS             complement(192..569)
                     /locus_tag="LQB69_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527078.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(869..1750)
                     /gene="galU"
                     /locus_tag="LQB69_002985"
     CDS             complement(869..1750)
                     /gene="galU"
                     /locus_tag="LQB69_002985"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL3527079.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          503 a          381 c          254 g          614 t
ORIGIN      
        1 aaacattggt atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga
       61 tacaacaagc attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac
      121 caattaaata aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata
      181 cctactaaat attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta
      241 gaaaaagtag ttgtatgtct accttttctg cctatccgag actttctttt catatatggg
      301 atataaatga ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta
      361 cctaacgcca attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa
      421 atcagaaacg tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat
      481 agtggcaaca caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc
      541 gactgtgcaa cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa
      601 aattcgcctt tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat
      661 cattttcttt tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct
      721 attcacgaaa gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa
      781 aactaaaacg acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt
      841 tcattctcag caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact
      901 ggataatata tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat
      961 ttgtcttcat atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg
     1021 catagaccgc ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc
     1081 ctgctcctgg ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc
     1141 cgataatggc taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta
     1201 ctctgcaacg acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt
     1261 cattttctgg tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca
     1321 tcaattgttg agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa
     1381 acggttcatt tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat
     1441 gagattgacg gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt
     1501 ctgttttttc cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt
     1561 caatgggtct tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg
     1621 cttcttccac aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca
     1681 tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt
     1741 tgaccttcac tt
//
LOCUS       DAFFFQ010000179         1718 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000179
VERSION     DAFFFQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.189"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(606..962)
                     /locus_tag="LQB69_002986"
     CDS             complement(606..962)
                     /locus_tag="LQB69_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL3527080.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="LQB69_002987"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="LQB69_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL3527081.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          529 a          315 c          242 g          632 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aaggactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcac
//
LOCUS       DAFFFQ010000180         1709 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000180
VERSION     DAFFFQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.190"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            8..>1709
                     /locus_tag="LQB69_002988"
     CDS             8..>1709
                     /locus_tag="LQB69_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL3527082.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     A"
BASE COUNT          591 a          302 c          369 g          447 t
ORIGIN      
        1 aagataaatg aaaattagtc accgtgagga agcggaagtc gaagaacagt tgatccgtgt
       61 attgggggaa gggcataatc agtggacgta tcgtcccgat ctaaaatcag aagaagatct
      121 ttgggtcaat ttacgccaaa aaatcatctc aaataatcaa gcagaactga atgattttcc
      181 tttgaccgat aaagaatttg aaacgatcaa aacagaattg ttactacgaa cgaaaacccc
      241 ctttgatgct gcgaaatggt taaaaggtga aaatggaatg gctcggatca cgattgaacg
      301 ggaagatccg caactgggtt ctgtctcgct cattctttat tctaatcaag acattggtgg
      361 aggaatctct acctatgaag tggtccatca aattgcaaaa cgtggaagta acatagaagc
      421 acgggatcga cgttttgacg tcaccttact gatcaatgga ttaccgattg tccaaatcga
      481 gctgaaacaa gtaaccgcaa aagatagttt ttatcaggcg tttaaccaaa tcaaaaaata
      541 tgcagaagaa ggtatgtttc ggaacaatat cttttcgacc ttacagttgt ttgtggtttc
      601 caatgaacaa accactcggt attttgcaaa tgccttacca aaggatttgc atcctaagtt
      661 tttatttagt tggcgaacga aagataataa aaaggtagaa aacttatatg agttttgcaa
      721 acaagtgcta aatatcccgg atgctcatcg cttgattgcg gattatacga ttgtcagtga
      781 agatcaagac aataaaatcc tcatggtgtt acatccttat caagtacatg cgattcaagc
      841 cttgtttatt gctgcaaata aacatcaatc aggatatgtg tggcatgcga ctggttcagg
      901 aaaaacgttg actagttttg tatctactaa gcttttagca cgaaaatcgg ggatcgatcg
      961 gacgatcatg ttagtggacc gcaaagattt agataatcaa accaccacag agtttacgaa
     1021 atttgcttcg gaatttaata ctgggatttc ttccgggaac gccaaagcga atagcttgat
     1081 cgtgggaaca ggaagtgcca aagagttaag tgaaactctg ttggcagatg ccaatgcaaa
     1141 tgttgtgatt attacaaccc gtcaaaagct agatgcggcg ttgaaatatg caaaaaaaca
     1201 agaagagaaa aaaggaacca atcgcttcca aaaattaatg gggcaacata tcgtgtttgt
     1261 agtagatgag tgtcatcgag cgttaagtgc tgaaaatatg gaagaaatca aaaaaatgtt
     1321 tccgaaatcc acttggtttg gctttacagg tacaccgatt ttccctgaga atcggaaaca
     1381 agccaaaggg caattggcac gtacgaccca tgatcagtat ggagaggtct tgcataccta
     1441 taccatcaag aatgcattag aagatggctc ggttttagga tttcaagtag agcatgaaaa
     1501 caccgtggag ccgacttcat tagagaataa aatctatcgg aaattaaaag aagtagaaac
     1561 atatgcagag tattcaccgg aacagatcaa tcgtatgatt gatcaaatgg agcctgtcaa
     1621 aaaagaaagt tatttggacc ctactgtttt tgaagcagat gaacatatcc aaaaagtcat
     1681 tcataaaatg ttccgtccag acaatgcct
//
LOCUS       DAFFFQ010000181         1685 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000181
VERSION     DAFFFQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.191"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(45..779)
                     /locus_tag="LQB69_002989"
     CDS             complement(45..779)
                     /locus_tag="LQB69_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL3527083.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="LQB69_002990"
     CDS             1106..1516
                     /locus_tag="LQB69_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL3527084.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..>1685
                     /locus_tag="LQB69_002991"
     CDS             1509..>1685
                     /locus_tag="LQB69_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL3527085.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYN"
BASE COUNT          536 a          294 c          325 g          530 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataat
//
LOCUS       DAFFFQ010000182         1655 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000182
VERSION     DAFFFQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.192"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            200..742
                     /locus_tag="LQB69_002992"
     CDS             200..742
                     /locus_tag="LQB69_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527086.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="LQB69_002993"
     CDS             898..1347
                     /locus_tag="LQB69_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL3527087.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1377..>1655)
                     /locus_tag="LQB69_002994"
     CDS             complement(1377..>1655)
                     /locus_tag="LQB69_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527088.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          624 a          236 c          319 g          476 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tccgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attcc
//
LOCUS       DAFFFQ010000183         1639 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000183
VERSION     DAFFFQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.194"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(186..1505)
                     /locus_tag="LQB69_002995"
     CDS             complement(186..1505)
                     /locus_tag="LQB69_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL3527089.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          486 a          301 c          325 g          527 t
ORIGIN      
        1 ttaattcttt ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt
       61 gactaaaaag ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg
      121 taaggcattt tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg
      181 ttggattaga tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc
      241 tgatacacat gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc
      301 ccagcaactt tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct
      361 tcaaagccaa tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt
      421 cggacatgat tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc
      481 gcttctttaa tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca
      541 ggagaaacat tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc
      601 gcttcacgtg ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa
      661 gtggtcgaga agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact
      721 gatttctcag ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc
      781 ttttttgatt cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc
      841 aagatgtcag tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc
      901 tttacgcctt ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca
      961 gtcaatccgt caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa
     1021 ttcgtttgtt cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat
     1081 tccgtatcat tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt
     1141 tcatggatat tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg
     1201 agttctttcc tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca
     1261 gaatcagctg agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg
     1321 tcatgagtaa aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa
     1381 aaaccaaaga tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga
     1441 ccacctgtat gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta
     1501 gtcatgaaaa gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg
     1561 gttctttttt catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc
     1621 agtttcacat ggttttaat
//
LOCUS       DAFFFQ010000184         1583 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000184
VERSION     DAFFFQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.195"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            292..1479
                     /locus_tag="LQB69_002996"
     CDS             292..1479
                     /locus_tag="LQB69_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3527090.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          315 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta ctt
//
LOCUS       DAFFFQ010000185         1579 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000185
VERSION     DAFFFQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.196"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            129..1298
                     /locus_tag="LQB69_002997"
     CDS             129..1298
                     /locus_tag="LQB69_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3527091.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          567 a          254 c          258 g          500 t
ORIGIN      
        1 aatatcccat attttaaaat aaaagatagc gtaaaatatt ttgtgtaaat agaaatttag
       61 ggtagaaaaa aaagtccatt ctgtaaaatt aaagttacca cacagaaatt ttataggagg
      121 actttctcat gactaaattt actatagaaa ttatgcaaac actactcaat aaaggggatt
      181 tagatgaatt atttcaggat catttagagc gagcgattaa ttcacttcta caagcagagc
      241 taactgcttt tttagattac gaaaaatatg atcgaaatgg attcaattca ggaaattccc
      301 gtaacgggaa ttattcacgt acatttaaaa cagaatatgg cgagttgaat ttaactattc
      361 ctagagatcg gaatggcgaa ttttctcaac aaacactacc agcatataag cgaaccaatg
      421 attcattaga aacgaccatt atccaattat ttcaaaaagg cattactatg gctgaaatct
      481 ccaaattaat tgaaaaaatg tatggtcacc actatacgcc acaaacaatc tccaacatga
      541 gtaaattggt ggctgaagat gttttagctt ttaaagaaag aacgttagaa gccaattatt
      601 ccgttatatt tatggatgcg acgcatatcc ctgtgaagcg acagactgtt tcgaaggaag
      661 cagtgtatat tacgattggt attcgtttgg atggaaccaa agaagttctt gggtttacta
      721 ttgccccaac tgagtctgct tatatttgga aagaagttct tcaagatctt agaaaacgtg
      781 ggttagaaga agttttatta gtagtgacag acggattaag ctgtattgaa gaaagtatcc
      841 atagtgtgta tccgaatgcc caatttcaac aatgttgtgt gcatgtatct agaaatatcg
      901 ctcataaagt tcgtgttcga ggtcgaaaag aaatttgtga ggatttcaaa ttggtttacc
      961 aagcgaattc aaaagaagag gcattggatc acatcgactt tatgactagg aaatggaaaa
     1021 agcagtatcc aagagtcgtc aatttactct tgaatcctgc cctattaacc ttttataatt
     1081 tccctcacgc catcagacga acaatttatt cgacgaacct gattgaaggc tttaataagc
     1141 agctaaaacg atatactcga agaaaagaac aattccctaa tgaagaatct ctagagagat
     1201 tcttcatttc tcaatttaat caatataacc aaaaattttt aggtagaatt cacaagggat
     1261 ttaaagaaat tcaggataca ttagagtcga tgatttaact gtaattaaca gaatggattt
     1321 tccatttaca tataattctt gacgctacca taatctaaat aaccatcata ttcatctact
     1381 acttatataa ttgagtttta ttaaactttt taagtttcta ttctatcaaa tatttaaaca
     1441 atctctgcat ccgaaaagac tacctcgtat agttttacta acaatatttt tattagcgtg
     1501 ttcaaatcct gatttcttgt atcaattatt ttctgttcac tttcaataag aaaaaagtct
     1561 ttacttccat ggataataa
//
LOCUS       DAFFFQ010000186         1559 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000186
VERSION     DAFFFQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.197"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            32..739
                     /locus_tag="LQB69_002998"
     CDS             32..739
                     /locus_tag="LQB69_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527092.1"
                     /translation="MNNVVYQLTMEDISRFLTNKEHLNEVIRRIQKDFSCMEEMKYFV
                     QNVEEQLYLESETSQTKVTVSNNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKS
                     ELVENFISYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGIDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKQMPYQRFERKKGQ"
     gene            1120..1299
                     /locus_tag="LQB69_002999"
     CDS             1120..1299
                     /locus_tag="LQB69_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527093.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFNIPALKK"
BASE COUNT          605 a          231 c          305 g          418 t
ORIGIN      
        1 tattacacca tgaaaaagga ggacaacgaa aatgaataat gtagtttatc aactaacaat
       61 ggaagacatt tctcgttttt taacaaacaa agaacatttg aatgaagtaa tcagacggat
      121 tcaaaaagac tttagttgta tggaagaaat gaagtacttt gttcaaaacg ttgaagaaca
      181 gttatatttg gaaagcgaaa cgagtcaaac aaaggtcaca gtcagcaata atcgtttaga
      241 tgtgtataaa cgtcgtcctg aatcattaaa aaaaggagcg gagcttctac gactagaaac
      301 agaaaaatac gtcatgaatg tttacctaga aaaaagtgag ttggtcgaaa attttattag
      361 ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
      421 catggaatta atggaagaag aaatggaaga gttaaacagt aaagatgcga cggtagacga
      481 ttattacctt gtatttacta gatttttaac gtgtggaacg gaatctatcg tgctaatggg
      541 aatagattac gaactcgctg tacccgaagc gtttttgagg gaatatgtag aagaagtaga
      601 taacccaggg gatgattggt acacgacttt aactgatttt ttagacacct acacttacga
      661 cgatacagaa gaaatccaaa atgagcttga ccgtaaacaa atgccatatc aacgatttga
      721 aaggaaaaaa ggtcaataat catttatacc gaaaagtgag aatgtgtgaa ataagaacaa
      781 aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa ccgcccccgc
      841 cgcttaggtg gtgcgaaata agcgagcaaa gcccgctaac caaagagaaa gaaggagatt
      901 tatatgaaaa agattattcc cttattatta gtactatttg ttggcgaaat gcttatgcgt
      961 catgctgcaa ctgtgcattt aaacttcgta gtatttttgg taatactggt gttttgtttg
     1021 accttttggc ggtttgtatt agccctcgtg agtttactat tcaagatgat tgcagccata
     1081 tttttcatag gattatttcg atgagaggag agagtatcaa tgccaattgc aataggaaat
     1141 aaacggttac ccgttacgtt ggatgaaaag agacaaaaag aattacagca acttaaacag
     1201 aaatacggta aaagtgaatc caggattatg tgcattgcgt tagatttatt gattgcgcag
     1261 gaaaaagcag gattcaacat accagcgctc aaaaagtgac gtcacttttt gacttaaaaa
     1321 ataaaagtga tagcactttt atatagtaaa aagaagaata aacgttaagg gatagaaaaa
     1381 gagaaaagaa caaaaaaaga gctaacagta ccattgttag ctcttatgca gtaatgaaaa
     1441 ccaagccttt aaaaaaggag atgtttcatc acaaacacaa acagaaaaat aaaaaaatca
     1501 aaaatctaat aatcataaat tgctaccgaa gcacctttta ttataattaa tcaaatata
//
LOCUS       DAFFFQ010000187         1502 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000187
VERSION     DAFFFQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.198"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(312..611)
                     /locus_tag="LQB69_003000"
     CDS             complement(312..611)
                     /locus_tag="LQB69_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527094.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(617..847)
                     /locus_tag="LQB69_003001"
     CDS             complement(617..847)
                     /locus_tag="LQB69_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527095.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1097..1297)
                     /locus_tag="LQB69_003002"
     CDS             complement(1097..1297)
                     /locus_tag="LQB69_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL3527096.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          533 a          243 c          219 g          507 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga
      121 aaaaaagtga gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga
      181 ttttatagca ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa
      241 cttaaaaaat atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta
      301 ggtagcctac tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca
      361 taacgccgat gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat
      421 acgtctttct tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag
      481 tagagattaa ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc
      541 ttataaaaat ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca
      601 ttaaaaagca taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat
      661 atcgagtttt tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt
      721 tattttcgga aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt
      781 cgttgctttg ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt
      841 ccatcataaa gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga
      901 atttattata ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa
      961 atcgaattta ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata
     1021 acaaacacaa cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc
     1081 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
     1141 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
     1201 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
     1261 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
     1321 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
     1381 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
     1441 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
     1501 aa
//
LOCUS       DAFFFQ010000188         1456 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000188
VERSION     DAFFFQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.199"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            108..1040
                     /locus_tag="LQB69_003003"
     CDS             108..1040
                     /locus_tag="LQB69_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL3527097.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="LQB69_003004"
     CDS             1112..>1456
                     /locus_tag="LQB69_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527098.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAFFFQ010000189         1425 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.200, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000189
VERSION     DAFFFQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.200"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            4..>1425
                     /locus_tag="LQB69_003005"
     CDS             4..>1425
                     /locus_tag="LQB69_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527099.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT          593 a          224 c          318 g          290 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaa
//
LOCUS       DAFFFQ010000190         1422 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000190
VERSION     DAFFFQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.201"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            1..>1422
                     /locus_tag="LQB69_003006"
     CDS             1..>1422
                     /locus_tag="LQB69_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527100.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     VPTDFLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VREIEATPEDKKRIESGQIEVIKK"
BASE COUNT          591 a          222 c          317 g          292 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaatta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccagagaaag
      121 accaacggtc aaccaatgaa cgataagaga aatgggcatt cttggtttag gcaacaagag
      181 cgaatcgaaa ataaagtgca tagtctgaga gaagagatca agcaacaaga aaaacaagta
      241 gaaaaacttg aaagacaaga agagataaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgagaatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcgactt tttcagccgc tacgatccga aaataccaac ggaaattgga aacattagaa
      421 cagctgaaag aaagatcgga aaaaggaaaa gaaaaccttc ttcctgaagt acaagcaatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccga ccatttattt tctgaaaggc
      541 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
      661 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aa
//
LOCUS       DAFFFQ010000191         1416 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000191
VERSION     DAFFFQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.202"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..106
                     /locus_tag="LQB69_003007"
     CDS             <1..106
                     /locus_tag="LQB69_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3527101.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="LQB69_003008"
     CDS             complement(359..508)
                     /locus_tag="LQB69_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527102.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="LQB69_003009"
     CDS             970..1182
                     /locus_tag="LQB69_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527103.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          523 a          212 c          236 g          445 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatg
//
LOCUS       DAFFFQ010000192         1412 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000192
VERSION     DAFFFQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.203"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            98..1390
                     /locus_tag="LQB69_003010"
     CDS             98..1390
                     /locus_tag="LQB69_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3527104.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          461 a          288 c          256 g          407 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg
     1321 ctttcgtagc ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat
     1381 tttcaactaa aaaagatcaa gaggaactga tc
//
LOCUS       DAFFFQ010000193         1394 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.204, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000193
VERSION     DAFFFQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.204"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            48..1349
                     /locus_tag="LQB69_003011"
     CDS             48..1349
                     /locus_tag="LQB69_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3527105.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          451 a          283 c          255 g          405 t
ORIGIN      
        1 ctccgttaga attgaatcac cacaacacaa tcaaaggagc gttctgtatg cctgatcttc
       61 atcatatcaa actactactt ggaatcaaag ataaaaacat tttcatcaca aatgtggaat
      121 cgaagagcat taagaatatc aagagtcttg ttgtttctgc gacgttaagt aaagaaattc
      181 gtcgctgtcc tttgtgtaaa cagatgaatc atgaaggcat gatcgttaag aacggaaaga
      241 aaaaatcgct cattcaactt aataaatgtg cgaaccaact gacttatctt gctttagcga
      301 aacaacgcta ccactgtcgt ggctgccaca cctattttac ggcgaatacc tacattgtcg
      361 atagaaactg ctttatcgct aaacaagtgc gctataaaat tttagaagag ctaactgaaa
      421 aacaagcaat gacgacaatt gctaagcatt gcggcgtttc ttggtcgacc gtttctcgga
      481 cactcgcttc tctgatcccc atgacaaaag taaaaagaaa ttggttaccg cgttgtttat
      541 taatggatga atttcgttca ctgaaaaatc aagtcggtcc ctactccttt tcttgtatgg
      601 atggcgatac tggaaaatta ctggatattc ttccttcacg gaagaaaaag gctctcgttt
      661 cttattttat gcaatttgaa cgacgcgcac gattgaaggt aaaggtcatc gtgacggaca
      721 tgaatgtttc ttatgcctct ctgataaagg aatgttttcc aaaggcaaaa ctcgttgtgg
      781 atcgttttca cattgtcaaa catctcattc gtaaatttga ggatattcgc gtacgaatca
      841 tgaaaagctt tgatcgaaac gatccaacac aagccaaata ttatcgacaa ttaaaggcgt
      901 tgtctcggct tctgatcaaa cgacaggaca agcttgttta tgacaaatgg accaaatggc
      961 gcaattttgg ttgggcgtat ctcaccgaaa gtgaagtggt ggatcgtctg ctttcgatct
     1021 ctgacgaatt acgaatcgcc tatttttact atcaagaaat tcttcaagcg tttcacgaca
     1081 aagaggcgga cactttcttt aaacttgtaa gaacgatgcc caattccgtt ccaaaagaac
     1141 tgcattctat aaaaaaagct tttataaact atgaatccgg cattcgttta gccctcgaat
     1201 tgccttattc aaatgccaaa atagaaaatc tgcataccca catcaaagca ttgaaacgag
     1261 tcgcttatgg ctttcgaagc tttcggaaaa tgaagaccag aattttctta ctaaataatc
     1321 tgattaccta tgaatcaaaa aacatctaaa gtagaaaagg aattctacct tagatgttct
     1381 tatctcactc tatc
//
LOCUS       DAFFFQ010000194         1374 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000194
VERSION     DAFFFQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.205"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            33..1372
                     /locus_tag="LQB69_003012"
     CDS             join(33..474,474..1372)
                     /locus_tag="LQB69_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL3527106.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          576 a          177 c          239 g          382 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DAFFFQ010000195         1362 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000195
VERSION     DAFFFQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.206"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            82..>1362
                     /locus_tag="LQB69_003013"
     CDS             82..>1362
                     /locus_tag="LQB69_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL3527107.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          462 a          255 c          259 g          386 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFFFQ010000196         1340 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000196
VERSION     DAFFFQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.207"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            15..1310
                     /locus_tag="LQB69_003014"
     CDS             15..1310
                     /locus_tag="LQB69_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL3527108.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          418 a          265 c          259 g          398 t
ORIGIN      
        1 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
       61 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      121 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      181 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      241 ctgagtatcg cacttatctg gagctgaaga aacaacgatt taaatgtaag tcttgccaaa
      301 gaacattcgt agcggatacc tctgttgctg aaaaacactg tttcattagt caaaaagttc
      361 gttggtctgt cattgctcgc ttaaaagaga atacttccat gacggaaatc gctcgtcaga
      421 aaaatatttc cacttcttcg gtctatcgtg tcatgaagcg attctaccgc ccattgaatc
      481 ctttcaaaca gactttgcca aaggtcttat gctttgatga attcaaatcg gttcgaagtg
      541 ttgccagtgc aatgagtttt atcatgatgg acggacaaag ccatgcatta ttggatattg
      601 tcgaaaatcg acgcttgcct tatttagaac gctatttttc tcggttttca ttagcgactc
      661 gtgaagccgt tcaattgatt gttattgata tgtatgcgcc ttatgtttcg ttggtgaaaa
      721 agcttttccc gaatgctcag ttgatcatcg atcgtttcca catcgtccaa catattggca
      781 gaactttctt gaatcatcga gtgaaagaaa ccacggtacg acttaaagga aattcccact
      841 ctcaacgagg gttagggaaa aaattgaagc gctattggaa actcctacaa aaagaagaag
      901 caaagttaaa ctatgaaaaa cgggtttggc gtgccagttt taaagattat ttgactgaaa
      961 gtgagattgc cgatcgactg ttagctgctt gtcccaactt acgccaaggc tatcagttat
     1021 accaagacat tctttatgcg gtaaagaaaa gagatcagtc actatttgag gattgtttaa
     1081 ctagagacat tagcggtctg ccagaaacct atacaaccac tttacgaacg tttaaaaagt
     1141 ttctgccaca gatccaaaat gcgcttcact acagctattc aaacggacca ttggaatgct
     1201 taaacaacca catcaaagta ttaaaacgca atgcgtacgg ctttcgcaac ttttacaatt
     1261 tcaaactacg aattatgatt cgtcatggca aaacattttt aaccaaataa acagaacaga
     1321 aagaggtttt cttctttctg
//
LOCUS       DAFFFQ010000197         1328 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.208, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000197
VERSION     DAFFFQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.208"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            82..>1328
                     /locus_tag="LQB69_003015"
     CDS             82..>1328
                     /locus_tag="LQB69_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL3527109.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          451 a          258 c          253 g          366 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaa
//
LOCUS       DAFFFQ010000198         1297 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000198
VERSION     DAFFFQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.209"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            141..398
                     /locus_tag="LQB69_003016"
     CDS             141..398
                     /locus_tag="LQB69_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527110.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1061)
                     /locus_tag="LQB69_003017"
     CDS             complement(630..1061)
                     /locus_tag="LQB69_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBL3527111.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaa
//
LOCUS       DAFFFQ010000199         1294 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000199
VERSION     DAFFFQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.210"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1244
                     /locus_tag="LQB69_003018"
     CDS             <1..1244
                     /locus_tag="LQB69_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL3527112.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFFFQ010000200         1292 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000200
VERSION     DAFFFQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.211"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            3..968
                     /locus_tag="LQB69_003019"
     CDS             3..968
                     /locus_tag="LQB69_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL3527113.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          421 a          201 c          300 g          370 t
ORIGIN      
        1 atttgagtaa acgtggcacg atcataaatg agtgggaaaa tttaacagcc ctatctaaca
       61 ggcggttaag tgtgcaaact ttgaccaccc cccttaaaat acagtggtca attgaccgaa
      121 ttaccattgt tggaaaactc aaagagaata tttattatca cacgcaaaat gatgtattga
      181 ttttagactt tgaacagtta atgcgactga atgaaaataa tggttatgtt aaatctgttg
      241 gtctaaatgg ttggcaaatt gtggataagc atgatgagaa tattgcttac attgaaattt
      301 tgaaatatca agaagggcaa gggcgaattg actttaaccc aaataaaatt agccaatttt
      361 tggcgggttc gatgaagaac tttatccatg atttattttt agaaccacat ttctcacgtg
      421 ctgatattgc ttgcgacatt atcgatgtgc ctgatgattt tattacgcaa tatcgtgtgg
      481 ttgaccctgt ttcattcaaa ccaatctatg ggcggtctgg gaagctggaa acggcatatt
      541 ggggtagtcg tgccagtgag cgacaaataa ggatgtacaa caaaaaattg gaacaagaaa
      601 ccaaacggaa aattgtacca ccggaaataa aaacttggtg gcgactggaa ttgcagttac
      661 ggcgtggaaa agctactgat tggtatgcga tggttcatga aagtttagac agtttcgcaa
      721 gtcctcattt tttgccgact gatacaaagc atactgatag aattatgatt gcaggattga
      781 tttcagatca aaatttatgg gctggcttgg ctcgtcatac aaaatataaa tatcgaaatt
      841 tgttgaaaaa agaaagccaa agtgatgagt tagttaatca tttacgagag acattttcgg
      901 aatcggcaga tgttttgaaa aaagaattgg atacgtggct tttggggttg gacgtgacgg
      961 aagaatgagt ggatgaattt ttgtccactt ttttattttc agcacaagtc ggcatggtgg
     1021 gtcgtgcgtt tgcagcaaga gcgaaaaaac gctaacgaat ccgcaaatgc cgacttggat
     1081 aaaacagttg cgcaaagaga cttggctttt tataataaat ttagggatat cccagtgtca
     1141 ctctttgggg taattcgggg gaaggagtta cccctcactc tggaaaatta gagcgaagca
     1201 gacgagatta tcccaatgaa tatgacggct tactcggagt gataccgagt aaaaaagtca
     1261 gataagtgag agtaaattta cgtcaatgtt ta
//
LOCUS       DAFFFQ010000201         1284 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000201
VERSION     DAFFFQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.212"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(10..1197)
                     /locus_tag="LQB69_003020"
     CDS             complement(10..1197)
                     /locus_tag="LQB69_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL3527114.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          374 a          276 c          216 g          418 t
ORIGIN      
        1 ttcatggtgc taaatgttat tcgaattaaa ttctaaatat tttcgtggag aagccagcca
       61 ctcagtgtct ttttccatca aaagtgttcc aattatccga gtgatggaat tgatatttgg
      121 aaaaatccga atcactcgtt ctctccggcg aatctcttcg ttcaaccgtt ccaacacatt
      181 ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa tagcatcttc
      241 aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac catatttttc
      301 caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa agatactctt
      361 caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa tatttctcaa
      421 aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa cagccttaac
      481 taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc gttgaacgaa
      541 cgattgaaaa acggtatccc aattatcttc cgattcgccg tcttgaacat caaaaccaag
      601 aatcgtacgt cgtccatcag aatcaatgcc gataccaata tatacacctt ttgagacaat
      661 acgatgattt tcccttactt tcatatacag ggcatcacac atgaaaaatg gatattccga
      721 gccttctagg ctccggtttc tccactcaaa aacaaatgga tccaactgtt tcatcagatt
      781 tgaaacgaat gatttagaaa cagtcgcacc atccgttagc agttcaactg ttttagttac
      841 tttgcgagtg gagacaccag aaatgaccat ttcaatcatg gcagcgatca aagctttttc
      901 gcttcgctga tatctttcaa agatagtagg actaaactta ccgtctctgg ttcgtggaac
      961 atgaagttct aacgtaccaa ttcgagttgt aaacgaacga tcgtagtaac catttctttg
     1021 tgattgccgt tcttctgaac gttcataggc atttgcctga atatattgat ttcgttgttc
     1081 ttccatcagc tgattaaaca tgatagtcat aagtgttttt gcggtatcat tcgcaccgct
     1141 ttcgtttata attgcttgaa tttcttcgct ttcaaaagta aaatgtactt gagtcattga
     1201 tatcagtcct attcgagtat gttttctgca aaaaacattg tactcctaac tgagatcaat
     1261 ggcttttttg atgcttcact ttta
//
LOCUS       DAFFFQ010000202         1276 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000202
VERSION     DAFFFQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.213"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            35..532
                     /locus_tag="LQB69_003021"
     CDS             35..532
                     /locus_tag="LQB69_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL3527115.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="LQB69_003022"
     CDS             complement(569..1252)
                     /locus_tag="LQB69_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527116.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          217 c          224 g          391 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DAFFFQ010000203         1231 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000203
VERSION     DAFFFQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.214"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>1231
                     /locus_tag="LQB69_003023"
     rRNA            <1..>1231
                     /locus_tag="LQB69_003023"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DAFFFQ010000204         1215 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000204
VERSION     DAFFFQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.215"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            414..947
                     /locus_tag="LQB69_003024"
     CDS             414..947
                     /locus_tag="LQB69_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL3527117.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAFFFQ010000205         1213 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000205
VERSION     DAFFFQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.216"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            25..1203
                     /locus_tag="LQB69_003025"
     CDS             25..1203
                     /locus_tag="LQB69_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL3527118.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT          445 a          225 c          200 g          343 t
ORIGIN      
        1 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
       61 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      121 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      181 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      241 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
      301 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
      361 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
      421 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
      481 ttaaatgata agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa
      541 accgtatcca aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa
      601 gtactgagtt atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag
      661 gatattaact ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc
      721 atgaaagata ctatccatca aatttatcct aaagcaaaat atcagcattg ttgtatccat
      781 gtatctcgta acatcgctca taaagtacgt gtcaaagacc gaaaagaaat ctgtgatgac
      841 tttaaggctg tttatcaagc taactcaaaa gaagaagcga ataccttctt atccggcatg
      901 attgagaaat ggaagaaaaa ctatcctaaa gtgacgcagt cactcataga aaaccaagac
      961 ttattaactt tttatgattt tccacctagc attcgtagaa ccatttactc aaccaatcta
     1021 atcgagtctt tcaataagca aattaaaaga tacagccgta gaaaagagca gtttcaaaat
     1081 gaagaatcac tagaacgctt tctagtcagc atttttgata catacaatca aaaatttcta
     1141 aacagaagcc ataaaggttt tcaacaggta accgatacat tagtttcaat gtttactgag
     1201 taactaatta ttt
//
LOCUS       DAFFFQ010000206         1197 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000206
VERSION     DAFFFQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.217"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..153
                     /locus_tag="LQB69_003026"
     CDS             <1..153
                     /locus_tag="LQB69_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3527119.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="LQB69_003027"
     CDS             159..575
                     /locus_tag="LQB69_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527120.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="LQB69_003028"
     CDS             681..944
                     /locus_tag="LQB69_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL3527121.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="LQB69_003029"
     CDS             944..>1197
                     /locus_tag="LQB69_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL3527122.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          153 c          243 g          346 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga tttgaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacat atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DAFFFQ010000207         1193 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.218, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000207
VERSION     DAFFFQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.218"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1076
                     /locus_tag="LQB69_003030"
     CDS             <1..1076
                     /locus_tag="LQB69_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3527123.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFFFQ010000208         1173 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000208
VERSION     DAFFFQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.219"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            31..291
                     /locus_tag="LQB69_003031"
     CDS             31..291
                     /locus_tag="LQB69_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527124.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(653..892)
                     /locus_tag="LQB69_003032"
     CDS             complement(653..892)
                     /locus_tag="LQB69_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527125.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          416 a          164 c          218 g          375 t
ORIGIN      
        1 taatggaaaa acattaagga tggatgaaat atgaataacg aaactatcat tttaggaaaa
       61 aaatatattt gccaacctat tggcttgaaa caaaaagtgg ttggagaagt aatcagtaaa
      121 ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg acgtcgatcg caatgaaata
      181 ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg tttatgagtt ggaaacaaca
      241 gaatatctat ataactatag caacaaatat gaacatactt tagtttcata aagatttaaa
      301 taaagtgcct tcaaaagcga gaagaccaag cttttgaagg cactttattt agttggcagt
      361 gattgttcat gatttttgat ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg
      421 aggcctggtg gagaaactcg ataaaaacct atactgccag aactgtccta ttttcggata
      481 gacaaactag tagttttaaa aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg
      541 atatcaggat catttagctc aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag
      601 ttaggagtaa acgcgaaact atcatcatta tttgctgaag aagatgaaga agctaattca
      661 gagtttgttt gaaataaagg agacgtgctg acagtttctt taccctgttt tttgcagagg
      721 tagaaacttt tatatcggaa gtttctaccg gagaattttt ctcatttgta gaaattgttg
      781 aatcgatact attcgaaatt tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac
      841 aagcagtaag taacaataaa tttgataaac tgataaacag aattcttttc attatgtaca
      901 cactccttat agtatcgttt agcatacacc tatgctcagt attaggcaaa atcaatgcaa
      961 aactatcaag tcttttatag tatctattag tcttagtaat acatatatgg agagggtgat
     1021 atcccagttc aaaccagata cattggttac catctgtatt aacgattatg cctctaaacg
     1081 ctttttttat gaaaaaattt ataagtgaaa acgtaggtat gatattatct agaggtaatt
     1141 tttttgggta tttaaatagt tgctaggagg ata
//
LOCUS       DAFFFQ010000209         1162 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000209
VERSION     DAFFFQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.220"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            191..445
                     /locus_tag="LQB69_003033"
     CDS             191..445
                     /locus_tag="LQB69_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3527126.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="LQB69_003034"
     CDS             446..763
                     /locus_tag="LQB69_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3527127.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          411 a          176 c          231 g          344 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tg
//
LOCUS       DAFFFQ010000210         1115 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000210
VERSION     DAFFFQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.221"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            2..400
                     /locus_tag="LQB69_003035"
     CDS             2..400
                     /locus_tag="LQB69_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL3527128.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LQB69_003036"
     CDS             384..1079
                     /locus_tag="LQB69_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL3527129.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          429 a          163 c          237 g          286 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcg
//
LOCUS       DAFFFQ010000211         1100 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000211
VERSION     DAFFFQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.222"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..557)
                     /locus_tag="LQB69_003037"
     CDS             complement(<1..557)
                     /locus_tag="LQB69_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL3527130.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGI"
     gene            complement(574..978)
                     /gene="tnpA"
                     /locus_tag="LQB69_003038"
     CDS             complement(574..978)
                     /gene="tnpA"
                     /locus_tag="LQB69_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL3527131.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          335 a          182 c          178 g          405 t
ORIGIN      
        1 tcaataccaa ttgtggaatt agttttaggt aattcgataa tttcttcttt acacaacaaa
       61 gaaatatagt atttaccgct agaatgacgt gatattgtag cagatttgat aatccctttt
      121 ggttgtctat gaagcttaat tcttactaat gacttcaatt taggaacttt gatgaattta
      181 ctatcaatca aagcaattgt accattttga ttattcgttg tataactctg aacaggattt
      241 ttcttacttt tgaaacgtgg aaatccaacg gatttatccc gaaaaaaatt cttgtatgct
      301 ttatctaaat taagttgggc attggctaaa gcaaggctat caatttcttt caaaaacgga
      361 aattctttct tgtatttagc tggtgtcgga aaagtcattt ttttagaaga atcattttta
      421 acttcttcat acgccttttt tcggtcatca agcattagat tgtagacctt acggacacaa
      481 ccaaaagatt tggcaaagaa gatttcttgt tcctctgttg gatagattct gaatttgtat
      541 gcttttagtt gttccataaa gttcacctac tttctattta tgattgtttc cttgattttg
      601 gatatacttc ttaatcacat caattggtgc gccaccagtt gtcagaagac aaaagctttt
      661 agaccagaac atttctttcc aaagaaattg tttgactctt ggaaagtcac gttttatcaa
      721 tcttgagctg gcacttttat aggcattgat gaattttgtc aattctgttt taggttgagc
      781 tttgaacata atatgaacgt ggtctttatc atgattccac tccactaaag taatgtgata
      841 cgattctgaa attctttcaa aagtagtttt ggcataatca gatatttcat catcaatcac
      901 ttgtctacga tatttcgtta ctaaaacaag gtggtaataa agaagaaata ctgaatgatt
      961 atttgtatct aattccatgt tatatcaact catttcttgt atagactgat tgtaacatgg
     1021 gacaaaataa aaaggcaata cgccttagtt gtcggtcttc gagttaccaa cggtaaccct
     1081 cataccgaac ggcattcatc
//
LOCUS       DAFFFQ010000212         1085 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000212
VERSION     DAFFFQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.223"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            2..>1085
                     /locus_tag="LQB69_003039"
     rRNA            2..>1085
                     /locus_tag="LQB69_003039"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DAFFFQ010000213         1083 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000213
VERSION     DAFFFQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.224"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..276
                     /locus_tag="LQB69_003040"
     CDS             <1..276
                     /locus_tag="LQB69_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL3527132.1"
                     /translation="RNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQ
                     GGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            619..875
                     /locus_tag="LQB69_003041"
                     /pseudo
     CDS             619..875
                     /locus_tag="LQB69_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          375 a          164 c          198 g          346 t
ORIGIN      
        1 cgtaatccct tcgtatctga atatgttaaa cgacttgcaa aaggtatttg tcaactttgc
       61 caagaaaaag ggccatttat aaaagacggt gtaccttatt tacattgtca tcatatagaa
      121 tatttgtcgc aaggtggaaa agatgtgatt gaaaactgta tcgcgttatg tcccaattgt
      181 catgctagaa ttcatgaact tgagttacct gacgataaag aaaaattgct gaagatcgtt
      241 gaaaaaagag aaaatagctt ttctaaaata ccgtaattta tttttatagg acaaataagc
      301 taatgtgagc tatgccttct atataagact aagttctatt ataagtaacg aagtaattct
      361 cgttccttcc tgtagtaata ttttttaaat aacctatagg cttcttcaaa agtacatcct
      421 ccagtaaatt ctaagtactg gaggattctt tttctagtgt tgctttactc atgaaaaagt
      481 agttggctac ttcatcaaaa tttctgaact tttttaatgt ataataaaga gctcgatcat
      541 ttactacaag gatgctagct cctacatttt cacagttttt tagcaagtaa atttttctaa
      601 aatcacatca ggaaatgcat gagattcttt aagaaaggca attatagggc tatgattacg
      661 gttcgtgtaa gtgacgccaa aaaagaatgg ctttcttata tggctgaatt ttatgggatt
      721 actttatctg aacttctaaa aaactattcg atggaacaac tagaagatga atacaatcgt
      781 caaacggctg aaattgccca taaacattgg ataagaagtg ggaaaacaac agtattcatg
      841 gaagaatcct taatgaattt ggtgggctgg aatgacctgc cgtttaaaat tcacgcccga
      901 aattcagaag cagctctgat tggcaaatac gtgtattggt agagatagaa gataatcaat
      961 tcgttgttac cgctattcaa gtagggcacc gaagaaatgt ctatgattaa gagactgttt
     1021 atttttacaa aaaaataata actggaatct agttaaaact cttgaagtga gagatttgac
     1081 tag
//
LOCUS       DAFFFQ010000214         1069 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000214
VERSION     DAFFFQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.225"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..969
                     /locus_tag="LQB69_003042"
     CDS             <1..969
                     /locus_tag="LQB69_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL3527133.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAFFFQ010000215         1062 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000215
VERSION     DAFFFQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.226"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            4..963
                     /locus_tag="LQB69_003043"
     CDS             4..963
                     /locus_tag="LQB69_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL3527134.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          366 a          195 c          212 g          289 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DAFFFQ010000216         1058 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000216
VERSION     DAFFFQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.227"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(36..992)
                     /locus_tag="LQB69_003044"
     CDS             complement(36..992)
                     /locus_tag="LQB69_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527135.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DAFFFQ010000217         1051 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000217
VERSION     DAFFFQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.228"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="LQB69_003045"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="LQB69_003045"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL3527136.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAFFFQ010000218         1002 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000218
VERSION     DAFFFQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.229"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            52..307
                     /locus_tag="LQB69_003046"
                     /pseudo
     CDS             52..307
                     /locus_tag="LQB69_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            371..1002
                     /locus_tag="LQB69_003047"
                     /pseudo
     CDS             371..1002
                     /locus_tag="LQB69_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          344 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaaaa
      301 tataggctct ataatttatg ggactgatga atcaaaattg attcttcagt cccattgtta
      361 ttttaggtat atgattcgta cattgaaaga gttgaaaaac tatcttccga agccgtcaaa
      421 aaggaccctg ccaaaagtat tgatggtgga tgaatttcgt tcgcacgctt caattgtgga
      481 taaaatgagc tttatttgtg cggatggcga gactggaaaa ctcattgata ttttgcctac
      541 acgaaaattg tctcgattaa cacactattt tagtcgctgt gcaaatcctg aagcggtgga
      601 atatttagtg acagatatga acgccgccta cttccaactg acaaaacgtg tcttaccaaa
      661 tgcgaaagtc gtcattgatt gttttcatat catcaaacat ttgaatcagg cgttcaatga
      721 ttttcgtgta caagaaatga aaagattaca ccagttaggg caacgacggg aagccgagaa
      781 agtgaaaaag aactggcgct ttcttctaaa aaaccgtcaa aaaatcaatc actatgatta
      841 caaaacatgg cgcagttttc gtgcacctaa atacccgcta ctcactgaag caatgatgat
      901 tgatcgattg ctggcatttt caacgccttt aaaggacgcc tatacttttt tccatgagct
      961 gaccgaagcc tttagagaca aggaatcaga cttatttttt ac
//
LOCUS       DAFFFQ010000219          991 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000219
VERSION     DAFFFQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.230"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            52..>991
                     /locus_tag="LQB69_003048"
     CDS             52..>991
                     /locus_tag="LQB69_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3527137.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKD"
BASE COUNT          355 a          180 c          190 g          266 t
ORIGIN      
        1 gtgctacact caaaatagtt ccgacgaaag aattataagg agtgagtaca aatgacctat
       61 aaccatctta caccaactga actcgtaatg atagaagctt atttcaatca atcacaacct
      121 gtttcaaaag tagccaatct gttacaacgt tctcgacaaa ctatttataa ggtctatcgt
      181 tttcttaaat ctggtggaag cgcaatgagc tactataaac aatataagaa aaacaaacgt
      241 aactgcggaa gacatccgat tgttttacct gatgagcaac aggaatacat tcaaaaaaag
      301 gttgttcaag ggtgggctcc tgatgtcctt attggtcgag cagagtttcc tattgcttgt
      361 tcggtacgca cactgtatcg aatgtttaaa gataaaacct ttgacactca cgatttacca
      421 atgaagggaa aaagaaaacc aaacggtcat aaagaaaaaa gaggaaaaca agggtttcgc
      481 cggtcaatcc gagacagaaa gacagactat acccagtttg atcatgaatt tggtcacctg
      541 gaaggtgaca ctatcgtggg tggaaaacat aaaagcgcgg tcatcacatt agttgaacgg
      601 ctatctaaag tcatcattac cttgaaaccc gaaggcagac aggctaaaga cattgaaaat
      661 aggctgaatc aatggtttcg ttgtgtccca aaaaacttat ttaaatccat cacatttgat
      721 tgtggaaaag aattctcaaa ttggaaaaag attagtaata ccaacgatat ttcaatttat
      781 tttgcggatc caggaacacc ttctcaacga ggattgaatg aacattcaaa tggcttatta
      841 cgtaaagatg gtttgtataa agctatggat tttaatggtg tatctgaaac ctttatccaa
      901 tcggtggcat cgaaacgaaa tcatattcct agaaaatcat tacactatcg gacaccaatg
      961 gaagtatttt tgagttacgt cacaaaagat g
//
LOCUS       DAFFFQ010000220          952 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000220
VERSION     DAFFFQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.231"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>952)
                     /locus_tag="LQB69_003049"
     rRNA            complement(<1..>952)
                     /locus_tag="LQB69_003049"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a          300 c          211 g          243 t
ORIGIN      
        1 cgtcatcccc accttcctcc ggtttgtcac cggcagtctt gctagagtgc ccaactgaat
       61 gatggcaact aacaataagg gttgcgctcg ttgcgggact taacccaaca tctcacgaca
      121 cgagctgacg acaaccatgc accacctgtc actttgcccc cgaaggggaa gctctatctc
      181 tagagtggtc aaaggatgtc aagacctggt aaggttcttc gcgttgcttc gaattaaacc
      241 acatgctcca ccgcttgtgc gggcccccgt caattccttt gagtttcaac cttgcggtcg
      301 tactccccag gcggagtgct taatgcgtta gctgcagcac tgaagggcgg aaaccctcca
      361 acacttagca ctcatcgttt acggcgtgga ctaccagggt atctaatcct gtttgctccc
      421 cacgctttcg agcctcagcg tcagttacag accagagagc cgccttcgcc actggtgttc
      481 ctccatatat ctacgcattt caccgctaca catggaattc cactctcctc ttctgcactc
      541 aagtctccca gtttccaatg accctccccg gttgagccgg gggctttcac atcagactta
      601 agaaaccgcc tgcgctcgct ttacgcccaa taaatccgga caacgcttgc cacctacgta
      661 ttaccgcggc tgctggcacg tagttagccg tggctttctg gttagatacc gtcaagggat
      721 gaacagttac tctcatcctt gttcttctct aacaacagag ttttacgatc cgaaaacctt
      781 cttcactcac gcggcgttgc tcggtcagac tttcgtccat tgccgaagat tccctactgc
      841 tgcctcccgt aggagtttgg gccgtgtctc agtcccaatg tggccgatca ccctctcagg
      901 tcggctatgc atcgtggcct tggtgagccg ttacctcacc aactagctaa tg
//
LOCUS       DAFFFQ010000221          920 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000221
VERSION     DAFFFQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.232"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..535
                     /locus_tag="LQB69_003050"
     CDS             <1..535
                     /locus_tag="LQB69_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL3527138.1"
                     /translation="TFFEYLKERGLQGTELVISDAHKGLVSAIRKSFTNASWQRCQVH
                     FLRNIFTTIPKKNSKSFREAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLD
                     DGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNCVSANRLIGAVLID
                     QHDEWLSSSRKYIKFAK"
BASE COUNT          311 a          161 c          136 g          312 t
ORIGIN      
        1 aacatttttt gaatacctaa aagaacgcgg tttacaaggt acagaactcg ttatttctga
       61 tgcgcacaaa ggattagtct ctgccattag aaaatccttc accaacgcaa gttggcaaag
      121 atgccaagtt cacttcctaa gaaatatctt taccaccatt cctaaaaaaa attcaaaatc
      181 tttcagagaa gctgttaaag gaatttttaa gttcacagat attaacttag ctcgtgaggc
      241 taaaaatcga ttgattcatg attatatcga tcaaccaaaa tattcaaaag cttgcgcatc
      301 attggatgat ggattcgaag acgcctttca atataccgta caaggaaatt cccacaatcg
      361 actaaagagt accaatctaa ttgaacgact gaatcaagaa gtgcgcagaa gagaaaaaat
      421 tattcgtatc tttccaaact gcgtttcagc caatcgttta attggagcag tccttattga
      481 tcaacacgat gagtggcttt cttcatcaag aaaatatatt aaatttgcca agtaaaaaat
      541 agtgagagca ttgtatagta ttttacacag gattgtggac ttgacttttt tttcatttat
      601 tatctattat tctcttttaa aaatagcccc aatgatctta tgtaagtaaa cggacggaca
      661 atttataata tagcactttg cttcttgttg cttgtatggt tatttttgtg tttaaaatct
      721 aaaagtctaa ctaaaattct acctttaaat ttcagattct tgaatgccca atttatatag
      781 agcactatca tgcttctttt gttcttggac gcaatttctt ctggtctatt cattcaatcc
      841 tagtgtattt acacaatatt cctttggcct aaaactttga taaatacgta tatataaatt
      901 tacgtattta tcaaaaatat
//
LOCUS       DAFFFQ010000222          862 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000222
VERSION     DAFFFQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.233"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            31..>862
                     /locus_tag="LQB69_003051"
     CDS             31..>862
                     /locus_tag="LQB69_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3527139.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          298 a          150 c          166 g          248 t
ORIGIN      
        1 cgaccaaaga attataagga gtgagtacaa atgacctata cccatcttac aacggatgaa
       61 cttgtaataa tagagtctta tttcaaaatg aatcaatctg ttgctaaaac tgcccattgc
      121 ttgaatcgtt caagacaaac gatccataaa gtatatctgt tcttcaagca aggaaaatca
      181 gccttagaat attatcaaca gtataagaaa aacaaatcaa actgtggtag acgtccgctt
      241 gttttacccg aggaacaatc agaatatatt caaagaaagg ttgttcaagg atggacaccc
      301 gatgtgattg ttggtcgtgc agcgtttcct attagttgtt ctgctcgtac catttatcgt
      361 atgttcaaaa aggggctatt tgattcttct gacttaccga tgaaaggcaa gcgtaaaccg
      421 aatggacatc aagaaagacg tggaaaacaa actttccgcc gctctattca tgaacgtgaa
      481 aaggattata gccaattctc aaatgagttt ggtcaccttg aaggtgacac tatcgtaggt
      541 ctgaaacata aaagtgctgt aattacctta gttgaacgat tatcaaaagt tatcatcaca
      601 ttgaaaccgt gtggtagaca agcgattgat attgaaaaaa aattaaatca atggtttgaa
      661 tctgtaccga aaaacctatt caaatccatc acttttgatt gtggaaagga attttcaaat
      721 tggaaacaga tcagtaatgt caatgatatt gccatttatt tcgctgatcc aggaacgccg
      781 tctcaaagag gcctaaacga gaattctaac ggattgttac gtagagatgg tttattgaaa
      841 tctatggatt tcaattcagt ag
//
LOCUS       DAFFFQ010000223          841 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000223
VERSION     DAFFFQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.234"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..146)
                     /locus_tag="LQB69_003052"
     CDS             complement(<1..146)
                     /locus_tag="LQB69_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527140.1"
                     /translation="METTDTLLQLLQEAHKTNQTQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGR"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LQB69_003053"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="LQB69_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL3527141.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="LQB69_003054"
     CDS             complement(591..785)
                     /locus_tag="LQB69_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527142.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          244 a          167 c          146 g          284 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgtt tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 t
//
LOCUS       DAFFFQ010000224          791 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000224
VERSION     DAFFFQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.235"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..782
                     /locus_tag="LQB69_003055"
     CDS             <1..782
                     /locus_tag="LQB69_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL3527143.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DAFFFQ010000225          775 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000225
VERSION     DAFFFQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.236"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(249..728)
                     /locus_tag="LQB69_003056"
     CDS             complement(249..728)
                     /locus_tag="LQB69_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527144.1"
                     /translation="MCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNSL
                     DFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKCFESAVVKFPQFKLNP
                     AASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DAFFFQ010000226          755 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000226
VERSION     DAFFFQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.237"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..104
                     /locus_tag="LQB69_003057"
     CDS             <1..104
                     /locus_tag="LQB69_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL3527145.1"
                     /translation="ETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            104..721
                     /locus_tag="LQB69_003058"
     CDS             104..721
                     /locus_tag="LQB69_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527146.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          283 a           95 c          162 g          215 t
ORIGIN      
        1 ttgaaacagt agaatttcca attgaagaag gagctttgat tattgcaact gttgatgatc
       61 caaaacagaa tgttttcact cttgagaaag ttggggtgat ttgatggtta aagtaaagag
      121 aatgatggag actgatgaat ctggtgttaa acgtcaattt atgccaatta cacatgtttc
      181 tgcagtttta ggcttagaaa aaatgatatc aggacaatca aaagttcttt cggttaatgg
      241 taaatatggt gcagtcattt taacgaaagc tgatttagga ttagagaatg caattactga
      301 actaccttat gcaagcgaga caagcgatgg tattcttact gctgaaatgt ttcaaaagat
      361 cgtaaatgga gaaggtggaa catatatttt gccgattgct actcctgaac aattaggagg
      421 tataaaagtt ggggaactct tagaagttac tgaagaaggt gttttatctg caactaagca
      481 aacagacttc aatttctctg aagaattaaa gggaaaatta gaatctctaa aaatattaaa
      541 agcaggtgca aatatatcaa ttgcagaaga tggaacaatt agttctacag gtggtagtgg
      601 gactggtggt gttaatcagt catatgtaga tcaaaaattc caagaagctg taaatcaagc
      661 agaaaattac acaaacgaaa gaattccaaa ttttactttt gaaaaaatcg gggaggtata
      721 gaaaatgaca gatatcgtaa aaataaaaca aagcg
//
LOCUS       DAFFFQ010000227          753 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000227
VERSION     DAFFFQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.238"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            363..731
                     /locus_tag="LQB69_003059"
     CDS             363..731
                     /locus_tag="LQB69_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL3527147.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          244 a          117 c          157 g          235 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt ttt
//
LOCUS       DAFFFQ010000228          740 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000228
VERSION     DAFFFQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.239"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            176..379
                     /locus_tag="LQB69_003060"
     CDS             176..379
                     /locus_tag="LQB69_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HBL3527148.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            379..729
                     /locus_tag="LQB69_003061"
     CDS             379..729
                     /locus_tag="LQB69_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL3527149.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          299 a           79 c          119 g          243 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttacactct cgtcaatcat
       61 tgttatataa taaaatgtta tataataaaa ttactaatga tatttaatat tacaagcaat
      121 cttaagaaat tcgaaaagga aaaaatgatt atgaaaaaga aagaaaaaga aagtattgaa
      181 acattgtgtt attctaggaa tattaggaac ttgtctagct ggcattggta caggaataga
      241 cgttgacgca gctacttact atggaaatgg tctttattgt aacaaagaaa aatgttgggt
      301 aaattgggga caatcatggt cagaagggtt aaaacgttgg ggagataatc tttttggtag
      361 ttttagtgga ggacgataat gacttcaata aacattgatc acacaaaaaa agtcatacat
      421 gaattataca atagtttaat gacacgacct aatcagacga aagcattatt agatattact
      481 gatgttttat ttcaggtata taaaaaaatt gatacagcaa aatatcctga aattcttgta
      541 aataaattag ttaattatat ttacatcgtg ggttttgata ataaaatacg ctttattgat
      601 aatgatgaaa aattattaat agaattaggg gatatttcta aaaaagctgg aattaatagt
      661 aaatacaaag caaactttac agataaatcg caattttata gttattcaga aacaatgcct
      721 caacgataaa atatagtata
//
LOCUS       DAFFFQ010000229          705 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000229
VERSION     DAFFFQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.240"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..258
                     /locus_tag="LQB69_003062"
     CDS             <1..258
                     /locus_tag="LQB69_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527150.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="LQB69_003063"
     CDS             260..397
                     /locus_tag="LQB69_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL3527151.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..>705
                     /locus_tag="LQB69_003064"
     CDS             434..>705
                     /locus_tag="LQB69_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527152.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT          279 a           98 c          138 g          190 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa acttt
//
LOCUS       DAFFFQ010000230          695 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000230
VERSION     DAFFFQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.241"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          247 a          115 c           85 g          248 t
ORIGIN      
        1 caaattactt catcaaaaca ttgatataat ttttattata tcagtattta ttcttgatac
       61 ataaatgaga aaataaatac tttaaatcta ttgactacaa gtagtcatta agtcttcata
      121 agatgtacaa tatgacatta aaaaaataaa agcttacgac catccgttaa atctgacact
      181 aaataatgga ctgctttctt ttgtattagc aggttacgtt gactatgaat attcaccaga
      241 ttatttggag aaacctcttc aacacgagaa caatgatatt aattttagat ttgtctacga
      301 gtcaaaaagt aaaacatggt tagttgatca attaagcacg ataaacacaa gcttccatga
      361 attacctaaa cctgaaaatg aagttgagat ggtcattaaa atcttctaaa aaagaagcgt
      421 tgaaggggaa atattccttt caacgctctt ttttatatgc ttagaaacat aaataaattg
      481 tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttt cgacgctagc
      541 gtttctctac ttctaatact gtacacagtt tttgtattat tgaaaaatta ctcattattt
      601 agcattaatc cacatcgttt tatcaatcga ccttcatact aaactacttt aaagtcatct
      661 tccttctcgt ttttcattaa tggaatggca acagt
//
LOCUS       DAFFFQ010000231          680 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000231
VERSION     DAFFFQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.242"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..549
                     /locus_tag="LQB69_003065"
     CDS             <1..549
                     /locus_tag="LQB69_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527153.1"
                     /translation="IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQ
                     KRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSW
                     SKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHD
                     RDINASINILTEGLRLHSMGLA"
BASE COUNT          254 a          109 c          129 g          188 t
ORIGIN      
        1 attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga acaacgcaaa
       61 ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga agctaaaaat
      121 tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa tcaacgtact
      181 gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat ctgtattgaa
      241 gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat ttctgatgtt
      301 tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg acgaaaaatc
      361 atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg acataaagat
      421 ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac tcatcatgat
      481 cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca ttctatggga
      541 ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg gtaaataaga
      601 gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa gaagctccca
      661 cttcaagcgt taagaaccgt
//
LOCUS       DAFFFQ010000232          637 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000232
VERSION     DAFFFQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.243"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            74..403
                     /locus_tag="LQB69_003066"
     CDS             74..403
                     /locus_tag="LQB69_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3527154.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..>637
                     /locus_tag="LQB69_003067"
     CDS             393..>637
                     /locus_tag="LQB69_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL3527155.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          244 a           70 c          123 g          200 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtgg
//
LOCUS       DAFFFQ010000233          615 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000233
VERSION     DAFFFQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.244"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          180 a          139 c           97 g          199 t
ORIGIN      
        1 attttgttcc tcctcaaaga aaaagagact tttggaaaga atttccagag ttcacgtaac
       61 cctaaaaatt catatccaaa aatctctagc ttgaagaaac ctaaaaatat tcgtataatg
      121 aacctgagtt acggagctag gtgaagtagt ttccgtaaat agtcgagagt gttagcgcac
      181 tgctcggctt ttattttgct cttttttctc gttttctttg attatagcac tcctcctaaa
      241 aaaataaaaa gacctcaggg aatttcattc cccgaacccc ttgagcctcc tcctgccgaa
      301 atggaaaggg cgcacccgct gtttatttca ttcagctatc ggctttcaca atctaacacg
      361 atcgttccgc taccagtcgc cttacaggct ccattttcgc tcctctaacg tgttttaacg
      421 attgtgccga taactaaacg aaataaacgc taaaaccgct cagaacgcaa aatatgccat
      481 tttagagcta ttgtagaaaa tgtagtttga aatgcaattt tcttctttca gctttttatt
      541 tcccacttgt aaatctgcta attattttct accagacaaa ccattgattt tttggctatc
      601 agttaactca cgatt
//
LOCUS       DAFFFQ010000234          614 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000234
VERSION     DAFFFQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.245"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..308)
                     /locus_tag="LQB69_003068"
     CDS             complement(<1..308)
                     /locus_tag="LQB69_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3527156.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          195 a          119 c           86 g          214 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgccc
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca cgtcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataaggaa
      421 aaggtagtac ataaataagg aaaagctgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aattttttca actg
//
LOCUS       DAFFFQ010000235          614 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000235
VERSION     DAFFFQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.246"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..308)
                     /locus_tag="LQB69_003069"
     CDS             complement(<1..308)
                     /locus_tag="LQB69_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL3527157.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          197 a          117 c           83 g          217 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccaattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataagtaa
      421 aaggtagtac ataaataagg aaaagttgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aattttttca actg
//
LOCUS       DAFFFQ010000236          588 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000236
VERSION     DAFFFQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.247"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          192 a           83 c           83 g          230 t
ORIGIN      
        1 aaattataga gccatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaac
       61 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
      121 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      181 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      241 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      301 ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga gtaattaatt
      361 agatgtttat tttataggac tgatactata gattacaaat agtatcgtat ttcgcaatct
      421 taaaattatc ttcttactgt gaataagata ataaaatttg tatttgtcct ttttaggtaa
      481 cttattttaa tctatatacc tttctggatc aacgaaagta tactttatat agtcataacg
      541 ccgatggtaa ctatgcactg taacgataat gctgctaaaa tatctttt
//
LOCUS       DAFFFQ010000237          578 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000237
VERSION     DAFFFQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.248"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            126..413
                     /locus_tag="LQB69_003070"
                     /pseudo
     CDS             126..413
                     /locus_tag="LQB69_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="LQB69_003071"
     CDS             427..>578
                     /locus_tag="LQB69_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527158.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAFFFQ010000238          563 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000238
VERSION     DAFFFQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.249"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..129
                     /locus_tag="LQB69_003072"
     CDS             <1..129
                     /locus_tag="LQB69_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3527159.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..>563
                     /locus_tag="LQB69_003073"
     CDS             130..>563
                     /locus_tag="LQB69_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527160.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYP"
BASE COUNT          233 a           80 c          119 g          131 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tcc
//
LOCUS       DAFFFQ010000239          561 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000239
VERSION     DAFFFQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.250"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            298..>561
                     /locus_tag="LQB69_003074"
     CDS             298..>561
                     /locus_tag="LQB69_003074"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL3527161.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          192 a          101 c           82 g          186 t
ORIGIN      
        1 ttattctttt cataaatatt gtaaatagaa gtattaaaaa tatatacaaa aagtattatt
       61 tcgagaaaga taaatatttt caaaaacctt ttcataatcc tcaccctttc ttacactgat
      121 aaatcatgcg tctaatattc aaattatact cgatatgctt tagctaatca gccaactctg
      181 tttgtctact atcattatat caatagacaa tatctgctcc cgtctttatg atcgtgccaa
      241 agcaatcaaa agaaaattgc gaatcactta gatcgaaagt ttactcaaac aaaagcattg
      301 tcagcggttg tcacggattt aacttatgtt cgggtcggga caaattgggc ttatatttgt
      361 ttgatgattg atttattcaa tcgtgaaatt attggccatt cttgcggaaa taaaaaagat
      421 gcgcagcttg taaaaagggc cattcaaagc attccttact cacttcaaga aatcgaactc
      481 ttccatacgg atcgtggaaa agaatttgat aaccaaacca ttcagaatct catgaatggt
      541 ttggttatca aattcctttc c
//
LOCUS       DAFFFQ010000240          546 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000240
VERSION     DAFFFQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.251"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(271..534)
                     /locus_tag="LQB69_003075"
     CDS             complement(271..534)
                     /locus_tag="LQB69_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HBL3527162.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          213 a           97 c           75 g          161 t
ORIGIN      
        1 actctcctga attttttcat gttgaaaaca tcactaatca aaggcaaatt ataatttaga
       61 aatagaatga aaacactaaa tgaaaataga acattagaaa aaatagaatc aaaaaaaccc
      121 aagactagtg ccgaaataag ggaaacaatc gaagctacaa aaaataatga tttcgttgaa
      181 caggaccaat ttatttgttt ccgatatttc tcaaaaagaa ctttctttgg gaaagttaaa
      241 ggcgactgta aagtcgcctt tagtcctttt ttaccaagct gctgcatatt tagctccata
      301 ttttttaatt aatgatttgg ctgtagcaac aataccagct gtaattaatc cgcctccaac
      361 aactgcagca acaatagaaa tgattgtagc aaccgtgcta ccagtattaa ttatgtcaat
      421 aactttttta gctgcataag aagaaattcc aatgttagca cttagcattg gtacaccaac
      481 aaagaatccc gcaaaaccta aaattggtaa aactaataat aaattctttt tcataataat
      541 tcctct
//
LOCUS       DAFFFQ010000241          528 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000241
VERSION     DAFFFQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.252"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(5..90)
                     /locus_tag="LQB69_003076"
     tRNA            complement(5..90)
                     /locus_tag="LQB69_003076"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="LQB69_003077"
     tRNA            complement(107..178)
                     /locus_tag="LQB69_003077"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="LQB69_003078"
     tRNA            complement(190..262)
                     /locus_tag="LQB69_003078"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="LQB69_003079"
     tRNA            complement(280..361)
                     /locus_tag="LQB69_003079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="LQB69_003080"
     tRNA            complement(369..441)
                     /locus_tag="LQB69_003080"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="LQB69_003081"
     tRNA            complement(445..517)
                     /locus_tag="LQB69_003081"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAFFFQ010000242          509 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000242
VERSION     DAFFFQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.253"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..132)
                     /locus_tag="LQB69_003082"
     CDS             complement(<1..132)
                     /locus_tag="LQB69_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL3527163.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAFFFQ010000243          504 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000243
VERSION     DAFFFQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.254"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DAFFFQ010000244          490 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000244
VERSION     DAFFFQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.255"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          161 a           94 c           75 g          160 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat
//
LOCUS       DAFFFQ010000245          487 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000245
VERSION     DAFFFQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.256"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>487)
                     /locus_tag="LQB69_003083"
     CDS             complement(<1..>487)
                     /locus_tag="LQB69_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBL3527164.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAFFFQ010000246          482 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000246
VERSION     DAFFFQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.257"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..408)
                     /locus_tag="LQB69_003084"
     CDS             complement(<1..408)
                     /locus_tag="LQB69_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527165.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKP"
BASE COUNT          179 a           78 c           62 g          163 t
ORIGIN      
        1 cggctttcca aatgatgaaa tacctaaatc taacttatta agaaagttga aatttttata
       61 ttcaggaaac catttattaa tttcatgtgc agcacgtaat gcattccctg gagttgtcaa
      121 agagaaaatt aattctgcta agcttattat tgaaaatact gataacttat aattatatcg
      181 tccattgaca atcaaatata aactaactat acttgttaga acaaaaaaac aggcgttgac
      241 ttgttcttga ttagttaaaa atatcaataa aggaatactt acccaatata tttttctatt
      301 tatttcttct cctcttaaga gttgaaaaaa cggataaaaa gttagtaaac taaatgctac
      361 tggccactga tagttcaagg tggttgctct ccaaccggtt tcccccatta ctgtcaatgg
      421 tattaaacag tatatagagc aaaccaataa taaattttta acatttattt tctcaaaaac
      481 at
//
LOCUS       DAFFFQ010000247          481 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000247
VERSION     DAFFFQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.258"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>481
                     /locus_tag="LQB69_003085"
     CDS             <1..>481
                     /locus_tag="LQB69_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527166.1"
                     /translation="TFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     "
BASE COUNT          194 a           74 c          112 g          101 t
ORIGIN      
        1 taaccttcac cgcaggagaa ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga
       61 aagacggcgt aagcttacca gaagataaac ttccagtgtt gaaagcaaaa gatggttata
      121 cagatgcgaa atggccagag gaagcaactc aaccaattac agcagatgat acagaatttg
      181 tatcaagtgc aacaaaactg gatgataaat ctgacgctga caaatataat cctgaaggtc
      241 aaaaagtgac tacagaattg aataaggaac cagacgcatt tgagggaatt aagaacaaag
      301 aagatttacc aaaagatact aagtatactt ggaaagaaaa agtagatgtt agcgcagctg
      361 gaaataaaaa agggactgtt gtagtgacat atccagatgg atcatctgat gaagttgaag
      421 tagatgttac agtgacagac aatcgttctg acgccgataa atatgagcca acagtagaag
      481 g
//
LOCUS       DAFFFQ010000248          467 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000248
VERSION     DAFFFQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.259"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            1..467
                     /locus_tag="LQB69_003086"
                     /pseudo
     CDS             1..467
                     /locus_tag="LQB69_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          191 a           84 c           87 g          105 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcc
//
LOCUS       DAFFFQ010000249          460 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000249
VERSION     DAFFFQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.260"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(20..91)
                     /locus_tag="LQB69_003087"
     tRNA            complement(20..91)
                     /locus_tag="LQB69_003087"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="LQB69_003088"
     tRNA            complement(113..200)
                     /locus_tag="LQB69_003088"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="LQB69_003089"
     tRNA            complement(225..298)
                     /locus_tag="LQB69_003089"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="LQB69_003090"
     tRNA            complement(312..382)
                     /locus_tag="LQB69_003090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="LQB69_003091"
     tRNA            complement(384..459)
                     /locus_tag="LQB69_003091"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAFFFQ010000250          456 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000250
VERSION     DAFFFQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.261"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..397
                     /locus_tag="LQB69_003092"
     CDS             <1..397
                     /locus_tag="LQB69_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527167.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTTAGLMSATDKVKLDGLANITFEKVGTV"
BASE COUNT          161 a           78 c          106 g          111 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgacagcag gtttaatgtc agcaaccgat aaagtgaaac ttgatggatt
      361 agcgaatatt acatttgaga aagtggggac ggtttaatgg ctgatattgt tcaattaaaa
      421 gaagacggag ttgctaaata cttaaaaacg catgta
//
LOCUS       DAFFFQ010000251          430 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000251
VERSION     DAFFFQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.262"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>430
                     /locus_tag="LQB69_003093"
     CDS             <1..>430
                     /locus_tag="LQB69_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL3527168.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DAFFFQ010000252          409 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000252
VERSION     DAFFFQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.263"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..379
                     /locus_tag="LQB69_003094"
     CDS             <1..379
                     /locus_tag="LQB69_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBL3527169.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAFFFQ010000253          400 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.264, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000253
VERSION     DAFFFQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.264"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          141 a           80 c           64 g          115 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc cttacaaata aataataacc tttaacaacc
       61 aactcgtaaa taggttggtt gttattatac aaaaggatat tagtactcag ataaaaagtt
      121 gttagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt actcagataa
      181 aaagttgtta gtacccttct gtatccgttg ggagagtaag gctcaactgc cccctaaaaa
      241 gggttctaaa aaatctttaa aaaataaata aaataaacgc gcgcgcgcga gcaaaatttc
      301 ccgccaaaaa atcacccact ttttaaaata aacaggttcg cgcatgatat taaactgcca
      361 atctagctcc ctatggtcgc tgtaagaagg agtgatcgct
//
LOCUS       DAFFFQ010000254          394 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000254
VERSION     DAFFFQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.265"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..285
                     /locus_tag="LQB69_003095"
     CDS             <1..285
                     /locus_tag="LQB69_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3527170.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DAFFFQ010000255          381 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000255
VERSION     DAFFFQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.266"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(21..94)
                     /locus_tag="LQB69_003096"
     tRNA            complement(21..94)
                     /locus_tag="LQB69_003096"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="LQB69_003097"
     tRNA            complement(108..181)
                     /locus_tag="LQB69_003097"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="LQB69_003098"
     tRNA            complement(209..281)
                     /locus_tag="LQB69_003098"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="LQB69_003099"
     tRNA            complement(301..374)
                     /locus_tag="LQB69_003099"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAFFFQ010000256          361 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000256
VERSION     DAFFFQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.267"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DAFFFQ010000257          360 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000257
VERSION     DAFFFQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.268"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..233
                     /locus_tag="LQB69_003100"
     CDS             <1..233
                     /locus_tag="LQB69_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527171.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT          135 a           63 c           51 g          111 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
//
LOCUS       DAFFFQ010000258          350 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000258
VERSION     DAFFFQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.269"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>350
                     /locus_tag="LQB69_003101"
     CDS             <1..>350
                     /locus_tag="LQB69_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL3527172.1"
                     /translation="LSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQ
                     AKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEA
                     NLPNILGDKGYISH"
BASE COUNT          124 a           64 c           57 g          105 t
ORIGIN      
        1 gcttaagttc aaacttagct tacactatca agattattcg atactttttc atcaagaagc
       61 tcaccaaagg tgaactagtc ggaattatag atagttttcc tagtccatta tgtaaaccag
      121 ttagaaatag acaagcaaaa ctattgaatc aaatagccaa agttggctat aactcaacaa
      181 aaaaatctta tttttatggt cttaaaattc atatgatcgt tactaagact ggctttccga
      241 ttacttactc aatcacgaat ccaggtgtcc atgacgtgaa agtgttagag actttatcgg
      301 aagaagcgaa tcttcccaat atcctagggg acaaaggata tattagccat
//
LOCUS       DAFFFQ010000259          337 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000259
VERSION     DAFFFQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.270"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            122..328
                     /locus_tag="LQB69_003102"
     CDS             122..328
                     /locus_tag="LQB69_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL3527173.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          120 a           43 c           82 g           92 t
ORIGIN      
        1 gataaaattg aacaactgga cgggcaaatt gatgccttgc tttcagaaag agaaatattg
       61 atagaagaaa acaagcgatt gaaaaagaaa gtagcccaac caatgaaggg aggtggatag
      121 tatgaacaat agaacctttg atattttgaa atggatcgct atagtagtta tccccgcttt
      181 agcgactttt gtcggtcttg tcggaaaagg tgttaactgg cggtacacag atataacggt
      241 gatgattatt acaggttttg gagcatttct agggagtatt ttaggtgtat caaatcgaac
      301 gtataagaaa atttcatcag ataaatagga ggataga
//
LOCUS       DAFFFQ010000260          321 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000260
VERSION     DAFFFQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.271"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(69..321)
                     /locus_tag="LQB69_003103"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="LQB69_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAFFFQ010000261          316 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.272, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000261
VERSION     DAFFFQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.272"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>316
                     /locus_tag="LQB69_003104"
     CDS             <1..>316
                     /locus_tag="LQB69_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3527174.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSH"
BASE COUNT           80 a           66 c           83 g           87 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatc
//
LOCUS       DAFFFQ010000262          312 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000262
VERSION     DAFFFQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.273"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            22..>312
                     /locus_tag="LQB69_003105"
     CDS             22..>312
                     /locus_tag="LQB69_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3527175.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRA"
BASE COUNT          114 a           59 c           62 g           77 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg ca
//
LOCUS       DAFFFQ010000263          308 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000263
VERSION     DAFFFQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.274"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            87..275
                     /locus_tag="LQB69_003106"
     CDS             87..275
                     /locus_tag="LQB69_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527176.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          114 a           54 c           48 g           92 t
ORIGIN      
        1 ttacaattta aacatttgac aaactataga acagcactta aaatgtgtat acaagtgaca
       61 gcgctttaaa aaaagaggtg cagcctatgt taaaaaaaac tatgttggta ctaggttcta
      121 tttctctagg agtattaggt tttagcccca tgctattatc cgtaattgga aaaaataatt
      181 ccaaagggga acttaaatta aatccaaatt actacaatag tcctaatgaa cctatgttta
      241 tttcagaaaa acaaaacgac ggtcaggaca aataatcctg ccgtcgtttt gctctaactt
      301 ctaccagt
//
LOCUS       DAFFFQ010000264          296 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000264
VERSION     DAFFFQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.275"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>296)
                     /locus_tag="LQB69_003107"
     CDS             complement(<1..>296)
                     /locus_tag="LQB69_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL3527177.1"
                     /translation="DWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSK
                     SAMYIADWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESG"
BASE COUNT           99 a           57 c           46 g           94 t
ORIGIN      
        1 tcaccgcttt ccaacatgaa aggaataaca ccagcaacat ataaatttgt atcaaattct
       61 ttcacaaaaa agtccatata ctcaagatat tgcatcgcat ttctcatcga ttttacttgg
      121 gtctgtaaga cgacgattgc ccagtccgca atgtacatcg cggatttact atatacagaa
      181 atagaaggtg ggacatctaa atagatgacg tcaaactcat caaataatgg ttctagcttt
      241 tctttcatat atttgaattg ggataatgaa tcattatatt ggtctttgat ccaatc
//
LOCUS       DAFFFQ010000265          266 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000265
VERSION     DAFFFQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.276"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            85..>266
                     /locus_tag="LQB69_003108"
     CDS             85..>266
                     /locus_tag="LQB69_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL3527178.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           84 t
ORIGIN      
        1 tacctatgaa atattttcac gtgttaaagt attcctcgtt gaacacaaca aataaaactt
       61 tttaacgaat aaaggagatt tttcatgaca tcacttaaaa ctttactttt tggaacaact
      121 ttggctgccg gcgctgcatt cttcatggga acgactgctc atgcggacga agcttatacc
      181 gttcaatcag gtgatactct atcaacgatt tctcaaaaat acgttggtga taactccttg
      241 atcaatgcca ttgcagaatc aaattc
//
LOCUS       DAFFFQ010000266          265 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000266
VERSION     DAFFFQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.277"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>265
                     /locus_tag="LQB69_003109"
     CDS             <1..>265
                     /locus_tag="LQB69_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HBL3527179.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAFFFQ010000267          248 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000267
VERSION     DAFFFQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.278"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT           76 a           46 c           51 g           75 t
ORIGIN      
        1 tatgcttgtg atgaattgat tttcgttctg cacttttgga cgataaattt ggacaattga
       61 aaatacaaaa aaaagaaagg cccctgtaag actcgggaga ctggtaatct ccgtcagtgc
      121 cctacaggaa agcattaagc tcactcctta atgcgtgccc ttcagtgtta cggtattacc
      181 cgcaacggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
      241 ggttaaaa
//
LOCUS       DAFFFQ010000268          243 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000268
VERSION     DAFFFQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.279"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            128..>243
                     /locus_tag="LQB69_003110"
     CDS             128..>243
                     /locus_tag="LQB69_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL3527180.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT           99 a           29 c           47 g           68 t
ORIGIN      
        1 gataaaaatt caaaaagaaa aaatagtagt tatttacttg tcatgttact aaataaaaaa
       61 ataatttaaa tatgatatat agtcatttat ttagtgattt cattctaaaa taaagggaga
      121 aatgtctatg ctatacggtg caattgaagc aggcggcaca aaatttgtgt gtgccattgg
      181 cgatgaagaa atgacgatca aggaaagagt gagctttccg acaacgacac ctgaggaaac
      241 aat
//
LOCUS       DAFFFQ010000269          239 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000269
VERSION     DAFFFQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.280"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>239)
                     /locus_tag="LQB69_003111"
     CDS             complement(<1..>239)
                     /locus_tag="LQB69_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527181.1"
                     /translation="WNELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCC
                     VHVSRNIAHKVRVKDRKEICDDFKAVYQASSKEEA"
BASE COUNT           71 a           50 c           40 g           78 t
ORIGIN      
        1 tttgcctctt cctttgagct agcttgataa acagccttaa aatcatcaca gatttctttt
       61 cgatctttga cacgtacttt atgagcaata ttacgggaaa cgtgaacaca gcaatgttgg
      121 tatttagctt taggataaat ctggtgaatc gtatctttca tgccttttaa gccatcggta
      181 ataaacagta agacatcatg aacccctctg gagtaaatgt cctgtaacaa ctcattcca
//
LOCUS       DAFFFQ010000270          235 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000270
VERSION     DAFFFQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.281"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            41..>235
                     /locus_tag="LQB69_003112"
     CDS             41..>235
                     /locus_tag="LQB69_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527182.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          103 a           37 c           36 g           59 t
ORIGIN      
        1 atgtttttaa cgacaaaaac atacccagga ggacttttac atgacccaag tacattttac
       61 actgaaaagc gaagagattc aaagcattat tgaatattct gtaaaggatg acgtttctaa
      121 aaatatttta acaacggtat ttaatcaact aatggaaaat caacgaacag aatatattca
      181 agcaaaagaa tatgaacgaa cagaaaaccg acaaagtcaa cgaaatggct attat
//
LOCUS       DAFFFQ010000271          234 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000271
VERSION     DAFFFQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.282"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
BASE COUNT           91 a           41 c           32 g           70 t
ORIGIN      
        1 gttatcaggt tcctagcctt tatttaagta ttaccccaca gacccttcca tatagatttt
       61 taaattacac actagtttaa actaaaacaa acaaggaaac acttaccatt taatcataaa
      121 attagctcat atgaatatat tagaatgtaa aatagactgg tcaaaatctg gtcaaaaaag
      181 aggactaata caggtagtcc tcagactgta gacaaagtcc attttatgga tgat
//
LOCUS       DAFFFQ010000272          224 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000272
VERSION     DAFFFQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.283"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>224
                     /locus_tag="LQB69_003113"
     CDS             <1..>224
                     /locus_tag="LQB69_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL3527183.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAFFFQ010000273          221 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000273
VERSION     DAFFFQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.284"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>221
                     /locus_tag="LQB69_003114"
     CDS             <1..>221
                     /locus_tag="LQB69_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL3527184.1"
                     /translation="DGLTLDIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLG
                     SAFRKLQSVGLYTKTEHRTVKYLNNLIEQ"
BASE COUNT           74 a           44 c           47 g           56 t
ORIGIN      
        1 gacggcttaa ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct
       61 ttcttaaaac gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca
      121 ccttctcttg gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag
      181 caccgaactg tgaagtatct taacaattta atagaacaag a
//
LOCUS       DAFFFQ010000274          216 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000274
VERSION     DAFFFQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.285"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>216)
                     /locus_tag="LQB69_003115"
     CDS             complement(<1..>216)
                     /locus_tag="LQB69_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL3527185.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT           64 a           55 c           36 g           61 t
ORIGIN      
        1 tgtttcctcc ttcttcgctt tcagccgaga acattttgta ggttcgattt gatacaccca
       61 acacactccc taaaaacgcg ccaaaaccag taatgatgac aacacagata tctgtgtact
      121 gccaattgag cgctttacca actaacccca cgaaagtagc tagtgcggga ataattacca
      181 gtgcgaacca ttttagtact tcgaacgttt tattat
//
LOCUS       DAFFFQ010000275          213 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000275
VERSION     DAFFFQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.286"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..>213)
                     /locus_tag="LQB69_003116"
     CDS             complement(<1..>213)
                     /locus_tag="LQB69_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL3527186.1"
                     /translation="SEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFE
                     SILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           66 a           41 c           32 g           74 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tga
//
LOCUS       DAFFFQ010000276          207 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000276
VERSION     DAFFFQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.287"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>207
                     /locus_tag="LQB69_003117"
     CDS             <1..>207
                     /locus_tag="LQB69_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL3527187.1"
                     /translation="TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTA
                     CNEAKTANVLDRTFTQEQPLEAIVT"
BASE COUNT           67 a           47 c           49 g           44 t
ORIGIN      
        1 acccgaaaat tgaagaagtg tttagcaaag cgtgggcttc aactcagtcg gcgccgaatc
       61 ggtcgaatca tgaaacgccg cggattgaca tctacctata cgatcgctca ttttaaaggg
      121 caacgaacag cttgtaatga agcgaaaaca gcgaatgtat tagatcggac ctttacacaa
      181 gaacagccat tggaagccat cgttacg
//
LOCUS       DAFFFQ010000277          207 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000277
VERSION     DAFFFQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.288"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>207
                     /locus_tag="LQB69_003118"
     CDS             <1..>207
                     /locus_tag="LQB69_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL3527188.1"
                     /translation="AVDIENSINEWLQSVPKNLFKSITFDCGKEFSNWKSISNTNDID
                     IYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           74 a           37 c           36 g           60 t
ORIGIN      
        1 ggcagttgat attgaaaatt cgattaatga atggcttcaa tccgtaccca aaaatctttt
       61 caaatcaatt acctttgatt gtggaaaaga attctctaat tggaaaagta ttagtaatac
      121 gaatgatatt gatatttatt tcgcagaccc aggaacgcct tcccaacggg gattaaatga
      181 acattcaaac ggactcctcc gaaaaga
//
LOCUS       DAFFFQ010000278          201 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000278
VERSION     DAFFFQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.denovo.289"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..>201
                     /locus_tag="LQB69_003119"
     CDS             <1..>201
                     /locus_tag="LQB69_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527189.1"
                     /translation="ATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALEL
                     TYAKFIETKKAKTVSSEWLDYF"
BASE COUNT           75 a           35 c           38 g           53 t
ORIGIN      
        1 tgcgacgtac tatcgaacac tggaagaact gaaaaagcaa aatgcaaaat ggtttttcca
       61 agccttacta tgcttggaag taggtgtcaa accaagcact attaaacctt ctgaatacca
      121 agctttagaa ctaacgtatg cgaaatttat cgaaacgaaa aaagccaaga ctgtttcaag
      181 tgaatggtta gattattttg a
//
LOCUS       DAFFFQ010000279        12339 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.086, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000279
VERSION     DAFFFQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.086"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            126..1628
                     /gene="eat(A)"
                     /locus_tag="LQB69_003120"
     CDS             126..1628
                     /gene="eat(A)"
                     /locus_tag="LQB69_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL3527190.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1656..2222
                     /locus_tag="LQB69_003121"
     CDS             1656..2222
                     /locus_tag="LQB69_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL3527191.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            2433..3494
                     /locus_tag="LQB69_003122"
     CDS             2433..3494
                     /locus_tag="LQB69_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL3527192.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3497..4393
                     /gene="murQ"
                     /locus_tag="LQB69_003123"
     CDS             3497..4393
                     /gene="murQ"
                     /locus_tag="LQB69_003123"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL3527193.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4407..6389
                     /locus_tag="LQB69_003124"
     CDS             4407..6389
                     /locus_tag="LQB69_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL3527194.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6454..6900)
                     /gene="nrdI"
                     /locus_tag="LQB69_003125"
     CDS             complement(6454..6900)
                     /gene="nrdI"
                     /locus_tag="LQB69_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL3527195.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            7111..8535
                     /locus_tag="LQB69_003126"
     CDS             7111..8535
                     /locus_tag="LQB69_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL3527196.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8716..9489)
                     /locus_tag="LQB69_003127"
     CDS             complement(8716..9489)
                     /locus_tag="LQB69_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL3527197.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9486..10391)
                     /locus_tag="LQB69_003128"
     CDS             complement(9486..10391)
                     /locus_tag="LQB69_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL3527198.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(10545..10865)
                     /locus_tag="LQB69_003129"
     CDS             complement(10545..10865)
                     /locus_tag="LQB69_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL3527199.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11339..12025
                     /locus_tag="LQB69_003130"
     CDS             11339..12025
                     /locus_tag="LQB69_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL3527200.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12027..>12339
                     /locus_tag="LQB69_003131"
     CDS             12027..>12339
                     /locus_tag="LQB69_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HBL3527201.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KL"
BASE COUNT         3936 a         2163 c         2653 g         3587 t
ORIGIN      
        1 gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg tgtgtatcca
       61 acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat
      121 ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat
      181 tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac
      241 gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag
      301 ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac
      361 gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag
      421 agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg
      481 ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt
      541 taatcgatga accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt
      601 tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag
      661 tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct
      721 ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat
      781 tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt
      841 cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag
      901 tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg
      961 tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata
     1021 tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag
     1081 atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc
     1141 atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc
     1201 ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca
     1261 ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga
     1321 aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag
     1381 atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg
     1441 ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg
     1501 atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac
     1561 tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga
     1621 gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg
     1681 aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag
     1741 gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga
     1801 aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc cattcccctc
     1861 ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg ccgctgaccg
     1921 ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca agcaaagatg
     1981 agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg gagtcagtct
     2041 tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac gaagaaaatg
     2101 tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat tttcatccga
     2161 tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt tcttatgagt
     2221 aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg cttagcagtg
     2281 ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag aaatgaaatt
     2341 taattatttt aataaaagta ttggaatttt gtttctttag atatatactc tagtcataga
     2401 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
     2461 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
     2521 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
     2581 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
     2641 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
     2701 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
     2761 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
     2821 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
     2881 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
     2941 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
     3001 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
     3061 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
     3121 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
     3181 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
     3241 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
     3301 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
     3361 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     3421 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     3481 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     3541 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     3601 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     3661 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     3721 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     3781 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     3841 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     3901 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     3961 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     4021 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     4081 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     4141 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     4201 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     4261 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     4321 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     4381 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     4441 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     4501 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     4561 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     4621 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     4681 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     4741 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     4801 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     4861 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     4921 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     4981 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     5041 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     5101 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     5161 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     5221 tagatatcat cgttacacca actctttcgt tgattgccat cggtctgatt gaaattttct
     5281 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     5341 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     5401 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     5461 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     5521 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     5581 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     5641 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     5701 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     5761 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     5821 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     5881 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     5941 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     6001 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     6061 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     6121 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     6181 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     6241 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     6301 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     6361 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     6421 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     6481 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     6541 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     6601 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     6661 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     6721 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     6781 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     6841 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     6901 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     6961 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     7021 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     7081 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     7141 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     7201 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     7261 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     7321 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     7381 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     7441 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     7501 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     7561 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     7621 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     7681 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     7741 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     7801 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     7861 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     7921 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     7981 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     8041 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     8101 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     8161 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     8221 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     8281 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     8341 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     8401 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     8461 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     8521 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     8581 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     8641 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     8701 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     8761 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     8821 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     8881 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     8941 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     9001 catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag tataagtgaa
     9061 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     9121 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     9181 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     9241 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     9301 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     9361 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     9421 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     9481 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     9541 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     9601 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     9661 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     9721 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     9781 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     9841 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     9901 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     9961 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
    10021 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
    10081 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
    10141 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
    10201 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
    10261 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
    10321 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
    10381 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
    10441 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
    10501 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
    10561 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
    10621 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
    10681 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
    10741 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
    10801 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
    10861 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
    10921 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
    10981 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
    11041 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
    11101 gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct tatttctata
    11161 tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat aatttttgtg
    11221 ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa acaactatat
    11281 tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag gaaaatatat
    11341 ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg catttaaatt
    11401 agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg gcggtcttgg
    11461 cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg ggatggcgat
    11521 ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga aagaatcagc
    11581 ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag atatttatgg
    11641 gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg ctggatttgt
    11701 tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt tagaagtcca
    11761 tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg tctgctggtt
    11821 ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat caacactgaa
    11881 agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc cagtggctat
    11941 cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat tagcgaagaa
    12001 atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat attttattag
    12061 gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt tacactggag
    12121 taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt ttctggacgg
    12181 tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac ggtggcttga
    12241 tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa agccggcttc
    12301 catcaaaaga atatatggaa aaaatgtcag acaaactag
//
LOCUS       DAFFFQ010000280         8780 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.099, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000280
VERSION     DAFFFQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.099"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            108..2417
                     /locus_tag="LQB69_003132"
     CDS             108..2417
                     /locus_tag="LQB69_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL3527202.1"
                     /translation="MIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKI
                     DEQTEALISMIGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLM
                     GDFVSMPMAEIERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYD
                     YHLPKRYEDKKVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELD
                     FPNSEIFYYQQQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETS
                     TNVVDALESVDELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIK
                     LVRPFGDMLGLHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNL
                     DTGRNIYLQPSLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIV
                     DPKAERGEWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFL
                     TGISSRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYD
                     FAHLLFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTF
                     ALDFIHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLL
                     DEKMKNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGR
                     VGVMQFHPIFEELLHAFDTRPPVRNEVEE"
     gene            2414..4441
                     /locus_tag="LQB69_003133"
     CDS             2414..4441
                     /locus_tag="LQB69_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL3527203.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            4438..5460
                     /locus_tag="LQB69_003134"
     CDS             4438..5460
                     /locus_tag="LQB69_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL3527204.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDGETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            5477..6406
                     /locus_tag="LQB69_003135"
     CDS             5477..6406
                     /locus_tag="LQB69_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL3527205.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            6651..6767
                     /locus_tag="LQB69_003136"
     CDS             6651..6767
                     /locus_tag="LQB69_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL3527206.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            6783..8702
                     /gene="tet(M)"
                     /locus_tag="LQB69_003137"
     CDS             6783..8702
                     /gene="tet(M)"
                     /locus_tag="LQB69_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL3527207.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT         2930 a         1493 c         1770 g         2587 t
ORIGIN      
        1 tatttaatca tgatggcgaa gtattcgctt attatgaatt aattccctat aattacagtt
       61 ttttaagttc cgatgaaaaa attcaagttc atgataattt ctgtcaattg attgcccaaa
      121 atcgtgatgg caagattcat gctctgcaac tagcaaccga atcaagtatt cgagccacac
      181 aggaacaatc gaaaaaagaa atcactggac gattaaaaga gatagcttac gaaaaaatag
      241 atgaacagac cgaagcgtta atttctatga ttggtgacaa tcaagtagac tatcgctttt
      301 ttattggttt taaactctac ctcaatgaac aggaagtatc cgtaaaaaat gttgggaaag
      361 aaattcaaaa agcatttgcg gaattctttc atggtgtgaa tcacaaatta atgggcgatt
      421 ttgtttctat gcctatggcg gaaatcgaac gcttttctaa gatggaacag ttgttagaaa
      481 gtaaattatc cagacgcttt cagattcgta aattggagaa agatgatttt ggttatatta
      541 ttgaacatct ttacgggcaa acaggtgtcc cttttgatga ctacgactac catttaccga
      601 aacgatacga agataaaaaa gtgttggtaa agaaatacga tattctcaaa cccactcgct
      661 gtttaatcga agaaaatcaa cgctatttac ggattgaaca ggaagatacc actacttatg
      721 tggcgtattt tacaattaat agtattgttg gtgaattgga tttccctaat agtgaaattt
      781 tttattacca gcaacagcag tttactttcc ccgttgatac cagtatgaat gttgaaattg
      841 tcgccaataa aaaagcctta tctaccgttc gcaataaaaa gaaagaactg aaagatttag
      901 atagccacgc atgggaaagc gacaacgaaa ccagtactaa tgtggtggac gctttagaat
      961 ctgtcgatga attagaaagt aatttagacc aaaccaaaga atctatgtac aaactaagct
     1021 atgtggtacg ggtatcagca aagaatttgg acgagctgaa acaacgttgt gatgaagtaa
     1081 aagattttta tgatgatctg tctatcaaat tagtacgacc ttttggcgat atgctaggct
     1141 tgcataatga atttattccc tcaagcaagc gatatatcaa tgactatatt caatatgtca
     1201 cgtcggattt tttggctggt cttggtttcg gtgctacaca aatgattggt gaaaattctg
     1261 gtatttacgt cggttacaat ctcgatacag gcagaaatat ttatctccaa cctagccttg
     1321 caagtcaagg aattaaaggc tctgtaacaa atgcattagc gtccgctttt attggttcat
     1381 taggtggcgg aaaatcattc tctaacaata tgctggtcta ttattcagta ctctttggcg
     1441 gacaggcaac cattgttgac ccaaaagctg aacgtggtga atggaaagaa accttacctg
     1501 atattgccca tgaaatcaat attgttaatc tcacgagtga gaacgacaat aaagggttac
     1561 ttgaccctta tgtgatttta aaactcccga aagattcaga aagccttgcc attgatattc
     1621 tcacgttctt aacaggtatt tccagccgag atagcgagaa gtttcctgtc ctgcgtaaag
     1681 ctattcggag tgtaactcaa agcaaacaac gagggttgtt attggtgatt gatgaattgc
     1741 gaaaagaaga tacgcccatc agcaatagta ttgctgacca tatcgaaagc tttgtcgatt
     1801 atgattttgc ccatctgtta ttcagtgatg gcagtgtcac ccaatccatt agcttggaaa
     1861 aacaactcaa tattattcaa gtggcagatt tggtcttacc cgattccaat agctcttttg
     1921 aagaatacac taccatggaa ttattaagtg tggcaatgct gattgtcatt agtacctttg
     1981 ctttagactt tatccactct gaccgttcca tttttaaaat tgttgacctt gatgaggctt
     2041 ggagtttttt acaagtggca caaggaaaag ctctatctat gcgattggtt cgagcaggac
     2101 gtgccatgaa tgcaggggtc tatttcgtta ctcaaaacgc ggacgattta ctagatgaaa
     2161 agatgaagaa taatattggc ttgaaatttg cctttcgttc cactgaccta gtggaaatca
     2221 aaaagacctt agaatttttt ggtgtggaca aggaagatga aaacaatcag aaacgcttac
     2281 gtgaattaga aaatggacaa tgtttagttt cagacatcta tggtcgtgta ggggttatgc
     2341 agtttcaccc gatttttgaa gagttgcttc atgcctttga taccagaccg cctgtgcgaa
     2401 atgaggtgga agaatgacac aaaaacaaaa gaaatttctt cactatagtt ggataacgct
     2461 cctttgtgtg ggcgttattt tacttttatt aggcacgctg ggcaatgcag tacaagcgac
     2521 aggcttagtt gatgaaacca ttgatacaag caatgaatac tccaagtatg gtttgaatca
     2581 atatcagctt gattattatg tggacaatag ctggggctgg cttccatgga attggagtga
     2641 tggcatagga caatcggtga tgtatggatt gtatgccatt actaatttta tttggacaat
     2701 ttctctttat ctatcgaacg ccacaggtta tttggtgaaa gaagcctatg ccttagattt
     2761 cattagtcaa acatcccaag caattggcga aaacatgcag acgattgcag gcattacacc
     2821 gaatggcttt tccagttcag ggttttatgt cggtttcctc cttcttttta ttctcattgt
     2881 tggaatctat gttgcctata caggattggt taagcgagaa acgacgaaag tgatacgagc
     2941 tgttttaaac tttgtcgtgg tgtttattct atcggcttcc tttattgcgt atgcacccga
     3001 ttatattgcg aaaatcaatg atttttccaa agatgtcagt accgccagtc tgaatattgg
     3061 gacaaaaatt gttcttccta acacggatag tcaagggaaa gacagtgtgg acatgatacg
     3121 tgacagctta ttctctgttc aagtaaagca accatggcta ttgcttcaat atggctccac
     3181 tgatgtagaa gcgttagggg aagaacgtgt ggaaagttta ttgtctacca gtccagatac
     3241 aaacaatggt gaagatagag aaaatgtagt gattgaagaa attgaagatc atgacaatac
     3301 ctatttaacc ctaccgaaga cgatttccag attaggaaca gtcttctttt tgtttgtatt
     3361 caatattggt atctcgattt ttgttttcct tctaacagga atcatgattt tttcgcaagt
     3421 gctatttatt attttcgcta tgtttttgcc aatcagtttc ctattaagta tgttgccgag
     3481 ttttgatggc atgggcaggc atgccattac caagttattt aatgtgatta tgacaagggc
     3541 tggtattacc ttgattatta ccgttgcctt tagtatttcc actatgcttt attcgctgtc
     3601 agcaagttct ccctttttca tgattatgtt tttacaaatt gtgacctttg cagggattta
     3661 ttttaaactg ggcgatttaa tgagcctatt cactttacaa agcaacgact ctcaaaacat
     3721 gggcagacaa gtgtttcgca gacctcgtca aatgatgaat cgacaaacca gaagatttca
     3781 acgcagtatg agtaagattt ttcgtggaaa agcagggtca acgaagcaaa agacaatgac
     3841 aacaaagcag aatcaaacca ataaagctaa ccatactcgt caaaatgaac ggactacacc
     3901 aaagaaagaa acagcacttt ccaatgtgaa aaagcaacaa ggcggtatcg ggcaacgatt
     3961 aggtgaaaag acagcccatg tattggacac gaaaaatcgt ctagtcaata aaagaaagca
     4021 ggcaaaacaa cagatacagg cgacaccaac ccatgtcaaa tatgcccttc atcaaggaaa
     4081 agagaatgtc actcataatg taacagactt taaacaatct atggcagata ccaaagctca
     4141 acgcaaacaa cagcgtatga accagcagga gcaaaaacga aagaacattg ctcaaaagcg
     4201 tttggagctg gataaagcaa aagaaaaaag acgaaaagaa aacccatcat ctcctgttca
     4261 gaataaccag cagcatgttc gcaagcgtca gcaaaccgtc acgcctgttt tgtcacaaaa
     4321 cactaaacgt gaaaatcatc agcgaccatt gaaaaaagag caaaaacaaa agacaccacc
     4381 gaagcaattc aatcagagga agctaaataa aagaacggat aagcgaggac aacaaacatg
     4441 aagctaaaaa taacagtgat ttgtagtgtg gtcttctctc tcttcttttt tctcttatta
     4501 ttcctagtca ttttctttgc agatgatacg gacagtggcg aaaacaacaa ggattcttct
     4561 attccacaag gcggtgtgac cgtttcacca gaagtgttgg cacaccgtcc gttgattgaa
     4621 aaatatggta aggaatatgg gattgaagac tatgtttctt atatcttagc cattatgcaa
     4681 gtggaatcgg gcggtacagc agaagatgtt atgcaaagtt ccgaatcttt gggtttaccg
     4741 cccaacagtt tgagtaccga agagtccatc aaacaagggg ttaaatattt cagtgaatta
     4801 ttaaccagtg cagaacaaca aggagtggat atagattctg ttatccaatc ttataattat
     4861 ggcggtggct ttttaaatta tgtgaggagt catgggaaaa aatataccta tgaattagcc
     4921 gaacagtttt ctaaagagaa gtcgggtggt caaaaagcag actatcccaa tcctatagcc
     4981 atacctgtga atggtggttg gcgatataac tatggaaatc agttttatgt tcagttggtt
     5041 tcacaatacc taaccgacac ttccccaaca gaatttgatg gtgaaaccgt gcaagtcatt
     5101 atggacgaag ccttgaaata cgaagggttt ccctatgtct ttggaggggc gtcgcctacc
     5161 acctcttttg attgtagtgg cttaatacaa tgggtgtatg acaaagctgg catttctctc
     5221 cctcgtgtcg cccaagatca gtatgacgct acgcaagaaa tctctatgga agaagcccaa
     5281 gcaggcgacc ttatcttctt tcattcaacc tataatgcag gcacatatgt gactcatgtg
     5341 gcgatatatt tagagggaaa tcgcttttat catgcaggcg acccaatcgg ttatggcgac
     5401 ttaagcagtc gttattggca agaccatctg attggggcta gacgtgttat tcataactaa
     5461 gaaaggaatg aatcaaatga ttataaaaat cgagcgaaaa gaaaagaagg agaaaccaaa
     5521 gaaatctaaa aaaccgagca agcaaagaaa agttccgatg attaaagttg gcacgcataa
     5581 gaaattaacc tttgttttat gggttttatt gattggtagt gtcggatttg gaatctataa
     5641 aaattttact gccattgaca ctcatactgt ccgtgaaaca gaaatcatta aacagcagat
     5701 tgtcgatacc aaccaagtag aaagttttgt gaaatccttt gcgaaagact acttttcgtg
     5761 gcaacaatcc caagaagcca ttgataaacg aaatgaaaaa ttgacccatt atttgacgga
     5821 agaattgcag gtattaaatg aagaaatgat tcgtaaggat attcctacta gttctagtgt
     5881 gaacgatata caggtgtggc aggtttctca agtgaatgag aacacctttg aggtactgtt
     5941 ttctgttgaa caagttatta ccgaagacaa agacaaggaa actatatcgt ctagctttca
     6001 tgtggttgtc catatagatg aatcagataa tatggttatt atcaaaaatc cgacaatgag
     6061 taagaaacct caaaaatcgg actaccaacc caaacagcta gaaagtgacc atacagtaga
     6121 tacagaaacg atggacgaaa tcactagctt tttagaaacg ttcttccagc tctatcctac
     6181 cgccacagaa aaagaattga cttactatgt aagtaatcat gtgttaccta tgattaacaa
     6241 ggaatatgtt ttcgaggaat tggtgaatcc tatctttacc agaaaagata accaagtaat
     6301 cgtgaatgtc gctgtgaaat atttagatca agaaacaaag gcaacccaaa tctcgcaatt
     6361 tgagttgata ttagaaaaac aagataattg gaagattgta aaataacaaa tattggtaca
     6421 tgattacaga tactttgtaa tcatgtactc tttttgataa aaaattggag attcctttac
     6481 aaatatgctc ttacgtgcta ttatttaagt gactatttaa aaggagttaa taaatatgcg
     6541 gcaaggtatt cttaaataaa ctgtcaattt gatagcggga acaaataatt agatgtcctt
     6601 ttttaggagg gcttagtttt ttgtacccag tttaagaata cctttatcat gtgattctaa
     6661 agtatccaga gaatatctgt atgctttgta tacctatggt tatgcataaa aatcccagtg
     6721 ataaaagtat ttatcactgg gatttttatg cccttttggg tttttgaatg gaggaaaatc
     6781 acatgaaaat tattaatatt ggagttttag ctcatgttga tgcaggaaaa actaccttaa
     6841 cagaaagctt attatataac agtggagcga ttacagaatt aggaagcgtg gacaaaggta
     6901 caacgaggac ggataatacg cttttagaac gtcagagagg aattacaatt cagacaggaa
     6961 taacctcttt tcagtgggaa aatacgaagg tgaacatcat agacacgcca ggacatatgg
     7021 atttcttagc agaagtatat cgttcattat cagttttaga tggggcaatt ctactgattt
     7081 ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt tcatgcactt aggaaaatgg
     7141 ggattcccac aatctttttt atcaataaga ttgaccaaaa tggaattgat ttatcaacgg
     7201 tttatcagga tattaaagag aaactttctg ccgaaattgt aatcaaacag aaggtagaac
     7261 tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga acaatgggat acggtaatag
     7321 agggaaacga tgacctttta gagaaatata tgtccggtaa atcattagaa gcattggaac
     7381 tcgaacaaga ggaaagcata agatttcaga attgttctct gttccctctt tatcatggaa
     7441 gtgcaaaaag taatataggg attgataacc ttatagaagt tattactaat aaattttatt
     7501 catcaacaca tcgaggtccg tctgaacttt gcggaaatgt tttcaaaatt gaatatacaa
     7561 aaaaaagaca acgtcttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
     7621 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
     7681 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
     7741 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
     7801 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
     7861 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
     7921 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     7981 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     8041 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     8101 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     8161 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     8221 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     8281 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
     8341 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
     8401 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
     8461 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
     8521 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
     8581 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
     8641 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
     8701 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
     8761 aatatagatt tgaattaaag
//
LOCUS       DAFFFQ010000281         7482 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.105, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000281
VERSION     DAFFFQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.105"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            109..192
                     /locus_tag="LQB69_003138"
     CDS             109..192
                     /locus_tag="LQB69_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HBL3527208.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="LQB69_003139"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="LQB69_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL3527209.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="LQB69_003140"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="LQB69_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1647..2312
                     /locus_tag="LQB69_003141"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="LQB69_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="LQB69_003142"
     CDS             2348..2593
                     /locus_tag="LQB69_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL3527210.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="LQB69_003143"
     CDS             2696..3565
                     /locus_tag="LQB69_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBL3527211.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="LQB69_003144"
     CDS             3546..4280
                     /locus_tag="LQB69_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL3527212.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="LQB69_003145"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="LQB69_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="LQB69_003146"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="LQB69_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HBL3527213.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="LQB69_003147"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="LQB69_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL3527214.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            6927..7469
                     /locus_tag="LQB69_003148"
     CDS             6927..7469
                     /locus_tag="LQB69_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL3527215.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1568 g         2160 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa gggggttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DAFFFQ010000282         5615 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.120, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000282
VERSION     DAFFFQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.120"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            134..598
                     /locus_tag="LQB69_003149"
     CDS             134..598
                     /locus_tag="LQB69_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL3527216.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            582..1211
                     /locus_tag="LQB69_003150"
     CDS             582..1211
                     /locus_tag="LQB69_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL3527217.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1565..3043
                     /gene="msr(C)"
                     /locus_tag="LQB69_003151"
     CDS             1565..3043
                     /gene="msr(C)"
                     /locus_tag="LQB69_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL3527218.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3090..3536
                     /locus_tag="LQB69_003152"
     CDS             3090..3536
                     /locus_tag="LQB69_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL3527219.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3735..4250)
                     /locus_tag="LQB69_003153"
     CDS             complement(3735..4250)
                     /locus_tag="LQB69_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL3527220.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4367..5569
                     /locus_tag="LQB69_003154"
     CDS             4367..5569
                     /locus_tag="LQB69_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL3527221.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1742 a          939 c         1198 g         1736 t
ORIGIN      
        1 atttcttttc tctatacttt tctattgaga gcgttttttt tattacacag ctgattcttt
       61 gacttttttc tttttttatt ctattattga caagtcaatc gttgacgagt caattattag
      121 aaaggaggaa attatgtctt tagaagagat tagtcacctt ttatatcaaa tcaaattagc
      181 cgatcaaaaa atcgtccaga tctttgaaga aaatatcggt ataagtctta ctagatacga
      241 gatcatgatg agactgaaag aagaaaatcc attgctacaa agcagtttgc aagaaaagtt
      301 gcagattgat caaggagcag ttactcgtca tctgaaggta ttggaagcaa atggctatgt
      361 ttcgcgtcag agaaatcctc agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc
      421 ccagaagaaa atcgagcatt gtgaaatgga ccataaagat acaggttccg tgctatcccc
      481 ggctttcagc gatgtagaaa tgaaacaatt tctaacactt ttagataaat tgaataccca
      541 actttgcaat ataacaacag aaacgaaaga ggttaataat aatggaacaa aaaaatgatt
      601 tttcaacaat tatgactggt agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc
      661 cacacgaaga aatgctggaa atgatcaaag aagcgacgac tgcaccttct tctgtcaata
      721 tgcaaccatg gcgctttgtg gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt
      781 tgatccgttt caatacgaga caaaatgata cttcttctgc aatggtcatg atttttggcg
      841 atatgcaatg ttacgaatat ggcgaagaaa tctataacca agcttacgaa tcagggaaga
      901 tgccaaaaga agtcagagat caacaattag ctgcgatcat tccttattac aagagttttt
      961 caagagaaga aatgaacgat gtagtcaaag tcgattctag cttagcggct atgcaattca
     1021 tgttagttgc gcgtgatcat ggctatgaaa caaatccaat tggtggattc gaagcagatc
     1081 agttggcaga ggcatttggt ttggataaag accgttatgt tccagttatt attttatcaa
     1141 taggaaaagc ggtagaagaa ggttacgagt ctgttcgttt agcaccagaa aagatcacta
     1201 cgttcgaata aaaggttttc agttactatt cagtagctat tgcttttttt gaatcgtgtg
     1261 tggtaaccta ataaaaaagg aggtcggaaa aatgactgca tcgatgaaat tacgtttcga
     1321 acttttgaat aacaactaaa taaattcaaa agacatgtat aaacaacaat ttcatggatg
     1381 gagtatgtcc attttttcga acgacagtag aataggtgaa agctatgaca gatttggtga
     1441 tttagtcata gttatttcgt tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca
     1501 gatttttctg ctctttttta gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa
     1561 acatatggaa aatttagcag taaatataac aaatctacaa gtcagctttg gcaaccagct
     1621 agaactatct attgattctc ttcgtgtcta tcagcaagat cgtataggga tcataggaga
     1681 aaatggtgtc ggtaaatcga ctttgctcaa actaatagcc ggtgaacttt ttcccgatca
     1741 tgggaaaatc caaacagaga tcaccttcaa ctacctgcct caattaacct atctcgctga
     1801 agcaaaggac ctaaatttgg aattagccag tcatttccag ttaagactgg aagaaacttc
     1861 agagcggaaa tggagcggag gggaagaacg aaagatcgag ttgatacgtc ttctttcttc
     1921 ttatgaacaa gggatgcttc tagacgagcc aacaacccat ctagatagaa aaagtattga
     1981 tcgactgatt gaagagcttc gttattatta tggtacgctg gtttttgtta gtcatgatcg
     2041 ctattttcta gatgagctag catcgaaaat ctgggaagta aaagacggag aaatccgaga
     2101 gttttcgggg aattatagtg cctatctcac tcaaaaggaa ttggagaaaa agactcagct
     2161 acgagaagca gagtcgatca tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa
     2221 gaaaaaacaa gcggaaaagt tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag
     2281 accggatcgg ttgtcttcct ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa
     2341 tgcgaaaaca ttagagagaa gactccaaaa aataggagaa acaaccaagc cgcagcagat
     2401 gaaacaaatc cgttttccag taccaaaatc tcttgaactc cacagccgct atcccatcat
     2461 gggacaaaat gtccaattgg aaagaagtgg gagaacatta ctggtaaatg gtgattttca
     2521 gttttcttta ggtaaaaaaa tcgcgattgt cggtgaaaat ggttcaggta agacaacctt
     2581 attagaacat atccgcaaac aaggagaagg aatccttctc tctccgaaag taagctttca
     2641 agtatatcag caaaagggtt atcaaatgac atctgaagaa tccatcattc gttttgtcat
     2701 gagacaaaca gagttttcag aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc
     2761 tcaggaaact ttgacgaaac cgttatgtac attaagtggg ggagaagcga cccgtctgac
     2821 gattgctttg ctttttacta agccaagtaa tgtgttgctg ttagatgaac cgactaattt
     2881 tattgatatg gcaacgatcg aagctttaga gaagctaatg caaatatatc cgggaacgat
     2941 tttgtttact tctcatgatt cttactttgt cgagcgtacg gctgatgaag tttatgaaat
     3001 aaaagggcag aaaataaaaa aagtacttac gagaaatttt taaatgtgct acgatgattt
     3061 tcgtgacaaa tttaaaggag tggtacagat tgcctacaaa taaaaaagaa ggaataattt
     3121 ttacaacaat gatgtgtttt ttgatggtct tggggatgag cgcgtataat ttatggctgc
     3181 atcaagatct tcatttatca agtttgttga ttggtctagt gcctggattc gtggtggctt
     3241 tcattttgga tgtcttcatc atcggtgtgc tggcgaaaaa aattgccttt aaactaccag
     3301 taaatccaga aagcaaggtc caaatgatct taactatttc atgcttgatg gttttaggaa
     3361 tggtgacatg tatgtcttta ttcggtgtga tcatggaaca aggaatacct gatcaattat
     3421 tctctgctta tatgaatgct tggaaaatga atgtgattgc cgctttaccg ttgcagttga
     3481 tcatcgttgg cccgctttca agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga
     3541 acactgttat ttgaaaaaag gccttggcat gctttttgtc atagtctctt gaatgcagtt
     3601 ctagaaaaat agtgatccga acgataggtt ttggcctgtc gttcggatcg ctttttcttt
     3661 tccaactgta ttgctctttt attgaagata atttgctctt caatagatat ttctagaaag
     3721 gttctgttta tcccttactc aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat
     3781 tggagttcct gctgacaaag cataattctg cgctggatca caacataacg tccaaattct
     3841 tgtttcttgt ctagcgagag gtcattcaat tttttttgat ttttttgcat gattttgttc
     3901 agctgttgtt ctctagtgaa tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa
     3961 gcatagattt ttgctagaaa ttcatctttt tgcgtaggag cgggagtttc ctctttgatc
     4021 cattcctcta caagagcacg tccttcatca gtcaagtggt agatcttttt ttgagagtgc
     4081 tcgtctttgt ccaaaacggt cacataaccc tgctggtcta acttggcaag cgttgtatag
     4141 atctgactgt gatgggcttc ccaaaaaatt gtgatcagct gcttcaattc atacccagaa
     4201 cgtggtttgg ataaaagcac gcataataat atataggcta aagtattcat aaaaatccta
     4261 cctctcgtct tattttcttt attatatcac agtaaagttt ccacgtttta catgtgcata
     4321 tttacatgta aaataaatca tgtatgatgc taatagagag aaagggatgg aaatattgga
     4381 gaatcaaata gatatacaag aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct
     4441 agttgtagca gctacctgta tgatcgcttt tatgggagtg ggtctagtag acccaatctt
     4501 aaaaacgatt gctgcacagt taaacgctac gccagctgaa acgaccctgt tatttactag
     4561 ttacatgtta gtcactggtg tggtgatgct atttacaggt ttcttatcta gtcggatcgg
     4621 tttgaaaaaa acattgatga caggattgtt tatcatcgtt gtattcgctg gtcttggcgg
     4681 attatctggg aatatcggaa tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc
     4741 tttatttgtc tctactgctt tagcaacgat cgtcagtatt ttagttggta acacagaaaa
     4801 agcaatcatg atgtatgaag cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg
     4861 tggggtcttg ggcagtggtt catggcgtta tccatttttt ggggtagcat ttttgatgtt
     4921 ttgcggtttt atagccctag cggtactttt acctgaaact gagaaaccaa agagaaaagt
     4981 aggcctttgg gaaggtgtca aagcgttaag caatcataaa cttcgttcag ttggactaat
     5041 cgcattgtta tataatttcg gattctttac tttacttgcg tatgcgccat ttttattgat
     5101 tggctattct gaattagaag tcggttttgt attttttggc tggggtgtgc ttctggctat
     5161 tgcttcgatt ttcgttgcac cggctcttga gcggaagttt acgacttatc ggacaatttt
     5221 agcagcattt gtcttattca ttatttgttt gctgttatta ggagctggaa cttctatgcc
     5281 agtccttgta ccgatcagca ttgtgttagc aggatttttc caaggaatta tcaatacact
     5341 gttgacgacg attgctatgg aaataccagg tctgcaacga aacgttgctt cgtccagcta
     5401 tagttttgtc cgctttttcg gtggagcttt agcaccattt attgcaggtg tgattggtga
     5461 acatattggt gaaagctatg cgttttattt tgcaggtgcc attgttctta tcagtgtatg
     5521 ttgtatcatt tatcaccgta attattttgc tacaaatgaa gaagaataag aaatcagagg
     5581 ttgtgaaaaa gctgtcctgc atcaagtcat aagaa
//
LOCUS       DAFFFQ010000283         4995 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.126, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000283
VERSION     DAFFFQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.126"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(30..2180)
                     /locus_tag="LQB69_003155"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="LQB69_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="LQB69_003156"
     CDS             2326..2901
                     /locus_tag="LQB69_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL3527222.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="LQB69_003157"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="LQB69_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBL3527223.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="LQB69_003158"
     CDS             3788..4942
                     /locus_tag="LQB69_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HBL3527224.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DAFFFQ010000284         4096 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.146, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000284
VERSION     DAFFFQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.146"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            545..1018
                     /gene="dfrF"
                     /locus_tag="LQB69_003159"
     CDS             545..1018
                     /gene="dfrF"
                     /locus_tag="LQB69_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL3527225.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1145..1801
                     /locus_tag="LQB69_003160"
     CDS             1145..1801
                     /locus_tag="LQB69_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL3527226.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1983..2513
                     /locus_tag="LQB69_003161"
     CDS             1983..2513
                     /locus_tag="LQB69_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL3527227.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            3059..3301
                     /locus_tag="LQB69_003162"
     CDS             3059..3301
                     /locus_tag="LQB69_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL3527228.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            3298..3768
                     /locus_tag="LQB69_003163"
     CDS             3298..3768
                     /locus_tag="LQB69_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL3527229.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            3896..>4096
                     /locus_tag="LQB69_003164"
     CDS             3896..>4096
                     /locus_tag="LQB69_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3527230.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLF"
BASE COUNT         1482 a          534 c          834 g         1246 t
ORIGIN      
        1 tatcccacat tcgcgacctc aggcttcagc ttagtatttt tcttgttatt aggcggtttg
       61 ctatggttta tccctgtggc attgtgcgca gcagagatgg caacggtgaa aggatgggaa
      121 aaaggtgttt cccaaacaaa aagatattaa gaaacagccc atgtttacct gcttgcaaac
      181 aaaagaatat tgctactaga ctaagagata ctacataagt gtgttggtat ctcttttttg
      241 ttgccgtgaa acagcagact atttcatttc tgcttgatta ccttaaaact tattagtcta
      301 tggtttgtca agggcttgtt gcgtgagaaa tgctgaatat caccctttac aaaacatagg
      361 ctgataagta acttttcaaa agcagaaaag aaatggcaat gcttataaca atatgatata
      421 atgcagatga agacaaaaaa ggaatttgtc gagctggtta gttcgagata attttgagtt
      481 tgttaagatg tggtgcactt gatggaatca caggtacgac atcatattat ggaggatgaa
      541 atagatgata ggtttgattg ttgcgaggtc aaagaataat gttataggca agaatggtaa
      601 tataccatgg aaaataaagg gagaacaaaa gcaatttaga gagttaacaa cgggtaatgt
      661 ggttattatg gggcgaaagt cttatgaaga aatcggtcat ccgttgccta atagaatgaa
      721 tattgttgtt tccaccacaa cagagtatca aggagataat ttagtttcag ttaaatcatt
      781 agaagatgca ttattattgg ctaaaggacg agatgtatac atatctggtg gatatggact
      841 atttaaggaa gctttgcaaa tagtagataa aatgtatatc acagaagtag atttaaatat
      901 tgaagatgga gatacattct ttccagaatt tgatatcaat gattttgaag ttttgatagg
      961 ggaaacactt ggtgaggaag tgaaatatac gagaacattt tatgtaagga aaaaatgaat
     1021 tgagtaaatt ttggatttag ggtgttttca taaatatatt gctttttatg catatataaa
     1081 ttgttgtaag acaaattcca gtttgtatgg ggcgataaaa tcagaatttg agagaggatt
     1141 gaatatgaat aaggaatggt ctgaacttaa taaaacaatg caggcacaaa taaaaaagaa
     1201 agatacttat aagaggggta ttgatacttt acttacttta cgaagtcagt taatacaaac
     1261 cttagtatct ttcaaagaag aattatgcag agaagatttt aactcaatcc ccttcataaa
     1321 cgctgatggt tatcatagta agacgattgc ttattctatt tggcatattt tccgcattga
     1381 agatatagtt gtgcatacag tgataaatga agatgaacaa gtattttttg caggcaatta
     1441 tcaagagcgt atcaattcac ctatcattac aacaggaaat gaactcatga aacagcagat
     1501 tgcagacttt tcaaaacagc ttaatctgga agaattatat ttatatattt tcgaagtatg
     1561 ggaaagcact gagaaaatgc ttgaacggtt atcttatgat gaattgaaaa gaaaaatacc
     1621 gaaagaaaga aaaggatact tagaatcgtt gaatgtagta aacgacaatg aaaaagcaat
     1681 ttggctgatt gattattggt gtaataaaga tatttgtgga ctaattcaaa tgccgttttc
     1741 aagacattgg attatgcaca cagaagcctg tttgcgtata aagaacaaga tacattcata
     1801 ggcaaaaatt tcagtttgta gaactaagga aattggaatt taaggaggaa taaagtatgg
     1861 ttagtgattt tataagagca gcatttccat ggattttaat gggtttattt gtagcaatca
     1921 gttgttccct tatgagtaaa aaagagaaat aaattcctgt ttgtatttat aaaagagaaa
     1981 aaatgagaaa agtagtttta tttattgcca tgagccttga cggatatatt gcggacggta
     2041 atggtggagt agcttggcta aatggtcatg gaaatgacaa tgaaaacatc gacacttata
     2101 cagaatttac caaagatata gatactgtct taatggggtg gaatacttct catcaggttg
     2161 ttactgaact ttctccgcaa gaatgggtat ataataaatt tacaacttat gtattaacac
     2221 ataaagagtg caattcttcc caacaaattc attttacaag tgaaaatcct gttttattat
     2281 tggaacggct aaaacaagaa gcaggaaagg atatttggat ttgtggcgga gcaaatcttg
     2341 ttcagcagtt ggtaagcaaa aatatgattg ataaatatta catttctgtt attcctactc
     2401 tgttaggaaa tggggttcgt cttttgggca acatagacag ggaaataaag ttaaggcttt
     2461 gtaaaacaca gacttataac ggaataaccg atttgattta tatgcgtaga taactagaga
     2521 gaaacagaaa agctgttaaa ccgacggagc gatccgacca cgttggcggg aaaatcctta
     2581 agatttttcc gccatgcttg caagggggcg gttcgcttcg gcggttcggc gagcttgcga
     2641 gccgttcaaa gcccccgtta tctaacaagg ggggcacaaa aaacaagaaa cactatacac
     2701 taacaagcgg aaaacgctgt atttataagg caaaacccca tttttacagt agtgatataa
     2761 attgtaccta tatcactatg aaaattgaat agtaaatatg agaaagacag ttgatttcaa
     2821 aaaataagaa attgattgtc gttttctttt gcagaaattt aagtgcaggc aattatggta
     2881 gttgtcgtaa ttgctgtcag ctcagtgttt atcactgatg gtgtgaaaga tttacggttg
     2941 gagatgttca ataagattat tggtgcttaa ttatttttat tgtagtgctg atatgagaat
     3001 gttataattg gggtataaaa catacaaata aatttgctga aaaatgaggt atcaaaatat
     3061 ggaaaatgaa ttactggaaa acttaggaag acttcacaca actgaacttg gcgttattag
     3121 aattaaaaag aacttatctt tgaatgtaga aaatgtaatt gaatggtgca aagaaaaaat
     3181 tagcctttct aatgcggaaa tcacaagaaa aggaaaaaac tggtatataa cgattgataa
     3241 ttgtattata acggtaaatg cgtacagtta taccgttatc actgctcaca aggtaaaatg
     3301 ataaattttt ctataaagga attaaacgca tttgcagtta ttgggcagga agtagaactg
     3361 actaattatc aaaaaaagaa tattcagatt agtacacagt tttggagaaa gtttaacagt
     3421 agtttgaaaa aagcgtatct ttcacaatca ggaaattggg taaaatatgc ttttatggaa
     3481 agaagaaatg gaaaacttta ttatttctgt tctattcccc aaagaaccat taccccagag
     3541 ggctttctgt ataaagaaat accgtcttat aaatatttgg ttgtggagca tattggtgct
     3601 atggataaaa tatatgaaac ttatggcaag atttatgaag aaattattcc tagtacatcg
     3661 tatattccta taaaagatat tatcctacac tttgaaaaat acgattatcg ttttcattgg
     3721 aatagtgata attcagttat tgaaatttgg atacctattc aagattagaa gcaattttat
     3781 atccatacac acaaagcagt tagtcttagg attgactgct ttttctatac agatttttaa
     3841 tgacaggcaa ttatcaatta tggtagttgc cttttttgat tggaggaatt gaagaatgaa
     3901 tgataaaaac tttattgaag aactaagaca aaagcgtgag gaatacggag taacacaaac
     3961 aaggcttgct gttgcctgtg gtatcagccg tgaatattac aaccgcattg aaaaaggaaa
     4021 acagccattg aatgacgaat taagagaaat catagaaaaa cagattgagc gtttcaatcc
     4081 gcaagaacca ctattc
//
LOCUS       DAFFFQ010000285         2999 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.164, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000285
VERSION     DAFFFQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.164"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            245..1213
                     /locus_tag="LQB69_003165"
     CDS             245..1213
                     /locus_tag="LQB69_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HBL3527231.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="LQB69_003166"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="LQB69_003166"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HBL3527232.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="LQB69_003167"
     CDS             2243..2851
                     /locus_tag="LQB69_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HBL3527233.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          809 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAFFFQ010000286         2907 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.168, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000286
VERSION     DAFFFQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.168"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            363..1274
                     /locus_tag="LQB69_003168"
     CDS             363..1274
                     /locus_tag="LQB69_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HBL3527234.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1427..1912
                     /gene="vanZ"
                     /locus_tag="LQB69_003169"
     CDS             1427..1912
                     /gene="vanZ"
                     /locus_tag="LQB69_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HBL3527235.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2199..2528)
                     /locus_tag="LQB69_003170"
     CDS             complement(2199..2528)
                     /locus_tag="LQB69_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL3527236.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2833..>2907
                     /locus_tag="LQB69_003171"
     CDS             2833..>2907
                     /locus_tag="LQB69_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL3527237.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNG"
BASE COUNT         1015 a          433 c          522 g          937 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccattg aagatgtaag gcagagcgat
       61 attgcggtca ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat
      121 agaggggtgg tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag
      181 catgggtatc acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg
      241 aaataattga ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt
      301 taaggggaag attctagaaa tatttcatac ttccaactat atagttaagg aggagactga
      361 aaatgaagaa gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact
      421 acgttaatga agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc
      481 ccaaagaaca tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag
      541 aagaacaggt ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag
      601 aaagtgtgaa gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg
      661 ggttgcttga tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg
      721 tcaatgatgc tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact
      781 ttgatgagca aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt
      841 atagtgagca taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac
      901 gagcccctga aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt
      961 atccagagga caaaacagag ttaacaggaa ttcaatatga accatggcat attcgctatg
     1021 ttggtttacc acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg
     1081 attacctaaa agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct
     1141 tttattatcc tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga
     1201 tatcaggaaa caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta
     1261 attcaaggag gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta
     1321 ctagcacttt tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg
     1381 ctatactaat gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt
     1441 atctagagga ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt
     1501 acaatacaat attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt
     1561 tactgctact gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg
     1621 cttgcttttg aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact
     1681 ggttttaagt cttacttttg aaataattca atttatcttc gctattggag cgacagacat
     1741 aacagatgta attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag
     1801 caataagcat atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt
     1861 gctcgtatta ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta
     1921 aatcaagcaa tctgatcttt catacacata aagatattga atgaattgga ttagatggaa
     1981 aacgggatgt ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt
     2041 aaaaagctta cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag
     2101 gaattggtct ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc
     2161 cccaaactaa gatttcatag atagattttc tgataacttt aaaaaacacg aatagcggaa
     2221 aggatgtctt cattcgctaa agagtaataa actacttttc catcttgacg tgattttgcc
     2281 attccatgtt tttttaaaaa acgtaaatgg tgtgacgttg tagcgacact agcagtaagt
     2341 atttctgcta aatcacagac gcacatttcc ttataggttt ctaaagcata gaaaatttta
     2401 attctagaaa aatcgctgaa acatttccct aacacaagta agtttgaaaa atctttttga
     2461 tccaatttat tttttattcc atttactttt tctttatgta cgacagtcac ttggcaaact
     2521 tttttcattc atctcacctc acattcaaac gattatttga atgaagtata cctatttgat
     2581 cactcttttg tcaatctacc ttaattccaa tacactatta taagcaattt catgtattga
     2641 agtaaactta cttgcaagtg cgtttttgat tccaagttaa aaacatatta ttattctaca
     2701 taatttccta atcaaataaa actcctttga aaattgttct aaaggagttt tattattttg
     2761 tatttctact aaaaatagcg tacaatgtaa ttactaagta attacgaaat gtaaaataat
     2821 ggaggaaata atatgattac aaaaacaaga aaacaaggaa attctattat gttaacggtc
     2881 cctaaagaat tcgatgtgcc caatggt
//
LOCUS       DAFFFQ010000287         2015 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.183, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000287
VERSION     DAFFFQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.183"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..746)
                     /locus_tag="LQB69_003172"
     CDS             complement(<1..746)
                     /locus_tag="LQB69_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3527238.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNS"
     gene            1299..1847
                     /gene="aac(6')"
                     /locus_tag="LQB69_003173"
     CDS             1299..1847
                     /gene="aac(6')"
                     /locus_tag="LQB69_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL3527239.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          656 a          351 c          398 g          610 t
ORIGIN      
        1 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
       61 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
      121 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
      181 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
      241 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
      301 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
      361 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
      421 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
      481 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
      541 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
      601 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
      661 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
      721 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
      781 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
      841 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
      901 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
      961 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     1021 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     1081 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     1141 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     1201 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     1261 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     1321 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     1381 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     1441 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     1501 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     1561 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     1621 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     1681 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     1741 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     1801 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     1861 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     1921 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     1981 tgtgaaaaag tcgcttagtt tcgagtagta agaaa
//
LOCUS       DAFFFQ010000288         2015 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.184, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000288
VERSION     DAFFFQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.184"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            complement(<1..746)
                     /locus_tag="LQB69_003174"
     CDS             complement(<1..746)
                     /locus_tag="LQB69_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL3527240.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNS"
     gene            1299..1847
                     /gene="aac(6')"
                     /locus_tag="LQB69_003175"
     CDS             1299..1847
                     /gene="aac(6')"
                     /locus_tag="LQB69_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL3527241.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEVYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          655 a          351 c          398 g          611 t
ORIGIN      
        1 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
       61 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
      121 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
      181 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
      241 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
      301 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
      361 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
      421 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
      481 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
      541 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
      601 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
      661 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
      721 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
      781 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
      841 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
      901 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt
      961 tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta
     1021 tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc
     1081 tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct
     1141 attttgaata aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa
     1201 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
     1261 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
     1321 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
     1381 tatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
     1441 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
     1501 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
     1561 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
     1621 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
     1681 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
     1741 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
     1801 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
     1861 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
     1921 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
     1981 tgtgaaaaag tcgcttagtt tcgagtagta agaaa
//
LOCUS       DAFFFQ010000289         1650 bp    DNA     linear   BCT 23-NOV-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate BL00496_1
            SAMN20572407-rid13347643.guided.193, whole genome shotgun sequence.
ACCESSION   DAFFFQ010000289
VERSION     DAFFFQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN20572407
            Sequence Read Archive: SRR16866570
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 17-NOV-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001176405.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001176405.1
            Genome Coverage       :: 103x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/17/2021 12:09:48
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,177
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,042
            CDSs (with protein)               :: 3,042
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 33 of 75
            Pseudo Genes (incomplete)         :: 34 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN20572407-rid13347643.guided.193"
                     /isolate="BL00496_1"
                     /isolation_source="Blood Culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Ann Arbor, Michigan"
                     /lat_lon="42.285 N 83.73 W"
                     /collection_date="2015-03"
                     /collected_by="University of Michigan"
     gene            <1..1348
                     /locus_tag="LQB69_003176"
     CDS             <1..1348
                     /locus_tag="LQB69_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HBL3527242.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..>1650)
                     /locus_tag="LQB69_003177"
     CDS             complement(1478..>1650)
                     /locus_tag="LQB69_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL3527243.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          671 a          148 c          278 g          553 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta
//
